Improved soil culture device and organic carbon mineralization analysis method thereof

Abstract

The invention relates to the technical field of agricultural science, in particular to an improved soil culture device and an organic carbon mineralization analysis method thereof.The improved soil culture device comprises an air compressor used for providing a stable air source in the experiment process; the air supply valve comprises a valve body, an inlet of the valve body is communicated with an outlet of the air compressor, and a plurality of independently arranged air supply pipes are arranged on the valve body; the culture bottle is provided with an air inlet head and an air outlet head which are independently arranged, and the air inlet head and the air outlet head are respectively communicated with an inner cavity of the culture bottle; and the air inlet head is detachably connected with the air supply pipe. The device is simple in structure, convenient to use and low in test cost, test data in the test process can be accurately controlled, the test repeatability and reliability are improved, the accuracy of soil organic carbon mineralization analysis is improved, and the device is suitable for long-term or large-scale organic carbon mineralization experiments.

Description

Technical Field

[0001] The present invention relates to the field of agricultural science and technology, and in particular to an improved soil cultivation device and an organic carbon mineralization analysis method thereof. Background Art

[0002] Soil organic carbon (SOC) mineralization, that is, the process of converting organic carbon into inorganic carbon, is a core link in the global carbon cycle. Soil organic carbon mineralization is the largest source of CO2 emissions in terrestrial ecosystems and plays a vital role in regulating atmospheric CO2 concentration and the process of global warming.

[0003] Conventional methods for analyzing soil organic carbon mineralization currently use gas cylinders as a gas supply source, coupled with a culture device to control gas exchange. While this traditional method of supplying gas via gas cylinders can meet certain experimental needs, it presents issues such as unstable gas supply and difficulty controlling gas flow. Existing technologies, such as patents CN10512345A and CN10498567A, both utilize gas cylinders and fixed flow control devices to achieve soil culture and gas analysis. However, these methods are limited in gas management and control accuracy, making it difficult to maintain relatively precise and stable conditions during long-term culture experiments.

[0004] Therefore, the present invention designs an improved soil culture device and an organic carbon mineralization analysis method thereof to solve the above technical problems. Summary of the Invention

[0005] The purpose of the present invention is to provide an improved soil cultivation device and an organic carbon mineralization analysis method thereof to solve the problems existing in the prior art.

[0006] To achieve the above objectives, the present invention provides the following solutions: The present invention provides an improved soil culture device, comprising:

[0007] An air compressor, which is used to provide a stable air source during the experiment;

[0008] An air supply valve, the air supply valve comprising a valve body, the inlet of the valve body being connected to the outlet of the air compressor, and the valve body being provided with a plurality of independently arranged air supply pipes;

[0009] A culture bottle is provided with an independently arranged air inlet head and an air outlet head, the air inlet head and the air outlet head are respectively communicated with the inner cavity of the culture bottle; the air inlet head is detachably connected to the air supply pipe.

[0010] Preferably, the culture bottle comprises a transparent bottle body, the bottle body is provided with a detachable bottle stopper, and the air inlet head and the air outlet head are respectively plugged into the bottle stopper.

[0011] Preferably, the air inlet head is plugged into the bottle stopper at an angle, and the air outlet head is plugged into the bottle stopper vertically, and the inner diameter of the air inlet head is smaller than the inner diameter of the air outlet head.

[0012] Preferably, an air inlet pipe is provided between the air outlet of the air compressor and the valve body, the air inlet pipe is connected to a pressure gauge, and the pressure gauge is electrically connected to a control box provided on the air compressor.

[0013] Preferably, the air compressor includes a gas cylinder, the air inlet pipe is arranged between the gas cylinder and the valve body, the inlet of the gas cylinder is connected to the compressor, and the compressor is electrically connected to the control box.

