Method for improving colistin E wastewater treatment capacity

Abstract

The invention discloses a method for improving colistin E wastewater treatment capacity, and belongs to the technical field of wastewater treatment. The method specifically comprises the following steps: A, putting activated sludge into a reactor; and B, taking the colistin E production wastewater additionally added with cyclic diguanylic acid to flow through the reactor at a certain organic load. The colistin E production wastewater has a multi-aspect toxic effect on microorganisms in activated sludge, so that the growth of the microorganisms is inhibited, and cyclic diguanylate is a key regulatory factor for synthesizing an extracellular matrix and can activate a series of genes participating in the synthesis of components such as exopolysaccharides and proteins, so that the microorganisms can generate a large amount of extracellular matrix, and the growth of the microorganisms is inhibited. The extracellular matrixes are main components forming the biological membrane and can provide a protective and stable living environment for microorganisms. Experiments prove that the cyclic diguanylate is additionally added into the colistin E production wastewater, so that the wastewater treatment capacity can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of wastewater treatment, and in particular to a method for improving the treatment capacity of colistin wastewater. Background Art

[0002] Colistin E is a polypeptide antibiotic primarily used to prevent and treat infections caused by sensitive bacteria and promote growth in livestock and poultry. Colistin E binds to free phosphates in cell membrane lipoproteins, reducing cell membrane surface tension and increasing permeability, leading to cytoplasmic efflux and cell death. Colistin E has a strong inhibitory effect on Gram-negative bacteria (particularly Escherichia coli, Salmonella, Pseudomonas aeruginosa, Proteus, and Haemophilus influenzae), but has no effect on Gram-positive bacteria (except Staphylococcus aureus and hemolytic Streptococci) or fungi.

[0003] The production process of colistin E generates a large amount of wastewater, requiring significant manpower, material resources, and financial resources to treat. When treatment capacity is limited, production capacity must be sacrificed to meet discharge standards. Otherwise, adverse environmental impacts will result. Therefore, improving wastewater treatment capacity is crucial.

[0004] Colistin E production wastewater has multiple toxic effects on microorganisms in activated sludge. For example, colistin can disrupt bacterial cell membrane structures, causing the leakage of intracellular substances, affecting cellular metabolic activity, and thus inhibiting microbial growth and reproduction. Wastewater may contain high concentrations of nutrients such as nitrogen and phosphorus, as well as some difficult-to-degrade organic substances such as polysaccharides and proteins, which can lead to nutritional imbalances in microorganisms, affect their normal metabolic pathways, and inhibit their growth. Cyclic diguanylate (CDM) is a key regulatory factor in the synthesis of the extracellular matrix. It activates a series of genes involved in the synthesis of extracellular polysaccharides, proteins, and other components, allowing microorganisms to produce large amounts of extracellular matrix. This extracellular matrix is ​​the main component of biofilms and can provide protection and a stable living environment for microorganisms. Therefore, the introduction of CDM into wastewater treatment is beneficial for maintaining biological activity and thus improving wastewater treatment capacity. Summary of the Invention

[0005] Purpose of the invention: The purpose of the present invention is to provide a method for improving the wastewater treatment capacity of colistin E, thereby improving the wastewater treatment capacity.

[0006] Technical solution: To solve the above technical problems, according to one aspect of the present invention, more specifically, a method for improving the treatment capacity of colistin wastewater, specifically comprising the following steps:

[0007] A. Take activated sludge and place it in the reactor;

[0008] B. Take the wastewater of colistin E with additional cyclic diguanylate and add 0.1-20kgCOD / (m 3*d) The organic load flows through the reactor.

[0009] Furthermore, the temperature of the reactor in step A is controlled at 35±5°C.

[0010] Furthermore, the temperature of the reactor in step A is controlled at 35±2°C.

[0011] Furthermore, the organic load of the wastewater produced by colistin E in step B is in the range of 1-10 kgCOD / (m 3 *d).

[0012] Furthermore, the organic load of the wastewater produced by colistin E in step B is in the range of 3-7 kgCOD / (m 3 *d).

[0013] Furthermore, the organic load range of the colistin production wastewater in step B is 5 kgCOD / (m 3 *d).

[0014] Furthermore, the concentration of the additional cyclic diguanylate in step B is 0.1-10 μM.

[0015] Furthermore, the concentration of the additional cyclic diguanylate in step B is 0.5-2 μM.

[0016] Furthermore, in step B, the flow direction of the colistin E production wastewater is bottom inlet and top outlet, and the fermentation method is anaerobic fermentation.

