A skin-smoothing and lightening composition containing camellia leaf oil and a preparation method and application thereof

Abstract

This invention relates to a skin-brightening composition containing *Tea laurentii* essential oil, its preparation method, and its application. The composition comprises the following components: *Tea laurentii* essential oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil. The weight ratio of *Tea laurentii* essential oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil is (0.1-10):(0.01-1):(0.01-5):(0.01-5):(0.01-5). The *Tea laurentii* essential oil is obtained by fermenting *Tea laurentii* tea leaves with *Aspergillus cristatus* followed by supercritical CO2 processing. 2 The composition obtained by extraction; the components of the present invention have synergistic effects, simultaneously blocking inflammatory roughness and dullness, breaking through the limitation of traditional products that only target dehydrated roughness, and providing a comprehensive solution for sensitive skin without hormone stimulation.

Description

A skin-nourishing and brightening composition containing golden tea leaf essential oil, its preparation method and application Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a skin-brightening and skin-rejuvenating composition containing golden tea leaf essential oil, its preparation method, and its application. Background Technology

[0002] Current skincare products targeting rough and dull skin primarily address dryness-related roughness, but this is insufficient. Inflammation-induced roughness is more severe and harder to resolve, and it also contributes to dullness. Inflammation causes a yellowish complexion, while roughness and an uneven stratum corneum affect light reflection, leading to a dull appearance. Most skincare products focus on dehydration-related roughness (e.g., adding hyaluronic acid and ceramides for hydration), but they cannot block the inflammatory cascade. Synthetic anti-inflammatory ingredients (such as steroid creams) can temporarily suppress erythema, but they exacerbate barrier damage and the risk of pigmentation. Traditional plant oils (such as common camellia oil), due to their large molecular weight and poor permeability, are unable to inhibit deep inflammatory signal transduction, leading to recurring inflammatory roughness and persistently worsening dullness. Furthermore, conventional extraction processes (such as hot pressing) easily destroy active ingredients, causing oil oxidation and rancidity, requiring the addition of chemical preservatives (such as phenoxyethanol), which further induce irritation. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the prior art and provide a skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil, its preparation method, and its application.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0005] In a first aspect, the present invention provides a skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil, comprising the following components: golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil, wherein the weight ratio of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil is (0.1-10):(0.01-1):(0.01-5):(0.01-5):(0.01-5);

[0006] The preparation method of the golden flower tea essential oil includes the following steps:

[0007] S1. Solid-state fermentation of golden chrysanthemum tea leaves with *Eurotium cristatum*.

[0008] S2. The fermented golden chrysanthemum tea leaves are subjected to supercritical CO2 extraction to obtain the golden chrysanthemum tea essential oil.

[0009] The functions of each component in the skin-rejuvenating and brightening composition of the present invention are as follows:

[0010] Golden chrysanthemum tea oil is extracted from golden chrysanthemum tea leaves through solid-state fermentation using *Eurotium cristatum*. Through microbial metabolic transformation, the large polyphenol molecules in the tea are degraded into smaller theabrownins and flavonoids (such as fuzhuanin A), significantly enhancing antioxidant and anti-inflammatory activity. Supercritical CO2 extraction preserves volatile terpenes (such as linalool), imparting a natural floral aroma and preventing oil rancidity. Golden chrysanthemum tea oil reduces erythema and abnormal keratinization by inhibiting the TNF-α inflammatory pathway, while simultaneously scavenging free radicals to block pigmentation, thus improving inflammatory roughness and dullness, making it suitable for sensitive skin repair.

[0011] Ascorbic acid peptides, as a stable derivative of vitamin C, integrate L-ascorbic acid with a polypeptide chain in their structure, overcoming the shortcomings of traditional vitamin C which is easily oxidized and inactivated. They can inhibit tyrosinase activity, block the melanin synthesis pathway, and brighten skin tone. Secondly, they can activate collagen synthesis in fibroblasts, improving skin texture and elasticity. Their small molecule properties support transdermal absorption, are gentle and non-irritating, suitable for repairing dullness and acne scars caused by photoaging, and pose no risk of photosensitivity.

[0012] Oat β-glucan, a natural polysaccharide extracted from oat bran, possesses a unique β-(1→3,1→4) glycosidic bond linear structure, forming a highly viscous hydrating film. It forms a breathable, moisture-retaining layer on the skin surface, reducing transepidermal water loss (TEWL); simultaneously, it activates macrophages to release epidermal growth factor, promoting keratinocyte migration and barrier repair. Its anti-inflammatory properties can relieve neurogenic itching, making it suitable for deep moisturizing and repairing damaged barriers in dry and sensitive skin, especially for dehydration and roughness caused by over-cleansing.

[0013] Camellia seed oil is a cold-pressed oil derived from the seeds of the camellia (Camellia oleifera) plant. It is rich in oleic acid (78-86%) and squalene, with a fatty acid ratio highly similar to human sebum. It rapidly penetrates to replenish intercellular lipids and repair the lipid bilayer structure; oleic acid softens hardened keratin and improves uneven skin texture. Furthermore, the tea polyphenols it contains synergistic antioxidant effects, reducing UV-induced lipid peroxidation. Suitable for dry, chapped, and aging-related roughness, it can be used as a massage oil to enhance skin suppleness.

[0014] Tiger nut oil is a plant oil extracted from tiger nuts (Cyperus esculentus). Its core active ingredients are tocopherol (vitamin E) and oleic acid-linoleic acid complex, exhibiting significant antioxidant capabilities. Its small-molecule lipids can penetrate deep into the stratum corneum, activating peroxisome proliferator-activated receptor (PPARγ), promoting the expression of barrier proteins (such as filaggrin), and strengthening water-locking function; simultaneously, it inhibits matrix metalloproteinase-1 (MMP-1) activity, delaying collagen degradation. It is suitable for repairing dullness and photodamage caused by oxidative stress and can synergistically enhance the photoprotective effect of sunscreen products.

[0015] Preferably, the weight ratio of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil is (0.5-5):(0.05-0.5):(0.1-3):(0.1-1):(0.05-0.5).

[0016] More preferably, the weight ratio of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil is (1-2):(0.08-0.15):(0.5-1):(0.2-0.5):(0.1-0.2).

