Freezing section immunohistochemical dyeing machine

Abstract

The invention discloses an immunohistochemical staining machine for frozen sections. The immunohistochemical staining machine comprises a shell assembly and a rack assembly, the reagent shelf is used for placing reagent bottles; the two dyeing frame assemblies are respectively positioned on two sides of the reagent frame and are used for realizing reagent flowing between the glass slide and the cover plate; the push-pull plate is inserted into the dyeing frame assembly and is used for placing a glass slide and a cover plate; the three-axis assembly is arranged above the dyeing frame assembly and is used for driving a liquid adding needle and a code scanner to move so as to dropwise add system liquid and scan bar codes on a glass slide and a reagent bottle; and the two needle washing pool assemblies correspond to the left-side three-axis assembly and the right-side three-axis assembly respectively and are used for washing the liquid adding needles. According to the device, intraoperative immunohistochemistry freezing, intraoperative HE freezing and special staining work can be carried out on frozen tissue at the same time, the operation process is simple, and the working efficiency is high.

Description

Technical Field

[0001] The invention belongs to the technical field of immunohistochemistry, and in particular relates to a frozen section immunohistochemical staining machine. Background Art

[0002] Intraoperative frozen-slide diagnosis uses rapid freezing to rapidly produce a pathological diagnosis during surgery, helping surgeons decide on the surgical procedure and extent. It is an urgent frontier in pathological diagnosis, crucially impacting the surgeon's surgical approach and outcomes, as well as the patient's treatment, recovery, and quality of life. Therefore, the requirements for slide preparation technology, quality, and pathologists are extremely high. This role is typically reserved for associate senior or senior attending physicians. However, due to the dual constraints of preparation technology and time, frozen-slide diagnosis during surgery is extremely challenging, placing both the slide preparation technician and the diagnostician under immense pressure and strain. Currently, some hospitals have implemented intraoperative frozen-slide immunohistochemical staining to aid diagnosis, enabling rapid staining of sections. Others utilize special stains to aid intraoperative frozen-slide diagnosis. However, due to time and personnel constraints, simultaneous immunohistochemistry and special staining of frozen tissues are nearly impossible. Furthermore, the complex and technically demanding procedures for intraoperative frozen-slide immunohistochemistry and special staining make it difficult to guarantee both quality and timeliness. Therefore, improvements to traditional immunohistochemistry equipment are necessary. Summary of the Invention

[0003] In order to solve the shortcomings of the existing technology, the purpose of the present invention is to provide a frozen section immunohistochemical staining machine, which can simultaneously perform intraoperative frozen immunohistochemistry, intraoperative frozen HE and special staining on frozen tissues, with simple operation process and high work efficiency.

[0004] In order to achieve the above objectives, the present invention adopts the following technical solutions: A frozen section immunohistochemical staining machine includes: a shell assembly, a frame assembly; a reagent rack for placing reagent bottles; two staining rack assemblies are provided, located on both sides of the reagent rack, for realizing the flow of reagents between the slide and the cover plate; a push-pull plate is inserted into the staining rack assembly, for placing the slide and the cover plate; a three-axis assembly is slidably arranged above the staining rack assembly, for driving the dosing needle and the barcode scanner to move so as to drip system liquid and scan the barcodes on the slide and the reagent bottle, the three-axis assembly including a left three-axis assembly and a right three-axis assembly corresponding to the two staining rack assemblies respectively and arranged in an interlaced manner in space; two needle washing pool assemblies are provided, corresponding to the left three-axis assembly and the right three-axis assembly respectively, for cleaning the dosing needle.

