Peripheral blood specificity-based early-stage lung cancer diagnosis auxiliary equipment

Abstract

The invention relates to the technical field of medical equipment, in particular to peripheral blood specificity-based early-stage lung cancer diagnosis auxiliary equipment, which comprises a shell, a centrifugal mechanism for performing centrifugal separation on peripheral blood is arranged in the shell, the centrifugal mechanism comprises a placement rack rotationally matched with the shell, and a test tube for accommodating the peripheral blood is placed on the placement rack; turbidity detection mechanisms for detecting layering heights of peripheral blood inside and outside the test tubes after centrifugation are arranged on two sides of the placement rack; the control panel is electrically connected with the centrifugal mechanism and the turbidity detection mechanism; the control panel is electrically connected with a transfer mechanism for sucking peripheral blood inside and outside the test tube; the transfer mechanism comprises a center block, one end of the center block close to the test tube is communicated with a suction tube, and one end of the center block far away from the suction tube is communicated with a magnetic separation mechanism for magnetic field screening of magnetic beads; the control panel is used for controlling the transfer mechanism to work based on the layering height at the current moment; the capture efficiency and purity of the magnetic beads on the markers are guaranteed, and the diagnosis accuracy is further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to an auxiliary device for diagnosing early lung cancer based on peripheral blood specificity. Background Art

[0002] Currently, early diagnosis of lung cancer mainly relies on imaging examinations, molecular biology and tumor marker detection, and cytology and pathology examinations.

[0003] Among them, imaging examinations rely on CT images to screen for lung cancer. They are easy to operate but have low sensitivity and are prone to missing small nodules. They are mostly used for preliminary screening. Moreover, CT and PCR instruments are bulky and require external stable power supplies and cooling systems, making it difficult to meet the needs of bedside testing or mobile screening.

[0004] Cytological and pathological examinations, such as sputum cytology and bronchoscopy, require professional personnel to perform. Although samples can be taken directly from the lesion, the process is complicated and difficult to popularize in primary medical institutions.

[0005] Molecular biology and tumor marker testing detects blood markers or DNA, which has high sensitivity but limited specificity for a single marker and is easily interfered with by other diseases. Peripheral blood contains a variety of lung cancer-specific markers, such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA). These can be captured and separated by magnetic beads to specifically bind to different markers, reducing nonspecific binding and background interference, providing a convenient, safe and efficient solution for the early diagnosis of lung cancer.

[0006] However, the existing peripheral blood is usually stored in test tubes during the detection process, and then purified by specific binding with magnetic beads after centrifugation; in the process of separating blood samples by magnetic bead method, the lung cancer specific marker is bound by the specific antibody modified on the surface of the magnetic beads, and then the magnetic beads are adsorbed by the magnetic field to achieve the separation of the lung cancer specific marker from the peripheral blood. Due to factors such as weak magnetic response of the magnetic beads, insufficient magnetic field of the equipment, improper setting of operating parameters (such as too short magnetic absorption time), the target capture efficiency and purity are reduced, thereby reducing the diagnostic accuracy. Therefore, the present invention provides a peripheral blood-specific early lung cancer diagnosis auxiliary device for ensuring the capture efficiency and purity of the magnetic beads for the marker, thereby improving the diagnostic accuracy. Summary of the Invention

[0007] To solve the above problems, the present invention provides an auxiliary diagnostic device for early lung cancer based on peripheral blood specificity, which ensures the capture efficiency and purity of magnetic beads for markers, thereby improving diagnostic accuracy.

[0008] To achieve the above objectives, the present invention provides a peripheral blood-specific early lung cancer diagnostic aid, comprising a housing, a centrifuge mechanism disposed within the housing for centrifuging peripheral blood, the centrifuge mechanism comprising a rack rotatably engaged with the housing, on which test tubes for containing peripheral blood are placed; and turbidity detection mechanisms disposed on both sides of the rack for detecting the height of the layers of peripheral blood in the test tubes after centrifugation.

[0009] The invention also includes a control panel electrically connected to the centrifugal mechanism and the turbidity detection mechanism, and the control panel is electrically connected to a transfer mechanism for aspirating peripheral blood in and out of the test tube;

[0010] The transfer mechanism includes a central block, one end of the central block close to the test tube is connected to a suction tube, and the other end of the central block away from the suction tube is connected to a magnetic separation mechanism for screening magnetic beads by magnetic field. The central block is provided with a plurality of connecting pipes connected to the suction tube and the magnetic separation mechanism, and the connecting pipes are connected to a plurality of injection pipes for transporting magnetic beads with different surface modification layers. The injection pipes are connected to electromagnetic valves.

[0011] The control panel is used to control the solenoid valve to work based on the layer height at the current moment.

