Traditional Chinese medicine compound composition for treating sepsis and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine compound composition for treating sepsis and a preparation method thereof. The invention provides a traditional Chinese medicine composition for treating sepsis. The traditional Chinese medicine composition comprises the following components in parts by mass: 20-25 parts of concha haliotidis, 20-25 parts of cornu bubali, 18-22 parts of radix rehmanniae recen, 18-22 parts of dogwood, 17-19 parts of radix paeoniae rubra, 15-18 parts of cortex moutan, 13-16 parts of felwort, 12-15 parts of fructus forsythiae, 9-13 parts of cyrtomium fortunei, 8-12 parts of rhizoma anemarrhenae, 8-12 parts of radix ophiopogonis, 7-11 parts of schisandra chinensis, 7-11 parts of ligusticum wallichii, 6-10 parts of pseudo-ginseng, 6-10 parts of earthworm, 5-9 parts of gynostemma pentaphyllum, 5-9 parts of poria cocos and 4-7 parts of liquorice. The composition provided by the invention achieves the technical effect of effectively treating sepsis through a triple synergistic mechanism of clearing away heat and toxic materials, promoting blood circulation to remove meridian obstruction and fixing and protecting qi and yin.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine compound composition for treating sepsis and a preparation method thereof. BACKGROUND

[0002] Sepsis is a systemic inflammatory response syndrome caused by infection, which is critical, rapidly progressive, and has characteristics of high morbidity and mortality. Modern medicine mainly adopts treatment means such as antibiotics, fluid resuscitation, and vasoactive drugs, but the clinical curative effect still has limitations, especially for patients with multiple drug-resistant bacterial infection, immune regulation imbalance, and multiple organ dysfunction, and the treatment effect is often unsatisfactory. In addition, long-term use of broad-spectrum antibiotics can easily lead to adverse reactions such as dysbacteriosis and double infection. Therefore, it is of great significance to seek a safer and more effective treatment scheme.

[0003] Traditional Chinese medicine has unique advantages in the treatment of infectious diseases and the regulation of the body's immunity. According to traditional Chinese medicine theory, sepsis belongs to the category of "warm disease", "heat toxin", and "blood syndrome", and its pathogenesis is mainly external invasion of heat toxin and internal invasion, which burns the blood, leading to burning of both qi and blood, mutual knotting of blood stasis and toxin, and even consumption of qi and yin, causing syncope. In view of this pathogenesis characteristic, traditional Chinese medicine often adopts the methods of clearing heat and resolving toxin, cooling blood and removing stasis, and tonifying qi and astringing collapse, which shows good effects in improving symptoms, regulating immunity, and protecting organ function.

[0004] At present, some traditional Chinese medicine compounds or single preparations have been used for the adjuvant treatment of sepsis, such as Xuebijing injection and Reduning injection, but their action targets are relatively single, and the overall regulation effect on complex pathogenesis needs to be improved. Therefore, it is of great clinical value and application prospect to develop a traditional Chinese medicine compound with multi-target and multi-level action characteristics to treat sepsis through the comprehensive action of clearing heat and resolving toxin, cooling blood and removing stasis, and tonifying qi and astringing collapse. SUMMARY

[0005] The present application aims to provide a traditional Chinese medicine compound composition for treating sepsis and a preparation method thereof.

[0006] In order to achieve the above-mentioned application purpose, the present application provides the following technical scheme:

[0007] The present application provides a traditional Chinese medicine composition for treating sepsis, which comprises the following components in mass fraction: 20-25 parts of concha margaritiferae, 20-25 parts of cornu bubali, 18-22 parts of radix rehmanniae, 18-22 parts of fructus corni, 17-19 parts of radix paeoniae rubra, 15-18 parts of cortex moutan, 13-16 parts of radix gentianae, 12-15 parts of forsythia suspensa, 9-13 parts of herba veronicastrae, 8-12 parts of radix anemarrhenae, 8-12 parts of radix ophiopogonis, 7-11 parts of schisandra chinensis, 7-11 parts of ligusticum wallichii, 6-10 parts of panax notoginseng, 6-10 parts of cordyceps, 5-9 parts of gynostemma pentaphyllum, 5-9 parts of poria cocos, and 4-7 parts of glycyrrhiza uralensis.

[0008] The application further provides a preparation method of the traditional Chinese medicine composition for treating sepsis.

