Anti-wrinkle and firming composition and application thereof

Abstract

The invention belongs to the field of daily chemicals, and provides an anti-wrinkle and firming composition and application, and the anti-aging and anti-inflammatory composition provided by the invention comprises tripeptide, chlamydomonas and a grape flower cell extract. According to the composition provided by the invention, the tripeptide, the chlamydomonas and the grape flower cell extract are compounded for use, all the components are mutually matched and promoted, and a synergistic effect is achieved in the aspects of promoting Collagen I up-regulation expression and inhibiting MMP1 expression, so that the composition has anti-wrinkle and firming effects. In addition, the composition provided by the invention can also significantly reduce inflammatory factors, such as IL-8 expression, and has an anti-inflammatory effect.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and in particular to an anti-aging and anti-inflammatory composition, an anti-aging and anti-inflammatory product and applications thereof. Background Art

[0002] Skin aging is a gradual physiological process, influenced by a variety of factors, including the environment, lifestyle, and disease. With technological advancements and rising living standards, demand for functional skincare products is growing, particularly for products with anti-aging properties. Skin aging can be categorized as both intrinsic and extrinsic. The latter is primarily caused by environmental factors such as reactive oxygen species, free radicals, and ultraviolet radiation. These factors can cumulatively damage the skin, leading to wrinkles, loss of elasticity, and dullness.

[0003] Therefore, developing an anti-aging composition has important practical significance and broad application prospects for meeting market demand. Summary of the Invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide an anti-wrinkle and firming composition, an anti-wrinkle and firming product and its application. The anti-wrinkle and firming composition provided in the present application has a synergistic effect, can significantly promote the up-regulation of Collagen I expression, inhibit the expression of MMP1 and IL-8, and has anti-wrinkle, firming and anti-inflammatory effects.

[0005] The present application provides an anti-wrinkle and firming composition comprising tripeptides, snow algae and grape flower cell extracts.

[0006] The anti-wrinkle and firming composition provided by the present application includes tripeptides, and the tripeptides, especially palmitoyl tripeptide-5, promote collagen production by activating cell signaling pathways, significantly reducing wrinkles, improving skin firmness and radiance, and showing potential in wound healing and tissue repair. The present application has no special restrictions on the tripeptides, and preferably a palmitoyl-modified tripeptide, such as palmitoyl tripeptide-5. Palmitoyl tripeptide-5, whose English name is Palmitoyl Tripeptide-5, has a CAS number of 623172-55-4, and its amino acid sequence is: Palm-Lysine-Valine-Lysine-OH, and its end-capping can be hydroxyl or salt, such as bistrifluoroacetate and mandelate. The present application has no special restrictions on the source of the tripeptides, such as palmitoyl tripeptide-5, and can be prepared with reference to WO2004 / 099237, or can be purchased directly from the market, such as Speicher, Jiakai, dsm-firmenich Beauty & Care, etc.

[0007] The anti-wrinkle and firming composition provided herein includes snow algae, which is rich in active ingredients such as carotenoids, antifreeze glycoproteins, and biopolymers, and has anti-aging and antioxidant effects. In some specific implementations, the snow algae includes 1 wt% to 2 wt% of Chlamydomonas extract, 80 wt% to 95 wt% of isomalt, 0.1 wt% to 1 wt% of lecithin, and 5 wt% to 15 wt% of glycogen. The source of the snow algae is not particularly limited, and commercially available sources are acceptable.

[0008] The anti-wrinkle and firming composition provided herein includes grape flower cell extract, which has a strong ability to self-renew and continuously divide, exhibiting anti-aging effects. In some specific implementations, the grape flower cell extract is obtained through induced culture of grape flower tissue and primarily comprises grape flower cells, along with a solvent such as glycerol. In some specific implementations, the grape flower cell extract comprises no less than 20 wt% grape flower cells. This application does not specifically limit the source of the grape flower cell extract; it can be purchased commercially.

[0009] The composition provided herein combines tripeptides, snow algae, and grape flower cell extracts. The components work together to enhance each other, achieving a synergistic effect in upregulating Collagen I expression and inhibiting MMP1 expression, thereby exhibiting anti-aging, anti-wrinkle, and firming effects. Furthermore, the composition provided herein can significantly reduce the expression of inflammatory factors, such as IL-8, demonstrating anti-inflammatory properties.