[0014] The present invention also discloses an organic mineralization analysis method based on the improved soil culture device, comprising the following steps:

[0015] Add fresh soil and sterile water to the culture bottle and adjust the humidity to the required level for the experiment;

[0016] The culture bottles filled with soil are sealed with sealing film and cultured under a set environment to stabilize the activity of soil microorganisms;

[0017] The culture bottles after pre-culture continue to be cultured, and CO2 gas is collected regularly during the culture process. The moisture content of the culture soil is kept constant during the culture process.

[0018] The air inlet and outlet are connected to the outside world to maintain ventilation and stabilize soil respiration;

[0019] Ventilate the culture bottle so that the air provided by the air compressor replaces the gas in the culture bottle;

[0020] Keep the air inlet and outlet heads closed to the outside world respectively, continue to cultivate the soil in the culture bottle, then mix the gas in the culture bottle evenly and extract the gas for measurement.

[0021] Preferably, the soil moisture content needs to be tested and the soil field water holding capacity needs to be determined before the test to obtain soil data to facilitate subsequent testing.

[0022] Preferably, the culture flask ventilation comprises the following steps:

[0023] Start the air compressor and introduce air into the culture bottle through the air inlet head;

[0024] Open the air outlet and adjust the air outlet speed so that the air inlet and outlet speeds of the culture bottle are the same;

[0025] The culture bottle is continuously ventilated to exhaust the air inside the culture bottle;

[0026] First close the air outlet of the culture bottle, and then close the air inlet of the culture bottle to keep the culture bottle in a closed state.

[0027] Preferably, after closing the gas inlet of the culture bottle, a sample is taken through the gas outlet to measure the CO2 content of the gas.

[0028] Preferably, the step of uniformly mixing the gas in the culture bottle comprises:

[0029] Connect the syringe to the gas outlet;

[0030] Open the outlet valve on the outlet head and pull in and out the syringe several times to mix the gas in the culture bottle evenly; close the outlet valve and then remove the syringe.

[0031] Compared with the prior art, the present invention has the following advantages and technical effects: the present invention discloses an improved soil culture device, in which an air compressor serves as a gas supply source to replace traditional gas cylinders, and can provide continuous and stable gas flow through its own regulating mechanism, avoiding the flow fluctuation problem caused by pressure changes in traditional gas cylinders, providing stable air for the entire experimental process, being the power core of the device, and having lower cost. There is no need to reserve zero gas, and there is no need to frequently replace gas cylinders, which reduces the frequency of experimental interruptions and the complexity of operation, and allows experiments to be carried out at any time, and also avoids the storage pressure of zero gas storage; at the same time, the air compressor is reusable, reducing dependence on disposable gas cylinders, and reducing the cost of gas storage, transportation and replacement; at the same time, it avoids the environmental risks after the gas cylinders are discarded, and conforms to the concept of environmental protection; the gas supply valve comprises a valve body and several independent gas supply pipes, the inlet of the valve body is connected to the outlet of the air compressor, and the gas supply pipe serves as a branch channel to realize independent gas supply control for multiple culture bottles, which can facilitate large-scale experiments and improve the efficiency of experiments. At the same time, batch experiments improve the accuracy of the experiments, and can The device ensures that the air intake conditions of different culture bottles are consistent, reducing experimental errors. The air intake parameters can also be flexibly adjusted for different culture bottles to meet the needs of multiple groups of control experiments, broadening the applicable scenarios of the device. As a container for soil culture, the culture bottle is equipped with an independent air inlet head and air outlet head, both of which are connected to the inner cavity of the bottle. The air inlet head and the air supply pipe are detachably connected to ensure that the gas can enter the culture bottle in a directional manner. At the same time, gas exchange or collection is achieved through the air outlet head, which makes it convenient to remove the culture bottle separately for gas collection, sample processing and other operations, enhancing flexibility. Specifically, the stable air generated by the air compressor is distributed to each air supply pipe through the valve body, enters the culture bottle through the air inlet head, exchanges gas with the soil in the bottle, and finally completes gas circulation or collection through the air outlet head. The detachable connection between the air inlet head and the valve body facilitates the installation, replacement and separate operation of the culture bottle. The stable gas environment and precise flow control ensure the consistency of CO2 production and exchange during soil organic carbon mineralization, making long-term culture data more reproducible and comparable, and providing a more reliable experimental basis for organic carbon mineralization analysis.

