Reagent bottle and immunohistochemical staining instrument

Abstract

The invention discloses a reagent bottle and an immunohistochemical stainer, the reagent bottle drives a ball screw to rotate through a motor, so that a ball nut and a rubber piston in a bottle body move, and a reagent is extruded into a pipette; a sealing ring is arranged at the liquid taking opening in the top of the bottle body and can realize sealing before and after the pipette tip is inserted. The immunohistochemical staining instrument is provided with a workbench, a machine head capable of moving in three directions is arranged above the workbench, and a plurality of pipettes are mounted at the bottom of the machine head; the table top of the workbench is configured into an operation area, and a reagent storage area and a glass slide storage area which are positioned on two sides of the operation area, and the reagent storage area and the glass slide storage area are respectively provided with a reagent box and a glass slide box which are arranged in a layered manner; the reagent platform in the kit and the glass slide platform in the glass slide box can be pulled out or withdrawn towards the operation area. The problems that a traditional immunohistochemical staining instrument is low in pipetting and staining speed, inaccurate in liquid taking amount, large in occupied space, capable of volatilizing reagents and the like are solved.

Description

Technical Field

[0001] The invention relates to medical equipment, in particular to a reagent bottle and an immunohistochemical staining instrument. Background Art

[0002] Immunohistochemical staining is a technique that utilizes the principle of specific binding of antigen and antibody, and makes the developer (such as fluorescein, enzyme, metal ion, etc.) of labeled antibody develop color by chemical reaction, so as to determine the position and content change of antigen (such as polypeptide, protein) in tissue cells. Immunohistochemical staining needs to remove the reagent in the reagent bottle by a pipette, and then perform staining operation on a slide. On the one hand, the existing reagent bottle is an open bottle with a stopper, and the reagent is drawn by a pipette, and there is a problem that the amount of liquid transferred is not accurate enough, and the reagent bottle opening easily causes the reagent to volatilize, threatening the health of researchers. On the other hand, the existing immunohistochemical staining instrument mostly adopts a single pipette to carry out pipetting, and the reagent and slide are arranged in a single layer. Since only one reagent can be taken at a time, the pipette tip needs to be replaced in time, which is cumbersome to operate, and the efficiency of pipetting and staining is low. Summary of the Invention

[0003] Purpose of the invention: The first purpose of the present invention is to provide a reagent bottle that can improve the accuracy of pipetting and prevent the volatilization of reagents; the second purpose of the present invention is to provide an immunohistochemical staining instrument with high pipetting and staining efficiency.

[0004] Technical solution: A reagent bottle of the present invention includes a bottle body, a rubber piston is slidably arranged in the bottle body, the reagent is stored in the upper cavity of the rubber piston, and a scale reflecting the amount of reagent is provided on the bottle body; a liquid taking port for inserting a pipette tip is provided on the top of the bottle body, and a sealing ring is provided at the liquid taking port, which can achieve sealing before and after the insertion of the pipette tip; a ball nut is fixed to the lower side of the rubber piston through a piston bracket, the ball screw cooperates with the ball nut, and the ball screw passes downward out of the bottle body; when the ball screw is driven to rotate by a motor, it can drive the ball nut and the rubber piston to move upward, so that the reagent is squeezed into the pipette.

[0005] An immunohistochemical staining instrument of the present invention comprises a workbench and a machine head, wherein the machine head is arranged above the workbench and can move along the X, Y and Z axes; the workbench table is configured as an operation area and a reagent storage area and a slide storage area located on both sides of the operation area; the reagent storage area has a reagent kit arranged in layers, and the slide storage area has a slide box arranged in layers; the reagent platform in the reagent kit can be drawn out or retracted toward the operation area, and the slide platform in the slide box can be drawn out or retracted toward the operation area; the slide platform is provided with a plurality of slides, and the reagent platform is provided with a plurality of reagent bottles fixed side by side; the reagent platform has a cavity inside, in which a micro motor is provided, and the micro motor simultaneously drives ball screws at the bottom of a plurality of reagent bottles through a transmission mechanism; and a plurality of pipettes are installed at the bottom of the machine head.

