Preparation for preventing and / or treating sugarcane albidosis and application thereof

Abstract

The invention relates to a preparation for preventing and / or treating sugarcane leukosis and application thereof, and relates to the technical field of biological agents. The preparation comprises bacillus mucilaginosus, bacillus megatherium, bacillus laterosporus, alternaria tenuissima activator protein, calcium acetate, magnesium sulfate and glucose. The preparation has a relatively good prevention and / or treatment effect on the sugarcane bacterial leaf streak, and also can promote the growth of the sugarcane at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of biological preparations, and in particular to a preparation for preventing and / or treating sugarcane white streak disease and its application. Background Art

[0002] Sugarcane white streak disease is a disease of sugarcane caused by the fungus Xanthomonas albicans. Infected sugarcane plants experience significant stunting, with thin stems and shortened internodes. Single or multiple white, creamy yellow, or light green stripes of varying widths may be observed on the leaves. As the disease progresses, sugarcane leaves gradually dry up from the tip to the edge until the entire leaf withers. Current research shows that sugarcane is susceptible to this disease when the soil is too wet or there is excessive rainfall. The disease is found in many sugarcane-growing countries and regions around the world. Existing measures for white streak disease generally focus on strengthening field management, timely tillage and loosening of the soil, and avoiding waterlogging in the fields. No effective pesticides have been found to effectively control it. Summary of the Invention

[0003] In view of the above problems, the present invention provides a preparation for preventing and / or treating sugarcane white streak disease. The preparation has a good preventive and / or therapeutic effect on sugarcane white streak disease and can also promote sugarcane growth.

[0004] The invention provides a preparation for preventing and / or treating sugarcane white streak disease, comprising: Bacillus gelatinosa, Bacillus megaterium, Bacillus laterosporus, Alternaria tenuis activating protein, calcium acetate, magnesium sulfate and glucose.

[0005] During their research on white streak disease, the inventors discovered that a combination of Bacillus gelatinosa, Bacillus megaterium, and Bacillus laterosus can inhibit the growth of Xanthomonas albostripe, and that Alternaria tenuissima activating protein can stimulate plant growth and increase chlorophyll content, thereby enhancing sugarcane's resistance to pathogens. Furthermore, calcium acetate and magnesium sulfate, under the action of glucose, can activate the enzyme system in sugarcane, promote plant growth and development, and help prevent cracking of sugarcane.

[0006] In one embodiment, the preparation includes the following raw materials at the following concentrations: Bacillus jelly (1-3) × 10 9 cfu / mL, Bacillus megaterium (1-3)×10 9 cfu / mL, Bacillus laterus (1-3) × 10 9 cfu / mL, Alternaria tenuis activation protein 20-40 mg / L, calcium acetate 15-20 mg / L, magnesium sulfate 10-13 mg / L, and glucose 10-15 mg / L.

[0007] In one embodiment, the preparation includes the following raw materials at the following concentrations: 2×10 9 cfu / mL, Bacillus megaterium 2×10 9 cfu / mL, Bacillus laterus 2×10 9 cfu / mL, Alternaria tenuis activation protein 30 mg / L, calcium acetate 18 mg / L, magnesium sulfate 12 mg / L, and glucose 13 mg / L.

[0008] The present invention also provides a pesticide for sugarcane, comprising an active ingredient and an adjuvant acceptable to the pesticide, wherein the active ingredient comprises the preparation.

[0009] In one embodiment, the excipient includes at least one of an excipient, a lubricant, an antioxidant, a preservative, a binder, a filler or a thickener.

[0010] In one embodiment, the pesticide is in the form of a powder, a wettable powder, a suspension, a dry suspension or a granule.

[0011] The present invention also provides use of the preparation in preparing pesticides for preventing and / or treating sugarcane white streak disease.

[0012] The present invention also provides a method for treating and / or preventing sugarcane white streak disease, which comprises applying the preparation or the pesticide to sugarcane.

[0013] In one embodiment, the interval between administrations is 40-80 days.

