A high-affinity glutamine-regulating intestinal flora protein powder and its preparation method

Abstract

This invention belongs to the field of functional food technology, specifically relating to a high-affinity glutamine intestinal flora regulating protein powder and its preparation method. The high-affinity glutamine intestinal flora regulating protein powder comprises 62-68% enzymatically hydrolyzed whey peptides, 19-21% acetylated glutamine, and 11-14% complex prebiotics. The preparation method includes: enzymatic hydrolysis, modification, post-biotic treatment, compounding, and drying. Through molecular-level synergistic design, whey peptides are enzymatically hydrolyzed at 600±50 Da to enrich ≥6% γ-glutamylglutamine dipeptide; targeted modification utilizes acetylated glutamine with an acetylation degree ≥98%, forming heteropeptide bonds; post-biotics are broken down by ice bath ultrasound to achieve a β-glucan exposure rate ≥82%, combining with glycopeptide complexes to achieve a glutamine bioavailability ≥95%, a bifidobacteria proliferation of more than 3 times, and a 40% increase in blood lactate clearance rate, while also possessing advantages such as solubility stability, fermentation promotion, and effervescent formulation.

Description

Technical Field

[0001] This invention belongs to the field of functional food technology, specifically relating to a high-affinity glutamine intestinal flora protein powder and its preparation method. Background Technology

[0002] Gut health and exercise function recovery are core research directions in modern nutrition. Glutamine, as the main energy source for intestinal epithelial cells, plays a crucial role in maintaining the integrity of the intestinal barrier, regulating the balance of gut microbiota, and promoting tissue repair after exercise. However, traditional glutamine supplements face significant technical bottlenecks: First, their oral bioavailability is low, with the acidic environment of the stomach causing over 40% degradation of the component, and free glutamine is easily competitively consumed by pathogenic bacteria in the intestine; second, a single component cannot simultaneously achieve gut microbiota regulation and mucosal repair, resulting in insufficient intestinal colonization efficiency and significant limitations in efficacy; third, existing formulations such as ordinary powders and tablets suffer from poor stability and slow absorption rates, failing to meet the demands of sports nutrition for rapid onset of action and portability.

[0003] In existing technologies, some solutions combine high-molecular-weight whey protein peptides (molecular weight > 2kDa) with free glutamine. However, high-molecular-weight peptides require secondary enzymatic hydrolysis in the intestine for absorption, resulting in low nutrient delivery efficiency. Furthermore, the combined system lacks a targeted protection mechanism, and glutamine exhibits high degradation rates in the stomach. Other studies have attempted to introduce prebiotics such as fructooligosaccharides to regulate gut microbiota, but the acid stability of the core components remains unresolved, and the synergistic mechanism is unclear. Regarding the application of postbiotics, traditional processes often involve the direct addition of inactivated bacterial cells, resulting in insufficient exposure of cell wall active components such as β-glucan, limiting immunomodulatory activity and intestinal adhesion. In addition, commercially available sports nutrition preparations generally suffer from poor solubility, reconstitution times exceeding 5 minutes, and a single dosage form limited to conventional powders, failing to balance storage and transportation stability with the end-user experience.

[0004] From a mechanistic perspective, ordinary whey protein peptides, due to their large molecular weight, only physically mix with glutamine, failing to form a stable protective structure. This results in both peptides being released separately in the gastrointestinal tract, increasing the risk of glutamine being destroyed by gastric acid and hindering the synergistic effect in improving intestinal absorption efficiency. While the addition of prebiotics alone can promote the proliferation of beneficial bacteria, the lack of a coordinated design with the glutamine delivery system fails to address the spatiotemporal synergy between gut microbiota regulation and mucosal repair. Limitations in the preparation process of postbiotics lead to insufficient release of their active ingredients, making it difficult to effectively activate the intestinal immune response and restricting their potential application in enhancing barrier function.

[0005] In terms of formulation technology, traditional protein powders, due to their high proportion of hydrophobic amino acids, are prone to forming intermolecular hydrogen bonds during storage, leading to clumping. Reconstitution requires prolonged stirring, affecting ease of use. Conventional dosage forms such as tablets have slow disintegration rates, failing to meet the need for rapid nutritional replenishment after exercise. Meanwhile, simple granule designs do not consider compatibility with the intestinal pH environment, potentially causing premature release or degradation of active ingredients in the stomach.

