Production device for synthesizing succinic acid by using carbon dioxide

Abstract

The invention discloses a production device for synthesizing succinic acid by using carbon dioxide, and particularly relates to the technical field of biochemical engineering, and the production device comprises a CO2 pretreatment unit which comprises a CO2 compressor, a gas purity detection device and a deoxidizing and desulfurizing module and is used for purifying CO2 in industrial waste gas to reach an industrial-grade application standard; the multi-stage fermentation reaction unit is composed of at least three anaerobic fermentation tanks which are connected in series, each tank is provided with an improved multi-stage vortex diffuser and an ultra-micro bubble generator, and a swirling flow field is formed through tangential air inlet to realize nanoscale bubble dispersion. According to the invention, CO2 in industrial waste gas can be purified to an industrial application standard through the CO2 pretreatment unit, the utilization rate of CO2 is increased, waste can be turned into wealth, waste gas emission is reduced, environmental protection benefits are achieved, the tail gas recycling module realizes efficient recycling of CO2 through a gas purification and mixed recycling technology, raw material consumption is remarkably reduced, and the production cost is reduced. And the production cost is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of biochemical engineering, and in particular to a production device for synthesizing succinic acid by utilizing carbon dioxide. Background Art

[0002] Traditional fermentation technology is one of the oldest food processing methods in human history, dating back thousands of years (e.g., winemaking and sauce-making in ancient China, beer brewing in ancient Egypt). Its core principle is to utilize the metabolic activity of natural microorganisms (or preserved fermentation products, such as koji and old dough) to convert carbohydrates and proteins in raw materials into products needed by humans. In traditional fermentation, CO2 is a key product of microbial metabolism. In traditional fermentation, the solubility of CO2 in water is inherently low, and the traditional fermentation system has no measures to enhance dissolution. The contact area of ​​CO2 in the form of large bubbles with the fermentation liquid is small and the residence time is short, resulting in most of the CO2 not being dissolved and escaping directly in the form of gas. Summary of the Invention

[0003] The purpose of the present invention is to provide a production device for synthesizing succinic acid using carbon dioxide to solve the above-mentioned shortcomings in the technology.

[0004] In order to achieve the above object, the present invention provides the following technical solution: a production device for synthesizing succinic acid using carbon dioxide, comprising: CO2 pretreatment unit: includes a CO2 compressor, a gas purity detection device, and a deoxidation and desulfurization module, used to purify CO2 in industrial waste gas to industrial-grade application standards; Multi-stage fermentation reaction unit: It consists of at least three anaerobic fermentation tanks connected in series. The tank body is equipped with an improved multi-stage vortex diffuser and ultra-fine bubble generator. The cyclone field is formed by tangential air intake to achieve nano-level bubble dispersion. Pulse neutralizer addition module: Integrates alkaline storage tank, pH monitoring system and intelligent pump group, dynamically switches the neutralizer injection mode according to the changes in the pH of the fermentation liquid; Online product separation unit: Integrates electrodialysis membrane and crystallizer to achieve continuous product separation and catalyst regeneration; Artificial Intelligence Control Center: Real-time monitoring of reaction parameters based on spectral analysis, and optimization of process parameter regulation through dynamic models.

[0005] Preferably, the improved multi-stage vortex diffuser adopts a composite guide structure, including a microporous filter element and an angle-adjustable spiral guide plate, which significantly prolongs the gas residence time and improves the CO2 dissolution efficiency by optimizing the gas flow path.

[0006] Preferably, the workflow of the online product separation unit includes: achieving ion selective retention by electrodialysis under specific pH conditions, converting the calcium salt product into high-purity crystals through chemical conversion, and achieving catalyst recycling through regeneration treatment.

[0007] Preferably, the dynamic model of the artificial intelligence control center includes empirical parameters and real-time data fusion algorithm, and achieves precise control of CO2 concentration through periodic parameter adjustment to maintain the dynamic balance of the reaction system.

[0008] Preferably, the fermentation tank is equipped with an intelligent temperature control system and a pressure adaptive adjustment device to maintain high solubility of CO2 through precise temperature control and dynamic pressure management, thereby ensuring the stability of the reaction system.

[0009] Preferably, a specific genetically engineered strain is used for fermentation production, the culture medium uses an optimized ratio of carbon source and nutrients, and efficient conversion is achieved by controlling the inoculation amount.

[0010] Preferably, a tail gas recycling module is provided to achieve efficient recycling of CO2 through gas purification and mixed reuse technology, thereby significantly reducing raw material consumption.

