Water-oil balance composition for sensitive skin and use thereof

Abstract

The application relates to the cosmetic technical field, and particularly discloses a water-oil balance composition suitable for sensitive skin and application thereof. The components in a suitable mass ratio range are matched with each other, so that the temperature-sensitive receptors of the skin can be synergistically regulated, natural moisturizing production is promoted, and ultraviolet protection is improved. The water-oil balance composition can selectively inhibit TRPV1-mediated 5alpha-reductase activity and ultraviolet-induced oxidative stress under high temperature in summer, accurately regulate sebum secretion without damaging the barrier, and promote the generation of natural moisturizing factors by activating filaggrin degradation in a low-temperature environment in winter, dynamically adjust the hydration degree of the stratum corneum, break through the technical limitations of traditional products, such as seasonal use and single and one-sided functions of active ingredients, realize cross-seasonal balanced care of a single formula, and are mild and non-irritating, and are suitable for sensitive skin.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and in particular to water-oil balancing compositions suitable for sensitive skin and their applications. Background Technology

[0002] The root cause of combination skin's "oily in summer, dry in winter" pattern lies in the imbalance between sebaceous gland function and the skin barrier's homeostasis in response to environmental changes. High summer temperatures activate the epidermal TRPV1 channel and UV-induced 5α-reductase activity, leading to excessive sebum production. Conversely, low winter temperatures inhibit ceramide synthase and filaggrin degrading enzymes, reducing natural moisturizing factors (NMF) and causing dryness and flaking. Combination sensitive skin, due to its sensitivity and fragility, overreacts to changes in temperature and humidity. In summer, it is more prone to excessive sebum secretion, even leading to seborrheic dermatitis, while in winter it is easily accompanied by dryness and flaking, creating a vicious cycle of "oil-dry-sensitivity."

[0003] Currently, summer oil-control products rely on astringent ingredients such as salicylic acid and aluminum chloride to control oil by inhibiting sebaceous gland cell activity or mechanically clogging pores. However, these ingredients can easily exacerbate barrier damage in winter and stimulate TRPV1, causing stinging in sensitive skin. Winter moisturizing products mainly consist of occlusive oils and humectants, which can temporarily improve the hydration of the stratum corneum, but in the high temperatures of summer, they hinder sweat evaporation, inducing the risk of "acne breakouts." In addition, current skincare products lack the ability to dynamically respond to temperature / UV signals and cannot intelligently switch between oil-control and moisturizing modes according to environmental changes. Summary of the Invention

[0004] The purpose of this application is to overcome the shortcomings of the prior art and provide a water-oil balancing composition suitable for sensitive skin and its application. The water-oil balancing composition of this application can simultaneously maintain the seasonal balance of sebum secretion and skin moisture content, thereby achieving intelligent regulation of "oil control without dryness in summer and moisturizing without acne in winter," while meeting the gentle needs of sensitive skin.

[0005] To achieve the above objectives, the technical solution adopted in this application is as follows:

[0006] This application provides a water-oil balancing composition suitable for sensitive skin, the water-oil balancing composition comprising active ingredients derived from medicinal Fomitopsis, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract;

[0007] The mass ratio of the active ingredient of the medicinal Fomitopsis pilosula, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (0.5~10):(0.1~5):(0.01~2):(0.01~5):(0.01~2).

[0008] The active ingredients derived from medicinal fusiforme include at least one of medicinal fusiforme extract and medicinal fusiforme-Lactobacillus plantarum bidirectional fermentation product.

[0009] In the technical solution of this application, the components within the above-mentioned mass ratio range are combined to synergistically regulate the skin's temperature-sensitive receptors, promote the generation of natural moisturizing factors, and enhance UV protection. The water-oil balancing composition provided by this application can selectively inhibit TRPV1-mediated 5α-reductase activity and UV-induced oxidative stress in the high temperatures of summer, precisely regulate sebum secretion without damaging the skin barrier, and at the same time, promote the generation of natural moisturizing factors by activating filaggrin degradation in the low-temperature environment of winter, dynamically regulate the hydration of the stratum corneum, and overcome the technical limitations of traditional products that are used seasonally and have single-function active ingredients. It achieves cross-seasonal balanced care with "single-formula self-adaptation" and is gentle and non-irritating, suitable for sensitive skin.

[0010] In the technical solution of this application, the active ingredients derived from medicinal Fomitopsis are rich in triterpenoids and polysaccharides. They balance sebum secretion by inhibiting 5α-reductase activity and regulating the miR-29 signaling pathway. At the same time, the polysaccharides can form a water-retaining film to prevent moisture loss. 4-tert-butylcyclohexanol, as an antagonist of the temperature-sensitive receptor TRPV1, can quickly relieve excessive oil production and skin sensitivity caused by high summer temperatures. The selected hyaluronic acid has a low molecular weight and can penetrate into the granular layer to activate the expression of filaggrin degrading enzyme Caspase-14, promoting the seasonal adaptive synthesis of natural moisturizing factor (NMF). Milk thistle seed extract is rich in silymarin and minerals, which not only provide strong antioxidant protection for the skin but also effectively resist the damage of summer ultraviolet rays and inhibit elastase activity to protect the skin cell extracellular matrix. Sophora flavescens root extract contains matrine and flavonoids, which have anti-inflammatory and antibacterial effects and can help regulate sebum secretion and reduce acne. While the components possess the aforementioned effects, there are still unpredictable synergistic or antagonistic effects between the components. However, based on the component combination and mass ratio provided in this application, the water-oil balance composition can ultimately maintain the seasonal balance of sebum secretion and skin moisture content, thereby achieving intelligent regulation of "oil control without drying in summer and moisturizing without acne in winter," while also meeting the gentle needs of sensitive skin.

[0011] As a preferred embodiment of the water-oil balancing composition suitable for sensitive skin described in this application, the mass ratio of the medicinal Fomitopsis pilosula active ingredient, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (1~8):(0.2~2):(0.05~0.5):(0.1~1):(0.05~0.5).

