Skin-tendering and brightening composition containing camellia nitidissima leaf essential oil and preparation method and application of skin-tendering and brightening composition

Abstract

The invention relates to a skin tendering and brightening composition containing camellia chrysantha leaf essential oil and a preparation method and application of the skin tendering and brightening composition. The composition comprises the following components: camellia chrysantha leaf essential oil, ascorbic acid polypeptide, oat beta-glucan, camellia seed oil and tiger nut oil, the weight ratio of the camellia chrysantha leaf essential oil to the ascorbic acid polypeptide to the oat beta-glucan to the camellia seed oil to the tiger nut oil is (0.1 to 10) to (0.01 to 1) to (0.01 to 5) to (0.01 to 5) to (0.01 to 5); wherein the camellia chrysantha leaf essential oil is obtained by performing eurotium cristatum fermentation on camellia chrysantha leaves and then performing supercritical CO2 extraction; according to the composition disclosed by the invention, the components have a synergistic effect, inflammatory roughness and darkness are synchronously blocked, the limitation that a traditional product only aims at anhydrotic roughness is broken through, and a comprehensive solution without hormone stimulation is provided for sensitive skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin-rejuvenating and brightening composition containing golden camellia tea essential oil, and a preparation method and application thereof. Background Art

[0002] The current skincare products market focuses on roughness caused by dryness to address the problem of skin roughness and dullness, but this is not enough. Roughness caused by inflammation is more serious and more difficult to recover, and it can also lead to dullness. On the one hand, inflammation causes the skin to become dark and yellow, and on the other hand, rough skin and an uneven stratum corneum affect the reflection of light on the skin, causing the skin to become dull. Most skincare products focus on roughness caused by dehydration (such as adding hyaluronic acid and ceramides to replenish moisture), but they cannot block the inflammatory cascade. Although synthetic anti-inflammatory ingredients (such as hormone ointments) can suppress erythema in the short term, they increase the risk of barrier damage and pigmentation; and traditional plant oils (such as ordinary camellia oil) have a large molecular weight and poor permeability, making it difficult to inhibit deep inflammatory signaling, resulting in repeated attacks of inflammatory roughness and continued worsening of dullness. In addition, conventional extraction processes (such as hot pressing) easily destroy active ingredients, leading to oxidative rancidity of the oil, requiring the addition of chemical preservatives (such as phenoxyethanol) to further induce irritation. Summary of the Invention

[0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a skin-rejuvenating and brightening composition containing golden camellia tea essential oil, as well as a preparation method and application thereof.

[0004] To achieve the above object, the technical solution adopted by the present invention is:

[0005] In a first aspect, the present invention provides a skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising the following components: golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil, wherein the weight ratio of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is (0.1-10): (0.01-1): (0.01-5): (0.01-5): (0.01-5);

[0006] The method for preparing the golden camellia tea essential oil comprises the following steps:

[0007] S1, solid-state fermentation of Camellia chrysantha and Eurotium cristatum;

[0008] S2. Extracting the fermented golden camellia tea leaves with supercritical CO2 to obtain the golden camellia tea essential oil.

[0009] The functions of the raw materials of the components of the skin-rejuvenating and brightening composition of the present invention are as follows:

[0010] Golden Flower Tea essential oil is extracted from the tea leaves of the tea family through solid-state fermentation of Eurotium cristatum. Through microbial metabolic transformation, the large-molecule polyphenols in the tea are degraded into small-molecule theabrownins and flavonoid glycosides (such as fuzhuanin A), significantly enhancing antioxidant and anti-inflammatory activities. The supercritical CO2 extraction process retains volatile terpenes (such as linalool), imparting a natural floral fragrance and preventing oil rancidity. Golden Flower Tea essential oil inhibits the TNF-α inflammatory pathway, reduces erythema and abnormal keratin differentiation, scavenges free radicals, blocks pigmentation, and simultaneously improves inflammatory roughness and dullness, making it suitable for sensitive skin repair.

[0011] As a stable derivative of vitamin C, ascorbic acid peptides integrate L-ascorbic acid with a polypeptide chain, overcoming the oxidative inactivation of traditional vitamin C. They inhibit tyrosinase activity, blocking the melanin synthesis pathway and brightening the skin tone. Furthermore, they activate collagen synthesis in fibroblasts, improving skin texture and elasticity. Their small molecule properties support transdermal absorption, making them gentle and non-irritating, making them suitable for repairing dullness and acne scars caused by photoaging without the risk of photosensitivity.

[0012] Oat β-glucan, a natural polysaccharide extracted from oat bran, has a unique linear structure of β-(1→3,1→4) glycosidic bonds, forming a highly viscous, hydrated film. This creates a breathable, moisture-retaining layer on the skin's surface, reducing transepidermal water loss (TEWL). It also activates macrophages to release epidermal growth factor, promoting keratinocyte migration and barrier repair. Its anti-inflammatory properties can alleviate neurogenic pruritus, making it suitable for deep moisturizing and barrier repair in dry and sensitive skin, particularly for dehydration-related roughness caused by over-cleansing.

[0013] Camellia seed oil, derived from the cold-pressed oil of Camellia oleifera seeds, is rich in oleic acid (78-86%) and squalene, with a fatty acid ratio highly similar to human sebum. It rapidly penetrates the skin to replenish intercellular lipids and repair the lipid bilayer structure. Oleic acid softens hardened keratin and improves uneven texture. Furthermore, tea polyphenols provide synergistic antioxidant properties, reducing UV-induced lipid peroxidation. It is suitable for dry, chapped skin and aging-related roughness, and can be used as a massage oil to enhance skin's suppleness.

[0014] Tiger nut oil, a plant oil extracted from tiger nuts (Cyperus esculentus), boasts a core active ingredient: tocopherol (vitamin E) and a complex of oleic and linoleic acids, boasting significant antioxidant properties. Its small-molecule lipids penetrate the stratum corneum, activating peroxisome proliferator-activated receptors (PPARγ), promoting the expression of barrier proteins (such as filaggrin), and enhancing moisture retention. It also inhibits matrix metalloproteinase (MMP-1) activity and slows collagen degradation. It is suitable for repairing dullness and photodamage caused by oxidative stress, and can synergize with sunscreen products to enhance photoprotection.

