Temperature and humidity control reaction cabin for inkjet printing enzymatic DNA synthesis

Abstract

The invention discloses a temperature and humidity control reaction cabin for inkjet printing enzymatic DNA synthesis, which integrates a cabin gas inlet connecting part, a reaction area, a slidable upper cover and a chip fixing cover plate, and the cabin gas inlet connecting part ensures that gas with certain temperature and humidity is uniformly introduced into the reaction area through a plurality of rows of parallel or crossed gas inlet holes; a DNA synthesis chip is placed in the reaction area, and stable clamping of the DNA synthesis chip is guaranteed in cooperation with a chip fixing cover plate; the slidable upper cover is matched with the ink-jet printing head, when the ink-jet printing head is used for distributing a synthesis reagent to the surface of the DNA synthesis chip, the upper cover leaves a reaction area, and when DNA enzymatic synthesis reaction is carried out after the reagent distribution is completed, the upper cover covers the reaction area again, so that the synthesis reaction area keeps a relatively stable temperature and humidity gas environment; the reaction chamber can effectively control the surface temperature and humidity of the DNA synthesis chip, meet the reaction conditions of enzymatic DNA synthesis, and control the temperature and humidity environment of a plurality of chips at the same time.

Description

Technical Field

[0001] The present invention relates to the field of inkjet printing DNA synthesis, in particular to a temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis. Background Art

[0002] DNA synthesis technology is a bottleneck technology in the development of synthetic biology in my country. At present, the methods for synthesizing DNA on the market are still mainly the first-generation chemical synthesis proposed in the last century. However, the waste liquid generated by chemical DNA synthesis is toxic and the amount of waste liquid is large, which places high demands on laboratory safety protection and waste liquid treatment, and is likely to have a certain impact on the environment. At present, the second-generation enzymatic DNA synthesis mediated by template-independent terminal deoxynucleotidyl transferase (TdT enzyme) is developing rapidly. Compared with the first-generation chemical synthesis technology, the synthesis reagent of the second-generation enzymatic DNA synthesis is an aqueous solution, which has the advantages of high synthesis efficiency, mild reaction conditions, and low environmental pollution. At present, enzymatic DNA synthesis usually completely immerses the synthesis carrier such as magnetic beads in the liquid phase synthesis reagent, and the synthesis temperature needs to be controlled to ensure that the reaction rate of the enzyme reaches the optimal level.

[0003] Enzymatic DNA synthesis based on inkjet printing can automate enzymatic DNA synthesis, further reducing the consumption of enzyme and monomer reagents, enabling high-throughput DNA synthesis and significantly promoting the development of enzymatic DNA synthesis. However, currently, enzymatic DNA synthesis technology based on inkjet printing is still in its infancy both domestically and internationally. When using inkjet printing technology to distribute synthesis reagents, because the inkjet-printed droplets are small and easily evaporated, the humidity of the DNA synthesis environment must be strictly controlled to reduce droplet evaporation, maintain the concentration of the reaction reagents in the droplets, and successfully complete the enzymatic DNA synthesis reaction. At the same time, to ensure optimal enzyme reactivity, the temperature of the DNA synthesis environment must also be controlled to improve the reaction efficiency of enzymatic DNA synthesis. If the temperature and humidity are controlled using a glove box equipped with a chemical DNA synthesis inkjet printer, the glove box is too large to meet the temperature and humidity control requirements for enzymatic DNA synthesis. Excessive humidity in the glove box can also damage the inkjet printing device. Currently, there is a lack of miniaturized, automated devices that can efficiently and stably control the temperature and humidity of DNA synthesis chips for enzymatic DNA synthesis based on inkjet printing technology. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis.

[0005] In order to solve the above technical problems, the technical solutions proposed in the present invention are as follows:

[0006] A temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, comprising a chamber air inlet connection 101, a first reaction area 102, a second reaction area 103, a slidable upper cover 104 and a chip fixing cover 105. The chamber air inlet connection 101 is fixed between the first reaction area 102 and the second reaction area 103, the slidable upper cover 104 is placed above the chamber air inlet connection 101, the first reaction area 102 and the second reaction area 103 and is slidably connected, and the chip fixing cover 105 is arranged in the first reaction area 102 and the second reaction area 103.

[0007] The above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, combined with a humidity generator and other devices to provide gas with a certain humidity and temperature, can control the stability of the temperature and humidity in the reaction chamber, provide environmental conditions for the synthesis of inkjet printing enzymatic DNA, and effectively promote the further development of enzymatic DNA synthesis and its automation.

[0008] Preferably, the temperature and humidity control reaction chamber for inkjet printing enzymatic DNA synthesis, the chamber air inlet connection part 101 includes a two-position three-way solenoid valve 201, a gas conduit 202, an air inlet chamber 203 and an air inlet connection plate 204, a partition 205 is provided in the middle of the air inlet chamber 203, and the air inlet chamber 203 is divided into a left chamber and a right chamber by the partition 205, the air inlet chamber 203 is arranged between the first reaction area 102 and the second reaction area 103, the left chamber is connected to the first reaction area 102 through the air inlet connection plate 204 on one side thereof. The reaction area 102 is fixedly connected, and the right chamber is fixedly connected to the second reaction area 103 through an air inlet connecting plate 204 on one side thereof. The air inlet connecting plate 204 is symmetrically arranged on both sides of the air inlet chamber 203. The two-position three-way solenoid valve 201 includes an air inlet channel and two air outlet channels. The three channels are respectively connected to the humidity generator 5 and the left chamber and the right chamber of the air inlet chamber 203 through the gas duct 202. The two-position three-way solenoid valve is used to control the gas from the humidity generator 5 to be connected to only one side of the air inlet chamber 203 at the same time.

[0009] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the main structure of the air inlet chamber 203 is a rectangular chamber structure, and two circular holes for connecting the gas duct 202 are opened on the front wall of the chamber. The partition 205 arranged in the middle of the chamber is used to buffer the gas to ensure that the gases in the chambers on both sides are not connected to each other, while being connected to their respective synthesis reaction areas; the air inlet connecting plate is a rectangular plate with a rectangular through hole in the middle, and the size of the through hole matches the external size of the air inlet chamber. The air inlet connecting plate can be sealed and fixed to the peripheral parts on both sides of the air inlet chamber by gluing to complete the assembly of the cabin air inlet connecting part body; the air inlet connecting plate 204 and the first reaction area 102 / the second reaction area 103 are sealed and connected by a clamping gasket to ensure air tightness.