[0017] Preferably, step S1 of preparing the golden chrysanthemum tea essential oil specifically includes:

[0018] S11. Inoculate *Eurotium cristatum* onto PDA solid medium and culture. After culturing, add sterile water to wash off the culture, then disperse it evenly by vortexing and shaking to obtain a spore solution. Adjust the spore solution concentration to 10% with sterile water. 6 -10 8 cells / mL;

[0019] S12: Wash the fresh leaves of Camellia chrysantha and dry them until the moisture content is 10-20%;

[0020] S13: Mix the spore liquid and the dried golden chrysanthemum tea leaves evenly, add 10-30mL of spore liquid to every 100g of tea leaves, and carry out solid-state fermentation under sealed conditions to obtain the fermented golden chrysanthemum tea leaves; wherein, the solid-state fermentation temperature is 26-32℃, the humidity is 80-85%, and the time is 8-20 days.

[0021] Preferably, the spore liquid content in each 100g of tea is 20-30mL, and the solid-state fermentation temperature is 28-30℃ and the time is 10-12 days.

[0022] Preferably, step S2 in the preparation of the golden chrysanthemum tea essential oil specifically includes:

[0023] S21: Raw material pretreatment: Fermented golden chrysanthemum tea leaves are freeze-dried under vacuum to reduce moisture content to ≤8%, then extruded and puffed under low temperature. After puffing, the leaves are pulverized to 30-40 mesh to obtain golden chrysanthemum tea powder. The golden chrysanthemum tea powder is then uniformly filled into the extraction vessel. The vacuum freeze-drying temperature is -50℃ to -30℃, the time is 18-28 hours, and the vacuum degree is 80±20 Pa. The low temperature screw extrusion puffing temperature is 40-50℃, the pressure is 0-1 MPa, and the time is 5-10 minutes.

[0024] S22: Supercritical extraction: Using food-grade CO2 as solvent and anhydrous ethanol as entrainer, Camellia chrysantha powder is subjected to dynamic cyclic extraction in an intermittent mode to obtain a CO2 fluid containing essential oil components; wherein, the purity of the food-grade CO2 is ≥99.9%, and the volume of anhydrous ethanol accounts for 4-6% of the total volume of the food-grade CO2; the intermittent mode is extraction for 35-45 min followed by standing for 5-15 min; the dynamic cyclic extraction pressure is 20-35 MPa, the temperature is 20-50℃, the CO2 flow rate is 10-20 kg / h, and the time is 1-5 h;

[0025] S23: Separation and Refining: The CO2 fluid containing essential oil components is introduced into a two-stage separation system: the first stage separates the wax and resin, the second stage separates the essential oil bulk containing terpenes and flavonoids, then adsorbs the residual anhydrous ethanol, and the ethanol residue is reduced to <100 ppm by nitrogen purging to obtain the golden tea essential oil; wherein, the pressure of the first stage separation is 6-7 MPa and the temperature is 35-40℃; the pressure of the second stage separation is 0.05-0.2 MPa and the temperature is 4-6℃.

[0026] Specifically, the Golden Flower Tea Oil of the present invention first undergoes solid-state fermentation of Golden Flower Tea leaves by Golden Flower Fungus, which directionally degrades macromolecular polyphenols into small molecule theaflavins and highly active flavonoid glycosides (such as fuzhuanin A), significantly enhancing its antioxidant capacity and anti-inflammatory activity. Furthermore, it is combined with supercritical CO2 extraction technology to completely preserve volatile terpenes (such as linalool) and heat-sensitive active substances under relatively low temperature conditions. At the same time, anhydrous ethanol is used as an entrainer to enhance the dissolution of polar components, ultimately obtaining a functional oil that combines natural floral fragrance and stability.

[0027] Preferably, the dynamic circulation extraction pressure is 30-35 MPa, the temperature is 40-50℃, the CO2 flow rate is 15-18 kg / h, and the time is 2.5-3.5 h.

[0028] In a second aspect, the present invention provides the application of the skin-rejuvenating and brightening composition containing golden tea leaf essential oil in the first aspect in the preparation of cosmetics.

[0029] Preferably, the cosmetic is a toner, lotion, cream, mask, serum, or spray, and the amount of the composition added is 1%-5% of the total weight of the cosmetic.

[0030] Thirdly, the present invention provides an emulsion comprising the following ingredients by weight percentage: 1%-5% of the skin-brightening composition containing golden tea leaf essential oil as described in the first aspect, 0.5%-1% thickener, 3%-5% emulsifier, 0.5%-5% moisturizer, 0.5%-3% preservative, and 0.01%-0.3% pH adjuster, with the balance being deionized water.

[0031] Preferably, the moisturizer is at least one of glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, and allantoin.

[0032] Preferably, the thickener is carbomer and / or xanthan gum.

[0033] Preferably, the emulsifier is at least one selected from the following: caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0034] Preferably, the pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA.

[0035] Preferably, the preservative includes at least one of 1,2-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0036] Fourthly, the present invention provides a method for preparing the emulsion of the third aspect, comprising the following steps:

[0037] (1) Mix the humectant, thickener and part of the pH adjuster with water and stir. Heat to 80-90℃ and homogenize. After homogenization, keep warm for later use to obtain the pre-prepared phase A.

[0038] (2) Mix the emulsifier, heat to 70-80℃, homogenize, and keep warm after homogenization to obtain the pre-made phase B;

[0039] (3) Mix the preservatives and heat them to 55-65℃ to melt them, thus obtaining the pre-prepared C phase;

[0040] (4) Heat the pre-prepared phase A to 75-85℃, add the pre-prepared phase B, stir and mix, then cool down to 55-65℃, add the pre-prepared phase C and stir and mix, then cool down to below 45℃, add the skin-brightening composition containing golden tea essential oil and continue stirring, finally add the remaining pH adjuster to adjust the pH to 5.5-7.0, then stop stirring, discharge the material, and obtain the emulsion.