[0015] Preferably, the weight ratio of the golden camellia tea essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil is (0.5-5): (0.05-0.5): (0.1-3): (0.1-1): (0.05-0.5).

[0016] More preferably, the weight ratio of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil is (1-2): (0.08-0.15): (0.5-1): (0.2-0.5): (0.1-0.2).

[0017] Preferably, in the preparation step S1 of the golden camellia tea essential oil, the steps include:

[0018] S11, inoculate the cristatum onto PDA solid medium for cultivation, add sterile water to wash the culture, and then evenly disperse it by vortexing and shaking to obtain spore liquid, and adjust the spore liquid concentration to 10 with sterile water. 6 -10 8 / mL;

[0019] S12: Wash the fresh leaves of Camellia chrysantha and dry them until the moisture content is 10-20%;

[0020] S13: Evenly mixing the spore liquid and the dried golden camellia tea leaves, adding 10-30 mL of the spore liquid to every 100 g of tea leaves, and performing solid fermentation under closed conditions to obtain the fermented golden camellia tea leaves; wherein the solid fermentation temperature is 26-32° C., the humidity is 80-85%, and the fermentation time is 8-20 days.

[0021] Preferably, the spore liquid content in every 100 g of tea leaves is 20-30 mL, the solid fermentation temperature is 28-30° C., and the fermentation time is 10-12 days.

[0022] Preferably, in the preparation step S2 of the golden camellia tea essential oil, the steps include:

[0023] S21: Raw material pretreatment: The fermented Camellia chrysantha leaves are subjected to vacuum freeze drying to reduce the moisture content to ≤8%, and then subjected to low-temperature screw extrusion and expansion. After expansion, the leaves are crushed to 30-40 mesh to obtain Camellia chrysantha powder, which is evenly filled into an extraction kettle. The vacuum freeze drying temperature is -50°C to -30°C, the time is 18-28 hours, and the vacuum degree is 80±20Pa; the low-temperature screw extrusion and expansion temperature is 40-50°C, the pressure is 0-1MPa, and the time is 5-10 minutes.

[0024] S22: supercritical extraction: using food-grade CO2 as a solvent and anhydrous ethanol as an entraining agent, the Camellia nitidissima powder is extracted by dynamic circulation in a batch mode to obtain CO2 fluid containing essential oil components; wherein the purity of the food-grade CO2 is ≥ 99.9%, the volume of anhydrous ethanol accounts for 4-6% of the total volume of food-grade CO2; the batch mode is 35-45 min extraction followed by 5-15 min standing; the dynamic circulation extraction pressure is 20-35 MPa, the temperature is 20-50℃, the CO2 flow rate is 10-20 kg / h, and the time is 1-5 h;

[0025] S23: separation and purification: the CO2 fluid containing essential oil components is introduced into a two-stage separation system: the first stage separation removes wax and resin, the second stage separation collects the essential oil containing terpenes and flavonoids, the residual anhydrous ethanol is adsorbed, and the ethanol residue is reduced to <100 ppm by nitrogen blowing to obtain the Camellia nitidissima leaf essential oil; wherein the first stage separation pressure is 6-7 MPa, and the temperature is 35-40℃; the second stage separation pressure is 0.05-0.2 MPa, and the temperature is 4-6℃.

[0026] Specifically, the Camellia nitidissima leaf essential oil of the present application is first obtained by solid-state fermentation of Camellia nitidissima leaves with Camellia nitidissima fungus, which can direct degradation of macromolecular polyphenols into small molecule tea-brown and high-activity flavonoid glycosides (such as fuzhuanin A), significantly improving its antioxidant capacity and anti-inflammatory activity; further combined with supercritical CO2 extraction process, volatile terpenes (such as linalool) and heat-sensitive active substances are completely retained in a relatively low temperature environment, and anhydrous ethanol is used as an entraining agent to strengthen the dissolution of polar components, and finally a functional oil with natural floral fragrance and stability is obtained.

[0027] Preferably, the dynamic circulation extraction pressure is 30-35 MPa, the temperature is 40-50℃, the CO2 flow rate is 15-18 kg / h, and the time is 2.5-3.5 h.

[0028] In the second aspect, the present application provides the use of the skin-whitening composition containing Camellia nitidissima leaf essential oil in the first aspect in the preparation of cosmetics.

[0029] Preferably, the cosmetic is cosmetic water, emulsion, cream, mask, serum or spray, and the addition amount of the composition is 1%-5% of the total weight of the cosmetic.

[0030] In the third aspect, the present application provides an emulsion, which comprises the following raw materials by weight percentage: 1%-5% of the skin-whitening composition containing Camellia nitidissima leaf essential oil in the first aspect, 0.5%-1% of a thickening agent, 3%-5% of an emulsifying agent, 0.5%-5% of a humectant, 0.5%-3% of a preservative, and 0.01%-0.3% of a pH regulator, and the balance is deionized water.

[0031] Preferably, the humectant is at least one of glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, allantoin.

[0032] Preferably, the thickening agent is carbomer and / or xanthan gum.

[0033] Preferably, the emulsifier is at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetyl stearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, dimethicone, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, sucrose stearate.

[0034] Preferably, the pH regulator includes at least one of arginine, disodium ethylenediaminetetraacetate, tromethamine, disodium EDTA.

[0035] Preferably, the preservative includes at least one of 1,2-propanediol, 1,2-hexanediol, p-hydroxyacetophenone.

