An anti-aging composition containing recombinant collagen and a preparation method thereof

Abstract

The application discloses an anti-aging composition containing recombinant collagen and a preparation method thereof and belongs to the technical field of anti-aging compositions. The preparation method comprises the following steps: preparing material A by taking salvia, uva-ursi leaves, pineapple pulp, salvia miltiorrhiza, caesalpinia and deionized water as raw materials; mixing deionized water and 1,3-butanediol, adding recombinant human type III collagen powder and scallop extract to obtain material B; mixing borneol extract, valerian extract, alocasia macrorrhiza extract, yizhi extract, isopropyl myristate, Tween-20, polyethylene glycol-400, material A and material B, and performing shear homogenization treatment to obtain material C; mixing deionized water and carbomer powder, adding carboxymethyl chitosan powder, adjusting the pH to neutral, adding material C and performing ultrasonic mixing to obtain the anti-aging composition; and the synergistic effect among the components is used to improve the anti-aging effect.

Description

Technical Field

[0001] This invention belongs to the field of anti-aging composition technology, specifically relating to an anti-aging composition containing recombinant collagen and its preparation method. Background Technology

[0002] Recombinant type III humanized collagen is produced using recombinant DNA technology. The human type III collagen gene fragment is genetically manipulated and introduced into host cells such as *E. coli* and yeast using vectors such as plasmids or viruses. This induces expression and translation into collagen or collagen-like peptides, followed by extraction and purification. It plays a crucial regulatory role in the body's skin damage and repair processes, possessing anti-inflammatory properties, maintaining skin elasticity, and enhancing cell activity.

[0003] Recombinant type III humanized collagen can inhibit the levels of matrix metalloproteinase-1 / interleukin-6 in tissues, reducing their damage to collagen and thus delaying skin aging. Furthermore, it can directly promote the expression of type I / type III collagen in aging tissues, driving the regeneration of both types, normalizing collagen fiber morphology, and restoring the supporting function of the extracellular matrix, thereby achieving skin rejuvenation.

[0004] Experiments have shown that recombinant type III humanized collagen, when used alone, does not have a significant anti-aging effect. However, when combined with specific ingredients, its anti-aging effect is significantly enhanced, exhibiting a synergistic effect. Nevertheless, there are many types of compound ingredients with anti-aging effects; therefore, how to select suitable compound ingredients to synergistically enhance the anti-aging effect with recombinant type III humanized collagen still requires further in-depth research. Summary of the Invention

[0005] The purpose of this invention is to provide an anti-aging composition containing recombinant collagen and its preparation method, which works synergistically with recombinant type III humanized collagen to enhance the anti-aging effect.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] A method for preparing an anti-aging composition containing recombinant collagen, the method comprising the following steps:

[0008] S1. Using sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water as raw materials, the mixture is heated and stirred in a water bath, filtered, the liquid phase is collected, and vacuum freeze-dried to obtain material A.

[0009] S2. Take deionized water and 1,3-butanediol, mix them, add recombinant type III humanized collagen powder and scallop extract to obtain material B;

[0010] S3. Take borneol extract, valerian extract, acorus extract, alpinia extract, isopropyl myristate, Tween-20, polyethylene glycol-400, material A, and material B, mix them, and shear and homogenize them to obtain material C.

[0011] S4. Take deionized water and carbomer powder, mix them, stir magnetically and let stand, add carboxymethyl chitosan powder, adjust the pH to neutral, add the material C, and mix ultrasonically to obtain the anti-aging composition.

[0012] As a preferred technical solution of the present invention, in step S1, the mass ratio of sage, bearberry leaf, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water is 15-25:14-18:35-55:3-5:6-8:100.

[0013] As a preferred technical solution of the present invention, the sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, and chicken blood vine can all be obtained through commercial sales or harvesting.

[0014] As a preferred embodiment of the present invention, the recombinant type III humanized collagen powder was purchased from Zhuhai Jibaikang Biotechnology Co., Ltd.

[0015] As a preferred embodiment of the present invention, the valerian extract is derived from the roots and rhizomes of Valeriana officinalis L., a plant of the Valerianaceae family.

