Mycobacterium madri and its application in treating developing sewage

Abstract

The present application relates to the technical field of microbial treatment of sewage, and particularly relates to a strain of Mycobacterium madri and application thereof in treatment of developing sewage, which is classified and named as Mycolicibacterium mageritense ​ WH05, and the preservation number is CCTCC NO: M2025751. The Mycobacterium madri can utilize tetramethylammonium hydroxide as a carbon source and a nitrogen source, can grow at a concentration of 3000 mg / L TMAH, can effectively degrade tetramethylammonium hydroxide in electronic factory sewage, has a highest tolerance salinity of 1.5 wt%, and can realize complete biodegradation of tetramethylammonium hydroxide in sewage in combination with Nitrosomonas CZ-4.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial treatment of sewage, and particularly relates to a Mycobacterium madrii and application thereof in treatment of developing sewage. BACKGROUND

[0002] Developing wastewater is one of the main wastewaters generated in the process of microelectronic manufacturing, and contains a large amount of tetramethylammonium hydroxide (TMAH, 1000-9000 mg / L). The TMAH has strong toxicity and potential harm to the environment and organisms. Although there is no restriction provision in the current domestic sewage discharge standard, high TMAH concentration (> 150 mg / L) will directly lead to total nitrogen and total carbon exceeding the standard and affect the activity of other microorganisms in the sewage biochemical treatment process, thereby indirectly causing abnormality of various indexes of effluent. The existing TMAH treatment methods include physical, chemical and biological methods, but the chemical and physical treatment has high cost, low efficiency and may cause secondary pollution. Therefore, it is of great significance to find microbial resources capable of efficiently degrading TMAH and apply them to sewage treatment.

[0003] In the current research on biological degradation of TMAH, the anaerobic microbial degradation rate is much higher than that of aerobic microorganisms, but the anaerobic degradation process is limited in practical application due to the production of methane. Most of the domestic and foreign researches on aerobic TMAH-degrading microorganisms are focused on the analysis and application of the composition of activated sludge, and there are few researches on the separation and application of pure strains for TMAH degradation. There is no related research or patent report on the application of Mycobacterium madrii to the degradation of TMAH in developing wastewater of chip manufacturing. SUMMARY

[0004] Therefore, the present application screens and purifies a Mycobacterium madrii WH05 which exhibits significant TMAH degradation capacity under aerobic conditions, can be directly applied to the treatment of TMAH wastewater in chip manufacturing, degrades TMAH to produce ammonia nitrogen, improves the biodegradability of sewage, and can combine with Nitrosomonas sp. CZ-4 to treat the ammonia produced by TMAH degradation, so as to realize complete biological degradation of TMAH and avoid the influence of degradation byproducts on normal sewage treatment. Nitrosomonas

[0005] The technical scheme of the present application is as follows:

[0006] On one hand, the present application provides a Mycobacterium madrii WH05. The strain is preserved in China Center for Type Culture Collection (address: Wuhan University, Wuhan, China) on April 11, 2025, and the classification name is Mycobacterium madrii WH05, the preservation number is CCTCC NO: M 2025751. Mycolicibacterium mageritense WH05, the preservation number is CCTCC NO: M 2025751. ​

[0007] The above-mentioned Mycobacterium madri WH05 is an environmental adaptable facultative anaerobic microorganism, which can utilize methanol, glucose, fructose, tetramethylammonium hydroxide as carbon source, and sodium nitrite, potassium nitrate, urea, tetramethylammonium hydroxide as nitrogen source for rapid growth.

[0008] In a second aspect, the application provides the use of the Mycobacterium madri WH05 as described above in treating developing wastewater.

[0009] On the basis of the above technical solution, preferably, the strain Mycobacterium madri WH05 is inoculated into developing wastewater containing tetramethylammonium hydroxide to degrade tetramethylammonium hydroxide.

[0010] On the basis of the above technical solution, further preferably, the Mycobacterium madri WH05 produces ammonia in the process of utilizing urea and tetramethylammonium hydroxide as nitrogen source, and can grow in inorganic salt culture medium with 100-3000 mg / L TMAH as the only nitrogen source, with different degrees of TMAH degradation effect, and the degradation rate of 500 mg / L TMAH reaches 91.51%.

