Ship sewage treatment system and method

Abstract

The invention relates to the technical field of ship sewage treatment, in particular to a ship sewage treatment system and method.The system comprises an intelligent sensing module which is composed of a quantum tunneling sensor array and a living microorganism sensor and used for detecting heavy metal, pathogen and chemical oxygen demand in sewage in real time; the plasma-acoustochemical synergistic reactor is configured to remove suspended matters and organic pollutants through the synergistic effect of magnetic nanoparticle adsorption and acoustochemical oxidation; the synthetic microorganism bionic reactor is internally provided with a gene editing flora and a 3D printing porous carrier and is used for degrading organic matters and removing nitrogen and phosphorus; the membrane separation and resource recovery module comprises a bionic cell membrane intelligent filtering system and an electrochemical concentration unit and is used for recycling fresh water and recovering phosphate and lithium ions; the energy recovery module is composed of a photocatalysis-microbial fuel cell and is used for realizing pollutant end treatment and synchronous generation of electric energy; and an intelligent control module.

Description

Technical Field

[0001] The present invention relates to the technical field of ship sewage treatment, and in particular to a ship sewage treatment system and method. Background Art

[0002] With the rapid development of the global shipping industry, wastewater generated by ships has caused serious pollution problems in the marine environment. Traditional wastewater treatment methods have many shortcomings in treating complex water quality and preventing the spread of pathogens, making it difficult to meet increasingly stringent environmental protection requirements. Specifically, existing technologies face the following major challenges:

[0003] Traditional sewage treatment methods usually rely on chemical precipitation, filtration and other means to remove heavy metals and pathogens, but these methods are often inefficient and prone to secondary pollution.

[0004] Traditional biological treatment methods have limited effects on the degradation of complex organic pollutants (such as petroleum hydrocarbons), especially in high salinity or low temperature environments, where microbial activity is inhibited.

[0005] Although traditional membrane separation technology can effectively separate fresh water and concentrate, it generally has problems such as low flux, high energy consumption, and easy clogging, resulting in high treatment costs.

[0006] Traditional sewage treatment processes consume high energy and lack effective energy recovery mechanisms, which leads to increased operating costs and is not conducive to sustainable development.

[0007] Traditional sewage treatment systems lack intelligent monitoring methods and are unable to respond to changes in water quality in real time, resulting in unstable treatment results and high maintenance costs.

[0008] Traditional oil pollution detection methods, such as gas chromatography-mass spectrometry (GC-MS), are highly accurate but cumbersome and time-consuming, making them inadequate for rapid online monitoring on ships. Conventional fluorescence or electrochemical sensors are susceptible to interference from complex seawater matrices (such as high salinity, humic acid, and suspended solids), and lack specificity and sensitivity for low-concentration, multi-component mixed oil pollution. In particular, existing technologies lack integrated sensing solutions that can simultaneously identify both the chemical composition of oil pollution and its biological toxicity effects (such as whether it induces the development of drug-resistant genes in pathogens). Summary of the Invention

[0009] In response to the shortcomings of the existing technology, the present invention provides a ship sewage treatment system and method that integrates intelligent sensing, multi-stage treatment, membrane separation and resource recovery, energy recovery and other functions. It not only solves many technical difficulties existing in traditional methods, but also achieves the goal of efficient, low-cost and environmentally friendly sewage treatment.

[0010] According to a first aspect of the present invention, there is provided a ship sewage treatment system comprising:

[0011] Intelligent sensing module: composed of a quantum tunneling sensor array and a living microbial sensor, used for real-time detection of heavy metals, pathogens, and chemical oxygen demand (COD) in sewage;

[0012] Multi-stage processing module:

[0013] Plasma-sonic chemical co-reactor: configured to remove suspended solids and organic pollutants through the synergistic action of magnetic nanoparticle adsorption and sonochemical oxidation;

[0014] Synthetic microbial biomimetic reactor: Built-in gene-edited bacterial flora and 3D-printed porous carriers for organic matter degradation and nitrogen and phosphorus removal;

[0015] Membrane separation and resource recovery module: includes a bionic cell membrane intelligent filtration system and an electrochemical concentration unit for freshwater resource recovery and phosphate and lithium ion recovery;

[0016] Energy recovery module: composed of a photocatalytic-microbial fuel cell, which realizes the terminal treatment of pollutants and the simultaneous generation of electricity;

[0017] Intelligent control module: Dynamically optimizes the operating parameters of each unit through the digital twin platform to ensure processing efficiency and energy balance.