[0006] Furthermore, both the reagent platform and the slide platform are drawer-type structures.

[0007] Furthermore, the reagent kits arranged in layers are grouped together, and the reagent kits are configured into multiple groups, with each group arranged side by side.

[0008] Furthermore, the slide boxes arranged in layers are grouped as one, and the slide boxes are configured into multiple groups, each group being arranged side by side; the number of groups of slide boxes is equal to the number of groups of the reagent kit.

[0009] Furthermore, each test kit is provided with a temperature regulating device, and each temperature regulating device controls the temperature independently.

[0010] Furthermore, the immunohistochemical staining instrument also includes a Y-direction moving device, an X-direction moving device and a Z-direction moving device, the Z-direction moving device is used to drive the head to move along the Z direction, the X-direction moving device is used to drive the Z-direction moving device and the head to move along the X direction, and the Y-direction moving device is used to drive the X-direction moving device, the Z-direction moving device and the head to move along the Y direction.

[0011] Furthermore, the Y-axis moving device includes a first motor, a first transmission shaft and two first bearing seats arranged parallel to each other on both sides of the workbench, the first bearing seat is provided with a first screw guide rail and a first nut slider, and the end of the first bearing seat is provided with a first worm gear; the first motor transmits power to the two first worm gears through the transmission shaft, thereby driving the two first nut sliders to move synchronously along the corresponding first screw guide rails; the X-axis moving device is installed on the two first nut sliders.

[0012] Furthermore, the X-axis moving device includes an equal-strength beam, a second motor and two columns, the two columns are respectively fixed on the two first nut sliders, the two columns are equipped with second bearing seats, the second bearing seats are equipped with second screw guide rails and second nut sliders, the equal-strength beam is fixed to the sides of the two columns and supports the second screw guide rails; the second motor is used to drive the second nut slider to move along the second screw guide rails and the equal-strength beam; the Z-axis moving device is installed on the second nut slider.

[0013] Furthermore, the Z-direction moving device includes a third motor and a third bearing seat, on which a third screw guide rail and a third nut slider are mounted, and the third nut slider is fixed on the second nut slider; the third motor drives the third screw guide rail to move along the Z direction, and the machine head is mounted at the bottom of the third screw guide rail.

[0014] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages:

[0015] (1) The present invention drives the ball screw through a motor to move the ball nut and the rubber piston, squeezing the reagent into the pipette gun. The pipetting amount corresponds to the displacement of the ball nut, thereby ensuring the accuracy of the pipetting amount.

[0016] (2) Rubber piston and sealing ring prevent reagent leakage.

[0017] (3) The present invention can move multiple reagents or stain multiple slides at the same time, thereby improving the efficiency of pipetting and staining.

[0018] (4) The immunohistochemical staining instrument has a compact structure and takes up little space. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 Schematic diagram of the structure of the immunohistochemical staining instrument provided by an embodiment of the present invention;

[0020] Figure 2 Schematic diagram of the structure of the workbench and the Y-axis moving device in an embodiment of the present invention;

[0021] Figure 3 2 is a schematic structural diagram of an X-direction moving device according to an embodiment of the present invention;

[0022] Figure 4 2 is a schematic structural diagram of a Z-direction moving device according to an embodiment of the present invention;

[0023] Figure 5 Schematic diagram of the reagent storage area and the slide storage area in an embodiment of the present invention;

[0024] Figure 6 This is a schematic structural diagram of a reagent bottle in an embodiment of the present invention;

[0025] Figure 7Schematic diagram of the structure of the micro motor and transmission mechanism inside the reagent platform in an embodiment of the present invention. DETAILED DESCRIPTION

[0026] The present invention will be further described below with reference to the accompanying drawings.

