Fishing vaccine continuous soaking inoculation method based on Archimedes screw principle

Abstract

The invention discloses a fishing vaccine continuous soaking inoculation method based on the Archimedes screw principle. The fishing vaccine continuous soaking inoculation method comprises the steps that fish schools and vaccine soaking liquid are synchronously conveyed to a cavity formed between a soaking cylinder and an oxygen pumping shaft pipe through a sliding way; the oxygen pumping shaft pipe and the soaking cylinder are divided by the spiral blades to form independent cavities, so that fish schools with proper density are continuously propelled and uniformly soaked in compartments according to a set propelling speed; the oxygen dissolving level of the vaccine soaking solution is dynamically maintained through the oxygen pumping shaft tube; the fish school state is monitored in real time based on an image acquisition device, and abnormal individuals are marked; the vaccine soak solution is recycled through the separation grid plate and the recovery system. The problems that traditional artificial inoculation is large in batch difference, low in inoculation efficiency, unstable in dissolved oxygen and large in fish school stress are solved.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a continuous immersion inoculation method for fishery vaccines based on the Archimedean screw principle. Background Art

[0002] Traditional fishery vaccine immersion vaccination relies on manual operation, which has problems such as low efficiency, large differences between batches, difficulty in controlling dissolved oxygen in the immersion liquid, low utilization rate of the vaccine immersion liquid, and high stress on fish schools. Summary of the Invention

[0003] In order to overcome the defects of the existing technology, a continuous immersion inoculation method for fishery vaccines based on the Archimedes screw principle is provided to solve the problems of large differences between batches of traditional artificial inoculation, low inoculation efficiency, unstable dissolved oxygen, and greater stress on fish schools.

[0004] To achieve the above object, a method for continuous immersion inoculation of fishery vaccines based on the Archimedean screw principle is provided, comprising the following steps: The invention provides an immersion cylinder, a supporting plate, a separation grid, an image acquisition device and a controller, wherein the immersion cylinder is coaxially rotatably provided with an oxygenating shaft tube, the oxygenating shaft tube is connected to the oxygenating shaft tube, a plurality of oxygen discharge holes are opened on the oxygenating shaft tube, the oxygenating shaft tube is formed with a spiral blade arranged along an Archimedean spiral, the outer edge of the spiral blade is in contact with the inner wall of the immersion cylinder, a plurality of cavities are formed between the spiral blade and the inner wall of the immersion cylinder, the oxygenating shaft tube is transmission-connected to a driving mechanism, the supporting plate is formed with an inclined slideway, the lower end of the slideway extends into the inlet end of the immersion cylinder, a recovery box is installed below the outlet end of the immersion cylinder, the recovery box is connected to a preparation tank through a reflux pipe, the preparation tank is connected to the slideway through a distribution pipe, a delivery pump is installed on the distribution pipe, a separation grid is provided between the box opening of the recovery box and the outlet end, the image acquisition device is aligned with the interior of the immersion cylinder, and the controller is connected to the oxygenating equipment, the driving mechanism, the delivery pump and the image acquisition device; The fish school is put into the slideway, and at the same time, the controller starts the delivery pump to deliver the vaccine soaking liquid in the preparation tank to the slideway, so that the fish school and the vaccine soaking liquid are input into the inlet end of the soaking cylinder together; The controller activates the driving mechanism to rotate the oxygenating shaft tube, so that the fish school and the vaccine soaking liquid enter the multiple cavities and are discharged through the outlet end of the soaking cylinder. At the same time, the controller activates the oxygen enrichment device to pass oxygen into the vaccine soaking liquid in the cavity through the oxygen discharge hole of the oxygenating shaft tube, so that the dissolved oxygen concentration in the vaccine soaking liquid is within a preset range; The image acquisition device acquires images of the fish school in the immersion cylinder; The controller acquires the image to identify abnormal individuals in the school of fish; The vaccine immersion liquid and the fish school in the cavity are output to the separation grid through the outlet end to separate the vaccine immersion liquid from the fish school. The vaccine immersion liquid flows into the recovery box and is recycled through the reflux pipe, while the abnormal individuals in the fish school are removed.