[0006] In summary, current technologies have not yet solved core problems such as low glutamine bioavailability, insufficient synergy between gut microbiota regulation and barrier repair, and suboptimal formulation performance. The root cause lies in the lack of multi-level technological innovation, encompassing molecular structure design, delivery system optimization, and synergistic effects of functional components. How to achieve targeted protection of glutamine, synergistic regulation of the gut microbiota-mucosal axis, and rapid absorption of nutrients through precise chemical modification, efficient active ingredient release mechanisms, and intelligent dosage form design has become a key technological challenge urgently needing breakthroughs in this field. Summary of the Invention

[0007] The purpose of this invention is to provide a high-affinity glutamine-regulating intestinal flora protein powder and its preparation method.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A high-affinity glutamine gut microbiota regulating protein powder, comprising, by weight percentage:

[0010] 62-68% enzymatically hydrolyzed whey protein peptides: 90wt% of peptides have a molecular weight of <1kDa, with an average molecular weight of 600±50Da, of which γ-glutamylglutamine dipeptide accounts for ≥6%, and hydrophobic amino acids account for ≤12%.

[0011] 19-21% acetylated glutamine: degree of acetylation ≥98%, free glutamine residue ≤0.5%;

[0012] 10-14% Complex Prebiotics: Fructooligosaccharide purity ≥95%, resistant dextrin solubility ≥85%, the mass ratio of fructooligosaccharide, resistant dextrin and galactooligosaccharide is 1:1.2:0.5, and it also contains 0.3%-0.5% polydextrose;

[0013] 3-5% inactivated Lactobacillus plantarum postbiotic powder: inactivated at 121℃ for 15 min and ultrasonically broken down in an 800W ice bath for 10 min, with a particle size D90≤1.5μm, extracellular polysaccharide ≥150mg / g, β-glucan exposure rate ≥80%, and short-chain fatty acid retention rate ≥90%.

[0014] Furthermore, the proportion of peptides containing 4-7 consecutive hydrophilic amino acids in the enzymatically hydrolyzed whey protein peptides is ≥30%, and the proportion of the complex of the enzymatically hydrolyzed whey protein peptides and acetylated glutamine is ≥88%.

[0015] Furthermore, the oligogalactose and polydextrose in the compound prebiotic undergo a Maillard reaction for 4 hours at 60°C and 75% relative humidity in the presence of whey protein peptides to generate a glycopeptide complex with a molecular weight of 500-2000 Da.

[0016] A method for producing a high-affinity glutamine-modifying intestinal flora protein powder includes:

[0017] (a) Enzymatic hydrolysis: The whey protein was hydrolyzed for 50 minutes at pH 7.0±0.2 and 58℃ using a complex protease containing 2.5% whey protein, with a mass ratio of neutral protease to trypsin of 4:1. The target peptide was then separated by a 10kDa ultrafiltration membrane.

[0018] (b) Modification: The enzymatically hydrolyzed peptide and acetylated glutamine were mixed at a mass ratio of 3.2:1, and 0.25% glutamine transaminase was added. The mixture was reacted at 42°C and pH 6.9 for 2.5 hours to form ε-(γ-glutamyl)lysine isopeptide bonds.

[0019] (c) Post-genetic treatment: After being inactivated at 121℃ for 15 min, Lactobacillus plantarum cells were ultrasonically disrupted at 800W for 10 min in an ice bath at ≤10℃ and then freeze-dried to obtain powder.

[0020] (d) Compounding: Mix the product of step (b), the compound prebiotic and postbiotic powder in a nitrogen atmosphere with an oxygen content of ≤0.1% for 15 minutes;

[0021] (e) Drying: Spray drying, with an inlet air temperature of 58°C and an outlet air temperature of 33°C, controlling the powder moisture content to be ≤3.2% and the particle size D50 to be 18±2μm.

[0022] An effervescent granule containing a high-affinity glutamine gut microbiota regulating protein powder, comprising: 70-75% protein powder, an effervescent system composed of citric acid and sodium bicarbonate with a mass ratio of citric acid to sodium bicarbonate of 1:1.3, and a 3% hydroxypropyl methylcellulose coating layer.

[0023] Performance: Particle size D50 = 500-800μm, foaming time upon contact with water ≤25 seconds, resolvable pH 6.0-6.5, water vapor transmission rate ≤0.1g / (m³) 2 ·day);

[0024] Process: Dry granulation, tableting pressure 10-15kN, single tablet weight 5g, disintegration time ≤1 minute.

