A miticidal composition and use thereof

Abstract

The application belongs to the technical field of pesticide mite-killing, and discloses a mite-killing composition and application thereof, wherein the mite-killing composition comprises active ingredient A and active ingredient B; the active ingredient A is a compound shown in formula I; and the active ingredient B is selected from any one of a mitochondrial respiration inhibitor mite-killing agent, a growth inhibitor mite-killing agent, a neurotoxin mite-killing agent and other mite-killing agents. The mite-killing composition has obvious synergistic effect, has good control effect on common leaf mite family harmful mites of crops, can effectively control the harm of mite pests, reduces the use dose of pesticides and reduces the cost of agricultural production.

Description

Technical Field

[0001] This invention belongs to the field of pesticide acaricide technology, and discloses an acaricide composition and its application. Background Technology

[0002] Agricultural mites are important harmful arthropods affecting crops, fruit trees, vegetables, forest trees, and flowers. They are characterized by their tiny size, wide range of activity, rapid reproduction, short generation cycle, strong adaptability, and strong resistance to pesticides. Due to the extensive use of single chemical pesticides, agricultural mites have risen from minor pests to major pests, and have spread throughout my country, becoming a significant group of harmful organisms. The damage caused by agricultural mites has driven the development of acaricides, but the widespread use of acaricides has led to pesticide resistance in mites, constantly creating new demands for pesticides.

[0003] Given the increasingly serious harm caused by mites, the use of pesticides to control mite infestations remains an important means of prevention and control. However, the development of pesticide resistance in mites, the limited variety of new acaricides, and the lagging technological updates in the application of acaricides have led to the overuse of pesticides, increased pollution, and increasing difficulty in controlling mites. Therefore, there is an urgent need for highly efficient, safe, and environmentally friendly acaricides in agricultural production. The inventors of this application have developed an environmentally friendly acaricide with significant synergistic effects by rationally mixing the compound of Formula I with other acaricides, which can effectively delay the development of pesticide resistance in mites. Summary of the Invention

[0004] Based on the above, the purpose of this invention is to provide an acaricide composition and its application. The acaricide composition contains a structure of formula I and another acaricide, which can be used to control harmful mites on crops, fruit trees, vegetables, forest trees and flowers. The acaricide has a significant synergistic effect on harmful mites, is environmentally safe, can effectively slow down the development and growth of pesticide resistance in mites, reduce the amount of pesticides used, and thus reduce agricultural production costs.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: an acaricidal composition, wherein the acaricidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula I: The active ingredient B is selected from any of the following acaricides: mitochondrial respiration inhibitors, growth inhibitors, neurotoxins, and other acaricides.

[0006] Furthermore, the active ingredient A is a compound represented by Formula I: The active ingredient B is selected from mitochondrial respiration inhibitors: pyridaben, acetamiprid, vaniliprole, fipronil, quinacrine, chlorfenapyr, bromonitrile, difenoconazole, fenpyroximate, pyridaben, pyrimethanil, pyflubumide, nicofluprole, fometoquin, quinacrine, pyrimethanil, flufenoxuron, pyrimethanil, chlorfenapyr, bromonitrile, pyrimethanil, diflubenzuron, fluazinam, pyridaben;

[0007] Growth inhibitor acaricides: Tetramethrin, Flufenoxam, Spirotetramethrin, Spirodiclofen, Spirodiclofen, Spirodiclofen diester, Tifenoxam, Tifenoxam, Etoxazole, Fluxametamide, Isocycloseram, Cyclopyralid, Lufenuron, Flufenoxuron, Flufenoxuron, Thiamethoxam;

[0008] Neurotoxic acaricides: abamectin, methyl abamectin benzoate, liuyangmycin, huaguangmycin, mebifenthin, bifenazate, high-efficiency cypermethrin, deltamethrin, cypermethrin, bifenthrin, flufenoxuron, bromofenoxuron, triazole tin, fenbutatin, acynonapyr;

[0009] Other acaricides: any one of the following: propargite, flupentiofenox, amidoflumet, matrine, veratrine, azadirachtin, pyrethroids, rotenone, pine miticide, olive miticide, mivorilaner, modoflaner, tigolaner, umifoxolaner, and trifluralin.