[0011] In the above technical solution, the technical effects and advantages provided by the present invention are: 1. The CO2 pretreatment unit can purify the CO2 in industrial waste gas to industrial-grade application standards, which not only improves the utilization rate of CO2, but also turns waste into treasure, reduces waste gas emissions, and has environmental benefits. The tail gas recycling module realizes the efficient recycling of CO2 through gas purification and mixed reuse technology, significantly reducing raw material consumption and production costs.

[0012] 2. The improved multi-stage vortex diffuser of the multi-stage fermentation reaction unit adopts a composite flow-guiding structure, which can significantly prolong the gas residence time and improve the CO2 dissolution efficiency. The fermentation tank is equipped with an intelligent temperature control system and a pressure adaptive adjustment device, which can maintain the high solubility of CO2 and solve the problem of CO2 dissolution difficulty in traditional fermentation.

[0013] 3. Specific genetically engineered strains are used for fermentation production, and the culture medium uses an optimized ratio of carbon source and nutrients. Efficient conversion is achieved by controlling the inoculation amount, which helps to improve the yield and quality of succinic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments described in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0015] Figure 1 It is a schematic diagram of the process of the present invention; Figure 2 Schematic diagram of the multi-stage fermentation reaction unit of the present invention; Figure 3 This is a schematic diagram of the framework of the present invention; Figure 4 Schematic diagram of the CO2 pretreatment unit of the present invention. DETAILED DESCRIPTION

[0016] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0017] The present invention provides Figures 1 to 4 A production device for synthesizing succinic acid using carbon dioxide is shown, comprising: CO2 pretreatment unit: includes a CO2 compressor, a gas purity detection device, and a deoxidation and desulfurization module, used to purify CO2 in industrial waste gas to industrial-grade application standards; Multi-stage fermentation reaction unit: It consists of at least three anaerobic fermentation tanks connected in series. The tank body is equipped with an improved multi-stage vortex diffuser and ultra-fine bubble generator. The cyclone field is formed by tangential air intake to achieve nano-level bubble dispersion. Pulse neutralizer addition module: Integrates alkaline storage tank, pH monitoring system and intelligent pump group, dynamically switches the neutralizer injection mode according to the changes in the pH of the fermentation liquid; Online Product Separation Unit: Integrates electrodialysis membranes and crystallizers to achieve continuous product separation and catalyst regeneration. The online product separation unit integrates electrodialysis membranes and crystallizers to achieve continuous product separation and catalyst regeneration. Its workflow enables ion-selective retention under specific pH conditions, converting calcium salt products into high-purity crystals and allowing catalyst recycling, improving production efficiency and reducing costs. Artificial Intelligence Control Center: Based on spectral analysis, the reaction parameters are monitored in real time, and the process parameter regulation is optimized through dynamic models. The pulsed neutralizer addition module can dynamically switch the neutralizer injection mode according to the changes in the pH of the fermentation liquid. The artificial intelligence control center monitors the reaction parameters in real time based on spectral analysis, and optimizes the process parameter regulation through dynamic models. It can accurately control the CO2 concentration and maintain the dynamic balance of the reaction system.

[0018] The improved multi-stage vortex diffuser adopts a composite guide structure, including a microporous filter element and an angle-adjustable spiral guide plate. By optimizing the gas flow path, it significantly prolongs the gas residence time and improves the CO2 dissolution efficiency.

[0019] The workflow of the online product separation unit includes: achieving ion selective retention through electrodialysis under specific pH conditions, converting the calcium salt product into high-purity crystals through chemical conversion, and achieving catalyst recycling through regeneration treatment.

[0020] The dynamic model of the artificial intelligence control center includes empirical parameters and real-time data fusion algorithm, which achieves precise control of CO2 concentration through periodic parameter adjustment to maintain the dynamic balance of the reaction system.

[0021] The fermentation tank is equipped with an intelligent temperature control system and a pressure adaptive adjustment device. Through precise temperature control and dynamic pressure management, it maintains the high solubility of CO2 and ensures the stability of the reaction system.

[0022] Specific genetically engineered strains are used for fermentation production, and the culture medium uses an optimized ratio of carbon source and nutrients, and efficient conversion is achieved by controlling the inoculation amount.

[0023] An exhaust gas recycling module is set up to achieve efficient recycling of CO2 through gas purification and mixed reuse technology, significantly reducing raw material consumption.

[0024] 1. CO2 pretreatment unit 1. Molecular sieve adsorption desulfurization Adsorption selectivity: The adsorption of sulfur-containing compounds by molecular sieves follows the principles of "molecular size matching" and "polarity difference". For example: Hydrogen sulfide (H2S): The molecular sieve pore size is highly compatible with H2S molecules, and the adsorption capacity can reach 5-8mmol / g.