[0032] The present invention has a simple structure, is easy to use, and has low test cost. It can accurately control the test data during the test process, improve the repeatability and reliability of the test, and improve the accuracy of soil organic carbon mineralization analysis. It is suitable for long-term or large-scale organic carbon mineralization experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0034] Figure 1 This is a schematic structural diagram of the improved soil culture device of the present invention;

[0035] Figure 2 This is a schematic diagram of the structure of the culture bottle of the present invention;

[0036] In the figure: 1. Air compressor; 2. Air supply valve; 3. Culture bottle; 11. Air inlet pipe; 12. Pressure gauge; 13. Main control valve; 14. Gas storage bottle; 15. Compressor; 16. Control box; 17. Heat dissipation module; 21. Valve body; 22. Air supply pipe; 23. Air supply valve; 24. Branch air pipe; 31. Bottle body; 32. Bottle stopper; 33. Air inlet head; 34. Air outlet head; 35. Air inlet valve; 36. Air outlet valve. DETAILED DESCRIPTION

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0038] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0039] Reference Figures 1 to 2 As shown, this embodiment provides an improved soil cultivation device, comprising:

[0040] Air compressor 1, which is used to provide a stable air source during the experiment;

[0041] The air supply valve 2 includes a valve body 21, the inlet of the valve body 21 is connected to the outlet of the air compressor 1, and a plurality of independently arranged air supply pipes 22 are provided on the valve body 21;

[0042] The culture bottle 3 is provided with an independently arranged air inlet head 33 and an air outlet head 34 , which are respectively communicated with the inner cavity of the culture bottle 3 ; the air inlet head 33 is detachably connected to the air supply pipe 22 .

[0043] The present invention discloses an improved soil culture device, which mainly consists of three parts: an air compressor 1, an air supply valve 2 and a culture bottle 3. The air compressor 1 serves as a gas supply source and replaces the traditional gas cylinder. It can provide a continuous and stable gas flow through its own control mechanism, avoiding the flow fluctuation problem caused by pressure changes in the traditional gas cylinder, providing stable air for the entire experimental process, and is the power core of the device. It is more cost-effective, does not require pre-set zero gas, and does not require frequent replacement of gas cylinders, which reduces the frequency of experimental interruptions and the complexity of operation. Experiments can be carried out at any time and the pressure of zero gas storage is avoided. At the same time, the air compressor 1 is reusable, reducing dependence on disposable gas cylinders and reducing the cost of gas storage, transportation and replacement. At the same time, it avoids the environmental risks after the gas cylinder is discarded, which is in line with the concept of environmental protection. The air supply valve 2 includes a valve body 21 and a plurality of independent air supply pipes 22. The inlet of the valve body 21 is connected to the outlet of the air compressor 1. The air supply pipe 22 serves as a branch channel to realize independent air supply control of multiple culture bottles 3, which can facilitate large-scale experiments and improve experimental efficiency. At the same time, batch experiments improve the accuracy of the experiments and can ensure the accuracy of different culture bottles 3. The air intake conditions are consistent, which reduces experimental errors. The air intake parameters can also be flexibly adjusted for different culture bottles 3 to meet the needs of multiple groups of control experiments, broadening the applicable scenarios of the device; the culture bottle 3 is used as a container for soil culture, and is provided with an independent air inlet head 33 and an air outlet head 34, both of which are connected to the inner cavity of the bottle; the air inlet head 33 is detachably connected to the air supply pipe 22 to ensure that the gas can enter the culture bottle 3 in a directional manner, and at the same time, gas exchange or collection is achieved through the air outlet head 34, which is convenient for removing the culture bottle 3 separately for gas collection, sample processing and other operations, thereby enhancing flexibility; specifically, the air compressor The stable air generated by the valve body 21 is distributed to each air supply pipe 22, enters the culture bottle 3 through the air inlet head 33, exchanges gas with the soil in the bottle, and finally completes the gas circulation or collection through the air outlet head 34; the detachable connection between the air inlet head 33 and the valve body 21 facilitates the installation, replacement and separate operation of the culture bottle 3; the stable gas environment and precise flow control ensure the consistency of CO2 production and exchange during the soil organic carbon mineralization process, making the long-term culture data more repeatable and comparable, and providing a more reliable experimental basis for organic carbon mineralization analysis. The present invention has a simple structure, is easy to use, has low experimental cost, can accurately control the test data during the test process, improves the repeatability and reliability of the test, and improves the accuracy of soil organic carbon mineralization analysis. It is suitable for long-term or large-scale organic carbon mineralization experiments.

