Bacteriostatic polypeptide and application thereof in preparation of pet shower gel

Abstract

The application discloses a kind of bacteriostatic polypeptide and its preparation pet shower gel application, belong to bacteriostatic polypeptide technical field.The bacteriostatic polypeptide includes the following weight parts of raw materials: glycerol 2-5 parts, plant polypeptide 3-5 parts, sorbitol 20-40 parts, carboxymethyl cellulose 1-2 parts, xanthan gum 0.5-1 part, sodium methyl hydroxybenzoate 2-5 parts, surfactant 0.5-1 part, chlorhexidine 0.5-1 part, triclosan 0.05-0.3 part, deionized water 100 parts;The bacteriostatic polypeptide prepared in the application can be applied in the preparation process of pet shower gel, and has good bacteriostatic performance.

Description

Technical Field

[0001] The present invention relates to the technical field of antibacterial polypeptides, and in particular to an antibacterial polypeptide and an application thereof in preparing pet shower gel. Background Art

[0002] In the pet care sector, as pet owners' attention to their pets' health and hygiene continues to grow, their demands for the quality and functionality of pet bath products are also increasing. Traditional pet shampoo, primarily focused on cleansing and simple softening, no longer meets the needs of modern pet owners. From the perspective of pet skin health, pet skin differs from human skin in many ways. Pet skin has unique characteristics such as acid-base balance, barrier function, and microbial ecosystem. However, many pet shampoo products on the market often fail to fully consider these characteristics during formulation. This can lead to damage to pet skin's natural barrier, causing discomfort such as dryness and itching, and even inducing skin diseases, causing discomfort and health risks for pets.

[0003] In terms of antibacterial properties, some pet shampoo products currently contain chemically synthesized antimicrobial agents, such as certain quaternary ammonium compounds. While these can inhibit bacterial growth to a certain extent, these chemicals may be potentially toxic and irritating. Long-term use of shampoo containing these chemical antibacterial ingredients may have adverse effects on pet skin and physical health, such as allergic reactions and skin damage, and may also pollute the environment. On the other hand, many shampoo products are rich in peptides, which produce a certain amount of dark brown precipitate during storage, and the amount of precipitate increases with storage time. This precipitate formation affects the effectiveness and stability of the shampoo. Peptides are molecules with multiple biological functions, and some specific antibacterial peptides have been found to have broad-spectrum antimicrobial activity, effectively killing or inhibiting the growth of a variety of bacteria, fungi, and other microorganisms. Compared to traditional chemical antimicrobial agents, antibacterial peptides generally have higher safety, lower toxicity, and better biocompatibility. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the present invention aims to provide an antibacterial polypeptide and its use in preparing pet shower gel.

[0005] To achieve the above object, the present invention is implemented through the following technical solutions:

[0006] An antibacterial polypeptide comprises the following raw materials in parts by weight:

[0007] Glycerin 2-5 parts, plant polypeptide 3-5 parts, sorbitol 20-40 parts, sodium carboxymethyl cellulose 1-2 parts, xanthan gum 0.5-1 part, sodium methyl hydroxybenzoate 2-5 parts, surfactant 0.5-1 part, chlorhexidine 0.5-1 part, triclosan 0.05-0.3 part, deionized water 100 parts;

[0008] The surfactant is prepared by the following method:

[0009] S1: 7-octynoic acid, 14-azido-3,6,9,12-tetraoxatetradecanol reacts under the action of cuprous bromide to generate a triazole ring compound;

[0010] S2: The triazole ring compound reacts with 12-aminododecanoic acid methyl ester hydrochloride under the action of triethylamine to generate an amide compound;

[0011] S3: The amide compound reacts with 3-dimethylaminopropylamine under the action of potassium hydroxide to generate intermediate 1;

[0012] S4: Intermediate 1 reacts with 3-bromo-propyl trimethyl ammonium bromide to generate a surfactant.

[0013] The molar ratio of 7-octynoic acid to 14-azido-3,6,9,12-tetraoxatetradecanol in step S1 is 1:(1-1.2).