[0027] Attachment Figures 1 to 6 The reference numerals in the figures are as follows:

[0028] 1. Workbench;

[0029] 2, Y-axis moving device; 21, first bearing seat; 22, first screw guide rail; 23, first nut slider; 24, first motor; 25, first worm gear; 26, first transmission shaft; 27, shaft protection housing;

[0030] 3, X-axis moving device; 31, column; 32, constant strength beam; 33, second screw guide rail; 34, second nut slider; 35, second motor; 36, second bearing seat;

[0031] 4, Z-axis moving device; 41, third motor; 42, third screw guide; 43, third bearing seat; 44, machine head; 441, pipette tip;

[0032] 5. Reagent storage area; 51. Reagent kit; 52. Reagent bottle; 521. Ball screw; 522. Ball nut; 523. Rubber piston; 524. Sealing ring; 525. Bottle body; 526. Micro motor; 527. Second worm gear; 528. Second transmission shaft; 53. Reagent platform;

[0033] 6, slide storage area; 61, slide box; 62, slide; 63, slide platform.

[0034] Example 1

[0035] like Figure 6 As shown, Example 1 provides a reagent bottle, including a bottle body 525, in which a rubber piston 523 is slidably disposed. The reagent is stored in a cavity above the rubber piston 523. A scale reflecting the reagent volume is provided on the bottle body 525. A liquid extraction port for inserting a pipette tip 441 is provided at the top of the bottle body 525. A sealing ring 524 is provided at the liquid extraction port. The sealing ring 524 can achieve a seal both before and after the pipette tip 441 is inserted.

[0036] A ball nut 522 is mounted on the underside of the rubber piston 523. A piston bracket is welded to the ball nut 522, tightly bonded to the rubber piston 523. A ball screw 521 engages with the ball nut 522 and extends downward through the bottle 525. When the motor rotates the ball screw 521, it drives the ball nut 522 and rubber piston 523 upward, squeezing the reagent into the pipette.

[0037] Example 2

[0038] like Figures 1 to 5 As shown, Example 2 provides an immunohistochemical staining instrument, including a workbench 1, a Y-axis moving device 2, an X-axis moving device 3 and a Z-axis moving device 4. The surface of the workbench 1 is configured as an operation area and a reagent storage area 5 and a slide storage area 6 located on both sides of the operation area.

[0039] The reagent storage area 5 has reagent kits 51 arranged in layers, and the slide storage area 6 has slide boxes 61 arranged in layers. The reagent kits 51 arranged in layers are grouped into three groups, each arranged side by side. The slide boxes 61 arranged in layers are grouped into three groups, each arranged side by side.

[0040] The reagent platform 53 in the reagent box 51 is a drawer-type structure, which can be drawn out or retracted into the operating area. The slide platform 63 in the slide box 61 is also a drawer-type structure, which can be drawn out or retracted into the operating area.

[0041] In this embodiment, a glass slide platform 63 is provided with a plurality of glass slides 62, and a reagent platform 53 is provided with two reagent bottles 52 fixed side by side. The reagent bottles 52 are the reagent bottles described in Example 1. In this embodiment, two grooves are provided side by side on the reagent platform 53, each groove having an internal thread, and the lower portion of the reagent bottle 52 has an external thread, and the reagent bottle 52 is threadedly connected to the groove.

[0042] Combine Figure 7 The reagent platform 53 has a cavity inside, in which a micromotor 526 is disposed. The micromotor 526 simultaneously drives the ball screws 521 at the bottom of the two reagent bottles 52 through a transmission mechanism. The transmission mechanism includes a second worm gear 527 and a second transmission shaft 528. The micromotor 526 transmits power to the two second worm gears 527 via the second transmission shaft 528, thereby driving the two ball screws 521 to rotate. Specifically, the two turbines are fixed to the second transmission shaft 528, which is connected to the output end of the micromotor 526, and the two worm gears are respectively connected to the two ball screws 521.

[0043] Different reagents have different storage temperatures, and long-term storage at room temperature can easily cause the reagents to deteriorate. For this reason, the present embodiment is provided with a temperature regulating device on each reagent box 51, and each temperature regulating device independently controls the temperature to meet the storage temperature requirements of different types of reagents and avoid reagent deterioration.