[0005] Preferably, the aforementioned dyeing rack assembly is fixed to the lower load-bearing plate by screws, and includes a bottom plate, hooks, a push-pull plate track and a cover plate; both sides of the push-pull plate track are fixedly connected to the lifting plate, and circular holes are provided at both ends of the front side, and guide columns matching the circular holes are provided on both sides of the top of the bottom plate. The guide columns are inserted into the circular holes, and the push-pull plate track can move up and down along the guide columns under the drive of the lifting plate; the liquid receiving plate is fixedly connected to the lower inner side of the lifting plate, and the top of the liquid receiving plate is slidably connected to the cover plate. The two sides of the cover plate are fixedly and vertically connected with hooks, and the lower part of the hooks is provided with a first guide port and a second guide port. ; A dyeing rack motor is fixedly arranged on the bottom plate, and the dyeing rack motor is connected to the driving shaft through transmission. Cams are fixedly connected on both sides of the driving shaft. A first cam follower matching the first guide port is fixed on the outer surface of the cam, and a rolling groove is provided on the inner surface. Under the rotation of the cam, the first cam follower moves back and forth along the first guide port to drive the cover plate to move forward and backward through the hook claw; a limiting guide column matching the second guide port and a second cam follower matching the rolling groove are provided at the bottom of the lifting plate. The second cam follower moves back and forth along the rolling groove to drive the lifting plate to move up and down.

[0006] Preferably, a roller support beam and a linear rail are fixedly provided at the middle position inside the aforementioned shell assembly. The three-axis assembly includes a three-axis motor, a Y-axis base plate, a cylindrical rack, an XY-axis connecting piece and a barcode scanner. The three-axis motor is fixed on the Y-axis base plate and is connected to the cylindrical rack for transmission. The lower end of the cylindrical rack is fixedly connected to the liquid detection module and the liquid adding needle. One side of the bottom end of the Y-axis base plate is fixedly connected to the Y-axis support piece, and the middle part of the bottom end is fixedly connected to the XY-axis connecting piece and the barcode scanner fixing plate on which the barcode scanner is installed. The bottom of the Y-axis support piece is movably connected to the roller support beam through a roller, and the bottom of the XY-axis connecting piece is slidably connected to the linear rail through a slider to drive the movement of the three-axis assembly.

[0007] Preferably, a photoelectric limit assembly is arranged between the aforementioned left three-axis assembly and the right three-axis assembly, and the photoelectric limit assembly includes a left optical coupler, a left shading plate, a three-axis shading plate, a right optical coupler, a right shading plate and a three-axis optical coupler. The left three-axis assembly moves to the left, and when the left optical coupler moves to the left shading plate, the left three-axis assembly slows down and stops moving; the right three-axis assembly moves to the right, and when the right optical coupler moves to the right shading plate, the right three-axis assembly slows down and stops moving; the left three-axis assembly and the right three-axis assembly move toward each other, and when the three-axis optical coupler moves to the three-axis shading plate, the two three-axis assemblies slow down and stop moving to prevent collision.

[0008] Preferably, the aforementioned needle washing pool assembly includes a reagent tube rack, a needle washing pool and a detection plate, and a slot for placing a mixing tube is provided on the top of the reagent tube rack.

[0009] Preferably, a refrigeration module is further included for maintaining a low temperature environment during the sample experiment. The refrigeration module is fixed to the load-bearing plate at the bottom of the rack assembly by screws and includes a cooling fan and a reagent rack detection plate for placing the reagent rack.

[0010] Preferably, a weighing assembly is also included for real-time monitoring of the liquid volume in the system liquid bottle, including a tray, a weighing sensor and a fixing frame.

[0011] Preferably, a pump valve assembly is further included to control the liquid system and distribute the system liquid to the two staining rack assemblies through the liquid dispenser.

[0012] Preferably, it also includes electrical components and heat dissipation components.

[0013] Preferably, the aforementioned housing assembly includes a housing, a touch screen and a magnetic touch screen pen.