[0010] See also Figure 1 , Figure 1 This diagram shows part of the mechanism of skin aging. Under ultraviolet radiation, reactive oxygen species (ROS) cause upregulation of matrix metalloproteinases (MMP1) and inflammatory factors / mediators, leading to collagen degradation and inflammation, thus causing skin aging.

[0011] In the composition provided by the present application, the tripeptide can activate the cell signaling pathway, promote collagen production, and inhibit collagen degradation; the grape flower cell extract and snow algae can reduce the damage to skin health caused by free radicals and chronic inflammation, synergistically enhance the effect of the tripeptide, significantly enhance the anti-aging effect, achieve a synergistic effect, and far exceed the efficacy of a single ingredient in terms of anti-aging effect. Moreover, the composition provided by the present application can penetrate into the deep layers of the skin, promote collagen production, inhibit collagen degradation, and reduce the expression of inflammation-related genes, thereby comprehensively improving the health of the skin. This comprehensive anti-aging solution makes up for the defect of the limited effect of a single ingredient in the prior art, and provides consumers with a more comprehensive and effective skin care experience. In addition, the composition of the present application is reasonably compatible and can achieve significant anti-aging effects at lower dosages, reducing costs, improving cost-effectiveness, and has the advantages of high cost-effectiveness and wide applicability.

[0012] In some specific implementations, the mass ratio of the tripeptide, snow algae, and grape flower cell extract is 0.5-15:0.1-1:0.1-5. In some specific implementations, the mass ratio of the tripeptide, snow algae, and grape flower cell extract is 0.6-13:0.3-0.8:0.12-3. In some specific implementations, the mass ratio of the tripeptide, snow algae, and grape flower cell extract is 0.625-12.5:0.5:0.125-2.5.

[0013] The present application also provides the use of the anti-aging and anti-inflammatory composition described in the above technical solution in the preparation of anti-inflammatory and / or anti-wrinkle and firming products. The composition provided in the present application can be used for anti-aging, anti-wrinkle and firming or anti-inflammatory, can penetrate deep into the skin, and has significant effects.

[0014] This application also provides the use of the anti-wrinkle and firming composition described in the above technical solution in the preparation of a product that promotes type I collagen gene expression. Specifically, compared with a single dose of snow algae, a tripeptide, and a grape flower cell extract combination, the composition provided in this application produces a synergistic effect in promoting type I collagen gene expression, resulting in superior anti-aging and anti-wrinkle and firming effects.

[0015] This application also provides the use of the anti-wrinkle and firming composition described in the above technical solution in the preparation of a product that inhibits MMP1 gene expression. Specifically, compared with a single dose of snow algae, a combination of a tripeptide, and a grape flower cell extract, the composition provided in this application produces a synergistic effect in inhibiting MMP1 gene expression, resulting in superior anti-aging and anti-wrinkle and firming effects.

[0016] This application also provides the use of the anti-wrinkle and firming composition described in the above technical solution in the preparation of a product for inhibiting IL-8 gene expression. The composition provided in this application can significantly reduce the levels of inflammatory factors, such as the expression level of IL-8, thereby exerting an anti-inflammatory effect.

[0017] The present invention also provides an anti-wrinkle and firming product, comprising: the composition described in the above technical solution.

[0018] In some embodiments of the present invention, the above-mentioned products include but are not limited to personal care products, medicines, etc. Those skilled in the art will understand that, in addition to the composition described in the above technical solution, other excipients may also be included, such as pharmaceutical excipients or excipients for personal care products.

[0019] In some embodiments of the present invention, the personal care product includes: a basic care product and / or a makeup product.

[0020] In some embodiments of the present invention, the cosmetic products include but are not limited to:

[0021] (1) Foundation makeup:

[0022] Liquid foundation / cream: used to even out skin tone and cover blemishes;

[0023] BB Cream / CC Cream: A lightweight makeup base that combines skincare and finishing touches.

[0024] Concealer / pen: for covering local areas such as acne, dark circles, etc.