[0036] In a fourth aspect, the present application provides a preparation method of the emulsion in the third aspect, comprising the following steps:

[0037] (1) Mix the humectant, thickening agent and part of the pH regulator with water and stir, heat to 80-90℃, then homogenize, and after homogenization, keep warm for standby, to obtain a pre-prepared A phase;

[0038] (2) Mix the emulsifier, heat to 70-80℃, then homogenize, and after homogenization, keep warm for standby, to obtain a pre-prepared B phase;

[0039] (3) Mix the preservative, heat to 55-65℃ to melt, to obtain a pre-prepared C phase;

[0040] (4) Warm the pre-prepared A phase to 75-85℃, add the pre-prepared B phase and stir, then cool to 55-65℃, add the pre-prepared C phase and stir, then cool to below 45℃, add the skin-whitening composition containing the L. japonicus leaf essential oil and continue to stir, finally add the remaining pH regulator to adjust the pH to 5.5-7.0, stop stirring, discharge, and obtain the emulsion.

[0041] Compared with the prior art, the present application has the following beneficial effects:

[0042] The core innovation of this invention lies in the first application of microbial fermentation transformation and supercritical CO2 extraction technology to the preparation of golden camellia essential oil. Based on the synergistic mechanism of multi-source natural ingredients, a multi-effect skin rejuvenation composition that takes into account barrier repair, anti-inflammatory and redness-reducing, and antioxidant and brightening is constructed. The skin rejuvenation and brightening composition of the present invention is scientifically compounded with golden camellia essential oil and four functional ingredients: ascorbic acid polypeptide stabilizes the VC structure through the polypeptide chain, inhibits tyrosinase and activates collagen synthesis; oat β-glucan forms a water-retaining film through a unique β-(1→3,1→4) glycosidic bond, reduces transepidermal water loss and promotes barrier repair; camellia seed oil quickly penetrates with 78-86% oleic acid to replenish intercellular lipids, and synergizes with tea polyphenols to reduce lipid peroxidation; tiger nut oil enhances the expression of filaggrin by activating PPARγ receptors, consolidates the water-locking function and inhibits MMP-1 collagen degradation. The above five components form a synergistic enhancement system in the optimal ratio, which simultaneously blocks inflammatory roughness and dullness, breaking through the limitation of traditional products that only target dehydration-related roughness, and providing a comprehensive solution without hormone stimulation for sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a physical picture of golden camellia essential oil;

[0044] Figure 2 The figures are before and after comparison of the emulsions of Application Example 1 and Comparative Application Example 8; DETAILED DESCRIPTION

[0045] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0046] The sources of the raw materials used in the following examples and comparative examples are as follows:

[0047] Golden flower tea: The manufacturer is Vicky Biotechnology Co., Ltd.

[0048] Eurotium cristatum was purchased from China Industrial Culture Collection Center, strain number: CICC 2099.

[0049] Ascorbic acid peptide: The manufacturer is BIOHARVEST, the trade name is myoluminescent peptide;

[0050] Oat β-glucan: The manufacturer is Oriental Miaosen Biotechnology Co., Ltd., and the trade name is Miaokexiu;

[0051] Camellia seed oil: manufacturer is Vicky Biotechnology Co., Ltd.

[0052] Tiger nut oil: manufacturer is Vicky Biotechnology Co., Ltd.

[0053] Unless otherwise specified, other materials, reagents, etc. used in the Examples and Comparative Examples can be obtained from commercial sources.

[0054] Example 1

[0055] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 1.2:0.1:0.8:0.4:0.15, wherein the total weight of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts;

[0056] Specifically, the preparation method of golden camellia tea essential oil comprises the following steps:

[0057] S1. Solid-state fermentation of golden camellia tea and Eurotium cristatum is carried out, and the specific steps are as follows:

[0058] S11. Inoculate the spores of Eurotium cristatum onto PDA solid medium for cultivation at 28°C for 5 days, then add sterile water to wash the culture, and then evenly disperse it by vortexing and shaking to obtain spore solution, and adjust the spore solution concentration to 10 with sterile water. 7 PDA culture medium was purchased from Solarbio with the product number P8931.

[0059] S12: washing the fresh leaves of Camellia chrysantha, and drying the leaves at 60° C. to a moisture content of 10-20%;

[0060] S13: Evenly mixing the spore liquid and the dried golden camellia tea leaves, wherein the spore liquid content is 25 mL per 100 g of tea leaves, and performing solid fermentation under sealed conditions, stirring once every 4-5 days to prevent local overheating, to obtain the fermented golden camellia tea leaves; wherein the solid fermentation temperature is 29° C., the humidity is 83%, and the fermentation time is 11 days.

[0061] S2, subjecting the fermented golden camellia tea leaves to supercritical CO2 extraction, the specific steps being:

[0062] S21: Raw material pretreatment: The fermented Camellia chrysantha leaves are vacuum freeze-dried to a moisture content of ≤8%, then extruded and puffed by a low-temperature screw extruder. After puffing, the leaves are crushed to 30-40 mesh to obtain Camellia chrysantha powder, which is then evenly filled into an extraction kettle. The vacuum freeze-drying process is performed at a temperature of -40°C, a time of 24 hours, and a vacuum degree of 80 Pa; the low-temperature screw extrusion process is performed at a temperature of 45°C, a pressure of 0.5 MPa, and a time of 8 minutes.

[0063] S22: Supercritical extraction: Using food-grade CO2 as a solvent and anhydrous ethanol as an entrainer, the Camellia chrysantha powder is subjected to dynamic cyclic extraction in an intermittent mode to obtain a CO2 fluid containing essential oil components; wherein the purity of the food-grade CO2 is ≥99.9%, and the volume of anhydrous ethanol accounts for 5% of the total volume of the food-grade CO2; the intermittent mode is to extract for 40 minutes and then stand for 10 minutes; the dynamic cyclic extraction pressure is 32 MPa, the temperature is 45°C, the CO2 flow rate is 16 kg / h, and the time is 3 hours;

[0064] S23: Separation and refining: The CO2 fluid containing essential oil components enters a two-stage separation system: the first stage is to remove wax and resin, the second stage is to collect the essential oil body containing terpenes and flavonoids, and then use The molecular sieve adsorbed the residual anhydrous ethanol, and the ethanol residue was reduced to <100ppm by nitrogen purge, and the following was obtained: Figure 1 The golden camellia essential oil shown in FIG. 1 ; wherein, the pressure of the first separation is 6.5 MPa and the temperature is 38° C.; the pressure of the second separation is 0.1 MPa and the temperature is 5° C.