[0010] Furthermore, the active ingredient B is selected from any one of the following: abamectin, spirodiclofen, bifenazate, etoxazole, difenoconazole, abamectin, emamectin benzoate, propargite, pyridaben, lambda-cyhalothrin, etoxazole, diflubenzuron, cyprodinil, lufenuron, fluazinam, spirodiclofen, tetradifon, thiamethoxam, spirotetramat, or cypermethrin;

[0011] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:50 to 60:1, or any value within the above range.

[0012] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1, or any value within the above range.

[0013] Further, the active ingredient B is azoxystrobin, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 25:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:14 to 25:1, or any value within the above range.

[0014] Alternatively, the active ingredient B is spirodiclofen, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 25:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:8 to 16:1, or any value within the above range.

[0015] Alternatively, the active ingredient B is biphenylhydrazine ester, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 16:1, or any value within the above range.

[0016] Alternatively, the active ingredient B is etoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 40:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1, or any value within the above range.

[0017] Alternatively, the active ingredient B is butyl ether urea, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 30:1, or any value within the above range.

[0018] Alternatively, the active ingredient B is avermectin, and the mass ratio of active ingredient A to active ingredient B is 1:15 to 50:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:8 to 40:1, or any value within the above range.

[0019] Alternatively, the active ingredient B is emamectin benzoate, and the mass ratio of active ingredient A to active ingredient B is 1:12 to 45:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:5 to 35:1, or any value within the above range.

[0020] Alternatively, the active ingredient B is propargite, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 20:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:30 to 10:1, or any value within the above range.

[0021] Alternatively, the active ingredient B is pyridaben, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 35:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:10 to 35:1, or any value within the above range.

[0022] Alternatively, the active ingredient B is high-efficiency cyhalothrin, and the mass ratio of active ingredient A to active ingredient B is 1:32 to 38:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:12 to 24:1, or any value within the above range.

[0023] Alternatively, the active ingredient B is etoxazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 44:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 30:1, or any value within the above range.

[0024] Alternatively, the active ingredient B is dicofol, and the mass ratio of active ingredient A to active ingredient B is 1:28 to 42:1. Preferably, the mass ratio of active ingredient A to active ingredient B is 1:14 to 42:1, or any value within the above range.

[0025] Alternatively, the active ingredient B is pyridaben, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 40:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 20:1, or any value within the above range.

[0026] Alternatively, the active ingredient B is lufenuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 40:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 30:1, or any value within the above range.

[0027] Alternatively, the active ingredient B is fluazinam, and the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 32:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:48 to 12:1, or any value within the above range.

[0028] Alternatively, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 35:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 35:1, or any value within the above range.

[0029] Alternatively, the active ingredient B is tetradifon, and the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 24:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 16:1, or any value within the above range.

[0030] Alternatively, the active ingredient B is thiamethoxam, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:26 to 30:1, or any value within the above range.

[0031] Alternatively, the active ingredient B is spirotetramat, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 35:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 10:1, or any value within the above range.

[0032] Alternatively, the active ingredient B is cypermethrin, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:1. Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1, or any value within the above range.

[0033] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:25 to 30:1, or any value within the above range.

[0034] Furthermore, the total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 90% of the total weight of the acaricide composition.

[0035] Furthermore, based on a total weight of 100 wt%, the total weight of active ingredient A and active ingredient B accounts for 2% to 80% of the total weight of the acaricidal composition.

[0036] Furthermore, the acaricidal composition can be prepared into an agriculturally acceptable dosage form, wherein the dosage form is a liquid formulation and / or a solid formulation. The liquid formulation is selected from soluble concentrates, soluble gels, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspension emulsions, microcapsule suspension-suspension, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. The solid formulation is selected from powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0037] Furthermore, the liquid formulation is selected from soluble concentrates, emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions, or suspensions, and the solid formulation is selected from powders, granules, wettable powders, or water-dispersible granules.

[0038] The present invention also discloses the use of the acaricidal composition described above for the control of plant mites.

[0039] Furthermore, the plants mentioned are crops, fruit trees, vegetables, forest trees and flowers, and the mites mentioned are carmine spider mites, two-spotted spider mites, citrus spider mites, truncated spider mites and Kanzawa spider mites.

[0040] Furthermore, the aforementioned spider mites are Tetranychus carmineus, Tetranychus two-spotted, or Tetranychus truncatula.