[0016] As a preferred embodiment of the present invention, the Acorus gramineus extract is derived from the rhizome of Acorus gramineus Soland., a plant of the Araceae family.

[0017] As a preferred embodiment of the present invention, the Alpinia extract is derived from the dried, mature fruit of Alpinia oxyphylla, a plant belonging to the ginger family.

[0018] As a preferred embodiment of the present invention, isopropyl myristate, Tween-20, and polyethylene glycol-400 are all reagents that are well known and readily available to those skilled in the art.

[0019] As a preferred embodiment of the present invention, the visceral mass of fresh scallops is minced, homogenized with 1:1 (w / w) physiological saline, extracted at 4°C for 5 hours, centrifuged, and the supernatant is used for fractional precipitation with sodium sulfate. The precipitate with 75-85% saturation is dialyzed against Tris hydrochloric acid buffer (5 mmol / L, pH 7.5) until sulfate ions are removed. After concentration, the precipitate is subjected to chromatography on a DEAE-Sephadex A-50 column (18 mm × 80 mm) with a concentration of 0-0. The protein was eluted with a gradient of 0.1 mol / L sodium chloride solution at a flow rate of 0.5 mL / min and a detection wavelength of 280 nm. A 10 mL sample was collected per tube, and the protein peaks were combined. After concentration, the sample was dialyzed against a 5 mmol / L Tris hydrochloric acid buffer solution (pH 7.5) until chloride ions were eliminated. The protein was then lyophilized to obtain a lyophilized protein product. The lyophilized protein product was dissolved in deionized water and enzymatically hydrolyzed using a marine low-temperature alkaline protease. The hydrolysate was heated in a boiling water bath for 10 min and centrifuged. The liquid phase was then passed through a Sephadex G-25 column (16 mm × 600 mm) and eluted with deionized water at a flow rate of 2 mL / min and a detection wavelength of 280 nm. A 10 mL sample was collected per tube, and the protein peaks were combined. After concentration, the sample was lyophilized and ground to obtain the *Scallop scallop* extract.

[0020] As a preferred technical solution of the present invention, in step S1, the temperature of the vacuum freeze drying is -80 to -50°C, the vacuum degree is 0.1-5Pa, and the time is 20-30h.

[0021] As a preferred embodiment of the present invention, in step S2, the mass ratio of deionized water, 1,3-butanediol, recombinant type III humanized collagen powder, and scallop extract is 81.68-87.82:12-18:0.08-0.12:0.1-0.2.

[0022] As a preferred embodiment of the present invention, in step S3, the mass ratio of material A, material B, borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400 is 78-86:8.4-10.2:1.2-2.4:0.6-0.8:0.4-0.5:1-2:3-5:10-15:2-4.

[0023] As a preferred technical solution of the present invention, in step S4, the mass ratio of deionized water, carbomer powder, carboxymethyl chitosan powder, and material C is 25-35:6-8:1-3:18-32.

[0024] An anti-aging composition containing recombinant collagen was prepared using the above-described preparation method.

[0025] The beneficial effects of this invention are:

[0026] This invention utilizes recombinant type III humanized collagen, which is highly homologous to the skin's own collagen structure, allowing it to directly penetrate the dermis and replenish collagen fibers lost due to aging. It promotes fibroblast proliferation and migration, accelerates collagen synthesis, enhances skin elasticity, and reduces wrinkle depth. The antioxidant peptides in the scallop extract reduce oxidative stress levels, regulate the activity of superoxide dismutase (SOD) and catalase (CAT), and enhance the skin's own antioxidant defense system. Oxidative stress causes cross-linking and breakage of collagen fibers; antioxidant peptides reduce collagen oxidative modification by scavenging free radicals, prolonging the half-life of replenished collagen. The recombinant type III humanized collagen promotes the delivery of antioxidant peptides, enhancing the skin's antioxidant effect. Together, they activate skin repair signaling pathways, promote the expression of type I / type III collagen in aging tissues, and maintain skin elasticity, fundamentally improving the aging state of the skin.

