A microfluidic nucleic acid detection device

Abstract

This invention relates to a microfluidic nucleic acid detection device, belonging to the field of detection, comprising a base plate, a chip inlet / outlet module mounted on one side above the base plate, a chip stage mounted on the chip inlet / outlet module and carrying a microfluidic chip with a liquid sac, a displacement module mounted on the chip stage, a crushing module mounted on the displacement module and squeezing liquid from the liquid sac into the microfluidic chip, a pipetting module mounted on one side of the crushing module and pumping the liquid flow within the microfluidic chip, a pneumatic module mounted above the front end of the base plate and controlling the opening and closing of the microfluidic chip's flow channel, a heating module mounted on the other side above the base plate and locally heating the microfluidic chip and self-checking its temperature, and a fluorescence module for collecting fluorescence signals from the microfluidic chip. This invention has the advantage of being able to quickly detect different types of microfluidic chips and obtain accurate results.

Description

Technical Field

[0001] This invention relates to the field of detection equipment technology, and in particular to a microfluidic nucleic acid detection device. Background Technology

[0002] Microfluidic chip technology refers to the science and technology involved in systems that use microchannels to process or manipulate tiny fluids. It is an emerging interdisciplinary field involving chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Due to their miniaturization and integration characteristics, microfluidic devices are often called microfluidic chips, and are also known as lab-on-a-chip systems and micrototal analysis systems.

[0003] Currently, nucleic acid extraction and purification, nucleic acid amplification, and nucleic acid hybridization are all performed separately, requiring instruments such as sample preparation instruments, nucleic acid extractors, and PCR instruments for amplification. The entire nucleic acid testing process mainly includes the following steps: manually extracting samples or manually adding samples to a fully automated nucleic acid extractor to extract and purify nucleic acids; manually transferring the purified nucleic acid solution to a nucleic acid amplification instrument for amplification; and finally transferring the amplified products to a fully automated analyzer. The entire testing process requires complex and bulky equipment, resulting in low efficiency, poor flexibility, and high equipment costs. This makes the entire operation cumbersome and requires highly skilled personnel. Furthermore, because the testing requires skilled technicians, portable testing for home or other locations is not feasible.

[0004] Therefore, to address the above shortcomings, a microfluidic nucleic acid detection device is needed. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] The technical problem to be solved by this invention is to address the issues of cumbersome, highly specialized, and low universality of conventional testing procedures.

[0007] (II) Technical Solution

[0008] To address the aforementioned technical problems, this invention provides a microfluidic nucleic acid detection device, comprising a base plate, a chip inlet / outlet module mounted on one side above the base plate, a chip stage mounted on the chip inlet / outlet module and carrying a microfluidic chip with a liquid sac, a displacement module mounted on the chip stage, a crushing module mounted on the displacement module and squeezing liquid from the liquid sac into the microfluidic chip, a pipetting module mounted on one side of the crushing module and pumping the liquid flow within the microfluidic chip, a pneumatic module mounted above the front end of the base plate and controlling the opening and closing of the microfluidic chip's flow channels, a heating module mounted on the other side above the base plate and locally heating the microfluidic chip and self-checking its temperature, and a fluorescence module for acquiring fluorescence signals from the microfluidic chip.

[0009] As a further explanation of the present invention, preferably, the heating module includes a heating moving motor, a heating moving screw, a heating moving connecting seat, and a heating platform. The heating moving motor is fixedly connected to the top of the base plate, the heating moving screw is rotatably connected to the output end of the heating moving motor, the heating moving connecting seat is threadedly connected to the heating moving screw, and a slide rail slider with the same axial direction as the heating moving screw is connected to one side of the heating moving connecting seat to prevent the heating moving connecting seat from deflecting when it moves. The heating platform is slidably connected to the heating moving connecting seat and moves up and down. The heating platform is provided with heating elements to control the temperature between 60°C and 95°C.

[0010] As a further explanation of the present invention, preferably, the heating movable connecting seat has a plurality of vertical channels, a heating plate is provided at the bottom of the channel, a memory spring made of composite shape memory polymer material is fixedly connected to the heating plate, a cylindrical sliding column is abutted on the memory spring, and the top of the sliding column extends out of the heating movable connecting seat and is fixedly connected to the heating platform.