[0014] The present invention also provides use of the preparation or the pesticide in treating and / or preventing sugarcane white streak disease.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention provides a preparation for preventing and / or treating sugarcane white streak disease and its application. The preparation has good prevention and / or treatment effect on sugarcane white streak disease. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a picture of the early symptoms of white stripe disease;

[0018] Figure 2 This is a picture of the late stage infection symptoms of white stripe disease;

[0019] Figure 3 This is a schematic diagram of yellow-skinned sugarcane that has recovered after being cured of white stripe disease;

[0020] Figure 4 This is a schematic diagram of yellow-skinned sugarcane that has recovered after being cured of white stripe disease. DETAILED DESCRIPTION

[0021] To facilitate understanding of the present invention, the present invention will be described more fully below with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the accompanying drawings. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the present disclosure.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] source:

[0024] Unless otherwise specified, the reagents, materials, and equipment used in this example are all commercially available; and the experimental methods, unless otherwise specified, are all conventional experimental methods in the art.

[0025] Example 1

[0026] A preparation for preventing and / or treating sugarcane white streak disease.

[0027] The preparation method of the preparation comprises the following steps: mixing Bacillus gelatinosa, Bacillus megaterium, Bacillus laterus, Alternaria tenuis activation protein, calcium acetate, magnesium sulfate and glucose to obtain the preparation;

[0028] In this embodiment, the above preparation contains: 2×10 9 cfu / mL, Bacillus megaterium 2×10 9 cfu / mL, Bacillus laterus 2×10 9 cfu / mL, Alternaria tenuis activation protein 30 mg / L, calcium acetate 18 mg / L, magnesium sulfate 12 mg / L, and glucose 13 mg / L.

[0029] The prepared preparation is applied to yellow-skinned sugarcane once at an interval of about 80 days, or twice within 80 days to consolidate the effect.

[0030] Experimental example

[0031] 1. Experiments were conducted on yellow sugarcane using preparations with different formulas.

[0032] 1. Each formulation is basically the same as that in Example 1, except that different bacterial agents are used, as shown in the following table:

[0033] Table 1 Bacterial agent compounding of each formula

[0034] Bacillus gelatinosa Bacillus megaterium Bacillus laterus Bacillus subtilis Recipe A √ × √ × Recipe B × √ √ × Formulation C (Example 1) √ √ √ × Recipe D √ √ × √ Recipe E √ × × √ Recipe F × √ × √

[0035] Note: In the above table, “√” indicates that the formula uses this microbial agent, and “×” indicates that the formula does not use this microbial agent.

[0036] 2. Use different formulas of fungicides in Table 1 to apply pesticides to yellow-skinned sugarcane. In order to see the effect faster, choose to apply it in a short period of time with high concentration, apply it once every 10 days, and set CK as the blank control group, only water it. If it is infected with white stripe disease, apply 600-800 times the amount of carbendazim with the same dosage and application frequency as other groups of preparations. Plant it for one year, and collect statistics on the growth data and white stripe disease prevention and control data of yellow-skinned sugarcane.

[0037] (1) The growth data results are shown in Table 2.

[0038] Table 2 Statistics of growth characteristics of yellow-skinned sugarcane

[0039] deal with Height (cm) Diameter at breast height (cm) Weight (kg) Number of stem nodes CK 269.60±11.69c 3.52±0.17e 2.63±0.25c 24.50±2.17c A 302.10±7.60a 3.62±0.03d 2.86±0.25b 32.20±1.69a B 297.40±11.85ab 3.77±0.07c 3.43±0.26a 31.70±1.25ab C 289.30±9.26b 3.92±0.02b 3.06±0.11b 32.80±1.14a D 289.30±7.21b 4.12±0.10a 3.43±0.23a 31.70±1.25ab E 208.10±10.42e 3.42±0.18f 1.63±0.12e 22.50±0.53d F 250.40±22.76d 3.56±0.50de 2.34±0.40d 30.10±3.14b

[0040] The data in the table above show that the different microbial inoculants had a certain effect on the growth and development of yellow-skinned sugarcane. Treatments AD showed significantly better values ​​than the control (CK), with overall positive growth. Treatment EF showed a generally downward trend compared to the control (CK). Treatments A and B were dominant in height growth, reaching 302.10 cm and 297.40 cm, respectively. Treatment D was dominant in diameter at breast height (DBH), reaching 4.122 cm. Treatments B and D were dominant in weight growth, both reaching 3.43 kg. Treatments A, B, C, and D were dominant in node increase, with 32.2, 31.7, 32.8, and 31.7 nodes, respectively.

