Anti-wrinkle composition containing red ginseng extract and polypeptide as well as preparation method and application of anti-wrinkle composition

Abstract

The invention relates to an anti-wrinkle composition containing red ginseng extract and polypeptide as well as a preparation method and application of the anti-wrinkle composition. The anti-wrinkle composition comprises a red ginseng extract, normal-temperature polypeptides, a normal-temperature solvent, high-temperature polypeptides (palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7 and palmitoyl tripeptide-5) and a high-temperature solvent (pentanediol and butanediol). By using the high-temperature solvent, the solubility and stability of part of polypeptide are improved, low-temperature addition and use are facilitated, the activity of the polypeptide can be preserved, and the problems that the polypeptide component is poor in solubility and difficult to add in a cosmetic formula are solved; and the defect that the two components in the prior art cannot effectively cooperate with each other is overcome, so that the overall effect of an anti-wrinkle product is improved.

Description

Technical Field

[0001] The present invention belongs to the field of cosmetics, and in particular relates to an anti-wrinkle composition containing red ginseng extract and polypeptide, and a preparation method and application thereof. Background Art

[0002] Currently, the common anti-wrinkle cosmetics on the market mainly include the following types: antioxidant products containing plant extracts, anti-wrinkle products containing peptides, and few products that combine the two. At the same time, these products have some problems in practical application:

[0003] Problems with the application of peptides in cosmetics: As an effective anti-wrinkle ingredient, peptides generally have low solubility in water and require high temperatures (75°C and above) to dissolve in solvents such as polyols. Therefore, the addition of peptides to cosmetic formulas often faces the risk of difficulty dissolving at low temperatures and easy inactivation at high temperatures, which limits their application in cosmetic formulations. Therefore, how to solve the solubility problem while ensuring the activity of peptides is a major challenge facing the current cosmetics industry.

[0004] Applications of Red Ginseng Extract: Red ginseng extract is rich in various active ingredients, such as polysaccharides, saponins, and flavonoids. These ingredients possess significant antioxidant properties, effectively combating free radicals and delaying skin aging. However, existing applications of red ginseng extract in anti-wrinkle cosmetics primarily focus on its single antioxidant effect, lacking effective combination with other anti-wrinkle ingredients, such as peptides, to achieve a synergistic effect.

[0005] In summary, existing anti-wrinkle cosmetics have deficiencies in terms of the solubility and activity protection of polypeptides, and the synergistic effect of red ginseng extract and polypeptides. There is an urgent need for a new anti-wrinkle composition that can solve these problems. Summary of the Invention

[0006] The purpose of the present invention is to address the problems in the prior art and provide an anti-wrinkle composition containing red ginseng extract and polypeptide, as well as a preparation method and application thereof.

[0007] The present invention solves the problem of poor solubility and difficulty in adding polypeptide ingredients to cosmetic formulas by adopting a composition form that is easy to add and use at low temperatures and can preserve the activity of polypeptides. At the same time, it innovatively combines red ginseng extract with polypeptides to produce a synergistic effect in anti-wrinkle efficacy, overcoming the defect of the existing technology that the two cannot effectively cooperate with each other, thereby improving the overall effect of anti-wrinkle products.

[0008] The purpose of the present invention can be achieved by the following solutions:

[0009] The present invention provides an anti-wrinkle composition containing red ginseng extract and polypeptide, comprising the following components in the following mass fractions:

[0010] Red ginseng extract 0.60%-3%,

[0011] Room temperature solvent 3.78%-19%,

[0012] Room temperature polypeptide 0.02%-0.10%,

[0013] High temperature peptide 0.03%-0.15%,

[0014] High temperature solvent 78%-95%;

[0015] The high-temperature peptide includes one or more of palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5;

[0016] The high-temperature solvent includes one or more of pentanediol and butanediol.

[0017] The invention comprises a room temperature component A and a high temperature component B. Component A comprises red ginseng extract, a room temperature solvent and a room temperature polypeptide; component B comprises a high temperature polypeptide and a high temperature solvent.

[0018] As one embodiment of the present invention, the normothermic polypeptide includes one or more of acetyl hexapeptide-8 and carnosine;

[0019] As one embodiment of the present invention, the normal temperature solvent includes water.

[0020] As an embodiment of the present invention, the amount of red ginseng extract is preferably 0.60%-1%.