[0018] This invention utilizes a quantum tunneling sensor array (graphene quantum dots surface-modified with the CRISPR-Cas12a system) and a live microbial sensor (a Vibrio fischeri mutant expressing the lux gene) to enable real-time detection and precise control of heavy metal and pathogen concentrations. Combined with the synergistic effects of magnetic nanoparticle adsorption and sonochemical oxidation, it efficiently removes suspended solids and organic pollutants, ensuring water quality safety.

[0019] This invention utilizes a synthetic microbial biomimetic reactor with a built-in genetically edited bacterial community (such as Alakanos bokum, which expresses cytochrome P450 enzymes, and Pseudomonas stutzeri, which carries an autotrophic denitrification gene cluster). This system can efficiently degrade organic pollutants and achieve nitrogen and phosphorus removal in various environments. The 3D-printed porous carrier design further enhances the microbial attachment and metabolic efficiency.

[0020] The biomimetic cell membrane intelligent filtration system in this invention utilizes a lipid bilayer membrane formed by the self-assembly of aquaporin-Z and dipalmitoylphosphatidylcholine (DPPC), combined with a carbon nanotube and polyamide hybrid matrix support layer, achieving high flux (≥100LMH / bar) and high retention rate (NaCl retention rate ≥99.9%). An electrochemical concentration unit is used to recover phosphate and lithium ions, further improving resource utilization.

[0021] The energy recovery module of the present invention is composed of a photocatalytic-microbial fuel cell, which not only realizes the terminal treatment of pollutants, but also can generate electricity synchronously (the net capacity of the system is ≥ 0.4kWh / m 3 ), significantly reducing overall energy consumption. The application of the digital twin platform ensures the dynamic optimization of the operating parameters of each unit, achieving the optimal match between processing efficiency and energy balance.

[0022] The intelligent control module of this invention uses a digital twin platform to monitor water quality data in real time and dynamically adjust the operating parameters of each unit. This intelligent management approach not only improves processing efficiency, but also reduces the need for manual intervention and maintenance costs.

[0023] According to an embodiment of the present invention, in the intelligent perception module:

[0024] The quantum tunneling sensor array is a graphene quantum dot (GQDs) surface-modified CRISPR-Cas12a system, and its detection limit for heavy metal ions is ≤0.1ppb;

[0025] The living microbial sensor is a mutant of Vibrio fischeri expressing the lux gene, and its bioluminescence intensity is linearly correlated with COD concentration in the range of 10-1000 mg / L (R 2 ≥0.99).

[0026] Vibrio fischeri mutants were designed to detect chemical oxygen demand (COD). The working principle is based on the relationship between the bioluminescence intensity of the strain and the organic matter content in the water sample. Vibrio fischeri mutants expressing the lux gene can show a corresponding bioluminescent reaction when exposed to samples containing a certain concentration of organic matter. The intensity of this reaction is linearly correlated with the chemical oxygen demand (COD) in the sample. In the range of 10-1000 mg / L, R 2 ≥0.99, so it can reflect the water quality very accurately.

[0027] When this mutant is exposed to a water sample, if biodegradable organic matter is present in the water, it will affect the bacterial metabolic rate, which in turn affects the expression level of the lux gene, resulting in a change in luminescence intensity. By quantifying this change, the COD value of the water sample can be indirectly determined.

[0028] According to an embodiment of the present invention, the plasma-sonic chemical cooperative reactor comprises:

[0029] Fe3O4@ZIF-8 core-shell structure magnetic nanoparticles, particle size 50-100nm, dosage 0.5-1g / L;

[0030] Ultrasonic generator, operating frequency is 1MHz±5%, power density is 50W / cm 2 ;

[0031] Pulse plasma device, output voltage is 20kV±5%, pulse width is 100ns±10%.