[0041] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0042] The core innovation of this invention lies in the first-time application of microbial fermentation and supercritical CO2 extraction technology to the preparation of Camellia sinensis tea essential oil, and the construction of a multi-effect skin-rejuvenating composition based on the synergistic mechanism of multiple natural ingredients, which takes into account barrier repair, anti-inflammatory and redness reduction, and antioxidant brightening. In the skin-rejuvenating and brightening composition of this invention, Camellia sinensis tea essential oil is scientifically compounded with four active ingredients: ascorbic acid peptides stabilize the VC structure through polypeptide chains, inhibit tyrosinase, and activate collagen synthesis; oat β-glucan forms a water-retaining film through unique β-(1→3,1→4) glycosidic bonds, reducing transepidermal water loss and promoting barrier repair; camellia seed oil rapidly penetrates and replenishes intercellular lipids with 78-86% oleic acid, synergistically reducing lipid peroxidation with tea polyphenols; and tiger nut oil enhances filaggrin expression by activating PPARγ receptors, consolidating water-locking function and inhibiting MMP-1 collagen degradation. The five components mentioned above form a synergistic system when used in an optimized ratio, simultaneously blocking inflammatory roughness and dullness. This breaks through the limitations of traditional products that only target dehydrated roughness, providing a comprehensive solution for sensitive skin without hormone stimulation. Attached Figure Description

[0043] Figure 1 shows a picture of the actual product of Golden Flower Tea Essential Oil;

[0044] Figure 2 shows a comparison of the emulsion before and after use in Application Example 1 and Comparative Application Example 8. Detailed Implementation

[0045] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0046] The sources of the raw materials used in the following examples and comparative examples are as follows:

[0047] Golden Flower Tea: Manufacturer is Viki Biotechnology Co., Ltd.

[0048] *Aspergillus cristatus*: purchased from China Industrial Microbial Culture Collection Center, strain number: CICC 2099.

[0049] Ascorbic acid peptide: Manufacturer is BIOHARVEST Pharmaceuticals, trade name is Myo-Light Peptide;

[0050] Oat β-glucan: Manufacturer is Dongfang Miaosen Biotechnology Co., Ltd., and the product name is Miaokexiu;

[0051] Camellia seed oil: manufactured by Viki Biotechnology Co., Ltd.

[0052] Tiger nut oil: manufactured by Vichi Biotechnology Co., Ltd.

[0053] Unless otherwise specified, all other materials, reagents, etc. used in the examples and comparative examples are commercially available.

[0054] Example 1

[0055] A skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 1.2:0.1:0.8:0.4:0.15, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts;

[0056] Specifically, the preparation method of the golden flower tea essential oil includes the following steps:

[0057] S1. Solid-state fermentation of golden chrysanthemum tea leaves with *Eurotium cristatum* is carried out. The specific steps are as follows:

[0058] S11. Inoculate *Eurotium cristatum* onto PDA solid medium and culture at 28°C for 5 days. Then, add sterile water to wash off the culture, and disperse it evenly by vortexing and shaking to obtain a spore solution. Adjust the spore solution concentration to 10% with sterile water. 7 The PDA medium was purchased from Solarbio, catalog number P8931.

[0059] S12: Wash the fresh leaves of Camellia chrysantha and dry them at 60℃ until the moisture content is 10-20%;

[0060] S13: Mix the spore liquid and dried golden chrysanthemum tea leaves evenly, with a spore liquid content of 25 mL per 100 g of tea leaves. Carry out solid fermentation under sealed conditions, turning the mixture every 4-5 days to prevent local overheating, to obtain the fermented golden chrysanthemum tea leaves. The solid fermentation temperature is 29℃, the humidity is 83%, and the time is 11 days.

[0061] S2. The fermented golden chrysanthemum tea leaves are subjected to supercritical CO2 extraction. The specific steps are as follows:

[0062] S21: Raw material pretreatment: Fermented golden chrysanthemum tea leaves are freeze-dried under vacuum to reduce moisture content to ≤8%, then extruded and puffed using a low-temperature screw extruder. After puffing, the leaves are pulverized to 30-40 mesh to obtain golden chrysanthemum tea powder. The golden chrysanthemum tea powder is then evenly filled into the extraction vessel. The vacuum freeze-drying temperature is -40℃, the time is 24h, and the vacuum degree is 80Pa. The low-temperature screw extrusion puffing temperature is 45℃, the pressure is 0.5MPa, and the time is 8min.

[0063] S22: Supercritical extraction: Using food-grade CO2 as solvent and anhydrous ethanol as entrainer, Camellia chrysantha powder is subjected to dynamic cyclic extraction in intermittent mode to obtain a CO2 fluid containing essential oil components; wherein, the purity of the food-grade CO2 is ≥99.9%, and the volume of anhydrous ethanol accounts for 5% of the total volume of food-grade CO2; the intermittent mode is extraction for 40 min followed by standing for 10 min; the dynamic cyclic extraction pressure is 32 MPa, the temperature is 45℃, the CO2 flow rate is 16 kg / h, and the time is 3 h;

[0064] S23: Separation and Refining: The CO2 fluid containing essential oil components is introduced into a two-stage separation system: the first stage separates the wax and resin, the second stage separates the essential oil bulk containing terpenes and flavonoids, the residual anhydrous ethanol is then adsorbed with a 3Å molecular sieve, and the ethanol residue is reduced to <100 ppm by nitrogen purging, to obtain the golden tea essential oil shown in Figure 1; wherein, the pressure of the first stage separation is 6.5 MPa and the temperature is 38℃; the pressure of the second stage separation is 0.1 MPa and the temperature is 5℃.

[0065] Example 2

[0066] A skin-brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 1:0.15:0.5:0.5:0.2, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts; the preparation method of the golden chrysanthemum tea essential oil is the same as in Example 1.

[0067] Example 3

[0068] A skin-brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 2:0.08:1:0.2:0.1, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts; the preparation method of the golden chrysanthemum tea essential oil is the same as in Example 1.

[0069] Example 4

[0070] A skin-brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 0.5:0.5:3:1:0.5, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts; the preparation method of the golden chrysanthemum tea essential oil is the same as in Example 1.

[0071] Example 5

[0072] A skin-brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 5:0.05:0.1:0.1:0.05, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts; the preparation method of the golden chrysanthemum tea essential oil is the same as in Example 1.

[0073] Example 6

[0074] A skin-brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 0.1:1:5:5:5, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts; the preparation method of the golden chrysanthemum tea essential oil is the same as in Example 1.