[0009] (1) grinding the concha margaritifera usta and water buffalo horn to obtain material 1, and decocting the material 1 to obtain a decoction;

[0010] (2) grinding the notoginseng and chuanxiong to obtain material 2, and decocting the material 2 to obtain a decoction;

[0011] (3) mixing the radix rehmanniae, maianthus, red peony root, cortex moutan, gentiana, forsythia, cyrtomium fortunei, anemarrhena, ophiopogon, schisandra, giant leech, gynostemma pentaphyllum, poria cocos and liquorice to obtain material 3, and decocting the material 3 after being soaked in water to obtain a filtrate;

[0012] (4) mixing the decoction, the decoction and the filtrate, and concentrating to obtain the traditional Chinese medicine composition.

[0013] Preferably, the decocting time in step (1) is 50-70 min.

[0014] Preferably, the mass ratio of the material 1 to water in step (1) is 1:4-6.

[0015] Preferably, the decocting time in step (2) is 15-20 min.

[0016] Preferably, the mass ratio of the material 2 to water in step (2) is 1:3-4.

[0017] Preferably, the soaking time in step (3) is 1-2 h.

[0018] Preferably, the mass ratio of the material 3 to water in step (3) is 1:4-6.

[0019] Preferably, the decocting time in step (3) is 55-75 min.

[0020] Preferably, the concentration in step (3) is to 1 / 4-1 / 6 of the original volume.

[0021] Compared with the prior art, the application has the following beneficial effects:

[0022] The relationship of monarch, minister, assistant and messenger of the prescription is as follows: the monarch is Shijueming and Shuiniujiao, which dominates liver-yang and blood heat, aiming at the core pathogenesis of liver-yang hyperactivity and blood heat; the minister includes Shengdihuang, Shanzhuyu, Chishao and Danpi, which assists the monarch to nourish yin and blood, dispel stasis and consolidate the foundation, and takes into account liver-kidney yin deficiency and blood heat and stasis; the assistant is divided into three categories: Longdanca, Lianqiao and Guanzong, which clear heat and detoxify, Shuimao, Maidong and Wuyi, which nourish yin and produce fluid, Chuanqiong, Sanqi and Dilong, which activate blood and unblock collaterals, which are respectively aimed at the combined symptoms of excess fire, yin deficiency and blood stasis; the messenger is Gancao, which harmonizes various drugs, Fuling, which invigorates the spleen and removes dampness to prevent excess dampness, and Jiaoguluan, which benefits qi and detoxifies, which leads the drugs to the meridian and harmonizes the cold and warm properties of the whole prescription. The whole prescription has clear levels, but attention should be paid to the balance between bitter cold and yin nourishing to avoid damaging the middle jiao.

[0023] The composition of traditional Chinese medicine is aimed at the composition of sepsis (heat and toxin, qi and yin, blood stasis and blockage), which embodies the synergistic effect of multiple targets. Each component plays a synergistic effect through the following mechanisms. Shijueming can suppress inflammatory storm by calming liver-yang; Shuiniujiao and Shengdihuang form the core structure of "Xijiaodihuang Decoction", which can significantly reduce blood heat and toxin, inhibit the coagulation cascade and reduce microthrombosis.

[0024] Chishao and Danpi can reduce capillary permeability by clearing heat and cooling blood and activating blood and dispelling stasis; Chuanqiong can enhance the anti-microcirculation disorder effect of the former two by activating blood and regulating qi, and improve tissue ischemia and hypoxia.

[0025] Longdanca, Lianqiao and Guanzong can inhibit the release of pathogens and toxins by clearing liver and gallbladder excess fire, clearing heat and detoxifying, and resisting viral endotoxins. Shanzhuyu can prevent shock by large-dose of astringing, Wuyi can protect mitochondria by astringing qi, and Maidong can nourish yin and produce fluid, which can improve the energy metabolism disorder of sepsis. Sanqi can resolve stasis without damaging the normal, Dilong can resist coagulation by fibrinolysis, and Jiaoguluan can regulate excessive immune activation, which can improve the high coagulation state and immune paralysis of sepsis. Shuimao can astringe yin and reduce fire, which can antagonize the side effects of glucocorticoids; Fuling can invigorate the spleen and remove dampness to prevent the yin-nourishing drugs from causing excess dampness and damaging the stomach; Gancao can harmonize various drugs and enhance the anti-inflammatory activity. The composition provided by the present application can effectively treat sepsis through the triple synergistic mechanism of clearing heat and detoxifying, activating blood and unblocking collaterals, and protecting qi and yin. DETAILED DESCRIPTION