[0005] Furthermore, the slideway has an inclination angle of 15° to 30°.

[0006] Furthermore, the delivery speed of the vaccine soaking solution is adapted to the delivery speed of the fish school.

[0007] Furthermore, the number of the cavities is 6 to 10, and the rotation speed of the oxygenating shaft tube is 1 to 10 rpm.

[0008] Furthermore, the oxygen pressure of the oxygenating shaft tube ranges from 0.1 to 0.3 MPa.

[0009] Furthermore, the rotation speed of the oxygenating shaft tube is 1-10 rpm.

[0010] The beneficial effect of the present invention is that the continuous immersion vaccination method for fishery vaccines based on the Archimedes screw principle of the present invention integrates the process flow of vaccine immersion, dissolved oxygen maintenance, and fish school monitoring, and synchronously transports the fish school and the vaccine immersion liquid through the slide of the support plate in turn. The buffering and lubrication of the vaccine immersion liquid can effectively avoid damage to the fish body. The spiral blades similar to the Archimedes screw in the immersion cylinder are used to divide the independent compartments to ensure that the fish school of appropriate density is evenly immersed and the stress of crowding is reduced. The dissolved oxygen level in the vaccine immersion liquid is adjusted in real time by oxygenating the oxygen shaft tube to ensure the activity of the fish school in each compartment and prevent hypoxia stress. The image acquisition device collects images of the fish school for the controller to identify abnormal fish individuals, thereby improving the safety of vaccination. After the fish school and the vaccine immersion liquid are separated by the separation grid, the vaccine immersion liquid is efficiently recycled after being formulated, thereby reducing the cost of vaccination. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Other features, objects and advantages of the present application will become more apparent upon reading the detailed description of non-limiting embodiments made with reference to the following drawings: Figure 1 This is a process flow chart of a continuous immersion inoculation method for fishery vaccines based on the Archimedean screw principle according to an embodiment of the present invention.

[0012] Figure 2 This is a schematic structural diagram of a continuous immersion vaccination device for fishery vaccines according to an embodiment of the present invention.

[0013] Figure 3 Schematic diagram of the structure of a support plate according to an embodiment of the present invention.

[0014] Figure 4 Schematic diagram of the structure of the separation grid plate according to an embodiment of the present invention.

[0015] Figure 5 Schematic diagram of the structure of the driving mechanism of an embodiment of the present invention.

[0016] Figure 6 Schematic diagram of the structure of an image acquisition device according to an embodiment of the present invention. DETAILED DESCRIPTION

[0017] The present application will be further described in detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are merely for the purpose of explaining the relevant invention and are not intended to limit the invention. It should also be noted that, for ease of description, only portions relevant to the invention are shown in the accompanying drawings.

[0018] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in this application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0019] The present invention provides a method for continuous immersion inoculation of fishery vaccines based on the Archimedes screw principle, which is implemented by continuous immersion inoculation equipment for fishery vaccines based on the Archimedes screw principle.

[0020] Specifically, refer to Figures 2 to 6 As shown, the continuous immersion vaccination equipment for fishery vaccines based on the Archimedean screw principle of the present invention includes: an immersion cylinder 1, an oxygenating shaft tube 2, a support plate 3, a preparation tank, an image acquisition device 5, and a controller.

[0021] In this embodiment, the soaking cylinder 1 is cylindrical and placed horizontally, and may be slightly tilted. The soaking cylinder 1 has an inlet end and an outlet end opposite to each other.

[0022] As a preferred embodiment, the wall of the soaking tube 1 is a transparent wall. In this embodiment, the wall of the soaking tube is transparent so that the internal situation of the soaking tube can be observed from the outside.

[0023] The oxygenating shaft tube 2 is coaxially and rotatably penetrated in the soaking cylinder 1. One end of the oxygenating shaft tube 2 is connected to an oxygenating device.

[0024] In this embodiment, the oxygen enrichment device is an oxygen enrichment cone.