[0075] Example 7

[0076] A skin-brightening composition containing golden chrysanthemum tea essential oil comprises golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 10:0.01:0.01:0.01:0.01, wherein the total mass parts of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil are 100 parts; the preparation method of the golden chrysanthemum tea essential oil is the same as in Example 1.

[0077] Example 8

[0078] The difference between Example 8 and Example 1 is that in step S11 of the preparation of golden chrysanthemum tea essential oil in Example 8, 10 mL of spore liquid is added for every 100 g of tea leaves.

[0079] Example 9

[0080] The difference between Example 9 and Example 1 is that in step S11 of the preparation of golden chrysanthemum tea essential oil in Example 9, 30 mL of spore liquid is added for every 100 g of tea leaves.

[0081] Example 10

[0082] The difference between Example 10 and Example 1 is that in step S22 of the preparation of golden chrysanthemum tea essential oil in Example 10, anhydrous ethanol is not added as an entrainer, and CO2 is used to make up for the missing amount.

[0083] Example 11

[0084] The difference between Example 11 and Example 1 is that in step S22 of the preparation of golden chrysanthemum tea essential oil in Example 11, the dynamic circulation extraction pressure is 25 MPa, the temperature is 30°C, the CO2 flow rate is 20 kg / h, and the time is 5 h.

[0085] Comparative Example 1

[0086] The difference between Comparative Example 1 and Example 1 is that: no golden chrysanthemum tea essential oil was added to the composition, and ascorbic acid peptides, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 0.1:0.8:0.4:0.15 were used to make up the missing amount.

[0087] Comparative Example 2

[0088] The difference between Comparative Example 2 and Example 1 is that ascorbic acid peptides are not added to the composition, and the missing amount is made up by using golden tea leaf essential oil, oat β-glucan, camellia seed oil and tiger nut oil in a weight ratio of 1.2:0.8:0.4:0.15.

[0089] Comparative Example 3

[0090] The difference between Comparative Example 3 and Example 1 is that oat β-glucan is not added to the composition, and the missing amount is made up by using golden tea leaf essential oil, ascorbic acid peptide, camellia seed oil and tiger nut oil in a weight ratio of 1.2:0.1:0.4:0.15.

[0091] Comparative Example 4

[0092] The difference between Comparative Example 4 and Example 1 is that camellia seed oil was not added to the composition, and golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan and tiger nut oil in a weight ratio of 1.2:0.1:0.8:0.15 were used to make up the missing amount.

[0093] Comparative Example 5

[0094] The difference between Comparative Example 5 and Example 1 is that tiger nut oil was not added to the composition, and the missing amount was made up by golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan and camellia seed oil in a weight ratio of 1.2:0.1:0.8:0.4:0.15.

[0095] Comparative Example 6

[0096] The difference between Comparative Example 6 and Example 1 is that the preparation method of the golden chrysanthemum tea essential oil in the composition includes the following steps:

[0097] S1: Wash the fresh leaves of Camellia chrysantha and dry them at 60℃ until the moisture content is 10-20%.

[0098] S2. The dried golden chrysanthemum tea leaves are subjected to supercritical CO2 extraction to obtain golden chrysanthemum tea essential oil. The specific steps are the same as in Example 1.

[0099] Comparative Example 7

[0100] The difference between Comparative Example 7 and Example 1 is that the golden chrysanthemum tea essential oil was replaced with golden chrysanthemum tea extract;

[0101] The preparation method of the golden flower tea extract includes the following steps:

[0102] S1. The golden flower tea leaves and *Eurotium cristatum* were subjected to solid-state fermentation. The specific steps were the same as in Example 1, and the fermented golden flower tea leaves were obtained.

[0103] S2. The fermented golden chrysanthemum tea leaves are extracted using a two-stage ultrasonic oscillation method. The first extraction is performed using a 15% ethanol aqueous solution with a material-to-liquid ratio of 1:10. After extraction, the mixture is filtered, and the solid is removed for further extraction. The second extraction is performed using pure water with a material-to-liquid ratio of 1:10. The extracts from the two extractions are combined to obtain the golden chrysanthemum tea leaf extract. The ultrasonic frequency for each extraction is 25kHz, the temperature is 85℃, and the time is 90min.

[0104] Application Example 1: Antioxidant Test of the Composition

[0105] Experimental methods:

[0106] This application example explores the antioxidant effects of the compositions prepared in Examples 1-11 and Comparative Examples 1-7.

[0107] Experimental cells: The cell line used was human keratinocyte HaCaT (Shanghai Yaji Biotechnology Co., Ltd.), which was cryopreserved to the 9th generation.

[0108] Experimental conditions: Incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%;

[0109] Cells were cultured and treated according to their groups, followed by testing. The testing methods are as follows:

[0110] (1) Sample preparation: Take the skin care composition samples of Examples 1-11 and Comparative Examples 1-7 respectively, dissolve them in dimethyl sulfoxide (DMSO, final concentration ≤0.1%), and adjust to a final concentration of 1% (v / v) by gradient dilution (DMEM medium, containing 10% fetal bovine serum, v / v, 2105341, Gibco). Filter aseptically through a 0.22 μm filter membrane for later use to obtain the working solution of each group.

[0111] (2) The preserved human keratinocyte HaCaT cell suspension was seeded into a 96-well cell culture plate at a density of 2000 cells / well, and 100 μL of LMEM culture medium was added to each well. The cells were cultured for 24 h.

[0112] (3) Modeling and grouping:

[0113] Discard the supernatant from the cell culture plate wells, and then divide the cells into three groups: blank control group, model group, and sample group.

[0114] Model group: Hydrogen peroxide (H2O2, 100 μM) was added to each well of the culture plate to induce cells, without adding the working solution of the composition sample. The model group served as a positive control for oxidative damage, reflecting the baseline level of ROS induced by LPS.

[0115] Sample group: Add hydrogen peroxide (H2O2, 100μM) to each well of the culture plate to induce cells, then add 100μL of the sample working solution of the composition and co-culture in a cell culture incubator for 24h.

[0116] Blank control group: consisting only of resuscitated and cultured HaCaT cells, without LPS or sample working solution, used to confirm whether H2O2 successfully induced an increase in ROS.