[0012] Furthermore, the turbidity detection mechanism includes a clamping block, which is symmetrically arranged with the placement frame as the center, the clamping block and the placement frame are slidably matched, one end of the clamping block is slidably matched with a screw rod, the screw rod is rotatably matched with the placement frame, and one end of the screw rod is coaxially fixedly connected to a driving member;

[0013] A mounting hole for placing the test tube is opened at the center of the placement rack. A light-emitting unit for emitting infrared light and a detection unit for detecting scattered light are provided on both sides of the mounting hole. The detection unit is electrically connected to the control panel.

[0014] The control panel compares the consistency of the scattered light intensity before and after the current time. If they are consistent, the duration is recorded and the layer height is marked based on the duration and the power of the driving component. If they are inconsistent, a layer mark is established and the duration is reset to zero.

[0015] Furthermore, the central block is located above the test tube, and a first electric push rod with adjustable extension length is fixedly connected to the top of the central block, the first electric push rod is electrically connected to the control panel, and an end of the first electric push rod away from the test tube is fixedly connected to a bracket, and the bracket is fixedly connected to the top of the housing;

[0016] The suction pipe is located on the side of the central block away from the first electric push rod, the suction pipe is arranged circumferentially with the central block as the center, and a connection port is opened on the side of the suction pipe away from the central block; a transfer block is rotatably matched in the central block, the butt joint pipe is located in the transfer block, the butt joint pipe is arranged circumferentially with the transfer block as the center, and the butt joint pipe is located between the suction pipe and the magnetic suction mechanism;

[0017] A second motor is coaxially fixedly connected to the top of the transfer block, and the second motor is fixedly connected to the central block; a plurality of injection cavities for storing magnetic beads with different surface modification layers are opened in the transfer block, and the injection tube is located between the injection cavity and the docking tube;

[0018] When the butt joint tube is aligned with the suction tube, the suction tube is communicated with the magnetic separation mechanism through the butt joint tube.

[0019] Furthermore, the magnetic separation mechanism includes a plurality of glass plates, which are arranged in a ring shape with the first electric push rod as the center, and the side of the glass plate away from the first electric push rod is fixedly connected to the housing;

[0020] There is a gap between adjacent glass plates, and the gap is connected to a drainage tube; the transfer mechanism also includes a negative pressure tube connected to the drainage tube, and the end of the negative pressure tube away from the drainage tube is connected to a negative pressure machine for generating negative pressure suction;

[0021] The drainage tube is fixedly connected to the fixed end of the first electric push rod, the end of the drainage tube away from the glass plate is wound around the output end of the first electric push rod, and the drainage tube is connected to the suction tube through the docking tube;

[0022] Electromagnets for adjusting the magnetic field strength are provided on both sides of the glass plate. The electromagnets are electrically connected to the control panel. The electromagnets are located on one side of the glass plate. Several water pipes are connected to the end of the gap away from the drainage pipe. The water pipes are located on both sides of the bottom of the glass plate and are connected to recovery tanks respectively.

[0023] Furthermore, a sealing mechanism for adjusting the size of the opening of the connection port is provided on one side of the connection port. The sealing mechanism includes a slider that slides with the central block. The sliders are symmetrically arranged with the suction pipe as the center.

[0024] A pull rod is provided between the slider and one side of the suction pipe, one end of the pull rod is hinged to the slider, and the other end of the pull rod is hinged to the suction pipe; a second electric push rod is fixedly connected to the side of the slider away from the suction pipe, the second electric push rod is slidably matched with the center block, and the second electric push rod is electrically connected to the control panel.

[0025] Furthermore, the transfer block is a transparent glass block, and an ultraviolet lamp is fixedly connected to one side of the center block close to the transfer block. The ultraviolet lamp is electrically connected to the control panel, and the ultraviolet lamp is located between adjacent suction tubes; and several shielding layers are fixedly connected to the transfer block, and the shielding layers are located between adjacent docking tubes.

[0026] Furthermore, an end of the glass plate close to the first electric push rod is higher than an end of the glass plate away from the first electric push rod, and the negative pressure tube is located at the highest point of the drainage tube.

[0027] Furthermore, the side of the glass plate close to the electromagnet is higher than the side of the glass plate far from the electromagnet.

[0028] Furthermore, the control panel is also used to record and store the position of each suction tube and the type of magnetic beads corresponding to the solenoid valve at each position, mark the second electric push rod based on the position of each suction tube, and add a basic opening size addition instruction based on the layer marks corresponding to different layer heights. The basic opening size addition instruction is used to mark the size of the basic opening in a clockwise direction.

[0029] The control panel then compares the extended length of the first electric push rod with the layer height at the current time, obtains the layer mark that the extended length is consistent with the layer height, and sends a start instruction to the corresponding second electric push rod based on the layer mark; at the same time, it obtains the corresponding magnetic bead type based on the layer mark, and sends a start instruction to the corresponding solenoid valve based on the magnetic bead type.