[0021] As one embodiment of the present invention, the high temperature polypeptide is preferably palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5. Such polypeptides are difficult to dissolve at low temperatures and are easily inactivated by high temperature treatment. The high temperature polypeptide is preferably 0.01%-0.05% palmitoyl pentapeptide-4, 0.01%-0.05% palmitoyl tetrapeptide-7, and 0.01%-0.05% palmitoyl tripeptide-5, and more preferably 0.01%-0.02% palmitoyl pentapeptide-4, 0.01%-0.02% palmitoyl tetrapeptide-7, and 0.01%-0.02% palmitoyl tripeptide-5.

[0022] In one embodiment of the present invention, the high-temperature solvent is preferably pentylene glycol or butylene glycol. The high-temperature solvent is preferably 10%-30% pentylene glycol and 55%-75% butylene glycol, more preferably 15%-25% pentylene glycol and 65%-75% butylene glycol. The combination of pentylene glycol and butylene glycol, when heated (50°C-60°C), dissolves the high-temperature polypeptide component, improves polypeptide stability, prevents polypeptide inactivation, and imparts a pleasant skin feel to the composition.

[0023] As one embodiment of the present invention, the room-temperature polypeptide is preferably acetyl hexapeptide-8 or carnosine. The room-temperature polypeptide is preferably acetyl hexapeptide-8 0.01%-0.05% and carnosine 0.01%-0.05%, more preferably acetyl hexapeptide-8 0.01%-0.02% and carnosine 0.01%-0.02%.

[0024] The red ginseng extract of the present invention serves as the primary functional ingredient, providing antioxidant activity. The ambient temperature solvent in component A acts as a base solvent, providing a basic moisturizing effect for the skin. The polypeptides used in the present invention include acetyl hexapeptide-8 (which inhibits neurotransmitters), palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, palmitoyl tripeptide-5, and carnosine. Each of these peptides has a specific anti-wrinkle mechanism. For example, acetyl hexapeptide-8 can inhibit the release of neurotransmitters, thereby reducing dynamic lines caused by muscle contraction; palmitoyl pentapeptide-4 can promote the synthesis of type I, type III, type IV collagen and elastin, and inhibit the degradation of collagen by matrix metalloproteinases (MMPs); palmitoyl tetrapeptide-7 can inhibit the secretion of cellular inflammatory factors (IL-6), promote the synthesis of laminin, fibronectin, and collagen, and reduce skin wrinkles; palmitoyl tripeptide-5 can promote the production of collagen and elastin, inhibit ultraviolet damage to cells, inhibit the activity of matrix metalloproteinases (MMPs), reduce collagen degradation, and have firming and wrinkle-removing effects; carnosine can react with reducing sugars, competitively inhibit non-enzymatic glycosylation reactions, inhibit the formation of glycation end products, prevent abnormal cross-linking of extracellular matrix collagen, reduce pigmentation, maintain skin barrier function, and delay and improve skin aging symptoms.

[0025] The present invention also provides a method for preparing the anti-wrinkle composition containing red ginseng extract and polypeptide, comprising the following steps:

[0026] S1. Mixing and stirring the red ginseng extract, the room temperature polypeptide, and the room temperature solvent to form a component A solution;

[0027] S2. Mixing the high-temperature polypeptide component with the high-temperature solvent and heating to fully dissolve the polypeptide to obtain a component B solution;

[0028] S3. Mixing the prepared component A solution and component B solution to obtain the anti-wrinkle composition containing red ginseng extract and polypeptide.

[0029] As one embodiment of the present invention, in step S1, the red ginseng extract, room-temperature polypeptide, and room-temperature solvent are stirred at room temperature (20°C-25°C) to form a uniform solution of component A. This process is simple to operate, does not require high-temperature treatment, and helps save energy.

[0030] As an embodiment of the present invention, in step S2, the heating temperature is 50°C-60°C, preferably 50°C-55°C. Heat slowly and control the appropriate temperature. The specific temperature can be determined according to the properties of the solvent and the polypeptide, generally around 50°C-60°C, so that the polypeptide is fully dissolved. Inactivation is a change in the spatial configuration of the polypeptide caused by temperature. The dissolved polypeptide (the undissolved polypeptide will not be inactivated, but its efficacy characteristics will not be exerted) can basically maintain its activity below 50°C. It will gradually lose its activity when the temperature rises, and irreversible changes will occur above 60°C. This method of heating and dissolving the polypeptide of the present invention can effectively solve the problem of poor solubility of the polypeptide component. At the same time, by controlling the temperature, the high-temperature solvent can stabilize the spatial configuration of the polypeptide and avoid the damage of high temperature to the activity of the polypeptide.