[0117] (4) The reactive oxygen species (ROS) assay kit (chemiluminescence method) (ELISA, ml092661) was used to determine the ROS in the model group and the sample group. The procedure was performed according to the instructions of the ROS assay kit (chemiluminescence method, DCFH-DA probe): no probe was added to the blank control group, and 100 μL of 10 μM DCFH-DA working solution was added to each well of the sample group and the model group. The mixture was incubated at 37°C in the dark for 30 min. The probe solution was discarded, and the sample group was washed three times with PBS to remove excess probe. The fluorescence intensity (A value) was immediately measured using a fluorescence microplate reader (excitation wavelength 502 nm, emission wavelength 530 nm). The average value was taken from three readings for each well.

[0118] The reactive oxygen species (ROS) assay was performed using a reactive oxygen species (ROS) assay kit (chemiluminescence method). The fluorescence microplate reader indirectly quantified the ROS level of each group of cells by measuring the fluorescence intensity (A value) of the DCFH-DA probe.

[0119] ROS level calculation method: Using the blank control group as a baseline (assuming its fluorescence intensity is 100%), the relative ROS levels of the model group and the sample group are calculated using the following formula:

[0120] ROS relative level 模型组 (%) = (A value) 模型组 / A value 空白对照组 ) × 100%;

[0121] ROS relative level 样品组 (%) = (A value) 样品组 / A value 空白对照组 ) × 100%;

[0122] ROS improvement rate (%) = (Relative level of ROS) 模型组 -Relative level of ROS 样品组 ) / ROS relative level 模型组 ×100%;

[0123] The higher the ROS improvement rate, the stronger the antioxidant capacity of the composition. The results are shown in Table 1.

[0124] Table 1. ROS improvement rate data for each group of samples.

[0125] Group / Performance ROS Improvement Rate / % Example 1 72.3 Example 2 71.6 Example 3 72.0 Example 4 68.6 Example 5 69.4 Example 6 61.3 Example 7 62.7 Example 8 65.6 Example 9 70.8 Example 10 62.3 Example 11 64.5 Comparative Example 1 39.6 Comparative Example 2 45.1 Comparative Example 3 50.6 Comparative Example 4 49.0 Comparative Example 5 47.6 Comparative Example 6 42.8 Comparative Example 7 43.2 surface

[0126] Test Example 2: Anti-inflammatory test of the composition

[0127] Samples: Softening and brightening compositions prepared in Examples 1-5 and Comparative Examples 1-7;

[0128] Experimental cells: The cell line used was human keratinocyte HaCaT (Shanghai Yaji Biotechnology Co., Ltd.), which was cryopreserved to the 9th generation;

[0129] Reagents and consumables:

[0130] DMSO (dimethyl sulfoxide, analytical grade, used for dissolving samples);

[0131] DMEM culture medium (containing 10% fetal bovine serum, 1% penicillin and antibiotics, v / v, Gibco, catalog number 2105341);

[0132] Capsaicin;

[0133] TNF-α ELISA kit (Shanghai Enzyme Linker, catalog number ml061140);

[0134] 96-well cell culture plates, pipettes, centrifuges, ELISA readers, etc.

[0135] Experimental conditions: Incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%;

[0136] Cells were cultured and treated according to their groups, followed by testing. The testing methods are as follows:

[0137] (1) Sample preparation: The skin care composition samples of Examples 1-11 and Comparative Examples 1-7 were dissolved in dimethyl sulfoxide (DMSO, final concentration ≤0.1%) and adjusted to a final concentration of 1% (v / v) by gradient dilution (DMEM medium containing 10% fetal bovine serum, v / v, 2105341, Gibco). The samples were then sterile filtered through a 0.22 μm filter membrane to obtain the working solution for each group.

[0138] (2) Seed the cell suspension into a 96-well cell culture plate at a density of 2000 cells / well, add 100 μL of culture medium to each well, and culture for 24 h;

[0139] (3) Induction and sample feeding: Discard the supernatant in the well and process according to the groups:

[0140] Control group: 100 μL of DMEM medium (containing 4.6 μg / mL capsaicin) was added to each well.

[0141] Sample group: Add 100 μL of DMEM medium containing 4.6 μg / mL capsaicin and 1 wt% sample working solution to each well.

[0142] The control group and the sample group were incubated in the incubator for another 24 hours.

[0143] (4) Detection of inflammatory factors: Follow the instructions of the ELISA kit (ml061140, Shanghai ELISA reader). Place the ELISA plate in the microplate reader and select 450 nm as the detection wavelength for TNF-α according to the kit instructions. Measure the absorbance (OD value) of each well. Plot the standard concentration as the X-axis and the OD value as the Y-axis, and fit a standard curve using linear regression. Substitute the sample OD values ​​into the standard curve equation to calculate TNF-α (pg / mL). The specific formula is as follows:

[0144] TNF-α inhibition rate (%) = (1 - TNF-α concentration) 对照组 / TNF-α concentration 样品组 )×100%; data are shown in Table 2.

[0145] Table 2. TNF-α inhibition rate data for each group of samples.

[0146] Group / Performance TNF-α Inhibition Rate / % Example 1 67.2 Example 2 63.8 Example 3 65.1 Example 4 57.9 Example 5 59.4 Example 6 47.0 Example 7 47.4 Example 8 55.3 Example 9 62.7 Example 10 50.2 Example 1 152.7 Comparative Example 1 28.5 Comparative Example 2 44.0 Comparative Example 3 36.4 Comparative Example 4 41.2 Comparative Example 5 38.7 Comparative Example 6 31.9 Comparative Example 7 33.2 surface

[0147] As shown in Tables 1-2, and in conjunction with the data from Examples 1 and 2-7, the optimal weight ratio of golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil, and tiger nut oil is (1-2):(0.08-0.15):(0.5-1):(0.2-0.5):(0.1-0.2), and the next optimal ratio is (0.5-5):(0.05-0.5):(0.1-3):(0.1-1):(0.05-0.5). Within the preferred range, the antioxidant and anti-inflammatory effects of the composition are at a relatively good level.