[0032] According to an embodiment of the present invention, in the synthetic microbial biomimetic reactor:

[0033] The gene-edited bacterial population includes:

[0034] Alcanivorax borkumensis expressing cytochrome P450 enzymes for petroleum hydrocarbon degradation;

[0035] Pseudomonas stutzeri carrying an autotrophic denitrification gene cluster for nitrate reduction;

[0036] The 3D printed porous carrier is a polylactic acid-glycolic acid copolymer (PLGA) porous scaffold with a surface pore size of 50±5 μm, an internal pore size of 10±2 μm, and a porosity of ≥90%.

[0037] According to an embodiment of the present invention, the bionic cell membrane intelligent filtration system includes:

[0038] The active layer is composed of a lipid bilayer membrane formed by the self-assembly of aquaporin (Aquaporin-Z protein) and dipalmitoylphosphatidylcholine (DPPC);

[0039] The support layer is a hybrid matrix of carbon nanotubes and polyamide, with a thickness of ≤200nm and an operating pressure of 0.5-1.5bar;

[0040] The water flux of the bionic cell membrane intelligent filtration system is ≥100LMH / bar, and the NaCl retention rate is ≥99.9%. Aquaporin-Z is used to construct part of the bionic cell membrane intelligent filtration system, which imitates the biological membrane mechanism in nature to achieve efficient water treatment and resource recovery.

[0041] Aquaporin-Z self-assembles with dipalmitoylphosphatidylcholine (DPPC) to form a lipid bilayer membrane, which can create an efficient selective permeable membrane for freshwater separation and concentrate treatment in wastewater treatment processes.

[0042] According to an embodiment of the present invention, the CRISPR-Cas12a system is a CdSe / ZnS core-shell structure quantum dot fixed with a CRISPR-Cas12a complex and an oil-specific DNA aptamer;

[0043] When the sensor recognizes the target pollutant, it triggers the Cas12a trans-cleavage activity, resulting in the amplification of the quantum dot fluorescence signal, and the detection limit for diesel reaches 0.01ppm.

[0044] According to an embodiment of the present invention, the particle size of the CdSe / ZnS core-shell structure quantum dots is 2-5 nm;

[0045] The CdSe / ZnS core-shell structure quantum dots are fixed on the surface through a carboxyl-amino coupling reaction.

[0046] CRISPR-Cas12a complex and oil-specific DNA aptamer.

[0047] CdSe / ZnS core-shell quantum dots have a high fluorescence quantum yield and can provide a strong and stable fluorescence signal at low concentrations. The CRISPR-Cas12a complex and oil-specific DNA aptamer were immobilized on the quantum dot surface through a carboxyl-amino coupling reaction. This surface modification method not only enhances the stability of the quantum dots but also imparts their specific recognition capabilities.

[0048] CRISPR-Cas12a complex: After recognizing a specific target sequence, the Cas12a protein triggers its trans-cleavage activity, that is, non-specifically cutting the surrounding single-stranded DNA.

[0049] Oil-specific DNA aptamers: These aptamers can specifically bind to oil pollutants such as diesel, allowing the Cas12a complex to be activated in the presence of the target pollutant.

[0050] The water sample to be tested is introduced into a detection system containing the above-mentioned quantum dot sensor. If the water sample contains oil pollutants such as diesel, the oil-specific DNA aptamer will bind to it to form a complex. The formed complex will activate the Cas12a protein, triggering its trans-cleavage activity. Cas12a non-specifically cuts the surrounding fluorescent quencher-labeled DNA probes attached to the quantum dots, resulting in a significant increase in the fluorescence signal of the quantum dots. By measuring the change in the fluorescence signal, the concentration of the oil in the water is calculated and compared with the set standard value to determine whether further treatment is needed.