[0030] Furthermore, a support shaft is rotatably provided at the center of the transfer block, the support shaft is located at one end of the transfer block close to the suction pipe, and the support shaft is fixedly connected to the center block;

[0031] A plurality of photosensors are fixedly connected to the support shaft, and the photosensors are located at the angle between adjacent suction pipes; and when the butt-joint pipe is docked with the suction pipe and the drainage pipe, the photosensors, the butt-joint pipe, and the ultraviolet lamp at the same angle are located between adjacent shielding layers; when the butt-joint pipe is separated from the suction pipe and the drainage pipe, the shielding layer shields the photosensors, the butt-joint pipe, and the ultraviolet lamp at the same angle;

[0032] The photosensor is used to detect the current intensity in real time and send the current intensity to the control panel; the control panel is used to compare the current intensity with the set standard value. If the current intensity is consistent with the standard value, a verification pass instruction is sent to the second motor; if the current intensity is inconsistent with the standard value, an adjustment instruction is sent to the second motor.

[0033] The above scheme has the following beneficial effects:

[0034] 1. In this scheme, after the centrifugal mechanism separates the peripheral blood, the stratification height is determined by the turbidity detection mechanism to determine the aggregation stratification height of the markers at different stratification heights, so as to facilitate the subsequent control of the solenoid valve to transport the magnetic beads for specific binding, and to control the magnetic separation parameters of the magnetic separation mechanism based on the stratification height at the current time, thereby ensuring the capture efficiency and purity of the markers by the magnetic beads, thereby improving the diagnostic accuracy.

[0035] 2. In this solution, the central block is moved inside the test tube to facilitate the aspiration of the centrifuge fluid at different layer heights, reducing the mixing of the centrifuge fluids at different layer heights to ensure the purity of the centrifuge fluid in the subsequent detection process, thereby ensuring the capture efficiency and purity of the magnetic beads for the markers.

[0036] 3. In this solution, during the operation and control of the solenoid valve, the magnetic beads with different surface modification layers are controlled to specifically bind to the centrifuge fluids with different layering heights, thereby reducing the waste of magnetic beads and accurately combining the corresponding magnetic beads with the centrifuge fluid, making it easy to adjust and control the working parameters as a comparative adjustment, ensuring the accurate extraction of markers in the centrifuge fluid, and thus improving the accuracy of diagnosis.

[0037] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is an axonometric diagram of an embodiment of the peripheral blood-specific early lung cancer diagnosis auxiliary device of the present invention;

[0039] Figure 2 A top view of an embodiment of the peripheral blood-specific early lung cancer diagnosis auxiliary device of the present invention;

[0040] Figure 3 for Figure 2 Schematic diagram of the cross section along the AA direction;

[0041] Figure 4 for Figure 2 Schematic diagram of the cross section along the middle BB direction;

[0042] Figure 5 for Figure 1 A schematic top view of the middle glass plate;

[0043] Figure 6 for Figure 5 A magnified schematic diagram of the local C in the middle;

[0044] Figure 7 for Figure 1 Axonometric diagram of the center rack;

[0045] Figure 8 for Figure 3 A magnified schematic diagram of the local D in the middle;

[0046] Figure 9 for Figure 4 A magnified schematic diagram of the local E in the middle;

[0047] Figure 10 for Figure 4 Schematic cross-section of the central block.

[0048] The figure marks in the drawings of the specification include: 1. outer shell; 11. bracket; 2. placement rack; 21. test tube; 22. first motor; 23. screw; 24. clamping block; 3. first electric push rod; 31. center block; 32. transfer block; 33. second motor; 34. docking tube; 35. injection cavity; 36. solenoid valve; 37. ultraviolet lamp; 38. support shaft; 39. photosensor; 4. glass plate; 41. gap; 42. recovery tank; 43. drainage tube; 5. suction tube; 51. connection port; 6. slider; 61. pull rod; 62. second electric push rod; 7. negative pressure tube; 8. shielding layer. DETAILED DESCRIPTION

[0049] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0050] In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limitations on the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0051] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0052] The following is further described in detail through specific implementation methods:

[0053] Example 1:

[0054] As attached Figures 1 to 9As shown: A peripheral blood-specific early lung cancer diagnosis auxiliary device includes a shell 1, a centrifugal mechanism for centrifugal separation of peripheral blood is provided inside the shell 1, the centrifugal mechanism includes a placement rack 2 that rotates with the shell 1, a first motor 22 is coaxially fixedly connected to the bottom of the placement rack 2, the first motor 22 is fixedly connected to the shell 1, a test tube 21 for containing peripheral blood is placed on the placement rack 2, and the centrifugal speed adjustment is a prior art and will not be repeated in this example; turbidity detection mechanisms are provided on both sides of the placement rack 2 for detecting the height of the layers of peripheral blood in the test tube 21 after centrifugation.