[0010] Preferably, in the above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the air inlet connecting plate 204 is a separate rectangular structure, which is installed on both sides of the air inlet chamber 203. The size of the rectangular through hole hollowed out in the middle of the air inlet connecting plate 204 is the same as the size of the air inlet chamber 203, so that the air inlet connecting plate 204 can be accurately inserted into both sides of the air inlet chamber 203 and sealed and fixed. The air inlet connecting plate 204 and the first reaction area 102 / the second reaction area 103 are tightly assembled by clamping gaskets.

[0011] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the first reaction area 102 / the second reaction area 103 is a dustpan-shaped structure, including a hole array connecting wall 301, an upper cover supporting side wall 302, and a chamber lower wall composed of a chip placement platform 303 and a bottom slope 304. The hole array connecting wall 301 is respectively located at one end of the bottom slope 304 of the chamber lower wall, and the upper cover supporting side wall 302 is symmetrically arranged on both sides of the bottom slope 304 of the chamber lower wall and fixedly connected to both sides of the hole array connecting wall 301. The bottom slope 304 is adjacent to the upper cover supporting side wall 302, and a chip placement platform 303 for supporting and fixing the DNA synthesis chip is respectively provided. The thickness of the bottom slope 304 at the connection position with the hole array connecting wall 301 gradually decreases in the direction away from the hole array connecting wall 301, so that a downward inclined angle is formed between the bottom slope 304 and the horizontal plane.

[0012] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the hole array connecting wall 301 is located at one end of the dustpan-shaped structure, and the middle part of the hole array connecting wall 301 has arrayed air inlet holes arranged in a parallel or cross pattern. The diameter of each air inlet hole is the same, and the hole center distances between adjacent air inlet holes in each horizontal row and each vertical row are equal to ensure that the air flow flows into the reaction area evenly and stably.

[0013] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, a long strip slide rail is provided above the upper cover supporting side wall 302, and its cross section is an isosceles triangle, which cooperates with the triangular recess of the slidable upper cover 104 to support the upper cover, ensuring smooth sliding of the upper cover between the first reaction area 102 and the second reaction area 103.

[0014] Preferably, the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis is characterized in that the lower wall of the chamber is composed of a chip placement platform 303 and a bottom inclined surface 304. The width of the lower wall of the chamber is equal to the length of the hole array connecting wall 301 minus the thickness of the upper cover support side walls 302 on both sides. A chip placement platform 303 is provided on the lower wall of the chamber adjacent to the upper cover support side walls for supporting and fixing the DNA synthesis chip; a long boss is provided on each of the two chip placement platforms 303 adjacent to the upper cover support side walls to cooperate with the chip fixing cover 10 5 achieves fixed clamping of the DNA synthesis chip, so that the DNA synthesis chip remains stable during chamber movement, reagent injection, and gas purging; between the chip placement platforms 303 on both sides is a bottom slope 304, and the bottom slope 304 forms a downward angle with the horizontal plane to ensure that the waste liquid generated by the DNA synthesis reaction can flow smoothly along the bottom slope 304 into the waste liquid tank provided below the chamber to achieve waste liquid collection. The bottom slope 304 is coated with a hydrophobic coating to increase the hydrophobicity of the slope, improve the waste liquid collection efficiency, and reduce the risk of waste liquid accumulation.

[0015] Preferably, in the temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis, the chip-fixing cover 105 includes two types: a rectangular cover 501 and a rotatable clamping cover 502. The rectangular cover 501 has a larger contact area with the DNA synthesis chip, enabling more stable support; the rotatable clamping cover 502 facilitates the installation of the DNA synthesis chip, eliminating the need for removal each time.

[0016] Preferably, in the above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the sliding upper cover structure 104 includes an upper cover body 401, an air inlet baffle 402 and an upper cover handle 403, the air inlet baffle 402 is fixed on both sides of the upper cover body 401, the upper cover handle 403 is located on the upper surface of the upper cover body 401, and the upper cover body 401 is provided with an upper cover exhaust port 404.

[0017] Preferably, in the above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the upper cover body 401 serves as the main structure of the sliding upper cover, spanning the two reaction areas and the chamber air inlet connection part. When the upper cover completely covers the reaction area on one side for reaction, the gas flowing through the reaction area will be discharged through the upper cover exhaust port 404, and will not cause gas accumulation in the reaction area.

[0018] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the air inlet baffle 402 is located on both sides of the slidable upper cover 104, so that the air inlet baffle 402 can completely cover the arrayed air inlets, and concave grooves 405 are provided on both sides of the air inlet baffle 402. The entire slidable upper cover 104 has a total of four concave grooves, which are triangular in shape. The size is the same as the triangular cross-section of the long slide rail, forming a concave-convex fit therewith, ensuring the smooth sliding of the slidable upper cover 104 on the slide rail 305.

[0019] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the upper cover body 401 serves as the main structure of the slidable upper cover 104 and spans the chamber air inlet connection portion 101 and the two reaction areas.

[0020] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the air inlet baffle 402 is located on both sides of the upper cover, and the area enclosed by the lower edge and the left and right edges of the baffle can completely cover the arrayed small air inlet holes 302. Concave grooves 405 are also provided on both sides of the air inlet baffle. The entire slidable upper cover 104 has a total of four concave grooves, which are in the shape of a concave triangle and have the same size as the triangular cross-section of the long slide rail, forming a concave-convex fit therewith to ensure the smooth sliding of the slidable upper cover 104 on the slide rail.

[0021] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the upper cover handles 403 are located at two corners on the same side of the upper cover body and are connected to the small lead screw stepper motor 2 by a connecting rod 3.

[0022] Preferably, in the temperature and humidity control reaction chamber for inkjet printing enzymatic DNA synthesis, a small wireless temperature and humidity sensor is provided on the inner surface of the upper cover body 401 , and the small wireless temperature and humidity sensor is signal-connected to the humidity generator 5 .

[0023] The temperature and humidity control reaction chamber for inkjet printing enzymatic DNA synthesis is designed to further detect the temperature and humidity of the gas in the reaction chamber and facilitate timely feedback and adjustment. The probe of the small wireless temperature and humidity sensor is located inside the reaction area for detecting the temperature and humidity of the gas, monitoring the temperature and humidity of the gas in the reaction chamber in real time, and providing temperature and humidity measurement signals for temperature and humidity feedback control in the chamber.