[0014] The present invention is beneficial in that: (1) The present invention can simultaneously perform intraoperative frozen immunohistochemistry, intraoperative frozen HE and special staining on frozen tissues, with a simple operation process and high work efficiency; (2) The present invention is designed with dual sample loading arms, each equipped with a separate needle washing pool assembly. Through division of labor, cooperation and redundant design, the efficiency, accuracy and reliability of immunohistochemistry experiments are significantly optimized, and the experiment can be completed more quickly than the machines on the market, meeting the use requirements of the hospital. The reagent compartment is located between the two adjacent sample loading arms, sharing the reagent area. Through space intensification, process intelligence and hardware collaboration, the complexity and use cost of the equipment are significantly reduced while ensuring the efficiency and accuracy of the experiment. There is also a photoelectric limiter between the two liquid loading arms to avoid mutual interference. (3) The system liquid weighing component of the present invention can directly display the liquid volume, which is convenient for operators to intuitively understand the remaining amount of system liquid; the barcode scanner can quickly scan the slide information and reagent bottle information, and quickly and conveniently enter customer information; (4) The present invention can perform two-step needle washing, which makes the cleaning more thorough, avoids the problem of reagent contamination, and improves the staining quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a front view of the internal structure of the present invention; Figure 2 It is a rear view of the internal structure of the present invention; Figure 3 is a top view of the reagent rack of the present invention; Figure 4 It is a structural schematic diagram of the dyeing rack assembly of the present invention; Figure 5 is a perspective view of the dyeing rack assembly of the present invention; Figure 6It is a three-dimensional diagram of the needle washing pool assembly of the present invention; Figure 7 is a perspective view of the three-axis assembly of the present invention; Figure 8 It is a front view of the three-axis assembly of the present invention; Figure 9 is a front view of the housing assembly of the present invention; Figure 10 is a three-dimensional diagram of the refrigeration module of the present invention; Figure 11 This is a schematic diagram of the installation of the reagent bottle in the present invention; Figure 12 is a perspective view of the weighing assembly of the present invention; Figure 13 It is a structural diagram of the cam in the present invention; Figure 14 is a schematic diagram of the air path system of the present invention; Figure 15 It is a schematic diagram of the liquid path system in the present invention.

[0016] The meaning of the reference numerals in the figure: 1-housing assembly, 2-frame assembly, 201-load-bearing plate, 202-X-line rail fixing plate, 203-roller support beam, 3-three-axis assembly, 4-staining rack assembly, 5-refrigeration module, 6-system liquid bottle, 7-pump valve assembly, 8-weighing assembly, 9-electrical assembly, 10-reagent rack, 11-needle washing pool assembly, 12-mixing tube, 13-reagent bottle, 14-push-pull plate, 15-host, 16-heat dissipation assembly, 17-line rail, 18-slider, 19-synchronous belt , 20-synchronous wheel, 21-synchronous belt pressure plate, 101-housing, 102-touch screen, 103-magnetic touch screen pen, 201-load-bearing plate, 202-X axis rail fixing plate, 301-cylindrical rack, 302-ball spline, 303-ball spline shaft, 304-liquid detection module, 305-liquid addition needle, 306-barcode scanner fixing plate, 307-barcode scanner, 308-Y axis bottom plate, 309-Y axis support, 310-roller, 311-XY axis connector, 312-left shading sheet , 313-left optical coupler, 314-right shading sheet, 315-right optical coupler, 316-three-axis shading sheet, 317-three-axis optical coupler, 401-turbine, 402-worm, 403-cooling fan, 404-drive shaft, 405-cam, 4051-first cam follower, 4052-rolling notch, 406-three-axis motor, 407-hook claw, 4071-first guide port, 4072-second guide port, 408-temperature control component, 409-push-pull plate detection optical coupler, 410- Sliding plate track, 411-pressure cover, 412-staining rack slide rail, 413-staining rack slider, 414, slider fixing seat, 415-liquid receiving plate, 416-limiting guide column, 417-lifting plate, 420-cover plate, 421-bottom plate, 422-guide column, 501-cooling fan, 502-reagent rack detection plate, 601-filter, 801-tray, 802-weighing sensor, 803-fixed frame, 1101-reagent tube rack, 1102-needle washing pool, 1103-detection plate. DETAILED DESCRIPTION

[0017] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] See also Figures 1 and 2, a frozen section immunohistochemistry staining machine includes: a shell assembly, a frame assembly 2; a reagent rack 10 for placing reagent bottles 13; a staining rack assembly 4, which is provided with two, respectively located on both sides of the reagent rack 10, for realizing the flow of reagents between the slide and the cover plate; a push-pull plate 14, inserted into the staining rack assembly 4, for placing the slide and the cover plate; a three-axis assembly 3, arranged above the staining rack assembly 4, for driving the liquid adding needle 305 and the barcode scanner 307 to move, so as to drip system liquid and scan the barcodes on the slide and the reagent bottle 13, the three-axis assembly 3 includes a left three-axis assembly 3 and a right three-axis assembly 3 corresponding to the two staining rack assemblies 4 respectively and arranged in space staggered, a photoelectric limit assembly is provided between the left three-axis assembly 3 and the right three-axis assembly 3, for preventing the two three-axis assemblies 3 from colliding; a needle washing pool 1102 assembly 11 is provided with two, respectively corresponding to the left three-axis assembly 3 and the right three-axis assembly 3, for cleaning the liquid adding needle 305.