[0012] This study found that when the mass ratio of the components in the water-oil balancing composition is further selected within the above-mentioned range, the synergistic effect of the components in the water-oil balancing composition is better. The resulting water-oil balancing composition not only has better oil control in summer and moisturizing effect in winter, but also better UV protection effect. The water-oil balancing composition provided by this application can selectively inhibit TRPV1-mediated 5α-reductase activity and UV-induced oxidative stress under high summer temperatures, precisely regulate sebum secretion without damaging the skin barrier. At the same time, in low winter environments, it can promote the generation of natural moisturizing factors by activating filaggrin degradation and dynamically regulate the hydration of the stratum corneum, achieving cross-seasonal balanced care, and is suitable for sensitive skin.

[0013] As a preferred embodiment of the water-oil balancing composition suitable for sensitive skin described in this application, the mass ratio of the medicinal Fomitopsis pilosula active ingredient, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (3~5):(0.5~1):(0.2~0.3):(0.3~0.5):(0.1~0.2).

[0014] When the water-oil balancing composition of this application is preferably within the above-mentioned mass ratio range, it can better achieve intelligent regulation of "controlling oil without drying out in summer and moisturizing without causing acne in winter", and can also improve the product's UV protection effect, meeting the gentleness needs of sensitive skin.

[0015] Among them, when the active ingredients of medicinal Fomitopsis thunbergii and Sophora flavescens root extract are combined, the resulting water-oil balance composition can better regulate oil control and moisturizing effects across seasons, while also providing better UV protection.

[0016] In some specific embodiments, the active ingredient derived from the medicinal blastocyst is a bidirectional fermentation product of medicinal blastocyst and Lactobacillus plantarum.

[0017] This application utilizes active ingredients derived from medicinal stratafoetida, which can be well combined with other components. In the high temperatures of summer, it precisely regulates sebum secretion without damaging the skin barrier and enhances UV protection. At the same time, in the low-temperature environment of winter, it dynamically regulates the hydration of the stratum corneum, achieving balanced care across seasons. Furthermore, the preferred active ingredient derived from medicinal stratafoetida is a bidirectional fermentation product of medicinal stratafoetida and Lactobacillus plantarum, which further enhances the above-mentioned effects.

[0018] The method for preparing the medicinal Fertilizer of Polyporus flocculation and Lactobacillus plantarum bidirectional fermentation product includes the following steps:

[0019] (1) Mix Lactobacillus plantarum culture and medicinal fibroblasts at a mass ratio of (0.5~3):1 and then anaerobic ferment. The anaerobic fermentation temperature is 37±2℃ and the anaerobic fermentation time is 48~72h to obtain the fermentation product.

[0020] (2) After cooling the fermentation product to 2~6℃, it is circulated in a microfluidic homogenizer. The pressure of circulation is 1200~1400 bar and the temperature of circulation is 20~30℃. Finally, it is crushed and centrifuged, the supernatant is collected and dried to obtain the medicinal Fertilizer-Lactobacillus plantarum bidirectional fermentation product.

[0021] In some specific embodiments, the preparation method of medicinal Fomitopsis thaliana extract includes the following steps:

[0022] The dried fruiting bodies of medicinal Fomitopsis are pulverized and passed through an 80-mesh sieve. They are then added to a 70% ethanol aqueous solution for ultrasonic extraction. The material-to-liquid ratio is 1g:30mL. The ultrasonic extraction temperature is 40℃, the power is 120W, and the time is 30min. After extraction, the mixture is centrifuged, the supernatant is collected, concentrated, and dried to obtain the medicinal Fomitopsis extract.

[0023] As a preferred embodiment of the water-oil balancing composition suitable for sensitive skin described in this application, the preparation method of the Sophora flavescens root extract includes the following steps:

[0024] After drying and pulverizing the root of Sophora flavescens, it was added to a eutectic solvent for ultrasonic extraction. After extraction, the root was centrifuged, the supernatant was collected, concentrated and dried to obtain Sophora flavescens root extract.

[0025] The eutectic solvent includes citric acid and sorbitol.

[0026] This application research found that the preparation method of Sophora flavescens root extract, which uses optimized conditions such as eutectic melting and ultrasonic extraction, can improve extraction efficiency and also extract active components that synergistically interact with other components, thereby improving the overall effect of the water-oil balance composition.

[0027] Using the optimized preparation method of Sophora flavescens root extract, the obtained Sophora flavescens root extract, when used in combination with active ingredients derived from medicinal Fomitopsis thaliana, can selectively inhibit TRPV1-mediated 5α-reductase activity and UV-induced oxidative stress. This precisely regulates sebum secretion and enhances UV protection under high summer temperatures. Simultaneously, by activating filaggrin degradation and promoting the generation of natural moisturizing factors, it dynamically regulates the hydration of the stratum corneum in winter environments. This better overcomes the technical limitations of traditional products that are used seasonally and have single-function active ingredients, achieving "single-formula self-adaptive" cross-seasonal balanced care that is gentle and non-irritating, suitable for sensitive skin.

[0028] As a preferred embodiment of the water-oil balancing composition suitable for sensitive skin described in this application, the method for preparing the eutectic solvent includes the following steps: mixing citric acid and sorbitol in a molar ratio of 1:(1~2), and then adding ultrapure water to obtain the eutectic solvent; in the eutectic solvent, the mass percentage of water is 15~40%.

[0029] This application uses the above-mentioned types and specific molar ratios of eutectic solvents, which can improve the extraction efficiency of Sophora flavescens root extract and enhance the oil control, moisturizing and UV protection effects of Sophora flavescens root extract in water-oil balance compositions.

[0030] In some specific embodiments, the molar ratio of citric acid to sorbitol is 1:1; and the mass percentage of water in the eutectic solvent is 25%.