[0041] The beneficial effects of this invention are:

[0042] 1) The acaricide composition of the present invention has a significant synergistic effect on plant mites, and can effectively control the damage of mites to plants. It can be applied to the prevention and control of mites in crops, fruit trees, vegetables, forest trees and flowers.

[0043] 2) The acaricide composition of the present invention can preferentially delay the emergence and development of acaricide resistance in mites, is safe for their natural enemies, is safe for crops, and leaves little environmental residue. Detailed Implementation

[0044] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0045] Formulation preparation examples and methods:

[0046] Preparation method of water-dispersible granules: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added thereto. After mixing, the mixture is pulverized by air jet and 10-25% water is added. Then, the mixture is kneaded, granulated, dried and sieved to obtain the water-dispersible granule product; or the pulverized powder is sprayed with water, granulated and dried in a fluidized bed granulator, and then sieved to obtain the water-dispersible granules of the present invention.

[0047] Preparation method of water-in-oil emulsion: According to the formulation ratio of the preparation example, dissolve the active ingredient in the solvent and add the emulsifier to dissolve it into a uniform oil phase. Mix the deionized water and antifreeze together to form a uniform aqueous phase. Under high-speed shearing, add the aqueous phase to the oil phase to form a well-dispersed water-in-oil emulsion product.

[0048] Emulsifiable concentrate preparation method: According to the formula ratio, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable concentrate of the present invention.

[0049] Microemulsion preparation method: According to the formula ratio, the active ingredients, solvents, emulsifiers, etc. are mixed evenly to obtain an oil phase. The antifreeze and water are mixed evenly to obtain an aqueous phase. The oil phase is added to the aqueous phase under stirring and stirred evenly. Shearing is continued for 10 minutes. Then, silicone oil defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01 to 0.1 micrometers, which is the microemulsion described in this invention.

[0050] Preparation method of wettable powder: According to the formulation ratio of the preparation example, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a stirring tank. The mixture is then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0051] Suspension preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0052]

[0053]

[0054] Indoor activity test:

[0055] Example 1: Indoor toxicity test and compound ratio screening. The compound of formula I was rationally compounded with other acaricides to determine its activity against Tetranychus truncatula.

[0056] Experimental targets: adult and nymphal Tetranychus truncatus Ehara.

[0057] The indoor test was conducted in accordance with NY / T 1154.13-2008 "Guidelines for Indoor Bioassay of Pesticides - Insecticides - Part 13: Leaf Disc Spraying Method". Uniformly growing broad bean leaves were selected, and suitable leaf discs were made using a perforator. A damp sponge was placed in the petri dish, filter paper was placed on the sponge, and leaf discs were placed on the filter paper, with two leaf discs per dish. Target mites were attached to the leaf discs, with 15 mites per leaf disc.

[0058] The test active ingredient was prepared into a stock solution using an organic solvent, and then five series of concentrations were prepared using a 0.1% Tween 80 aqueous solution in equal proportions.

[0059] Adjust the Potter spray tower pressure to 1.47 × 10⁻⁶. 5To ensure the stability of Pa, the cleaned spray head was first rinsed twice with acetone, then twice with distilled water. The culture dish was placed in the bottom tray of the spray tower for spraying, with a spray volume of 1 mL. After the solution settled for 1 minute, the treated insects were removed and transferred to suitable conditions for rearing and observation. Each treatment was repeated four times, and a control group containing no pesticide (containing all organic solvents and emulsifiers) was included. Forty-eight hours after treatment, the mortality of the insects was checked, and the total number of insects and the number of dead insects were recorded.

[0060] Data statistics and analysis:

[0061] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0062]

[0063] In the formula:

[0064] P – Mortality rate, expressed as a percentage (%);

[0065] K represents the number of dead insects, in heads;

[0066] N represents the total number of insects treated, in units of heads.

[0067]

[0068] In the formula:

[0069] P1 – Corrected mortality rate, in percentage (%);

[0070] P t —The mortality rate is expressed as a percentage (%).

[0071] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0072] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0073] The experiment was analyzed using the DPS statistical analysis system to determine the toxicity regression equation, correlation coefficient, and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0074] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0075]

[0076] In the formula:

[0077] ATI – Actual Measured Toxicity Index of Mixtures;

[0078] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0079] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0080] TTI = TI A ×P A +TI B ×P B

[0081] In the formula:

[0082] TTI – Theoretical Toxicity Index of Mixtures;

[0083] TI A —A. Toxicity index of drug A;

[0084] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0085] TI B —Toxicity index of drug B;

[0086] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0087]

[0088] In the formula:

[0089] CTC – Cotoxicity Coefficient;

[0090] ATI – Actual Measured Toxicity Index of Mixtures;

[0091] TTI – Theoretical Toxicity Index of Mixtures.