[0148] Based on the data from Examples 1 and 8-9, it can be seen that in step S1 of the preparation of the golden chrysanthemum tea essential oil in Example 8, the amount of spore liquid added was relatively low, the fermentation degree of the golden chrysanthemum tea was relatively low, and the content of theaflavins and flavonoids in the golden chrysanthemum tea essential oil was relatively low. In contrast, the amount of spore liquid added in Example 9 was 30 mL, and its antioxidant properties were similar to those in Example 1. Considering the antioxidant and anti-inflammatory effects and cost, in step S13 of the preparation of golden chrysanthemum tea essence, the spore liquid content per 100g of tea is preferably 25-30 mL.

[0149] Based on the data from Examples 1 and 10, it can be seen that in Example 10, no anhydrous ethanol was added during supercritical CO2 extraction, which affected the antioxidant and anti-inflammatory effects of the composition. This may be because the lack of anhydrous ethanol as an entrainer to enhance the dissolution of polar components resulted in incomplete dissolution of active substances, thus affecting the antioxidant and anti-inflammatory effects.

[0150] Based on the data from Examples 1 and 11, it can be seen that the parameters of dynamic cyclic extraction are also one of the parameters affecting antioxidant performance. The preferred parameters for dynamic cyclic extraction are: pressure of 30-35 MPa, temperature of 40-50℃, CO2 flow rate of 15-18 kg / h, and time of 2.5-3.5h.

[0151] Based on the data from Example 1 and Comparative Examples 1-5, it can be seen that the components in the composition have a significant impact on the antioxidant and anti-inflammatory effects. Golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil have a synergistic effect, and their combined use can improve the antioxidant and anti-inflammatory effects of the composition.

[0152] Based on the data from Example 1 and Comparative Examples 6-7, the antioxidant and anti-inflammatory effects of Comparative Examples 6 and 7 are lower than those of Example 1. This may be because the *Tea laurentii* essential oil in Comparative Example 6 was extracted directly from *Tea laurentii* tea leaves without solid-state fermentation, thus preventing the extraction of large-molecule active ingredients from the tea leaves. In contrast, the *Tea laurentii* tea leaf extract in Comparative Example 7 was extracted using a combination of alcohol and water extraction after solid-state fermentation, resulting in a lower extraction rate of active ingredients. This indicates that the *Tea laurentii* essential oil of this invention first undergoes solid-state fermentation of *Tea laurentii* tea leaves, directionally degrading large-molecule polyphenols into smaller-molecule theaflavins and flavonoids, significantly enhancing its antioxidant capacity and anti-inflammatory activity. Furthermore, the supercritical CO2 extraction process fully preserves volatile terpenes (such as linalool) and thermosensitive active substances under relatively low-temperature conditions, while anhydrous ethanol is used as an entrainer to enhance the dissolution of polar components, ultimately yielding a functional oil with both natural floral fragrance and stability. This component exhibits excellent antioxidant and anti-inflammatory effects.

[0153] Application Example 1-11 and Comparative Application Example 1-7

[0154] The skin-rejuvenating and brightening compositions of Examples 1-11 and Comparative Examples 1-7 were added to the emulsion at a concentration of 4 wt% to obtain the emulsions of Application Examples 1-7 and Comparative Application Examples 1-5. The formulations are shown in Table 3.

[0155] The methods for preparing emulsions in Application Examples 1-11 and Comparative Application Examples 1-7 include the following steps:

[0156] S1. Mix the humectant, thickener, and part of the pH adjuster (EDTA-disodium) with water and stir. Heat to 85°C and homogenize at 13000 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared phase A.

[0157] (2) Mix the emulsifier, heat to 75°C, and homogenize at 13000 rpm for 4 minutes. After homogenization, keep warm for later use to obtain the pre-prepared phase B.

[0158] (3) Mix the preservatives and heat to 60°C to melt them, to obtain the pre-prepared C phase;

[0159] (4) Heat the pre-prepared phase A to 80°C, add the pre-prepared phase B at 300 rpm, stir and mix, then cool down to 60°C, add the pre-prepared phase C at 3000 rpm and stir and mix, then cool down to below 45°C, add the soothing and repairing composition and continue stirring for 8 min, finally add the remaining pH adjuster (arginine) to adjust the pH to 6, then stop stirring, discharge the material, and obtain the emulsion.

[0160] Table 3. Emulsion formulations of Application Examples 1-11 and Comparative Application Examples 1-7

[0161]

[0162] Comparative Application Example 8

[0163] Compared with the skin-brightening composition containing Camellia chrysantha essential oil in Application Example 8, the lotion was prepared by using an equal amount of deionized water instead of the composition, and the preparation method was the same as in Application Example 1.

[0164] Test Example 3: Testing the emulsion's effects on softening, repairing, soothing, and brightening the skin.

[0165] 1. Experimental basis

[0166] The human efficacy evaluation test method shall be followed in accordance with the "Cosmetic Safety Technical Specifications" (2015 edition).

[0167] 2. Subject selection

[0168] Inclusion criteria: We are recruiting Asian adult female volunteers aged 45-60 who meet the following conditions:

[0169] a) Clinical assessment indicates skin laxity (grades I-III according to clinical grading standards);

[0170] b) A self-reported history of skin allergies (confirmed by a dermatologist);

[0171] c) No history of serious systemic diseases or skin diseases.

[0172] Exclusion criteria: pregnant / lactating women, individuals with severe allergies, and individuals who have participated in other clinical trials within the past 3 months.

[0173] Number of participants: A total of 95 qualified volunteers were included and divided into 19 groups of 5 people each using a random number table.

[0174] 3. Sample application method

[0175] Test samples: emulsions prepared in Application Examples 1-11 and Comparative Application Examples 1-8 (double-blind method numbering), wherein the emulsion prepared in Comparative Application Example 8 is the blank control group, and the other application examples and comparative application examples are the sample groups.

[0176] How to use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed.

[0177] 4. Testing Cycle and Process

[0178] Test period: 14 days (D0-D) 14 ).

[0179] Visit time points: D0 (baseline period), D 14 1. Conduct instrument testing.

[0180] Preparation before testing:

[0181] a) After the participants arrived, they used a uniform, non-irritating facial cleanser to clean their faces.

[0182] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0183] c) Keep your eyes closed and relax during the test to avoid facial expressions and movements that may interfere with the process.