[0025] The oxygenating shaft tube 2 is provided with multiple oxygen discharge holes. The diameter of the oxygen discharge holes is less than 2 mm, and the spacing between the oxygen discharge holes is 5 cm. The oxygenating shaft tube 2 is formed with spiral blades 21 arranged along an Archimedean spiral. The outer edges of the spiral blades are in contact with the inner wall of the immersion tube 1. Multiple cavities are formed between the spiral blades and the inner wall of the immersion tube 1. The oxygenating shaft tube 2 is connected to a drive mechanism 22.

[0026] In this embodiment, the oxygenating shaft and spiral blades form an Archimedean screw. The immersion cylinder is 2 meters long, and the oxygenating shaft is 0.5 meters long. The spiral blades divide the interior of the immersion cylinder into eight cavities. The lower portions of the eight cavities are independent, while the upper portions of the eight cavities are connected.

[0027] The oxygenating shaft is driven by a drive mechanism at a controlled speed of 1-10 rpm, thereby maintaining the dissolved oxygen concentration of the vaccine soaking solution in the soaking cylinder at 6-8 mg / L. The oxygen pressure of the oxygenating shaft is adjusted to 0.2 MPa.

[0028] The supporting plate 3 is formed with an inclined slide 30. The lower end of the slide 30 extends into the inlet end.

[0029] See Figure 3 As shown, the support plate 3 is tilted. In this embodiment, the tilt angle of the support plate is 15° to 30°. The width of the support plate gradually decreases from top to bottom. Baffles are formed on opposite sides of the support plate. A slideway is formed between the baffles and the support plate.

[0030] The preparation tank is used to hold the vaccine soaking solution. It is connected to the slideway 30 via a delivery pipe 43. A delivery pump 45 is mounted on the delivery pipe. A recovery tank 41 is mounted below the outlet of the soaking cylinder 1. This recovery tank 41 is connected to the preparation tank via a return pipe. A separation grid 42 is positioned between the opening of the recovery tank 41 and the outlet of the soaking cylinder 1.

[0031] Combine Figure 2 and Figure 4 As shown, the separation grid is curved. The inner curved surface of the separation grid faces upward. The separation grid is tilted. The upper end of the separation grid is mounted on the frame via a support plate. An opening is defined in the center of the separation grid. A grate is installed within the opening. The grate separates the fish from the vaccine solution, with the fish remaining on the grate while the vaccine solution flows through the grate into the opening of the recovery tank.

[0032] In this embodiment, a filter is installed in the box opening of the recovery box 41. The recovery rate of the vaccine soaking liquid is 98%, and the filtration accuracy is 5μm. After being prepared in the preparation tank, the concentration of the vaccine soaking liquid is maintained at ~10 6 CFU / ml.

[0033] The image acquisition device 5 is aligned with the interior of the soaking cylinder 1. In this embodiment, the image acquisition device is a camera.

[0034] The controller is connected to the oxygenation equipment, the driving mechanism, the delivery pump and the image acquisition device 5.

[0035] As a preferred embodiment, the continuous immersion vaccination device for fishery vaccines further comprises a mobile base 6. The immersion cylinder 1, the oxygenating shaft tube 2, the support plate 3, the recovery box 41 and the separation grid plate 42 are integrally mounted on the mobile base 6.

[0036] In this embodiment, a plurality of rollers are installed at the bottom of the mobile base. A frame is provided on the mobile base. The soaking cylinder is installed on the frame. Figure 2 and Figure 6 As shown, the image acquisition device 5 is a camera. The camera is arranged at the inlet end. The two ends of the oxygenating shaft tube are respectively mounted on the frame through bearings.

[0037] The oxygen pressure within the cavity is regulated by an oxygen-dissolving cone to ensure an appropriate dissolved oxygen concentration in the vaccine soaking solution. Oxygen is introduced into the oxygenating shaft, through oxygen discharge holes uniformly arranged on its surface, through which oxygen is released into the vaccine soaking solution within the cavity. The spiral blades are integrally connected to the oxygenating shaft and assembled via an Archimedean spiral keyway on the inner wall of the shaft. The shaft is driven by a drive mechanism for synchronous rotation.