[0011] As a further explanation of the present invention, preferably, the heating elements in different channels have different heating temperatures, with a heating range of 60°C to 95°C; the memory springs in different channels all contain carbon fiber material to enhance stress, and the memory springs elongate by 2 cm when heated to 60°C.

[0012] As a further explanation of the present invention, preferably, square hollowed-out upright plates are fixedly connected to the left and right sides of the base plate, and the chip platform is a plate-shaped structure with several square holes or slots. The left and right sides of the chip platform are slidably connected to the two upright plates through slide rail sliders.

[0013] As a further explanation of the present invention, preferably, the chip entry / exit module includes an entry / exit motor, an entry / exit screw, and a connecting frame. The entry / exit motor is fixedly connected to the right side upright plate, the entry / exit screw is rotatably connected to the output end of the entry / exit motor, and the connecting frame is threadedly connected to the outside of the entry / exit screw. The connecting frame is fixedly connected to the chip stage to control the chip stage to move back and forth in the horizontal direction.

[0014] As a further explanation of the present invention, preferably, the base plate and the upright plate are covered with an outer shell, which encloses the chip entry / exit module, chip stage, displacement module, crushing module, liquid pipetting module, pneumatic module, heating module and fluorescence module to form a black box inside the outer shell; the front end of the outer shell has an inlet / outlet so that the chip stage extends out of the outer shell, and the inlet / outlet is hinged with a door to close the outer shell.

[0015] As a further explanation of the present invention, preferably, the displacement module includes a displacement motor, a displacement screw, and a displacement platform. The displacement motor is fixedly connected to the top right side of the upright plate, the displacement screw is rotatably connected to the output end of the displacement motor, the axis of the displacement screw is horizontal in the left-right direction, the displacement platform is threadedly connected to the outside of the displacement screw and is connected to a slide rail slider in the same direction as the axis of the displacement screw to prevent the displacement platform from deflecting when it moves, and the crushing module is mounted on the displacement platform.

[0016] As a further explanation of the present invention, preferably, the pipetting module is mounted on the top left side of the upright plate and is on the same horizontal line as the displacement module. The pipetting module includes a pipetting motor, a pipetting screw, and a pipetting head. The pipetting motor is fixedly connected to the upright plate, and the pipetting screw is rotatably connected to the output end of the pipetting motor. The length direction of the pipetting screw is vertical. The pipetting head is threadedly connected to the pipetting screw and is connected to a smooth rod in the same direction as the axis of the pipetting screw to prevent the pipetting head from deflecting when it moves. A gas tube is connected to one side of the pipetting head to extract the gas in the flow channel of the microfluidic chip.

[0017] As a further explanation of the present invention, preferably, the pneumatic module includes a pneumatic motor, a longitudinal screw, a pneumatic bracket, and air ports. The pneumatic motor is fixedly connected to the top of the base plate, the longitudinal screw is rotatably connected to the output end of the pneumatic motor, the middle part of the pneumatic bracket is threadedly connected to the longitudinal screw, and both ends of the pneumatic bracket are inserted with smooth rods in the same direction as the longitudinal screw. The smooth rods are fixedly connected to the upright plate to limit the deflection of the pneumatic bracket. Several air ports are fixedly connected to the pneumatic bracket at intervals, and each air port is connected to an air pipe below it. Liquid flow is controlled by passing air into different air pipes.

[0018] (III) Beneficial Effects

[0019] The above-described technical solution of the present invention has the following advantages:

[0020] This invention designs a small, lightweight testing instrument suitable for real-time nucleic acid sample testing in a wider range of environments, such as community hospitals, homes, schools, and customs. It is also simple to operate; the instrument automatically outputs results after a single sample addition, requiring no professional personnel. After sample addition, the nucleic acid testing device automates the entire process of high-throughput nucleic acid testing and pathogen screening, including nucleic acid extraction, amplification, and detection. Attached Figure Description

[0021] Figure 1 This is an assembly rendering of the present invention;

[0022] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0023] Figure 3 This is the left rear view of the present invention;

[0024] Figure 4 This is the right rear view of the present invention;

[0025] Figure 5 This is a top view of the present invention;

[0026] Figure 6 This is a structural diagram of the heating movable connecting seat of the present invention;

[0027] Figure 7 This is an internal configuration diagram of the heating movable connecting seat of the present invention.