[0025] Mercaptan (RSH): Because it contains polar -SH groups, it is easily adsorbed preferentially by highly polar molecular sieves (such as 5A and 13X).

[0026] Carbon disulfide (CS2): non-polar and large molecular weight, low adsorption efficiency (needs to be combined with high temperature regeneration process).

[0027] Scope of application: Suitable for deep desulfurization scenarios with low concentration of sulfides (<100ppm). The regeneration cycle is recommended to be 48 hours.

[0028] 2. Membrane filtration to remove particulate matter Pore ​​size and retention effect: Microfiltration membrane (0.1-10μm): intercepts suspended particles (such as catalyst dust), flux >100L / m 2 ·h.

[0029] Ultrafiltration membrane (1-100nm): removes colloidal substances and requires regular backwashing (cycle 6-8 hours).

[0030] Material selection: Polyvinylidene fluoride (PVDF) membrane has better corrosion resistance than polysulfone (PS) and is suitable for acidic gas environments.

[0031] 2. Multi-stage fermentation reaction unit 1. Improved multi-stage vortex diffuser Composite diversion structure: Microporous filter element: Made of 316L stainless steel sintered mesh (pore size 5-20μm), laser cutting process ensures porosity >40%.

[0032] Spiral guide vane: Made of 304 stainless steel, with an adjustable angle range of 15°-30° and an accuracy of ±0.1° achieved through CNC precision machining.

[0033] Optimization effect: When the tangential inlet flow velocity is 2m / s, the proportion of nanobubbles (<100nm) increases to 85%, and the CO2 dissolution efficiency increases by 40%.

[0034] The residence time is extended to 120 seconds, which promotes the metabolic rate of microorganisms (25% higher than traditional jet diffusers).

[0035] 3. Intelligent temperature control and pressure regulation system 1. Control algorithm and feedback mechanism Dynamic PID control: The temperature sensor (Pt100) collects data in real time with an error of <±0.5℃.

[0036] The pressure regulating valve (pneumatic diaphragm valve) has a response time of <0.5 seconds and maintains the CO2 solubility (solubility ≈0.98 g / L at 20°C).

[0037] Interlock protection: When the pressure exceeds 1.2MPa, the safety valve will automatically open and reduce the intake flow.

[0038] 4. Pulse neutralizer addition module 1. pH monitoring and control Electrode maintenance: The glass electrode has a lifespan of approximately 1-2 years and requires weekly calibration with pH 4.01 / 7.00 buffer.

[0039] Ultrasonic cleaning (frequency 28kHz) can remove biofilm on the electrode surface.

[0040] Intelligent pump group parameters: Servo motor driven, flow control accuracy ±0.5%, response time <0.2 seconds.

[0041] The pulse mode (0.5 L / min, 30 s interval) can reduce the excessive addition of NaOH (byproduct acetic acid is reduced to below 2%).

[0042] 5. Online product separation unit 1. Electrodialysis membrane performance Materials and parameters: Cation exchange membrane (such as Neosepta CPS) has a rejection rate of >98% and a current density of 0.8A / cm 2 .

[0043] Anti-pollution coating (PTFE modified) extends life to 3000 hours.

[0044] Crystallization process optimization: The cooling rate is 1-2°C / min and the stirring speed is 500-800rpm, and crystals with a purity of >99.9% can be obtained.

[0045] 6. Artificial Intelligence Control Center 1. Dynamic model and parameter adjustment Data fusion algorithm: Historical data (CO2 concentration fluctuations during the fermentation cycle) and real-time data (pH, temperature) are fused through Kalman filtering, reducing the error by 30%.

[0046] Periodic adjustment strategy: During the logarithmic growth phase: fine-tune the air inlet pressure every 2 hours (±0.05 MPa).

[0047] Stabilization period: Extended to 8 hours for adjustment, with the amplitude controlled within ±0.1MPa.

[0048] 7. Tail gas recycling module 1. Condensation and mixed reuse Condensation process: Using shell and tube condenser (-20℃, refrigerant R22), CO2 recovery rate is >95%.

[0049] Economic analysis: The cost of recycling CO2 per ton is reduced by 40%, and annual emissions are reduced by 1,200 tons (equivalent to planting 65,000 trees).

[0050] Example 1: Operation process of a full-function production device 1.CO2 pretreatment unit Parameters: Inlet pressure 0.8MPa, purity detection threshold ≥99.5%, O2 content after deoxidation and desulfurization <5ppm.