[0038] As a preferred embodiment, the driving mechanism is a motor, and the output shaft of the motor is connected to the oxygenating shaft tube 2.

[0039] For details, see Figure 5 As shown, one end of the oxygen pumping shaft tube is coaxially connected to a driven gear. The output shaft of the motor of the driving mechanism is coaxially connected to a driving gear. The driving gear is meshed with the driven gear.

[0040] Combine Figure 1 As shown, the continuous immersion inoculation method for fishery vaccines based on the Archimedean screw principle of the present invention comprises the following steps: S1. Put the fish into the slide 30 of the support plate 3. At the same time, the controller starts the delivery pump to deliver the vaccine soaking liquid in the preparation tank to the slide 30, so that the fish and the vaccine soaking liquid are input into the inlet end of the soaking cylinder 1 together.

[0041] S2. The controller turns on the driving mechanism to rotate the oxygenating shaft tube 2, so that the fish and vaccine soaking liquid enter the multiple cavities and are output through the outlet end of the soaking cylinder 1. At the same time, the controller turns on the oxygen enrichment equipment to pass oxygen into the vaccine soaking liquid in the cavity through the oxygen discharge hole of the oxygenating shaft tube 2, so that the dissolved oxygen concentration in the vaccine soaking liquid is within a preset range.

[0042] S3. The image acquisition device 5 acquires images of the fish school in the immersion tube 1.

[0043] S4. The controller acquires images to identify abnormal individuals in the fish school.

[0044] The controller mainly identifies abnormal fish individuals based on convolutional neural networks. Abnormal signs include body surface damage, abnormal swimming behavior and body posture, etc.

[0045] S5. The vaccine immersion liquid and the fish school in the cavity are output to the separation grid plate 42 through the outlet end to separate the vaccine immersion liquid from the fish school. The vaccine immersion liquid flows into the recovery box 41 and is recycled through the reflux pipe, while the abnormal fish individuals are removed.

[0046] The fish and the vaccine soaking solution are synchronously transported through the slide of the support plate to the inlet of the soaking cylinder. Continuous soaking is carried out in the soaking cylinder. The spiral blades in the Archimedean screw are used to divide the compartments into multiple independent compartments. The rotation of the screw drives the fish to move continuously within the compartments and soak them evenly.

[0047] The oxygenation cone dynamically controls the dissolved oxygen through the oxygenating shaft tube, and continuously introduces oxygen into the vaccine soaking liquid through the oxygenating shaft tube to maintain the dissolved oxygen concentration within the preset range.

[0048] The status of fish schools is monitored through image acquisition devices, and the images of fish schools in the compartments are captured in real time through cameras, and abnormal fish individuals are identified and marked.

[0049] The fish and vaccine soaking liquid are separated by a separation grid, and the filtered vaccine soaking liquid is recovered by the recovery tank through the filter and sent to the preparation tank. After comprehensive treatment of the vaccine liquid in the preparation tank, it is recycled.

[0050] In this embodiment, the recovery rate of the vaccine soaking liquid is ≥98%, and the recovered liquid is filtered to remove solid impurities (particles ≥5 mm).

[0051] The continuous immersion vaccination method for fishery vaccines based on the Archimedes screw principle of the present invention synchronously transports fish and vaccine soaking liquid through the slide of the support plate, and the buffering lubrication of the vaccine soaking liquid can effectively avoid damage to the fish body. The spiral blades similar to the Archimedes screw in the soaking cylinder are used to divide the independent compartments to ensure that fish of appropriate density are evenly soaked and the stress of crowding is reduced. The dissolved oxygen level in the vaccine soaking liquid is adjusted in real time by oxygenating the oxygenating shaft tube to ensure the activity of the fish in each compartment and prevent hypoxia stress. The image acquisition device collects images of the fish school for the controller to identify abnormal fish individuals, thereby improving the safety of vaccination. After the fish school and the vaccine soaking liquid are separated by the separation grid, the vaccine soaking liquid is efficiently recycled after being formulated, thereby reducing the cost of vaccination.

