A strain capable of long-acting antagonizing poplar rot pathogen and application thereof

By isolating the endogenous azotocinus Pseudomonas Y26-15 from poplar bark and developing it into a biological control agent, the problem of unstable control effect of poplar canker disease in existing technologies has been solved. It achieves long-term antagonistic effect against poplar canker pathogens, and significantly reduces the occurrence of the disease, especially in poplars in high-altitude and cold regions.

CN120555301BActive Publication Date: 2026-05-29QINGHAI TIBET PLATEAU RES INST CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
QINGHAI TIBET PLATEAU RES INST CHINESE ACAD OF SCI
Filing Date
2025-06-26
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing microbial control agents cannot provide long-term antagonism against poplar rot pathogens, especially *Cyclocarya paliurus*, and have low geographical adaptability, resulting in unstable control effects.

Method used

A nitrogen-producing Pseudomonas strain Y26-15, which is endophytic in poplar trees, was isolated from poplar bark. It has an antagonistic effect against Chlorella vulgaris and also has growth-promoting functions such as nitrogen fixation, phosphorus solubilization, potassium solubilization, indoleacetic acid production, and siderophore production. It has been developed into a biological control agent for the prevention and control of poplar canker disease.

Benefits of technology

It significantly reduces the incidence of poplar canker and provides a long-term biological control solution. It is suitable for Beijing poplar and silver poplar in high-altitude and cold regions, and significantly reduces the incidence by 52%.

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Abstract

The application discloses a strain capable of long-acting antagonizing poplar decay pathogenic bacteria, which is Pseudomonas azotoformans, is isolated from poplar bark, and has a preservation number of CGMCC No.33365.The strain has a bacteriostatic rate of 9.68%-36.17% on live bacteria of Cytospora chrysosperma, a poplar decay pathogenic fungus, in a plate confrontation test; artificial inoculation prevention and control experiments show that the strain has a significant prevention and control effect on poplar decay; and field experiments show that the strain has a significant long-acting prevention and control effect on poplar decay.The 16s RNA gene sequence of the strain comprises the sequence shown in SEQ ID NO.1.The application also relates to application of the strain in preparation of poplar decay prevention and control products, and a composition, a bacterial agent or a biological prevention and control agent containing the strain.
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