[0024] Preferably, the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis is made of transparent acrylic material. In addition, other materials that can be used for production, such as resin, PEEK, PLA plastic, etc., can also be used.

[0025] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the chamber air inlet connection portion 101 and the first reaction area 102 / second reaction area 103 are completely sealed by clamping a sealing sheet in the middle and then screwing in a screw. In addition, any known gap sealing method including adhesives, pressure, tightening, etc. can also be used for complete sealing.

[0026] Preferably, the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis has threaded holes on the chamber air inlet connection portion 101, the first reaction area 102, the second reaction area 103 and the chip fixing cover 105, on which sealing sheets of corresponding sizes are provided. The overall assembly of the reaction chamber 1 is achieved by clamping the sealing sheets and tightening the screws.

[0027] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the sealing sheet is made of elastic material, and the elastic material includes but is not limited to PDMS, silicone, polysilane materials, polyurethane, acrylic amine, polyester imide, nitrile rubber, propylene rubber, etc.

[0028] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the cross-section of the slide rails on the upper cover supporting side walls 302 of the first reaction area 102 and the second reaction area 103 is triangular. In addition, any shape that can form a concave-convex fit and is stable, such as a semicircular arc, an elliptical arc, a rectangle, a trapezoid, and a zigzag shape, can also be used.

[0029] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the shapes of the arrayed air inlet holes on the hole array connecting wall 301 can be diversified, including but not limited to one of a circle, a triangle, a square, a rectangle, a pentagon, a hexagon, an octagon, other regular polygons, and a honeycomb.

[0030] Preferably, in the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, multiple groups of reaction chambers 1 are placed in series and parallel, so that the chamber has scalability and can synthesize more DNA synthesis chips at the same time.

[0031] The above-mentioned temperature and humidity control reaction chamber for inkjet printing enzymatic DNA synthesis requires the cooperation of other external devices during its working process, mainly including: a small screw stepper motor 2, a connecting rod 3, an inkjet printing device 4, a humidity generator 5 and a compressed gas cylinder 6; among them, the linear movement of the small screw stepper motor 2 drives the connecting rod 3 to realize the automatic movement of the sliding upper cover 104; the inkjet printing device 4 realizes the precise distribution of the four nucleotide monomers (A, T, C, G) for enzymatic DNA synthesis, as well as the liquid feeding and gas drying of the deprotection reagent and the cleaning reagent; the gas generated by the compressed gas cylinder 6 can be made to have a certain temperature and humidity after passing through the humidity generator 5. After the gas passes through the gas conduit 202 wrapped by the heating tape, the gas is stably input into the reaction chamber 1 by adjusting the cylinder pressure.

[0032] Technical Effects

[0033] The above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis includes four parts: a chamber air inlet connection, a reaction area, a slidable upper cover and a chip fixing cover. It integrates functions such as stable airflow input, chip fixing, and stable temperature and humidity maintenance. The chamber air inlet connection ensures that gas with a certain temperature and humidity is evenly introduced into the reaction area through multiple rows of parallel or cross-structured air inlets; the DNA synthesis chip is placed in the reaction area, and the chip fixing cover ensures stable clamping of the DNA synthesis chip; the slidable upper cover cooperates with the inkjet print head. When the inkjet print head is used to distribute the synthesis reagent to the surface of the DNA synthesis chip, the upper cover leaves the reaction area. When the reagent distribution is completed and the DNA enzymatic synthesis reaction is carried out, the upper cover covers the reaction area again, so that the synthesis reaction area maintains a relatively stable temperature and humidity gas environment. This reaction chamber can effectively control the temperature and humidity on the surface of the DNA synthesis chip to meet the reaction conditions for enzymatic DNA synthesis. It can also easily expand the reaction chamber through series and parallel connection of the same device, and simultaneously control the temperature and humidity environment of multiple chips. It has the advantages of simple operation, automation, and scalability. It solves the problems of easy evaporation of droplets, reaction temperature and humidity control, and waste liquid outflow during the inkjet printing enzymatic DNA synthesis reaction, ensuring the smooth progress of the enzymatic DNA synthesis process. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a diagram of the temperature and humidity controlled reaction chamber and its peripheral control system for inkjet printing enzymatic DNA synthesis according to the present invention.

[0035] Figure 2Schematic diagram of the temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis.

[0036] Figure 3 This is a structural diagram of the cabin air intake connection.

[0037] Figure 4 Schematic diagram of the structure of the cabin reaction area.

[0038] Figure 5 It is a structural diagram of the slidable upper cover.

[0039] Figure 6 Schematic diagram of the structure of the chip fixing cover.

[0040] Figure 7 Schematic diagram of the coordination of the chamber reaction area, DNA synthesis chip and fixed cover.

[0041] Figure 8 This is a schematic diagram of the air intake chamber and the air intake connecting plate in the cabin air intake connection.

[0042] Figure 9 Schematic diagram of the cabin reaction area.

[0043] Figure 10 Schematic diagram of the complex formed by the chamber reaction area, DNA synthesis chip and fixed cover plate, and the DNA synthesis chip and fixed cover plate.

[0044] Figure 11 Schematic diagram of the sliding cover.

[0045] Figure: 1-reaction chamber, 2-small screw stepper motor, 3-connecting rod, 4-inkjet printing device, 5-humidity generator, 6-compressed gas cylinder, 101-cabinet air inlet connection, 102-first reaction area, 103-second reaction area, 104-slidable upper cover, 105-chip fixing cover, 201-two-position three-way solenoid valve, 202-gas conduit, 203-air inlet chamber, 204-air inlet connecting plate, 205-partition plate, 301-hole array connection Wall, 302-upper cover supporting side wall, 303-chip placement platform, 304-bottom slope, 401-upper cover body, 402-air inlet baffle, 403-upper cover handle, 404-upper cover exhaust port, 405-concave groove, 501-rectangular cover, 502-rotatable clamping cover, A-length of the air inlet chamber, B-width of the air inlet chamber, C-height of the air inlet chamber, D-length of the air inlet connecting plate, E-width of the air inlet connecting plate, F-length of the upper cover supporting side wall, G- The width of the upper cover supporting side wall, H-the thickness of the connection position between the bottom slope and the hole array connecting wall, I-the thickness of the hole array connecting wall, K-the height of the slide rail, L-the width of the lower wall of the cabin, M-the width of the chip placement platform, N-the width of the boss, H1-the height of the boss, H2-the thickness of the DNA synthesis chip, H3-the thickness of the fixed cover, O-the length of the DNA synthesis chip, W1-the width of the DNA synthesis chip, P-the length of the fixed cover, W2-the width of the fixed cover, T-the width of the gap between the two DNA synthesis chips , Q-length of the sliding cover, R-length of the lower edge of the air inlet baffle, S-height of the left and right edges of the air inlet baffle, Y-thickness of the air inlet baffle, a-wall thickness of the air inlet chamber, d-diameter of the circular hole, e-diameter of the arrayed air inlet holes, f-center distance between holes in a horizontal row, g-center distance between holes in a vertical row, α-number of air inlet holes in a horizontal row, β-number of air inlet holes in a vertical row, p-center distance between adjacent threaded holes on the boss, u-length of the exhaust port of the cover, v-width of the exhaust port of the cover, w-distance from the exhaust port of the cover to the edge of the cover DETAILED DESCRIPTION