[0065] Example 2

[0066] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 1:0.15:0.5:0.5:0.2, wherein the total mass fraction of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts; the preparation method of the golden camellia essential oil is the same as that in Example 1.

[0067] Example 3

[0068] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 2:0.08:1:0.2:0.1, wherein the total mass fraction of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts; the preparation method of the golden camellia essential oil is the same as that in Example 1.

[0069] Example 4

[0070] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 0.5:0.5:3:1:0.5, wherein the total mass fraction of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts; the preparation method of the golden camellia essential oil is the same as that in Example 1.

[0071] Example 5

[0072] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 5:0.05:0.1:0.1:0.05, wherein the total mass fraction of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts; the preparation method of the golden camellia essential oil is the same as that in Example 1.

[0073] Example 6

[0074] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 0.1:1:5:5:5, wherein the total mass fraction of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts; the preparation method of the golden camellia essential oil is the same as that in Example 1.

[0075] Example 7

[0076] A skin-rejuvenating and brightening composition containing golden camellia essential oil, comprising golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil in a weight ratio of 10:0.01:0.01:0.01:0.01, wherein the total mass fraction of the golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil is 100 parts; the preparation method of the golden camellia essential oil is the same as that in Example 1.

[0077] Example 8

[0078] The difference between Example 8 and Example 1 is that in the preparation step S11 of the golden camellia tea essential oil in Example 8, 10 mL of spore liquid is added to every 100 g of tea leaves.

[0079] Example 9

[0080] The difference between Example 9 and Example 1 is that in the preparation step S11 of the golden camellia tea essential oil in Example 9, 30 mL of spore liquid is added to every 100 g of tea leaves.

[0081] Example 10

[0082] The difference between Example 10 and Example 1 is that in the preparation step S22 of golden camellia tea essential oil in Example 10, anhydrous ethanol is not added as an entrainer, and CO2 is used to make up for the missing amount.

[0083] Example 11

[0084] The difference between Example 11 and Example 1 is that in the preparation step S22 of golden camellia tea essential oil in Example 11, the dynamic circulation extraction pressure is 25 MPa, the temperature is 30°C, the CO2 flow rate is 20 kg / h, and the time is 5 hours.

[0085] Comparative Example 1

[0086] The difference between Comparative Example 1 and Example 1 is that: no golden camellia essential oil is added to the composition, and ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 0.1:0.8:0.4:0.15 are used to make up for the missing amount.

[0087] Comparative Example 2

[0088] The difference between Comparative Example 2 and Example 1 is that no ascorbic acid polypeptide is added to the composition, and the missing amount is made up by using golden camellia essential oil, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 1.2:0.8:0.4:0.15.

[0089] Comparative Example 3

[0090] The difference between Comparative Example 3 and Example 1 is that oat β-glucan is not added to the composition, and the missing amount is made up by using golden camellia essential oil, ascorbic acid polypeptide, camellia seed oil and tiger nut oil in a weight ratio of 1.2:0.1:0.4:0.15.

[0091] Comparative Example 4

[0092] The difference between Comparative Example 4 and Example 1 is that no camellia seed oil is added to the composition, and the missing amount is made up by using golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, and tiger nut oil in a weight ratio of 1.2:0.1:0.8:0.15.

[0093] Comparative Example 5

[0094] The difference between Comparative Example 5 and Example 1 is that: tiger nut oil is not added to the composition, and the missing amount is made up by using golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan and camellia seed oil in a weight ratio of 1.2:0.1:0.8:0.4:0.15.

[0095] Comparative Example 6

[0096] The difference between Comparative Example 6 and Example 1 is that the preparation method of golden camellia essential oil in the composition comprises the following steps:

[0097] S1: Wash the fresh leaves of Camellia chrysantha and dry them at 60°C until the moisture content is 10-20%.

[0098] S2. Subjecting the dried golden camellia tea leaves to supercritical CO2 extraction to obtain golden camellia tea essential oil, the specific steps are the same as those in Example 1.

[0099] Comparative Example 7

[0100] The difference between Comparative Example 7 and Example 1 is that the golden camellia essential oil is replaced with golden camellia extract;

[0101] The preparation method of the golden camellia tea extract comprises the following steps:

[0102] S1, solid fermentation of golden camellia tea and Eurotium cristatum, the specific steps are the same as those in Example 1, to obtain fermented golden camellia tea;

[0103] S2. The fermented golden camellia tea is subjected to a secondary ultrasonic vibration extraction method. The first extraction is performed with an ethanol aqueous solution with a mass concentration of 15%, and the solid-liquid ratio is 1:10. After extraction, the mixture is filtered, and the solid is taken out and further extracted. The second extraction is performed with pure water, and the solid-liquid ratio is 1:10. The two extracts are combined to obtain the golden camellia tea extract. The ultrasonic frequency of each extraction is 25 kHz, the temperature is 85°C, and the time is 90 min.

[0104] Application Example 1: Antioxidant Test of Composition

[0105] Experimental methods:

[0106] This application example explores the antioxidant effects of the compositions prepared in Examples 1-11 and Comparative Examples 1-7.

[0107] Experimental cells: The cell line used was human keratinocyte HaCaT (Shanghai Yaji Biotechnology Co., Ltd.), frozen at the 9th generation;

[0108] Test conditions: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%;

[0109] Cells were cultured and treated according to the groups and then tested as follows:

[0110] (1) Sample preparation: Dissolve the skin care composition samples of Examples 1-11 and Comparative Examples 1-7 in dimethyl sulfoxide (DMSO, final concentration ≤ 0.1%), adjust the concentration to 1% (v / v) by gradient dilution (DMEM culture medium containing 10% fetal bovine serum, v / v, 2105341, Gibco), and sterile filter through a 0.22 μm filter membrane to obtain the sample working solution of each group.