[0025] Application of a high-affinity glutamine gut microbiota regulating protein powder in the preparation of post-exercise recovery formulations:

[0026] The compound formulation consists of protein powder, coconut water powder, and electrolytes in a mass ratio of 85:10:5; the electrolyte is a mixture of sodium chloride and potassium chloride, wherein the mass ratio of sodium chloride to potassium chloride is 3:1.

[0027] Physical properties: Reconstitution time at 40℃ ≤ 2 minutes, osmotic pressure 270±20 mOsm / kg;

[0028] Dosage instructions: The single dose of the preparation is 20g, which contains 17g of protein powder. Take it within 30 minutes after exercise.

[0029] Application of a high-affinity glutamine gut microbiota regulating protein powder in the preparation of functional foods that improve leaky gut syndrome:

[0030] Dosage: Take 25g daily, divided into two doses, for 21 consecutive days;

[0031] Biomarker detection: Patients' serum DAO enzyme activity decreased by ≥45%, fecal calprotectin ≤50μg / g, and tight junction protein ZO-1 expression increased by 2-fold;

[0032] Structural characteristics: The β-glucan exposure rate of the postgenetic powder is ≥80%.

[0033] Application of a high-affinity glutamine gut microbiota regulating protein powder as a yogurt fermentation promoter:

[0034] Addition amount: The addition amount is 4% of the milk base mass, of which the post-biotic powder accounts for 4% of the protein powder mass;

[0035] Fermentation conditions: Ferment at 42℃ for ≤6 hours, yielding yogurt with a live bacteria count ≥1×10⁻⁶. 10 CFU / mL, water holding capacity ≥85%;

[0036] Product characteristics: The finished yogurt has a pH of 4.2-4.5 and an acetaldehyde content of ≥25mg / kg.

[0037] A detection method for high-affinity glutamine gut microbiota-regulating protein powder:

[0038] In vitro microbial culture: Human fecal microbiota were inoculated and cultured under anaerobic conditions at 37°C for 24 hours. The changes in the number of Bifidobacteria were determined by plate counting.

[0039] Simulated digestion: The gastrointestinal digestion model was performed using the USP dissolution method, and the glutamine release was determined by HPLC.

[0040] The beneficial effects of this invention are as follows:

[0041] 1. Significantly improves glutamine bioavailability. Acetylated glutamine forms covalent isopeptide bonds through transaminase catalysis, and combined with the carrier effect of small molecule peptides, the bioavailability of glutamine can reach over 95% in a simulated gastrointestinal digestive environment. In contrast, the bioavailability of free glutamine is less than 50%, and this technology effectively avoids the degradation of glutamine by gastric acid.

[0042] 2. Enhanced gut microbiota colonization and barrier repair. After ultrasonic disruption, the β-glucan exposure rate of the prebiotics reached over 82%. Synergistically, this prebiotic, combined with a glycopeptide complex (fructooligosaccharides, resistant dextrin, and galactooligosaccharides in a 1:1.2:0.5 ratio), resulted in a more than 3-fold increase in Bifidobacterium proliferation in human fecal microbiota culture experiments. Clinical data showed that patients experienced a more than 45% decrease in serum DAO enzyme activity and a 2.1-fold increase in ZO-1 protein expression, indicating a significant effect on gut barrier repair.

[0043] 3. Enables rapid post-exercise recovery. The formulation contains compound electrolytes (NaCl to KCl ratio of 3:1) and coconut water powder, with an osmotic pressure controlled at 270±20 mOsm / kg, matching the isotonic absorption characteristics of the intestine. Experiments show that after a single dose of 20g of the formulation, the blood lactate clearance rate increases by 40%, which can meet the nutritional needs of the golden 30-minute window after exercise and accelerate post-exercise functional recovery.

[0044] 4. Overcoming the bottleneck of protein powder solubility stability. The hydrophobic amino acid content of the enzymatically hydrolyzed peptides is less than 12%, and it contains more than 30% continuous hydrophilic peptide segments, ensuring that the powder resolvates in water at 40℃ for no more than 2 minutes. After spray drying, the powder angle of repose is ≤30°, and the flowability is improved by 35% compared with similar products, effectively solving the clumping problem and significantly improving the product's solubility stability.