[0092] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0093] Table 1. Results of indoor activity assays of compound I mixed with azithromycin on adult Tetranychus truncatula.

[0094]

[0095] The experimental results (see Table 1) show that the mass ratio of compound I to abamectin in the range of 1:20 to 25:1 has a good synergistic effect on adult spider mites. Among them, the co-toxicity coefficient of compound I to abamectin in the range of 1:14 to 25:1 is greater than 130, and the synergistic effect is significant.

[0096] Table 2 shows the results of indoor activity assays of compound I mixed with spirodiclofen against adult Tetranychus truncatula.

[0097]

[0098]

[0099] The experimental results (see Table 2) show that the mixture of compound I and spirodiclofen in the range of 1:20 to 25:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, when the mass ratio of compound I to spirodiclofen is in the range of 1:8 to 16:1, the co-toxicity coefficient of adult Tetranychus truncatula is greater than 130, indicating a significant synergistic effect.

[0100] Table 3. Results of indoor activity assays of compound I mixed with biphenylhydrazine on adult Tetranychus truncatula.

[0101]

[0102] The experimental results (see Table 3) show that the mass ratio of compound I to biphenylhydrazine in the range of 1:25 to 30:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to biphenylhydrazine in the range of 1:25 to 16:1 is greater than 130, and the synergistic effect is significant.

[0103] Table 4. Results of indoor activity assays of compound I mixed with etoxazole and adult Tetranychus truncatula.

[0104]

[0105]

[0106] The experimental results (see Table 4) show that the mass ratio of compound I to etoxazole in the range of 1:20 to 40:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to etoxazole in the range of 1:10 to 20:1 is greater than 130, indicating a significant synergistic effect.

[0107] Table 5. Results of indoor activity assays of compound I mixed with bufenozide on adult Tetranychus truncatula.

[0108]

[0109] The experimental results (see Table 5) show that the mass ratio of compound I to butylated urea in the range of 1:25 to 30:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to butylated urea in the range of 1:15 to 20:1 is greater than 130 for adult Tetranychus truncatula, indicating a significant synergistic effect.

[0110] Table 6. Results of indoor activity assays of compound I mixed with avermectin on adult Tetranychus truncatula.

[0111]

[0112] The experimental results (see Table 6) show that the mass ratio of compound I to abamectin in the range of 1:15 to 50:1 exhibits a good synergistic effect on adult spider mites. Among them, when the mass ratio of compound I to abamectin is in the range of 1:8 to 40:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect.

[0113] Table 7. Results of indoor activity assays of compound I mixed with emamectin benzoate on adult Tetranychus truncatula.

[0114]

[0115] The experimental results (see Table 7) show that the mass ratio of compound I to emamectin benzoate in the mixture ranges from 1:12 to 45:1, exhibits a good synergistic effect on adult spider mites. Among them, when the mass ratio of compound I to emamectin benzoate is in the range of 1:5 to 35:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect.

[0116] Table 8. Results of indoor activity assays of compound I with specific mixtures of *Tetranychus truncatus* and *Arthraceae* mites.

[0117]

[0118] The experimental results (see Table 8) show that the mass ratio of compound I to propargite in the mixture is 1:30 to 20:1, which has a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to propargite in the mass ratio is greater than 130 in the range of 1:30 to 10:1, indicating a significant synergistic effect.

[0119] Table 9. Results of indoor activity assays of compound I mixed with pyridaben on adult Tetranychus truncatula.

[0120]

[0121] The experimental results (see Table 9) show that the mass ratio of compound I to pyridaben in the range of 1:20 to 35:1 exhibits a good synergistic effect on adult spider mites. Among them, the co-toxicity coefficient of compound I to pyridaben is greater than 130 in the mass ratio range of 1:10 to 35:1, indicating a significant synergistic effect.