[0184] The facial skin condition was assessed 14 days after product use to comprehensively evaluate the product's firming and anti-wrinkle effects. Specific experimental parameters and equipment were used in the experiment.

[0185] Researchers used a Corneometer probe to measure skin moisture content in the cheekbones and a Colorimeter CL440 probe to detect facial luminance (L*) values. Visia-7 was used to photograph volunteers' faces, and image analysis was performed on the exported red area images to obtain a* values. A TewaMeter®™ Hex probe was used to measure transepidermal water loss (TEWL) in the cheekbones. VC20 was used to detect the skin roughness index (SEr) of the cheekbones. Upon the volunteers' first visit, initial values ​​were measured before use, followed by full-face application of the sample. After sitting still for 30 minutes, values ​​were measured again after 30 minutes of sample use.

[0186] Skin moisturizing ability is represented by the improvement in skin moisture content; skin brightening ability is represented by the improvement in L* value; skin soothing ability is represented by the improvement in a* value; skin barrier repair ability is represented by the improvement in TEWL value; and skin softness is represented by the improvement in skin roughness index SEr. The formulas are as follows:

[0187] Skin moisture content improvement rate (%) = | (skin moisture content) 使用后 -Skin moisture content 使用前 Skin moisture content 使用前 ×100%;

[0188] Improvement in facial brightness L* value (%) = | (facial brightness L* value) 使用后 -Face brightness L* value 使用前 )| / facial brightness L* value 使用前 ×100%;

[0189] a* value improvement (%) = | (a* value) 使用后 -a* value 使用前 )| / a*value 使用前 ×100%;

[0190] TEWL value improvement rate (%) = | (TEWL value) 使用后 -TEWL value 使用前 )| / TEWL value 使用前 ×100%;

[0191] Skin roughness index (SEr) improvement rate (%) = |(SEr) 使用后 -SEr 使用前 )| / SEr 使用前 ×100%; the calculation results are shown in Table 4.

[0192] Table 4. Human test results of each emulsion group

[0193] Group / Performance Moisture Improvement Rate / % L* Value Improvement Rate / % a* Value Improvement Rate / % TEWL Improvement Rate / % SEr Improvement Rate / % Application Example 1 42.1 32.4 23.1 30.7 19.7 Application Example 2 40.9 30.5 20.9 29.4 18.0 Application Example 3 41.8 31.1 23.9 31.2 20.2 Application Example 4 36.5 27.2 15.9 25.2 14.3 Application Example 5 37.0 28.3 17.1 26.4 15.6 Application Example 6 31.7 20.8 11.4 20.1 8.3 Application Example 7 32.9 21.9 12.7 21.8 9.5 Application Example 8 35.3 26.7 15.1 24.2 13.5 Application Example 9 39.2 29.3 19.7 28.6 16.1 Application Example 1 0 3 2.0 2 3.4 1 2.0 2 1.8 10.7 Application Example 1 1 3 5.4 2 5.8 1 4.2 2 4.0 1 1.8 Comparison Application Example 1 1 1.4 6.1 4.3 5.7 4.0 Comparison Application Example 2 2 3.8 1 1.9 1 3.6 1 5.1 8.4 Comparison Application Example 3 1 8.5 1 7.3 8.1 1 0.3 1 4.6 Comparison Application Example 4 2 1.9 1 4.9 1 1.2 1 3.5 1 1.4 Comparison Application Example 5 1 9.2 1 3.8 9.4 1 1.6 10.5 Comparison Application Example 6 1 5.0 9.5 5.5 7.7 7.0 Comparison Application Example 7 1 6.8 7.4 7.2 8.0 5.1 Comparison Application Example 8 (Blank Control) 5.6 0.5 1.3 2.1 2.3 surface

[0194] As shown in Figure 2, after 14 days of use, the emulsion of Application Example 1 showed significant improvements in facial brightness, cheek redness, and roughness of the skin on the cheekbone compared to the emulsion of Comparative Application Example 8. This demonstrates that the composition of the present invention achieves soothing, repairing, brightening, and skin-rejuvenating effects.

[0195] As shown in Table 4, Application Example 1 and Comparative Application Examples 1-5 reveal that the skin moisture content, L* value, a* value, TEWL value, and SEr of the emulsion in Application Example 1, which uses a blend of five ingredients—Tea laurel essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil, and tiger nut oil—all significantly increased. This indicates that the five ingredients have a synergistic effect, and the prepared emulsion exhibits excellent soothing, repairing, brightening, and skin-rejuvenating effects.

[0196] Based on the data from Application Examples 1 and 2-7, it can be seen that when the weight ratio of Camellia chrysantha honey, dihydroamaranth alkaloids, Myrothamnus flabellifolia extract and Schisandra chinensis extract is (1-2):(0.08-0.15):(0.5-1):(0.2-0.5):(0.1-0.2), the lotion has better soothing, repairing, brightening and skin-rejuvenating effects.

[0197] Based on the data from Application Example 1 and Comparative Application Examples 6-7, it can be seen that Comparative Application Examples 6-7 lacked the steps of solid-state fermentation of *Tea laurentii* leaves and extraction using supercritical CO2 extraction. The performance of their emulsions was lower than that of Application Example 1. This indicates that the *Tea laurentii* essential oil of the present invention combines microbial fermentation and supercritical CO2 extraction technology in the preparation of *Tea laurentii* essential oil, directionally degrading macromolecular polyphenols into small molecule theaflavins and flavonoid glycosides, significantly improving their antioxidant capacity and anti-inflammatory activity. At the same time, combined with the supercritical CO2 extraction process, volatile terpenes (such as linalool) and thermosensitive active substances are completely preserved under relatively low temperature conditions. Meanwhile, anhydrous ethanol is used as an entrainer to enhance the dissolution of polar components, further improving the performance of the product.

[0198] In summary, the composition of this invention, comprising five components—Tea laurel oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil—forms a synergistic system in an optimal ratio, simultaneously blocking inflammatory roughness and dullness. This overcomes the limitations of traditional products that only target dehydrated roughness, providing a comprehensive, hormone-free solution for sensitive skin. Furthermore, the Tea laurel oil is obtained through a combination of microbial fermentation and supercritical CO2 extraction, resulting in excellent antioxidant and anti-inflammatory effects.