[0031] As a preferred embodiment of the water-oil balancing composition suitable for sensitive skin described in this application, the mass-volume ratio of the Sophora flavescens root and the eutectic solvent is 1g:(20~50)mL, preferably 1g:40mL.

[0032] The Sophora flavescens root and the eutectic solvent in this application are within the above-mentioned mass-volume ratio range, and the resulting Sophora flavescens root extract has better performance when applied to water-oil balance compositions.

[0033] As a preferred embodiment of the water-oil balancing composition suitable for sensitive skin described in this application, the ultrasonic extraction temperature is 30~50℃, the power is 100~150W, and the time is 30~90 min; preferably, the ultrasonic extraction temperature is 40℃, the power is 120 W, and the time is 40 min.

[0034] The ultrasonic extraction conditions described above can improve the extraction efficiency of Sophora flavescens root extract, thereby improving the overall performance of the water-oil balance composition.

[0035] This application also provides the use of a water-oil balancing composition suitable for sensitive skin in the preparation of skin care products.

[0036] The water-oil balancing composition provided in this application can be applied to oil-controlling, moisturizing, and repairing products, and further applied to people with sensitive skin.

[0037] As a preferred embodiment of the application described in this application, the skin care product includes at least one of toner, lotion, cream, mask, serum, and spray.

[0038] The water-oil balancing composition provided in this application can selectively inhibit TRPV1-mediated 5α-reductase activity and UV-induced oxidative stress, and can also promote the generation of natural moisturizing factors by activating filaggrin degradation, thereby achieving precise cross-seasonal regulation of sebum secretion and adjustment of stratum corneum hydration, and enhancing UV protection. Therefore, the water-oil balancing composition provided in this application can be widely used in the preparation of skin care products.

[0039] This application also provides a face cream comprising the following components by weight percentage: 1-10% water-oil balancing composition, 0.1-1% thickener, 0.5-5% moisturizer, 0.5-5% emulsifier, 0.01-0.3% pH adjuster, 0.5-3% preservative, and the balance being deionized water.

[0040] In some specific embodiments, the thickener includes at least one of polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, saffron gum, guar gum, ammonium acryloyl dimethyl taurate / VP copolymer, and acrylate copolymers.

[0041] In some specific embodiments, the moisturizer includes at least one of glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, sodium polyacrylate, and hydrogenated lecithin.

[0042] In some specific embodiments, the emulsifier includes at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0043] In some specific embodiments, the pH adjuster includes at least one of arginine, disodium ethylenediaminetetraacetate, tromethamine, and disodium EDTA.

[0044] In some specific embodiments, the preservative includes at least one of p-hydroxyacetophenone and polyols.

[0045] Compared with the prior art, this application has the following beneficial effects:

[0046] This application provides a water-oil balancing composition suitable for sensitive skin and its application. By combining components in a suitable mass ratio range, this application can synergistically regulate the skin's temperature-sensitive receptors, promote natural moisturizing production, and enhance UV protection. The water-oil balancing composition provided by this application can selectively inhibit TRPV1-mediated 5α-reductase activity and UV-induced oxidative stress in the high temperatures of summer, precisely regulating sebum secretion without damaging the skin barrier. At the same time, in the low-temperature environment of winter, it promotes the production of natural moisturizing factors by activating filaggrin degradation and dynamically regulates the hydration of the stratum corneum. This overcomes the technical limitations of traditional products that are used seasonally and have single-function active ingredients, achieving "single-formula adaptive" cross-seasonal balanced care. It is also gentle and non-irritating, suitable for sensitive skin. Detailed Implementation

[0047] To better illustrate the purpose, technical solution, and advantages of this application, the following will provide further explanation of this application in conjunction with specific embodiments.

[0048] In the following examples and comparative examples, unless otherwise specified, the experimental methods used are conventional methods, and the materials and reagents used are commercially available unless otherwise specified. Furthermore, the raw materials used in each parallel experiment are the same.

[0049] 4-tert-butylcyclohexanol: purchased from Guangdong Qianjin Chemical Reagent Co., Ltd., product number B2025012011;

[0050] Hyaluronic acid: purchased from Xi'an Yahua Biotechnology Co., Ltd., product number YH22071304;

[0051] Milk thistle seed extract: purchased from Xi'an Shizeyuan Biotechnology Co., Ltd., product number SFJS01;

[0052] Medicinal Fomitopsis extract: prepared in-house, the preparation method includes the following steps:

[0053] The dried fruiting bodies of medicinal Fomitopsis (purchased from Anhui Boman Pharmaceutical Co., Ltd.) were pulverized and passed through an 80-mesh sieve. They were then added to a 70% ethanol aqueous solution for ultrasonic extraction. The material-to-liquid ratio was 1g:30mL. The ultrasonic extraction temperature was 40℃, the power was 120W, and the time was 30min. After extraction, the mixture was centrifuged, the supernatant was collected, concentrated, and dried to obtain the medicinal Fomitopsis extract.

[0054] Medicinal Fermentation Product of *Polyporus flocculationii* and *Lactobacillus plantarum*: Prepared in-house, the preparation method includes the following steps:

[0055] (1) Inoculate Lactobacillus plantarum (ATCC BAA-793) into MRS liquid medium (purchased from Thermo Fisher Scientific) at an inoculation rate of 3% (V / V) and anaerobic ferment at 37°C for 20 h until the OD600 value is 1.4 to obtain Lactobacillus plantarum bacterial culture.

[0056] (2) The dried fruiting bodies of medicinal fumonis (purchased from Anhui Boman Pharmaceutical Co., Ltd.) were pulverized to 40 mesh and then sterilized at 121°C for 15 min to obtain medicinal fumonis powder.

[0057] (3) Mix medicinal fumonis powder and Lactobacillus plantarum liquid at a mass ratio of 1:2, and add 2% g / mL glucose and 0.05% g / mL magnesium sulfate based on the total volume of medicinal fumonis powder and Lactobacillus plantarum liquid. Then, anaerobic ferment at 37℃ for 60 h to obtain fermentation product.