[0025] Loose powder / face powder: sets makeup and reduces facial shine.

[0026] (2) Eye makeup:

[0027] Eyeshadow: adds color and depth to the eyes;

[0028] Eyeliner pencil / liquid / gel: outline the eyeliner to make the eyes look more vivid;

[0029] Mascara: lengthens and thickens eyelashes, adds depth to eyes;

[0030] Eyebrow pencil / eyebrow powder / eyebrow gel: fill in the gaps in the eyebrows and shape the ideal eyebrows.

[0031] (3) Cheek makeup:

[0032] Blush: Add natural redness to cheeks and enhance complexion;

[0033] Contouring cake / stick: Use shading techniques to make the facial contours more three-dimensional;

[0034] (4) Lip makeup:

[0035] Lipstick / Lip Glaze / Lip Gloss: Change or emphasize the color of the lips;

[0036] Lip liner: Draw a clear lip boundary to prevent lipstick from overflowing.

[0037] (5) Multifunctional cosmetics:

[0038] Highlighter stick / liquid / powder: Brighten the high points of the face (such as the bridge of the nose and cheekbones) to create a radiant effect.

[0039] In addition, there are products designed specifically for special occasions, such as waterproof and sweat-proof eyeliner and long-lasting lipstick.

[0040] In some embodiments of the present invention, the basic care products include but are not limited to:

[0041] Facial Cleanser / Face Wash: Gently removes dirt, oil and makeup residue from the face;

[0042] Makeup remover oil / makeup remover water / makeup remover cream: specially designed to completely remove makeup, especially waterproof makeup;

[0043] Toner / Lotion: Use after cleansing to further cleanse residue on the skin surface, replenish moisture to the skin, restore the skin's pH balance, and lay a good foundation for the absorption of subsequent skin care products;

[0044] Essence: Contains high concentrations of active ingredients, providing deep nourishment and repair for specific skin problems (such as anti-aging, moisturizing, whitening, etc.);

[0045] Eye cream: specially designed for the sensitive area around the eyes, it can reduce fine lines, dark circles and tighten the skin around the eyes. The texture is usually light and easy to absorb.

[0046] Day / night lotion or cream: has the functions of protecting, repairing and nourishing, promoting cell regeneration, etc., which can provide the skin with necessary moisture and lock in the replenished moisture;

[0047] Sunscreen: used for UV protection, prevention of photoaging, etc.;

[0048] Facial masks: Provide extra nourishment and care to the skin, such as hydration, pore cleansing or brightening.

[0049] In some specific implementations, the personal care product includes 0.1wt%~10wt% of the composition described in the above technical solution, preferably includes 0.5wt%~9.5wt% of the composition described in the above technical solution, and more preferably includes 1wt%~9wt% of the composition described in the above technical solution.

[0050] The composition provided herein combines tripeptides, snow algae, and grape flower cell extracts. The components work together to enhance each other, achieving a synergistic effect in upregulating Collagen I expression and inhibiting MMP1 expression, thereby achieving anti-aging, anti-wrinkle, and firming effects. Furthermore, the composition provided herein can significantly reduce the expression of inflammatory factors, such as IL-8, demonstrating anti-inflammatory properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 This is a diagram of some of the mechanisms of skin aging;

[0052] Figure 2 This is a summary of the Collagen I gene test results;

[0053] Figure 3 This is a summary of the MMP1 gene test results;

[0054] Figure 4 Summary of IL-8 gene test results. DETAILED DESCRIPTION

[0055] The present invention provides an anti-wrinkle and firming composition, an anti-wrinkle and firming product, and its applications. Those skilled in the art can, drawing upon the disclosure herein, appropriately modify the process parameters to achieve these results. The methods and applications of the present invention have been described through preferred embodiments. It is apparent that those skilled in the art can modify, adapt, and combine the methods and applications herein to implement and apply the technology of the present invention without departing from the disclosure, spirit, and scope of the present invention.

[0056] The terms "comprising", "having" or "containing", including their grammatical synonyms, should generally be understood as open and non-restrictive, e.g., not excluding other unrecited elements or steps, unless otherwise specifically stated or understood from the context.

