A green synthesis method of a cycloolefin polymer biodegradation promoter
A biodegradation promoter for cyclic olefin polymers was prepared by combining fermentation with *Bacillus licheniformis*, *Bacillus licheniformis*, and *Candida albicans* with a modified chitosan crosslinking reaction. This solved the problem of the difficulty in degrading cyclic olefin polymers and enabled efficient biodegradation and product application.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HUANXITING NEW MATERIALS (JIANGSU) CO LTD
- Filing Date
- 2025-06-03
- Publication Date
- 2026-07-10
AI Technical Summary
Cycloolefin polymers are difficult to degrade in the natural environment. Existing chemical and physical recycling methods are inefficient and complex, and no research on biodegradation has been reported.
A biodegradation promoter for cyclic olefin polymers was prepared by mixed fermentation of *Bacillus thuringiensis*, *Bacillus licheniformis*, and *Candida*, combined with modified chitosan and cross-linking reaction.
It promotes the efficient biodegradation of cyclic olefin polymers in the natural environment, improves degradation efficiency, and is suitable for cyclic olefin polymer biodegradable products.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of biodegradation accelerator technology, specifically relating to a green synthesis method for a cyclic olefin polymer biodegradation accelerator. Background Technology
[0002] Cyclic olefin polymers (COC / COP) are amorphous transparent polymers with cyclic structures. Based on different manufacturing processes, they are mainly divided into cyclic olefin copolymers (COC) and cyclic olefin polymers (COP). Cyclic olefin polymers not only possess high transparency, low birefringence, and low fluorescence, but also exhibit good heat resistance and strong thermal stability, thus enabling their widespread application in optics, medical fields, food packaging, and electronics.
[0003] However, cyclic olefin polymers possess highly stable chemical structures and a wide range of applications, making them difficult to degrade in the natural environment. The disposal of large quantities of waste cyclic olefin polymers has become a major challenge. Currently, common treatment methods are divided into chemical recycling and material recovery. Chemical recycling involves converting waste cyclic olefin polymers into smaller molecule compounds or monomers. This method not only reduces material performance but also requires strict degradation conditions, has low degradation efficiency, and is prone to causing ecotoxicity. Physical recycling mainly uses mechanical methods to reprocess waste cyclic olefin polymers into new products. This process is technically complex, has low efficiency, and the byproducts are difficult to recycle.
[0004] Existing research has found that cyclic olefin polymers can be decomposed by microorganisms in the natural environment, ultimately converting into carbon dioxide and water. This not only reduces long-term environmental pollution but also facilitates resource recycling. However, there are currently no studies or reports on biodegradation promoters for cyclic olefin polymers. Summary of the Invention
[0005] The purpose of this invention is to provide a green synthesis method for a biodegradation promoter of cyclic olefin polymers, which has the effect of efficiently promoting the biodegradation of cyclic olefin polymers.
[0006] This invention provides a green synthesis method for cyclic olefin polymer biodegradation promoters, comprising the following steps:
[0007] The complex original strain was subjected to aerobic fermentation and solid-liquid separation in sequence, and the liquid phase was freeze-dried to obtain the fermentation product;
[0008] Hydrogen peroxide was added dropwise to a chitosan-acetic acid solution, followed by solid-liquid separation, washing, and drying to obtain modified chitosan.
[0009] The modified chitosan, fermentation product, and acetic acid solution were mixed and ultrasonically dispersed to obtain an aqueous phase;
[0010] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion.
[0011] The water-in-oil reverse microemulsion system was crosslinked, washed, and dried to obtain a cyclic olefin polymer biodegradation promoter.
[0012] The composite protozoan strains include: *Citrus thuringiensis*, *Bacillus licheniformis*, and *Candida albicans*.
[0013] Preferably, the mass ratio of *Corydalis thuringiensis*, *Bacillus licheniformis*, and *Candida* in the compound original strain is 1-2:2.5-3.5:0.8-1.2;
[0014] The effective viable counts of *Candida albicans*, *Bacillus licheniformis*, and *Candida* were ≥1×10⁻⁶. 8 cfu / g.