[0014] The molar ratio of the triazole ring compound to 12-aminododecanoic acid methyl ester hydrochloride in step S2 is 1:(1-1.2).

[0015] The molar ratio of the amide compound to 3-dimethylaminopropylamine in step S3 is 1:(1-1.5).

[0016] The molar ratio of intermediate 1 to 3-bromo-propyl trimethyl ammonium bromide in step S4 is 1:(1-1.5).

[0017] The plant polypeptide is one of corn peptide and flaxseed peptide.

[0018] The application of the bacteriostatic polypeptide in the preparation of pet shower gel.

[0019] The application mode of the bacteriostatic polypeptide in the preparation of pet shower gel is as follows: the bacteriostatic polypeptide 10-20 parts, aloe extract 10-15 parts, sea salt 3-5 parts, thickening agent 2-5 parts, disodium EDTA 1-2 parts, preservative 1-3 parts, sodium carbonate 0.5-1.5 parts, mint 0.2-0.6 parts, salicylic acid 1-3 parts, and deionized water 60 parts are weighed and mixed.

[0020] The thickening agent is one of dodecyl glucoside, cocamide propyl betaine, and coconut oil diethanolamide.

[0021] The preservative is prepared by the following method:

[0022] A1: N-dodecyl imidazole reacts with tribromoneopentyl alcohol to form a triimidazole ring compound;

[0023] A2: The triimidazole ring compound reacts with phenoxyacetic acid under the action of p-toluenesulfonic acid to generate a preservative.

[0024] The molar ratio of N-dodecyl imidazole to tribromoneopentyl alcohol in step A1 is 3:1; the molar ratio of the triimidazole ring compound to phenoxyacetic acid in step A2 is 1.5:1.

[0025] Due to the adoption of the above technical solution, the beneficial effects of the present invention include:

[0026] The antibacterial polypeptide prepared by the present invention is made of multiple ingredients, among which glycerol and sorbitol provide good solubility and water retention, plant polypeptides have antibacterial properties, sodium carboxymethyl cellulose and xanthan gum thicken and stabilize the system, and surfactants promote the mixing and penetration of ingredients. The overall antibacterial polypeptide has the advantages of good antibacterial performance and high stability. DETAILED DESCRIPTION

[0027] The present invention will be further described below with reference to the embodiments, but the present invention is not limited to these embodiments.

[0028] Example 1 Preparation of surfactant:

[0029] S1: Under sealed conditions, 200 ml of DMF, 0.1 mol of 14-azido-3,6,9,12-tetrahydrotetradecanol, and 0.1 mol of 7-octynoic acid were added to a reactor and stirred to mix. Then, 0.1 mol of cuprous bromide and 0.1 mol of N,N,N′,N′′,N′′-pentamethyldiethylenetriamine were added. The reaction was allowed to proceed at room temperature for 20 h, and then the reaction was quenched by exposing the reaction to air for 1 h. The reaction was then dialyzed against a 2 wt% EDTA solution in DMF (MwCO = 350 Da) for 24 h, and then dialyzed against deionized water (MwCO = 350 Da) for 24 h. The triazole ring compound was freeze-dried at -20°C for 12 h. The reaction equation is shown below:

[0030]

[0031] S2: Under an ice bath, 100 ml of dichloromethane, 0.1 mol of 12-aminododecanoic acid methyl ester hydrochloride, and 0.2 mol of triethylamine were added to the reactor, stirred and mixed, and then a dichloromethane solution of a triazole ring compound (0.1 mol of the triazole ring compound was dissolved in 200 ml of dichloromethane) was slowly added dropwise for 20 minutes. The mixture was warmed to room temperature and reacted for 4 hours. The mixture was washed twice with a 10 wt % sodium hydroxide solution (200 ml each time), dried with 80 g of anhydrous sodium sulfate for 1 hour, filtered, and distilled under reduced pressure at 60° C. for 4 hours. The amide compound was then separated by silica gel column chromatography. The reaction equation is shown below:

[0032]