[0046] The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis of the present invention is described in detail below with reference to the examples and drawings. In the embodiments, the present invention can use a variety of materials to process the chamber, and the DNA synthesis chip used is a glass slide modified with primer chains.

[0047] Example 1

[0048] like Figure 1-11As shown, the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis includes a chamber air inlet connection part 101, a first reaction area 102, a second reaction area 103, a slidable upper cover 104 and a chip fixing cover 105, wherein the chamber air inlet connection part 101 is fixed between the first reaction area 102 and the second reaction area 103, the slidable upper cover 104 is placed above the chamber air inlet connection part 101, the first reaction area 102 and the second reaction area 103 and is slidably connected, and the chip fixing cover 105 is arranged in the first reaction area 102 and the second reaction area 103; wherein the main functions of each part are as follows: the chamber air inlet connection part 101 of the reaction chamber The gas connection part 101 can ensure that the air flow is evenly distributed into the first reaction area 102 and the second reaction area 103 respectively; the first reaction area 102 and the second reaction area 103 are used to place the DNA synthesis chip and perform enzymatic DNA synthesis reactions, and cooperate with the chip fixing cover 105 to ensure the stable clamping of the DNA synthesis chip; the slidable upper cover 104 can cooperate with the inkjet printing device 4. When the inkjet printing device 4 is used to distribute the synthesis reagent to the surface of the DNA synthesis chip, the upper cover leaves the reaction area. When the reagent distribution is completed and the DNA enzymatic synthesis reaction is performed, the upper cover re-covers the reaction area, so that the synthesis reaction area maintains a relatively stable temperature and humidity gas environment.

[0049] Preferably, the temperature and humidity control reaction chamber for inkjet printing enzymatic DNA synthesis, the chamber air inlet connection part 101 includes a two-position three-way solenoid valve 201, a gas conduit 202, an air inlet chamber 203 and an air inlet connection plate 204, a partition 205 is provided in the middle of the air inlet chamber 203, and the air inlet chamber 203 is divided into a left chamber and a right chamber by the partition 205, the air inlet chamber 203 is arranged between the first reaction area 102 and the second reaction area 103, the left chamber is connected to the first reaction area 102 through the air inlet connection plate 204 on one side thereof. The reaction area 102 is fixedly connected, and the right chamber is fixedly connected to the second reaction area 103 through an air inlet connecting plate 204 on one side thereof. The air inlet connecting plate 204 is symmetrically arranged on both sides of the air inlet chamber 203. The two-position three-way solenoid valve 201 includes an air inlet channel and two air outlet channels. The three channels are respectively connected to the humidity generator 5 and the left chamber and the right chamber of the air inlet chamber 203 through the gas duct 202. The two-position three-way solenoid valve is used to control the gas from the humidity generator 5 to be connected to only one side of the air inlet chamber 203 at the same time.

[0050] The main structure of the air inlet chamber 203 is a rectangular parallelepiped chamber structure. The outer length of the air inlet chamber 203 is A, the width is B, and the height is C. The thickness of each wall of the chamber is a. Two circular holes with a diameter of d are opened on the front wall of the chamber for connecting the gas conduit 202. The partition plate 205 set in the middle of the chamber is used to buffer the gas to ensure that the gases in the chambers on both sides are not connected to each other, while being connected to their respective synthesis reaction areas; the air inlet connecting plate is a rectangular plate with a length of D, a width of E, and a thickness of b. A rectangular through hole with a diameter of B and a width of C, the size of which matches the external size of the air inlet chamber, can be sealed and fixed to the peripheral parts on both sides of the air inlet chamber by gluing to complete the assembly of the main body of the cabin air inlet connection part; threaded holes are provided on the periphery of the air inlet connection plate, which are symmetrical in the upper and lower parts and left and right parts. By clamping a gasket between the air inlet connection plate 204 and the first reaction area 102 / the second reaction area 103 and tightening them with screws, a sealed connection between the cabin air inlet connection part and the reaction area is achieved to ensure air tightness. The air intake connecting plate 204 is a separate rectangular structure, which is installed on both sides of the air intake chamber 203. The air intake connecting plate 204 is a rectangular plate with a length of D, a width of E, and a thickness of b. The size of the rectangular through hole hollowed out in the middle is the same as the size of the air intake chamber 203. Its cross-section length is B and width is C, which can facilitate the air intake connecting plate 204 to be accurately inserted into both sides of the air intake chamber 203, and can be further fixed and sealed by glue sealing. Threaded holes are also provided on the air intake connecting plate 204, which is assembled by clamping a gasket between the air intake connecting plate 204 and the first reaction area 102 / second reaction area 103 and tightening it with screws.