[0028] In the diagram: 1. Base plate; 11. Vertical plate; 12. Fan; 13. Housing; 14. Inlet / outlet; 15. Screen; 16. Printer; 2. Chip input / output module; 21. Input / output motor; 22. Input / output screw; 23. Connecting frame; 3. Chip stage; 4. Displacement module; 41. Displacement motor; 42. Displacement screw; 43. Displacement stage; 5. Crushing module; 51. Crushing motor; 52. Squeezing head; 6. Pipetting module; 61. Pipetting motor; 62. Pipetting screw; 63. Transfer... 7. Liquid head; 8. Pneumatic module; 9. Pneumatic motor; 10. Longitudinal screw; 11. Pneumatic support; 2. Air inlet; 3. Heating module; 4. Heating moving motor; 5. Heating moving screw; 6. Heating moving connector; 7. Heating plate; 8. Memory spring; 9. Sliding column; 10. Wire sleeve; 11. Heating platform; 12. Fluorescent module; 13. Fluorescent moving motor; 14. Fluorescent moving screw; 15. Fluorescent moving connector; 16. Fluorescent sensor. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] A microfluidic nucleic acid detection device, combined with Figure 2 , Figure 3 It includes a base plate 1, a chip inlet / outlet module 2 mounted on one side above the base plate 1, a chip stage 3 mounted on the chip inlet / outlet module 2 and carrying a microfluidic chip with a liquid bladder, a displacement module 4 mounted on the chip stage 3, a crushing module 5 mounted on the displacement module 4 and squeezing the liquid in the liquid bladder into the microfluidic chip, a pipetting module 6 mounted on one side of the crushing module 5 and pumping the liquid flow in the microfluidic chip, a pneumatic module 7 mounted above the front end of the base plate 1 and controlling the opening and closing of the microfluidic chip channel, a heating module 8 mounted on the other side above the base plate 1 and locally heating the microfluidic chip and self-checking the temperature, and a fluorescence module 9 for collecting the fluorescence signal of the microfluidic chip.

[0031] Combination Figure 1 , Figure 2 The base plate 1 has square, perforated upright plates 11 fixed to its left and right sides to secure various modules. Fans 12 are fixed to the perforated sections of the upright plates 11 to dissipate heat from the modules. The base plate 1 and upright plates 11 are covered by an outer shell 13, which encloses the chip entry / exit module 2, chip stage 3, displacement module 4, crushing module 5, pipetting module 6, pneumatic module 7, heating module 8, and fluorescence module 9, creating a black box inside the shell 13. An inlet / outlet 14 is located at the front of the shell 13, allowing the chip stage 3 to extend beyond it by moving along a slide rail. A microfluidic chip containing the sample to be tested can then be placed on the chip stage 3, which retracts into the shell 13 for self-detection. Doors are hinged to the inlet / outlet 14 to close the shell 13, preventing external light from entering and affecting fluorescence detection. A screen 15 is also provided on the shell 13 for selecting the type of microfluidic chip and the type of sample to be tested, enabling the detection device to perform corresponding control operations based on the input information. The screen 15 can also display the working time and results. A printer 16 is also fixed to the top of the outer casing 13 for printing out the test results, so that users can view and keep them.

[0032] Combination Figure 2 , Figure 3 The chip entry / exit module 2 includes an entry / exit motor 21, an entry / exit screw 22, and a connecting frame 23. The entry / exit motor 21 is fixedly connected to the right upright plate 11. The entry / exit screw 22 is rotatably connected to the output end of the entry / exit motor 21. The connecting frame 23 is threadedly connected to the outside of the entry / exit screw 22 and is fixedly connected to the chip platform 3. The left and right sides of the chip platform 3 are slidably connected to the two upright plates 11 through slide rails and sliders so that the connecting frame 23 can control the chip platform 3 to move back and forth in the horizontal direction, so that the chip platform 3 can automatically extend and retract into the outer shell 13 without manual operation by the user.