[0015] Preferably, the culture medium used for the aerobic fermentation is water-based and includes the following substances at mass concentrations: peptone 1-4 g / L, yeast extract 0.3-0.5 g / L, glucose 3-5 g / L, potassium dihydrogen phosphate 0.3-1 g / L, magnesium sulfate 0.3-1 g / L, and trehalose 5-8 g / L.
[0016] The parameters for aerobic fermentation include: time of 24-36 hours and temperature of 28-32℃.
[0017] The mass-to-volume ratio of the original bacterial strain to the culture medium is 8-10 g: 50 mL.
[0018] Preferably, the chitosan-acetic acid solution is obtained by mixing chitosan and acetic acid solution at a mass-volume ratio of 1g:10-11mL;
[0019] The acetic acid solution has a mass concentration of 1%-1.5%;
[0020] The degree of deacetylation of the chitosan is 90%-95%.
[0021] Preferably, the volume ratio of the chitosan-acetic acid solution to hydrogen peroxide is 10-11:0.1-0.3;
[0022] The volume concentration of the hydrogen peroxide is 3%-5%.
[0023] Preferably, the mass-to-volume ratio of the modified chitosan, fermentation product, and acetic acid solution is 1g:0.2-0.3g:10-11mL.
[0024] Preferably, the method for preparing the oil phase includes:
[0025] It is made by mixing Tween 80 and white oil in a mass ratio of 1:1.12-1.15.
[0026] Preferably, the parameters for ultrasonic dispersion include: power of 600-700w and time of 1-1.5h.
[0027] This invention provides a biodegradation promoter obtained by the green synthesis method described in the above technical solution.
[0028] This invention provides the application of the biodegradation promoter described above in promoting the biodegradation of cyclic olefin polymers and / or in the preparation of products for the biodegradation of cyclic olefin polymers.
[0029] Beneficial effects:
[0030] This invention provides a green synthesis method for a biodegradation promoter of cyclic olefin polymers. By aerobic fermentation of a mixture of *Cladosporium chrysogenum*, *Bacillus licheniformis*, and *Candida*, the fermented product contains abundant enzymes and bioactive agents, which are beneficial for promoting the degradation of cyclic olefin polymers. Furthermore, by immobilizing the fermented product with modified chitosan, microorganisms and their metabolites can be fixed on the chitosan surface, promoting the full release of active functions. In addition, the stability of the modified chitosan structure is improved through a cross-linking reaction. Therefore, the biodegradation promoter provided by this invention can not only promote the biodegradation of cyclic olefin polymers but also be used in the preparation of biodegradable cyclic olefin polymer products. Detailed Implementation
[0031] In this invention, unless otherwise specified, the raw materials, equipment and methods used are all conventional selections.
[0032] When performing crosslinking, washing, and drying of the water-in-oil reverse microemulsion system, there are no special requirements; conventional methods in the art can be used.
[0033] There are no special requirements for the use of the biodegradation accelerator described in this invention; when the cyclic olefin polymer to be degraded is a particulate, powder or strip-shaped substance, it is preferable to mix the biodegradation accelerator with the cyclic olefin polymer before degradation; when the cyclic olefin polymer to be degraded is a film, it is preferable to place the biodegradation accelerator and the film in the same environment.
[0034] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0035] Example 1
[0036] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0037] (1) Preparation of fermentation product:
[0038] The compound original strain was obtained by mixing *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* in a mass ratio of 1:2.5:0.8; wherein the effective viable count of *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* was 1×10⁻⁶. 8 cfu / g.
[0039] The culture medium uses water as a solvent and includes the following substances at the following mass concentrations: peptone 2 g / L, yeast extract 0.3 g / L, glucose 4 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate 0.5 g / L, and trehalose 8 g / L;
[0040] The compound original strain and culture medium were inoculated at a mass-to-volume ratio of 8g:50mL, and fermented at 30℃ for 30h to obtain fermentation broth. After solid-liquid separation, the liquid was freeze-dried to obtain powder as fermentation product.