[0199] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil, characterized in that, The product comprises the following components: golden chrysanthemum tea oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil, wherein the weight ratio of the golden chrysanthemum tea oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil is (0.1-10):(0.01-1):(0.01-5):(0.01-5):(0.01-5); wherein the preparation method of the golden chrysanthemum tea oil includes the following steps: S1, solid-state fermentation of golden chrysanthemum tea leaves with *Aspergillus cristatus*; S2, supercritical CO2 extraction of the fermented golden chrysanthemum tea leaves to obtain the golden chrysanthemum tea oil.

2. The skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil as described in claim 1, characterized in that, The weight ratio of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil is (0.5-5):(0.05-0.5):(0.1-3):(0.1-1):(0.05-0.5).

3. The skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil as described in claim 1, characterized in that, The weight ratio of the golden chrysanthemum tea essential oil, ascorbic acid peptide, oat β-glucan, camellia seed oil and tiger nut oil is (1-2):(0.08-0.15):(0.5-1):(0.2-0.5):(0.1-0.2).

4. The skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil as described in claim 1, characterized in that, In step S1 of the preparation of the golden chrysanthemum tea essential oil, the specific steps include: S11, inoculating *Aspergillus cristatus* onto a PDA solid culture medium for cultivation; after cultivation, washing the culture with sterile water; then dispersing the spores evenly by vortexing and shaking to obtain a spore solution; and adjusting the spore solution concentration to 10% with sterile water. 6 -10 8 S12: Wash the fresh leaves of Camellia chrysantha and dry them until the moisture content is 10-20%; S13: Mix the spore liquid and the dried Camellia chrysantha leaves evenly, add 10-30 mL of spore liquid to every 100 g of tea leaves, and carry out solid-state fermentation under sealed conditions to obtain the fermented Camellia chrysantha leaves; wherein, the solid-state fermentation temperature is 26-32℃, the humidity is 80-85%, and the time is 8-20 days.

5. The skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil as described in claim 1, characterized in that, In step S2 of the preparation of the golden chrysanthemum tea essential oil, the specific steps include: S21: Raw material pretreatment: Fermented golden chrysanthemum tea leaves are freeze-dried under vacuum to reduce moisture content to ≤8%, then extruded and puffed using a low-temperature screw extruder. After puffing, the leaves are pulverized to 30-40 mesh to obtain golden chrysanthemum tea powder. The golden chrysanthemum tea powder is then uniformly filled into the extraction vessel. The vacuum freeze-drying temperature is -50℃ to -30℃, the time is 18-28 hours, and the vacuum degree is 80 ± 20°C. Pa; The temperature of low-temperature screw extrusion puffing is 40-50℃, the pressure is 0-1MPa, and the time is 5-10min; S22: Supercritical extraction: Using food-grade CO2 as solvent and anhydrous ethanol as entrainer, Camellia chrysantha powder is subjected to dynamic circulation extraction in intermittent mode to obtain CO2 fluid containing essential oil components; wherein, the purity of the food-grade CO2 is ≥99.9%, and the volume of anhydrous ethanol accounts for 4-6% of the total volume of food-grade CO2; the intermittent mode is extraction for 35-45min followed by standing for 5-15min; the pressure of dynamic circulation extraction is 20-35 MPa, the temperature is 20-50℃, the CO2 flow rate is 10-20 kg / h, and the time is 1-5h; S23: Separation and purification: The CO2 fluid containing essential oil components is introduced into a two-stage separation system: the first stage separation removes wax and resin, the second stage separation collects the essential oil main body containing terpenes and flavonoids, then adsorbs residual anhydrous ethanol, and the ethanol residue is reduced to <100% by nitrogen purging. The essential oil of the golden chrysanthemum tea was obtained by measuring ppm; wherein the pressure of the first separation was 6-7 MPa and the temperature was 35-40℃; the pressure of the second separation was 0.05-0.2 MPa and the temperature was 4-6℃.

6. The use of the skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil according to any one of claims 1-5 in the preparation of cosmetics.

7. The application of the skin-rejuvenating and brightening composition containing golden chrysanthemum tea essential oil as described in claim 6 in the preparation of cosmetics, characterized in that, The cosmetic is a toner, lotion, cream, mask, serum, or spray, and the amount of the composition added is 1%-5% of the total weight of the cosmetic.

8. An emulsion, characterized in that, The ingredients include the following ingredients by weight percentage: 1%-5% of the skin-brightening composition containing golden chrysanthemum tea essential oil as described in any one of claims 1-5, 0.5%-1% thickener, 3%-5% emulsifier, 0.5%-5% moisturizer, 0.5%-3% preservative, and 0.01%-0.3% pH adjuster, with the balance being deionized water.

9. The emulsion as described in claim 8, characterized in that, The raw materials are selected from at least one of (I)-(V): (I) The humectant is at least one of glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, and allantoin; (II) The thickener is carbomer and / or xanthan gum; (III) The emulsifier is at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, isononyl isononate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethyl silsesquioxane, pentaerythritol distearate, and sucrose stearate; (IV) The pH adjuster includes at least one of arginine, tromethamine, and disodium EDTA; (V) The preservative includes at least one of 1,2-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

10. The method for preparing the emulsion according to claim 8, characterized in that, The process includes the following steps: (1) Mixing and stirring the humectant, thickener, and part of the pH adjuster with water, heating to 80-90°C, homogenizing, and keeping warm after homogenization to obtain a pre-prepared phase A; (2) Mixing the emulsifier, heating to 70-80°C, homogenizing, and keeping warm after homogenization to obtain a pre-prepared phase B; (3) Mixing the preservative, heating to 55-65°C to melt, and obtaining a pre-prepared phase C; (4) Heating the pre-prepared phase A to 75-85°C, adding the pre-prepared phase B, stirring and mixing, then cooling to 55-65°C, adding the pre-prepared phase C, stirring and mixing, then cooling to below 45°C, adding the skin-brightening composition containing golden tea essential oil, continuing to stir, and finally adding the remaining pH adjuster to adjust the pH to 5.5-7.0, then stopping the stirring, discharging the material, and obtaining the emulsion.

Images