[0122] Table 10 Results of Indoor Activity Determination Tests on Adult Tetranychus truncatulae Using Compound I Mixed with High-Efficiency Cyfluthrin

[0123] Test reagents virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Formula I compound y = 2.7316 + 1.5221x 0.9954 30.9248 / High-efficiency cyhalothrin y = 3.9648 + 1.5698x 0.9998 4.5652 / 1:32 y = 4.1140 + 1.5116x 0.9986 3.8558 121.538 1:12 y = 4.2193 + 1.4449x 0.9990 3.4696 140.810 1:6 y = 4.3274 + 1.3244x 0.9973 3.2201 161.429 5:1 y = 3.6147 + 1.4532x 0.9962 8.9792 175.507 10:1 y = 3.6340 + 1.2320x 0.9960 12.8446 157.885 24:1 y = 3.2479 + 1.4211x 0.9994 17.0958 146.951 38:1 y = 3.2961 + 1.2786x 0.9989 21.5074 125.244

[0124] The experimental results (see Table 10) show that the compound of formula I mixed with lambda-cyhalothrin in a mass ratio of 1:32 to 38:1 exhibits a good synergistic effect on adult spider mites. Among them, when the mass ratio of compound I to lambda-cyhalothrin is in the range of 1:12 to 24:1, the co-toxicity coefficient is greater than 130, and the synergistic effect is significant.

[0125] Table 11 Results of indoor activity assays of compound I mixed with etoxazole on adult Tetranychus truncatula.

[0126]

[0127]

[0128] The experimental results (see Table 11) show that the mass ratio of compound I to etoxazole in the range of 1:24 to 44:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to etoxazole in the range of 1:24 to 30:1 is greater than 130, indicating a significant synergistic effect.

[0129] Table 12 Results of indoor activity assays of compound I mixed with fenfluridine on adult Tetranychus truncatula.

[0130]

[0131] The experimental results (see Table 12) show that the mass ratio of compound I to tebufenozide in the range of 1:28 to 42:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to tebufenozide in the range of 1:14 to 42:1 is greater than 130, indicating a significant synergistic effect.

[0132] Table 13 Results of indoor activity assays of compound I mixed with cyproterone against adult Tetranychus truncatula.

[0133]

[0134] The experimental results (see Table 13) show that the mass ratio of compound I to cyproterone in the mixture is 1:30 to 40:1, which has a good synergistic effect on adult Tetranychus truncatula. Among them, when the mass ratio of compound I to cyproterone is in the range of 1:10 to 20:1, the co-toxicity coefficient is greater than 130, and the synergistic effect is significant.

[0135] Table 14 Results of Indoor Activity Assays of Compound I and Lufenuron Mixed with Adult Tetranychus truncatulae

[0136]

[0137] The experimental results (see Table 14) show that the mass ratio of compound I to lufenuron in the range of 1:25 to 40:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to lufenuron in the range of 1:15 to 30:1 is greater than 130, indicating a significant synergistic effect.

[0138] Table 15 Results of indoor activity assays of compound I mixed with fluazinam against adult Tetranychus truncatula.

[0139]

[0140] The experimental results (see Table 15) show that the mass ratio of compound I to fluazinam in the range of 1:48 to 32:1 exhibits a good synergistic effect on adult Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to fluazinam is greater than 130 when the mass ratio is 1:48 to 12:1, indicating a significant synergistic effect.

[0141] Table 16 Results of indoor activity assays of compound I mixed with spirodiclofen against Tetranychus truncatula nymphs

[0142]

[0143] The experimental results (see Table 16) show that the mass ratio of compound I to spirodiclofen in the range of 1:40 to 35:1 exhibits a good synergistic effect on Tetranychus truncatula nymphs. Among them, when the mass ratio of compound I to spirodiclofen is in the range of 1:25 to 35:1, the co-toxicity coefficient is greater than 130, and the synergistic effect is significant.

[0144] Table 17 Results of indoor activity assays of compound I mixed with tetradifon on Tetranychus truncatula nymphs.

[0145]

[0146] The experimental results (see Table 17) show that the mass ratio of compound I to tetradifon in the mixture is 1:24 to 24:1, which has a good synergistic effect on the nymphs of Tetranychus truncatula. Among them, the co-toxicity coefficient of compound I to tetradifon is greater than 130 when the mass ratio is 1:24 to 16:1, indicating a significant synergistic effect.