[0019] See also Figures 3-5 The dyeing rack assembly 4 is fixed to the lower load-bearing plate 201 by screws, and includes a worm gear, a worm 402, a cooling fan 403, a drive shaft 404, a dyeing rack motor, a hook 407, a temperature control component 408, a push-pull plate detection optical coupler 409, a push-pull plate track 410, a pressure cover 411, a bottom plate 419, a cover plate 420 and a guide column 422. Both sides of the push-pull plate track 410 are fixedly connected to the lifting plate 417, and circular holes are provided at both ends of the front side. Guide columns 422 matching the circular holes are provided on both sides of the top of the bottom plate 419. The guide columns 422 are inserted into the circular holes, and the push-pull plate track can move up and down along the guide columns 422 under the drive of the lifting plate 417. The liquid receiving plate 415 is fixedly connected to the lower inner side of the lifting plate. The bottom of the cover plate 420 is fixed with a slide rail 412 via screws. The liquid receiving plate 415 is fixed with a slider fixing seat 414. The slider fixing seat 414 is slidably connected to the slide rail 412 via a slider 413, thereby achieving a sliding connection between the liquid receiving plate 415 and the cover plate, thereby enabling the hook to drive the cover plate to move back and forth on the liquid receiving plate. Hooks are fixedly connected to both sides of the cover plate in a vertical position. The lower portion of the hook is provided with a first guide opening and a second guide opening.

[0020] A dyeing rack motor is fixedly mounted on the bottom plate 419. The dyeing rack motor is connected to a worm gear through a worm 402, and the worm gear is connected to a drive shaft 404. Cams 405 are fixedly connected to both sides of the drive shaft. A first cam follower 4051 matching the first guide opening is fixed on the outer surface of the cam 405, and a rolling groove 4052 is provided on the inner surface. Under the rotation of the cam 405, the first cam follower 4051 moves back and forth along the first guide opening to drive the cover plate to move forward and backward through the hook 407; a limiting guide column matching the second guide opening and a second cam follower matching the rolling groove 4052 are provided at the bottom of the lifting plate. The second cam follower moves back and forth along the rolling groove 4052 to drive the lifting plate to move up and down.

[0021] When push-pull plate 14 is inserted into the dyeing rack, the push-pull plate detection optocoupler 409 senses the movement and feeds the signal back to the computer 15. The dyeing rack is driven by the dyeing rack motor, which rotates the worm 402. The worm 402 rotates the worm gear, and the drive shaft 404 rotates with the worm gear. The drive shaft 404 then drives the cam 405 to move. The synchronous movement of the cams 405 on both sides pulls the hook 407.

[0022] The rolling notch of the cam 405 is as follows Figure 13 As shown, when the second cam follower rotates from point A to point B along the rolling groove, it drives the lifting plate to move downward, thereby driving the push-pull plate track 410 to move downward, and the pressure cover 411 will press on the cover, allowing the reagent to flow better between the cover and the slide; when the cam 405 continues to rotate, and the second cam follower rotates from point B to point C along the rolling groove, the first cam follower enters the first guide port, moves upward along the first guide port, and the hook 407 moves horizontally, and then the hook 407 pulls the cover out horizontally, and the slide can be exposed to the air; when the cam 405 continues to rotate, and the second cam follower rotates from point C to point D along the rolling groove, the second cam follower drives the lifting plate to move upward, thereby driving the push-pull plate track 410 to move upward, and the pressure cover 411 will not press on the cover, releasing the pressure. This form of movement can more accurately control the movement of the staining rack to meet experimental requirements.