[0111] (2) The preserved human keratinocyte HaCaT cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well, and 100 μL of DMEM culture medium was added to each well for 24 h;

[0112] (3) Modeling and grouping processing:

[0113] The supernatant in the cell culture plate wells was discarded, and the cells were divided into blank control group, model group and sample group:

[0114] Model group: Hydrogen peroxide (H2O2, 100 μM) was added to each well of the culture plate to induce cells, and no composition sample working solution was added. The model group served as a positive control of oxidative damage to reflect the basal level of ROS induced by LPS.

[0115] Sample group: Hydrogen peroxide (H2O2, 100 μM) was added to each well of the culture plate to induce the cells, and then 100 μL of the composition sample working solution was added to each well for treatment, and the cells were placed in a cell culture incubator for co-culture for 24 h;

[0116] Blank control group: only resuscitated and cultured HaCaT cells, no LPS, no sample working solution, used to confirm whether H2O2 successfully induced ROS elevation.

[0117] (4) The ROS assay kit (chemiluminescence method) (ELISA, ml092661) was used to measure the ROS in the model group and the sample group. The instructions for the ROS assay kit (chemiluminescence method, DCFH-DA probe) were followed: no probe was added to the blank control group, 100 μL of 10 μM DCFH-DA working solution was added to each well of the sample group and the model group, and the cells were incubated at 37°C in the dark for 30 min. The probe solution was aspirated and the cells were washed three times with PBS to remove excess probe. The fluorescence intensity (A value) was immediately measured using a fluorescence microplate reader (excitation wavelength 502 nm, emission wavelength 530 nm). Each well was read three times and the average value was taken.

[0118] Reactive oxygen species (ROS) were determined using a reactive oxygen species (ROS) test kit (chemiluminescence method), and the fluorescence microplate reader was used to indirectly quantify the ROS level of each group of cells by measuring the fluorescence intensity (A value) of the DCFH-DA probe.

[0119] ROS level calculation method: Taking the blank control group as the benchmark (assuming its fluorescence intensity as 100%), the relative values ​​of ROS levels in the model group and the sample group were calculated using the formula:

[0120] Relative level of ROS 模型组 (%) = (A value 模型组 / A value 空白对照组 )×100%;

[0121] Relative level of ROS样品组 (%) = (A value 样品组 / A value 空白对照组 ) x 100%;

[0122] ROS improvement rate (%) = (ROS relative level 模型组 - ROS relative level 样品组 ) / ROS relative level 模型组 x 100%;

[0123] The higher the ROS improvement rate, the stronger the antioxidant capacity of the composition. The results are shown in Table 2.

[0124] Table 2 ROS improvement rate data of samples in each group

[0125] Group / Performance ROS improvement rate / % Example 1 72.3 Example 2 71.6 Example 3 72.0 Example 4 68.6 Example 5 69.4 Example 6 61.3 Example 7 62.7 Example 8 65.6 Example 9 70.8 Example 10 62.3 Example 11 64.5 Comparative Example 1 39.6 Comparative Example 2 45.1 Comparative Example 3 50.6 Comparative Example 4 49.0 Comparative Example 5 47.6 Comparative Example 6 42.8 Comparative Example 7 43.2

[0126] Test Example 2: Anti-inflammatory test of the composition

[0127] Sample: tender and brightening composition prepared from Examples 1-5 and Comparative Examples 1-7;

[0128] Test cell: The cell line used was human keratinocyte HaCaT (Shanghai Yaji Biological Technology Co., Ltd.), and the frozen generation number was the 9th generation;

[0129] Reagents and consumables:

[0130] DMSO (dimethyl sulfoxide, analytical pure, used for dissolving samples);

[0131] DMEM culture solution (containing 10% fetal bovine serum, 1% double antibody, v / v, Gibco, item number 2105341);

[0132] Capsaicin;

[0133] TNF-α ELISA detection kit (Shanghai Enzyme Link, item number ml061140);

[0134] 96-well cell culture plate, pipette, centrifuge, enzyme label instrument, etc.

[0135] Test conditions: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%;

[0136] According to the grouping, the cells were cultured and treated, and then tested. The test method is as follows:

[0137] (1) Sample preparation: Dissolve the skin care composition samples of Examples 1-11 and Comparative Examples 1-7 in dimethyl sulfoxide (DMSO, final concentration ≤ 0.1%), adjust the concentration to 1% (v / v) using a gradient dilution method (DMEM medium containing 10% fetal bovine serum, v / v, 2105341, Gibco), and sterile filter through a 0.22 μm filter membrane to obtain the sample working solution of each group;

[0138] (2) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well, 100 μL of culture medium was added to each well, and cultured for 24 h;

[0139] (3) Induction and sampling: Discard the supernatant in the wells and process according to group:

[0140] Control group: 100 μL of DMEM medium (containing 4.6 μg / mL capsaicin) was added to each well.

[0141] Sample group: 100 μL of DMEM medium containing 4.6 μg / mL capsaicin and 1 wt% sample working solution was added to each well.

[0142] The control group and the sample group were incubated in the incubator for 24 h.

[0143] (4) Detection of inflammatory factors: Follow the instructions of the ELISA kit (ml061140, Shanghai ELISA), place the ELISA plate in the microplate reader, select 450nm as the detection wavelength according to the TNF-α in the kit, and measure the absorbance (OD value) of each well. Use linear regression to fit the standard curve with the standard concentration as the X-axis and the OD value as the Y-axis. Substitute the sample OD value into the standard curve equation to calculate TNF-α (pg / mL). The specific formula is as follows:

[0144] TNF-α inhibition rate (%) = (1-TNF-α concentration 对照组 / TNF-α concentration 样品组 )×100%; see Table 2 for data.

[0145] Table 2 TNF-α inhibition rate data of each group of samples

[0146]

[0147]

[0148] As can be seen from Table 1-2, combined with the data of Example 1 and Example 2-7, the most preferred weight ratio range of golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil is (1-2): (0.08-0.15): (0.5-1): (0.2-0.5): (0.1-0.2), and the second preferred range is (0.5-5): (0.05-0.5): (0.1-3): (0.1-1): (0.05-0.5). Within the preferred range, the antioxidant and anti-inflammatory effects of the composition are at a relatively excellent level.