[0058] (4) After the fermentation product was pre-cooled to 4°C, it was circulated 4 times using a microfluidic homogenizer at 1200 bar pressure, 600 rpm stirring speed and 25°C temperature. After the circulation treatment, it was crushed and then centrifuged at 12000 g speed for 20 min. The supernatant was collected and vacuum dried at 25°C to obtain active ingredient 1 from medicinal Fertilizer.

[0059] Sophora flavescens root extract 1: self-prepared, the preparation method includes the following steps:

[0060] 1) Citric acid and sorbitol are mixed in a 1:1 molar ratio, and then ultrapure water is added to obtain a eutectic solvent; the water content in the eutectic solvent is 25% by mass.

[0061] 2) After drying and pulverizing the Sophora flavescens root, add it to a eutectic solvent for ultrasonic extraction. The mass-volume ratio of the Sophora flavescens root to the eutectic solvent is 1g:40mL. The ultrasonic extraction temperature is 40℃, the power is 120W, and the time is 40min. After extraction, centrifuge, collect the supernatant, concentrate and dry it to obtain Sophora flavescens root extract.

[0062] Sophora flavescens root extract 2: self-made. The difference between the preparation method and that of Sophora flavescens root extract 1 is that in step 1), citric acid and sorbitol are mixed in a molar ratio of 1:4.

[0063] Sophora flavescens root extract 3: self-made. The preparation method is different from that of Sophora flavescens root extract 1 in that, in step 1), betaine and glycerol glucoside are mixed in a 1:1 molar ratio and then ultrapure water is added to obtain a eutectic solvent.

[0064] Sophora flavescens root extract 4: self-made. The difference between the preparation method and that of Sophora flavescens root extract 1 is that the mass-volume ratio of Sophora flavescens root and eutectic solvent in step 2) is 1g:10mL.

[0065] Sophora flavescens root extract 5: self-made. The difference between the preparation method and that of Sophora flavescens root extract 1 is that the ultrasonic extraction temperature in step 2) is 60℃, the power is 160W, and the time is 40 min.

[0066] Sophora flavescens root extract 6: self-made. The difference between the preparation method and that of Sophora flavescens root extract 1 is that a 75% ethanol aqueous solution is used instead of a eutectic solvent.

[0067] Examples 1-8 and Comparative Examples 1-7

[0068] This application provides an embodiment and comparative example of a water-oil balancing composition suitable for sensitive skin, the components (parts by mass) of which are shown in Table 1.

[0069] Table 1

[0070]

[0071] The method for preparing the water-oil balance composition provided in Example 1 is as follows: the components are mixed to obtain the water-oil balance composition.

[0072] The preparation methods of the water-oil balance compositions provided in Examples 2-8 and Comparative Examples 1-9 are consistent with those in Example 1; if the relevant components are not available, they can be omitted.

[0073] Example 9

[0074] This application provides a water-oil balance composition, the only difference between the water-oil balance composition and Example 1 is that Sophora flavescens root extract 2 is used instead of Sophora flavescens root extract 1.

[0075] Example 10

[0076] This application provides a water-oil balance composition, the only difference between the water-oil balance composition and Example 1 is that Sophora flavescens root extract 3 is used instead of Sophora flavescens root extract 1.

[0077] Example 11

[0078] This application provides a water-oil balance composition, the only difference between the water-oil balance composition and Example 1 is that Sophora flavescens root extract 4 is used instead of Sophora flavescens root extract 1.

[0079] Example 12

[0080] This application provides a water-oil balance composition, the only difference between the water-oil balance composition and Example 1 is that Sophora flavescens root extract 5 is used instead of Sophora flavescens root extract 1.

[0081] Comparative Example 10

[0082] This application provides a water-oil balance composition in comparison, the only difference between the water-oil balance composition and Example 1 is that Sophora flavescens root extract 6 is used instead of Sophora flavescens root extract 1.

[0083] Comparative Example 11

[0084] This application provides a comparative example of a water-oil balance composition, the only difference between the water-oil balance composition and Example 1 being the use of Lactobacillus / North American Sacred Grass Ferment Extract (purchased from Synsil, Inc., USA) instead of the active ingredient derived from medicinal Ferula meliflora.

[0085] Comparative Example 12

[0086] This application provides a water-oil balance composition in comparison, the only difference between the water-oil balance composition and Example 1 being the use of tetrandrine (purchased from Shaanxi Shize Tiancheng Biotechnology Co., Ltd.) instead of 4-tert-butylcyclohexanol.

[0087] Comparative Example 13

[0088] This application provides a comparative example of a water-oil balance composition, the only difference between the water-oil balance composition and Example 1 being the use of methyl propylene glycol (purchased from Shandong Taixi Chemical Co., Ltd.) instead of hyaluronic acid.

[0089] Comparative Example 14

[0090] This application provides a water-oil balance composition in comparison, the only difference between the water-oil balance composition and Example 1 is that perilla seed extract (purchased from Shaanxi Jianchuang Biotechnology Co., Ltd.) is used instead of milk thistle seed extract.

[0091] Application Examples 1-14, Comparison of Application Examples 1-14 and Blank Application Example

[0092] This application provides a face cream in the application examples, comparative application examples, and blank application examples. The components (mass percentage) of the face cream are shown in Table 2. In application examples 1 to 12, the water-oil balancing compositions used are the water-oil balancing compositions prepared in examples 1 to 12, for example, application example 1 uses the water-oil balancing composition in example 1, application example 2 uses the water-oil balancing composition in example 2, and so on. In comparative application examples 1 to 14, the water-oil balancing compositions used are the water-oil balancing compositions prepared in comparative examples 1 to 14. In application examples 13 to 14, the water-oil balancing compositions used are the water-oil balancing compositions prepared in example 1.