[0051] When there are oily targets such as diesel in ship sewage, its specific components (such as polycyclic aromatic hydrocarbons such as pyrene and benzopyrene) bind with high affinity to DNA aptamers with unique three-dimensional structures, causing the DNA aptamer to transform from a random coil conformation to a rigid composite structure such as a G-quadruplex. The DNA aptamer-oil complex after the conformational change acts as an efficient activator, binds to the Cas12a / crRNA binary complex, induces the conformational change of the Cas12a protein, and exposes its catalytic center with trans-cleavage activity. The activated Cas12a protein indiscriminately cuts all single-stranded DNA (ssDNA) in the reaction system. The present invention pre-immobilizes a large number of ssDNA reporter molecules labeled with a fluorescence quencher group (BHQ-1) on the surface of CdSe / ZnS quantum dots through a streptavidin-biotin system. The trans-cleavage activity of Cas12a cuts off these reporter molecules, keeps the quencher group away from the quantum dot surface, thereby releasing the fluorescence resonance energy transfer (FRET) effect, resulting in exponential recovery and enhancement of the fluorescence signal of the quantum dots.

[0052] When the sensor identifies a target pollutant (such as diesel), the oil-specific DNA aptamer binds to the pollutant, triggering the trans-cleavage activity of Cas12a. Cas12a non-specifically cleaves the fluorescent quencher-labeled DNA probe attached to the surface of the quantum dots, resulting in a significant enhancement of the quantum dot fluorescence signal. Due to this signal amplification effect, the sensor's detection limit for diesel can reach 0.01 ppm (parts per million), far exceeding the sensitivity of traditional detection methods.

[0053] In ship sewage treatment systems, this sensor can monitor the oil content in wastewater in real time, ensuring that the treated water meets environmental standards. It can also be used for marine environmental monitoring, quickly and accurately detecting oil contamination in water bodies and enabling timely response measures.

[0054] According to an embodiment of the present invention, the electrochemical concentration unit uses an IrO2-Ta2O5 / Ti anode and a nitrogen-doped graphene cathode.

[0055] The anode's iridium oxide and tantalum oxide coatings exhibit excellent electrocatalytic activity and stability, enabling long-term operation at high current densities without significant degradation. Titanium, as a base material, provides both excellent mechanical strength and corrosion resistance, making it suitable for use in harsh environments such as those with strong acids and bases.

[0056] The cathode uses nitrogen-doped graphene. Nitrogen atoms doped into the graphene structure introduce more active sites, enhancing electrocatalytic activity, particularly in reduction reactions. Nitrogen doping also improves the conductivity and hydrophilicity of graphene, promoting ion transport in the electrolyte.

[0057] According to a second aspect of the present invention, a method for treating ship sewage is provided, which uses the above-mentioned ship sewage treatment system and comprises the following steps:

[0058] The concentration of pollutants in sewage is detected through the intelligent sensing module, and the data is transmitted to the intelligent control module in real time;

[0059] In a plasma-sonic chemical co-reactor, heavy metals are adsorbed by magnetic nanoparticles, while organic matter is degraded by reactive oxygen species generated by ultrasonic cavitation and plasma activation.

[0060] In a synthetic microbial biomimetic reactor, biodegradation was carried out by gene-edited bacterial consortia, and the hydraulic retention time (HRT) was controlled at 4 ± 0.5 h;

[0061] The bionic cell membrane intelligent filtration system separates fresh water and concentrate, the fresh water is reused, and the concentrate enters the electrochemical unit to recover phosphate and lithium ions;

[0062] Residual pollutants are treated by photocatalysis-microbial fuel cells and converted into electrical energy. The net capacity of the system is ≥0.4kWh / m 3 ;

[0063] The parameters of each module are dynamically adjusted through the digital twin platform to ensure that the discharged water quality is lower than 50% of the International Maritime Organization (IMO) limit.

[0064] According to an embodiment of the present invention, the recovery rate of the magnetic nanoparticles is ≥98%, and the recovered magnetic nanoparticles are regenerated with a 0.1M citric acid solution;

[0065] The pH value of the sonochemical oxidation stage was maintained at 3-5 by an automatic dosing system, and the hydroxyl radical (·OH) concentration was ≥1×10 -4 mol / L.

[0066] According to an embodiment of the present invention, in step (4):

[0067] Current density is 5-20mA / cm 2 ;

[0068] Phosphate recovery rate ≥ 95%, lithium ion recovery rate ≥ 90%.