[0031] As an embodiment of the present invention, in step S3, the mixing of component A solution and component B solution is carried out at 40°C-45°C. The prepared component A and component B are mixed, and after the mixed and dissolved component B is completely cooled to lukewarm (40°C-45°C), they are fully stirred to uniformly mix the two, thereby obtaining a final anti-wrinkle composition containing red ginseng extract and polypeptides. During the mixing process, it is necessary to ensure that component A and component B are fully mixed to achieve a good synergistic effect. Polyols (such as butanediol, pentanediol, etc.) contain multiple hydroxyl groups, which can combine with water molecules and polar groups of polypeptides through hydrogen bonds to form a stable solvation layer. At high temperatures, the hydrogen bonding effect is enhanced, which promotes the dissolution of polypeptides; after cooling, the hydrogen bond network still maintains the hydrophilic environment of the polypeptide, inhibiting precipitation caused by hydrophobic aggregation, so there will be no precipitation of polypeptides after cooling.

[0032] The present invention can be applied to products such as cleansing honey, essence water, essence liquid, essence milk, essence cream, etc., and provides an application of the anti-wrinkle composition containing red ginseng extract and polypeptide in essence water.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) Solving the problem of polypeptide solubility: By pre-mixing and dissolving the polypeptide with the solvent component to prepare component B, the solubility of the polypeptide in the composition is significantly improved. This not only facilitates the addition operation during the production process and avoids problems such as low production efficiency caused by the difficulty of dissolving the polypeptide, but also allows addition and mixing at low temperatures, effectively preserving the biological activity of the polypeptide, thereby ensuring the anti-wrinkle effect of the product.

[0035] (2) Synergistic Anti-Wrinkle Effect: This invention innovatively combines red ginseng extract with multiple peptides, leveraging the antioxidant properties of red ginseng and the targeted anti-wrinkle mechanism of the peptides to achieve synergistic anti-wrinkle efficacy. Experimental data show that the combined anti-wrinkle effect is significantly superior to that of a single ingredient, more effectively increasing COL5A1 gene expression, thereby reducing wrinkle formation and delaying skin aging.

[0036] (3) Good safety and stability: The red ginseng extract, peptides, and various solvent components selected all have good safety characteristics and comply with relevant standards and regulatory requirements for cosmetic raw materials. Furthermore, through reasonable formulation design and preparation process, the various components in the composition can coexist stably, making it less susceptible to deterioration or loss of active ingredients. This extends the product's shelf life and provides consumers with a safer and more reliable choice. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Other features, objects and advantages of the present invention will become more apparent upon reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0038] Figure 1 The anti-wrinkle effect of the subjects in Example 1 after using the essence water for 28 days (the left picture is D0, the right picture is D28);

[0039] Figure 2 This is the appearance of the composition of Example 1. DETAILED DESCRIPTION

[0040] The present invention is described in detail below in conjunction with specific embodiments. The following embodiments are implemented under the premise of the technical solution of the present invention, provide detailed implementation methods and specific operating procedures, and will help those skilled in the art to further understand the present invention. It should be pointed out that the protection scope of the present invention is not limited to the following embodiments, and several adjustments and improvements made under the premise of the concept of the present invention all fall within the protection scope of the present invention.

[0041] The anti-wrinkle composition of the present invention achieves excellent anti-wrinkle effects through the following synergistic mechanisms:

[0042] (1) Synergistic effect of antioxidant and anti-wrinkle: Red ginseng extract enhances the skin's barrier function through its powerful antioxidant effect, keeping the skin in a healthier state and facilitating better penetration of polypeptide ingredients into the dermis.

[0043] (2) Targeted effect: The polypeptide components target the key pathways of wrinkle formation, complementing the antioxidant effect of red ginseng to jointly exert a more significant anti-wrinkle effect.

[0044] (3) Low concentration and high efficiency: This synergistic effect not only enhances the anti-wrinkle efficacy of a single ingredient, but also enables the composition to achieve better anti-wrinkle effects at lower concentrations, thereby improving the safety and economy of the product.