[0033] S3: Add 300 ml of anhydrous ethanol, 0.1 mol of the amide compound, 0.1 mol of 3-dimethylaminopropylamine, and 4 g of potassium hydroxide to the reactor, stir and mix, raise the temperature to reflux for 4 h, distill under reduced pressure at 60° C. for 3 h, and dry under vacuum at 70° C. for 5 h to obtain intermediate 1; the reaction equation is as follows:

[0034]

[0035] S4: 300 ml of anhydrous ethanol, 0.1 mol of intermediate 1, and 0.1 mol of 3-bromo-propyltrimethylammonium bromide were added to the reactor, stirred and mixed, and the temperature was raised to reflux for reaction for 4 h. The mixture was distilled under reduced pressure at 60°C for 3 h. An ethanol / acetone mixed solution was added for recrystallization three times (the volume ratio of ethanol / acetone was 2:1, and 200 ml was used each time). The mixture was dried under vacuum at 70°C for 5 h to obtain a surfactant. The reaction equation is shown below:

[0036]

[0037] Its H NMR spectrum data are as follows:

[0038] 1H NMR (500 MHz, Chloroform-d) δ 7.39 (p, J = 0.9 Hz, 1H), 6.74 (t, J= 4.4 Hz, 1H), 5.85 (t, J = 4.9 Hz, 1H), 4.27 (td, J = 4.1, 0.9 Hz, 2H), 3.95(t, J = 4.2 Hz, 2H), 3.70 (s, 6H), 3.74 – 3.58 (m, 12H), 3.53 (t, J = 9.2 Hz,2H), 3.42 (t, J = 8.4 Hz, 2H), 3.27 (d, J = 16.0 Hz, 15H), 3.24 – 3.16 (m,3H), 3.12 (td, J = 5.6, 4.9 Hz, 2H), 2.75 (td, J = 8.0, 0.9 Hz, 2H), 2.19 –2.11 (m, 6H), 2.11 – 1.99 (m, 2H), 1.88 – 1.77 (m, 2H), 1.72 – 1.47 (m, 8H), 1.37 – 1.29 (m, 2H), 1.32 – 1.24 (m, 8H), 1.27 (s, 4H).

[0039] Example 2 Preparation of surfactant:

[0040] S1: Under sealed conditions, 200 ml of DMF, 0.11 mol of 14-azido-3,6,9,12-tetrahydrotetradecanol, and 0.1 mol of 7-octynoic acid were added to a reactor and stirred to mix. Then, 0.1 mol of cuprous bromide and 0.1 mol of N,N,N′,N′′,N′′-pentamethyldiethylenetriamine were added. The reaction was allowed to proceed at room temperature for 22 h, and then the reaction was quenched by exposing the reaction to air for 1 h. The reaction was then dialyzed against a 2 wt% EDTA solution in DMF (MwCO = 350 Da) for 24 h, and then dialyzed against deionized water (MwCO = 350 Da) for 24 h. The product was freeze-dried at -20°C for 12 h to obtain a triazole ring compound.

[0041] S2: Under an ice bath, 100 ml of dichloromethane, 0.11 mol of 12-aminododecanoic acid methyl ester hydrochloride, and 0.2 mol of triethylamine were added to the reactor, stirred and mixed, and then a dichloromethane solution of a triazole ring compound (0.1 mol of the triazole ring compound was dissolved in 200 ml of dichloromethane) was slowly added dropwise for 20 minutes. The mixture was warmed to room temperature and reacted for 5 hours. The mixture was washed twice with a 10 wt % sodium hydroxide solution (200 ml each time), dried with 80 g of anhydrous sodium sulfate for 1 hour, filtered, and distilled under reduced pressure at 60° C. for 4 hours. The amide compound was then separated by silica gel column chromatography;

[0042] S3: Add 300 ml of anhydrous ethanol, 0.1 mol of the amide compound, 0.12 mol of 3-dimethylaminopropylamine, and 4 g of potassium hydroxide to the reactor, stir and mix, raise the temperature to reflux for 5 h, distill under reduced pressure at 60°C for 3 h, and dry under vacuum at 70°C for 5 h to obtain intermediate 1;

[0043] S4: Add 300 ml of anhydrous ethanol, 0.1 mol of intermediate 1, and 0.12 mol of 3-bromo-propyltrimethylammonium bromide to the reactor, stir and mix, raise the temperature to reflux for 5 h, distill under reduced pressure at 60°C for 3 h, add ethanol / acetone mixed solution for recrystallization three times (the volume ratio of ethanol / acetone is 2:1, and 200 ml is used each time), and vacuum dry at 70°C for 5 h to obtain a surfactant.