[0044] In one embodiment of the present invention, the inner cavity of the valve body 21 is connected to several branch air pipes 24, and the air supply valve 23 is set on the branch air pipe 24 to control the gas outflow speed of the branch air pipe 24; the air supply pipe 22 is connected to the outlet of the branch air pipe 24.

[0045] In one embodiment of the present invention, the number of the branch air pipes 24 is preferably 26, which are symmetrically distributed on both sides of the valve body 21.

[0046] In a further optimized solution, the culture bottle 3 includes a transparent bottle body 31 with a detachable bottle stopper 32, and an air inlet head 33 and an air outlet head 34 are respectively plugged into the bottle stopper 32. The transparent bottle body 31 facilitates observation of the growth of the soil sample. The bottle stopper 32 is made of rubber and is detachably mounted at the mouth of the bottle body 31, making it convenient for early soil filling, water adjustment, and later cleaning, simplifying operation. The air inlet head 33 and the air outlet head 34 are respectively plugged into the bottle stopper 32 and communicate with the inner cavity of the bottle body 31, ensuring a reliable seal in the gas channel, reducing the risk of gas leakage, and ensuring accurate CO2 collection. The bottle body 31 and bottle stopper 32 are detachable, making the culture bottle 3 easy to clean and reuse, and also convenient for replacing the air inlet head 33 and air outlet head 34 of different sizes to meet different experimental needs.

[0047] To further optimize the solution, the air inlet head 33 is inserted into the bottle stopper 32 at an angle, and the air outlet head 34 is inserted into the bottle stopper 32 vertically. The inner diameter of the air inlet head 33 is smaller than that of the air outlet head 34. The inclined air inlet head 33 design allows the incoming gas to flow along the inner wall of the bottle body 31, avoiding direct impact on the soil and causing dust, reducing interference with the soil microbial environment, and helping to more evenly distribute the air in the soil sample. The vertical outlet head 34 with a larger inner diameter facilitates the rapid discharge of gas in the bottle and improves gas exchange efficiency. The different designs of the air inlet head 33 with an inner diameter smaller than the air outlet head 34 can adjust the rate of air entry and exhaust, ensure stable pressure in the bottle body 31, and prevent gas stagnation from affecting CO2 measurement accuracy, thereby meeting the needs of different experimental conditions.

[0048] In one embodiment of the present invention, the air inlet and the air outlet are both three-way settings, an air inlet valve 35 is provided on the air inlet, and an air outlet valve 36 is provided on the air outlet, which can flexibly control the air intake and exhaust of the culture bottle 3 and accurately control the parameters of the test process.

[0049] To further optimize the solution, an air inlet pipe 11 is provided between the air outlet of the air compressor 1 and the valve body 21. A pressure gauge 12 is connected to the air inlet pipe 11, and the pressure gauge 12 is electrically connected to a control box 16 provided on the air compressor 1. The air compressor 1 is connected to the valve body 21 via the air inlet pipe 11, facilitating the transfer of compressed air. The design of the pressure gauge 12 allows the experimenter to monitor the output pressure of the air compressor 1 in real time, and the data is fed back to the control box 16 to achieve dynamic pressure regulation, avoiding unstable air supply caused by excessively high or low pressure, thereby ensuring the stability of the air flow. At the same time, the electrical connection between the pressure gauge 12 and the control box 16 enables automatic adjustment of the output pressure of the air compressor 1, reducing manual intervention, lowering operational errors, ensuring consistent gas pressures at different culture stages, further improving the accuracy and automation of the experiment, and enhancing experimental repeatability.