[0051] The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis is characterized in that the first reaction area 102 / second reaction area 103 is a dustpan-shaped structure, including a hole array connecting wall 301, an upper cover supporting side wall 302, and a chamber lower wall composed of a chip placement platform 303 and a bottom slope 304. The hole array connecting wall 301 is located at one end of the bottom slope 304 of the chamber lower wall, and the upper cover supporting side wall 302 is symmetrically arranged on both sides of the bottom slope 304 of the chamber lower wall and fixedly connected to both sides of the hole array connecting wall 301. The bottom slope 304 is adjacent to the upper cover supporting side wall 302, and a chip placement platform 303 for supporting and fixing the DNA synthesis chip is provided. The thickness of the bottom slope 304 at the connection position with the hole array connecting wall 301 gradually decreases in the direction away from the hole array connecting wall 301, so that a downward angle is formed between the bottom slope 304 and the horizontal plane. Specifically,

[0052] The hole array connecting wall 301 is located at one end of the dustpan-shaped structure, and has a length of D, a width of E, and a thickness of I. The middle portion of the connecting wall has arrayed air inlet holes arranged in a parallel or cross pattern. The diameter of each air inlet hole is the same, and the hole center distances between adjacent air inlet holes in each horizontal row and each vertical row are equal to ensure that the air flow flows evenly and stably into the reaction area. The diameter of each air inlet hole is e, each horizontal row has α air inlet holes, and the hole center distances between the horizontal rows are f, and each vertical row has β air inlet holes, and the hole center distances between the vertical rows are g. When a gasket is clamped between the air inlet connecting plate 204 and the hole array connecting wall 301 and tightened with screws, the area covered by the air inlet hole array corresponds to the ventilation area inside the air inlet chamber 203, that is, the following conditions are satisfied:

[0053] f*α+e>B-2a>f*(α-1)+e

[0054] g*β+e>C-2a>g*(β-1)+e

[0055] This can ensure effective ventilation of the arrayed air inlet holes, and the gas will all enter the first reaction area 102 / the second reaction area 103 after coming out of the air inlet chamber 203 without accumulation. The upper cover supporting side wall 302 is F in length, G in width, and E in height. A long strip slide rail is provided above the side wall, and its cross section is an isosceles triangle with a base length of G, a height of K, and a length of F. Its function is to cooperate with the triangular recess of the slidable upper cover 104 to support the upper cover, and to ensure smooth sliding of the upper cover between the first reaction area 102 and the second reaction area 103. The lower wall of the chamber is composed of a chip placement platform 303 and a bottom inclined surface 304. The length of the lower wall of the chamber is F and the width is L. The width of the lower wall of the chamber is equal to the length of the hole array connecting wall 301 minus the thickness of the upper cover supporting side walls 302 on both sides, that is:

[0056] L=D-2G

[0057] On the lower wall of the chamber, adjacent to the upper cover supporting side wall, a chip placement platform 303 with a length of F and a width of M is set for supporting and fixing the DNA synthesis chip; on the two chip placement platforms 303, adjacent to the upper cover supporting side wall, a boss with a length of F, a width of N, and a height of H1 is set, and threaded holes with a hole center distance of p are set on the long bosses to cooperate with the chip fixing cover 105 to achieve fixed clamping of the DNA synthesis chip, so that the DNA synthesis chip can be kept in place during chamber movement, reagent injection and gas purging. Stable; between the chip placement platforms 303 on both sides is a bottom slope 304 with a length of F and a width of L-2M. The thickness of the bottom slope 304 at the connection position with the hole array connecting wall 301 is H, and the thickness of the bottom slope 304 gradually decreases in the direction away from the hole array connecting wall 301, so that a downward angle is formed between the bottom slope 304 and the horizontal plane, ensuring that the waste liquid generated by the DNA synthesis reaction can flow smoothly along the bottom slope 304 into the waste liquid tank set below the cabin to achieve waste liquid collection. A hydrophobic coating can be applied to the bottom slope 304 to increase the hydrophobicity of the slope, improve the waste liquid collection efficiency, and reduce the risk of waste liquid accumulation. The structural dimensions of the lower wall of the cabin are determined according to the length O, width W1, and thickness H2 of the DNA synthesis chip. The number of DNA synthesis chips placed side by side in the chamber is X (X is greater than or equal to 1). In order to clean the waste liquid generated by the DNA synthesis reaction from the chip surface, a gap of width T is set between the chips. At the same time, in order to prevent the upper cover exhaust port 404 from interfering with the temperature and humidity of the synthesis chip surface, the DNA synthesis chips are placed only in the area between the hole array connecting wall 301 and the upper cover exhaust port 404. Therefore, the following conditions are met:

[0058] Fvw>(W1+T)*X

[0059] The chip placement platform 303 is used to support and fix the DNA synthesis chip. Therefore, its dimensions must meet the following requirements:

[0060] L-2N≥O>L-2M

[0061] H1=H2

[0062] In addition, the thickness H at the connection between the chamber bottom slope 304 and the hole array connecting wall 301 must ensure that after the DNA synthesis chip is placed, it does not exceed the height from the lower edge of the arrayed air inlet to the chamber bottom, that is:

[0063] H+H2+H3<(EC) / 2+a

[0064] Otherwise, it will affect the temperature and humidity of the gas entering the synthesis reaction area.

[0065] The temperature and humidity controlled reaction chamber for inkjet-printed enzymatic DNA synthesis described above includes two types of chip-fixing cover plates 105: a rectangular cover plate 501 and a rotatable clamping cover plate 502. The chip-fixing cover plate 105 is a rectangular cover plate 501 with a length W2, a width P, and a thickness H3. One side of the cover plate has threaded holes with a center-to-center distance p, which mate with the threaded holes on the chip placement platform 303. To ensure that the cover plate can firmly press the DNA synthesis chip, its length is equal to the chip width, that is:

[0066] W1=W2

[0067] Its width is greater than the boss width, that is:

[0068] P>N

[0069] The advantages of this rectangular cover sheet are that it has a large contact area with the DNA synthesis chip, can achieve stable clamping, and can adjust the length of the cover sheet according to the number of chips to achieve single-chip coverage, or use a longer cover sheet to achieve simultaneous coverage of multiple chips.

[0070] The chip fixing cover 105 is a rotatable clamping cover 502, which consists of a flat plate structure and an arc-shaped structure. The flat plate structure is semicircular, ensuring that the cover does not touch the upper cover support side wall 302 during rotation. A single threaded hole is provided on the rotatable clamping cover for fixing it to the boss of the chip placement platform 303. The rotatable clamping cover has the advantage of facilitating the installation of the DNA synthesis chip without having to disassemble it each time.