[0052] The above description is merely a preferred embodiment of the present application and an illustration of the technical principles employed. Those skilled in the art should understand that the scope of the invention herein is not limited to the technical solutions formed by the specific combination of the above-mentioned technical features, but also encompasses other technical solutions formed by any combination of the above-mentioned technical features or their equivalents without departing from the inventive concept. For example, a technical solution formed by replacing the above-mentioned features with (but not limited to) technical features having similar functions disclosed in this application.

Claims

1. A method for continuous immersion inoculation of fishery vaccines based on the Archimedean screw principle, characterized in that: The following steps are involved: The invention provides an immersion cylinder, a supporting plate, a separation grid, an image acquisition device and a controller, wherein the immersion cylinder is coaxially rotatably provided with an oxygenating shaft tube, the oxygenating shaft tube is connected to the oxygenating shaft tube, a plurality of oxygen discharge holes are opened on the oxygenating shaft tube, the oxygenating shaft tube is formed with a spiral blade arranged along an Archimedean spiral, the outer edge of the spiral blade is in contact with the inner wall of the immersion cylinder, a plurality of cavities are formed between the spiral blade and the inner wall of the immersion cylinder, the oxygenating shaft tube is transmission-connected to a driving mechanism, the supporting plate is formed with an inclined slideway, the lower end of the slideway extends into the inlet end of the immersion cylinder, a recovery box is installed below the outlet end of the immersion cylinder, the recovery box is connected to a preparation tank through a reflux pipe, the preparation tank is connected to the slideway through a distribution pipe, a delivery pump is installed on the distribution pipe, a separation grid is provided between the box opening of the recovery box and the outlet end, the image acquisition device is aligned with the interior of the immersion cylinder, and the controller is connected to the oxygenating equipment, the driving mechanism, the delivery pump and the image acquisition device; The fish school is put into the slideway, and at the same time, the controller starts the delivery pump to deliver the vaccine soaking liquid in the preparation tank to the slideway, so that the fish school and the vaccine soaking liquid are input into the inlet end of the soaking cylinder together; The controller activates the driving mechanism to rotate the oxygenating shaft tube, so that the fish school and the vaccine soaking liquid enter the multiple cavities and are discharged through the outlet end of the soaking cylinder. At the same time, the controller activates the oxygen enrichment device to pass oxygen into the vaccine soaking liquid in the cavity through the oxygen discharge hole of the oxygenating shaft tube, so that the dissolved oxygen concentration in the vaccine soaking liquid is within a preset range; The image acquisition device acquires images of the fish school in the immersion cylinder; The controller acquires the image to identify abnormal individuals in the school of fish; The vaccine immersion liquid and the fish school in the cavity are output to the separation grid through the outlet end to separate the vaccine immersion liquid from the fish school. The vaccine immersion liquid flows into the recovery box and is recycled through the reflux pipe, while the abnormal individuals in the fish school are removed.

2. The continuous immersion inoculation method for fishery vaccines based on the Archimedes screw principle according to claim 1, characterized in that: The slideway has an inclination angle of 15° to 30°.

3. The continuous immersion inoculation method for fishery vaccines based on the Archimedes screw principle according to claim 1, characterized in that: The delivery speed of the vaccine soaking solution is adapted to the delivery speed of the fish school.

4. The continuous immersion inoculation method for fishery vaccines based on the Archimedes screw principle according to claim 3, characterized in that: The number of the cavities is 6 to 10, and the rotation speed of the oxygenating shaft tube is 1 to 10 rpm.

5. The continuous immersion inoculation method for fishery vaccines based on the Archimedes screw principle according to claim 1, characterized in that: The oxygen pressure of the oxygenating shaft tube ranges from 0.1 to 0.3 MPa.

6. The continuous immersion inoculation method for fishery vaccines based on the Archimedes screw principle according to claim 1, characterized in that: The rotation speed of the oxygenating shaft tube is 1-10 rpm.

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