[0057] It should be understood that the order of steps or the order in which certain actions are performed are not important as long as the present invention remains operable. Additionally, two or more steps or actions may be performed simultaneously.

[0058] The use of any and all examples or exemplary language, such as "such as" or "including," herein is intended merely to better illustrate the invention and does not limit the scope of the invention unless otherwise claimed. No language in this specification should be construed as indicating any non-claimed element as essential to the practice of the invention.

[0059] In addition, the numerical ranges and parameters used to define the present invention are approximate values. The relevant numerical values ​​in the specific examples have been presented as accurately as possible. However, any numerical value inherently inevitably contains standard deviations due to individual testing methods. Therefore, unless otherwise expressly stated, it should be understood that all ranges, amounts, values, and percentages used in this disclosure are modified by the word "about." As used herein, "about" generally means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a particular value or range.

[0060] The present application provides an anti-wrinkle and firming composition comprising tripeptides, snow algae and grape flower cell extracts.

[0061] The composition provided herein combines tripeptides, snow algae, and grape flower cell extracts. The components work together to enhance each other, achieving a synergistic effect in upregulating Collagen I expression and inhibiting MMP1 expression, thereby exhibiting anti-aging, anti-wrinkle, and firming effects. Furthermore, the composition provided herein can significantly reduce the expression of inflammatory factors, such as IL-8, demonstrating anti-inflammatory properties.

[0062] The present application is further described below with reference to the following embodiments.

[0063] In the following examples, snow algae was purchased from Guangzhou Lingyu Trading Co., Ltd.; the tripeptide was palmitoyl tripeptide-5; and grape flower cell extract was derived from a Bordeaux blend red grape variety in France and purchased from Alfa Chemistry.

[0064] Examples 1 to 3 and Comparative Examples 1 to 4

[0065] The composition was prepared according to the formula shown in Table 1:

[0066] Table 1 Formulas provided in the examples and comparative examples of this application

[0067]

[0068] Test Example 1

[0069] The test was conducted in the cell laboratory of Shanghai Jiyan Cosmetics Technology Co., Ltd.

[0070] Test method:

[0071] 1. Cell inoculation: Select fibroblasts, resuscitate the cells, inoculate the cells into 12-well plates, and incubate them in a CO2 incubator (37°C, 5% CO2) overnight.

[0072] 2. Test Grouping: The experiment set up a blank control group (BC), a negative control group (NC), a positive control group (PC), and a sample group, with three replicate wells in each group. The grouping is shown in Table 2. In Table 2, the test sample in each group was diluted with DMEM culture medium (Gibco) to a final concentration that met the dosage requirements in Table 2.

[0073] Table 2 Test grouping

[0074]

[0075] 3. UVA stimulation: According to the test group, when the cell plating rate in the 12-well plate reaches 40%~50%, all groups except the blank control group are subjected to 14J / cm 2 Stimulation by UVA radiation.

[0076] 4. Dosing: After the induction period, administer the drugs according to the test group. Add 1 mL of culture medium to each well of the blank and negative control groups, and add 1 mL of culture medium containing the test sample at the corresponding concentration to each well of the sample group. After dosing, place the 12-well plate in a CO2 incubator (37°C, 5% CO2) and incubate for 24 hours.

[0077] 5. qPCR gene expression analysis (Collagen I): After incubation, cells were washed and total RNA was extracted from each group using the Tiangen Total RNA Extraction Kit, following the manufacturer's instructions. Reverse transcription was performed using TOYOBO's ReverTra Ace™ qPCR RTMaster Mix to synthesize cDNA. qPCR reactions were performed using SYBR™ Green Realtime PCR Master Mix on a real-time fluorescence quantitative PCR instrument using cDNA as a template. The 20 μL reaction system consisted of 10 μL SYBR™ Green Realtime PCR Master Mix, 0.8 μL upstream primer, 0.8 μL downstream primer (primer sequences are shown in Table 3), 2 μL cDNA template, and 6.4 μL dd H2O. Relative expression of related genes was calculated using the 2-ΔΔCt method, using GAPDH as the internal reference gene.