[0147] Table 18 Results of indoor activity assays of compound I mixed with thiamethoxam against Tetranychus truncatula nymphs

[0148]

[0149]

[0150] The experimental results (see Table 18) show that the mass ratio of compound I to thiamethoxam in the range of 1:35 to 30:1 exhibits a good synergistic effect on Tetranychus truncatula nymphs. Among them, the co-toxicity coefficient of compound I to thiamethoxam in the range of 1:26 to 30:1 is greater than 130, indicating a significant synergistic effect.

[0151] Table 19 Results of indoor activity assays of compound I mixed with spirotetramatid nymphs on Tetranychus truncatula.

[0152]

[0153] The experimental results (see Table 19) show that the mass ratio of compound I to spirotetramatine in the range of 1:30 to 35:1 exhibits a good synergistic effect on Tetranychus truncatula nymphs. Among them, the co-toxicity coefficient of compound I to spirotetramatine in the mass ratio range of 1:26 to 30:1 is greater than 130, indicating a significant synergistic effect.

[0154] Table 20 shows the results of indoor activity assays of compound I mixed with cypermethrin on nymphs of Tetranychus truncatula.

[0155]

[0156]

[0157] The experimental results (see Table 20) show that the mass ratio of compound I to cypermethrin in the range of 1:35 to 30:1 exhibits a good synergistic effect on Tetranychus truncatula nymphs. Among them, the co-toxicity coefficient of compound I to cypermethrin in the range of 1:26 to 30:1 is greater than 130, indicating a significant synergistic effect.

[0158] This invention employs the research method described above, and rationally combines the compound of Formula I with any one of the following acaricides: fenpropathrin, spirodiclofen, trifluralin, pyrimethanil, flufenoxuron, diflubenzuron, flufenoxuron, sulfadiazine, acetamiprid, pyrimethanil, fenpropathrin, chlorfenapyr (bromnifenoxuron), benzylfenoxuron, pyrimethanil, micamycin, fipronil, pyridaben, quinfenoxuron, bromodiphenyl ether, ethyl ester dicofol, chlorpyrifos, triazophos, amitraz, fenbutatin, amitraz hydrochloride, flupentiofenox, acynonapyr, pyflubumide, and vaniliprole. The compound of Formula I exhibits a synergistic effect against Tetranychus truncatula when the mass ratio of the above acaricides is within the range of 1:15 to 20:1.

[0159] Field efficacy trials:

[0160] Example 2: Field control trials of different acaricide compositions against maize red spider mites

[0161] The experiment was conducted in a cornfield in Jiaxiang County, Jining City, Shandong Province. The experimental field experienced a complex infestation of corn spider mites, with the main damaging species being *Tetranychus truncatus* and *Tetranychus carmine*. No other insecticides were used during the experiment, and the surrounding crops were all corn. The experiment consisted of 23 treatments, each replicated four times, arranged in a randomized block design, with each plot measuring 30 m². 2 The experiment was conducted during the peak period of corn spider mite infestation. Mite population density was investigated before application and at 3, 7, and 15 days after application. Five corn plants were surveyed in each plot, and one leaf from each plant (top, middle, and bottom) was surveyed, for a total of 15 leaves. The corn leaves were examined with a handheld magnifying glass, and the number of active mites was recorded. The control effect was calculated.

[0162] The prevention and control effect is calculated using the following formula:

[0163]

[0164] The field control effects of different treatments on maize red spider mites were tested:

[0165] Table 21 Results of field trials on red spider mites in corn using different acaricide compositions.

[0166]

[0167]

[0168] During the field trials, irregular surveys were conducted, and no effects were found on the corn plants or their growth from the various acaricide treatments.

[0169] Table 21 shows the results of field trials of different tested pesticides against corn spider mites. It can be seen that the mixture of compound I with any one of the following pesticides—bufenozide, abamectin, bifenazate, emamectin benzoate, abamectin, spirodiclofen, pyridaben, etoxazole, propargite, tetradifon, and fluazinam—exhibited better control effects against corn spider mites, with significantly higher efficacy than the single control pesticide.

[0170] Example 3: Field trial of different acaricide compositions for the control of eggplant spider mites

[0171] Experimental Site: The experiment was conducted in Beihe Village, Yaozhuang Town, Jiaxing City, Zhejiang Province, using greenhouse cultivation. The experimental site has flat terrain and moderate soil fertility, conforming to local scientific agricultural practices.