[0023] Figure 13 The names and status of each point are as follows: Point A: starting point; Point B: working station; Point C: starting point for recovery; Point D: Position No. 2; Point E: mechanical limit point; Regarding the position of the cover: Section B→C: Position 0 (cover position) is adjustable; Section D→E: Position 2 (cover position) is adjustable; Regarding the vertical movement of the dyeing rack: Section A→B: descending from the starting point to the working station; Section B→C: no rise and fall, constant lowest point, unchanged; Section C→D: rising to the second lowest point; Section D→E: no rise and fall, constant second lowest point, unchanged.

[0024] In addition, there is a separate temperature control system in the staining rack. During the frozen section immunohistochemistry experiment, the temperature requirements are relatively strict. The reliability of antigen repair can be achieved through the temperature control component 408 to meet the requirements of the experiment.

[0025] See also Figure 6 The needle washing pool assembly 11 includes a reagent tube rack 1101, a needle washing pool 1102 and a detection plate 1103. The top of the reagent tube rack 1101 is provided with a slot for placing the mixing tube 12. When the operator places the mixing tube 12 into the slot of the reagent tube rack 1101, the detection plate 1103 will sense the presence of the mixing tube 12 and feed the information back to the computer host 15. The operator can judge whether the mixing tube 12 is placed through the operation interface. Of course, if the mixing tube 12 is not placed, the machine will automatically prompt to place the mixing tube 12, otherwise the experiment cannot be carried out. When doing the experiment, the adding needle 305 needs to be cleaned. At this time, the robotic arm will take the adding needle 305 to the top of the needle washing pool 1102 to clean the adding needle 305.

[0026] See also Figures 7 and 8 A roller support beam and a linear rail are fixedly installed in the middle position inside the housing assembly. The three-axis assembly includes a three-axis motor, a Y-axis base plate, a cylindrical rack, an XY-axis connector, and a barcode scanner. The three-axis motor is fixed to the Y-axis base plate and is connected to the cylindrical rack for transmission. The lower end of the cylindrical rack is fixedly connected to the liquid detection module and the liquid addition needle. One side of the bottom end of the Y-axis base plate is fixedly connected to the Y-axis support member, and the middle of the bottom end is fixedly connected to the XY-axis connector and the barcode scanner fixing plate with the barcode scanner installed. The bottom of the Y-axis support member is movably connected to the roller support beam through a roller, and the bottom of the XY-axis connector is slidably connected to the linear rail through a slider to drive the movement of the three-axis assembly. The synchronous belt pressure plate 21 fixes the synchronous belt 19 to the XY-axis connector 311 with screws. The motor drives the synchronous wheel to rotate, and the synchronous wheel moves the XY-axis connector 311 through the synchronous belt, thereby realizing the movement of the three-axis assembly in the X-axis direction.

[0027] The three-axis motor 406 drives the ball spline 302 shaft to rotate, and the ball spline 302 shaft drives the ball spline 302 to rotate, so as to drive the cylindrical rack 301 to move up and down, thereby driving the up and down movement of the dosing needle 305. The dosing needle 305 is treated with Teflon, which reduces the resistance and makes the liquid flow smoother. This method makes the movement smoother and more accurate, and the addition of reagents is more precise, which protects the experiment. Before the experiment begins, the three-axis assembly 3 moves with the code scanner 307. The code scanner 307 automatically recognizes the two-dimensional code of the slide and reagent bottle 13, extracts the slide and reagent information, and the host 15 automatically calculates the amount of reagent required for this experiment based on the scanned slide information. In addition, the dosing needle 305 can also detect the depth of the reagent liquid level through the liquid detection module 304 when taking liquid to calculate the remaining amount of reagent. If the reagent is insufficient before the experiment begins, the device will automatically prompt to prevent the failure of the experiment. The liquid volume is detected by the liquid detection module 304 and then displayed, so that the experimenter can intuitively see the remaining amount of liquid and prevent the failure of the experiment due to insufficient liquid in the reagent bottle 13.