[0093] Table 2

[0094]

[0095] The method for preparing the face cream provided in Example 1 includes the following steps:

[0096] (1) Mix the humectant and thickener with water and stir. Heat to 85±2℃ and homogenize at 1200 rpm for 4 min. After homogenization, keep warm for later use to obtain pre-prepared component A.

[0097] (2) Mix the emulsifier, heat to 85±2℃, and homogenize at 1200 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared component B.

[0098] (3) Mix the preservatives and heat to 60±2℃ to melt them, to obtain the pre-prepared component C;

[0099] (4) Heat the pre-prepared component A to 80±2℃, add the pre-prepared component B at 250 rpm and stir to mix. Then cool down to 60±2℃ and add the pre-prepared component C at 250 rpm and stir to mix. Then cool down to 40℃ and add the water-oil balance composition and continue stirring for 8 min. Finally, add the remaining pH adjuster to adjust the pH to 6.0, then stop stirring, discharge the material, and obtain the face cream.

[0100] The preparation methods of the face creams provided in Application Examples 2-14, Comparative Application Examples 1-14, and Blank Application Examples are consistent with those in Application Example 1. If the relevant components are not available, they can be omitted.

[0101] Example of effect 1

[0102] This application investigates the effects of the water-oil balance compositions prepared in Examples 1-12 and Comparative Examples 1-14, including the following aspects:

[0103] 1. In vitro experiments have shown that the water-oil balance composition inhibits 5α-reductase, and the inhibitory effect is higher at high temperatures than at low temperatures:

[0104] Seasonal differences in skin oil production are closely related to the activity of temperature-regulated 5α-reductase. This enzyme converts testosterone to dihydrotestosterone, directly stimulating sebaceous gland proliferation and lipid synthesis. Previous studies have shown that high summer temperatures significantly increase 5α-reductase activity, leading to excessive skin oil production. This experiment simulated summer (38℃) and winter (16℃) temperatures to determine the inhibitory effect of the composition on 5α-reductase under different conditions. The composition's inhibitory effect on 5α-reductase was higher in high summer temperatures than in low winter temperatures, indicating that the composition possesses "temperature-sensitive oil-controlling" properties. The test method is as follows:

[0105] (1) Preparation of experimental reagents: Weigh 1.00g of the water-oil balance composition prepared in the examples and comparative examples, dissolve it in phosphate buffer and make up to 100 mL to obtain a sample working solution with a concentration of 1.0%; weigh 0.42g of NADPH disodium salt powder, dissolve it in phosphate buffer and make up to 100 mL to obtain a NADPH solution with a concentration of 5mM; accurately weigh 5.8mg of testosterone powder, dissolve it in 1mL of DMSO, add phosphate buffer and make up to 100mL to obtain a testosterone solution with a concentration of 0.2mM;

[0106] (2) Reaction system: For the sample group, add 1 mL of sample working solution, 1 mL of enzyme solution (5α-reductase concentration of 1 mg / mL, sourced from Guangdong Pharmaceutical University), 1 mL of NADPH solution and 1 mL of testosterone solution to a test tube; for the blank group, add 1 mL of phosphate buffer, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution to a test tube.

[0107] (3) Detection of 5α-reductase inhibition rate: Gently shake the solution in the test tube, pipette 200 μL into a 96-well microplate, place it in a microplate reader, and measure the absorbance at 340 nm. This is the first measurement value A. 样品0 (Sample group) and A 酶0 (Blank group); After incubation at 38℃ (simulating summer high temperature) or 16℃ (simulating winter low temperature) for 20 minutes, the samples were then analyzed using a microplate reader. The absorbance was measured at 340nm, and this value was recorded as the second measurement, A. 样品20 (Sample group) and A 酶20 (Blank group); Each sample was tested in triplicate, and the average value was taken. The calculation formula is as follows:

[0108]

[0109] The results are shown in Table 3.

[0110] Table 3

[0111]

[0112] The results, as shown in Table 3, indicate that the water-oil balance compositions obtained in Examples 1-12 of this application exhibit higher inhibitory capacity against 5α-reductase at high temperatures in summer than at low temperatures in winter, demonstrating that the water-oil balance compositions of this application possess "temperature-sensitive oil control" characteristics. Specifically, the water-oil balance compositions of this application achieve an inhibition rate of over 38.4% against 5α-reductase under high-temperature conditions and an inhibition rate of less than 10.8% under low-temperature conditions. The water-oil balance compositions obtained in Examples 1-7 show inhibition rates of 38.4%-84.3% against 5α-reductase at high temperatures and 5.1%-10.8% against 5α-reductase at low temperatures, with a high-temperature inhibition rate / low-temperature inhibition rate of 44-16.5. Among these, the water-oil balance composition of Example 1 demonstrates the best "temperature-sensitive oil control" effect.

[0113] The water-oil balance composition prepared in Example 1 showed better inhibitory activity against 5α-reductase than that in Examples 9-12, indicating that the preparation method of Sophora flavescens root extract affects the oil control performance of the composition.

[0114] Furthermore, as can be seen from Comparative Examples 1 and 1-7, the effects of this application cannot be achieved when one of the components is not added, or when other components are used to make up the difference while the component is not added. As can be seen from Examples 1 and 8-9, the effects of this application cannot be achieved when the mass fraction of the components in Comparative Examples 8-9 is not within the range given in this application. As can be seen from Examples 1 and 10-14, the effects of this application cannot be achieved when other similar components are used as substitutes.

[0115] 2. Cell experiments have demonstrated that the composition has a "seasonal oil-controlling" effect by regulating the temperature-sensitive receptor TRPV1 and ultraviolet protection.

[0116] Seasonal sebum secretion imbalance in the skin is closely related to the temperature-sensitive receptor TRPV1 and UV-induced oxidative stress. High temperatures and UV radiation in summer synergistically activate the TRPV1 channel, triggering calcium ion influx and upregulating fatty acid synthase (FASN) expression, leading to excessive sebum secretion. Low temperatures in winter reduce sebum synthesis by inhibiting PPARγ signaling. This experiment simulated summer (38℃ + UVB) and winter (33℃ + darkness) environments to verify the bidirectional regulatory effect of the composition on TRPV1 pathway inhibition, UV damage repair, and seasonal sebum secretion in SZ95 sebaceous gland cells. The lower sebum secretion under simulated summer conditions compared to winter indicates that the composition has a "seasonal oil-controlling" effect.