[0011] On the basis of the above technical solution, preferably, the strain further contains Nitrosomonas CZ-4, which has been reported to remove ammonia nitrogen in wastewater.

[0012] On the basis of the above technical solution, preferably, the concentration of tetramethylammonium hydroxide in the wastewater is ≤3000 mg / L.

[0013] On the basis of the above technical solution, preferably, the salinity of the wastewater is 0.3-1.5 wt%.

[0014] On the basis of the above technical solution, preferably, the Mycobacterium madri WH05 degrades tetramethylammonium hydroxide in wastewater under aerobic conditions.

[0015] On the basis of the above technical solution, further preferably, the Mycobacterium madri WH05 is inoculated in electronic plant wastewater (TMAH concentration 613.05 mg / L) at 10 vol%, and 90.44% of TMAH can be removed in 24 h, and after TMAH is degraded to 150 mg / L, Nitrosomonas CZ-4 fermentation broth is added at 1 vol‰, which can remove all ammonia nitrogen produced by TMAH degradation.

[0016] In a third aspect, the application provides a microbial agent for degrading tetramethylammonium hydroxide, preferably comprising the above-mentioned Mycobacterium madri WH05.

[0017] The screened and purified Mycobacterium madri WH05 of the application has the following beneficial effects relative to the prior art:

[0018] The aerobic degradation microorganism of TMAH, Mycobacterium madri WH05, is screened and purified from activated sludge in sewage, Mycolicibacterium mageritense which can utilize tetramethylammonium hydroxide as the sole carbon source and nitrogen source to achieve degradation, thereby improving the biodegradability of sewage, and can reduce 613.05 mg / L of TMAH to 58.59 mg / L in 24 hours in electronic plant sewage containing TMAH, and the ammonia nitrogen produced by degradation can be combined with Nitrosomonas CZ-4 to be removed efficiently; the microorganism can also effectively reduce the concentration of TMAH in electronic plant sewage with a salinity of 1.3%, thereby providing a new solution for TMAH degradation in high-salinity sewage in practical applications. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0020] Figure 1 is a colony morphology diagram of Mycobacterium madri WH05 of the application on Middle Brook 7H10 medium;

[0021] Figure 2 is a microscope photo of Mycobacterium madri after acid-fast staining of the application;

[0022] Figure 3 is a growth curve of Mycobacterium madri WH05 of the application under different carbon sources;

[0023] Figure 4 is a growth curve of Mycobacterium madri WH05 of the application under different nitrogen sources;

[0024] Figure 5 is a change diagram of ammonia nitrogen of Mycobacterium madri WH05 of the application grown in urea and tetramethylammonium hydroxide;

[0025] Figure 6 is a growth curve diagram of Mycobacterium madri WH05 of the application in different concentrations of TMAH;

[0026] Figure 7 is a degradation rate diagram of Mycobacterium madri WH05 of the application on different concentrations of TMAH;

[0027] Figure 8 is the growth chart of Mycobacterium madri WH05 in different salinity medium of the application;

[0028] Figure 9 is the degradation chart of TMAH by Mycobacterium madri WH05 in different salinity medium;

[0029] Figure 10 is the chart of the change of electronic sewage water quality index and TMAH concentration without adding Mycobacterium madri WH05 and Nitrosomonas CZ-4;

[0030] Figure 11 is the chart of the change of electronic sewage water quality index and TMAH concentration with adding Mycobacterium madri WH05 first and then Nitrosomonas CZ-4;

[0031] Figure 12 is the chart of the comparison of biological oxygen consumption of sewage before and after the treatment by Mycobacterium madri WH05 of the application;

[0032] Figure 13 is the chart of the TMAH degradation effect and the change of ammonia nitrogen of 1.3% salinity electronic sewage by Mycobacterium madri WH05 of the application. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the application will be clearly and completely described in combination with the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments of the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application.