[0044] The Y-moving device 2 includes a first motor 24, a first transmission shaft 26 and two first bearing seats 21 arranged in parallel on both sides of the workbench 1. The first bearing seat 21 is provided with a first screw guide rail 22 and a first nut slider 23, and the end of the first bearing seat 21 is provided with a first worm gear 25. The first motor 24 transmits power to the two first worm gears 25 through the first transmission shaft 26, thereby driving the two first nut sliders 23 to move synchronously along the corresponding first screw guide rails 22. Specifically, the two turbines are fixed on the first transmission shaft 26, the first transmission shaft 26 is connected to the output end of the first motor 24, and the two worms are respectively connected to the two first screw guide rails 22. The first transmission shaft 26 is protected by a shaft protection shell 27.

[0045] The X-axis moving device 3 includes a constant-strength beam 32, a second motor 35, and two columns 31. The two columns 31 are respectively fixed to the two first nut sliders 23. Second bearing blocks 36 are mounted on the two columns 31, and the second bearing blocks 36 are provided with second screw guide rails 33 and second nut sliders 34. The constant-strength beam 32 is fixed to the sides of the two columns 31 and supports the second screw guide rails 33. The second motor 35 is used to drive the second nut sliders 34 to move along the second screw guide rails 33 and the constant-strength beam 32. The constant-strength beam 32 ensures that the middle section of the second screw guide rail 33 does not bend under gravity, thereby ensuring the accuracy of the X-axis displacement pipetting action.

[0046] The Z-direction movement device 4 includes a third motor 41 and a third bearing block 43. A third screw guide rail 42 and a third nut slider are mounted on the third bearing block 43. The third nut slider is fixed to the second nut slider 34. The third motor 41 drives the third screw guide rail 42 to move in the Z direction. A machine head 44 is mounted at the bottom of the third screw guide rail 42. Several pipette guns are mounted at the bottom of the machine head 44.

[0047] The Y-axis moving device 2, the X-axis moving device 3 and the Z-axis moving device 4 introduced in the embodiment of the present invention are mainly a linear slide module, which is a prior art.

[0048] During the dyeing process, when the machine head 44 needs to move in the Y direction, the first motor 24 is started, driving the two first nut sliders 23 to move in the Y direction, and then the X-direction moving device 3, the Z-direction moving device 4 and the machine head 44 move in the Y direction as a whole. When the machine head 44 needs to move in the X direction, the second motor 35 directly drives the second screw guide rail 33 to rotate, driving the second nut slider 34 to move in the X direction, and then the Z-direction moving device 4 and the machine head 44 move in the X direction as a whole. The third motor 41 drives the third screw guide rail 42. Since the third nut slider is fixed, the third screw guide rail 42 and the machine head 44 move in the Z direction. Thus, the three motors can jointly control the three-dimensional displacement of the machine head 44, move the reagent to the top of the specified slide 62, and release the reagent for dyeing. Since multiple pipette guns are installed at the bottom of the machine head 44, multiple reagents can be moved or multiple slides can be dyed at the same time, thereby improving the efficiency of pipetting and dyeing.

[0049] In summary, the present invention solves a series of problems existing in traditional immunohistochemical staining instruments, such as slow pipetting and staining speed, inaccurate liquid volume, large space occupation, and volatilization of staining agents.

Claims

1. A reagent bottle, comprising a bottle body (525), characterized in that: A rubber piston (523) is slidably provided in the bottle body (525), and the reagent is stored in the upper cavity of the rubber piston (523). A scale reflecting the amount of the reagent is provided on the bottle body (525); a liquid taking port for inserting the pipette tip (441) is provided on the top of the bottle body (525), and a sealing ring (524) is provided at the liquid taking port. The sealing ring (524) can achieve sealing before and after the pipette tip (441) is inserted; a ball nut (522) is fixed to the lower side of the rubber piston (523) through a piston bracket, and the ball screw (521) cooperates with the ball nut (522), and the ball screw (521) passes through the bottle body (525) downward; when the ball screw (521) is driven to rotate by the motor, it can drive the ball nut (522) and the rubber piston (523) to move upward, so that the reagent is squeezed into the pipette.