[0045] 5. Expanding the application scenarios of highly active fermented milk. As a yogurt fermentation promoter, when added at a concentration of 4%, the content of metagenic extracellular polysaccharides is ≥150mg / g, which can stimulate the proliferation of lactic acid bacteria, shortening the fermentation time to less than 6 hours (compared to 8 hours in the control group), and the viable count of the finished product is ≥1×10⁻⁶. 10 With CFU / mL and acetaldehyde flavor substance content ≥25mg / kg, it broadens the application scenarios of highly active fermented milk.

[0046] 6. Innovative dosage form ensures a superior end-user experience. Effervescent granules are coated with hydroxypropyl methylcellulose, with a water vapor permeability ≤0.12g / (m³). 2 The product disintegrates in cold water for ≤1 minute, with a pH value controlled between 6.0 and 6.5, making it suitable for the slightly alkaline environment of the intestines. The aluminum-plastic packaging design extends the product's shelf life to 24 months, effectively ensuring a positive end-user experience. Attached Figure Description

[0047] Figure 1A bar chart showing the fold increase of Bifidobacteria.

[0048] Figure 2 A bar chart showing the rate of decrease in serum DAO enzyme activity. Detailed Implementation

[0049] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0050] Example 1: Protein powder preparation and performance testing

[0051] Raw materials and processes:

[0052] 1. Enzymatic hydrolysis:

[0053] Take 1000g of whey protein and add 25g of complex protease (20g of neutral protease and 5g of trypsin);

[0054] Hydrolyze at pH 7.0 and 58℃ for 50 minutes, inactivate enzymes in boiling water bath, and separate using a 10kDa ultrafiltration membrane to obtain peptides with a molecular weight <1kDa (HPLC analysis: average molecular weight 602Da, γ-glutamylglutamine dipeptide 6.8%, hydrophobic amino acid 10.2%).

[0055] 2. Modification:

[0056] Mix 680g of enzymatically hydrolyzed peptides with 210g of acetylated glutamine (98.5% degree of acetylation, 0.3% free glutamine), and add 2.2g of transglutaminase.

[0057] The reaction was carried out at 42℃ and pH 6.9 for 2.5 hours to form a complex (HPLC analysis showed that the complex accounted for 89.1%, and 32% of it contained ≥5 consecutive hydrophilic amino acid peptides).

[0058] 3. Postgenerative treatment:

[0059] Lactobacillus plantarum cells were inactivated at 121℃ for 15 min and then ultrasonically disrupted at 800W for 10 min in an ice bath (particle size D90 = 1.3 μm).

[0060] Freeze-drying yielded a powder (extracellular polysaccharide 158 mg / g, β-glucan exposure rate 82%, short-chain fatty acid retention rate 92%).

[0061] 4. Compounding and drying:

[0062] Mix 890g of the modified product, 120g of the compound prebiotic (fructooligosaccharide: resistant dextrin: galactooligosaccharide = 40g: 48g: 20g, polydextrose 4g), and 40g of post-biogener powder under nitrogen with an oxygen content of 0.08% for 15min.

[0063] Spray drying (inlet air 58℃ / outlet air 33℃) yields powder (moisture content 2.9%, D50 = 17μm).

[0064] Performance verification:

[0065] Maillard reaction product: The reaction of compound prebiotics and whey protein peptides at 60°C and 75% relative humidity for 4 hours produces a glycopeptide complex with a molecular weight of 1500 Da (confirmed by mass spectrometry).

[0066] In vitro microbial culture: After anaerobic culture of human fecal microbiota for 24 hours, the number of Bifidobacteria increased by 3.2 times (compared to 1.5 times in the control group).

[0067] Simulated digestion: The release of glutamine after gastrointestinal digestion was determined to be 95% by USP dissolution method (HPLC detection).

[0068] Example 2: Application of Effervescent Granules

[0069] Formula and process:

[0070] Take 72g of protein powder, 10g of citric acid, 13g of sodium bicarbonate, and 3g of hydroxypropyl methylcellulose from Example 1;

[0071] Dry granulation (pressure 12kN), tableting (5g per tablet), and coating with hydroxypropyl methylcellulose ethanol solution.

[0072] performance:

[0073] Particle size D50 = 650 μm, foaming time in water 22 seconds, resolvable pH 6.2;

[0074] Water vapor transmission rate: 0.08 g / (m 2 ·day), disintegration time 50 seconds.

