An enzyme that can promote the death of hydatid protocercariae in acidic environments

CN120536384BActive Publication Date: 2026-05-26AGRICHINA PHARM CO LTD +1
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Patent Information

Application Number
CN202510614573.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-14
Publication Date
2026-05-26
Estimated Expiration
2045-05-14

AI Technical Summary

Technical Problem

Existing technologies are insufficient to efficiently kill echinococcosis protocercariae in the acidic environment of a dog's stomach, making it difficult to break the transmission chain. Furthermore, traditional methods pose risks of drug resistance and environmental pollution.

Method used

A synthetic enzyme with the amino acid sequence SEQ ID NO:1 and the encoding gene SEQ ID NO:2 was developed. It was expressed in recombinant Escherichia coli and could efficiently kill protocercariae in an acidic environment. The preparation method included enzyme digestion, agarose gel electrophoresis, plasmid ligation, and expression purification.

Benefits of technology

In an acidic environment of pH 3.5, the synthesizing enzyme caused the protocercariae of echinococcosis to die by 81%, which is significantly higher than that of traditional methods. This directly disrupts the survival stage of the protocercariae in the dog's stomach, reducing the risk of drug resistance and environmental pollution.

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Abstract

This invention relates to the fields of biotechnology and parasite control technology, and proposes an enzyme that can promote the death of echinococcosis protocercariae in an acidic environment. This enzyme is a synthetic enzyme with the amino acid sequence SEQ ID NO:1. This invention achieves precise intervention in key links of echinococcosis transmission through synthetic enzyme technology, possessing high efficiency, safety, and feasibility, and has significant public health and veterinary application value.
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Citation Information

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