[0017] Beneficial Effects: The key technology of this invention involves placing activated sludge in a reactor and then passing colistin production wastewater, supplemented with cyclic diguanylate (cDGP), through the reactor at a specific organic load. The addition of cDGP to the colistin production wastewater effectively improves wastewater treatment capacity. Experimental studies have shown that this method improves COD removal by 8.5% compared to conventional technologies. DETAILED DESCRIPTION

[0018] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below with reference to specific embodiments.

[0019] Example 1

[0020] Activated sludge was placed in the reactor and the temperature was controlled at 35±2℃. The wastewater from colistin E production was taken at a temperature of 5kgCOD / (m 3 *d) organic load flows through the reactor. Colistin E production wastewater flows from the bottom into the reactor and out from the top, and the fermentation method is anaerobic.

[0021] The test results on the fifteenth day of the reaction are shown in Table 1:

[0022] Table 1: Test results of Example 1

[0023] name Influent COD (mg / L) Outlet COD (mg / L) COD removal rate Example 1 3501 812 76.8％

[0024] Example 2

[0025] The difference from Example 1 is that cyclic diguanylate is additionally added to the colistin E production wastewater at a concentration of 0.5 μM.

[0026] The test results on the fifteenth day of the reaction are shown in Table 2:

[0027] Table 2: Test results of Example 2

[0028] name Influent COD (mg / L) Outlet COD (mg / L) COD removal rate Example 2 3510 721 79.5％

[0029] Example 3

[0030] The difference from Example 2 is that the concentration of the additional added cyclic diguanylate is 1 μM.

[0031] The test results on the fifteenth day of the reaction are shown in Table 3:

[0032] Table 3: Test results of Example 3

[0033] name Influent COD (mg / L) Outlet COD (mg / L) COD removal rate Example 3 3505 647 81.5％

[0034] Example 4

[0035] The difference from Example 2 is that the concentration of the additional added cyclic diguanylate is 1.5 μM.

[0036] The test results on the fifteenth day of the reaction are shown in Table 4:

[0037] Table 4: Test results of Example 4

[0038] name Influent COD (mg / L) Outlet COD (mg / L) COD removal rate Example 4 3515 588 83.3％

[0039] Example 5

[0040] The difference from Example 2 is that the concentration of the additional added cyclic diguanylate is 2 μM.

[0041] The test results on the fifteenth day of the reaction are shown in Table 5:

[0042] Table 5: Test results of Example 5

[0043] name Influent COD (mg / L) Outlet COD (mg / L) COD removal rate Example 5 3506 616 82.4％

[0044] The above examples demonstrate that the addition of cyclic diguanylate (cDGP) to colistin production wastewater improves COD removal. Example 4 achieves the greatest improvement, achieving an 8.5% improvement relative to Example 1. These examples demonstrate that the present invention can improve colistin wastewater treatment capacity.

[0045] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A method for improving the treatment capacity of colistin wastewater, characterized in that: The specific steps include: A. Take activated sludge and place it in the reactor; B. Take the wastewater of colistin E with additional cyclic diguanylate and add 0.1-20kgCOD / (m 3 *d) The organic load flows through the reactor.

2. The method for improving the treatment capacity of colistin wastewater according to claim 1, wherein: The temperature of the reactor in step A is controlled at 35±5°C.

3. The method for improving the wastewater treatment capacity of Colistin E according to claim 2, characterized in that: The temperature of the reactor in step A is controlled at 35±2°C.

4. The method for improving the colistin wastewater treatment capacity according to claim 1, wherein: The organic load of the wastewater produced by colistin E in step B is in the range of 1-10 kgCOD / (m 3 *d).

5. The method for improving the colistin wastewater treatment capacity according to claim 4, characterized in that: The organic load of the wastewater produced by colistin E in step B is in the range of 3-7 kgCOD / (m 3 *d).

6. The method for improving the colistin wastewater treatment capacity according to claim 5, characterized in that: The organic load range of the wastewater produced by colistin E in step B is 5 kgCOD / (m 3 *d).

7. The method for improving the colistin wastewater treatment capacity according to claim 1, characterized in that: The concentration of the additional cyclic diguanylate in step B is 0.1-10 μM.

8. The method for improving the colistin wastewater treatment capacity according to claim 7, characterized in that: The concentration of the additional cyclic diguanylate in step B is 0.5-2 μM.

9. The method for improving the colistin wastewater treatment capacity according to claim 1, characterized in that: In the step B, the flow direction of the colistin E production wastewater is water inlet at the bottom and water outlet at the top, and the fermentation method is anaerobic fermentation.