[0044] Example 3 Preparation of surfactant:

[0045] S1: Under sealed conditions, 200 ml of DMF, 0.12 mol of 14-azido-3,6,9,12-tetrahydrotetradecanol, and 0.1 mol of 7-octynoic acid were added to a reactor and stirred to mix. Then, 0.1 mol of cuprous bromide and 0.1 mol of N,N,N′,N′′,N′′-pentamethyldiethylenetriamine were added. The reaction was allowed to react at room temperature for 24 h, and then the reaction was quenched by exposing the reaction to air for 1 h. The reaction was dialyzed against a 2 wt % EDTA solution in DMF (MwCO = 350 kDa) for 24 h, and then dialyzed against deionized water (MwCO = 350 Da) for 24 h. The product was freeze-dried at -20°C for 12 h to obtain a triazole ring compound.

[0046] S2: Under an ice bath, 100 ml of dichloromethane, 0.12 mol of 12-aminododecanoic acid methyl ester hydrochloride, and 0.2 mol of triethylamine were added to the reactor, stirred and mixed, and then a dichloromethane solution of a triazole ring compound (0.1 mol of the triazole ring compound was dissolved in 200 ml of dichloromethane) was slowly added dropwise for 20 minutes. The mixture was warmed to room temperature and reacted for 6 hours. The mixture was washed twice with a 10 wt % sodium hydroxide solution (200 ml each time), dried with 80 g of anhydrous sodium sulfate for 1 hour, filtered, and distilled under reduced pressure at 60° C. for 4 hours. The amide compound was then separated by silica gel column chromatography;

[0047] S3: Add 300 ml of anhydrous ethanol, 0.1 mol of the amide compound, 0.15 mol of 3-dimethylaminopropylamine, and 4 g of potassium hydroxide to the reactor, stir and mix, raise the temperature to reflux for 6 h, distill under reduced pressure at 60°C for 3 h, and dry under vacuum at 70°C for 5 h to obtain intermediate 1;

[0048] S4: Add 300 ml of anhydrous ethanol, 0.1 mol of intermediate 1, and 0.15 mol of 3-bromo-propyltrimethylammonium bromide to the reactor, stir and mix, heat to reflux for 6 h, distill under reduced pressure at 60°C for 3 h, add ethanol / acetone mixed solution for recrystallization three times (the volume ratio of ethanol / acetone is 2:1, and 200 ml is used each time), and vacuum dry at 70°C for 5 h to obtain a surfactant.

[0049] Example 4 Preparation of preservative:

[0050] A1: Add 400 ml of acetonitrile and 0.3 mol of N-dodecyl imidazole to the reaction mixture, stir and mix thoroughly, then heat to 50°C, add 0.1 mol of tribromoneopentyl alcohol, heat to reflux for 4 hours, and then distill under reduced pressure at 60°C for 4 hours to obtain a triimidazole ring compound. The reaction equation is as follows:

[0051]

[0052] A2: Under nitrogen protection, 400 g of toluene, 0.15 mol of a triimidazole ring compound, and 0.1 mol of phenoxyacetic acid were added to a reactor, stirred and mixed, and the temperature was raised to 80°C. 5 g of p-toluenesulfonic acid was then added. The reaction was allowed to proceed for 6 h (a water separator was used to remove the generated water during the reaction). The temperature was then cooled to room temperature, and a saturated sodium bicarbonate solution was slowly added to adjust the pH to neutral. The mixture was stirred thoroughly for 30 min, and allowed to stand for stratification. The organic phase was transferred to a rotary evaporator, distilled under reduced pressure at 60°C for 4 h, and dried under vacuum at 70°C for 10 h to obtain the preservative. The reaction equation is shown below:

[0053]