[0069] The sewage treatment system and method provided by the present invention effectively remove heavy metals and pathogens, ensuring water quality safety through advanced sensing technology and collaborative processing methods. Gene-edited bacterial communities and 3D-printed porous carriers are used to efficiently degrade organic pollutants, improving biodegradation efficiency. A bionic cell membrane intelligent filtration system and electrochemical concentration unit are used to achieve efficient water resource utilization and resource recovery. Pollutant treatment and energy recovery are achieved through photocatalytic-microbial fuel cells, reducing overall energy consumption. A digital twin platform is used to achieve real-time monitoring and dynamic adjustment, improving system stability and operability.

[0070] This invention innovatively constructs a dual-mode chemical-biological sensing interface based on the synergistic amplification of the trans-cleavage activity of CRISPR-Cas12a and the energy transfer effect of quantum dots. This interface is more than a simple superposition of CRISPR and quantum dots. Instead, through sophisticated molecular design, it achieves highly specific recognition and signal conversion of oil molecules, ultimately enabling ultrasensitive detection through a significant enhancement of the quantum dot fluorescence signal.

[0071] The present invention provides a ship sewage treatment system and treatment method that integrates multiple advanced technologies, which not only solves many technical difficulties existing in traditional methods, but also achieves the goals of efficient, low-cost and environmentally friendly sewage treatment. DETAILED DESCRIPTION

[0072] The embodiments of the present application are based on a ship sewage treatment system and method, providing a complete ship sewage treatment process.

[0073] Example 1

[0074] The system includes an intelligent sensing module, a multi-stage processing module, a membrane separation and resource recovery module, an energy recovery module and an intelligent control module.

[0075] 1. Intelligent perception module

[0076] Sensor installation and calibration:

[0077] A quantum tunneling sensor array (graphene quantum dots (GQDs) surface-modified with the CRISPR-Cas12a system) and a living microbial sensor (a Vibrio fischeri mutant expressing the lux gene) were installed at the ship's sewage inlet.

[0078] The sensor was calibrated to ensure that the detection limit of heavy metal ions was ≤0.1ppb and the COD concentration detection range was linearly correlated within 10-1000mg / L (R 2 ≥0.99).

[0079] Real-time monitoring:

[0080] Sensors are activated to monitor heavy metals, pathogens, and chemical oxygen demand (COD) in sewage in real time, and transmit the data to the central control system.

[0081] The central control system adjusts the parameters of subsequent processing steps based on real-time data.

[0082] 2. Multi-level processing module

[0083] Plasma-sonic chemical co-reactor:

[0084] The sewage was introduced into a plasma-sonic chemical collaborative reactor, and Fe3O4@ZIF-8 core-shell structured magnetic nanoparticles (particle size of 50-100 nm, dosage of 0.5-1 g / L) were added.

[0085] Start the ultrasonic generator (operating frequency is 1MHz±5%, power density is 50W / cm 2 ) and a pulsed plasma device (output voltage of 20kV±5%, pulse width of 100ns±10%).

[0086] Magnetic nanoparticles are used to adsorb heavy metals in wastewater, and organic pollutants are degraded by reactive oxygen species generated by ultrasonic cavitation and plasma activation.

[0087] The recovery rate of magnetic nanoparticles is ≥98%. After recovery, they are regenerated with 0.1M citric acid solution for next use.

[0088] The pH value of the sonochemical oxidation stage was maintained at 3-5 by an automatic dosing system, and the hydroxyl radical (·OH) concentration was ≥1×10 -4 mol / L.

[0089] Synthetic microbial biomimetic reactor:

[0090] The wastewater treated by the plasma-sonic chemical co-reactor is introduced into the synthetic microbial biomimetic reactor.

[0091] The reactor is equipped with a built-in gene-edited bacterial community (such as A. borkumensis expressing cytochrome P450 enzymes and Pseudomonas stutzeri carrying an autotrophic denitrification gene cluster), attached to a 3D-printed PLGA porous scaffold (surface pore size of 50±5μm, internal pore size of 10±2μm, and porosity ≥90%).

[0092] The hydraulic retention time (HRT) was controlled at 4±0.5 hours, and the gene-edited bacterial community was used to degrade organic matter and remove nitrogen and phosphorus.

[0093] 3. Membrane separation and resource recovery module

[0094] Bionic cell membrane intelligent filtration system:

[0095] The biodegradable sewage is introduced into the bionic cell membrane intelligent filtration system.