[0034] The preparation of the medium used in the following examples is as follows:

[0035] Inorganic salt basic medium: add the following ingredients to each 1 L of pure water: CaCl2·2H2O 0.5 g, FeSO4·7H2O 0.4 g, K2HPO4 1 g, MgSO4·7H2O 0.05 g, NaCl 2 g, NaHCO3 1.5 g, KNO3 0.8 g, trace element solution 1 mL; wherein the trace element solution: EDTA 0.5 g / L, CuSO4·5H2O 0.075 g / L, ZnSO4·7H2O 0.3 g / L, CoCl2·6H2O 0.375 g / L, MnCl2·2H2O 0.3 g / L, H3BO4 0.014 g / L, NaMoO4·2H2O 0.22 g / L. The solid medium is to add 20 g / L agarose in the basic medium. After preparation, sterilize at 121℃ for 20 min.

[0036] Middle Brook 7H10 Broth: To 900 mL of purified water, add the following ingredients: glycerol 5 mL, ammonium sulfate 0.5 g, potassium dihydrogen phosphate 1.5 g, sodium phosphate dibasic 1.5 g, sodium citrate 0.4 g, magnesium sulfate 0.025 g, calcium chloride 0.0005 g, zinc sulfate 0.001 g, copper sulfate 0.001 g, sodium L-glutamate 0.5 g, ferric ammonium citrate 0.04 g, pyridoxal hydrochloride 0.001 g, biotin 0.0005 g, malachite green 0.00025 g, sterilize at 121 °C for 10 min, cool to about 60 °C, and add 100 mL of sterilized filtered OADC supplement (bovine albumin (fraction V) 5 g, oleic acid 0.06 mL, glucose 2 g, catalase 0.003 g, sodium chloride 0.85 g).

[0037] The water quality indicators involved were detected by national standard methods, among which the ammonia nitrogen detection method adopted "Determination of Ammonia Nitrogen in Water - Nessler's Reagent Spectrophotometric Method" (HJ 535-2009), "Determination of Ammonia Nitrogen in Water - Flow Injection-Salicylic Acid Spectrophotometric Method" (HJ 666-2013); the determination of nitrite nitrogen adopted "Determination of Nitrite Nitrogen in Water - Spectrophotometric Method" (GB / T 7493-1987); the detection of nitrate nitrogen content adopted "Determination of Nitrate Nitrogen in Water - Ultraviolet Spectrophotometric Method" (HZ / T 346-2007); and the total nitrogen detection method adopted "Determination of Total Nitrogen in Water - Alkaline Persulfate Digestion Ultraviolet Spectrophotometric Method" (HJ 636-2012).

[0038] The TMAH concentration used in data analysis was calculated based on the organic nitrogen concentration, and the specific calculation method was as follows:

[0039]

[0040] Example 1: Isolation and purification of Mycobacterium madri WH05

[0041] The inorganic salt basic medium was added with methanol (COD 1500 mg / L) and tetramethylammonium hydroxide (1000 mg / L) as carbon source and nitrogen source, and the pH was adjusted to about 7.5. 5 mL of activated sludge taken from a biochemical treatment system of a wastewater treatment plant of a chip manufacturing enterprise in Wuhan, Hubei Province was placed in 100 mL of the inorganic salt medium, and was cultured at 30°C and 140 r / min on a shaker. The change of ammonia nitrogen concentration in the culture was continuously monitored. When the ammonia nitrogen content reached 100 mg / L, the enrichment culture was performed for 5 rounds. The inorganic salt basic medium was added with methanol (COD 1500 mg / L), tetramethylammonium hydroxide (1000 mg / L) and agarose 20 g / L, and the pH was adjusted to about 7.5. The solid separation plate was prepared. The enrichment culture was streaked on the solid separation plate, and was incubated at 30°C for 7 days. The single colony was picked to the new inorganic salt medium (methanol and tetramethylammonium hydroxide were added at the above-mentioned concentrations) for culture verification, and the target strain WH05 was obtained.

[0042] Example 2: Identification of Mycobacterium madri WH05

[0043] The purified culture was streaked on a Middle Brook 7H10 solid medium, and was incubated at 30°C for 5 days. The colony morphology photograph is shown in Figure 1 . It was observed that the colony was white, had no luster on the surface, and was easy to pick up. After the bacterial body was dyed by using Ziehl-Neelsen acid-fast staining method, it was observed under a microscope, and was detected as acid-fast positive. Figure 2 The single colony was inoculated in a Middle Brook 7H10 broth, and was incubated at 30°C for 3 days. The DNA was extracted, and the 16s rDNA and rpoB gene fragments were amplified. After sequencing, the species was identified by comparison. The primer sequences used for amplification are as follows:

[0044] 16s rDNA upstream primer 27F: 5'-AGAGTTTGATCMTGGCTCAG-3'.