[0028] See also Figure 2 A photoelectric limit assembly is provided between the left three-axis assembly and the right three-axis assembly to prevent the two three-axis assemblies from colliding. The photoelectric limit assembly includes a left optical coupler 313, a left light shielding plate 312 and a three-axis light shielding plate 316. The right three-axis assembly 3 also includes a right optical coupler 315, a right light shielding plate 314 and a three-axis optical coupler 317. The left three-axis assembly 3 moves to the left. When the left optical coupler 313 moves to the left light shielding plate 312, the left three-axis assembly 3 slows down and stops moving; the right three-axis assembly 3 moves to the right. When the right optical coupler 315 moves to the right light shielding plate 314, the right three-axis assembly 3 slows down and stops moving; the left three-axis assembly 3 and the right three-axis assembly 3 move toward each other. When the three-axis optical coupler 317 moves to the three-axis light shielding plate 316, the two three-axis assemblies 3 slow down and stop moving to prevent collision.

[0029] See also Figure 9 The housing assembly includes a housing 101, a touch screen 102, and a magnetic stylus 103. Commercially available immunohistochemistry machines are designed with separate computer hosts 15 and display screens, which significantly occupies space in hospitals. However, the present invention integrates the computer host 15 and display screen into the machine body, significantly reducing space. Furthermore, the computer host 15 can be operated with a stylus, making it more convenient for operators.

[0030] The machine's internal structure is remarkably compact, with dual dosing arms arranged in a staggered arrangement. The two dosing arms share a common reagent area, significantly reducing footprint. Because the needle wash basin 1102 is frequently used during experiments, each dosing arm has its own dedicated basin, eliminating interference and reducing experiment time. A photoelectric stopper ensures flexibility between the two dosing arms, preventing interference.

[0031] See also Figures 10-11 The immunohistochemical stainer also includes a refrigeration module 5, which is used to maintain a low temperature during sample testing. The refrigeration module 5 is screwed to the lower load-bearing plate 201 and includes a cooling fan 501 and a reagent rack detection plate 502. The reagent rack 10 is plugged into the reagent rack detection plate 502. Reagent bottles 13 can be placed on the reagent rack 10, and then the refrigeration module 5 is inserted. The cooling fan 501 removes heat. The refrigeration module 5 in the immunohistochemical instrument is primarily used to maintain a low temperature during the experiment to ensure sample stability and reagent activity. This refrigeration module 5 utilizes semiconductor refrigeration technology, which exploits the Peltier effect. This semiconductor refrigeration utilizes the Peltier effect, whereby when current passes through two different semiconductor materials, heat is absorbed or released at the junction. This heat is then removed through heat conduction and accelerated by the cooling fan 403. The reagent rack detection plate 502 detects whether the reagent rack 10 is properly placed and then feeds the data back to the computer host 15. The refrigeration module 5 provides reagent refrigeration, maintaining reagent stability. This not only maintains optimal reagent performance during the experiment but also prevents volatilization and reduces unnecessary waste gas generation.

[0032] See also Figure 12 The immunohistochemical staining machine weighing assembly 8 is used to monitor the liquid volume in the system liquid bottle 6 in real time, and includes a tray 801, a weighing sensor 802, and a fixing frame 803. The system liquid bottle 6 containing the auxiliary reagent is placed on the tray 801. The weighing sensor 802 will feed back the data to the computer host 15, and the volume of the auxiliary reagent can be known on the operation interface. Before doing the experiment, the value displayed on the operation interface can be used to know whether the capacity of the auxiliary reagent meets the experimental requirements. If the liquid volume calculated by the computer host 15 is insufficient, when the program is started, the operation interface will indicate that there is a lack of liquid, prompting the operator to add the auxiliary reagent again to prevent the experiment from failing.

[0033] The immunohistochemical staining machine also includes a pump valve assembly 7, which is used to control the liquid system and distribute the system liquid to the two staining rack assemblies 4 through a liquid dispenser; it also includes an electrical assembly 9, which is fixed to the rack assembly 2 by screws; the immunohistochemical staining machine also includes a heat dissipation assembly 16, which is fixed to the X-ray rail 17 fixing plate by screws.

[0034] See also Figure 14The immunohistochemical staining machine also includes an air duct system. Air enters from both sides of the machine, moves upward, and is discharged from the back of the machine. This air duct system can provide good heat dissipation for the machine, allowing it to operate stably and efficiently, and escorting the experiment.