[0033] Combination Figure 2 , Figure 3 The chip platform 3 is a plate-like structure with several square holes or slots. Various liquid sacs can be spaced along the horizontal line on the microfluidic chip, which can respectively store liquids such as lysis buffer, eluent, and cleaning solution. Users can choose different types according to their needs. The ports of the liquid sacs, pneumatic valves, and pipetting ports are all located at the square holes or slots so that other modules can contact them to automatically control the flow of liquids within the microfluidic chip.

[0034] Combination Figure 2 , Figure 3The displacement module 4 includes a displacement motor 41, a displacement screw 42, and a displacement platform 43. The displacement motor 41 is fixed to the top right side of the upright plate 11. The displacement screw 42 is rotatably connected to the output end of the displacement motor 41. The axis of the displacement screw 42 is horizontal in the left-right direction. The displacement platform 43 is threaded to the outside of the displacement screw 42 and is connected to a slide rail slider in the same direction as the axis of the displacement screw 42 to prevent the displacement platform 43 from deflecting when it moves. The crushing module 5 is mounted on the displacement platform 43. Based on the data input from the microfluidic chip, the system calls the database built into the detection device to query and find the corresponding start / stop data. Then, based on this data, the system controls the displacement motor 41 to rotate and stop at a specified time. At this time, the crushing module 5 can be moved above the liquid bladder.

[0035] Combination Figure 2 , Figure 3 The crushing module 5 includes a crushing motor 51 and a crushing head 52. The crushing motor 51 is fixedly connected to the displacement stage 43. The output end of the crushing motor 51 is threadedly connected to the middle of the crushing head 52 via a screw. The crushing head 52 is slidably connected to the displacement stage 43 around its perimeter via a smooth rod. When the displacement motor 41 stops rotating, the controller built into the detection device controls the crushing motor 51 to start, causing the crushing head 52 to move downwards and contact the liquid sac on the microfluidic chip. As it continues to move downwards, it crushes the liquid sac, allowing the liquid inside to flow into the reaction chamber containing the sample. Subsequently, the crushing motor 51 rotates in the opposite direction to reset the crushing head 52. Then, the displacement module 4 drives the crushing head 52 to move to another liquid sac, so that liquid sacs containing different liquids can be injected into different reaction chambers at different times, thereby realizing automatic processes such as sample lysis, elution, or cleaning. In addition, a turntable with ball bearings can be installed on the base plate 1 below the chip stage 3. By raising the turntable to the bottom of the reaction chamber, the ball bearings squeeze the bottom of the reaction chamber, and the turntable rotates to push the ball bearings against the sample and liquid in the reaction chamber, thereby promoting the breakdown of the cell membrane or cell wall of the sample, so that the nucleic acid can flow out better.

[0036] Combination Figure 2 , Figure 4The pipetting module 6 is mounted on the top left side of the upright plate 11 and is on the same horizontal line as the displacement module 4 to make the detection device structure compact. The pipetting module 6 includes a pipetting motor 61, a pipetting screw 62, and a pipetting head 63. The pipetting motor 61 is fixed to the upright plate 11. The pipetting screw 62 is rotatably connected to the output end of the pipetting motor 61. The length direction of the pipetting screw 62 is vertical. The pipetting head 63 is threaded to the pipetting screw 62 and is connected to a smooth rod in the same direction as the axis of the pipetting screw 62 to prevent the pipetting head 63 from deflecting when it moves. An air tube is connected to one side of the pipetting head 63, and the bottom of the pipetting head 63 is open and communicates with the air tube. After the sample reacts with the liquid for a specified time, the controller built into the detection device starts the pipetting motor 61 to rotate and moves the pipetting head 63 down to the confluence of the microfluidic chip channels. Then, the air pump built into the detection device is activated to extract the gas from the channels of the microfluidic chip. At this time, due to the pressure difference, the liquid can be drawn from the current chamber to another chamber, realizing automatic liquid transfer, so as to carry out different processes such as lysis, elution, cleaning or replication.