[0045] 16s rDNA downstream primer 1492R: 5'-TACGGCTACCTTGTTACGACTT-3'.

[0046] rpoB upstream primer F: 5'-TCAAGGAGAAGCGCTACGA-3'.

[0047] rpoB downstream primer R: 5'-GGATGTTGATCAGGGTCTGC-3'.

[0048] The sequencing of the PCR product, and the 16s rDNA and rpoB gene sequencing results are shown in SEQ ID NO. 1 and SEQ ID NO. 2, respectively.

[0049]

[0050] 16s rDNA sequence after comparison in NCBI database shows that the isolated strain is Mycobacterium madriense Mycolicibacterium , and rpoB sequence comparison result shows that it is Mycobacterium madriense Mycolicibacterium mageritense (95.5%).

[0051] Example 3: Utilization of different carbon sources by Mycobacterium madriense WH05

[0052] Single colonies were picked on the plate and inoculated into inorganic salt medium, and the blank group was only added with TMAH 1000 mg / L as a nitrogen source, and other groups were added with TMAH 1000 mg / L and then added with sodium acetate, methanol, glucose and fructose (COD was 1500 mg / L) respectively, pH was adjusted to about 7.5, and the culture was carried out at 30°C and 140 r / min, and the ammonia nitrogen concentration was determined every 24 h. Because TMAH is converted into ammonia during its growth, the ammonia nitrogen concentration was used to observe its growth under different carbon sources, and its growth curve graph is shown in Figure 3 .

[0053] According to Figure 3 : Mycobacterium madriense WH05 began to grow obviously after 1 d in the medium with glucose + TMAH and fructose + TMAH as carbon sources, and the fructose + TMAH group entered the logarithmic growth phase faster; the TMAH and methanol + TMAH groups began to grow obviously after 2 d, and the methanol + TMAH group entered the logarithmic growth phase faster; the sodium acetate + TMAH group had no change in ammonia nitrogen, and the culture solution was clear and transparent according to observation. The above results show that Mycobacterium madriense WH05 can use methanol, glucose, fructose and TMAH as carbon sources for growth, and grows best with fructose, and is inhibited in the presence of sodium acetate.

[0054] Example 4: Utilization of different nitrogen sources by Mycobacterium madriense WH05

[0055] The culture was inoculated into inorganic salt medium at 1% v / v, fructose (COD 1500 mg / L) was added as a carbon source, and ammonium chloride, sodium nitrite, potassium nitrate, urea and TMAH were added to make the nitrogen source concentration be 160 mg / L, and pH was adjusted to about 7.5, and the culture was carried out at 30°C and 140 r / min, and the OD 600 and ammonia nitrogen concentration were determined every 24 h, and its growth curve graph is shown in Figure 4 and 5 .

[0056] According to Figure 4 , Figure 5: Mycobacterium madriense WH05 started to grow obviously after 1 d in inorganic salt medium with sodium nitrite, potassium nitrate, urea, TMAH as nitrogen source, the initial growth rate was optimal with potassium nitrate, and the ammonia nitrogen increased obviously in the growth process with urea and TMAH; it started to grow obviously after 3 d in inorganic salt medium with ammonium chloride as nitrogen source. The above results show that Mycobacterium madriense WH05 can grow with ammonium chloride, sodium nitrite, potassium nitrate, urea, TMAH as nitrogen source, and grow slowly with ammonium chloride as nitrogen source, and convert urea and TMAH into ammonia when using these organic nitrogen sources.