[0041] (2) Modified chitosan
[0042] Chitosan with a degree of deacetylation of 90% was mixed with acetic acid solution with a mass concentration of 1% at a mass-volume ratio of 1g:10mL to obtain a chitosan-acetic acid solution.
[0043] Hydrogen peroxide (3% by volume) was added dropwise to a chitosan-acetic acid solution at a volume ratio of 10 mL: 0.1 mL. After thorough stirring, the mixture was centrifuged. The centrifuged solid was then washed with water to remove the hydrogen peroxide. The cleaned solid was dried at 80°C to obtain modified chitosan.
[0044] (3) Preparation of biodegradation promoters
[0045] The modified chitosan, fermentation product, and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.2g:10mL and ultrasonically dispersed at 700w for 1.5h to obtain the aqueous phase.
[0046] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0047] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0048] Example 2
[0049] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0050] (1) Preparation of fermentation product:
[0051] The compound original strain was obtained by mixing *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* in a mass ratio of 2:2.5:1.2; wherein the effective viable count of *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* was 1×10⁻⁶. 8 cfu / g.
[0052] The culture medium uses water as a solvent and includes the following substances at the following mass concentrations: peptone 2 g / L, yeast extract 0.3 g / L, glucose 4 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate 0.5 g / L, and trehalose 8 g / L;
[0053] The compound original strain and culture medium were inoculated at a mass-volume ratio of 10g:50mL, and fermented at 30℃ for 30h to obtain fermentation broth. After solid-liquid separation, the liquid was freeze-dried to obtain powder as fermentation product.
[0054] (2) Modified chitosan
[0055] Chitosan with a degree of deacetylation of 90% was mixed with acetic acid solution with a mass concentration of 1% at a mass-volume ratio of 1g:10mL to obtain a chitosan-acetic acid solution.
[0056] Hydrogen peroxide (3% by volume) was added dropwise to a chitosan-acetic acid solution at a volume ratio of 10 mL: 0.1 mL. After thorough stirring, the mixture was centrifuged. The centrifuged solid was then washed with water to remove the hydrogen peroxide. The cleaned solid was dried at 80°C to obtain modified chitosan.
[0057] (3) Preparation of biodegradation promoters
[0058] The modified chitosan, fermentation product, and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.2g:10mL and ultrasonically dispersed at 700w for 1.5h to obtain the aqueous phase.
[0059] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0060] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0061] Example 3
[0062] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0063] (1) Preparation of fermentation product:
[0064] The compound original strain was obtained by mixing *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* in a mass ratio of 1:2.5:0.8; wherein the effective viable count of *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* was 1×10⁻⁶. 8 cfu / g.
[0065] The culture medium uses water as a solvent and includes the following substances at the following mass concentrations: peptone 2 g / L, yeast extract 0.3 g / L, glucose 4 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate 0.5 g / L, and trehalose 8 g / L;
[0066] The compound original strain and culture medium were inoculated at a mass-to-volume ratio of 8g:50mL, and fermented at 30℃ for 30h to obtain fermentation broth. After solid-liquid separation, the liquid was freeze-dried to obtain powder as fermentation product.
[0067] (2) Modified chitosan
[0068] Chitosan with a degree of deacetylation of 95% was mixed with an acetic acid solution with a mass concentration of 1.5% at a mass-volume ratio of 1g:10mL to obtain a chitosan-acetic acid solution.
[0069] Hydrogen peroxide (3% by volume) was added dropwise to a chitosan-acetic acid solution at a volume ratio of 10 mL: 0.2 mL. After thorough stirring, the mixture was centrifuged, and the solid material after centrifugation was washed with water to remove the hydrogen peroxide. The cleaned solid material was then dried at 80°C to obtain modified chitosan.
[0070] (3) Preparation of biodegradation promoters
[0071] The modified chitosan, fermentation product, and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.2g:10mL and ultrasonically dispersed at 700w for 1.5h to obtain the aqueous phase.