[0026] The present application provides a traditional Chinese medicine composition for treating sepsis, which comprises the following components in mass fraction: Shijueming 20-25 parts, Shuiniujiao 20-25 parts, Shengdihuang 18-22 parts, Shanzhuyu 18-22 parts, Chishao 17-19 parts, Danpi 15-18 parts, Longdanca 13-16 parts, Lianqiao 12-15 parts, Guanzong 9-13 parts, Shuimao 8-12 parts, Maidong 8-12 parts, Wuyi 7-11 parts, Chuanqiong 7-11 parts, Sanqi 6-10 parts, Dilong 6-10 parts, Jiaoguluan 5-9 parts, Fuling 5-9 parts and Gancao 4-7 parts.

[0027] Preferably, the components are as follows: 21-24 parts of Shijueming, 21-24 parts of Niu Jiao, 19-21 parts of Shengdihuang, 19-21 parts of Shanzhu, 18 parts of Chishao, 16-17 parts of Danpi, 14-15 parts of Longdanca, 13-14 parts of Lianqiao, 10-12 parts of Guanzhong, 9-11 parts of Zhimu, 9-11 parts of Maidong, 8-10 parts of Wuyi, 8-10 parts of Chuanqiong, 7-9 parts of Sanqi, 7-9 parts of Dilong, 6-8 parts of Jiaogulian, 6-8 parts of Fuling, and 5-6 parts of Gancao.

[0028] Further preferably, the components are as follows: 22-23 parts of Shijueming, 22-23 parts of Niu Jiao, 20 parts of Shengdihuang, 20 parts of Shanzhu, 18 parts of Chishao, 17 parts of Danpi, 15 parts of Longdanca, 14 parts of Lianqiao, 11 parts of Guanzhong, 10 parts of Zhimu, 10 parts of Maidong, 9 parts of Wuyi, 9 parts of Chuanqiong, 8 parts of Sanqi, 8 parts of Dilong, 7 parts of Jiaogulian, 7 parts of Fuling, and 6 parts of Gancao.

[0029] More preferably, the components are as follows: 23 parts of Shijueming, 23 parts of Niu Jiao, 20 parts of Shengdihuang, 20 parts of Shanzhu, 18 parts of Chishao, 17 parts of Danpi, 15 parts of Longdanca, 14 parts of Lianqiao, 11 parts of Guanzhong, 10 parts of Zhimu, 10 parts of Maidong, 9 parts of Wuyi, 9 parts of Chuanqiong, 8 parts of Sanqi, 8 parts of Dilong, 7 parts of Jiaogulian, 7 parts of Fuling, and 6 parts of Gancao.

[0030] The application also provides a preparation method of the traditional Chinese medicine composition for treating sepsis, which comprises the following steps:

[0031] (1) grinding Shijueming and Niu Jiao to obtain material 1, and decocting the material 1 to obtain a decoction;

[0032] (2) grinding Sanqi and Chuanqiong to obtain material 2, and decocting the material 2 to obtain a decoction;

[0033] (3) mixing Shengdihuang, Shanzhu, Chishao, Danpi, Longdanca, Lianqiao, Guanzhong, Zhimu, Maidong, Wuyi, Dilong, Jiaogulian, Fuling and Gancao to obtain material 3, and decocting the material 3 to obtain a filtrate;

[0034] (4) mixing the decoction, the decoction and the filtrate, and concentrating to obtain the traditional Chinese medicine composition.

[0035] In the application, the decoction time in step (1) is 50-70 min, preferably 55-65 min, and more preferably 60 min.

[0036] In the application, the mass ratio of the material 1 to water in step (1) is 1:4-6, and preferably 1:5.

[0037] In the application, the decoction time in step (2) is 15-20 min, preferably 16-19 min, more preferably 17-18 min, and most preferably 18 min.

[0038] In the present application, the mass ratio of the material 2 to water in step (2) is 1:3-4; preferably 1:3.

[0039] In the present application, the soaking time in step (3) is 1-2h; preferably 1.5h.

[0040] In the present application, the mass ratio of the material 3 to water in step (3) is 1:4-6; preferably 1:5.