[0057] Example 5: Tolerance and degradation efficiency of Mycobacterium madriense WH05 to TMAH

[0058] Single colony was picked and inoculated in Middle Brook 7H10 broth at 30℃ for 2 d, the bacterial liquid was centrifuged at 8000 r / min for 3 min, the supernatant was discarded, and the bacterial body was resuspended in equal amount of inorganic salt medium. Different concentrations of TMAH (100, 500, 1000, 2000, 3000 mg / L) were configured as nitrogen source, and fructose was configured as carbon source in inorganic salt medium, and the carbon-nitrogen ratio was fixed at 10, the bacterial liquid was inoculated at 1% v / v, the blank group was not inoculated with bacterial liquid, and the culture was carried out at 30℃, 140 r / min on a shaking table, and the sample was taken every 24 h to detect OD 600 , ammonia nitrogen, and soluble total nitrogen, and the TMAH concentration was calculated according to the ammonia nitrogen and soluble total nitrogen, and the results are shown in Figure 6 and Figure 7 .

[0059] According to Figure 6 , Figure 7 : the OD 600 of Mycobacterium madriense WH05 in inorganic salt medium with 500, 1000, 2000 mg / L TMAH was all >0.1 after 1 d of culture, the OD 600 of the group with 3000 mg / L was consistent with the initial value, only 0.027; all had obvious growth after 2 d of culture, and then entered the stable period, and the higher the final TMAH concentration, the more biomass accumulated; for 100 mg / L TMAH, it could be degraded to below 50 mg / L after 3 d; for 500 mg / L TMAH, it could be degraded to below 150 mg / L after 3 d, and the degradation rate reached 91.51% after 7 d. The above results show that Mycobacterium madriense WH05 will have growth delay at high concentration of TMAH (3000 mg / L), but it can grow and remove TMAH in the concentration range of 100-3000 mg / L.

[0060] Example 6: Growth and TMAH degradation of Mycobacterium madriense WH05 under different salinity

[0061] The Madurella mycetomi WH05 slope culture was inoculated in Middle Brook 7H10 broth at 30°C for 2d to obtain inoculum, and then the inorganic salt medium with TMAH as nitrogen source and fructose as carbon source was prepared with different salinity (0.3%, 1.0%, 1.5%, 2.0% NaCl), and the inoculum was added at 1% v / v, and the culture was incubated at 30°C and 140r / min for 3d, and then the sample was taken for determination of OD 600 , ammonia nitrogen, and soluble total nitrogen, and the TMAH concentration was calculated according to ammonia nitrogen and soluble total nitrogen, and the results are shown in Figure 8 and Figure 9 .

[0062] According to Figure 8 , Figure 9 : the OD 600 of Madurella mycetomi WH05 cultured at 0.3%, 1.0%, and 1.5% salinity for about 3.5d (87h) was 1.035, 0.913, and 0.828, respectively, and the OD 600 of the 1.0% salinity group was 11.19% lower than that of the 0.3% group, and the OD 600 of the 1.5% salinity group was 20% lower than that of the 0.3% group; the OD 600 of WH05 cultured at 2.0% salinity had no significant difference with the initial value, indicating that the growth of Madurella mycetomi WH05 was completely inhibited at 2.0% salinity; the degradation rate of TMAH by WH05 cultured at 0.3%, 1.0%, 1.5% salinity for 87h was 91.16%, 90.41%, 83.23%, and 8.41%, respectively, indicating that the normal growth of Madurella mycetomi WH05 at 0.3%-1.5% salinity could degrade TMAH, and the normal growth and degradation of TMAH were not possible at 2.0% salinity.

[0063] Example 7: TMAH degradation and biodegradability improvement effect of Madurella mycetomi WH05 on electronic plant wastewater

[0064] The experimental group added Madurella mycetomi WH05 (OD 600The TMAH degradation experiment was carried out as follows: 1000 mg / L of fructose was added as a carbon source, and 20% of activated sludge from an electronic factory was inoculated; the blank control group (CK group) was not inoculated with bacteria, and 20% of activated sludge from the electronic factory was inoculated, and the culture was incubated at 30°C and 140 r / min on a shaking table. Ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, and soluble total nitrogen were detected every day, and the TMAH concentration was calculated. When the TMAH concentration decreased to below 150 mg / L, the water was allowed to stand overnight, and all the supernatant was removed. New electronic factory wastewater was added, and the culture was incubated at 30°C and 140 r / min on a shaking table. Ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, and soluble total nitrogen were detected every day, and the TMAH concentration was calculated. When the TMAH concentration decreased again to below 150 mg / L, half of the volume of the water after the experiment was added with a nitrosomonas CZ-4 fermentation concentrate (nitrification rate of about 400 mg / L / h) at a ratio of 1 ‰ v / v, and a pH automatic control device was added to maintain the pH at 7-8. Ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen were measured every day. The other half of the water after the above TMAH degradation experiment was allowed to stand for 1 h, and the supernatant was inoculated with 20% of municipal wastewater activated sludge. Inorganic salts, ammonia nitrogen, and sodium acetate (the specific concentrations refer to the dilution and inoculation method for the determination of BOD5 in HJ505-2009) were added. Aeration was carried out for 1 h to make the initial dissolved oxygen about 6.0. The change in dissolved oxygen within 1 h was recorded. The oxygen consumption of the microorganisms in the wastewater reflected the biodegradability of the wastewater. The experimental results are shown in Figs. 1-12. Figure 10 11