[0078] Table 3 Target gene primers

[0079]

[0080] 6. Effect Normalization: This test included a positive control (PC) and a negative control (NC). The PC group was treated with 100 ng / mL TGF-β after stimulation, while the NC group was stimulated without drug administration. The magnitude of gene upregulation in the PC group compared to the NC group was considered 1, and the efficacy of the other test groups was normalized to the E value.

[0081] The E value refers to the following formula:

[0082] .

[0083] 7. Statistical analysis of results: The comparison between groups was performed using t-test statistical analysis, and all statistical analyses were two-tailed. P < 0.05 was considered to be a significant difference. The results are shown in Table 4 and Figure 2 ,Table 4 is the summary of the Collagen I gene test results, Figure 2 Summary of Collagen I gene test results.

[0084] Table 4 Summary of Collagen I gene test results

[0085]

[0086] Collagen is an important indicator for judging skin youth. Figure 2 As shown, based on the UVA-induced senescent fibroblast model, the effects of different samples on the gene expression of type I collagen in fibroblasts were studied.

[0087] Compared to the BC group, the expression of Collagen I in the NC group was significantly downregulated, indicating that the stimulation conditions in this test were effective. Compared to the NC group, the expression of Collagen I in the PC group was significantly upregulated, indicating that the positive control in this test was effective. Compared to the NC group, both Examples 1 and 2 significantly upregulated the gene expression of type I collagen.

[0088] Use Jin's formula to verify whether there is synergy:

[0089]

[0090] Comparing Comparative Example 1 and Comparative Example 2 with Example 1, Comparative Example 1 is regarded as Agent A, Comparative Example 2 is regarded as Agent B, and Example 1 is Agent A + Agent B, q=39.8>1;

[0091] Comparing Comparative Example 1, Comparative Example 3 and Example 2, Comparative Example 1 is regarded as Agent A, Comparative Example y is regarded as Agent B, and Example 2 is Agent A + Agent B, q=2.8>1.

[0092] It can be seen that under the test conditions, the combined use of snow algae, tripeptide and grape flower cell extract produced a synergistic effect in promoting the upregulation of Collagen I expression.

[0093] Test Example 2

[0094] The test was conducted in the cell laboratory of Shanghai Jiyan Cosmetics Technology Co., Ltd.

[0095] Test method:

[0096] 1. Cell inoculation: Select fibroblasts, resuscitate the cells, inoculate the cells into 12-well plates, and incubate them in a CO2 incubator (37°C, 5% CO2) overnight.

[0097] 2. Test Grouping: The experiment set up a blank control group (BC), a negative control group (NC), a positive control group (PC), and a sample group, with three replicate wells in each group. The grouping is shown in Table 5. In Table 5, the test sample in each group was diluted with DMEM culture medium (Gibco) to a final concentration that met the dosage requirements in Table 5.

[0098] Table 5 Test grouping

[0099]

[0100] 3. UVA stimulation: According to the test group, when the cell plating rate in the 12-well plate reaches 40%~50%, all groups except the blank control group are subjected to 14J / cm 2 Stimulation by UVA radiation.

[0101] 4. Dosing: After the induction period, administer the drugs according to the test group. Add 1 mL of culture medium to each well of the blank and negative control groups, and add 1 mL of culture medium containing the test sample at the corresponding concentration to each well of the sample group. After dosing, place the 12-well plate in a CO2 incubator (37°C, 5% CO2) and incubate for 24 hours.

[0102] 5. qPCR Detection of Gene Expression (MMP1): After incubation, cells were washed and total RNA was extracted from each group using the Tiangen Total RNA Extraction Kit, following the manufacturer's instructions. Reverse transcription was performed using TOYOBO's ReverTra Ace™ qPCR RTMaster Mix to synthesize cDNA. qPCR reactions were performed using the SYBR™ Green Realtime PCR Master Mix on a real-time fluorescence quantitative PCR instrument using the cDNA as a template. The 20 μL reaction system consisted of 10 μL SYBR™ Green Realtime PCR Master Mix, 0.8 μL upstream primer, 0.8 μL downstream primer (primer sequences are shown in Table 6), 2 μL cDNA template, and 6.4 μL ddH2O. Relative expression of related genes was calculated using the 2-ΔΔCt method, using GAPDH as the internal reference gene.