[0055] It also includes a control panel (not shown in the figure) electrically connected to the centrifugal mechanism and the turbidity detection mechanism, and the control panel is electrically connected to a transfer mechanism for aspirating peripheral blood in and out of the test tube 21; the transfer mechanism includes a central block 31, and the end of the central block 31 close to the test tube 21 is connected to the suction tube 5, and the end of the central block 31 away from the suction tube 5 is connected to a magnetic separation mechanism for performing magnetic field screening of magnetic beads. A number of docking tubes 34 connected to the suction tube 5 and the magnetic separation mechanism are provided in the central block 31, and the docking tubes 34 are connected to a number of injection tubes, which are used to transport magnetic beads with different surface modification layers, and the injection tubes are connected to an electromagnetic valve 36.

[0056] The turbidity detection mechanism includes a clamping block 24, which is symmetrically arranged with the placement rack 2 as the center. The clamping block 24 slides with the placement rack 2, and one end of the clamping block 24 slides with a screw rod 23. The screw rod 23 and the clamping block 24 constitute a ball screw 23 mechanism. The screw rod 23 rotates with the placement rack 2, and one end of the screw rod 23 is coaxially fixedly connected to a driving member; in this embodiment, the driving member is fixedly connected to the placement rack 2, and the driving member is a motor.

[0057] A mounting hole for placing the test tube 21 is provided at the center of the placement rack 2. A light-emitting unit for emitting infrared light and a detection unit for detecting scattered light are provided on both sides of the mounting hole. The detection unit is electrically connected to the control panel. The control panel compares the consistency of the light intensity of the scattered light before and after the current time. If they are consistent, the duration is recorded, and the layer height is marked based on the duration and the power of the driving component. If they are inconsistent, a layer mark is established and the duration is reset to zero.

[0058] The central block 31 is located above the test tube 21, and a first electric push rod 3 with adjustable extension length is fixedly connected to the top of the central block 31. The first electric push rod 3 is electrically connected to the control panel, and the end of the first electric push rod 3 away from the test tube 21 is fixedly connected to the bracket 11, and the bracket 11 is fixedly connected to the top of the outer shell 1; the suction tube 5 is located on the side of the central block 31 away from the first electric push rod 3, and the suction tube 5 is arranged circumferentially with the central block 31 as the center, and a connecting port 51 is opened on the side of the suction tube 5 away from the central block 31; a transfer block 32 is rotatably fitted in the central block 31, and the docking tube 34 is located in the transfer block 32, and the docking tube 34 is arranged circumferentially with the transfer block 32 as the center, and the docking tube 34 is located between the suction tube 5 and the magnetic suction mechanism.

[0059] A second motor 33 is coaxially fixedly connected to the top of the transfer block 32, which is also fixedly connected to the central block 31. Several injection chambers 35 are defined within the transfer block 32 for storing magnetic beads with different surface-modified layers. Injection tubes are located between the injection chambers 35 and the docking tube 34. The end of the injection tube closest to the injection chamber 35 is higher than the end farther from the injection chamber 35. This height difference allows the magnetic beads in the injection chamber 35 to fall into the docking tube 34 due to gravity, allowing them to specifically bind to the markers in the centrifuge. When the docking tube 34 is aligned with the aspiration tube 5, the aspiration tube 5 connects to the magnetic separation mechanism through the docking tube 34.

[0060] The magnetic separation mechanism includes several glass plates 4 arranged in a ring centered around the first electric push rod 3. The sides of the glass plates 4 facing away from the first electric push rod 3 are fixedly connected to the housing 1. Gaps 41 are provided between adjacent glass plates 4, connected to drainage tubes 43. The transfer mechanism also includes a negative pressure tube 7 connected to the drainage tubes 43. The end of the negative pressure tube 7 facing away from the drainage tube 43 is connected to a negative pressure device (not shown) for generating negative pressure suction. This negative pressure device is conventional and will not be described in detail in this embodiment. The side of the glass plates 4 closest to the electromagnet is higher than the side of the glass plates 4 facing away from the electromagnet.

[0061] The drainage tube 43 is fixedly connected to the fixed end of the first electric push rod 3, and the end of the drainage tube 43 away from the glass plate 4 is wrapped around the output end of the first electric push rod 3, and the drainage tube 43 is connected to the suction tube 5 through the docking tube 34. In this embodiment, the drainage tube 43 is a deformable rubber hose; electromagnets (not shown in the figure) for adjusting the magnetic field strength are provided on both sides of the glass plate 4, and the electromagnets are electrically connected to the control panel. The electromagnet is located on one side of the glass plate 4, and the end of the gap 41 away from the drainage tube 43 is connected to several water pipes. The water pipes are located on both sides of the bottom of the glass plate 4, and the water pipes are respectively connected to the recovery tank 42.

[0062] The control panel is used to control the operation of the solenoid valve 36 based on the layer height at the current moment.