[0072] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0073] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0074] Comparative Example 1
[0075] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0076] (1) Preparation of fermentation product:
[0077] The compound original strain was obtained by mixing *C. chrysogenum*, *Bacillus subtilis*, and *C. candida* in a mass ratio of 1:2.5:0.8; wherein the effective viable count of *C. chrysogenum*, *Bacillus subtilis*, and *C. candida* was 1×10⁻⁶. 8 cfu / g.
[0078] The culture medium uses water as a solvent and includes the following substances at the following mass concentrations: peptone 2 g / L, yeast extract 0.3 g / L, glucose 4 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate 0.5 g / L, and trehalose 8 g / L;
[0079] The compound original strain and culture medium were inoculated at a mass-to-volume ratio of 8g:50mL, and fermented at 30℃ for 30h to obtain fermentation broth. After solid-liquid separation, the liquid was freeze-dried to obtain powder as fermentation product.
[0080] (2) Modified chitosan
[0081] Chitosan with a degree of deacetylation of 90% was mixed with acetic acid solution with a mass concentration of 1% at a mass-volume ratio of 1g:10mL to obtain a chitosan-acetic acid solution.
[0082] Hydrogen peroxide (3% by volume) was added dropwise to a chitosan-acetic acid solution at a volume ratio of 10 mL: 0.1 mL. After thorough stirring, the mixture was centrifuged. The centrifuged solid was then washed with water to remove the hydrogen peroxide. The cleaned solid was dried at 80°C to obtain modified chitosan.
[0083] (3) Preparation of biodegradation promoters
[0084] The modified chitosan, fermentation product, and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.2g:10mL and ultrasonically dispersed at 700w for 1.5h to obtain the aqueous phase.
[0085] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0086] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0087] Comparative Example 2
[0088] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0089] (1) Preparation of fermentation product:
[0090] The compound original strain was obtained by mixing *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* in a mass ratio of 1:2.5:0.8; wherein the effective viable count of *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* was 1×10⁻⁶. 8 cfu / g.
[0091] The culture medium uses water as a solvent and includes the following substances at the following mass concentrations: peptone 2 g / L, yeast extract 0.3 g / L, glucose 4 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate 0.5 g / L, and trehalose 8 g / L;
[0092] The compound original strain and culture medium were inoculated at a mass-to-volume ratio of 8g:50mL, and fermented at 30℃ for 30h to obtain fermentation broth. After solid-liquid separation, the liquid was freeze-dried to obtain powder as fermentation product.
[0093] (2) Chitosan solution
[0094] Chitosan with a degree of deacetylation of 90% was mixed with acetic acid solution with a mass concentration of 1% at a mass-volume ratio of 1g:10mL to obtain a chitosan solution.
[0095] (3) Preparation of biodegradation promoters
[0096] Chitosan solution, fermentation product and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.2g:10mL and ultrasonically dispersed at 700w for 1.5h to obtain aqueous phase;
[0097] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0098] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0099] Comparative Example 3
[0100] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0101] (1) Preparation of fermentation product:
[0102] The compound original strain was obtained by mixing *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* in a mass ratio of 1:2.5:0.8; wherein the effective viable count of *C. chrysogenum*, *Bacillus licheniformis*, and *C. candida* was 1×10⁻⁶. 8 cfu / g.
[0103] The culture medium uses water as a solvent and includes the following substances at the following mass concentrations: peptone 2 g / L, yeast extract 0.3 g / L, glucose 4 g / L, potassium dihydrogen phosphate 0.5 g / L, magnesium sulfate 0.5 g / L, and trehalose 8 g / L;
[0104] The compound original strain and culture medium were inoculated at a mass-to-volume ratio of 8g:50mL, and fermented at 30℃ for 30h to obtain fermentation broth. After solid-liquid separation, the liquid was freeze-dried to obtain powder as fermentation product.