[0075] Example 3: Sports Recovery Preparation

[0076] Formula and administration:

[0077] Protein powder 85g, coconut water powder 10g, electrolytes 5g (NaCl:KCl = 3.75g:1.25g);

[0078] Take 20g (containing 17g of protein powder) 30 minutes after exercise.

[0079] characteristic:

[0080] Reconstituted at 40℃ for 90 seconds, osmotic pressure 265 mOsm / kg (measured by freezing point osmometer).

[0081] Example 4: Application in improving leaky gut syndrome

[0082] Solution and Results:

[0083] Patients with leaky gut syndrome should take 25g daily (divided into two doses) for 21 consecutive days.

[0084] Serum DAO enzyme activity decreased by 52%, fecal calprotectin was 42 μg / g, and ZO-1 protein expression increased by 2.1 times (verified by Western blot).

[0085] Example 5: Yogurt Fermentation Promoter

[0086] Processes and Products:

[0087] Add 4% of Example 1 protein powder (containing 1.6g / L of post-biotic powder) to fresh milk.

[0088] Fermentation at 42℃ for 5.5 hours resulted in a viable cell count of 1.2 × 10⁻⁶. 10 CFU / mL, water holding capacity 87%.

[0089] pH 4.3, acetaldehyde content 28 mg / kg (GC-MS determination).

[0090] Comparative Example 1

[0091] Adjustments: The enzymatic hydrolysis stage was replaced with a single neutral protease (without trypsin), the average molecular weight of the peptides was 850 Da, the proportion of γ-glutamylglutamine dipeptide was 3.1%, and the Maillard reaction step was omitted. All other parameters remained the same as in Example 1.

[0092] result:

[0093] Bifidobacteria increased only 1.8 times in in vitro bacterial culture;

[0094] Simulated digestion resulted in 78% glutamine release.

[0095] Yogurt fermentation time extended to 7 hours, live bacteria count 8×10⁶ 9 CFU / mL.

[0096] Comparative Example 2

[0097] Adjustment:

[0098] The ultrasonic crushing power was 400W, the post-genetic material particle size D90 was 3.2μm, the β-glucan exposure rate was 45%, the spray drying inlet air temperature was 65℃, and the powder moisture content was 4.0%. The remaining parameters were the same as in Example 1.

[0099] result:

[0100] Serum DAO enzyme activity decreased by only 25% in patients with leaky gut syndrome;

[0101] The effervescent granules were reconstituted at pH 5.8, and the disintegration time exceeded 2 minutes.

[0102] Table 1: Performance Comparison Table

[0103]

[0104] Note: Comparative Example 1 did not undergo application testing for leaky gut syndrome; the data are simulated predicted values.

[0105] Application effect correlation:

[0106] 1. A post-biotic β-glucan exposure rate >80% (Example 1: 82%) is directly associated with increased ZO-1 protein expression in leaky gut syndrome;

[0107] 2. The molecular weight (1500 Da) of the Maillard reaction product is in the range of 500-2000 Da, which significantly improves the stability of prebiotics.

[0108] The bar chart comparing the fold increase of Bifidobacteria is shown below. Figure 1 The comparison of serum DAO enzyme activity decrease rates is shown in the bar chart. Figure 2 .

[0109] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and its improved concepts, should be covered within the scope of protection of the present invention.

Claims

1. A method for preparing a high affinity glutamine gut flora modulator protein powder, characterized by, Comprises by mass percentage: 62-68% enzymatic whey protein peptides: 90wt% peptide segments with molecular weight <1kDa, average molecular weight 600±50Da, wherein γ-glutamyl glutamine dipeptide accounts for ≥6%, hydrophobic amino acids account for ≤12%; 19-21% acetylated glutamine: acetylation degree ≥98%, free glutamine residue ≤0.5%; 10-14% complex prebiotics: fructooligosaccharide purity ≥95%, resistant dextrin solubility ≥85%, mass ratio of fructooligosaccharide, resistant dextrin and galactooligosaccharide is 1:1.2:0.5, and further contains 0.3%-0.5% polydextrose; 3-5% inactivated Lactobacillus plantarum postbiotic powder: inactivated at 121℃ for 15min and broken by 800W ice bath ultrasonic for 10min, particle size D90≤1.5μm, exopolysaccharide ≥150mg / g, β-glucan exposure rate ≥80%, short-chain fatty acid retention rate ≥90%; The preparation steps of the high-affinity glutamine intestinal flora modulating protein powder include: (a) Enzymolysis: complex protease with whey protein mass ratio of 2.5% is used for hydrolyzing whey protein at pH 7.0±0.2 and 58℃ for 50min, and the target peptide is obtained by separating through a 10kDa ultrafiltration membrane; (b) Modification: the enzymolysis peptide is mixed with acetylated glutamine at a mass ratio of 3.2:1, 0.25% glutamine transaminase is added, and the reaction is carried out at 42℃ and pH 6.9 for 2.5h to form ε-(γ-glutamyl) lysine isopeptide bond; (c) Postbiotic treatment: Lactobacillus plantarum cells are inactivated at 121℃ for 15min, then broken by 800W ice bath ultrasonic for 10min, and then freeze-dried to obtain powder; (d) Compounding: the product of step (b), complex prebiotics and postbiotic powder are mixed in a nitrogen environment with oxygen content ≤0.1% for 15min; (e) Drying: spray drying, inlet air temperature 58℃, outlet air temperature 33℃, control the water content of the powder ≤3.2%, particle size D50=18±2μm.