[0063] The specific implementation process is as follows:

[0064] Because magnetic separation specifically binds to the supernatant or precipitate after blood centrifugation, it depends on the location of the target molecule. If the target molecule is a cellular component (such as circulating tumor cells (CTCs)), CTCs, as tumor cells, are usually present in the white blood cell layer or precipitate, not the supernatant, and the magnetic beads mainly bind to the precipitate. If the target molecule is an extracellular component (such as circulating tumor DNA, ctDNA, exosomes), ctDNA is DNA fragments released into the plasma by tumor cells and is present in the supernatant, then the magnetic beads mainly bind to the supernatant.

[0065] After the peripheral blood in the test tube 21 is centrifuged, the screw 23 is rotated by the driving member, so that the clamping block 24 constituting the ball screw 23 mechanism with the screw 23 moves at a uniform speed on the screw 23, so that the light-emitting unit and the detection unit on the clamping block 24 illuminate and receive the test tube 21. Based on the change in the consistency of the light intensity of the scattered light, it is determined whether there is a change in the suspended matter in the centrifuge at that time, so as to distinguish the boundary between the supernatant and the sediment; and then the moving speed of the clamping block 24 on the screw 23 and the duration of the recording are used to determine the height of different layers, so as to facilitate the subsequent control and processing of the connectivity of the solenoid valve 36.

[0066] During the collection of the centrifugal liquid, the first electric push rod 3 is supported by the bracket 11 to improve the stability of the first electric push rod 3 during installation; the first electric push rod 3 is then used to drive the central block 31 to move linearly, and the extension length of the first electric push rod 3 is controlled based on the change in the layer height, so that the suction tube 5 installed on the central block 31 is in contact with the centrifugal liquid in the test tube 21, thereby realizing the suction treatment of the centrifugal liquid of different layer heights in the test tube 21.

[0067] During the suction process of the suction tube 5, the second motor 33 is used to drive the transfer block 32 to rotate, so that the docking tubes 34 at different positions on the transfer block 32 are connected with the suction tube 5, reducing the mixing of centrifuge liquids at different layer heights, thereby ensuring the capture efficiency and purity of the magnetic beads for the markers.

[0068] During the process of aspirating the centrifugal liquid using the drainage tube 43, the drainage tube 43 wound around the first electric push rod 3 is connected to accommodate the movement of the central block 31 and ensure normal connection of the centrifugal liquid. The negative pressure suction generated by the negative pressure tube 7 provides power to draw the centrifugal liquid into the gap 41 inside the glass plate 4 for diffusion.

[0069] When the centrifugal liquid enters the adjacent glass plate 4, the tension of the liquid itself is used to diffuse in the gap 41 of the glass plate 4 to increase the flow area of ​​the centrifugal liquid, making it easier for the magnetic beads to move inside the glass plate 4, thereby making it easier for the magnetic field to capture the magnetic beads, causing the markers combined with the surface modification layer of the magnetic beads to gather and flow in the direction of the magnetic field. The markers are separated from the normal centrifugal liquid through the water pipes on both sides of the bottom of the glass plate 4 to ensure the capture efficiency and purity of the markers by the magnetic beads, thereby improving the accuracy of diagnosis.

[0070] At the same time, in the process of the centrifugal liquid filling the gap 41 between the adjacent glass plates 4, the suspended matter in the centrifugal liquid moves toward the side away from the electromagnet due to gravity, and the markers adsorbed by the magnetic beads overcome the height difference set by the glass plates 4 and gather to the side of the electromagnet. Then, the glass plates 4 are close to the height difference between the first electric push rod 3 and the recovery tank 42, so that the magnetic beads are separated from other suspended matter in the centrifugal liquid, thereby ensuring the capture efficiency and purity of the markers by the magnetic beads, thereby improving the diagnostic accuracy.

[0071] Example 2:

[0072] The difference from Example 1 is that a sealing mechanism for adjusting the opening size of the connecting port 51 is further provided on one side of the connecting port 51, and the sealing mechanism includes a slider 6 that slides with the center block 31, and the slider 6 is symmetrically arranged with the suction tube 5 as the center; a pull rod 61 is provided between the slider 6 and one side of the suction tube 5, one end of the pull rod 61 is hinged to the slider 6, and the other end of the pull rod 61 is hinged to the suction tube 5; a second electric push rod 62 is fixedly connected to the side of the slider 6 away from the suction tube 5, the second electric push rod 62 slides with the center block 31, and the second electric push rod 62 is electrically connected to the control panel.