[0041] This suggests that a mixture of Bacillus laterus and other bacterial species is beneficial to the growth and development of yellow-skinned sugarcane. A mixture of Bacillus subtilis, Bacillus gelatinous, and Bacillus megaterium still favors the growth and development of yellow-skinned sugarcane, with some growth traits significantly improved. However, when mixed with Bacillus gelatinous and Bacillus megaterium, the growth of yellow-skinned sugarcane was inhibited. A comprehensive comparison of various growth values ​​shows that Treatment D exhibits better overall growth than the other treatments.

[0042] (2) The data on the control effect of white streak disease are shown in Table 3. The photos of yellow-skinned sugarcane white streak disease before and after treatment are shown in Table 3. Figures 1-4 As shown, Figure 1 This is a picture of the early symptoms of yellow-skinned sugarcane infected with white streak disease. Figure 2 This is a picture of the late symptoms of yellow-skinned sugarcane infected with white streak disease. Figure 3 This is a diagram of yellow-skinned sugarcane that has recovered after being cured of white stripe disease. Figure 4 This is a schematic diagram of yellow-skinned sugarcane that has recovered after being cured of white stripe disease.

[0043] Table 3 Statistics of white stripe disease prevention and control data

[0044]

[0045] Note: The definition of “duration of effect” in the above table is: the time from the disappearance of disease symptoms to the next appearance of the disease, calculated in days.

[0046] From the data in the above table, it can be seen that the effect of formula C on preventing and controlling white stripe disease is better than that of formula D. Considering that both of them have a promoting effect on the growth and development of yellow-skinned sugarcane, although formula C is slightly lower than formula D, considering the effect on preventing and controlling white stripe disease, the comprehensive effect of formula C is better than that of formula D.

[0047] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0048] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A preparation for preventing and / or treating sugarcane white streak disease, characterized in that: include: Bacillus gelatinosa, Bacillus megaterium, Bacillus laterus, Alternaria tenuis activator protein, calcium acetate, magnesium sulfate, and glucose.

2. The preparation according to claim 1, characterized in that The preparation includes the following raw materials at the following concentrations: Bacillus jelly (1-3) × 10 9 cfu / mL, Bacillus megaterium (1-3)×10 9 cfu / mL, Bacillus laterus (1-3) × 10 9 cfu / mL, Alternaria tenuis activation protein 20-40 mg / L, calcium acetate 15-20 mg / L, magnesium sulfate 10-13 mg / L, and glucose 10-15 mg / L.

3. The preparation according to claim 2, characterized in that The preparation includes the following raw materials at the following concentrations: 2×10 9 cfu / mL, Bacillus megaterium 2×10 9 cfu / mL, Bacillus laterus 2×10 9 cfu / mL, Alternaria tenuis activation protein 30 mg / L, calcium acetate 18 mg / L, magnesium sulfate 12 mg / L, and glucose 13 mg / L.

4. A pesticide for sugarcane, characterized in that: The invention comprises an active ingredient and an auxiliary material acceptable to pesticides, wherein the active ingredient comprises the preparation according to any one of claims 1 to 3.

5. The pesticide according to claim 4, characterized in that The auxiliary materials include at least one of excipients, lubricants, antioxidants, preservatives, adhesives, fillers or thickeners.

6. The pesticide according to claim 4, characterized in that The pesticide is in the form of a powder, a wettable powder, a suspension, a dry suspension or a granule.

7. Use of the preparation according to any one of claims 1 to 3 in the preparation of a pesticide for preventing and / or treating sugarcane white streak disease.

8. A method for treating and / or preventing sugarcane white streak disease, characterized in that: Sugarcane is sprayed with the formulation according to any one of claims 1 to 3 or the pesticide according to any one of claims 4 to 6.

9. The method according to claim 8, characterized in that The interval between the application of the pesticides is 40-80 days.

10. Use of the preparation according to any one of claims 1 to 3 or the pesticide according to any one of claims 4 to 6 in treating and / or preventing sugarcane white streak disease.

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