[0103] Table 6 Target gene primers

[0104]

[0105] 6. Effect Normalization: This test included a positive control (PC) and a negative control (NC). The PC group was treated with 100 ng / mL TGF-β after stimulation, while the NC group was stimulated without drug administration. The magnitude of gene upregulation in the PC group compared to the NC group was considered 1, and the efficacy of the other test groups was normalized to the E value.

[0106] The E value refers to the following formula:

[0107] .

[0108] 7. Statistical analysis of results: The comparison between groups was performed using t-test statistical analysis, and all statistical analyses were two-tailed. p < 0.05 was considered to be a significant difference. The results are shown in Table 7 and Figure 3 ,Table 7 is the summary of MMP1 gene test results, Figure 3 Summary of MMP1 gene test results.

[0109] Table 7 Summary of MMP1 gene test results

[0110]

[0111] Matrix metalloproteinase MMP1 is a type of enzyme that degrades collagen and is closely related to skin photoaging. In the cosmetics field, in order to improve photoaging and protect collagen, active ingredients that can inhibit the expression of MMP1 are usually required. Table 7 and Figure 3The test results for MMP1 are shown, including the mean, standard deviation, p-value analysis, and E-value (power).

[0112] Compared to the BC group, MMP1 expression in the NC group was significantly upregulated, demonstrating the effectiveness of this stimulation. Compared to the NC group, MMP1 expression in the PC group was significantly downregulated, demonstrating the effectiveness of this positive control. Compared to the NC group, Comparative Examples 1 and 4 failed to inhibit MMP1 secretion, while Example 2 significantly reduced MMP1 gene expression. Furthermore, compared to Comparative Examples 1 and 3, Example 2 was significantly more effective than Comparative Example 3 (p=0.0056), while snow algae alone (Comparative Example 1) had no MMP1 inhibitory effect.

[0113] Use Jin's formula to verify whether there is synergy:

[0114]

[0115] Comparing Comparative Example 1 and Comparative Example 4 with Example 3, Comparative Example 1 is regarded as Agent A, Comparative Example 4 is regarded as Agent B, and Example 3 is Agent A + Agent B, q=23.2>1;

[0116] Comparing Comparative Example 1 and Comparative Example 3 with Example 2, Comparative Example 1 is regarded as Agent A, Comparative Example 3 is regarded as Agent B, and Example 2 is Agent A+Agent B, q=1.55>1.

[0117] It can be seen that under the conditions of this test, the combined use of snow algae, tripeptide and grape flower cell extract produced a synergistic effect in inhibiting the expression of MMP1.

[0118] These results indicate that the combined use of tripeptides, snow algae, and grape flower cell extracts can produce an MMP1 gene inhibitory effect. This synergistic effect is important for improving MMP1-induced collagen degradation in photoaging, enhancing the product's firming and anti-wrinkle effects.

[0119] Test Example 3

[0120] The test was conducted in the cell laboratory of Shanghai Jiyan Cosmetics Technology Co., Ltd.

[0121] Test method:

[0122] 1. Cell inoculation: Select fibroblasts, resuscitate the cells, inoculate the cells into 12-well plates, and incubate them in a CO2 incubator (37°C, 5% CO2) overnight.

[0123] 2. Test Grouping: The experiment set up a blank control group (BC), a negative control group (NC), a positive control group (PC), and a sample group, with three replicate wells in each group. The grouping is shown in Table 8. In Table 8, the test sample in each group was diluted with DMEM culture medium (Gibco) to a final concentration that met the dosage requirements in Table 8.

[0124] Table 8 Test grouping

[0125]

[0126] 3. UVA stimulation: According to the test group, when the cell plating rate in the 12-well plate reaches 40%~50%, all groups except the blank control group are subjected to 14J / cm 2 Stimulation by UVA radiation.

[0127] 4. Dosing: After the induction period, administer the drugs according to the test group. Add 1 mL of culture medium to each well of the blank and negative control groups, and add 1 mL of culture medium containing the test sample at the corresponding concentration to each well of the sample group. After dosing, place the 12-well plate in a CO2 incubator (37°C, 5% CO2) and incubate for 24 hours.