[0073] The specific implementation process is as follows: Due to the different diameters of suspended matter in the supernatant and the sediment, during the process of pumping centrifuges of different layer heights, the second electric push rod 62 drives one side of the suction tube 5 to move. When the slider 6 drives the suction tube 5 to close toward the center through the pull rod 61, the opening size of the connection port 51 becomes smaller to limit the flow rate of the centrifuge through the connection port 51, so that the centrifuge in the test tube 21 is slowly sucked out to adapt to the centrifuge with a lower layer height. At the same time, the connection port 51 with a smaller opening facilitates the subsequent rapid closure process by the second electric push rod 62, thereby reducing the mixing of centrifuges of different layer heights. When the adjacent suction tube 5 is closed, the connection port 51 is closed; the second motor 33 drives the transfer block 32 to rotate so that different connecting pipes 34 can connect the suction tube 5 and the drainage tube 43. The second electric push rod 62 in different directions drives the corresponding connection port 51 to close, so that the centrifuges of different layer heights can be processed separately. At the same time, during the comparative experiment, the transfer block 32 drives the connecting tubes 34 to be connected, so that different connecting tubes 34 are mixed with different magnetic beads for specific binding treatment, thereby ensuring the capture efficiency and purity of the magnetic beads for the markers under different conditions.

[0074] When the slider 6 drives the suction tube 5 to disperse to both sides through the pull rod 61, the connecting port 51 becomes larger. Under the action of the same negative pressure suction, the flow rate of the centrifugal liquid passing through the connecting port 51 is increased, which facilitates the accelerated extraction of the centrifugal liquid with a high stratification height and improves work efficiency. At the same time, the large opening of the connecting port 51 facilitates the passage of large sediments of suspended matter, reduces possible blockage, and thus facilitates the suction of the centrifugal liquid.

[0075] Example 3:

[0076] The difference from Example 2 is that, in combination with Figure 10 As shown, the transfer block 32 is a transparent glass block, and an ultraviolet lamp 37 is fixedly connected to the side of the center block 31 close to the transfer block 32. The ultraviolet lamp 37 is electrically connected to the control panel and is located between adjacent suction pipes 5; and a plurality of shielding layers 8 are fixedly connected to the transfer block 32, and the shielding layers 8 are located between adjacent docking pipes 34.

[0077] A support shaft 38 is rotatably fitted at the center of the transfer block 32. The support shaft 38 is located at one end of the transfer block 32 close to the suction pipe 5, and the support shaft 38 is fixedly connected to the center block 31; a number of photosensors 39 are fixedly connected to the support shaft 38, and the photosensors 39 are located at the angle between adjacent suction pipes 5; and when the docking tube 34 is docked with the suction pipe 5 and the drainage pipe 43, the photosensors 39, the docking tube 34 and the ultraviolet lamp 37 at the same angle are located between adjacent shielding layers 8; when the docking tube 34 is separated from the suction pipe 5 and the drainage pipe 43, the shielding layer 8 shields the photosensors 39, the docking tube 34 and the ultraviolet lamp 37 at the same angle.

[0078] The photosensor 39 is used to detect the current intensity in real time and send the current intensity to the control panel; the control panel is used to compare the current intensity with the set standard value. If the current intensity is consistent with the standard value, a verification pass instruction is sent to the second motor 33; if the current intensity is inconsistent with the standard value, an adjustment instruction is sent to the second motor 33.

[0079] The specific implementation process is as follows: the control panel controls the activation of the ultraviolet lamp 37 to kill potential viruses in the centrifuge liquid remaining in the connecting pipe 34, thereby improving the safety of the subsequent manual cleaning process. At the same time, the shielding layer 8 is set to reduce the interference between adjacent suction pipes 5, thereby ensuring the accuracy of subsequent detection results.

[0080] The photosensor 39 is set up so that when the second motor 33 drives the transfer block 32 to rotate, the shielding layer 8 is used to confirm the light irradiation situation emitted by the ultraviolet lamp 37. The current intensity detected by the photosensor 39 is compared and processed to determine whether the photosensor 39, the docking tube 34 and the ultraviolet lamp 37 are in the same straight line, thereby determining whether the docking tube 34 is aligned with the suction tube 5 and the drainage tube 43. The second motor 33 is adjusted to ensure the accuracy of the centrifuge liquid suction process.

[0081] Example 4:

[0082] The difference from Example 3 is that the end of the glass plate 4 close to the first electric push rod 3 is higher than the end of the glass plate 4 away from the first electric push rod 3, and the negative pressure tube 7 is located at the highest point of the drainage tube 43.

[0083] The specific implementation process is as follows: during the process of suctioning by the negative pressure tube 7 on the drainage tube 43, the docking tube 34 and the suction tube 5, when the centrifuge liquid inside the drainage tube 43 passes through the highest point of the drainage tube 43, the centrifuge liquid inside falls into the glass plate 4 by gravity through the siphon principle, which facilitates the automatic suction treatment of a large volume of centrifuge liquid; it also facilitates the subsequent cleaning process, using the siphon principle to automatically suck water to clean the interior.

