Multi-antigen epitope peptide recombinant protein of three lethal toxins of clostridium perfringens as well as design method and application of multi-antigen epitope peptide recombinant protein

By designing a multi-antigen epitope peptide recombinant protein that produces three lethal toxins of Clostridium perfringens, the safety and immunogenicity problems in the production process of existing vaccines are solved, and efficient vaccine protection effects are achieved.

CN120574331AActive Publication Date: 2025-09-02NINGXIA UNIVERSITY
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Patent Information

Application Number
CN202510669594.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-23
Publication Date
2025-09-02
Estimated Expiration
2045-05-23

AI Technical Summary

Technical Problem

There are problems such as biosafety risks, strain degeneration, inconsistent immunogenicity, formaldehyde residue, unknown allergenicity in the production process of existing Clostridium perfringens toxin vaccines, and recombinant protein vaccines have problems such as large workload, large molecular weight of expression fusion proteins, and conformational changes.

Method used

A multi-antigen epitope peptide recombinant protein with three lethal toxins of Clostridium perfringens was designed, and antigen epitopes of B, Tc and Th cells were screened through immunoinformatics and structural vaccines, and a multi-antigen epitope peptide recombinant protein was constructed, and expressed and purified in E. coli, ensuring that it is non-toxic, non-sensitizing, and high antigenicity, and can be recognized by TLR-4.

Benefits of technology

It has achieved efficient induction of humoral and cellular immune responses, protected the immune mice to produce 100% neutralizing antibodies, successfully protected more than half of the mice's immune effects, and provided efficient and safe vaccine candidates.

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Abstract

The invention is applicable to the technical field of immunology, and provides a multi-antigen epitope peptide recombinant protein of three lethal toxins of clostridium perfringens as well as a design method and application thereof. Composition fragments of the multi-antigen epitope peptide recombinant protein comprise B lymphocyte antigen epitopes, cytotoxic T lymphocyte antigen epitopes and helper T lymphocyte antigen epitopes of three lethal toxin proteins, and the antigen epitopes are combined through KK, GPGPG and AAY joints; the three types of lethal toxin proteins are Alpha, Beta1 and Beta2; the amino acid sequence of the multi-antigen epitope peptide recombinant protein is shown as SEQ ID NO. 1 in a sequence table. According to the present invention, three toxin proteins are adopted as target spots, peptide fragments with poor antigenicity, potential toxicity and sensitization are removed, and a plurality of antigen epitope peptides are integrated into a recombinant protein to be adopted as an immunogen so as to prepare the efficient, safe and broad-spectrum vaccine, and the vaccine has excellent biological safety in animal bodies, and can be widely used in animal bodies. The clostridium perfringens toxin vaccine has huge potential to replace the traditional clostridium perfringens toxin vaccine.
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