[0071] The temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, wherein the slidable upper cover structure 104 includes an upper cover body 401, an air inlet baffle 402, and an upper cover handle 403. The upper cover body 401, as the main structure of the slidable upper cover, spans the two reaction areas and the chamber air inlet connection portion. Its total length Q is equal to the total length of the air inlet chamber 203, the first reaction area 102 / the second reaction area 103 on one side, and the thickness of the air inlet baffle 402, that is, it satisfies:

[0072] Q=F+2I+A+Y

[0073] At the same time, a cover exhaust port 404 with a length of u and a width of v is provided at a distance w from both ends of the upper cover body 401 to its boundary. When the upper cover completely covers the reaction area on one side for reaction, the gas flowing through the reaction area will be discharged through the cover exhaust port 404, and will not cause gas accumulation in the reaction area. The air inlet baffle 402 is located on both sides of the slidable upper cover 104. The lower edge length of the air inlet baffle is R, the left and right edge heights are S, and the baffle thickness is Y, satisfying:

[0074] D-2G>R>f*(α-1)+e

[0075] EKH-H2-H3>S>g*(β-1)+e

[0076] The purpose is to enable the air inlet baffle 402 to completely cover the arrayed air inlets, protecting the arrayed air inlets from contamination caused by splashing liquid droplets during the cleaning and drying process of the DNA synthesis chip; at the same time, it is necessary to avoid bumping the chip fixing cover on the chip placement platform 303 during the sliding process of the upper cover. It is worth noting that above the reaction area, away from the arrayed air inlets, is the upper cover exhaust port 404. To prevent the upper cover exhaust port 404 from interfering with the temperature and humidity of the synthesis chip surface, the DNA synthesis chip is not placed in this area. Concave grooves 405 are provided on both sides of the air inlet baffle 402. The entire slidable upper cover 104 has a total of four concave grooves, which are triangular in shape. Their dimensions are the same as the triangular cross-section of the long slide rail, forming a concave-convex fit with it, ensuring the smooth sliding of the slidable upper cover 104 on the slide rail 305. The upper cover body 401 serves as the main structure of the sliding upper cover 104, spanning the chamber air inlet connection part 101 and the two reaction areas (the first reaction area 102 and the second reaction area 103). Its total length Q is equal to the sum of the thicknesses of the air inlet chamber 203, the reaction area on one side (the first reaction area 102 or the second reaction area 103) and the air inlet baffle 402. At the same time, upper cover exhaust ports 404 with a length of u and a width of v are respectively provided at a distance w from both ends of the upper cover body 401 to its boundary. When the upper cover completely covers the reaction area on one side (the first reaction area 102 or the second reaction area 103) for reaction, the upper cover exhaust port 404 is located above the tail of the reaction area, and the gas flowing through the reaction area will be discharged through the upper cover exhaust port 404, and will not cause gas accumulation in the reaction area. The air inlet baffle 402 is located on both sides of the upper cover. The area enclosed by the lower edge and left and right edges of the baffle can completely cover the arrayed small air inlet holes 302. The lower edge of the baffle is R long and the left and right edges are S high. During the cleaning and drying process of the DNA synthesis chip, the air inlet holes are protected from contamination caused by splashing droplets. Concave grooves 405 are also provided on both sides of the air inlet baffle. The entire slidable upper cover 104 has a total of four concave grooves, which are in the shape of a concave triangle. Their size is the same as the triangular cross-section of the long slide rail, and can form a concave and convex fit with it to ensure the smooth sliding of the slidable upper cover 104 on the slide rail. The upper cover handle 403 is located at two corners on the same side of the upper cover body and is connected to the small lead screw stepper motor 2 by a connecting rod 3. One end of the connecting rod 3 is buckled on the raised handle 401, and the other end is buckled on the small lead screw stepper motor 2. The lead screw stepper motor drives the connecting rod 3 to move linearly on the lead screw to realize the automatic sliding of the upper cover. At the same time, the raised handle on one side of the upper cover can ensure that the inkjet printing droplets are not affected when being distributed onto the DNA synthesis chip.

[0077] The above-mentioned temperature and humidity control reaction chamber for inkjet printing enzymatic DNA synthesis is provided with a small wireless temperature and humidity sensor on the inner surface of the upper cover body 401 to monitor the temperature and humidity of the gas in the reaction chamber in real time, and provide temperature and humidity measurement signals for the temperature and humidity feedback control in the chamber. The small wireless temperature and humidity sensor is connected to the humidity generator 5 signal to further detect the temperature and humidity of the gas in the reaction chamber and facilitate timely feedback and adjustment. The probe of the small wireless temperature and humidity sensor is located inside the reaction area for detecting the temperature and humidity of the gas, monitoring the temperature and humidity of the gas in the reaction chamber in real time, and providing temperature and humidity measurement signals for the temperature and humidity feedback control in the chamber.

[0078] The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis is constructed from transparent acrylic (other materials, such as resin, PEEK, or PLA, can also be used). The chamber air inlet connection 101 and the first reaction area 102 / second reaction area 103 are completely sealed by clamping a sealing sheet and then screwing in screws. Alternatively, any other known gap sealing method, including adhesives, pressure, or tightening, can be used. The chamber air inlet connection 101, the first reaction area 102, the second reaction area 103, and the chip fixing cover 105 all contain threaded holes with correspondingly sized sealing sheets installed. Assembly of the reaction chamber 1 is achieved by clamping the sealing sheet and then tightening the screws. The sealing sheet is made of an elastic material, including but not limited to PDMS, silicone, polysilane, polyurethane, acrylic amine, polyesterimide, nitrile rubber, and propylene rubber.

[0079] In the above-mentioned temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis, the cross-section of the slide rails on the upper cover supporting side walls 302 on both sides of the first reaction area 102 and the second reaction area 103 is triangular (it can also be any shape that can form a concave-convex fit and is stable, such as a semicircular arc, an elliptical arc, a rectangle, a trapezoid, a zigzag, etc.); the shape of the arrayed air inlet holes on the hole array connecting wall 301 can be diversified, including but not limited to one of a circle, a triangle, a square, a rectangle, a pentagon, a hexagon, an octagon, other regular polygons, and a honeycomb.

[0080] The temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis described above can be arranged in series and parallel, making the chamber scalable and capable of simultaneously synthesizing more DNA synthesis chips. The reaction chamber requires the cooperation of other external devices, mainly including: a small screw-type stepper motor 2, a connecting rod 3, an inkjet printer 4, a humidity generator 5, and a compressed gas cylinder 6. The linear movement of the small screw-type stepper motor 2 drives the connecting rod 3 to achieve automated movement of the slidable upper cover 104. The inkjet printer 4 enables precise distribution of the four nucleotide monomers (A, T, C, and G) used in enzymatic DNA synthesis, as well as the delivery of deprotection and cleaning reagents and gas drying. The gas generated by the compressed gas cylinder 6 passes through the humidity generator 5, which gives the gas a certain temperature and humidity. After the gas passes through the gas conduit 202 wrapped in the heating tape, the gas cylinder pressure is adjusted to ensure stable input into the reaction chamber 1.