[0096] The system consists of a lipid bilayer membrane formed by self-assembly of Aquaporin-Z protein and dipalmitoylphosphatidylcholine (DPPC) as the active layer, and a carbon nanotube and polyamide hybrid matrix as the supporting layer (thickness ≤ 200nm, operating pressure 0.5-1.5bar).

[0097] The filtration system has high flux (≥100LMH / bar) and high retention rate (NaCl retention rate ≥99.9%), achieving separation of fresh water and concentrate.

[0098] Electrochemical concentration unit:

[0099] The concentrated solution enters the electrochemical concentration unit, using IrO2-Ta2O5 / Ti anode and nitrogen-doped graphene cathode, with a current density of 5-20mA / cm 2 .

[0100] Under the action of the electric field, phosphate and lithium ions migrate to the corresponding electrodes and accumulate or precipitate near the electrodes.

[0101] The phosphate recovery rate is ≥95%, and the lithium ion recovery rate is ≥90%. These valuable substances are collected and stored for reuse.

[0102] 4. Energy recovery module

[0103] Photocatalytic-microbial fuel cells:

[0104] Residual pollutants are introduced into photocatalytic-microbial fuel cells, and the pollutants are converted into electrical energy by utilizing the synergistic effect of photocatalysts and microorganisms.

[0105] System net capacity ≥ 0.4kWh / m 3 The generated electricity can be used to support the operation of the entire sewage treatment system and reduce energy consumption costs.

[0106] 5. Intelligent control module

[0107] Digital Twin Platform:

[0108] Use the digital twin platform to monitor the operating status and water quality parameters of each module in real time, and dynamically optimize the operating parameters of each unit.

[0109] Through data analysis and feedback mechanisms, the discharged water quality is ensured to be below 50% of the International Maritime Organization (IMO) limit.

[0110] Specific implementation steps

[0111] Startup and preprocessing:

[0112] Install and calibrate all sensors and processing equipment to ensure the system is in optimal working condition.

[0113] Turn on the intelligent sensing module to monitor various indicators in sewage in real time.

[0114] Start all modules, preheat and calibrate sensors;

[0115] Input ship sewage and record initial water quality data.

[0116] The intelligent sensing module records the sensor output signal (fluorescence intensity, luminous intensity);

[0117] Compare with the standard curve to verify the accuracy of sensor response;

[0118] Output the pollutant type and concentration prediction results.

[0119] Primary treatment: plasma-sonic chemical synergistic reaction:

[0120] The sewage is introduced into the plasma-sonochemical co-reactor, magnetic nanoparticles are added, and the ultrasonic generator and plasma device are started.

[0121] Run the reactor and set the ultrasonic frequency (1 MHz), power density (50 W / cm 2 ), pulse plasma parameters (20 kV, 100 ns);

[0122] Fe3O4@ZIF-8 magnetic nanoparticles (0.5–1 g / L) were added;

[0123] Monitor and adjust pH to ensure the required hydroxyl radical concentration.

[0124] Take samples regularly to test the removal rates of heavy metals, COD, and petroleum substances.

[0125] The magnetic nanoparticles are recovered and regenerated for the next round of use.

[0126] Secondary treatment: synthetic microbial biomimetic reactions:

[0127] The sewage that has undergone primary treatment is introduced into a synthetic microbial biomimetic reactor, and gene-edited bacterial communities are used for biodegradation and denitrification and phosphorus removal, with the HRT controlled at 4±0.5 hours; changes in ammonia nitrogen and total phosphorus are monitored.

[0128] Control the hydraulic retention time to ensure that the treatment effect reaches the expected target.

[0129] Tertiary treatment: membrane separation and resource recovery:

[0130] The biodegradable wastewater was introduced into the bionic cell membrane intelligent filtration system, the operating pressure was set (0.5–1.5 bar), and the membrane flux was recorded;

[0131] Analyze the water quality (pH, conductivity, NaCl retention rate) on the fresh water side and separate the fresh water and concentrate.

[0132] The concentrated solution enters the electrochemical concentration unit and the current density is adjusted (5–20 mA / cm 2 ), selectively recover phosphate and lithium ions, collect phosphate precipitate and lithium ion enriched solution, and calculate the recovery rate (PO43-≥95%, Li+≥90%).