[0054] Its H NMR spectrum data are as follows:

[0055] 1H NMR (500 MHz, Chloroform-d) δ 10.07 (tp, J = 1.7, 0.9 Hz, 3H), 7.99 (ddt, J = 3.5, 1.7, 0.9 Hz, 3H), 7.67 (ddt, J = 3.6, 1.7, 0.9 Hz, 3H), 7.33 - 7.25 (m, 2H), 7.17 - 7.11 (m, 1H), 7.02 - 6.96 (m, 2H), 4.99 (t, J = 0.8 Hz, 6H), 4.68 (s, 2H), 4.44 (s, 2H), 4.04 (tt, J = 6.2, 0.9 Hz, 6H), 1.76 - 1.67 (m, 6H), 1.39 - 1.33 (m, 6H), 1.31 - 1.22 (m, 48H), 0.94 - 0.85 (m, 9H).

[0056] Example 5 Preparation of bacteriostatic polypeptide:

[0057] (1) glycerol 20 g, plant polypeptide (corn peptide) 30 g, sorbitol 200 g, sodium carboxymethyl cellulose 10 g, xanthan gum 5 g, sodium benzoate 20 g, surfactant (prepared in example 1) 5 g, chlorhexidine 5 g, triclosan 0.5 g, deionized water 1000 g;

[0058] (2) glycerol, sorbitol mixed and stirred for 1 h to form a uniform oil phase, under stirring, plant polypeptide, sodium carboxymethyl cellulose, xanthan gum, sodium benzoate, chlorhexidine, triclosan, deionized water were added in turn and stirred for 1 h, then surfactant was added and stirred for 0.5 h to obtain bacteriostatic polypeptide.

[0059] Example 6 Preparation of bacteriostatic polypeptide:

[0060] (1) glycerol 40 g, plant polypeptide (flaxseed peptide) 40 g, sorbitol 300 g, sodium carboxymethyl cellulose 15 g, xanthan gum 8 g, sodium benzoate 30 g, surfactant (prepared in example 2) 8 g, chlorhexidine 8 g, triclosan 2 g, deionized water 1000 g;

[0061] (2) glycerol, sorbitol mixed and stirred for 1 h to form a uniform oil phase, under stirring, plant polypeptide, sodium carboxymethyl cellulose, xanthan gum, sodium benzoate, chlorhexidine, triclosan, deionized water were added in turn and stirred for 1 h, then surfactant was added and stirred for 0.5 h to obtain bacteriostatic polypeptide.

[0062] Example 7 Preparation of bacteriostatic polypeptide:

[0063] (1) Glycerin 50g, plant polypeptide (corn peptide) 50g, sorbitol 400g, sodium carboxymethyl cellulose 20g, xanthan gum 10g, sodium methylparaben 50g, surfactant (prepared in Example 3) 10g, chlorhexidine 10g, triclosan 3g, deionized water 1000g;

[0064] (2) Glycerol and sorbitol were mixed and stirred for 1 hour to form a uniform oil phase. Plant peptides, sodium carboxymethyl cellulose, xanthan gum, sodium methylparaben, chlorhexidine, triclosan, and deionized water were added in sequence under stirring and stirred for 1 hour. Then, a surfactant was added and stirred for 0.5 hour to obtain an antibacterial peptide.

[0065] Example 8 Preparation of pet shower gel:

[0066] (1) Antibacterial polypeptide (prepared in Example 5): 100 g, aloe extract: 100 g, sea salt: 30 g, thickener (lauryl glucoside): 20 g, disodium EDTA: 10 g, preservative (prepared in Example 4): 10 g, sodium carbonate: 5 g, mint: 2 g, salicylic acid 10 g, deionized water: 600 g;

[0067] (2) Add mint and sea salt into a nano-abrasive machine and grind them until the particle size D90 is no more than 3 microns. Then put 200g of deionized water into the mixing tank, heat it to 50°C, add the antibacterial polypeptide first, stir it to make it completely suspended, add 400g of deionized water, aloe extract, sea salt, mint, salicylic acid and thickener, stir evenly; add disodium EDTA, preservatives and sodium carbonate, stir and mix evenly, and cool to room temperature to obtain pet shower gel.