[0149] Combining the data of Example 1 and Examples 8-9, it can be seen that in the preparation step S1 of the golden camellia tea essential oil in Example 8, the amount of spore liquid added is relatively low, the fermentation degree of golden camellia tea is relatively low, and the contents of theabrownin and flavonoid glycosides in the golden camellia tea essential oil are relatively low. The amount of spore liquid added in Example 9 is 30 mL, and its antioxidant performance is similar to that of Example 1. Based on antioxidant, anti-inflammatory effects and cost considerations, in the preparation step S13 of the golden camellia tea essence, the spore liquid content per 100 g of tea is preferably 25-30 mL.

[0150] Combining the data of Example 1 and Example 10, it can be seen that in Example 10, no anhydrous ethanol was added to the supercritical CO2 extraction, and the antioxidant and anti-inflammatory effects of the composition were affected. This may be because the lack of anhydrous ethanol as an entrainer to enhance the dissolution of polar components resulted in incomplete dissolution of the active substance, affecting the antioxidant and anti-inflammatory effects.

[0151] Combining the data of Example 1 and Example 11, it can be seen that the parameters of dynamic circulation extraction are also one of the parameters that affect the antioxidant performance. The preferred parameters of dynamic circulation extraction are: pressure of 30-35 MPa, temperature of 40-50°C, CO2 flow rate of 15-18 kg / h, and time of 2.5-3.5 h.

[0152] Combining the data of Example 1 and Comparative Examples 1-5, it can be seen that the ingredients in the composition have a great influence on the antioxidant and anti-inflammatory effects. Golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil have a synergistic effect, and their combined use can improve the antioxidant and anti-inflammatory effects of the composition.

[0153] Combining the data of Example 1 and Comparative Examples 6-7, it can be seen that the antioxidant and anti-inflammatory effects of Comparative Examples 6 and 7 are lower than those of Example 1. This may be because the golden camellia essential oil of Comparative Example 6 is not directly subjected to supercritical CO2 extraction of golden camellia tea after solid fermentation, and the macromolecular effective components in the tea cannot be precipitated. The golden camellia extract of Comparative Example 7 is extracted by a mixed method of alcohol extraction and water extraction after solid fermentation of golden camellia tea, and its extraction rate of effective components is relatively low; this shows that the golden camellia essential oil of the present invention is first subjected to solid-state fermentation of golden camellia tea by golden flower fungus, and the macromolecular polyphenols are directionally degraded into small molecular theabrownins and flavonoid glycosides, significantly improving its antioxidant capacity and anti-inflammatory activity; further combined with the supercritical CO2 extraction process, volatile terpenes (such as linalool) and heat-sensitive active substances are completely retained in a relatively low temperature environment, while anhydrous ethanol is used as an entrainer to enhance the dissolution of polar components, and finally a functional oil with both natural floral fragrance and stability is obtained, which has good antioxidant and anti-inflammatory effects.

[0154] Application Example 1-11 and Comparative Application Example 1-7

[0155] The skin-rejuvenating and brightening compositions of Examples 1-11 and Comparative Examples 1-7 were added to the emulsion at a concentration of 4 wt %, respectively, to obtain the emulsions of Application Examples 1-7 and Comparative Application Examples 1-5. The formulas are shown in Table 3.

[0156] The preparation method of the emulsion of Application Examples 1-11 and Comparative Application Examples 1-7 comprises the following steps:

[0157] S1. Mix the humectant, thickener, and part of the pH adjuster (disodium EDTA) with water, heat to 85° C., and homogenize at 13,000 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated phase A.

[0158] (2) Mix the emulsifiers, heat to 75°C, and homogenize at 13,000 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain preformed phase B.

[0159] (3) Mix the preservatives and heat to 60°C to melt to obtain preformed phase C;

[0160] (4) The preformed phase A is heated to 80°C, and the preformed phase B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is cooled to 60°C, and the preformed phase C is added at a speed of 3000 rpm and stirred and mixed. Then, the temperature is cooled to below 45°C, and the soothing repair composition is added and stirring is continued for 8 minutes. Finally, the remaining pH adjuster (arginine) is added to adjust the pH to 6. Then, stirring is stopped and the material is discharged to obtain the emulsion.

[0161] Table 3 Emulsion formula of application examples 1-11 and comparative application examples 1-7

[0162]

[0163] Comparative Application Example 8

[0164] The emulsion of comparative application example 8 does not add the skin-rejuvenating and brightening composition containing golden camellia essential oil, and uses an equal amount of deionized water instead of the composition. The preparation method is the same as that of application example 1.

[0165] Test Example 3: Testing the softening, repairing, soothing, and brightening effects of lotions on the human body

[0166] 1. Experimental basis

[0167] Refer to the human efficacy evaluation test method in the "Technical Specifications for Safety of Cosmetics" (2015 edition) for implementation.

[0168] 2. Subject screening

[0169] Inclusion criteria: Asian female volunteers aged 45-60 years old who meet the following conditions:

[0170] a) Clinical assessment of skin laxity (grade I-III according to the clinical grading standard);

[0171] b) Self-reported history of skin sensitivity (confirmed by dermatologist evaluation);

[0172] c) No history of severe systemic disease or skin disease.

[0173] Exclusion criteria: pregnant / lactating women, people with severe allergies, and those who have participated in other clinical trials in the past 3 months.

[0174] Number setting: A total of 95 qualified volunteers were included and divided into 19 groups according to the random number table method, with 5 people in each group.

[0175] 3. Sample application method

[0176] Test samples: emulsions prepared in Application Examples 1-11 and Comparative Application Examples 1-8 (double-blind numbering), wherein the emulsion prepared in Comparative Application Example 8 was the blank control group, and the remaining application examples and comparative application examples were sample groups.