[0027] Furthermore, by adding anti-aging ingredients such as sage, bearberry leaves, pineapple pulp, salvia miltiorrhiza, and chicken blood vine, the sodium tanshinone IIA sulfonate and salvianolic acid B in salvia miltiorrhiza, and the spatholobin in chicken blood vine improve microcirculation in the body, which can activate the TGF-β / Smad pathway, directly stimulate fibroblasts to express type III collagen genes, and promote the coordinated synthesis of type I and type III collagen; the arbutin in bearberry leaves can reduce glycation damage to collagen and maintain its elasticity; the rosmarinic acid in sage and the salvianolic acid B in salvia miltiorrhiza are powerful antioxidants that can scavenge superoxide anions and hydroxyl groups. It reduces free radicals and the oxidative breakdown of type III collagen by reactive oxygen species; the bromelain in pineapple not only gently metabolizes aged keratin, but its antioxidant peptides also enhance the activity level of glutathione in cells, improving the skin's own antioxidant capacity; recombinant type III humanized collagen directly replenishes skin structural proteins, and the introduction of sage, bearberry leaf, pineapple, salvia miltiorrhiza, and chicken blood vine promotes collagen synthesis, inhibits degradation, has antioxidant and anti-inflammatory effects, improves internal circulation and promotes absorption through synergistic effects, thus synergistically improving anti-aging effects, reducing wrinkle depth, and improving skin elasticity;

[0028] Furthermore, borneol extract gently stimulates the stratum corneum, widening the intercellular spaces and reducing the penetration resistance of anti-aging ingredients; valerian extract downregulates the expression of tight junction-related proteins, weakening the intercellular connections and making it easier for anti-aging ingredients to diffuse into the skin and be absorbed; acorus tatarinowii extract and alpinia oxyphylla extract improve skin circulation, preventing the accumulation of anti-aging ingredients locally and promoting their diffusion to fibroblasts, thus promoting the active uptake of anti-aging ingredients by cells; through the synergistic effect of these four ingredients, anti-aging ingredients can more effectively target collagen synthesis, anti-oxidation, and other targets in the dermis, enhancing the anti-aging effect.

[0029] Furthermore, by configuring a gel phase and physically blending it with anti-aging ingredients, the anti-aging effect can be reduced due to volatilization and oxidation of the anti-aging ingredients during absorption by the skin epidermis, thus maintaining the stability and uniformity of the system. Detailed Implementation

[0030] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0031] Example 1

[0032] A method for preparing an anti-aging composition containing recombinant collagen includes the following steps:

[0033] S1. Take sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water, mix them, shear and grind them, heat and stir in an 80℃ water bath for 20 min, filter, take the liquid phase, and freeze dry in vacuum at -80℃ and 0.1 Pa for 20 h to obtain material A; the mass ratio of sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water is 15:14:35:3:6:100;

[0034] S2. Take deionized water and 1,3-butanediol, mix them, stir for 4 min, add recombinant type III humanized collagen powder and scallop extract, stir for 15 min to obtain material B; the mass ratio of deionized water, 1,3-butanediol, recombinant type III humanized collagen powder and scallop extract is 81.68:12:0.08:0.1;

[0035] S3. Take borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400, mix them, stir for 10 min, add material A and material B, and shear homogenize at 8000 r / min for 3 min to obtain material C; the mass ratio of material A, material B, borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400 is 78:8.4:1.2:0.6:0.4:1:3:10:2;

[0036] S4. Take deionized water and carbomer powder, mix them, stir magnetically for 30 minutes, let stand for 6 hours, add carboxymethyl chitosan powder, continue stirring for 20 minutes, add triethanolamine to adjust the pH to neutral, add the material C, and ultrasonically stir for 25 minutes to obtain the anti-aging composition; the mass ratio of deionized water, carbomer powder, carboxymethyl chitosan powder and material C is 25:6:1:18.