[0065] According to the results shown in Figs. 1-12, Figure 10 Figure 11 Figure 12 Madrid Mycobacterium WH05 can reduce the TMAH in electronic factory wastewater, and can degrade it to below 150 mg / L within 24 h. During the degradation process, the ammonia nitrogen concentration in the wastewater increases, and the soluble total nitrogen decreases. After the TMAH is reduced, the ammonia nitrogen increases to 75.31 mg / L, and after the addition of nitrosomonas CZ-4, the ammonia nitrogen decreases to undetectable within 31 h. The oxygen consumption of the water treated in the experimental group is higher than that of the blank control group within the same time, indicating that the biological activity is higher, and the biodegradability of the water in the experimental group is better. The above results show that Madrid Mycobacterium WH05 can grow normally in electronic factory wastewater containing 700 mg / L of TMAH, can play a role in degrading TMAH, can improve the biodegradability of electronic factory wastewater, and can remove the ammonia nitrogen produced after degradation by nitrosomonas CZ-4.

[0066] Example 8: TMAH degradation of Madrid Mycobacterium WH05 in 1.3% salinity electronic factory wastewater

[0067] Figure 13 ​​​​The implementation method is: adding sodium chloride to the electronic factory wastewater to adjust the salinity to 1.3%, the experimental group (TEST) adds Mycobacterium madri WH05 bacterial liquid (OD 600 1.0) to the electronic factory wastewater according to 1% v / v, and adds fructose 1000 mg / L; the blank control group (CON) only adds 1000 mg / L fructose, and is cultured at 30 DEG C and 140 r / min of a shaking table, and the ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and soluble total nitrogen are detected by sampling at intervals, and the TMAH concentration is calculated.

[0068] The experimental results are as shown in Figure 13 After Mycobacterium madri WH05 is added to the electronic factory wastewater with 1.3% salinity and cultured for 6 days, the TMAH concentration is decreased by 53.03%, and the ammonia nitrogen is increased, which indicates that Mycobacterium madri WH05 can still grow in the electronic factory wastewater with 1.3% salinity, and the TMAH concentration is reduced through ammonification.

[0069] The above only describes the preferred embodiments of the present application and is not used to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A Mycobacterium madrii strain, characterized in that: The strain is Mycolicibacterium mageritense WH05, with the preservation number of CCTCC NO: M 2025751.

2. Use of Mycolicibacterium mageritense according to claim 1 for treating developing sewage.

3. Use according to claim 2, wherein: Strains Mycolicibacterium mageritense WH05 was inoculated into the developing sewage containing tetramethylammonium hydroxide, and degraded the tetramethylammonium hydroxide.

4. Use according to claim 3, wherein: Strains Mycolicibacterium mageritense WH05 and strain Nitrosomonas CZ-4 were inoculated into the developing sewage containing tetramethylammonium hydroxide, and tetramethylammonium hydroxide was degraded and ammonia nitrogen was removed by adding Mycolicibacterium mageritense WH05, then adding Nitrosomonas CZ-4.

5. The use according to claim 2, characterized in that: The concentration of tetramethylammonium hydroxide in the sewage is ≤ 3000 mg / L.

6. The use according to claim 2, characterized in that: The salinity of the sewage is 0.3-1.5 wt%.

7. A microbial agent that degrades tetramethylammonium hydroxide, characterized by: The sewage is treated with Mycolicibacterium mageritense according to claim 1.

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