[0045] Attached is the manufacturer of raw materials used in the present invention:

[0046] Red ginseng extract: Shaanxi Huatai Biological Fine Chemical Co., Ltd.;

[0047] Centella asiatica extract: Shanghai Jiakai Biotechnology Co., Ltd.

[0048] Portulaca oleracea extract: Shanghai Zhina Biotechnology Co., Ltd.;

[0049] Deionized water: Shanghai Linqingxuan Biotechnology Co., Ltd.;

[0050] Glycerol: Emery Oleochemicals (M) Sdn Bhd;

[0051] Butanediol: KH Neochem., Ltd.;

[0052] Pentylene glycol: Thor Specialty Chemicals (Zhenjiang) Co., Ltd.

[0053] Carnosine, palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, palmitoyl tripeptide-5, acetyl hexapeptide-8: Shenzhen Readlin Biotechnology Co., Ltd.

[0054] Example 1

[0055] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0056] Component A

[0057] Red ginseng extract: 0.60%,

[0058] Carnosine: 0.01%,

[0059] Acetyl Hexapeptide-8: 0.01%,

[0060] Water: 9.35%,

[0061] Component B

[0062] Palmitoyl Pentapeptide-4: 0.01%,

[0063] Palmitoyl Tetrapeptide-7: 0.01%,

[0064] Palmitoyl Tripeptide-5: 0.01%,

[0065] Pentylene glycol: 20%,

[0066] Butanediol: 70%,

[0067] The preparation method is as follows:

[0068] Mix red ginseng extract, carnosine, acetyl hexapeptide-8 and water, and stir evenly at room temperature (25° C.) to form a component A solution.

[0069] Mix palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5 with pentanediol and butylene glycol, and heat to 50°C to fully dissolve the polypeptides to obtain component B solution.

[0070] Component B is cooled to 45°C, and component A is added and stirred thoroughly to mix the two components evenly to obtain the final anti-wrinkle composition containing red ginseng extract and polypeptide. Figure 2 shown.

[0071] Example 2

[0072] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0073] Component A

[0074] Red ginseng extract: 3%

[0075] Carnosine: 0.05%

[0076] Acetyl Hexapeptide-8: 0.05%

[0077] Water: 6.75%

[0078] Component B

[0079] Palmitoyl Pentapeptide-4: 0.05%

[0080] Palmitoyl Tetrapeptide-7: 0.05%

[0081] Palmitoyl Tripeptide-5: 0.05%

[0082] Pentylene glycol: 20%

[0083] Butanediol: 70%

[0084] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0085] The preparation method is the same as that of Example 1.

[0086] Comparative Example 1

[0087] Component A

[0088] Red ginseng extract: 0.60%

[0089] Carnosine: 0.01%

[0090] Acetyl Hexapeptide-8: 0.01%

[0091] Water: 9.35%

[0092] Component B

[0093] Palmitoyl Pentapeptide-4: 0.01%

[0094] Palmitoyl Tetrapeptide-7: 0.01%

[0095] Palmitoyl Tripeptide-5: 0.01%

[0096] Pentylene glycol: 20%

[0097] Glycerin: 70%

[0098] The preparation method is the same as Example 1, but component B cannot be fully dissolved.

[0099] Comparative Example 2

[0100] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0101] Component A

[0102] Red ginseng extract: 0.60%

[0103] Carnosine: 0.01%

[0104] Acetyl Hexapeptide-8: 0.01%

[0105] Water: 9.35%

[0106] Component B

[0107] Palmitoyl Pentapeptide-4: 0.01%

[0108] Palmitoyl Tetrapeptide-7: 0.01%

[0109] Palmitoyl Tripeptide-5: 0.01%

[0110] Glycerin: 20%

[0111] Butanediol: 70%

[0112] The preparation method was the same as in Example 1, but component B could not be fully dissolved.

[0113] Comparative Example 3

[0114] Component A

[0115] Centella asiatica extract: 0.60%

[0116] Carnosine: 0.01%

[0117] Acetyl Hexapeptide-8: 0.01%

[0118] Water: 9.35%

[0119] Component B

[0120] Palmitoyl Pentapeptide-4: 0.01%

[0121] Palmitoyl Tetrapeptide-7: 0.01%

[0122] Palmitoyl Tripeptide-5: 0.01%

[0123] Pentylene glycol: 20%

[0124] Butanediol: 70%

[0125] The preparation method is as follows:

[0126] Mix the Centella asiatica extract, carnosine, acetyl hexapeptide-8 and water, and stir evenly at room temperature (25° C.) to form a component A solution.