[0037] Example 2

[0038] A method for preparing an anti-aging composition containing recombinant collagen includes the following steps:

[0039] S1. Take sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water, mix them, cut and grind them, heat and stir in an 85℃ water bath for 25 min, filter, take the liquid phase, and freeze dry it under vacuum at -80℃ and a vacuum degree of 0.1 Pa for 25 h to obtain material A; the mass ratio of sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water is 20:16:45:4:7:100;

[0040] S2. Take deionized water and 1,3-butanediol, mix them, stir for 5 min, add recombinant type III humanized collagen powder and scallop extract, stir for 20 min to obtain material B; the mass ratio of deionized water, 1,3-butanediol, recombinant type III humanized collagen powder and scallop extract is 84.75:15:0.1:0.15;

[0041] S3. Take borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400, mix them, stir for 15 min, add material A and material B, and shear homogenize at 10000 r / min for 5 min to obtain material C; the mass ratio of material A, material B, borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400 is 82:9.5:1.8:0.7:0.45:1.5:4:12:3;

[0042] S4. Take deionized water and carbomer powder, mix them, stir magnetically for 35 minutes, let stand for 7 hours, add carboxymethyl chitosan powder, continue stirring for 25 minutes, add triethanolamine to adjust the pH to neutral, add the material C, and ultrasonically stir for 30 minutes to obtain the anti-aging composition; the mass ratio of deionized water, carbomer powder, carboxymethyl chitosan powder and material C is 30:7:2:25.

[0043] Example 3

[0044] A method for preparing an anti-aging composition containing recombinant collagen includes the following steps:

[0045] S1. Take sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water, mix them, shear and grind them, heat and stir in a 90℃ water bath for 30 min, filter, take the liquid phase, and freeze dry it in vacuum at -80℃ and a vacuum degree of 5 Pa for 30 h to obtain material A; the mass ratio of sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water is 25:18:55:5:8:100;

[0046] S2. Take deionized water and 1,3-butanediol, mix them, stir for 6 min, add recombinant type III humanized collagen powder and scallop extract, stir for 25 min to obtain material B; the mass ratio of deionized water, 1,3-butanediol, recombinant type III humanized collagen powder and scallop extract is 87.82:18:0.12:0.2;

[0047] S3. Take borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400, mix them, stir for 20 minutes, add material A and material B, and shear homogenize at 12000 r / min for 4 minutes to obtain material C; the mass ratio of material A, material B, borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400 is 86:10.2:2.4:0.8:0.5:2:5:15:4;

[0048] S4. Take deionized water and carbomer powder, mix them, stir magnetically for 40 minutes, let stand for 8 hours, add carboxymethyl chitosan powder, continue stirring for 30 minutes, add triethanolamine to adjust the pH to neutral, add the material C, and ultrasonically stir for 35 minutes to obtain the anti-aging composition; the mass ratio of deionized water, carbomer powder, carboxymethyl chitosan powder and material C is 35:8:3:32.

[0049] Comparative Example 1

[0050] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain sage. The amount of sage is reduced and allocated to bearberry leaves, pineapple flesh, salvia miltiorrhiza, and chicken blood vine in proportion to the number of parts. The other components and their amounts remain unchanged.

[0051] Comparative Example 2

[0052] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain bearberry leaves. The reduced amount of bearberry leaves is allocated to sage, pineapple pulp, salvia miltiorrhiza, and chicken blood vine in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0053] Comparative Example 3

[0054] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain pineapple pulp. The reduced amount is allocated to sage, bearberry leaves, salvia miltiorrhiza, and chicken blood vine in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0055] Comparative Example 4

[0056] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain Salvia miltiorrhiza. The reduced amount of Salvia miltiorrhiza is allocated to Salvia miltiorrhiza, Ardisia crenata leaves, pineapple flesh, and Spatholobus suberectus in proportion to the proportions of the other components. The other components and their amounts remain unchanged.

[0057] Comparative Example 5

[0058] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain chicken blood vine. The reduced amount of chicken blood vine is allocated to sage, bearberry leaves, pineapple flesh, and salvia miltiorrhiza in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0059] Comparative Example 6

[0060] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain recombinant type III humanized collagen powder. The amount of recombinant type III collagen powder is reduced and allocated to the scallop extract in parts. The remaining components and amounts remain unchanged.

[0061] Comparative Example 7

[0062] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain scallop extract. The reduced amount of scallop extract is allocated to the recombinant type III humanized collagen powder in parts by proportion, while the remaining components and amounts remain unchanged.