[0172] Experimental crops and targets: eggplant, eggplant spider mites (mainly Tetranychus carmineus and Tetranychus tinctoria).

[0173] Test reagents: The test reagents and control reagents are shown in Table 22.

[0174] Experimental plot arrangement: The experimental, control, and blank control plots were randomly arranged, with each plot measuring 10 m². 2 Each treatment was repeated 4 times.

[0175] Experimental Methods: During the peak occurrence period of eggplant spider mites, the entire eggplant plant was sprayed according to the experimental design. A Dongfanghong DFH-16A manual sprayer was used to spray the entire eggplant plant once at a rate of 500 L / hm². 2 No other pesticides were used during the trial period in any of the plots.

[0176] Experimental survey: The control effect was investigated on the 3rd and 14th days after application of the pesticide. During the survey, 50 leaves were collected from each plot to count the number of surviving mites and nymphs.

[0177] Safety survey: During the experiment, the height of eggplant plants, leaf color and any adverse effects on other organisms were observed by visual inspection.

[0178] The prevention and control effect is calculated using the following formula:

[0179]

[0180]

[0181] The test results are shown in the table below:

[0182] Table 22 Results of field trials on eggplant spider mite control using different acaricide compositions.

[0183]

[0184] During the field trials, irregular surveys were conducted, and no effects were found on the eggplant plants or their growth from the various acaricide treatments.

[0185] As shown in Table 22, the results of field trials of different tested pesticides against eggplant spider mites indicate that the mixture of compound I with any one of etoxazole, thiamethoxam, cypermethrin, lufenuron, spirodiclofen, lambda-cyhalothrin, cypermethrin, diflubenzuron, and spirotetramat showed good control effects against eggplant spider mites.

[0186] Through indoor toxicity testing and field efficacy trials on crops such as corn and eggplant, the compound of Formula I and the acaricide composition described in this invention, when rationally combined, exhibit excellent control effects against mites. The acaricide composition or its formulation obtained by this invention demonstrates significant efficacy, superior to single agents in delaying the development of resistance and prolonging pesticide retention. Furthermore, no phytotoxicity was observed in the field efficacy trials, indicating that the enhanced synergistic effect of the acaricide composition or formulation reduces production and usage costs while ensuring crop safety.

[0187] It should be understood that the above embodiments are merely some embodiments of the present invention, provided only to better understand the embodiments of the present invention, and are not all embodiments of the present invention. In practical applications, by adjusting the content of each component and the composition of the components in the present invention, different and numerous embodiments can be obtained, all of which are within the scope of the present invention.

[0188] The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiments. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A mite-killing composition, characterized in that, The acaricide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula I: (I) The active ingredient B is selected from avermectin and emamectin benzoate; the active ingredient B is avermectin, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 50:1; the active ingredient B is emamectin benzoate, and the mass ratio of the active ingredient A to the active ingredient B is 1:12 to 45:

1.

2. The acaricide composition according to claim 1, characterized in that, The active ingredient B is avermectin, and the mass ratio of active ingredient A to active ingredient B is 1:8 to 40:1; the active ingredient B is emamectin benzoate, and the mass ratio of active ingredient A to active ingredient B is 1:5 to 35:

1.

3. The acaricide composition according to claim 1, characterized in that, The active ingredient B is avermectin, and the mass ratio of active ingredient A to active ingredient B is 1:15, 1:8, 5:1, 10:1, 20:1, 40:1, or 50:1; the active ingredient B is emamectin benzoate, and the mass ratio of active ingredient A to active ingredient B is 1:12, 1:5, 6:1, 10:1, 15:1, 25:1, 35:1, or 45:

1.

4. The acaricide composition according to claim 1, characterized in that, The total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5% to 90% of the total weight of the acaricide composition.

5. The acaricide composition according to claim 4, characterized in that, The total weight of the acaricide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 2% to 80% of the total weight of the acaricide composition.

6. The acaricide composition according to claim 1, characterized in that, The acaricide composition is prepared into an agriculturally acceptable formulation, wherein the formulation is a liquid or solid formulation, wherein the liquid formulation is selected from soluble concentrates, emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions or suspensions, and the solid formulation is selected from powders, granules, wettable powders or water-dispersible granules.

7. The use of the acaricidal composition according to any one of claims 1-6 for the control of plant mites, characterized in that, The pest mentioned is the truncated spider mite.

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