[0050] To further optimize the solution, the air compressor 1 includes a gas cylinder 14, an air inlet pipe 11 is arranged between the gas cylinder 14 and the valve body 21, the inlet of the gas cylinder 14 is connected to the compressor 15, and the compressor 15 is electrically connected to the control box 16. The compressor 15 can compress the air and store it in the gas cylinder 14, buffering the gas output pressure, further stabilizing the gas supply flow, and avoiding the pulse-like fluctuation of the direct gas supply from the compressor 15; it can also ensure that even if the air compressor 1 fails during the experiment, the air supply can be maintained for a period of time; at the same time, the electrical connection between the compressor 15 and the control box 16 realizes real-time monitoring and adjustment of the working status of the compressor 15, automatically starting and stopping according to the pressure of the gas cylinder 14, reducing energy consumption, while ensuring a continuous supply of gas to meet long-term cultivation needs, ensuring the stable operation of the air compressor 1 and the continuity of the air flow.

[0051] In one embodiment of the present invention, the air compressor 1 is further integrated with a heat dissipation module 17 for dissipating heat from the gas cylinder 14 to ensure stable operation of the equipment.

[0052] In one embodiment of the present invention, the integrated design of the air compressor 1 improves the degree of automation of the device, reduces the intensity of manual operation, and meets the needs of efficient experiments.

[0053] The present invention also discloses an organic mineralization analysis method based on the improved soil culture device, comprising the following steps:

[0054] Add fresh soil and sterile water to culture bottle 3 and adjust the humidity to the required experimental level; weigh 10 g of fresh soil in dry weight into culture bottle 3 and add sterile water to adjust the moisture content to 60% of the maximum field capacity;

[0055] The culture bottle 3 containing soil was sealed with a biofilm and pre-cultured at 25°C in the dark for 7 days to stabilize the activity of soil microorganisms;

[0056] After pre-incubation, culture flasks 3 continued to be incubated, and CO2 gas was collected regularly during the incubation process. The soil moisture was kept constant during the incubation process. All culture flasks 3 were incubated in the dark at 25°C for 40 days, and CO2 gas was collected on days 1, 2, 4, 7, 14, 21, 28, 35, and 40. During the incubation period, an appropriate amount of sterile ultrapure water was added to maintain a constant soil moisture.

[0057] The air inlet 33 and the air outlet 34 are connected to the outside world to maintain ventilation, so that the soil respiration is stable. The air outlet made of a thick needle and the air inlet made of a thin needle are inserted into the bottle stopper 32 at the same time, with the air inlet inserted at an angle and the air outlet inserted vertically. The air inlet valve 35 and the air outlet valve 36 are opened to maintain ventilation at the air inlet and outlet. Then, the soil is left to stand at the incubation temperature for about 1 hour to stabilize the soil respiration.

[0058] Ventilate the culture bottle 3 so that the air provided by the air compressor 1 replaces the gas in the culture bottle 3; connect the air supply pipe 22 to the air inlet of the culture bottle 3, open the air compressor pump control main valve 13 and the corresponding air supply valve 2; then adjust the air inlet flow rate to keep the flow rate of different air supply pipes 22 consistent; at the same time, make the displacement of the air outlet greater than the air supply of the air inlet, and ventilate for 15 minutes to discharge the original carbon dioxide in the culture bottle 3; when the ventilation time is reached, first close the air outlet valve 36 on the air outlet head 34 of the culture bottle 3, and then close the air outlet valve 35 on the air inlet head 33 to ensure that all culture bottles 3 are in a sealed state; finally, close the control main valve 13 and air supply valve 2 to end the ventilation;

[0059] Continue to cultivate the soil in the culture bottle 3, then mix the gas in the culture bottle 3 evenly and then extract the gas for measurement; place the culture bottle 3 in a 25°C incubator in the dark and cultivate for 2 hours; after the incubation time is up, remove the culture bottle 3, connect the gas outlet with a syringe, open the gas outlet valve 36, push the piston up and down 2-3 times to mix the gas in the culture bottle 3 evenly, then extract about 25 ml of gas from the culture bottle 3, close the gas outlet valve 36, pull out the syringe, and inject the gas in the syringe into a 25 ml air bag; use a gas chromatograph to measure the CO2 peak area, and calculate the sample carbon dioxide concentration based on the measured standard curve and background gas concentration.