[0035] See also Figure 15 The immunohistochemical staining machine also includes a liquid circuit system, in which the system liquid of the machine enters two injection pumps through a liquid separator. The two injection pumps add liquid to the two staining racks respectively. There is a filter 601 on the system liquid bottle 6 on the machine, which can filter impurities in the liquid, thereby protecting the liquid circuit system from being blocked. The two staining racks each have a valve and share a diaphragm pump. When there is a toxic liquid such as DAB in the liquid, the liquid circuit system will discharge the liquid in the pipeline into the toxic waste liquid barrel. When there is no contaminated liquid in the pipeline, the liquid circuit system will discharge the liquid into the non-toxic waste liquid barrel. The liquid in the cold storage module and the needle washing pool 1102 are respectively controlled by a valve and share a pump, which is controlled by the liquid circuit system to discharge the liquid into the toxic waste liquid barrel or the non-toxic waste liquid barrel.

[0036] In order to better illustrate the present invention, its working process is specifically described below: First, the tissue is frozen and sliced, fixed, dehydrated, transparent, embedded, and sliced, and then the staining experiment is performed. The experimental steps are as follows: (1) Fill the system liquid bottle 6 with system liquid; (2) Insert the reagent bottles 13 to be used for the experiment into the push-pull plate 14. The liquid in the reagent rack 10 is the reagent (primary antibody, secondary antibody, antibody diluent, DAB reagent, hematoxylin reagent, etc.), and then insert the reagent rack 10 into the refrigeration module 5; (3) Place the mixing tube 12 into the slot of the needle washing pool assembly 11; (4) Place 6 slides and a cover plate on the push-pull plate 14, attach labels to the slides, and then insert the push-pull plate 14 into the staining rack assembly 4; (5) The weighing component 8 will display the weight of the system liquid bottle 6; (6) The three-axis assembly 3 will carry the barcode scanner 307 to scan the area where the staining rack assembly 4 and the reagent rack 10 are located, and scan the barcodes on the slides and reagent bottles 13; (7) The liquid volume is detected by the liquid detection module 304 and displayed.

[0037] During the experiment: the frozen section immunohistochemical staining machine is started. Each part of the present invention automatically runs according to the predetermined experimental process under the control of the software. The specific operation process is as follows: (1) The pump-valve assembly 7 delivers the system liquid in the system liquid bottle 6 to the liquid adding needle 305 through the pipeline. The three-axis assembly 3 moves the liquid adding needle 305 to the position required for the experiment and drips the system liquid; (2) When the experimental temperature does not meet the requirements, the machine will automatically adjust. If the experimental temperature is low, but the experimental temperature is relatively high, the heating block on the staining rack assembly 4 will automatically heat up and meet the experimental requirements in a very short time. If the experimental temperature is high, but the experimental temperature is relatively low, the heat dissipation assembly 16 will automatically turn on and meet the experimental requirements in a very short time. (3) After the addition of the reagent, the dosing needle 305 goes to the needle washing pool assembly 11 for two-step needle washing, which includes washing the inner wall and outer wall of the dosing needle 305; (4) After the slide is completely stained, the operator removes the slide.

[0038] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the above embodiments do not limit the present invention in any form, and any technical solutions obtained by equivalent replacement or equivalent transformation fall within the scope of protection of the present invention.

Claims

1. A frozen section immunohistochemical staining machine, characterized in that: Including: shell assembly, rack assembly; Reagent rack, used to place reagent bottles; There are two staining rack assemblies, one on each side of the reagent rack, used to achieve the flow of reagents between the slide and the cover plate; The push-pull plate is inserted into the staining rack assembly and is used to place slides and cover plates; A three-axis assembly is slidably arranged above the staining rack assembly and is used to drive the dosing needle and barcode scanner to add system fluid and scan barcodes on slides and reagent bottles. The three-axis assembly includes a left three-axis assembly and a right three-axis assembly that correspond to the two staining rack assemblies and are staggered in space. There are two needle washing pool assemblies, corresponding to the left three-axis assembly and the right three-axis assembly respectively, for cleaning the liquid adding needle.