[0037] Combination Figure 2 , Figure 4 The pneumatic module 7 includes a pneumatic motor 71, a longitudinal screw 72, a pneumatic support 73, and air ports 74. The pneumatic motor 71 is fixedly connected to the top of the base plate 1. The longitudinal screw 72 is rotatably connected to the output end of the pneumatic motor 71. The pneumatic support 73 is a strip-shaped structure. One side of the middle of the pneumatic support 73 is threadedly connected to the longitudinal screw 72. Both ends of the pneumatic support 73 are inserted with smooth rods in the same length direction as the longitudinal screw 72. The smooth rods are fixedly connected to the upright plate 11 to limit the deflection of the pneumatic support 73. Several air ports 74 are fixedly connected to the pneumatic support 73 at intervals. Each air port 74 is connected to an air tube below it. The air tube is also connected to an air pump with a solenoid valve. By controlling the opening and closing of different solenoid valves, air can be supplied to different air tubes. Since the microfluidic chip has multiple chambers and parallel chambers, the flow channels of the parallel chambers all extend with different air channels and extend from the bottom of the microfluidic chip to form air valve ports. When liquid diversion is required, the controller starts the pneumatic motor 71 to move. At this time, the pneumatic support 73 moves upward so that multiple air ports 74 are aligned with multiple air valve ports. Then, according to the input data, different solenoid valves are opened at timed intervals. At this time, air enters at one end of the flow channel and air is drawn out through the pipette head 63 at the other end. Since the other air ports 74 are closed at the bifurcation end of the flow channel, there is no air flow, which in turn causes the liquid to flow only in one direction to the designated chamber, thus achieving precise automatic liquid transfer.

[0038] Combination Figure 2 , Figure 5The heating module 8 includes a heating moving motor 81, a heating moving screw 82, a heating moving connecting seat 83, and a heating stage 84. The heating moving motor 81 is fixedly connected to the top of the base plate 1. The heating moving screw 82 is rotatably connected to the output end of the heating moving motor 81. The heating moving connecting seat 83 is threadedly connected to the heating moving screw 82. A slide rail slider with the same axial direction as the heating moving screw 82 is connected to one side of the heating moving connecting seat 83 to prevent the heating moving connecting seat 83 from deflecting when moving. The heating stage 84 is slidably connected to the heating moving connecting seat 83 and moves up and down. The heating stage 84 is equipped with heating elements to control the temperature between 60℃ and 95℃. To ensure accurate detection, the microfluidic chip generally has multiple amplification chambers and corresponding quantitative chambers connected in parallel. This facilitates nucleic acid amplification for fluorescence detection and simultaneously forms multiple control groups to determine the accuracy of the detection. The movable heating stage 84 allows it to provide suitable temperatures for nucleic acid amplification in different amplification chambers, accelerating the amplification speed so that users can see the detection results faster.

[0039] Combination Figure 6 , Figure 7 The heating movable connecting seat 83 has several vertical channels, and a heating element 831 is installed at the bottom of each channel. A wire sleeve 834 is provided on one side of the heating movable connecting seat 83 to allow a cable to pass through the heating element 831 for heating. A memory spring 832 made of composite shape memory polymer material is fixed to the heating element 831. The memory spring 832 in different channels is made of different materials. One of them is made of polycaprolactone and polyethylene glycol in a mass ratio of 8:2, mixed with 20% short-cut carbon fiber, and the melting point is controlled to 60°C. At this temperature, the memory spring 832 is preferably 10cm long, and its length is 8cm when compressed at room temperature. When the heating element 831 in the channel is heated to 60°C, the memory spring 832 can extend.

[0040] The heating elements 831 in different channels have different heating temperatures, ranging from 60℃ to 95℃. The memory spring 832 in another channel can be made of polycaprolactone and polylactic acid in a mass ratio of 3:7, mixed with 20% chopped carbon fibers, and its melting point is controlled to 95℃. At this temperature, the memory spring 832 is preferably 10cm long, and its length can be compressed to 8cm at room temperature. When the heating element 831 in this channel is heated to 95℃, the memory spring 832 can elongate.