[0068] Example 9 Preparation of pet shower gel:

[0069] (1) Antibacterial polypeptide (prepared in Example 6): 150 g, aloe extract: 120 g, sea salt: 40 g, thickener (cocamidopropyl betaine): 40 g, disodium EDTA: 15 g, preservative (prepared in Example 4): 20 g, sodium carbonate: 10 g, mint: 4 g, salicylic acid 20 g, deionized water: 600 g;

[0070] (2) Add mint and sea salt into a nano-abrasive machine and grind them until the particle size D90 is no more than 3 microns. Then put 200g of deionized water into the mixing tank, heat it to 50°C, add the antibacterial polypeptide first, stir it to make it completely suspended, add 400g of deionized water, aloe extract, sea salt, mint, salicylic acid and thickener, stir evenly; add disodium EDTA, preservatives and sodium carbonate, stir and mix evenly, and cool to room temperature to obtain pet shower gel.

[0071] Example 10 Preparation of pet shower gel:

[0072] (1) Antibacterial polypeptide (prepared in Example 7): 200 g, aloe extract: 150 g, sea salt: 50 g, thickener (coconut oil diethanolamide): 50 g, disodium EDTA: 20 g, preservative (prepared in Example 4): 30 g, sodium carbonate: 15 g, mint: 6 g, salicylic acid 30 g, deionized water: 600 g;

[0073] (2) Add mint and sea salt into a nano-abrasive machine and grind them until the particle size D90 is no more than 3 microns. Then put 200g of deionized water into the mixing tank, heat it to 50°C, add the antibacterial polypeptide first, stir it to make it completely suspended, add 400g of deionized water, aloe extract, sea salt, mint, salicylic acid and thickener, stir evenly; add disodium EDTA, preservatives and sodium carbonate, stir and mix evenly, and cool to room temperature to obtain pet shower gel.

[0074] Comparative Example 1

[0075] An antibacterial polypeptide, the raw material composition and process are basically the same as those in Example 6, except that the surfactant is replaced by an equal weight of the intermediate 1 prepared in step S3 of Example 2.

[0076] Comparative Example 2

[0077] An antibacterial polypeptide, the raw material composition and process are basically the same as those in Example 6, except that the surfactant is replaced by an equal weight of a surfactant prepared by the following method:

[0078] The preparation method of the surfactant is basically the same as that of Example 2, except that the 14-azido-3,6,9,12-tetrahydrotetradecanol in step S1 is replaced by an equimolar amount of 1-azidododecane.

[0079] Comparative Example 3

[0080] An antibacterial polypeptide, the raw material composition and process are basically the same as those in Example 6, except that the surfactant is replaced by an equal weight of a surfactant prepared by the following method:

[0081] The preparation method of the surfactant is basically the same as that of Example 2, except that the 12-aminododecanoic acid methyl ester hydrochloride in step S2 is replaced by an equimolar amount of 6-aminohexanoic acid methyl ester hydrochloride.

[0082] Comparative Example 4

[0083] An antibacterial polypeptide, the raw material composition and process are basically the same as those in Example 6, except that the surfactant is replaced by an equal weight of a surfactant prepared by the following method:

[0084] The preparation method of the surfactant is basically the same as that of Example 2, except that the triazole ring compound in step S2 is replaced by an equimolar amount of 2,5,8,11,14-pentaoxaheptadecan-17-oic acid.

[0085] The xanthan gum used in the examples and comparative examples of the present application is Keltrol CG with a molecular weight of 200 wDa; the molecular weight of sodium carboxymethyl cellulose is 10 wDa; the aloe extract is aloin with an effective content of 85 wt%; the sea salt is natural sea salt with a sodium chloride content of 95 wt% and a particle size of 0.5-1 mm; and the mint is mint powder with a particle size of 20-30 μm.

[0086] The antibacterial performance of the antibacterial polypeptides prepared in Examples 5-7 and Comparative Examples 1-4 was tested. The test method was based on GB / T39101-2020 "Inhibition zone method for determination of antibacterial activity of polypeptides"; the indicator standard strains were Staphylococcus aureus CICC10473 and Escherichia coli CICC10305. The test results are shown in Table 1.