[0105] (2) Modified chitosan
[0106] Chitosan with a degree of deacetylation of 90% was mixed with acetic acid solution with a mass concentration of 1% at a mass-volume ratio of 1g:10mL to obtain a chitosan-acetic acid solution.
[0107] Hydrogen peroxide (3% by volume) was added dropwise to a chitosan-acetic acid solution at a volume ratio of 10 mL: 0.1 mL. After thorough stirring, the mixture was centrifuged. The centrifuged solid was then washed with water to remove the hydrogen peroxide. The cleaned solid was dried at 80°C to obtain modified chitosan.
[0108] (3) Preparation of biodegradation promoters
[0109] The modified chitosan, fermentation product, and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.6g:10mL to obtain the aqueous phase.
[0110] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0111] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0112] Comparative Example 4
[0113] A green synthesis method for a biodegradation promoter, comprising the following steps:
[0114] (1) Bacterial solution:
[0115] The effective viable bacteria count was 1×10⁻⁶. 8 A compound bacterial solution was prepared by mixing CFU / mL of *C. chrysogenum*, *Bacillus licheniformis*, and *Candida albicans* in a volume ratio of 1:2.5:0.8.
[0116] (2) Modified chitosan
[0117] Chitosan with a degree of deacetylation of 90% was mixed with acetic acid solution with a mass concentration of 1% at a mass-volume ratio of 1g:10mL to obtain a chitosan-acetic acid solution.
[0118] Hydrogen peroxide (3% by volume) was added dropwise to a chitosan-acetic acid solution at a volume ratio of 10 mL: 0.1 mL. After thorough stirring, the mixture was centrifuged. The centrifuged solid was then washed with water to remove the hydrogen peroxide. The cleaned solid was dried at 80°C to obtain modified chitosan.
[0119] (3) Preparation of biodegradation promoters
[0120] Modified chitosan, composite bacterial solution and acetic acid solution were mixed evenly at a mass-volume ratio of 1g:0.2mL:10mL and ultrasonically dispersed at 700w for 1.5h to obtain the aqueous phase;
[0121] Tween 80 and white oil were mixed evenly at a mass ratio of 1:1.12 to obtain the oil phase;
[0122] The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion; a crosslinking agent (glutaraldehyde) is added for crosslinking, followed by washing and drying to obtain a biodegradation promoter.
[0123] Test Example 1
[0124] COP films (25 μm thick) were purchased online using standard procedures. The biodegradation accelerators prepared in Examples 1-3 and Comparative Examples 1-4 were used as the test subjects. The biodegradation accelerators were weighed in a 1:5 mass ratio with the COP films. COP films without biodegradation accelerators were used as blank controls (the initial mass of the COP films in each treatment was 5 g). In the same soil environment, 10 cm deep pits were set up. The biodegradation accelerators were evenly sprinkled at the bottom of the pits, and then the COP films were placed in the pits. Soil was then used to stabilize the COP films. The morphology of the COP films in each treatment was recorded on days 40 and 60 of the experiment, and the results are shown in Table 1. The mass loss due to degradation of the COP films in each treatment was recorded on days 40, 60, and 80 of the experiment, and the results are shown in Table 2.
[0125] Table 1. Morphology of COP films under different treatments
[0126]
[0127]
[0128] As can be seen from Table 1, when the COP film was biodegraded using the methods in Examples 1-3, the biodegradation promoter accelerated the biodegradation of the COP film because it promoted the activity of existing microbial communities in the soil environment. In contrast, the comparative example changed the method of preparing the biodegradation promoter, which also promoted the degradation of the COP film, but the degree of degradation of the COP film was small within the same time, which was not conducive to subsequent rapid degradation.
[0129] Table 2. Mass loss rate of COP film degradation under different treatments.