[0133] Four-stage treatment: Energy recovery:

[0134] The remaining pollutants are introduced into the photocatalytic-microbial fuel cell, the photocatalytic-MFC is operated, the open circuit voltage and short circuit current are measured, and they are converted into electrical energy for the system itself or other purposes.

[0135] Intelligent optimization and monitoring:

[0136] The digital twin platform monitors the system operation status in real time and dynamically adjusts the parameters of each module to ensure processing efficiency and energy balance.

[0137] Regularly maintain and calibrate each sensor and processing equipment to ensure long-term stable operation of the system.

[0138] Experimental example

[0139] Verify the removal effect of various pollutants (such as COD, BOD, heavy metals, nitrogen and phosphorus, etc.) of the ship sewage treatment system based on intelligent sensing, multi-stage treatment, membrane separation and resource recovery, and energy recovery, and evaluate its resource utilization and energy recovery capabilities.

[0140] Sewage type: simulated mixture of ship domestic sewage + oily wastewater + a small amount of industrial wastewater. Reference values ​​of initial water quality parameters:

[0141] COD: 500–1000 mg / L

[0142] BOD5: 200–400 mg / L

[0143] Ammonia nitrogen (NH3-N): 30–60 mg / L

[0144] Total phosphorus (TP): 5–15 mg / L

[0145] Heavy metals (such as Cu 2+ 、Zn 2+ , Pb 2+ ): 0.1–1.0 mg / L

[0146] Petroleum substances (diesel): 5–50ppm

[0147] After the sewage was treated using the sewage treatment system and sewage treatment method provided in Example 1, the treated sewage was tested. The testing method is shown in Table 1:

[0148] Table 1. Detection methods for Example 1

[0149]

[0150] The above method was used to measure the sewage treated by the system and method of Example 1. The results are shown in Table 2.

[0151] Table 2. Test results of Example 1

[0152] project Test results COD removal rate ≥95％ Ammonia nitrogen removal rate ≥90％ Total phosphorus removal rate ≥85％ Heavy metal removal rate ≥99％ Oil removal rate ≥98％ <![CDATA[PO43- recovery rate]]> ≥95％ <![CDATA[Li + Recovery rate]]> ≥90％ Fresh water production rate ≥80％ System net capacity <![CDATA[≥0.4kWh / m 3 ]]> Intelligent control response time ≤5 minutes System continuous stable operation cycle ≥72 hours

[0153] The sewage treatment system and method provided by the present invention effectively remove heavy metals and pathogens, ensuring water quality safety through advanced sensing technology and collaborative processing methods. Gene-edited bacterial communities and 3D-printed porous carriers are used to efficiently degrade organic pollutants, improving biodegradation efficiency. A bionic cell membrane intelligent filtration system and electrochemical concentration unit are used to achieve efficient water resource utilization and resource recovery. Pollutant treatment and energy recovery are achieved through photocatalytic-microbial fuel cells, reducing overall energy consumption. A digital twin platform is used to achieve real-time monitoring and dynamic adjustment, improving system stability and operability.

[0154] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0155] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A ship sewage treatment system, characterized in that: Includes the following connected in sequence: Intelligent sensing module: composed of a quantum tunneling sensor array and a live microbial sensor, used for real-time detection of heavy metals, pathogens, and chemical oxygen demand in sewage. The quantum tunneling sensor array is a graphene quantum dot surface-modified with a CRISPR-Cas12a system. The CRISPR-Cas12a system is a CdSe / ZnS core-shell quantum dot immobilized with a CRISPR-Cas12a complex and an oil-specific DNA aptamer. The live microbial sensor is a Vibrio fischeri mutant expressing the lux gene. Multi-stage processing module, including: Plasma-sonic chemical co-reactor: configured to remove suspended solids and organic pollutants through the synergistic action of magnetic nanoparticle adsorption and sonochemical oxidation; Synthetic microbial biomimetic reactor: Built-in gene-edited bacterial colonies and 3D-printed porous carriers for organic matter degradation and nitrogen and phosphorus removal. The gene-edited bacterial colonies include: Alakannoides bokum expressing cytochrome P450 enzymes for petroleum hydrocarbon degradation; Pseudomonas stutzeri carrying an autotrophic denitrification gene cluster for nitrate reduction; The 3D printed porous carrier is a poly(lactic acid-co-glycolic acid) porous scaffold; Membrane separation and resource recovery module: includes a bionic cell membrane intelligent filtration system and an electrochemical concentration unit for freshwater resource recovery and phosphate and lithium ion recovery; Energy recovery module: composed of a photocatalytic-microbial fuel cell, which realizes the terminal treatment of pollutants and the simultaneous generation of electricity; Intelligent control module: Dynamically optimizes the operating parameters of each unit through the digital twin platform to ensure processing efficiency and energy balance.