[0128] 5. qPCR Detection of Gene Expression (IL-8): After incubation, cells were washed and total RNA was extracted from each group using the Tiangen Total RNA Extraction Kit, following the manufacturer's instructions. Reverse transcription was performed using TOYOBO's ReverTra Ace™ qPCR RT MasterMix to synthesize cDNA. qPCR reactions were performed using SYBR™ Green Realtime PCR Master Mix on a real-time quantitative PCR instrument using cDNA as a template. The 20 μL reaction system consisted of 10 μL SYBR™ Green Realtime PCR Master Mix, 0.8 μL upstream primer, 0.8 μL downstream primer (primer sequences are shown in Table 9), 2 μL cDNA template, and 6.4 μL ddH2O. Relative expression of related genes was calculated using the 2-ΔΔCt method, using GAPDH as the internal reference gene.

[0129] Table 9 Target gene primers

[0130]

[0131] 6. Effect Normalization: This test included a positive control (PC) and a negative control (NC). The PC group was treated with 100 ng / mL TGF-β after stimulation, while the NC group was stimulated without drug administration. The magnitude of gene upregulation in the PC group compared to the NC group was considered 1, and the efficacy of the other test groups was normalized to the E value.

[0132] The E value refers to the following formula:

[0133] .

[0134] 7. Statistical analysis of results: The comparison between groups was performed using t-test statistical analysis, and all statistical analyses were two-tailed. p < 0.05 was considered to be a significant difference. The results are shown in Table 10 and Figure 4 ,Table 10 is a summary of the IL-8 gene test results, Figure 4 Summary of IL-8 gene test results.

[0135] Table 10 Summary of IL-8 gene test results

[0136]

[0137] Interleukin-8 (IL-8) is a key mediator associated with inflammation. Oxidative stress increases the secretion of IL-8, leading to the recruitment of inflammatory cells, making it a key parameter of local inflammation. Figure 4 The test results of the relative expression of the IL-8 gene are shown, including the mean, standard deviation, p-value analysis, and E-value (power).

[0138] Compared with the BC group, IL-8 expression in the NC group was significantly increased, indicating that this stimulation was effective. Compared with the NC group, IL-8 expression in the PC group was significantly decreased, indicating that this positive control was effective. Compared with the NC group, both Comparative Example 1 and Example 2 effectively downregulated IL-8 expression, and the effect of Example 2 was significantly better than that of Comparative Example 1 (p < 0.01). This shows that the combination of snow algae, tripeptide, and grape flower cell extract can better inhibit inflammatory gene expression and has positive significance for alleviating inflammatory aging.

[0139] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An anti-wrinkle and firming composition comprising tripeptides, snow algae and grape flower cell extracts.

2. The anti-wrinkle and firming composition according to claim 1, wherein the mass ratio of the tripeptide, snow algae and grape flower cell extract is 0.5-15:0.1-1:0.1-5.

3. The anti-wrinkle and firming composition according to claim 2, wherein the mass ratio of the tripeptide, snow algae and grape flower cell extract is 0.6-13:0.3-0.8:0.12-3.

4. The anti-wrinkle and firming composition according to claim 3, wherein the mass ratio of the tripeptide, snow algae and grape flower cell extract is 0.625-12.5:0.5:0.125-2.

5.

5. Use of the anti-wrinkle and firming composition according to any one of claims 1 to 4 in the preparation of anti-inflammatory and / or anti-wrinkle and firming products.

6. Use of the anti-wrinkle and firming composition according to any one of claims 1 to 4 in the preparation of a product for promoting type I collagen gene expression.

7. Use of the anti-wrinkle and firming composition according to any one of claims 1 to 4 in the preparation of a product for inhibiting MMP1 gene expression.

8. Use of the anti-wrinkle and firming composition according to any one of claims 1 to 4 in the preparation of a product for inhibiting IL-8 gene expression.

9. Anti-wrinkle and firming products, characterized by: include: The anti-wrinkle and firming composition according to any one of claims 1 to 4.

10. The product according to claim 9, characterized in that Including personal care products.

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