[0041] Combination Figure 6 , Figure 7A cylindrical sliding post 833 abuts against a memory spring 832. The top of the sliding post 833 extends out of the heating movable connecting seat 83 and is fixedly connected to the heating stage 84. The heating element 831 and the heating element on the heating stage 84 can be of the same or different models. The memory spring 832 contains carbon fiber material to enhance stress so that it can smoothly lift the heating stage 84 when extended. By setting the memory spring 832 to automatically retract at room temperature, the heating stage 84 is prevented from contacting the microfluidic chip. When the heating element 831 and the heating element are heated to the specified temperature at the same time, the memory spring 832 extends to lift the heating stage 84, allowing the heating stage 84 to heat the amplification chamber. However, if the actual temperature does not reach the preset temperature, the heating stage 84 cannot contact the amplification chamber, resulting in the failure of subsequent fluorescence detection. In this case, the heating element or the heating element can be determined to be faulty and needs to be disassembled and replaced. Compared to using an additional temperature sensor, which cannot be inserted into the amplification chamber to detect the actual temperature of nucleic acid and will occupy additional space, preventing the device from being further reduced in size, this solution can also heat the amplification chamber if the temperature is not up to standard. It can only effectively heat the amplification chamber when the temperature is up to standard. It can also automatically control the movement of the heating stage 84, achieving two goals at once.

[0042] Combination Figure 2 , Figure 5 The fluorescence module 9 includes a fluorescence moving motor 91, a fluorescence moving screw 92, a fluorescence moving connector 93, and a fluorescence sensor 94. The fluorescence moving motor 91 is fixedly connected to the upright plate 11 on the same side as the heating moving motor 81. The fluorescence moving screw 92 is rotatably connected to the output end of the fluorescence moving motor 91. The fluorescence moving connector 93 is threadedly connected to the fluorescence moving screw 92. The bottom of the fluorescence moving connector 93 is connected to a slide rail slider in the same direction as the axis of the fluorescence moving screw 92 to prevent the fluorescence moving connector 93 from deflecting when it moves. The fluorescence sensor 94 is fixedly connected to the fluorescence moving connector 93 to perform fluorescence detection on the quantitative cavities distributed along the axis of the fluorescence moving screw 92, and to display the detection results on the screen 15 and the printer 16.

[0043] In summary, this invention significantly reduces the size of the detection device by arranging the various modules in a staggered, left-right configuration according to the structural characteristics of the microfluidic chip, using smaller components such as motors, and eliminating the need for traditional distance and temperature sensors, making it convenient for home use. Furthermore, its fully automated operation is user-friendly for novice users in other industries, allowing them to obtain accurate detection results without requiring specialized skills, thus facilitating market promotion.

[0044] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A microfluidic nucleic acid detection device, characterized in that: Includes a base plate (1), a chip inlet / outlet module (2) mounted on one side above the base plate (1), a chip stage (3) mounted on the chip inlet / outlet module (2) and carrying a microfluidic chip with a liquid bladder, a displacement module (4) mounted on the chip stage (3), a crushing module (5) mounted on the displacement module (4) and squeezing the liquid in the liquid bladder into the microfluidic chip, a pipetting module (6) mounted on one side of the crushing module (5) and pumping the liquid flow in the microfluidic chip, a pneumatic module (7) mounted above the front end of the base plate (1) and controlling the opening and closing of the microfluidic chip flow channel, and a component mounted on the base plate (1). On the other side above, there is a heating module (8) that locally heats the microfluidic chip and performs self-temperature testing without using a temperature sensor, and a fluorescence module (9) for collecting fluorescence signals from the microfluidic chip; a turntable with balls is installed on the base plate (1) below the chip stage (3). By raising the turntable to the bottom of the reaction chamber, the balls press against the bottom of the reaction chamber, and the turntable rotates to push the balls against the sample and liquid in the reaction chamber; the pipetting module (6) is mounted on the top left side of the upright plate (11) and is on the same horizontal line as the displacement module (4). The pipetting module (6) includes a pipetting motor (61) and a pipetting screw (62000). 2) and a pipette head (63), a pipette motor (61) fixedly connected to the upright plate (11), a pipette screw (62) rotatably connected to the output end of the pipette motor (61), the length direction of the pipette screw (62) being vertical, a pipette head (63) threadedly connected to the pipette screw (62) and connected to a smooth rod in the same direction as the axis of the pipette screw (62) to prevent the pipette head (63) from deflecting when moving, a gas tube connected to one side of the pipette head (63) to extract the gas in the flow channel inside the microfluidic chip; the pneumatic module (7) includes a pneumatic motor (71), a longitudinal screw (72), and a pneumatic support ( 73) and air inlets (74), pneumatic motor (71) is fixed above the base plate (1), longitudinal screw (72) is rotatably connected to the output end of pneumatic motor (71), pneumatic bracket (73) is threadedly connected to longitudinal screw (72) in the middle, and pneumatic bracket (73) has smooth rods inserted at both ends with the same length direction as longitudinal screw (72). The smooth rods are fixed to the upright plate (11) to limit the deflection of pneumatic bracket (73). Several air inlets (74) are fixedly connected to pneumatic bracket (73) at intervals. Each air inlet (74) is connected to an air pipe below it. The flow of liquid is controlled by passing air into different air pipes.