2. The system according to claim 1, wherein: In the intelligent perception module: The bioluminescence intensity of the living microorganism sensor is linearly correlated with the COD concentration in the range of 10-1000 mg / L.

3. The system according to claim 1, wherein: The plasma-sonic chemical cooperative reactor comprises: Fe3O4@ZIF-8 core-shell structure magnetic nanoparticles, particle size 50-100nm, dosage 0.5-1g / L; Ultrasonic generator, operating frequency is 1MHz±5%, power density is 50W / cm 2 ; Pulse plasma device, output voltage is 20kV±5%, pulse width is 100ns±10%.

4. The system according to claim 1, wherein: In the synthetic microbial biomimetic reactor: The surface pore size of the 3D printed porous carrier is 50±5 μm, the internal pore size is 10±2 μm, and the porosity is ≥90%.

5. The system according to claim 1, wherein: The bionic cell membrane intelligent filtration system comprises: The active layer is composed of a lipid bilayer membrane formed by the self-assembly of aquaporin and dipalmitoylphosphatidylcholine; The support layer is a hybrid matrix of carbon nanotubes and polyamide, with a thickness of ≤200nm and an operating pressure of 0.5-1.5bar; The water flux of the bionic cell membrane intelligent filtration system is ≥100LMH / bar.

6. The system according to claim 1, wherein: When the quantum tunneling sensor array identifies the target pollutant, it triggers the Cas12a trans-cleavage activity, resulting in the amplification of the quantum dot fluorescence signal, and the detection limit for diesel reaches 0.01 ppm.

7. The system according to claim 6, characterized in that The particle size of the CdSe / ZnS core-shell structure quantum dots is 2-5 nm; The CdSe / ZnS core-shell structure quantum dots are surface-fixed with a CRISPR-Cas12a complex and an oil stain-specific DNA aptamer through a carboxyl-amino coupling reaction.

8. The system according to claim 1, wherein: The electrochemical concentration unit adopts an IrO2-Ta2O5 / Ti anode and a nitrogen-doped graphene cathode.

9. A method for treating ship sewage, using the ship sewage treatment system according to any one of claims 1 to 8, comprising the following steps: The concentration of pollutants in sewage is detected through the intelligent sensing module, and the data is transmitted to the intelligent control module in real time; In a plasma-sonic chemical co-reactor, heavy metals are adsorbed by magnetic nanoparticles, while organic matter is degraded by reactive oxygen species generated by ultrasonic cavitation and plasma activation. In a synthetic microbial biomimetic reactor, biodegradation was carried out by gene-edited bacterial communities, and the hydraulic retention time was controlled at 4±0.5 hours; The bionic cell membrane intelligent filtration system separates fresh water and concentrate, the fresh water is reused, and the concentrate enters the electrochemical unit to recover phosphate and lithium ions; Residual pollutants are treated by photocatalysis-microbial fuel cells and converted into electrical energy. The net capacity of the system is ≥0.4kWh / m 3 ; The parameters of each module are dynamically adjusted through the digital twin platform to ensure that the discharged water quality is lower than 50% of the International Maritime Organization limit.

10. The method according to claim 9, characterized in that The recovery rate of the magnetic nanoparticles is ≥98%, and the recovered magnetic nanoparticles are regenerated with 0.1M citric acid solution; The pH value of the sonochemical oxidation stage was maintained at 3-5 by an automatic dosing system, and the hydroxyl radical concentration was ≥1×10 -4 mol / L.

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