[0041] In the present application, the decocting time in step (3) is 55-75min; preferably 60-70min; further preferably 65min.

[0042] In the present application, the concentration in step (3) is to 1 / 4-1 / 6 of the original volume; preferably 1 / 5.

[0043] The technical solutions provided by the present application will be described in detail below in conjunction with the examples, but they should not be understood as limiting the scope of protection of the present application.

[0044] Example 1

[0045] The present application provides a traditional Chinese medicine composition for treating sepsis, which is composed of the following components in mass fraction: 20 parts of stone concha, 20 parts of buffalo horn, 18 parts of raw rehmannia, 18 parts of dogwood, 17 parts of red peony root, 15 parts of dark peony root, 13 parts of gentian, 12 parts of forsythia, 9 parts of Japanese primula, 8 parts of anemarrhena, 8 parts of schisandra, 7 parts of Szechuan lovage, 6 parts of panax, 6 parts of earthworm, 5 parts of gynostemma, 5 parts of tuckahoe, and 4 parts of licorice.

[0046] The preparation method of the traditional Chinese medicine composition for treating sepsis is as follows:

[0047] (1) The stone concha and buffalo horn are crushed to obtain material 1, 4 times the mass of water is added for decocting for 50min to obtain a decocting liquid;

[0048] (2) The panax and Szechuan lovage are ground to obtain material 2, 3 times the mass of water is added for decocting for 15min to obtain a decocting liquid;

[0049] (3) The raw rehmannia, dogwood, red peony root, dark peony root, gentian, forsythia, Japanese primula, anemarrhena, earthworm, gynostemma, tuckahoe, and licorice are mixed to obtain material 3, 4 times the mass of water is added for soaking for 1h and then decocting for 55min, and a filtration liquid is obtained by filtration;

[0050] (4) The decocting liquid, decocting liquid, and filtration liquid are mixed, and concentrated to 1 / 4 of the original volume.

[0051] Example 2

[0052] The invention provides a traditional Chinese medicine composition for treating sepsis. The composition is composed of the following components in parts by mass: 25 parts of Semen Cassiae, 25 parts of Horn of Buffalo, 22 parts of Radix Rehmanniae, 22 parts of Fructus Corni, 19 parts of Red Peony Root, 18 parts of Cortex Moutan, 16 parts of Radix Gentianae, 15 parts of Fructus Forsythiae, 13 parts of Rhizoma Cyrthospermi, 12 parts of Rhizoma Anemarrhenae, 12 parts of Radix Ophiopogonis, 11 parts of Fructus Schisandrae Chinensis, 11 parts of Rhizoma Chuanxiong, 10 parts of Radix Notoginseng, 10 parts of Pheretima, 9 parts of Gynostemma Pentaphyllum, 9 parts of Poria and 7 parts of Radix Glycyrrhizae.

[0053] The preparation method of the traditional Chinese medicine composition for treating sepsis comprises the following steps:

[0054] (1) Grind Semen Cassiae and Buffalo Horn to obtain Material 1, add 6 times the weight of water and boil for 70 minutes to obtain a decoction;

[0055] (2) Grind Panax notoginseng and Chuanxiong to obtain material 2, add 4 times the weight of water and boil for 20 minutes to obtain a decoction;

[0056] (3) Mix raw Rehmannia root, Cornus officinalis, red peony root, moutan bark, gentian, forsythia, cyperus, anemarrhena, ophiopogon, schisandra, earthworm, gynostemma pentaphyllum, poria, and liquorice to obtain material 3, add 6 times the weight of water, soak for 2 hours, and then boil for 75 minutes, and filter to obtain the filtrate;

[0057] (4) The water decoction, the decoction liquid and the filtrate are mixed and concentrated to 1 / 6 of the original volume.

[0058] Example 3

[0059] The invention provides a traditional Chinese medicine composition for treating sepsis. The composition is composed of the following components in parts by mass: 23 parts of Semen Cassiae, 23 parts of Horn of Buffalo, 20 parts of Radix Rehmanniae, 20 parts of Fructus Corni, 18 parts of Radix Paeoniae Rubra, 17 parts of Cortex Moutan, 15 parts of Radix Gentianae, 14 parts of Fructus Forsythiae, 11 parts of Rhizoma Cyrtonemae, 10 parts of Rhizoma Anemarrhenae, 10 parts of Radix Ophiopogonis, 9 parts of Fructus Schisandrae Chinensis, 9 parts of Rhizoma Chuanxiong, 8 parts of Radix Notoginseng, 8 parts of Pheretima, 7 parts of Gynostemma Pentaphyllum, 7 parts of Poria and 6 parts of Radix Glycyrrhizae.