[0117] The cell line used was human sebaceous gland cell line SZ95 (Shanghai Qingqi Biotechnology, BFN60807569), passaged 7 times. Summer testing conditions were: incubator temperature 38℃, CO2 5±1%, UVB irradiation (20mJ / cm², wavelength 310nm); winter testing conditions were: incubator temperature 33℃, CO2 5±1%, dark environment. Cells were cultured according to groups, followed by testing, specifically TRPV1 expression detection, reactive oxygen species (ROS) level detection, and neutral lipid content detection. The testing methods are as follows:

[0118] (1) Seed the cell suspension into a 96-well cell culture plate at a density of 1.0 x 10⁶ cells per well. 4 Each well contains 100 μL of DMEM medium (Gibco, C11965500BT) and is incubated for 24 h under summer or winter test conditions.

[0119] (2) Discard the supernatant, add 100 μL of culture medium to the control group, and add 100 μL of culture medium containing 0.2% w / v (g / mL) of the water-oil balance composition prepared in the examples and comparative examples to the sample groups respectively;

[0120] (3) After incubating under the same conditions for another 48 hours, collect the cells, discard the supernatant, and use the cells for subsequent detection;

[0121] (4) TRPV1 expression detection: Cells were lysed with RIPA lysis buffer containing protease inhibitor (Shanghai Merck, V900854). After lysis, the samples were centrifuged at 12000g for 5 minutes, and the supernatant was collected. The TRPV1 protein level in the supernatant of each group of cells was detected using the human conversion receptor potential cation channel subfamily V member 1 (TRPV1) ELISA kit (Shanghai Enzyme-Linked Biotechnology, ml106587). The results were calculated using absorbance OD value. The reduction rate of intracellular TRPV1 protein (%) = (1 - OD value of sample group / OD value of control group) * 100%.

[0122] (5) Detection of reactive oxygen species (ROS) level: The reactive oxygen species (ROS) test kit (Shanghai Enzyme-Linked Biotechnology, ml092661) was used to detect the oxidative stress level of cells in each group. The specific operation was as follows: the cell pellet was resuspended with DCFH-DA probe solution, incubated at 37℃ for 1 h, centrifuged at 1000g for 5 minutes, the supernatant was removed and the cell pellet was collected. The pellet was washed twice with PBS and the fluorescence was detected using a multi-functional microplate reader. The excitation wavelength was 500 nm and the absorption wavelength was 525 nm. The results were calculated using the fluorescence OD value. The reduction rate of intracellular reactive oxygen species (ROS) level (%) = (1 - OD value of sample group / OD value of control group) * 100%.

[0123] (6) Detection of neutral lipid content: Nile red dye (Shanghai Yuanye Biotechnology, S02N11G129713) was added to the cells and incubated at 37℃ in the dark for 15 min. The cells were washed twice with PBS, and the fluorescence was detected using a multi-functional microplate reader. The excitation wavelength was 485 nm and the absorption wavelength was 565 nm. The results were calculated using the fluorescence OD value. The reduction rate of neutral lipids in cells (%) = (1 - OD value of sample group / OD value of control group) * 100%;

[0124] The results are shown in Table 4.

[0125] Table 4

[0126]

[0127] The results are shown in Table 4. The water-oil balancing composition provided in this application has a bidirectional regulatory effect on the inhibition of the TRPV1 pathway, the repair of ultraviolet damage, and the seasonal sebum secretion of SZ95 sebaceous gland cells. The sebum secretion of the water-oil balancing composition under simulated summer conditions is lower than that in winter, indicating that the water-oil balancing composition has a "seasonal oil control" effect.

[0128] The water-oil balance compositions prepared in Examples 1-7 showed a reduction rate of 45.1%-74.7% in TRPV1 protein, a reduction rate of 30.3%-58.0% in reactive oxygen species (ROS) levels, and a reduction rate of 26.1%-46.9% in neutral lipids under summer conditions; under winter conditions, the same water-oil balance compositions showed a reduction rate of 20.5%-30.4% in TRPV1 protein, a reduction rate of 6.8%-16.3% in ROS levels, and a reduction rate of -22.5% to -6.9% in neutral lipids.

[0129] The water-oil balance compositions of Examples 9-12 showed lower rates of TRPV1 protein reduction, reactive oxygen species (ROS) reduction, and neutral lipid reduction under summer and winter conditions compared to Example 1, indicating that the preparation method of Sophora flavescens root extract affects the seasonal oil-controlling properties of the compositions.

[0130] Compared with Example 1, when Comparative Examples 1-7 did not add any of the components, or when they were supplemented with other components without adding any of them, the water-oil balance compositions prepared by them did not have the same bidirectional regulatory effect on the TRPV1 pathway inhibition, UV damage repair, and seasonal sebum secretion of SZ95 sebaceous gland cells as Example 1. As can be seen from Example 1 and Comparative Examples 8-9, when the mass fraction of the components in Comparative Examples 8-9 was not within the range given in this application, the corresponding effect of this application could not be obtained. As can be seen from Example 1 and Comparative Examples 10-14, when other similar components were substituted, the corresponding effect of this application could not be obtained.

[0131] 3. In vitro experiments have demonstrated that the composition has a "seasonal moisturizing" effect by promoting the production of natural moisturizing factors.

[0132] Skin barrier function and moisturizing needs exhibit significant seasonal differences. Low temperatures and humidity in winter lead to disordered lipid arrangement in the stratum corneum, loss of natural moisturizing factors, and consequently, dry and damaged skin. Conversely, high temperatures and humidity in summer promote the degradation of filaggrin, generating natural moisturizing factors and increasing skin hydration. This experiment simulates the low temperatures and humidity of winter and the high temperatures and humidity of summer to verify the effect of the composition in specifically enhancing winter moisturizing capabilities in a 3D epidermal model.