[0063] Comparative Example 8

[0064] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain borneol extract. The reduced amount is allocated to valerian extract, acorus extract, and alpinia extract in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0065] Comparative Example 9

[0066] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain valerian extract. The reduced amount of valerian extract is allocated to borneol extract, acorus extract, and alpinia extract in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0067] Comparative Example 10

[0068] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain Acorus tatarinowii extract. The reduced amount is allocated to borneol extract, valerian extract, and Alpinia oxyphylla extract in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0069] Comparative Example 11

[0070] This comparative example provides an anti-aging composition. The only difference between this composition and Example 2 is that it does not contain Alpinia oxyphylla extract. The reduced amount of Alpinia oxyphylla extract is allocated to borneol extract, valerian extract, and acorus calamus extract in proportion to the number of parts. The remaining components and their amounts remain unchanged.

[0071] Comparative Example 12

[0072] A method for preparing an anti-aging composition containing recombinant collagen includes the following steps:

[0073] S1. Take sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water, mix them, cut and grind them, heat and stir in an 85℃ water bath for 25 min, filter, take the liquid phase, and freeze dry it under vacuum at -80℃ and a vacuum degree of 0.1 Pa for 25 h to obtain material A; the mass ratio of sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water is 20:16:45:4:7:100;

[0074] S2. Take deionized water and 1,3-butanediol, mix them, stir for 5 min, add recombinant type III humanized collagen powder and scallop extract, stir for 20 min to obtain material B; the mass ratio of deionized water, 1,3-butanediol, recombinant type III humanized collagen powder and scallop extract is 84.75:15:0.1:0.15;

[0075] S3. Take borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400, mix them, stir for 15 minutes, add material A and material B, and shear homogenize at 10000 r / min for 5 minutes to obtain the anti-aging composition; the mass ratio of material A, material B, borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400 is 82:9.5:1.8:0.7:0.45:1.5:4:12:3.

[0076] Performance testing

[0077] Test Example 1

[0078] One hundred and sixty Swiss mice, 2 months old, weighing 18-22g, half male and half female, were randomly divided into 16 groups of 10 mice each. An aging model was constructed by subcutaneous injection of D-galactose 1g / kg / d for 45 consecutive days. The mice showed signs of darkening of fur, hair loss, and slow movement. As time went on, the mice gradually began to show obvious signs of aging such as cold intolerance, huddling together, and decreased appetite, which indicated that the model was successfully constructed. Fifteen of the aging mouse groups were designated as the experimental group and one group was designated as the control group.

[0079] Ten Swiss mice, 2 months old, weighing 18-22g, half male and half female, were used to construct a normal group without any treatment.

[0080] Before the experiment, the back (both sides of the spine, under the scapula) area of ​​the aging mice in the experimental group was shaved with electric scissors (each area was 2cm × 2cm). The next day, the anti-aging composition prepared in Examples 1-3 and Comparative Examples 1-12 was applied to the shaved area at a dose of 0.5g / kg, twice a day for 60 consecutive days. The back (both sides of the spine, under the scapula) area of ​​the aging mice in the control group was shaved with electric scissors (each area was 2cm × 2cm). The next day, an equal dose of physiological saline was applied. The back (both sides of the spine, under the scapula) area of ​​the untreated mice in the normal group was shaved with electric scissors (each area was 2cm × 2cm). The next day, an equal dose of physiological saline was applied.

[0081] During the experiment, all groups of mice had free access to food and water.

[0082] The content of antioxidants in the skin tissue homogenates of mice in each group was detected, and the test results are shown in Table 1 below.

[0083] Table 1

[0084]

[0085] As shown in Table 1, the anti-aging compositions prepared in Examples 1-3 of this application have good anti-aging effects; the lack of synergistic effects between sage, bearberry leaf, pineapple flesh, salvia miltiorrhiza, and chicken blood vine in Comparative Examples 1-5 resulted in a reduced anti-aging effect; the lack of synergistic effects between recombinant type III humanized collagen powder and scallop extract in Comparative Examples 6-7 resulted in a reduced anti-aging effect; the lack of synergistic effects between borneol extract, valerian extract, acorus calamus extract, and alpinia oxyphylla extract in Comparative Examples 8-11 resulted in a reduced anti-aging effect; and the absence of gel loading of anti-aging components in Comparative Example 12 resulted in oxidation loss and volatilization of the anti-aging components, leading to a reduced anti-aging effect.