[0060] The preparation method of the traditional Chinese medicine composition for treating sepsis comprises the following steps:

[0061] (1) Grind Semen Cassiae and Buffalo Horn to obtain material 1, add 5 times the weight of water and boil for 60 minutes to obtain a decoction;

[0062] (2) Grind Panax notoginseng and Chuanxiong to obtain material 2, add 4 times the weight of water and boil for 18 minutes to obtain a decoction;

[0063] (3) Mix raw Rehmannia root, Cornus officinalis, red peony root, moutan bark, gentian, forsythia, cyperus, anemarrhena, ophiopogon, schisandra, earthworm, gynostemma pentaphyllum, poria, and liquorice to obtain material 3, add 5 times the weight of water, soak for 1.5 hours, then boil for 65 minutes, and filter to obtain a filtrate;

[0064] (4) Mix the decoction, the boiling liquid and the filtrate, and concentrate to 1 / 5 of the original volume.

[0065] Comparative Example 1, other methods are the same as Example 3, the difference is only in the replacement of the concha margaritifera and cornu bubali with equal amounts of nacre and cornu cervi.

[0066] Comparative Example 2, other methods are the same as Example 3, the difference is only in the omission of the addition of concha margaritifera and cornu bubali.

[0067] Comparative Example 3, other methods are the same as Example 3, the difference is only in the omission of the addition of corni fructus, paeoniae rubra and moutan cortex.

[0068] Experimental Example 1: Establishment of a sepsis mouse model and evaluation of traditional Chinese medicine intervention

[0069] 1. Experimental animals: 48 SPF male C57BL / 6 mice (20±2g), adaptively fed for 3 days, environmental temperature 25±2℃, humidity 60±5%, 12h light-dark cycle, free drinking and eating, bedding changed every 3 days. And according to the average weight, the mice were divided into 6 groups, and the weight difference was not significant.

[0070] 2. Grouping and treatment

[0071] Blank group (n=8): normal saline gavage

[0072] Model group (n=8): LPS (15mg / kg, ip) + normal saline gavage

[0073] Experimental group (n=8): LPS + 10g / kg of traditional Chinese medicine composition prepared in Example 3 Comparative Example 1 (n=8): LPS + 10g / kg of traditional Chinese medicine composition prepared in Comparative Example 1 Comparative Example 2 (n=8): LPS + 10g / kg of traditional Chinese medicine composition prepared in Comparative Example 2 Comparative Example 3 (n=8): LPS + 10g / kg of traditional Chinese medicine composition prepared in Comparative Example 3

[0074] Dosing regimen: LPS injection 0.5h after starting gavage, every 12h, for 5 times.

[0075] 3. Observation index

[0076] General state: record body weight and mortality every 12h.

[0077] Inflammatory factors: ELISA detection of serum IL-6, TNF-α.

[0078] Macrophage phenotype: flow cytometry detection of peritoneal macrophage F4 / 80+, CD163+, CD206+ expression. Therefore, the results are the average value, and the results are shown in the following table.

[0079] Table 1 Mouse weight investigation table (g)

[0080] Group 12h 24h 36h 48h 60h Blank group 19.2 19.4 19.5 19.3 19.6 Model group 17.5 16.6 16.0 15.7 15.3 Experimental group 18.0 17.5 17.2 16.9 17.1 Comparative example 1 17.9 17.3 16.8 16.5 16.6 Comparative example 2 17.8 17.3 16.4 16.1 15.8 Comparative example 3 17.9 17.4 16.7 16.3 16.0

[0081] The experimental results show that the mice in the blank group: the hair is smooth and shiny, the activity is active, the mental state is good, and it shows normal physiological state. After the mice in the model group were injected with LPS intraperitoneally, they showed obvious pathological reactions, including disheveled hair, loose stool, reduced activity, and a depressed mental state, which are consistent with the characteristics of the inflammation model. This indicates that the modeling in this experiment is successful.