2. A frozen section immunohistochemical staining machine according to claim 1, characterized in that: The dyeing rack assembly is fixed to the lower load-bearing plate by screws, and includes a bottom plate, hooks, a push-pull plate track and a cover plate; Both sides of the push-pull plate track are fixedly connected to the lifting plate, and circular holes are provided at both ends of the front side. Guide columns matching the circular holes are provided on both sides of the top of the bottom plate. The guide columns are inserted into the circular holes, and the push-pull plate track can move up and down along the guide columns under the drive of the lifting plate; The liquid receiving plate is fixedly connected to the inner side of the lower part of the lifting plate, the top of the liquid receiving plate is slidably connected to the cover plate, both sides of the cover plate are fixedly and vertically connected with hooks, and the lower part of the hook is provided with a first guide port and a second guide port; The bottom plate is fixedly provided with a dyeing frame motor, which is transmission-connected to a driving shaft. Cams are fixedly connected to both sides of the driving shaft. A first cam follower matching the first guide opening is fixed on the outer surface of the cam, and a rolling groove is provided on the inner surface. Under the rotation of the cam, the first cam follower moves back and forth along the first guide opening, thereby driving the cover plate to move forward and backward through the hook claw. The bottom of the lifting plate is provided with a limiting guide column matching the second guide opening and a second cam follower matching the rolling groove. The second cam follower moves back and forth along the rolling groove to drive the lifting plate to move up and down.

3. A frozen section immunohistochemical staining machine according to claim 1, characterized in that: A roller support beam and a linear rail are fixedly arranged at the middle position inside the shell assembly. The three-axis assembly includes a three-axis motor, a Y-axis base plate, a cylindrical rack, an XY-axis connecting piece and a code scanner. The three-axis motor is fixed to the Y-axis base plate and is transmission-connected to the cylindrical rack. The lower end of the cylindrical rack is fixedly connected to the liquid detection module and the liquid adding needle. One side of the bottom end of the Y-axis base plate is fixedly connected to the Y-axis support piece, and the middle part of the bottom end is fixedly connected to the XY-axis connecting piece and the code scanner fixing plate on which the code scanner is installed. The bottom of the Y-axis support piece is movably connected to the roller support beam through a roller, and the bottom of the XY-axis connecting piece is slidably connected to the linear rail through a slider to drive the movement of the three-axis assembly.

4. A frozen section immunohistochemical staining machine according to claim 3, characterized in that: A photoelectric limit assembly is arranged between the left three-axis assembly and the right three-axis assembly, and the photoelectric limit assembly includes a left optical coupler, a left shading plate, a three-axis shading plate, a right optical coupler, a right shading plate and a three-axis optical coupler. The left three-axis assembly moves to the left, and when the left optical coupler moves to the left shading plate, the left three-axis assembly slows down and stops moving; the right three-axis assembly moves to the right, and when the right optical coupler moves to the right shading plate, the right three-axis assembly slows down and stops moving; the left three-axis assembly and the right three-axis assembly move toward each other, and when the three-axis optical coupler moves to the three-axis shading plate, the two three-axis assemblies slow down and stop moving to prevent collision.

5. The frozen section immunohistochemical staining machine according to claim 1, characterized in that: The needle washing pool assembly includes a reagent tube rack, a needle washing pool and a detection plate. The top of the reagent tube rack is provided with a slot for placing a mixing tube.

6. A frozen section immunohistochemical staining machine according to claim 1, characterized in that: It also includes a refrigeration module for maintaining a low-temperature environment during the sample experiment. The refrigeration module is fixed to the load-bearing plate at the bottom of the rack assembly by screws, and includes a cooling fan and a reagent rack detection plate for placing the reagent rack.

7. The frozen section immunohistochemical staining machine according to claim 1, characterized in that: Also included is a weighing assembly for real-time monitoring of the liquid level in the system's liquid bottle, including a tray, a weighing sensor, and a fixing bracket.

8. The frozen section immunohistochemical staining machine according to claim 1, characterized in that: It also includes a pump valve assembly for controlling the liquid system and distributing the system liquid to the two staining rack assemblies through the dispenser.

9. The frozen section immunohistochemical staining machine according to claim 1, characterized in that: Also includes electrical components and heat dissipation components.

10. The frozen section immunohistochemical staining machine according to claim 1, characterized in that: The housing assembly includes a housing, a touch screen and a magnetic touch screen pen.

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