[0051] Process: After industrial waste gas is pressurized by a compressor, it is desulfurized by molecular sieve adsorption and particulate matter is removed by membrane filtration.

[0052] 2. Multi-stage fermentation reaction unit Structure: 3 fermentation tanks in series (volume 5m3 / tank), equipped with spiral guide vanes (inclination angle 30°) and microporous filter elements (pore size 5μm).

[0053] Operation: The tangential inlet gas velocity is 2 m / s, and the swirl field disperses the CO2 into nanobubbles (diameter < 100 nm), with the residence time extended to 120 seconds.

[0054] Strain: Genetically engineered strain E. coli W3110 (overexpressing the succinate dehydrogenase gene).

[0055] 3. Pulse neutralizer addition module Control logic: pH monitoring frequency 1 Hz, when pH < 5.5, start pulsed NaOH injection (flow rate 0.5 L / min, pulse interval 30 s).

[0056] 4. Online product separation unit Electrodialysis conditions: voltage 1.2 V, current density 50 A / m 2 , selectively retaining succinate ions at pH 3.0.

[0057] Crystallization process: Add CaCl2 solution (concentration 10%), cool and crystallize (0-5℃) to obtain calcium succinate with a purity of >99%, and then regenerate the catalyst through acid hydrolysis.

[0058] 5. AI Control Center Model input: Spectral analysis to monitor CO2 concentration (error ±0.5%), temperature (±0.1°C), and pressure (±0.01MPa) in real time.

[0059] Dynamic adjustment: Update the diffuser speed (±5rpm) and air flow rate (±10%) every 30 minutes to maintain the CO2 concentration in the reaction system in the range of 15-20%.

[0060] 6. Exhaust gas recirculation module Recovery rate: Unreacted CO2 is condensed (-20℃) and compressed and refluxed to the pretreatment unit, with a recycling rate of 98%.

[0061] Results: The succinic acid yield was 82.3 g / L, CO2 consumption was 0.8 kg / kg product, and energy consumption was reduced by 40% compared with the traditional process.

[0062] Comparative Example 1: Fermentation system without multi-stage vortex diffuser Difference: The fermenter is only equipped with a common jet diffuser, and the gas residence time is only 40 seconds.

[0063] result: The CO2 dissolution efficiency dropped to 70%, and the succinic acid yield dropped to 58 g / L.

[0064] The residual CO2 content in the exhaust gas increases to 15%, requiring additional adsorption treatment.

[0065] Comparative Example 2: Manually Adjusting Neutralizer Mode Difference: Relies on manual timed addition of NaOH (fixed dose of 5L every 2 hours).

[0066] result: The pH fluctuated between 5.0 and 6.5, and the by-product acetic acid content increased to 5%.

[0067] The fermentation cycle was extended by 20% and the product purity dropped to 95%.

[0068] Comparative Example 3: Intermittent product separation process Difference: Centrifugal separation is used instead of electrodialysis-crystallization.

[0069] result: The catalyst recovery rate is only 65%, and the equipment cleaning frequency has increased three times.

[0070] The purity of succinic acid dropped to 92% and energy consumption increased by 25%.

[0071] The data comparison table is as follows:

[0072] Data measurement method 1. Gas parameter measurement CO2 purity and pressure Purity: Analyzed by gas chromatography (GC) or infrared spectroscopy (FTIR), with an error range of ±0.5%.

[0073] Pressure: Use a high-precision pressure sensor (such as strain gauge or capacitive) with an accuracy of ±0.01MPa.

[0074] O2 content: Through electrochemical oxygen sensor or zirconium oxide sensor, detection limit <5ppm.

[0075] 2. Dynamic monitoring of the fermentation process Gas residence time Method: Tracer method (such as fluorescent labeled gas) combined with online infrared detector to record diffusion time with an error of ±5%.

[0076] CO2 dissolution efficiency Methods: After offline sampling, the CO2 concentration in the solution was determined by Karl Fischer method or conductivity method, and the efficiency was calculated based on mass balance.

[0077] 3. Biological reaction parameters Strain activity and metabolites Strain verification: Confirm gene expression of genetically engineered strains (such as E. coli W3110) by gene sequencing or PCR.

[0078] Succinic acid yield: The product concentration in the fermentation broth was monitored in real time using high performance liquid chromatography (HPLC) or a biosensor with an error of ±2%.

[0079] 4. Separation and purification process Electrodialysis conditions Voltage and current density: recorded by an electrochemical workstation or a customized current-voltage monitoring system with an accuracy of ±0.5%.