2. An immunohistochemical staining instrument, characterized in that: The invention comprises a workbench (1) and a machine head (44), wherein the machine head (44) is arranged above the workbench (1) and can move along the X, Y and Z axes; the workbench (1) table is configured as an operation area and a reagent storage area (5) and a slide storage area (6) located on both sides of the operation area; the reagent storage area (5) has a reagent box (51) arranged in layers; the slide storage area (6) has a slide box (61) arranged in layers; the reagent platform (53) in the reagent box (51) can be drawn out or drawn out toward the operation area; The slide platform (63) in the slide box (61) can be pulled out or retracted toward the operating area; the slide platform (63) has a plurality of slides (62), and the reagent platform (53) has a plurality of reagent bottles according to claim 1 fixed side by side; the reagent platform (53) has a cavity inside, and a micro motor (526) is provided in the cavity, and the micro motor (526) drives the ball screw (521) at the bottom of multiple reagent bottles at the same time through a transmission mechanism; and a plurality of pipette guns are installed at the bottom of the machine head (44).

3. The immunohistochemical staining instrument according to claim 2, characterized in that: The reagent platform (53) and the slide platform (63) are both drawer-type structures.

4. The immunohistochemical staining instrument according to claim 2, characterized in that: The reagent kits (51) arranged in layers are grouped together, and the reagent kits (51) are configured into multiple groups, each group being arranged side by side.

5. The immunohistochemical staining instrument according to claim 4, characterized in that: The slide boxes (61) arranged in layers are grouped together. The slide boxes (61) are configured into multiple groups, each group being arranged side by side. The number of groups of the slide boxes (61) is equal to the number of groups of the reagent kit (51).

6. The immunohistochemical staining instrument according to claim 2, characterized in that: Each reagent box (51) is provided with a temperature regulating device, and each temperature regulating device controls the temperature independently.

7. The immunohistochemical staining instrument according to any one of claims 2 to 6, characterized in that: The machine tool further comprises a Y-direction moving device (2), an X-direction moving device (3) and a Z-direction moving device (4). The Z-direction moving device (4) is used to drive the machine head (44) to move along the Z-direction, the X-direction moving device (3) is used to drive the Z-direction moving device (4) and the machine head (44) to move along the X-direction, and the Y-direction moving device (2) is used to drive the X-direction moving device (3), the Z-direction moving device (4) and the machine head (44) to move along the Y-direction.

8. The immunohistochemical staining instrument according to claim 7, characterized in that: The Y-direction moving device (2) comprises a first motor (24), a first transmission shaft (26) and two first bearing seats (21) arranged in parallel on both sides of the workbench (1); a first screw guide rail (22) and a first nut slider (23) are arranged on the first bearing seat (21); a first worm gear (25) is arranged at the end of the first bearing seat (21); the first motor (24) transmits power to the two first worm gears (25) through the transmission shaft (26), thereby driving the two first nut sliders (23) to move synchronously along the corresponding first screw guide rails (22); and the X-direction moving device (3) is mounted on the two first nut sliders (23).

9. The immunohistochemical staining instrument according to claim 8, characterized in that: The X-direction moving device (3) comprises an equal strength beam (32), a second motor (35) and two columns (31), the two columns (31) are respectively fixed on the two first nut sliders (23), the two columns (31) are mounted with second bearing seats (36), the second bearing seats (36) are provided with a second screw guide rail (33) and a second nut slider (34), the equal strength beam (32) is fixed to the sides of the two columns (31) and supports the second screw guide rail (33); the second motor (35) is used to drive the second nut slider (34) to move along the second screw guide rail (33) and the equal strength beam (32); and the Z-direction moving device (4) is mounted on the second nut slider (34).

10. The immunohistochemical staining instrument according to claim 9, characterized in that: The Z-direction moving device (4) comprises a third motor (41) and a third bearing seat (43); a third screw guide rail (42) and a third nut slider are mounted on the third bearing seat (43); the third nut slider is fixed on the second nut slider (34); the third motor (41) drives the third screw guide rail (42) to move along the Z direction; the machine head (44) is mounted on the bottom of the third screw guide rail (42).

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