[0082] Comparative Examples 1-3: The mice recovered to some extent compared to the model group, but the improvement was limited. Experimental group: The hair, activity, and mental state of the mice recovered most significantly, and the effect was better than that of the comparative examples. As can be seen from Table 1, the change in the body weight of the mice in the experimental group was the smallest, and the body weight increased in the later period, indicating that the use of the drug in the experimental group can improve the survival state of the mice.

[0083] Table 2 Mouse inflammation factor investigation table (pg / ml)

[0084] Group IL-6 TNF-α Blank group 15.6 18.4 Model group 35.2 244.1 Experimental group 16.8 114.8 Comparative example 1 19.7 129.7 Comparative example 2 25.2 158.6 Comparative example 3 22.7 146.2

[0085] Table 3 Mouse macrophage investigation table (%)

[0086] Group Macrophage content Blank group 76.2％ Model group 2.6％ Experimental group 25.7％ Comparative example 1 21.3％ Comparative example 2 8.7％ Comparative example 3 11.9％

[0087] Table 2 shows that the traditional Chinese medicine composition in the experimental group significantly reduced the inflammation factor level of the sepsis mice, and the effect was better than that of the other comparative example groups, especially the significant decrease in TNF-α, indicating that the prescription has a strong anti-inflammatory effect. The results of Comparative Examples 1-3 further show that the combination of core medicinal materials such as Concha Murex and buffalo horn is indispensable, and the absence or replacement will weaken the efficacy.

[0088] Table 3 shows that the macrophage content of the mice in the experimental group recovered significantly to 25.7%, which is much higher than that of the model group, indicating that the traditional Chinese medicine composition can effectively regulate immune cell function and improve the immune imbalance caused by sepsis. In contrast, the macrophage recovery degree of the comparative example groups is low, especially Comparative Example 2, which lacks the monarch drug, further verifying the scientificity of the original prescription design and the importance of synergistic effect.

[0089] The above only describes the preferred embodiments of the present application, and it should be noted that for ordinary skilled persons in the art, without departing from the principles of the present application, several improvements and refinements can be made, which should also be considered as the protection scope of the present application.

Claims

1. A Chinese medicine composition for treating sepsis, characterized in that: The invention comprises the following components in parts by weight: 20-25 parts of Semen Cassiae, 20-25 parts of Buffalo Horn, 18-22 parts of Radix Rehmanniae, 18-22 parts of Fructus Corni, 17-19 parts of Radix Paeoniae Rubra, 15-18 parts of Cortex Moutan, 13-16 parts of Radix Gentianae, 12-15 parts of Fructus Forsythiae, 9-13 parts of Rhizoma Cyrtonemae, 8-12 parts of Rhizoma Anemarrhenae, 8-12 parts of Radix Ophiopogonis, 7-11 parts of Fructus Schisandrae Chinensis, 7-11 parts of Rhizoma Chuanxiong, 6-10 parts of Radix Notoginseng, 6-10 parts of Pheretima, 5-9 parts of Gynostemma Pentaphyllum, 5-9 parts of Poria and 4-7 parts of Radix Glycyrrhizae.

2. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 1, characterized in that: The steps include: (1) Grinding Semen Cassiae and Buffalo Horn to obtain material 1, adding water and boiling to obtain a decoction; (2) Grind Panax notoginseng and Chuanxiong to obtain material 2, add water and boil to obtain a decoction; (3) Mix raw rehmannia root, cornus officinalis, red peony root, moutan bark, gentian, forsythia, cyperus, anemarrhena, ophiopogon, schisandra, earthworm, gynostemma pentaphyllum, poria, and liquorice to obtain material 3, soak in water, decoct, and filter to obtain a filtrate; (4) The water decoction, the decoction liquid and the filtrate are mixed and concentrated.

3. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The decocting time in step (1) is 50 to 70 minutes.

4. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The mass ratio of the material 1 to water in step (1) is 1:4-6.

5. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The decocting time in step (2) is 15 to 20 minutes.

6. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The mass ratio of the material 2 to water in step (2) is 1:3-4.

7. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The soaking time in step (3) is 1 to 2 hours.

8. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The mass ratio of the material 3 to water in step (3) is 1:4-6.

9. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The decocting time in step (3) is 55 to 75 minutes.

10. The method for preparing a traditional Chinese medicine composition for treating sepsis according to claim 2, characterized in that: The step (3) is concentrated to 1 / 4 to 1 / 6 of the original volume.