[0127] Mix palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5 with pentanediol and butylene glycol, and heat to 50°C to fully dissolve the polypeptides to obtain component B solution.

[0128] Component B is cooled to 45° C., and component A is added and stirred thoroughly to uniformly mix the two components to obtain the final anti-wrinkle composition containing Centella asiatica extract and polypeptide.

[0129] Comparative Example 4

[0130] An anti-wrinkle composition containing purslane extract and polypeptide is prepared, and its components are as follows:

[0131] Component A

[0132] Portulaca oleracea extract: 0.60%

[0133] Carnosine: 0.01%

[0134] Acetyl Hexapeptide-8: 0.01%

[0135] Water: 9.35%

[0136] Component B

[0137] Palmitoyl Pentapeptide-4: 0.01%

[0138] Palmitoyl Tetrapeptide-7: 0.01%

[0139] Palmitoyl Tripeptide-5: 0.01%

[0140] Pentylene glycol: 20%

[0141] Butanediol: 70%

[0142] The preparation method is as follows:

[0143] Mix the purslane extract, carnosine, acetyl hexapeptide-8 and water, and stir them evenly at room temperature (25° C.) to form a component A solution.

[0144] Mix palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5 with pentanediol and butylene glycol, and heat to 50°C to fully dissolve the polypeptides to obtain component B solution.

[0145] Component B is cooled to 45° C., and component A is added and stirred thoroughly to uniformly mix the two components to obtain the final anti-wrinkle composition containing purslane extract and polypeptide.

[0146] Comparative Example 5

[0147] An anti-wrinkle composition containing a polypeptide is prepared, the components of which are as follows:

[0148] Component A

[0149] Carnosine: 0.01%

[0150] Acetyl Hexapeptide-8: 0.01%

[0151] Water: 9.95%

[0152] Component B

[0153] Palmitoyl Pentapeptide-4: 0.01%

[0154] Palmitoyl Tetrapeptide-7: 0.01%

[0155] Palmitoyl Tripeptide-5: 0.01%

[0156] Pentylene glycol: 20%

[0157] Butanediol: 70%

[0158] The preparation method is as follows:

[0159] Mix carnosine, acetyl hexapeptide-8 and water, and stir evenly at room temperature (25°C) to form a component A solution.

[0160] Mix palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5 with pentanediol and butylene glycol, and heat to 50°C to fully dissolve the polypeptides to obtain component B solution.

[0161] Component B was cooled to 45°C, and component A was added and stirred thoroughly to uniformly mix the two components to obtain the final composition.

[0162] Comparative Example 6

[0163] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0164] Component A

[0165] Red ginseng extract: 0.65%

[0166] Water: 9.35%

[0167] Component B

[0168] Pentylene glycol: 20%

[0169] Butanediol: 70%

[0170] The preparation method is as follows:

[0171] The red ginseng extract, water, pentanediol and butylene glycol are mixed and stirred at room temperature (25° C.) to form a component composition.

[0172] Comparative Example 7

[0173] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0174] Component A

[0175] Red ginseng extract: 0.60%

[0176] Water: 9.35%

[0177] Component B

[0178] Palmitoyl Pentapeptide-4: 0.016%

[0179] Palmitoyl Tetrapeptide-7: 0.016%

[0180] Palmitoyl Tripeptide-5: 0.018%

[0181] Pentylene glycol: 20%

[0182] Butanediol: 70%

[0183] The preparation method is as follows:

[0184] Mix the red ginseng extract and water, and stir evenly at room temperature (25°C) to form a component A solution.

[0185] Mix palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5 with pentanediol and butylene glycol, and heat to 50°C to fully dissolve the polypeptides to obtain component B solution.

[0186] Component B is cooled to 45° C., and component A is added and stirred thoroughly to uniformly mix the two components to obtain the final anti-wrinkle composition containing red ginseng extract and polypeptide.

[0187] Comparative Example 8

[0188] An anti-wrinkle composition containing red ginseng extract and polypeptide was prepared, and its components were as follows:

[0189] Component A

[0190] Red ginseng extract: 0.60%

[0191] Carnosine: 0.025%

[0192] Acetyl Hexapeptide-8: 0.025%

[0193] Water: 9.35%

[0194] Component B

[0195] Pentylene glycol: 20%

[0196] Butanediol: 70%

[0197] The preparation method is as follows:

[0198] The red ginseng extract, carnosine, acetyl hexapeptide-8 and water are mixed and stirred at room temperature (25° C.), and then pentanediol and butylene glycol are added and mixed to obtain the final anti-wrinkle composition containing red ginseng extract and polypeptide.