[0084] Example 5:

[0085] The difference from Example 4 is that the control panel is also used to enter and store the position of each suction tube 5 and the type of magnetic beads corresponding to the solenoid valve 36 at each position, and mark the second electric push rod 62 based on the position of each suction tube 5. At the same time, basic opening size addition instructions are added based on the layer marks corresponding to different layer heights. The basic opening size addition instructions are used to mark the size of the basic opening in sequence in a clockwise direction; the control panel then compares the extended length of the first electric push rod 3 with the layer height at the current time, obtains the layer mark with the extended length consistent with the layer height, and sends a start instruction to the corresponding second electric push rod 62 based on the layer mark; at the same time, the corresponding magnetic bead type is obtained based on the layer mark, and a start instruction is sent to the corresponding solenoid valve 36 based on the magnetic bead type.

[0086] For example, the corresponding suction tube 5 is marked by the change in the characteristics of the separation liquid after centrifugation of peripheral blood, so that the basic opening size is adjusted in advance based on the position change of the suction tube 5; in the process of the first electric push rod 3 pushing the central block 31 to move, the extended length and the layer height are used for comparison processing to adjust the opening size of the connecting port 51 based on the layer mark at that time, and at the same time, the corresponding solenoid valve 36 is automatically controlled based on the layer mark, so that the corresponding magnetic beads and the markers are specifically combined, reducing human manipulation, realizing intelligent processing flow, and ensuring the consistency of the magnetic beads capturing the markers.

[0087] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A peripheral blood-specific early lung cancer diagnosis auxiliary device, comprising a housing (1), a centrifugal mechanism for centrifuging peripheral blood, the centrifugal mechanism comprising a placement rack (2) rotatably engaged with the housing (1), a test tube (21) for containing peripheral blood placed on the placement rack (2); characterized in that: Turbidity detection mechanisms for detecting the height of the layers of peripheral blood after centrifugation in the test tube (21) are provided on both sides of the placement rack (2); It also includes a control panel electrically connected to the centrifugal mechanism and the turbidity detection mechanism, and the control panel is electrically connected to a transfer mechanism for aspirating peripheral blood in and out of the test tube (21); The transfer mechanism includes a central block (31), one end of the central block (31) close to the test tube (21) is connected to a suction tube (5), and one end of the central block (31) away from the suction tube (5) is connected to a magnetic separation mechanism for performing magnetic field screening of magnetic beads. The central block (31) is provided with a plurality of butt joints (34) connected to the suction tube (5) and the magnetic separation mechanism. The butt joints (34) are connected to a plurality of injection tubes, and the injection tubes are used to transport magnetic beads with different surface modification layers. The injection tubes are connected to electromagnetic valves (36). The control panel is used to control the solenoid valve (36) to work based on the layer height at the current moment.

2. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 1, characterized in that: The turbidity detection mechanism comprises a clamping block (24), the clamping block (24) is symmetrically arranged with the placement rack (2) as the center, the clamping block (24) and the placement rack (2) are slidably matched, one end of the clamping block (24) is slidably matched with a screw rod (23), the screw rod (23) and the placement rack (2) are rotationally matched, and one end of the screw rod (23) is coaxially fixedly connected with a driving member; A mounting hole for placing a test tube (21) is provided at the center of the placement rack (2), and a light-emitting unit for emitting infrared light and a detection unit for detecting scattered light are provided on both sides of the mounting hole, and the detection unit is electrically connected to the control panel; The control panel compares the consistency of the scattered light intensity before and after the current time. If they are consistent, the duration is recorded and the layer height is marked based on the duration and the power of the driving component. If they are inconsistent, a layer mark is established and the duration is reset to zero.

3. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 2, characterized in that: The central block (31) is located above the test tube (21), and a first electric push rod (3) with an adjustable extension length is fixedly connected to the top of the central block (31), the first electric push rod (3) is electrically connected to the control panel, and an end of the first electric push rod (3) away from the test tube (21) is fixedly connected to a bracket (11), and the bracket (11) is fixedly connected to the top of the housing (1); The suction pipe (5) is located on a side of the central block (31) away from the first electric push rod (3), the suction pipe (5) is arranged circumferentially with the central block (31) as the center, and a connecting port (51) is opened on the side of the suction pipe (5) away from the central block (31); a transfer block (32) is rotatably matched in the central block (31), a butt joint (34) is located in the transfer block (32), the butt joint (34) is arranged circumferentially with the transfer block (32) as the center, and the butt joint (34) is located between the suction pipe (5) and the magnetic attraction mechanism; A second motor (33) is coaxially fixedly connected to the top of the transfer block (32), and the second motor (33) is fixedly connected to the central block (31); a plurality of injection cavities (35) for storing magnetic beads with different surface modification layers are opened in the transfer block (32), and the injection tube is located between the injection cavity (35) and the docking tube (34); When the butt joint pipe (34) is aligned with the suction pipe (5), the suction pipe (5) is connected to the magnetic separation mechanism through the butt joint pipe (34).

4. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 3, characterized in that: The magnetic separation mechanism comprises a plurality of glass plates (4), the glass plates (4) being arranged in a ring shape with the first electric push rod (3) as the center, and the side of the glass plates (4) away from the first electric push rod (3) being fixedly connected to the housing (1); A gap (41) is provided between adjacent glass plates (4), and the gap (41) is connected to a drainage pipe (43); the transfer mechanism further comprises a negative pressure pipe (7) connected to the drainage pipe (43), and one end of the negative pressure pipe (7) away from the drainage pipe (43) is connected to a negative pressure machine for generating negative pressure suction; The drainage tube (43) is fixedly connected to the fixed end of the first electric push rod (3), the end of the drainage tube (43) away from the glass plate (4) is wound around the output end of the first electric push rod (3), and the drainage tube (43) is connected to the suction tube (5) through the docking tube (34); Electromagnets for adjusting the magnetic field strength are provided on both sides of the glass plate (4), and the electromagnets are electrically connected to a control panel. The electromagnets are located on one side of the glass plate (4). One end of the gap (41) away from the drainage pipe (43) is connected to a plurality of water pipes. The water pipes are located on both sides of the bottom of the glass plate (4), and the water pipes are respectively connected to a recovery tank (42).

5. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 4, characterized in that: A sealing mechanism for adjusting the opening size of the connecting port (51) is also provided on one side of the connecting port (51). The sealing mechanism includes a slider (6) that is slidably engaged with the central block (31). The slider (6) is symmetrically arranged with the suction pipe (5) as the center. A pull rod (61) is provided between the slider (6) and one side of the suction pipe (5), one end of the pull rod (61) is hinged to the slider (6), and the other end of the pull rod (61) is hinged to the suction pipe (5); a second electric push rod (62) is fixedly connected to the side of the slider (6) away from the suction pipe (5), the second electric push rod (62) is slidably matched with the center block (31), and the second electric push rod (62) is electrically connected to the control panel.

6. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 5, characterized in that: The transfer block (32) is a transparent glass block. An ultraviolet lamp (37) is fixedly connected to one side of the central block (31) close to the transfer block (32). The ultraviolet lamp (37) is electrically connected to the control panel. The ultraviolet lamp (37) is located between adjacent suction pipes (5). In addition, a plurality of shielding layers (8) are fixedly connected to the transfer block (32). The shielding layers (8) are located between adjacent butt-jointed pipes (34).

7. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 6, characterized in that: An end of the glass plate (4) close to the first electric push rod (3) is higher than an end of the glass plate (4) away from the first electric push rod (3), and the negative pressure tube (7) is located at the highest point of the drainage tube (43).

8. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 7, characterized in that: The side of the glass plate (4) close to the electromagnet is higher than the side of the glass plate (4) away from the electromagnet.

9. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 8, characterized in that: The control panel is also used to record and store the position of each suction tube (5) and the type of magnetic beads corresponding to the electromagnetic valve (36) at each position, mark the second electric push rod (62) based on the position of each suction tube (5), and send a basic opening size addition instruction based on the layer mark corresponding to different layer heights. The basic opening size addition instruction is used to mark the size of the basic opening in sequence in a clockwise direction. The control panel then compares the extended length of the first electric push rod (3) with the layer height at the current time, obtains a layer mark that is consistent with the extended length and the layer height, and sends a start instruction to the corresponding second electric push rod (62) based on the layer mark; at the same time, the control panel obtains the corresponding magnetic bead type based on the layer mark, and sends a start instruction to the corresponding solenoid valve (36) based on the magnetic bead type.

10. The peripheral blood-specific early lung cancer diagnosis auxiliary device according to claim 9, characterized in that: A support shaft (38) is rotatably fitted at the center of the transfer block (32), the support shaft (38) being located at one end of the transfer block (32) close to the suction pipe (5), and the support shaft (38) being fixedly connected to the center block (31); A plurality of light-sensitive sensors (39) are fixedly connected to the support shaft (38), and the light-sensitive sensors (39) are located at an angle between adjacent suction pipes (5); and when the butt-joint pipe (34) is butt-jointed with the suction pipe (5) and the drainage pipe (43), the light-sensitive sensors (39), the butt-joint pipe (34) and the ultraviolet lamp (37) at the same angle are located between adjacent shielding layers (8); and when the butt-joint pipe (34) is separated from the suction pipe (5) and the drainage pipe (43), the shielding layer (8) shields the light-sensitive sensors (39), the butt-joint pipe (34) and the ultraviolet lamp (37) at the same angle. The light-sensitive sensor (39) is used to detect the current intensity in real time and send the current intensity to the control panel; the control panel is used to compare the current intensity with a set standard value, and if the current intensity is consistent with the standard value, a verification pass instruction is sent to the second motor (33); if the current intensity is inconsistent with the standard value, an adjustment instruction is sent to the second motor (33).

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