[0081] The dimensions of the various components in the temperature and humidity control reaction chamber for inkjet-printed enzymatic DNA synthesis are exemplified as follows:

[0082] The size of the DNA synthesis chip used was 75 mm × 25 mm × 1 mm (length × width × height).

[0083] The diameter of the gas channel of the two-position three-way solenoid valve 201 of the cabin air inlet connection is at least 4 mm or wider, the inner diameter of the gas conduit 202 is at least 3 mm or wider, and the outer diameter of the gas conduit 202 is at least 4 mm or wider.

[0084] The air inlet chamber 203 is 55 mm long, 90 mm wide, and 16 mm high. Each wall is 2 mm thick, and the circular hole in the front wall is 8 mm in diameter. The outer edge of the air inlet connecting plate 204 is 120 mm long and 30 mm wide, with a rectangular through-hole in the center measuring 90 mm long and 16 mm wide.

[0085] The hole array connecting wall 301 is 120 mm long, 30 mm high, and 10 mm thick. Each hole in the arrayed air inlet hole has a diameter of 1.5 mm. Each horizontal row has 27 holes with a 3 mm center-to-center spacing. Each vertical row has 6 holes with a 2 mm center-to-center spacing.

[0086] The upper cover supporting side wall 302 is 180 mm long, 10 mm wide and 30 mm high. The length of the slide rail is 180 mm. The bottom length of the triangle in the cross section of the slide rail is 10 mm and the height is 5 mm.

[0087] The chip placement platform 303 is 180 mm long, 17.5 mm wide, and 10 mm high. The bosses are 180 mm long, 12.5 mm wide, and have a protrusion height of 1 mm. The distance between the two bosses is 75 mm. The bottom slope 304 is 180 mm long and 65 mm wide, and is 9 mm thick at the connection point with the hole array connection wall 301.

[0088] The upper cover body 401 has a total length of 260mm, the upper cover exhaust port 404 is 20mm long, 100mm wide, and 5mm away from the sides of the upper cover. The lower edge of the air inlet baffle 402 is 90mm long, the left and right edges are 13mm high, and the thickness is 1mm.

[0089] The number of threaded holes on the upper side and the number of threaded holes on the lower side of the air intake connection plate 204 are equal and symmetrical, namely, 5 or more, and the number of threaded holes on the left side and the number of threaded holes on the right side are equal and symmetrical, namely, 3 or more.

[0090] The number of upper and lower threaded holes on the outer periphery of the hole array connecting wall 301 is the same, symmetrical, and there are 5 or more of them, and their positions are the same as those of the threaded holes on the air intake connecting plate 204. The number of left and right threaded holes is the same, symmetrical, and there are 3 or more of them, and their positions are the same as those of the threaded holes on the air intake connecting plate 204.

[0091] The diameter of the arrayed air inlet holes on the hole array connecting wall 301 is at least 0.5 mm, 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm or wider.

[0092] The number of the arrayed air inlet holes on the hole array connecting wall 301 in the transverse direction is at least 10, 15, 20, 25 or more, and the number in the longitudinal direction is at least 5, 6, 7, 8, 9, 10 or more.

[0093] The number of synthesis chips that can be carried by the chamber reaction area 102 / 103 is 1, 2, 3, 4, 5 or more.

[0094] The relevant threaded hole diameter used is at least 1mm, 2mm, 3mm, 4mm, 5mm or wider.

[0095] The relevant screw model used matches the threaded hole, and its specification is at least one of M1, M2, M3, M4, M5, etc.

[0096] The overall working process of the temperature and humidity controlled reaction chamber for inkjet printing enzymatic DNA synthesis is as follows:

[0097] (1) The initial position of the reaction chamber 1 is: the slidable upper cover 104 is completely located above the first reaction area 102, and the DNA synthesis chip is fixed to the first reaction area 102 / second reaction area 103 via the chip fixing cover 105. The number of DNA synthesis chips can be determined according to the requirements of the synthesis;

[0098] (2) Printing a synthesis solution containing deoxyribonucleotides (dNTPs) and terminal deoxynucleotidyl transferase (TdT enzyme) on the DNA synthesis chip fixed on the second reaction area 103 by the inkjet printing device 4;

[0099] (3) After the inkjet printing is completed, the slidable upper cover 104 slides to the top of the second reaction area 103 under the action of the small screw stepper motor 2. At this time, the two-position three-way solenoid valve 201 on the side leading to the second reaction area 103 is opened, and the gas after passing through the humidity generator 5 is directed to the second reaction area 103. The second reaction area 103 is filled with gas with a certain temperature and humidity to ensure efficient enzymatic DNA synthesis;

[0100] (4) After the enzymatic DNA synthesis reaction is completed, the slidable upper cover 104 slides to the top of the first reaction area 102 under the action of the small screw stepper motor 2, and then the DNA synthesis chip is subjected to processes such as deprotection reagent liquid addition, air drying, cleaning reagent liquid addition, and air drying by the inkjet printing device 4. The waste liquid will flow into the waste liquid tank through the bottom inclined surface 304, completing one enzymatic DNA synthesis cycle;

[0101] (5) During the process of enzymatic DNA synthesis reaction in the second reaction area 103, the inkjet printing device 4 can be moved to the first reaction area 102 to distribute the synthesis liquid. By repeating the above steps (1), (2), (3), and (4), a cycle of alternating enzymatic DNA synthesis in the two reaction areas (first reaction area 102 / second reaction area 103) is achieved.

[0102] In summary, the temperature and humidity control reaction chamber device for inkjet printing enzymatic DNA synthesis of the present invention has the following characteristics:

[0103] ① By designing two reaction areas (first reaction area 102 / second reaction area 103), it is ensured that the two areas can alternately carry out enzymatic DNA synthesis cycles simultaneously, effectively improving the time utilization efficiency and synthesizing more DNA in the same time;

[0104] ② The slidable upper cover 104 is designed to cooperate with the inkjet printing device 4. When inkjet printing is performed, the slidable upper cover 104 is driven by the small lead screw stepper motor 2 to leave the reaction area (first reaction area 102 / second reaction area 103). When enzymatic DNA synthesis is performed, it returns to the reaction area to control the temperature and humidity environment of the synthesis area.