[0086] Test Example 2

[0087] Seventy-five volunteers (male to female ratio of 17:58, aged 44-60 years) with obvious facial wrinkles, dull skin, and loose skin were randomly selected and randomly divided into 15 groups of 5 people each. Each group applied 1g of one of the anti-aging compositions from Examples 1-3 and Comparative Examples 1-12 once a day for 28 consecutive days. During this period, no other skin care products or products with similar effects were used. Changes in facial skin indicators were tested, and the test results are shown in Table 2 below.

[0088] Table 2

[0089]

[0090] As shown in Table 2, the anti-aging compositions prepared in Examples 1-3 of this application have good wrinkle-reducing and skin elasticity-restoring effects. Comparative Examples 1-5 lack the synergistic effect between sage, bearberry leaf, pineapple pulp, salvia miltiorrhiza, and chicken blood vine, resulting in reduced wrinkle-reducing and skin elasticity-restoring effects. Comparative Examples 6-7 lack the synergistic effect between recombinant type III humanized collagen powder and scallop extract, resulting in reduced wrinkle-reducing and skin elasticity-restoring effects. Comparative Examples 8-11 lack the synergistic effect between borneol extract, valerian extract, acorus tatarinowii extract, and alpinia oxyphylla extract, resulting in reduced wrinkle-reducing and skin elasticity-restoring effects. Comparative Example 12 did not use gel-loaded anti-aging ingredients, leading to oxidation loss and volatilization of the anti-aging ingredients, thus reducing the wrinkle-reducing and skin elasticity-restoring effects.

[0091] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A method for preparing an anti-aging composition containing recombinant collagen, characterized in that, The method for preparing the anti-aging composition includes the following steps: S1. Using sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine and deionized water as raw materials, the mixture is heated and stirred in a water bath, filtered, the liquid phase is collected, and vacuum freeze-dried to obtain material A. S2. Take deionized water and 1,3-butanediol, mix them, add recombinant type III humanized collagen powder and scallop extract to obtain material B; S3. Take borneol extract, valerian extract, acorus extract, alpinia extract, isopropyl myristate, Tween-20, polyethylene glycol-400, material A, and material B, mix them, and shear and homogenize them to obtain material C. S4. Take deionized water and carbomer powder, mix them, stir magnetically and let stand, add carboxymethyl chitosan powder, adjust the pH to neutral, add the material C, and mix ultrasonically to obtain the anti-aging composition.

2. The method for preparing an anti-aging composition containing recombinant collagen according to claim 1, characterized in that, In step S1, the mass ratio of sage, bearberry leaves, pineapple flesh, salvia miltiorrhiza, chicken blood vine, and deionized water is 15-25:14-18:35-55:3-5:6-8:

100.

3. The method for preparing an anti-aging composition containing recombinant collagen according to claim 1, characterized in that, In step S1, the vacuum freeze-drying temperature is -80 to -50°C, the vacuum degree is 0.1-5Pa, and the time is 20-30h.

4. The method for preparing an anti-aging composition containing recombinant collagen according to claim 1, characterized in that, In step S2, the mass ratio of deionized water, 1,3-butanediol, recombinant type III humanized collagen powder, and scallop extract is 81.68-87.82:12-18:0.08-0.12:0.1-0.

2.

5. The method for preparing an anti-aging composition containing recombinant collagen according to claim 1, characterized in that, In step S3, the mass ratio of material A, material B, borneol extract, valerian extract, acorus tatarinowii extract, alpinia oxyphylla extract, isopropyl myristate, Tween-20, and polyethylene glycol-400 is 78-86:8.4-10.2:1.2-2.4:0.6-0.8:0.4-0.5:1-2:3-5:10-15:2-4.

6. The method for preparing an anti-aging composition containing recombinant collagen according to claim 1, characterized in that, In step S4, the mass ratio of deionized water, carbomer powder, carboxymethyl chitosan powder, and material C is 25-35:6-8:1-3:18-32.

7. An anti-aging composition containing recombinant collagen prepared by the preparation method according to any one of claims 1-6.

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