[0130] deal with 40 days 60 days 80 days Example 1 47％ 55％ 71％ Example 2 45％ 56％ 69％ Example 3 47％ 56％ 70％ Comparative Example 1 30％ 42％ 51％ Comparative Example 2 10％ 15％ 25％ Comparative Example 3 28％ 38％ 44％ Comparative Example 4 25％ 36％ 45％ Blank control 8％ 14％ 23％
[0131] As can be seen from the data in Table 2, compared with the comparative example and the blank control, the methods in Examples 1-3 are more conducive to the biodegradation of COP film. Under the condition of 80 days of soil landfill, the loss rate of COP film can reach up to 71%. This shows that the technical solution provided by the present invention accelerates the biodegradation of COP film by promoting the activity of microbial communities in the soil environment.
[0132] In summary, this invention, through aerobic fermentation of *Bacillus thuringiensis*, *Bacillus licheniformis*, and *Candida*, produces a fermentation product rich in enzymes and bioactive agents, which is beneficial for promoting the degradation of cyclic olefin polymers. Furthermore, by immobilizing the fermentation product with modified chitosan, microorganisms and their metabolites can be fixed on the chitosan surface, promoting the full release of active functions. In addition, the stability of the modified chitosan structure is improved through a cross-linking reaction. Therefore, the biodegradation promoter provided by this invention can not only promote the biodegradation of cyclic olefin polymers but also be used in the preparation of biodegradable cyclic olefin polymer products.
[0133] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.
Claims
1. A green synthesis method for a biodegradation promoter of cyclic olefin polymers, characterized in that, Includes the following steps: The complex original strain was subjected to aerobic fermentation and solid-liquid separation in sequence, and the liquid phase was freeze-dried to obtain the fermentation product; Hydrogen peroxide was added dropwise to a chitosan-acetic acid solution, followed by solid-liquid separation, washing, and drying to obtain modified chitosan. The modified chitosan, fermentation product, and acetic acid solution were mixed and ultrasonically dispersed to obtain an aqueous phase; The aqueous phase and the oil phase are emulsified to obtain a water-in-oil reverse microemulsion. The water-in-oil reverse microemulsion system was crosslinked, washed, and dried to obtain a cyclic olefin polymer biodegradation promoter. The compound original strains include: Chlorella vulgaris, Bacillus licheniformis and Candida albicans; The mass ratio of *Corydalis thuringiensis*, *Bacillus licheniformis*, and *Candida* in the composite strain is 1-2:2.5-3.5:0.8-1.
2. The effective viable counts of *Candida albicans*, *Bacillus licheniformis*, and *Candida* were ≥1×10⁻⁶. 8 cfu / g; The culture medium used for the aerobic fermentation uses water as a solvent and includes the following substances at the following mass concentrations: peptone 1-4 g / L, yeast extract 0.3-0.5 g / L, glucose 3-5 g / L, potassium dihydrogen phosphate 0.3-1 g / L, magnesium sulfate 0.3-1 g / L, and trehalose 5-8 g / L; the parameters for aerobic fermentation include: time 24-36 h, temperature 28-32 °C; and the mass-to-volume ratio of the compound original strain to the culture medium is 8-10 g: 50 mL. The chitosan-acetic acid solution is obtained by mixing chitosan and acetic acid solution at a mass-volume ratio of 1g:10-11mL. The acetic acid solution has a mass concentration of 1%-1.5%; The degree of deacetylation of the chitosan is 90%-95%; The volume ratio of the chitosan-acetic acid solution to hydrogen peroxide is 10-11:0.1-0.3; The volume concentration of the hydrogen peroxide is 3%-5%; The mass-to-volume ratio of the modified chitosan, fermentation product, and acetic acid solution is 1g:0.2-0.3g:10-11mL; The method for preparing the oil phase includes mixing Tween 80 and white oil at a mass ratio of 1:1.12-1.
15.
2. The green synthesis method according to claim 1, characterized in that, The parameters for ultrasonic dispersion include: power of 600-700w and time of 1-1.5h.
3. The biodegradation promoter obtained by the green synthesis method according to claim 1 or 2.
4. The use of the biodegradation promoter according to claim 3 in promoting the biodegradation of cyclic olefin polymers and / or in the preparation of products for the biodegradation of cyclic olefin polymers.