[0060] In one embodiment of the present invention, the culture bottle 3 is sealed with a biofilm, which can reduce evaporation while ensuring normal respiration of soil microorganisms in the bottle.

[0061] To further optimize the plan, it is necessary to test the soil moisture content and determine the soil field water holding capacity before the experiment to obtain soil data to facilitate subsequent experiments.

[0062] The steps for determining soil water content (SWC) are as follows:

[0063] Use an electronic balance to weigh the aluminum box M1, weigh about 5g of fresh sample, place it in the aluminum box, and weigh the total weight of the aluminum box and sample M2. Place it in an oven at 65℃ and bake for more than 12 hours until the weight is constant. Weigh the total mass of the dry soil and the aluminum box M3.

[0064] Calculation formula: SWC = (M2-M3) / (M3-M1)

[0065] Where SWC is the soil water content; M1 is the weight of the aluminum box; M2 is the total weight of the aluminum box and fresh soil; and M3 is the total weight of the aluminum box and dry soil.

[0066] The steps for determining soil field water holding capacity (WHC) are as follows:

[0067] Fold the filter paper and put it into the funnel, seal the bottom of the funnel with a sealing film to ensure that it is leak-proof, and place the funnel on the conical flask.

[0068] Soak the filter paper with a small amount of distilled water so that it sticks tightly to the wall of the funnel. Use a medicine spoon to take 3-5g of fresh soil sample and put it into the funnel. Add distilled water to cover the soil sample by about 1cm. Seal the mouth of the funnel with plastic wrap to prevent water evaporation. Poke a few holes with a dissecting needle to prevent microbial metabolism in an anaerobic environment. Let it stand for 12 hours.

[0069] After 12 hours, open the sealing film at the bottom of the funnel and the plastic wrap on the surface to filter out all the excess water. At this time, the soil is saturated with water.

[0070] Use a medicine spoon to take 2-3g of water-saturated soil from the funnel and weigh it. Place it in the weighed aluminum box M1 to obtain the total weight of the aluminum box and fresh soil M2. Place it in a 65℃ oven for more than 12 hours to a constant weight. Take out and weigh the total weight of the aluminum box and dry soil M3.

[0071] Calculation formula: WHC = (M2-M3) / (M3-M1)

[0072] Where WHC is the maximum field water holding capacity of the soil; M1 is the weight of the aluminum box; M2 is the total weight of the aluminum box and fresh soil; M3 is the total weight of the aluminum box and dry soil.

[0073] A further optimization scheme involves closing the gas inlet 33 of each culture bottle 3 and then sampling through the gas outlet 34 to measure the CO2 content of the gas. One minute before the end of ventilation of the culture bottles 3, 5-6 culture bottles 3 are randomly selected. A syringe is inserted through the gas outlet, and the gas outlet valve 36 is opened. The syringe piston is gently pulled out. Once the syringe is filled with gas, the gas outlet valve 36 is closed and the syringe is removed.

[0074] In one embodiment of the present invention, when ventilating the culture bottle 3, it is necessary to check whether it is fully ventilated. When checking, the inlet is blocked with a finger and suddenly released to hear the sound of airflow.