[0087] Table 1 Performance indicators of antibacterial peptides

[0088]

[0089] It can be seen from Table 1 that the antibacterial polypeptides prepared in Examples 5, 6 and 7 have good antibacterial effects.

[0090] The surfactant prepared in this application contains a triazole ring (rigid structure), a long-chain alkyl group (hydrophobic segment), and a quaternary ammonium salt (hydrophilic segment), forming an amphiphilic structure that effectively encapsulates hydrophobic antimicrobial peptides, preventing their aggregation or degradation. The quaternary ammonium group targets negatively charged bacterial membranes (such as phosphatidylglycerol) through electrostatic interactions, while the triazole ring inserts into the lipid bilayer, synergistically enhancing membrane permeability. When used in combination with plant peptides, the surfactant can enhance antimicrobial efficacy.

[0091] In comparative example 1, the surfactant prepared in the present application is a diquaternary ammonium surfactant, which contains more positive charges and can be more strongly adsorbed on the bacterial surface, while the electrostatic effect of intermediate 1 is weakened and the membrane destruction efficiency is reduced.

[0092] Comparative Example 2: 1-azidododecane lacks a hydrophilic ether bond, resulting in decreased hydrophilicity and reduced adsorption efficiency on the bacterial membrane surface.

[0093] Three groups of shower gels prepared in Examples 8-10 of the present application were taken, each group with 30 mL, and kept stationary at room temperature away from light. Each group was taken out for filtration every 30 days, and the precipitate was dried with a nitrogen blower and weighed. The test results are shown in Table 2.

[0094] The raw material composition and process of the shower gel in the control group were basically the same as those in Example 9, except that the antibacterial polypeptide added was replaced by one prepared by the following method:

[0095] (1) Glycerin 40g, plant peptide (flaxseed peptide) 40g, sorbitol 300g, sodium carboxymethyl cellulose 15g, xanthan gum 8g, sodium methylparaben 30g, chlorhexidine 8g, triclosan 2g, deionized water 1000g;

[0096] (2) Glycerol and sorbitol were mixed and stirred for 1 hour to form a uniform oil phase. Plant peptides, sodium carboxymethyl cellulose, xanthan gum, sodium methylparaben, chlorhexidine, triclosan, and deionized water were added in sequence under stirring and stirred for 1 hour to obtain antibacterial peptides.

[0097] Table 2 Shower Gel Sedimentation Inhibition Performance Index

[0098]

[0099] It can be seen from Table 2 that after the surfactant is added, the amount of precipitation is about 15 mg / 30 ml after 3 months of storage, indicating that the prepared surfactant can effectively inhibit the formation of precipitation after addition.

[0100] Comparative Example 5

[0101] A pet shower gel, the raw material composition and process are basically the same as those in Example 9, except that no preservatives are added during the preparation process.

[0102] Comparative Example 6

[0103] A pet shower gel, the raw material composition and process are basically the same as those in Example 9, except that the preservative is replaced by an equal weight of a preservative prepared by the following method:

[0104] The preparation method of the preservative is basically the same as that of Example 4, except that the tribromoneopentyl alcohol in step A1 is replaced by an equal weight of 3-bromo-1-propanol.

[0105] Comparative Example 7

[0106] A pet shower gel, the raw material composition and process are basically the same as those in Example 9, except that the preservative is replaced by an equal weight of a preservative prepared by the following method:

[0107] The preparation method of the preservative is basically the same as that of Example 4, except that the phenoxyacetic acid in step A2 is replaced by an equal weight of benzoic acid.

[0108] The shower gels prepared in Examples 8-10 and Comparative Examples 5-7 of the present application were subjected to antibacterial test according to QB / T 2738-2023. The test results are shown in Table 3.