[0133] Experimental Methods: The cell model used was a human 3D epidermal skin model (Guangdong Boxi Biotechnology Co., Ltd.). Summer testing conditions were: incubator temperature 38℃, humidity 90±5%, carbon dioxide 5±1%, UVB irradiation (20mJ / cm², wavelength 310nm); winter testing conditions were: incubator temperature 15℃, humidity 50±5%, carbon dioxide 5±1%, dark environment. 3D epidermal models were cultured and treated according to groups, followed by testing. Specifically, skin moisture content was measured using the following methods:

[0134] (1) The 3D epidermal skin model was cultured using EpiGrowth medium (Guangdong Boxi Biotechnology Co., Ltd.);

[0135] (2) In the blank control group, 12.5 μL of the cream from the blank application example was evenly applied to the surface of the epidermal model. In the sample group, 12.5 μL of the cream from application examples 1-14 and the control application examples 1-14 were applied. The samples were cultured for 24 hours in a simulated summer or winter environment.

[0136] (3) Skin moisture content detection: The skin moisture content of the 3D epidermal model was measured using a Corneometer skin moisture test probe.

[0137] The skin moisturizing ability of the composition is expressed as the increase in skin moisture content, and the calculation formula is as follows:

[0138] Increase rate of skin moisture content = (skin moisture content) 样品组 / skin moisture content 空白对照组 -1) * 100%

[0139] The results are shown in Table 5.

[0140] Table 5

[0141]

[0142] The results are shown in Table 5. The water-oil balancing compositions prepared in Examples 1-14 showed a greater increase in skin moisture content under winter conditions than under summer conditions. This indicates that the water-oil balancing compositions of this application promote the generation of natural moisturizing factors by activating filaggrin degradation in low-temperature winter environments, thereby dynamically regulating the hydration of the stratum corneum. Specifically, the obtained compositions showed an increase in skin moisture content of 9.3%-11.1% in summer and 59.9%-85.1% in winter.

[0143] The preparation method of Sophora flavescens root extract in the water-oil balance composition in Examples 9-12 was changed, and the composition had a less effective "seasonal moisturizing" effect than in Example 1, indicating that the preparation method of Sophora flavescens root extract has an impact on the moisturizing performance of the water-oil balance composition.

[0144] As can be seen from Examples 1 and Comparative Examples 1-7, the effects of this application cannot be achieved when one of the components is not added to the composition, or when other components are used to make up the difference while the component is not added. As can be seen from Examples 1 and Comparative Examples 8-9, the effects of this application cannot be achieved when the mass fraction of the components in Comparative Examples 8-9 is not within the range given in this application. As can be seen from Examples 1 and Comparative Examples 10-14, the effects of this application cannot be achieved when other similar components are used as substitutes.

[0145] Example 2

[0146] This application investigates the safety of the face creams prepared using the application examples, comparative application examples, and blank application examples, specifically by conducting human skin patch tests:

[0147] Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal volumes (0.020 mL-0.025 mL) of test samples (creams prepared for the application example, control example, and blank example) were placed in a specific patch applicator. The patch was then applied to the volunteer's arm with hypoallergenic adhesive tape, and gently pressed to ensure even application to the skin. The patch was left on for 24 hours. The blank control group used distilled water, and the blank application example used a cream without a water-oil balance composition. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 h, 24 h, and 48 h. The severity of adverse skin reactions is shown in Table 6 below.

[0148] Table 6

[0149]

[0150] The results showed that the face creams prepared in the application example, the control application example, and the blank application example all had negative reactions after human patch testing, indicating that they were safe and non-irritating to human skin.

[0151] Example 3

[0152] This application investigates the effects of face creams prepared in the following steps on the human body: (Examples include:) application examples, comparative application examples, and blank application examples.

[0153] The trial selected 162 Asian adults aged 18-60 with combination skin and self-reported skin sensitivity, and randomly divided them into 27 groups of 6 participants each. Volunteers were tested in either summer (June-August, average temperature above 28°C, average humidity above 80%) or winter (December-February, average temperature below 15°C, average humidity below 50%). The test involved applying a sample (prepared from application examples 1-14, control examples 1-12, and a blank application example) to the entire face twice daily, morning and evening. Data was collected on day 0 and day 7. After the trial, volunteers washed their faces with facial cleanser and sat quietly for 30 minutes in an air-conditioned room with a temperature of 21±1°C and humidity of 50±10%.

[0154] Researchers used a Corneometer probe to measure skin moisture content in the cheekbones and a TewaMeter®™ Hex probe to measure transepidermal water loss (TEWL) in the cheekbones. After volunteers sat still for 2 hours, researchers used a Sebumeter SM 815 probe to collect data on oil content in the forehead.

[0155] The oil-controlling effect of the composition is represented by the reduction in skin oil content, the skin moisturizing effect is represented by the improvement in skin moisture content, and the barrier repair ability is represented by the improvement in TEWL value. The formulas are as follows:

[0156] Skin oil content reduction rate = [(T0 sample group - T7 sample group) / T0 sample group * 100%] - [(T0 blank application example - T7 blank application example) / T0 blank application example * 100%];

[0157] Skin moisture content improvement rate = [(T7 sample group - T0 sample group) / T0 sample group * 100%] - [(T7 blank application case - T0 blank application case) / T0 blank application case * 100%];

[0158] TEWL value improvement rate = [(T0 sample group - T7 sample group) / T0 sample group * 100%] - [(T0 blank application example - T7 blank application example) / T0 blank application example * 100%];

[0159] The results are shown in Table 7.