[0177] Usage: After cleansing every morning and evening, volunteers take 1mL of the sample and apply it evenly on the entire face, gently massaging until completely absorbed.

[0178] 4. Testing cycle and process

[0179] Test period: 14 days (D0-D 14 ).

[0180] Visit time points: D0 (baseline period), D 14 , conduct instrument testing.

[0181] Preparation before testing:

[0182] a) After the visit, the subjects cleansed their faces with a uniform non-irritating cleansing product;

[0183] b) Keep the specimen in a constant temperature and humidity environment (temperature 21±1°C, humidity 50±10%) for 30 minutes;

[0184] c) Keep your eyes closed and relaxed during the test to avoid interference from facial expressions and movements.

[0185] The facial skin condition was measured 14 days after product use to comprehensively evaluate the firming and anti-wrinkle efficacy of the test product. The specific test parameters and equipment used in the experiment are as follows:

[0186] The researchers used a Corneometer skin moisture test probe to measure the skin moisture content of the cheekbones; a Colorimeter CL440 probe to detect the facial brightness L* value. The volunteers' faces were photographed using Visia-7, and the red zone images were analyzed to obtain the a* value; a skin moisture loss test probe ( The transepidermal water loss (TEWL) of the zygomatic bone was measured using the TM Hex. The SEr index of the zygomatic skin was also measured using a VC20. On the volunteers' first visit, the initial value was measured before application. Then, after applying the sample to the entire face and sitting for 30 minutes, the value was measured 30 minutes after application.

[0187] Skin moisturizing ability is represented by the improvement of skin moisture content, skin brightening ability is represented by the improvement of L* value, skin soothing ability is represented by the improvement of a* value, skin barrier repair ability is represented by the improvement of TEWL value, and skin tenderness ability is represented by the improvement of skin roughness index SEr. The formula is as follows:

[0188] Skin moisture content improvement rate (%) = | (skin moisture content 使用后 -Skin moisture content 使用前 )| / Skin moisture content 使用前 ×100%;

[0189] Improvement in facial brightness L* value (%) = |(Facial brightness L* value 使用后 -Facial brightness L* value 使用前 )| / Facial brightness L* value 使用前 ×100%;

[0190] a* value improvement (%) = |(a* value 使用后 -a* value 使用前 )| / a*value 使用前 ×100%;

[0191] TEWL value improvement rate (%) = | (TEWL value 使用后 -TEWL value 使用前 )| / TEWL value 使用前 ×100%;

[0192] Skin roughness index SEr improvement rate (%) = | (SEr 使用后 -SEr 使用前 )| / SEr 使用前 ×100%; the calculation results are shown in Table 4.

[0193] Table 4 Human test results of each group of emulsions

[0194]

[0195]

[0196] Depend on Figure 2 It can be seen that the facial brightness, cheek redness and cheekbone skin roughness of the emulsion of Application Example 1 after 14 days of use are significantly improved compared with the emulsion of Comparative Application Example 8, which shows that the composition of the present invention achieves soothing, repairing, brightening and skin rejuvenation effects.

[0197] From the data of Application Example 1 and Comparative Application Examples 1-5 in Table 4, it can be seen that the skin moisture content, L* value, a* value, TEWL value and SEr of the emulsion in Application Example 1, which is a compound of five ingredients, namely, golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil, are significantly increased, indicating that the five ingredients, namely, golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil and tiger nut oil, have a synergistic effect, and the prepared emulsion has good soothing, repairing, brightening and skin rejuvenation effects.

[0198] Combining the data of Application Example 1 and Application Examples 2-7, it can be seen that when the weight ratio of Camellia chrysantha honey, dihydroavenate alkaloids, Myrtlesia serrata extract and Schisandra chinensis extract is (1-2): (0.08-0.15): (0.5-1): (0.2-0.5): (0.1-0.2), the emulsion has better soothing, repairing, brightening and skin rejuvenation effects.

[0199] Combining the data of Application Example 1 and Comparative Application Examples 6-7, it can be seen that Comparative Application Examples 6-7 lack the steps of solid fermentation of golden camellia tea and extraction by supercritical CO2 extraction, and the performance of its emulsion is lower than that of Application Example 1. This shows that the golden camellia tea essential oil of the present invention is a synergistic application of microbial fermentation transformation and supercritical CO2 extraction technology to the preparation of golden camellia tea essential oil, which directionally degrades large molecular polyphenols into small molecular theabrownins and flavonoid glycosides, significantly improving its antioxidant capacity and anti-inflammatory activity. At the same time, combined with the supercritical CO2 extraction process, volatile terpenes (such as linalool) and thermosensitive active substances are completely retained in a relatively low temperature environment, and anhydrous ethanol is used as an entrainer to enhance the dissolution of polar components, further improving the performance of the product.

[0200] In summary, the present invention's five components, including golden camellia essential oil, ascorbic acid polypeptide, oat β-glucan, camellia seed oil, and tiger nut oil, form a synergistic system in an optimal ratio, simultaneously blocking inflammatory roughness and dullness. This overcomes the limitation of traditional products that only target dehydration-related roughness, providing a comprehensive, hormone-free solution for sensitive skin. Furthermore, golden camellia essential oil is extracted through a combination of microbial fermentation and supercritical CO2 extraction, providing excellent antioxidant and anti-inflammatory effects.

[0201] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A skin-rejuvenating and brightening composition containing golden camellia essential oil, characterized in that: The invention comprises the following components: golden camellia essential oil, ascorbic acid polypeptide, oat beta-glucan, camellia seed oil and tiger nut oil, wherein the weight ratio of the golden camellia essential oil, ascorbic acid polypeptide, oat beta-glucan, camellia seed oil and tiger nut oil is (0.1-10): (0.01-1): (0.01-5): (0.01-5): (0.01-5); The method for preparing the golden camellia tea essential oil comprises the following steps: S1, solid fermentation of Camellia chrysantha and Eurotium cristatum; S2. Extracting the fermented golden camellia tea leaves with supercritical CO2 to obtain the golden camellia tea essential oil.