[0109] Table 3 Antibacterial performance index of shower gel

[0110]

[0111] Table 3 shows that the pet shampoo prepared in this application exhibits highly effective antibacterial properties against Escherichia coli 8099, Staphylococcus aureus ATCC6538, Candida albicans ATCC10231, and Pseudomonas aeruginosa ATCC15442. The triimidazole ring structure contained in the preservative prepared in this invention can disrupt the integrity of microbial cell membranes (through the hydrophobic N-dodecyl chain embedded in the lipid bilayer). The nitrogen atom of the imidazole ring can chelate with metal ions (calcium ions, sodium ions, etc.), blocking the activity of microbial enzymes. Furthermore, the phenoxyethyl ester group can interfere with bacterial metabolic enzymes, achieving broad-spectrum inhibition against both Gram-positive bacteria (such as Staphylococcus aureus) and Gram-negative bacteria (such as Escherichia coli).

[0112] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. However, any equivalent changes, modifications and evolutions made by ordinary technicians in this field without departing from the scope of the technical solution of the present invention by using the technical content disclosed above are all equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of protection of the technical solution of the present invention.

Claims

1. An antibacterial polypeptide, characterized in that The composition comprises the following raw materials in parts by weight: 2-5 parts of glycerin, 3-5 parts of plant peptides, 20-40 parts of sorbitol, 1-2 parts of sodium carboxymethyl cellulose, 0.5-1 part of xanthan gum, 2-5 parts of sodium methylparaben, 0.5-1 part of surfactant, 0.5-1 part of chlorhexidine, 0.05-0.3 part of triclosan, 100 parts of deionized water; The surfactant is prepared by the following method: S1: 7-octynoic acid and 14-azido-3,6,9,12-tetrahydrotetradecanol react in the presence of cuprous bromide to form a triazole ring compound; S2: The triazole ring compound reacts with 12-aminododecanoic acid methyl ester hydrochloride in the presence of triethylamine to form an amide compound; S3: The amide compound reacts with 3-dimethylaminopropylamine in the presence of potassium hydroxide to form intermediate 1; S4: Intermediate 1 reacts with 3-bromo-propyltrimethylammonium bromide to form a surfactant; The plant polypeptide is one of corn peptide and flaxseed peptide.

2. The antibacterial polypeptide according to claim 1, characterized in that In step S1, the molar ratio of 7-octynoic acid to 14-azido-3,6,9,12-tetrahydrotetradecanol is 1:(1-1.2).

3. The antibacterial polypeptide according to claim 1, characterized in that In step S2, the molar ratio of the triazole ring compound to 12-aminododecanoic acid methyl ester hydrochloride is 1:(1-1.2).

4. The antibacterial polypeptide according to claim 1, characterized in that The molar ratio of the amide compound to 3-dimethylaminopropylamine in step S3 is 1:(1-1.5); the molar ratio of the intermediate 1 to 3-bromo-propyltrimethylammonium bromide in step S4 is 1:(1-1.5).

5. Use of the antibacterial polypeptide according to any one of claims 1 to 4 in the preparation of pet shower gel.

6. The use according to claim 5, characterized in that The application method is as follows: 10-20 parts of the antibacterial polypeptide according to any one of claims 1 to 4, 10-15 parts of aloe extract, 3-5 parts of sea salt, 2-5 parts of thickener, 1-2 parts of disodium EDTA, 1-3 parts of preservative, 0.5-1.5 parts of sodium carbonate, 0.2-0.6 parts of mint, 1-3 parts of salicylic acid, and 60 parts of deionized water are stirred and mixed by weight.

7. The use according to claim 6, characterized in that The thickener is one of lauryl glucoside, cocamidopropyl betaine and coconut oil diethanolamide.

8. The use according to claim 6, characterized in that The preservative is prepared by the following method: A1: N-dodecyl imidazole reacts with tribromoneopentyl alcohol to form a triimidazole ring compound; A2: The triimidazole ring compound reacts with phenoxyacetic acid under the action of p-toluenesulfonic acid to generate a preservative.

9. The use according to claim 8, characterized in that The molar ratio of N-dodecyl imidazole to tribromoneopentyl alcohol in step A1 is 3:1; the molar ratio of the triimidazole ring compound to phenoxyacetic acid in step A2 is 1.5:1.

Images