[0160] Table 7

[0161]

[0162] The results are shown in Table 7. When the technical solution provided in this application is adopted, the resulting face cream has good oil control and moisturizing performance. It can precisely regulate sebum secretion without damaging the barrier under the high temperature conditions in summer, and dynamically regulate the hydration of the stratum corneum in the low temperature environment in winter. It breaks through the technical limitations of traditional products that are used in different seasons and have single and one-sided functions of active ingredients. It achieves cross-seasonal balanced care with "single formula self-adaptation" and is gentle and non-irritating, suitable for sensitive skin.

[0163] Specifically, on the one hand, the face creams obtained using Examples 1-14 showed an improvement rate of 20.1%-35.2% in skin oil content, 5.9%-6.9% in skin moisture content, and 13.7%-23.7% in TEWL value under summer conditions; on the other hand, the face creams obtained showed an improvement rate of -5.9% to -2.0% in skin oil content, 39.0%-53.6% in skin moisture content, and 8.3%-16.5% in TEWL value under winter conditions.

[0164] In Application Examples 9-12, the preparation method of the Sophora flavescens root extract in the water-oil balance composition was changed, and the composition was not as effective in oil control and moisturizing as in Application Example 1, indicating that the preparation method of Sophora flavescens root extract has an impact on the oil control and moisturizing effect of the water-oil balance composition.

[0165] As can be seen from Application Example 1 and Comparative Application Examples 1-7, the effect of this application cannot be achieved when one of the components is not added to the face cream, or when other components are used to make up the difference while the component is not added. As can be seen from Application Example 1 and Comparative Application Examples 8-9, the effect of this application cannot be achieved when the mass fraction of the component in Comparative Application Examples 8-9 is not within the range given in this application. As can be seen from Application Example 1 and Comparative Application Examples 10-14, the effect of this application cannot be achieved when other similar ingredients are used as substitutes.

[0166] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit the scope of protection of this application. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the substance and scope of the technical solutions of this application.

Claims

1. A water-oil balancing composition suitable for sensitive skin, characterized in that, The water-oil balance composition consists of active ingredients derived from medicinal Fomitopsis, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract, and Sophora flavescens root extract. The mass ratio of the active ingredient of the medicinal Fomitopsis pilosula, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (0.5~10):(0.1~5):(0.01~2):(0.01~5):(0.01~2). The active ingredients derived from medicinal fumonis include at least one of medicinal fumonis extract and medicinal fumonis-Lactobacillus plantarum bidirectional fermentation product; The method for preparing the medicinal Fertilizer of Polyporus flocculation-Lactobacillus plantarum bidirectional fermentation product includes the following steps: (1) Mix Lactobacillus plantarum culture and medicinal fibroblasts at a mass ratio of (0.5~3):1 and then anaerobic ferment. The anaerobic fermentation temperature is 37±2℃ and the anaerobic fermentation time is 48~72h to obtain the fermentation product. (2) After cooling the fermentation product to 2~6℃, it is circulated in a micro-jet homogenizer. The pressure of circulation is 1200~1400 bar and the temperature of circulation is 20~30℃. Finally, it is crushed and centrifuged, the supernatant is collected and dried to obtain the medicinal Fertilizer-Lactobacillus plantarum bidirectional fermentation product. The preparation method of the medicinal Fomitopsis thunbergii extract includes the following steps: The dried fruiting bodies of medicinal fuciformis were pulverized and passed through an 80-mesh sieve. They were then added to a 70% ethanol aqueous solution for ultrasonic extraction. The material-to-liquid ratio was 1g:30mL. The ultrasonic extraction temperature was 40℃, the power was 120W, and the time was 30min. After extraction, the mixture was centrifuged, the supernatant was collected, concentrated, and dried to obtain the medicinal fuciformis extract. The preparation method of the Sophora flavescens root extract includes the following steps: After drying and pulverizing the root of Sophora flavescens, add it to a eutectic solvent for ultrasonic extraction. After extraction, centrifuge, collect the supernatant, concentrate and dry it to obtain Sophora flavescens root extract. The eutectic solvent includes citric acid and sorbitol.

2. The water-oil balancing composition suitable for sensitive skin as described in claim 1, characterized in that, The mass ratio of the active ingredient of the medicinal fuciformis, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and sophora flavescens root extract is (1~8):(0.2~2):(0.05~0.5):(0.1~1):(0.05~0.5).

3. The water-oil balancing composition suitable for sensitive skin as described in claim 2, characterized in that, The mass ratio of the active ingredient of the medicinal Fomitopsis pilosula, 4-tert-butylcyclohexanol, hyaluronic acid, milk thistle seed extract and Sophora flavescens root extract is (3~5):(0.5~1):(0.2~0.3):(0.3~0.5):(0.1~0.2).

4. The water-oil balancing composition suitable for sensitive skin as described in claim 1, characterized in that, The method for preparing the eutectic solvent includes the following steps: mixing citric acid and sorbitol in a molar ratio of 1:(1~2), and then adding ultrapure water to obtain the eutectic solvent; in the eutectic solvent, the mass percentage of water is 15~40%.

5. The water-oil balancing composition suitable for sensitive skin as described in claim 1, characterized in that, The mass-to-volume ratio of the Sophora flavescens root to the eutectic solvent is 1 g: (20~50) mL.

6. The water-oil balancing composition suitable for sensitive skin as described in claim 1, characterized in that, The ultrasonic extraction temperature is 30~50℃, the power is 100~150W, and the time is 30~90 min.

7. The use of the water-oil balancing composition suitable for sensitive skin as described in any one of claims 1 to 6 in the preparation of skin care products.

8. The application as described in claim 7, characterized in that, The skincare products include at least one of the following: toner, lotion, cream, mask, serum, and spray.

9. A face cream, characterized in that, The face cream comprises the following components by weight percentage: 1-10% of the water-oil balancing composition as described in any one of claims 1-6, 0.1-1% of thickener, 0.5-5% of moisturizer, 0.5-5% of emulsifier, 0.01-0.3% of pH adjuster, 0.5-3% of preservative, and the balance being deionized water.

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