2. The skin-rejuvenating and brightening composition containing golden camellia essential oil according to claim 1, wherein: The weight ratio of the golden camellia essential oil, ascorbic acid polypeptide, oat beta-glucan, camellia seed oil and tiger nut oil is (0.5-5): (0.05-0.5): (0.1-3): (0.1-1): (0.05-0.5).

3. The skin-rejuvenating and brightening composition containing golden camellia essential oil according to claim 1, wherein: The weight ratio of the golden camellia essential oil, ascorbic acid polypeptide, oat beta-glucan, camellia seed oil and tiger nut oil is (1-2): (0.08-0.15): (0.5-1): (0.2-0.5): (0.1-0.2).

4. The skin-rejuvenating and brightening composition containing golden camellia essential oil according to claim 1, wherein: In the preparation step S1 of the golden camellia tea essential oil, the steps specifically include: S11, inoculate the cristatum onto PDA solid medium for cultivation, add sterile water to wash the culture, and then evenly disperse it by vortexing and shaking to obtain spore liquid, and adjust the spore liquid concentration to 10 with sterile water. 6 -10 8 / mL; S12: Wash the fresh leaves of Camellia chrysantha and dry them until the moisture content is 10-20%; S13: Evenly mixing the spore liquid and the dried golden camellia tea leaves, adding 10-30 mL of the spore liquid to every 100 g of tea leaves, and performing solid fermentation under closed conditions to obtain the fermented golden camellia tea leaves; wherein the solid fermentation temperature is 26-32° C., the humidity is 80-85%, and the fermentation time is 8-20 days.

5. The skin-rejuvenating and brightening composition containing golden camellia essential oil according to claim 1, wherein: In the preparation step S2 of the golden camellia tea essential oil, the steps specifically include: S21: Raw material pretreatment: The fermented Camellia chrysantha leaves are subjected to vacuum freeze drying to reduce the moisture content to ≤8%, and then subjected to low-temperature screw extrusion and expansion. After expansion, the leaves are crushed to 30-40 mesh to obtain Camellia chrysantha powder, which is evenly filled into an extraction kettle. The vacuum freeze drying temperature is -50°C to -30°C, the time is 18-28 hours, and the vacuum degree is 80±20Pa; the low-temperature screw extrusion and expansion temperature is 40-50°C, the pressure is 0-1MPa, and the time is 5-10 minutes. S22: Supercritical extraction: Using food-grade CO2 as a solvent and anhydrous ethanol as an entrainer, the Camellia chrysantha powder is subjected to dynamic cyclic extraction in an intermittent mode to obtain a CO2 fluid containing essential oil components; wherein the purity of the food-grade CO2 is ≥99.9%, and the volume of anhydrous ethanol accounts for 4-6% of the total volume of the food-grade CO2; the intermittent mode is to extract for 35-45 minutes and then stand for 5-15 minutes; the dynamic cyclic extraction pressure is 20-35MPa, the temperature is 20-50°C, the CO2 flow rate is 10-20kg / h, and the extraction time is 1-5 hours; S23: Separation and refining: The CO2 fluid containing essential oil components enters a two-stage separation system: the first-stage separation removes waxes and resins, and the second-stage separation collects the essential oil body containing terpenes and flavonoids, and then adsorbs the residual anhydrous ethanol, and reduces the residual ethanol to <100ppm by nitrogen purge to obtain the golden camellia essential oil; wherein, the pressure of the first-stage separation is 6-7MPa and the temperature is 35-40℃; the pressure of the second-stage separation is 0.05-0.2MPa and the temperature is 4-6℃.

6. Use of the skin-rejuvenating and brightening composition containing golden camellia essential oil according to any one of claims 1 to 5 in the preparation of cosmetics.

7. Use of the skin-rejuvenating and brightening composition containing golden camellia essential oil according to claim 6 in the preparation of cosmetics, characterized in that: The cosmetic is lotion, emulsion, cream, facial mask, essence or spray, and the added amount of the composition is 1%-5% of the total weight of the cosmetic.

8. An emulsion, characterized in that The composition comprises the following raw materials in percentage by weight: 1%-5% of the skin-rejuvenating and brightening composition containing golden camellia tea essential oil according to any one of claims 1 to 5, 0.5%-1% of a thickener, 3%-5% of an emulsifier, 0.5%-5% of a moisturizer, 0.5%-3% of a preservative, and 0.01%-0.3% of a pH regulator, with the balance being deionized water.

9. The emulsion according to claim 8, wherein The raw material is selected from at least one of (I) to (V): (I) The moisturizing agent is at least one of glycerin, D-panthenol, vitamin B5, 1,3-butylene glycol, 1,2-hexanediol, 1,3-propylene glycol, sodium hyaluronate, Tremella fuciformis polysaccharide, trehalose, betaine, and allantoin; (II) the thickener is carbomer and / or xanthan gum; (III) the emulsifier is at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; (IV) The pH regulator includes at least one of arginine, disodium EDTA, tromethamine, and disodium EDTA. (V) The preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone.

10. The method for preparing the emulsion according to claim 8, characterized in that: The following steps are involved: (1) Mixing a moisturizer, a thickener, and part of a pH adjuster with water, stirring, heating to 80-90° C., and homogenizing. After homogenization, keep the mixture warm for later use to obtain a prefabricated phase A; (2) Mix the emulsifiers, heat to 70-80°C, and homogenize. After homogenization, keep the mixture warm for later use to obtain preformed phase B; (3) Mix the preservatives and heat to 55-65°C to melt to obtain preformed phase C; (4) The preformed phase A is heated to 75-85°C, the preformed phase B is added, and the mixture is stirred and mixed. The temperature is then lowered to 55-65°C, and the preformed phase C is added and stirred and mixed. The temperature is then lowered to below 45°C, and the skin-rejuvenating and brightening composition containing golden camellia essential oil is added and the stirring is continued. Finally, the remaining pH adjuster is added to adjust the pH to 5.5-7.0, and the stirring is stopped. The material is discharged to obtain the emulsion.

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