[0199] Comparative Example 9

[0200] Component A

[0201] Red ginseng extract: 0.60%

[0202] Carnosine: 0.01%

[0203] Acetyl Hexapeptide-8: 0.01%

[0204] Water: 9.35%

[0205] Component B

[0206] Palmitoyl Pentapeptide-4: 0.01%

[0207] Palmitoyl Tetrapeptide-7: 0.01%

[0208] Palmitoyl Tripeptide-5: 0.01%

[0209] Butanediol: 90%

[0210] The preparation method is the same as Example 1, but component B cannot be fully dissolved.

[0211] Comparative Example 10

[0212] Component A

[0213] Red ginseng extract: 0.60%

[0214] Carnosine: 0.01%

[0215] Acetyl Hexapeptide-8: 0.01%

[0216] Water: 9.35%

[0217] Component B

[0218] Palmitoyl Pentapeptide-4: 0.01%

[0219] Palmitoyl Tetrapeptide-7: 0.01%

[0220] Palmitoyl Tripeptide-5: 0.01%

[0221] Pentylene glycol: 90%

[0222] The preparation method is the same as Example 1, but component B cannot be fully dissolved.

[0223] Comparative Example 11

[0224] The components and preparation method are the same as those in Example 1, except that when preparing the solution of component B, the temperature is heated to 45° C. Component B cannot be fully dissolved.

[0225] Safety tests, efficacy tests, and stability tests were performed on the anti-wrinkle compositions containing red ginseng extract and polypeptides according to the examples of the present invention and the comparative examples.

[0226] (1) Safety testing

[0227] Select appropriate patch equipment and use the closed patch test method. Place approximately 0.020g-0.025g of the test substance in the patch tester, and apply it to the inner arm with low-sensitivity tape. Remove the test substance after 24 hours, and observe the skin reaction 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0228] Table 1 Patch results of 50% concentration samples

[0229]

[0230]

[0231] (2) Cytotoxicity test

[0232] Principle and method:

[0233] The mitochondria of living cells contain succinate dehydrogenase, which can reduce exogenous MTT to water-insoluble, blue-purple crystalline formazan, which is deposited in the cells. Dead cells, however, do not have this function. Dimethyl sulfoxide (DMSO) dissolves the formazan in cells, and the absorbance at a wavelength of 490 nm is measured using a multifunctional microplate reader. Since the amount of crystals formed is proportional to the number of living cells, the absorbance value can indirectly reflect the number of living cells.

[0234] Experimental steps:

[0235] Human dermal fibroblasts were seeded into 96-well plates (5*10 3 Cells were cultured at 37°C, 5% CO₂ for 24 hours. Fresh culture medium containing the corresponding sample concentrations was added to the sample groups, while fresh culture medium was replaced with that for the normal and blank control groups. The cells were incubated at 37°C, 5% CO₂ for another 24 hours. Following incubation, MTT solution was added to each well and cultured for another 4 hours. The culture medium was removed, and DMSO solution was added. The cells were shaken to mix thoroughly, and the absorbance at 490 nm was measured to calculate cell viability.

[0236]

[0237] OD 490 : Absorbance value at 490nm

[0238] Table 2 Cytotoxicity of 1% concentration samples (human dermal fibroblasts)

[0239]

[0240]

[0241] (3) Efficacy test

[0242] The protein encoded by COL5A1 is a key component of type V collagen in the extracellular matrix. It regulates the assembly and diameter of collagen fibrils, influencing tissue mechanical strength and elasticity. Type V collagen is a fibrillar collagen primarily found in tissues such as the dermis and cornea. COL5A1 gene expression levels decrease with age, which may be related to skin aging and collagen fiber reduction. The anti-wrinkle efficacy of the sample was evaluated by measuring the relative increase in COL5A1 gene expression in human dermal fibroblasts at a 0.01% dose concentration after UVA irradiation.

[0243] Experimental steps:

[0244] Human dermal fibroblasts were seeded in 6-well plates (3×10 5 / well) and incubated at 37 ° C, 5% CO2 for 24 hours. After the incubation, the culture medium was removed and the cells were washed twice with D-Hank. Then, the sample group and the model control group were irradiated with UVA (9 J / cm 2 ).