2. The microfluidic nucleic acid detection device according to claim 1, characterized in that: The heating module (8) includes a heating moving motor (81), a heating moving screw (82), a heating moving connecting seat (83), and a heating platform (84). The heating moving motor (81) is fixed above the base plate (1). The heating moving screw (82) is rotatably connected to the output end of the heating moving motor (81). The heating moving connecting seat (83) is threadedly connected to the heating moving screw (82). A slide rail slider with the same axial direction as the heating moving screw (82) is connected to one side of the heating moving connecting seat (83) to prevent the heating moving connecting seat (83) from deflecting when it moves. The heating platform (84) is slidably connected to the heating moving connecting seat (83) and moves up and down. The heating platform (84) is provided with heating elements to control the temperature between 60℃ and 95℃.

3. The microfluidic nucleic acid detection device according to claim 2, characterized in that: The heating movable connecting seat (83) has several vertical channels. A heating plate (831) is provided at the bottom of the channel. A memory spring (832) made of composite shape memory polymer material is fixedly connected to the heating plate (831). A cylindrical sliding column (833) is abutted on the memory spring (832). The top of the sliding column (833) extends out of the heating movable connecting seat (83) and is fixedly connected to the heating table (84).

4. A microfluidic nucleic acid detection device according to claim 3, characterized in that: The heating elements (831) in different channels have different heating temperatures, ranging from 60℃ to 95℃; the memory springs (832) in different channels all contain carbon fiber material to enhance stress, and the memory springs (832) elongate by 2cm when heated to 60℃.

5. A microfluidic nucleic acid detection device according to claim 1, characterized in that: The base plate (1) has square hollowed-out upright plates (11) fixedly connected to the left and right sides respectively. The chip platform (3) is a plate-shaped structure with several square holes or slots. The left and right sides of the chip platform (3) are slidably connected to the two upright plates (11) through slide rail sliders.

6. A microfluidic nucleic acid detection device according to claim 5, characterized in that: The chip in / out module (2) includes an in / out motor (21), an in / out screw (22), and a connecting frame (23). The in / out motor (21) is fixedly connected to the right upright plate (11). The in / out screw (22) is rotatably connected to the output end of the in / out motor (21). The connecting frame (23) is threadedly connected to the outside of the in / out screw (22). The connecting frame (23) is fixedly connected to the chip stage (3) to control the chip stage (3) to move back and forth in the horizontal direction.

7. A microfluidic nucleic acid detection device according to claim 6, characterized in that: The base plate (1) and the upright plate (11) are covered by a shell (13). The shell (13) covers the chip entry and exit module (2), chip stage (3), displacement module (4), crushing module (5), liquid pipetting module (6), pneumatic module (7), heating module (8) and fluorescence module (9) to form a black box inside the shell (13). The front end of the shell (13) is provided with an inlet and outlet (14) so ​​that the chip stage (3) extends out of the shell (13). The inlet and outlet (14) are hinged with doors to close the shell (13).

8. A microfluidic nucleic acid detection device according to claim 5, characterized in that: The displacement module (4) includes a displacement motor (41), a displacement screw (42), and a displacement platform (43). The displacement motor (41) is fixed to the top right side of the vertical plate (11). The displacement screw (42) is rotatably connected to the output end of the displacement motor (41). The axis of the displacement screw (42) is horizontal in the left and right direction. The displacement platform (43) is threaded to the outside of the displacement screw (42) and is connected to a slide rail slider with the same axis direction as the displacement screw (42) to prevent the displacement platform (43) from deflecting when it moves. The crushing module (5) is mounted on the displacement platform (43).

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