2. The method of claim 1, wherein the high affinity glutamine enteric flora modulator powder is prepared by the steps of: a) providing a mixture of a high affinity glutamine enteric flora modulator and a carrier; b) drying the mixture; and c) milling the dried mixture to a desired particle size. 5 The proportion of peptides with 4-7 consecutive hydrophilic amino acids in the enzymatic whey protein peptides is ≥30%, and the proportion of the complex of the enzymatic whey protein peptides and acetylated glutamine is ≥88%.

3. The method for preparing the high-affinity glutamine intestinal flora regulating protein powder according to claim 1, characterized in that: The galactooligosaccharide and polydextrose in the complex prebiotics are subjected to Maillard reaction in the presence of whey protein peptides at 60℃ and relative humidity of 75% for 4h to generate glycopeptide complexes with molecular weight of 500-2000Da.

4. A effervescent granule containing the high-affinity glutamine intestinal flora modulating protein powder prepared by the preparation method of any one of claims 1-3, characterized in that: Composition: Protein powder 70-75%, effervescent system composed of citric acid and sodium bicarbonate, mass ratio of citric acid to sodium bicarbonate 1:1.3, hydroxypropyl methylcellulose coating layer 3%; Performance: particle size D50=500-800μm, water foaming time ≤25s, reconstituted pH 6.0-6.5, water vapor transmission rate ≤0.1g / (m²·day); Process: dry granulation and compression with pressure 10-15 kN, single tablet weight 5 g, disintegration time ≤1 min.

5. Use of the high-affinity glutamine intestinal flora modulator protein powder prepared by the preparation method of any one of claims 1-3 in the preparation of a post-exercise recovery preparation, characterized in that: The compound formula is composed of protein powder, coconut water powder and electrolyte, and the mass ratio of the three is 85:10:5; the electrolyte is a mixture of sodium chloride and potassium chloride, and the mass ratio of sodium chloride to potassium chloride is 3:1; Physical properties: reconstitution time ≤2 min at 40°C, osmotic pressure 270±20 mOsm / kg; Dosage regimen: the single dose of the preparation is 20 g, containing 17 g of protein powder, which is taken within 30 minutes after exercise.

6. Use of the high-affinity glutamine intestinal flora modulator protein powder prepared by the preparation method of any one of claims 1-3 in the preparation of a functional food for improving intestinal barrier function, characterized in that: Dosage regimen: 25 g per day, twice a day, for 21 consecutive days; Biomarker detection: the patient's serum DAO enzyme activity decreases by ≥45%, fecal calprotectin is ≤50 μg / g, and tight junction protein ZO-1 expression increases by 2 times; Structural characteristics: the β-glucan exposure rate of the probiotic powder is ≥80%.

7. Use of the high-affinity glutamine intestinal flora modulator protein powder prepared by the preparation method of any one of claims 1-3 as a yogurt fermentation accelerator, characterized in that: Addition amount: the addition amount is 4% of the mass of the milk base, and the probiotic powder accounts for 4% of the mass of the protein powder; Fermentation conditions: fermentation at 42°C for ≤ 6 hours, resulting in a live bacterial count ≥ 1 x 10 10 CFU / mL, and a water holding capacity ≥ 85%; Product characteristics: the finished yogurt has a pH of 4.2-4.5, and the flavor substance acetaldehyde content is ≥25 mg / kg.

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