[0245] After modeling, the normal control group and the model control group were replaced with fresh culture medium, and the sample group was added with fresh culture medium containing samples, and incubated at 37°C, 5% CO2 for 24 h.

[0246] Total RNA was extracted from each experimental group, cDNA was synthesized, and the gene expression of β-actin and target genes was detected by q-PCR.

[0247] β-actin was used as an internal reference for gene expression to calculate the relative RNA expression of the target gene.

[0248]

[0249] Q: relative gene expression

[0250] The experimental results are shown in Table 3:

[0251] Table 3 Efficacy test results

[0252]

[0253]

[0254] (4) Stability test:

[0255] The compositions prepared in each example and comparative example were subjected to a two-month stability test. The stability test results are shown in the table below. As can be seen from the results, the compositions prepared under different conditions maintained no color change, and no precipitation, decreased liquid transparency, or unstable demixing occurred, indicating that the stability test passed.

[0256] Table 4 Stability test results (2 months)

[0257] 48℃ -18℃ 40℃ 3℃ High and low temperature cycle Example 1 pass pass pass pass pass Example 2 pass pass pass pass pass Comparative Example 3 pass pass pass pass pass Comparative Example 4 pass pass pass pass pass Comparative Example 5 pass pass pass pass pass Comparative Example 6 pass pass pass pass pass Comparative Example 7 pass pass pass pass pass Comparative Example 8 pass pass pass pass pass

[0258] Application Examples

[0259] The composition is applied to an essence water product, and the formula is shown in Table 5 below:

[0260] Table 5 Essence water composition

[0261]

[0262] Efficacy test results:

[0263] Under test conditions, 31 Chinese subjects with sensitive skin used the product for 28 days.

[0264] 100% of the subjects believed that the product had anti-wrinkle effects;

[0265] 100% of the subjects felt that their skin elasticity had returned;

[0266] 100% of the subjects felt that their skin was more refined and firmer;

[0267] One of the subjects used the essence water for 28 days and the anti-wrinkle effect was as follows: Figure 1As shown (the left picture is D0, the right picture is D28).

[0268] The above describes the specific embodiments of the present invention. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art may make various variations or modifications within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. An anti-wrinkle composition containing red ginseng extract and polypeptide, characterized in that: The following components are included in the mass fraction: Red ginseng extract 0.60%-3%, Room temperature solvent 3.78-19%, Room temperature polypeptide 0.02-0.10%, High temperature peptide 0.03%-0.15%, High temperature solvent 78%-95%; The high-temperature peptide includes one or more of palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5; The high-temperature solvent includes one or more of pentanediol and butanediol.

2. The anti-wrinkle composition according to claim 1, characterized in that Normal temperature polypeptides include one or more of acetyl hexapeptide-8 and carnosine; And / or, the ambient temperature solvent includes water.

3. The anti-wrinkle composition according to claim 1, characterized in that High-temperature peptides include palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, and palmitoyl tripeptide-5.

4. The anti-wrinkle composition according to claim 1, characterized in that High temperature solvents include pentanediol and butylene glycol.

5. The anti-wrinkle composition according to claim 1, wherein Normal temperature peptides include acetyl hexapeptide-8 and carnosine.

6. A method for preparing the anti-wrinkle composition according to claim 1, characterized in that: The steps include: S1. Mixing and stirring the red ginseng extract, the room temperature polypeptide, and the room temperature solvent to form a component A solution; S2. Mixing the high-temperature polypeptide component with the high-temperature solvent and heating to fully dissolve the polypeptide to obtain a component B solution; S3. Mixing the prepared component A solution and component B solution to obtain the anti-wrinkle composition containing red ginseng extract and polypeptide.

7. The method for preparing the anti-wrinkle composition according to claim 6, wherein: In step S1, the red ginseng extract, the room-temperature polypeptide and the room-temperature solvent are stirred at room temperature to form a component A solution.

8. The method for preparing the anti-wrinkle composition according to claim 6, wherein: In step S2, the heating temperature is 50-60°C.

9. The method for preparing the anti-wrinkle composition according to claim 6, wherein: In step S3, the component A solution and the component B solution are mixed at 40°C-45°C.

10. Use of the anti-wrinkle composition according to claim 1 in preparing cleansing honey, essence water, essence liquid, essence lotion or essence cream.

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