[0075] In the description of the present invention, it should be understood that the terms "longitudinal", "transverse", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0076] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. An improved soil cultivation device, characterized in that: include: An air compressor (1), wherein the air compressor (1) is used to provide a stable air source during the experiment; An air supply valve (2), the air supply valve (2) comprising a valve body (21), an inlet of the valve body (21) being in communication with an outlet of the air compressor (1), and a plurality of independently arranged air supply pipes (22) being provided on the valve body (21); A culture bottle (3) is provided with an independently arranged air inlet head (33) and an air outlet head (34), the air inlet head (33) and the air outlet head (34) being respectively communicated with the inner cavity of the culture bottle (3); the air inlet head (33) is detachably connected to the air supply pipe (22).

2. The improved soil cultivation device according to claim 1, characterized in that: The culture bottle (3) comprises a transparent bottle body (31), a detachable bottle stopper (32) is provided on the bottle body (31), and the air inlet head (33) and the air outlet head (34) are respectively plugged into the bottle stopper (32).

3. The improved soil cultivation device according to claim 2, characterized in that: The air inlet head (33) is plugged into the bottle plug (32) at an angle, and the air outlet head (34) is plugged into the bottle plug (32) vertically. The inner diameter of the air inlet head (33) is smaller than the inner diameter of the air outlet head (34).

4. The improved soil cultivation device according to claim 1, characterized in that: An air inlet pipe (11) is provided between the air outlet of the air compressor (1) and the valve body (21), and a pressure gauge (12) is connected to the air inlet pipe (11). The pressure gauge (12) is electrically connected to a control box (16) provided on the air compressor (1).

5. The improved soil cultivation device according to claim 4, characterized in that: The air compressor (1) includes a gas cylinder (14), the air inlet pipe (11) is arranged between the gas cylinder (14) and the valve body (21), the inlet of the gas cylinder (14) is connected to the compressor (15), and the compressor (15) is electrically connected to the control box (16).

6. An organic mineralization analysis method, according to the improved soil cultivation device according to any one of claims 1 to 5, characterized in that The following steps are involved: Add fresh soil and sterile water to the culture bottle (3) and adjust the humidity to the required experimental level; The culture bottle (3) filled with soil is sealed with a biofilm and then cultured under a set environment to stabilize the activity of soil microorganisms; The culture bottle (3) after pre-culture continues to be cultured, and CO2 gas is collected regularly during the culture process, and the moisture content of the culture soil is kept constant during the culture process; The air inlet (33) and the air outlet (34) are respectively connected to the outside world to maintain a ventilation state, so that the soil respiration is stable; Ventilating the culture bottle (3) so that the air provided by the air compressor (1) replaces the gas in the culture bottle (3); The air inlet (33) and the air outlet (34) are respectively kept in a closed state with the outside world, and the soil in the culture bottle (3) is continuously cultured. Then, the gas in the culture bottle (3) is mixed evenly and the gas is extracted for measurement.

7. The organic mineralization analysis method according to claim 6, characterized in that: Before the test, it is necessary to test the soil moisture content and determine the soil field water holding capacity to obtain soil data to facilitate subsequent tests.

8. The organic mineralization analysis method according to claim 6, characterized in that The ventilation of the culture bottle (3) comprises the following steps: Start the air compressor (1) and introduce air into the culture bottle (3) through the air inlet head (33); Open the gas outlet (34) and adjust the gas outlet speed so that the gas inlet and outlet speeds of the culture bottle (3) are the same; The culture bottle (3) is continuously ventilated to completely expel the air in the culture bottle (3); First, the air outlet (34) of the culture bottle (3) is closed, and then the air inlet (33) of the culture bottle (3) is closed, so that the culture bottle (3) is in a closed state.

9. The organic mineralization analysis method according to claim 8, characterized in that: After closing the gas inlet (33) of the culture bottle (3), a sample is taken through the gas outlet (34) and retained to measure the CO2 content of the gas filled in.

10. The organic mineralization analysis method according to claim 6, characterized in that: The step of uniformly mixing the gas in the culture bottle (3) comprises: Connect the syringe to the gas outlet head (34); Open the gas outlet valve (36) on the gas outlet head (34), and draw in and out the syringe several times to mix the gas in the culture bottle (3) evenly; close the gas outlet valve (36), and then remove the syringe.

Images