[0080] Crystal purity Methods: The crystal structure was verified by X-ray diffraction (XRD) or thermogravimetric analysis (TGA), and the impurity content was determined by HPLC (error ±0.5%).

[0081] 5. Energy consumption and recovery rate Energy consumption calculation Method: Install an electric energy meter (accuracy ±0.5%) to record the operating power of the equipment and calculate the total energy consumption based on the operating time.

[0082] CO2 recovery rate Method: Calculate the CO2 mass flow rate difference between the condenser outlet and inlet, with an error of ±1%.

[0083] 6. Comparative Experimental Control Manually adjusted contrast pH fluctuation: Continuously record using a pH electrode (accuracy ± 0.01) and calculate the standard deviation.

[0084] By-product detection: The acetic acid content was analyzed by GC-MS with an error of ±0.2%.

[0085] 7. Catalyst recovery rate Method: After centrifugation, the amount of catalyst residue was determined by gravimetric method or ICP-OES (inductively coupled plasma optical emission spectroscopy) with an error of ±1%.

[0086] Measurement tools and standards Real-time monitoring: sensors (pH, temperature, pressure), spectrometer (CO2 concentration), flow meter (gas / liquid).

[0087] Offline analysis: HPLC, GC, ICP-OES, XRD and other laboratory equipment.

[0088] Error control: Data reliability is ensured through multiple parallel experiments (n≥3) and calibration (NIST standard materials).

[0089] Summarize All data are collected in real time by online sensors and combined with offline laboratory analysis. Key parameters (such as yield and purity) are verified using international standard methods (such as ASTM D5370) to ensure the accuracy and repeatability of the results.

[0090] The above description is merely illustrative of certain exemplary embodiments of the present invention. It goes without saying that those skilled in the art will be able to modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the claims.

Claims

1. A production device for synthesizing succinic acid using carbon dioxide, characterized in that: include: CO2 pretreatment unit: includes a CO2 compressor, a gas purity detection device, and a deoxidation and desulfurization module, used to purify CO2 in industrial waste gas to industrial-grade application standards; Multi-stage fermentation reaction unit: It consists of at least three anaerobic fermentation tanks connected in series. The tank body is equipped with an improved multi-stage vortex diffuser and ultra-fine bubble generator. The cyclone field is formed by tangential air intake to achieve nano-level bubble dispersion. Pulse neutralizer addition module: Integrates alkaline storage tank, pH monitoring system and intelligent pump group, dynamically switches the neutralizer injection mode according to the changes in the pH of the fermentation liquid; Online product separation unit: Integrates electrodialysis membrane and crystallizer to achieve continuous product separation and catalyst regeneration; Artificial Intelligence Control Center: Real-time monitoring of reaction parameters based on spectral analysis, and optimization of process parameter regulation through dynamic models.

2. The production device for synthesizing succinic acid using carbon dioxide according to claim 1, characterized in that: The improved multi-stage vortex diffuser adopts a composite guide structure, including a microporous filter element and an angle-adjustable spiral guide plate. By optimizing the gas flow path, it significantly prolongs the gas residence time and improves the CO2 dissolution efficiency.

3. The production device for synthesizing succinic acid using carbon dioxide according to claim 1, characterized in that: The workflow of the online product separation unit includes: achieving ion selective retention through electrodialysis under specific pH conditions, converting the calcium salt product into high-purity crystals through chemical conversion, and achieving catalyst recycling through regeneration treatment.

4. The production device for synthesizing succinic acid using carbon dioxide according to claim 1, characterized in that: The dynamic model of the artificial intelligence control center includes empirical parameters and real-time data fusion algorithm, which achieves precise control of CO2 concentration through periodic parameter adjustment to maintain the dynamic balance of the reaction system.

5. The production device for synthesizing succinic acid using carbon dioxide according to claim 1, characterized in that: The fermentation tank is equipped with an intelligent temperature control system and a pressure adaptive adjustment device. Through precise temperature control and dynamic pressure management, it maintains the high solubility of CO2 and ensures the stability of the reaction system.

6. The production device for synthesizing succinic acid using carbon dioxide according to claim 1, characterized in that: Specific genetically engineered strains are used for fermentation production, and the culture medium uses an optimized ratio of carbon source and nutrients, and efficient conversion is achieved by controlling the inoculation amount.

7. The production device for synthesizing succinic acid using carbon dioxide according to claim 1, characterized in that: An exhaust gas recycling module is set up to achieve efficient recycling of CO2 through gas purification and mixed reuse technology, significantly reducing raw material consumption.

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