[0105] ③ The compressed gas cylinder 6 generates a stable pressure airflow to achieve stable gas input. After passing through the cabin air inlet connection 101 and the reaction area (first reaction area 102 / second reaction area 103), the gas can be evenly dispersed. Both ensure that the temperature and humidity of the gas can be kept uniform and consistent when entering the reaction area;

[0106] ④ By designing the chip placement platform 303 of the reaction area (first reaction area 102 / second reaction area 103), and matching it with the chip fixing cover 105, the DNA synthesis chip can be stably clamped and the chip can be easily disassembled and installed;

[0107] ⑤ By designing an air inlet baffle 402 that can completely cover the arrayed air inlet holes 302, it prevents droplets from splashing and contaminating the air inlet holes during the cleaning and drying process;

[0108] ⑥ By designing the inclined bottom slope 304 of the reaction area (first reaction area 102 / second reaction area 103), the waste liquid purged during the inkjet printing process can flow smoothly into the waste liquid tank through the gap and slope between the two chips, thereby facilitating the collection of the waste liquid.

[0109] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis, characterized by: It includes a chamber air inlet connection part, a first reaction area, a second reaction area, a slidable upper cover and a chip fixing cover. The chamber air inlet connection part is fixed between the first reaction area and the second reaction area. The slidable upper cover is placed above the chamber air inlet connection part, the first reaction area and the second reaction area and is slidably connected. The chip fixing cover is arranged in the first reaction area and the second reaction area.

2. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 1, characterized in that: The cabin air inlet connection part includes a two-position three-way solenoid valve, a gas duct, an air inlet chamber and an air inlet connecting plate. A partition is provided in the middle of the air inlet chamber, and the air inlet chamber is divided into a left chamber and a right chamber by the partition. The air inlet chamber is arranged between the first reaction area and the second reaction area. The left chamber is fixedly connected to the first reaction area through the air inlet connecting plate on one side thereof, and the right chamber is fixedly connected to the second reaction area through the air inlet connecting plate on one side thereof. The air inlet connecting plates are symmetrically arranged on both sides of the air inlet chamber. The two-position three-way solenoid valve includes an air inlet channel and two air outlet channels. The three channels are respectively connected to the humidity generator and the left chamber and the right chamber of the air inlet chamber through the gas duct. The two-position three-way solenoid valve is used to control the gas from the humidity generator to be connected to the air inlet chamber on one side at the same time.

3. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 2, characterized in that: The main structure of the air inlet chamber is a rectangular chamber structure, with two circular holes for connecting the gas duct on the front wall of the chamber, and a partition for buffering the gas is provided in the middle of the chamber; the air inlet connecting plate is a rectangular plate with a rectangular through hole in the middle, and the size of the through hole matches the external size of the air inlet chamber; the air inlet connecting plate and the first reaction area / the second reaction area are sealed and connected by a clamping gasket.

4. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 2, characterized in that: The air intake connecting plate is a separate rectangular structure, which is installed on both sides of the air intake chamber. The size of the rectangular through hole hollowed out in the middle of the air intake connecting plate is the same as the size of the air intake chamber, which facilitates the air intake connecting plate to be accurately inserted into both sides of the air intake chamber and sealed and fixed. The air intake connecting plate and the first reaction area / second reaction area are tightly assembled through a clamping gasket.

5. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 1, characterized in that: The first reaction area / second reaction area is a dustpan-shaped structure, including a hole array connecting wall, an upper cover supporting side wall, and a lower wall of the cabin composed of a chip placement platform and a bottom slope. The hole array connecting wall is respectively located at one end of the bottom slope of the lower wall of the cabin, and the upper cover supporting side walls are symmetrically arranged on both sides of the bottom slope of the lower wall of the cabin and fixedly connected to the two sides of the hole array connecting wall. The bottom slope is adjacent to the upper cover supporting side wall, and a chip placement platform for supporting and fixing the DNA synthesis chip is provided. The thickness of the bottom slope at the connection position with the hole array connecting wall gradually decreases in the direction away from the hole array connecting wall, so that a downward angle is formed between the bottom slope and the horizontal plane.

6. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 5, characterized in that: The hole array connecting wall is located at one end of the dustpan-shaped structure, and the middle part of the hole array connecting wall has arrayed air inlet holes arranged in a parallel or cross pattern. The diameter of each air inlet hole is the same, and the hole center distances between adjacent air inlet holes in each horizontal row and each vertical row are equal.

7. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 5, characterized in that: The lower wall of the chamber is composed of a chip placement platform and a bottom inclined surface. The width of the lower wall of the chamber is equal to the length of the hole array connecting wall minus the thickness of the upper cover support side walls on both sides. A chip placement platform is set on the lower wall of the chamber adjacent to the upper cover support side walls for supporting and fixing the DNA synthesis chip; a long boss is set on each of the two chip placement platforms adjacent to the upper cover support side walls, which cooperates with the chip fixing cover to achieve fixed clamping of the DNA synthesis chip, so that the DNA synthesis chip remains stable during chamber movement, reagent injection and gas purging; between the chip placement platforms on both sides is the bottom inclined surface, and a downward angle is formed between the bottom inclined surface and the horizontal plane to ensure that the waste liquid generated by the DNA synthesis reaction can flow smoothly along the bottom inclined surface into the waste liquid tank set below the chamber to realize the collection of the waste liquid. The bottom inclined surface is coated with a hydrophobic coating to increase the hydrophobicity of the inclined surface, improve the collection efficiency of the waste liquid, and reduce the risk of waste liquid accumulation.

8. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 1, characterized in that: The chip fixing cover plate includes two types: a rectangular cover plate and a rotatable clamping cover plate.

9. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 1, characterized in that: The slidable upper cover structure includes an upper cover body, an air inlet baffle and an upper cover handle. The air inlet baffle is fixed on both sides of the upper cover body. The upper cover handle is located on the upper surface of the upper cover body. The upper cover body is provided with an upper cover exhaust port.

10. The temperature and humidity controlled reaction chamber for inkjet enzymatic DNA synthesis according to claim 9, characterized in that: A small wireless temperature and humidity sensor is provided on the inner surface of the upper cover body, and the small wireless temperature and humidity sensor is connected to the humidity generator signal.

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