Anti-aging soft capsule and preparation method thereof

Abstract

The invention discloses an anti-aging soft capsule and a preparation method thereof. The anti-aging soft capsule is prepared from the following raw materials: 40 to 50 parts of anthocyanin extract, 15 to 20 parts of pyrroloquinoline quinone, 80 to 85 parts of soybean phospholipid, 820 to 830 parts of fish oil, 20 to 30 parts of beewax, 100 to 120 parts of gelatin, 50 to 60 parts of glycerol and 100 to 120 parts of water. Wherein the pyrroloquinoline quinone and the soybean phospholipid are subjected to nanocrystallization to form the nano-liposome. According to the application, PQQ and soybean phospholipid are subjected to nanocrystallization to prepare the specific nano-liposome, so that the absorption efficiency of the nano-liposome in intestinal tracts is effectively improved, and various functional components such as fish oil and anthocyanin are matched, so that the DPPH free radical scavenging rate and the SOD activity can be improved, and aging is delayed from multiple dimensions such as oxidation resistance, inflammation resistance, mitochondrial repair and membrane system steady state.

Description

Technical Field

[0001] This application relates to an anti-aging soft capsule and its preparation method, belonging to the field of health care preparation technology. Background Technology

[0002] With the improvement of living standards, people are paying increasing attention to health and aging issues. The aging process is accompanied by a gradual decline in bodily functions, and the excessive production of free radicals is one of the important factors leading to aging. Free radicals attack intracellular biomolecules, such as lipids, proteins, and DNA, triggering oxidative stress damage, which in turn accelerates cellular aging and the aging process of the body. Therefore, developing nutritional intervention products that can effectively scavenge free radicals and enhance antioxidant capacity is of great significance.

[0003] There are many anti-aging health products on the market, but their functions and effects are not ideal, mainly due to the following problems: 1. Low absorption efficiency of antioxidant ingredients: For example, Chinese patent application (CN119868441A) uses proanthocyanidins and Ganoderma lucidum polysaccharides in combination, and also adds (pyrroloquinoline quinone) PQQ to prevent or improve neurological diseases in the elderly. However, in reality, PQQ has a low oral intestinal absorption rate and its effect is limited. 2. Single mechanism of action: Existing products often only target anti-oxidation or other single functions, simply physically mixing the ingredients, which not only results in poor absorption but also makes it difficult to meet the multi-level anti-aging needs.

[0004] Therefore, it is of great significance to develop an anti-aging product that has high absorption efficiency, multi-dimensional synergistic anti-aging effects, and is safe and effective. Summary of the Invention

[0005] To address the aforementioned issues, an anti-aging soft capsule and its preparation method are provided. By nano-sizing PQQ with soybean phospholipids to prepare specific nanoliposomes, the absorption efficiency in the intestine is effectively improved. Combined with various functional ingredients such as fish oil and anthocyanins, it effectively enhances DPPH free radical scavenging rate and SOD activity, thereby delaying aging from multiple dimensions such as anti-oxidation, anti-inflammation, mitochondrial repair, and membrane system homeostasis.

[0006] According to one aspect of this application, an anti-aging soft capsule is provided, comprising the following raw materials: 40-50 parts of anthocyanin extract, 15-20 parts of pyrrolquinone, 80-85 parts of soybean lecithin, 820-830 parts of fish oil, 20-30 parts of beeswax, 100-120 parts of gelatin, 50-60 parts of glycerin, and 100-120 parts of water; wherein the pyrrolquinone and soybean lecithin are nano-sized to form nanoliposomes.

[0007] Optionally, the nanoliposomes have a particle size of 20-200 nm, an encapsulation efficiency of not less than 85%, and a drug loading of not less than 15%.

[0008] Specifically, by nanoforming pyrroloquinoline quinone (PQQ) with soybean phospholipids to form nanoliposomes of a specific particle size, these nanoliposomes can be efficiently taken up by intestinal epithelial cells, thereby significantly improving the transmembrane transport rate of PQQ and thus significantly enhancing the absorption efficiency of PQQ in the gastrointestinal tract. Simultaneously, the nanoliposomes, under the action of gastrointestinal enzymes or phospholipases at the intestinal mucosa, can gradually release PQQ, achieving sustained-release and controlled-release. Furthermore, the liposome membrane itself can fuse with the cell membrane, directly "inserting" PQQ into the cell membrane or intracellular vesicles, achieving a rapid initiation mechanism. This dual release mode (continuous release + membrane fusion) greatly increases the peak and average concentrations of PQQ in the submucosa of the intestinal tract and in the blood, further promoting mitochondrial targeted delivery.

[0009] Furthermore, PQQ, as an excellent non-covalent cofactor, participates in the electron transfer cycle in the mitochondrial dehydrogenase system and can directly scavenge various reactive oxygen species such as hydroxyl radicals. Nanoparticle delivery increases its intracellular deposition level, effectively improving the DPPH radical scavenging rate. PQQ can also promote mitochondrial proliferation and membrane potential recovery. Nanoparticle delivery allows PQQ to reach the threshold concentration in cells in a short time, significantly increasing mitochondrial ATP production and effectively slowing down cellular aging caused by mitochondrial functional decline. Further, the nanoparticles contain soybean phospholipids, which can replenish the phosphatidylcholine component of cell membranes and repair oxidatively damaged membrane structures. Simultaneously, phospholipids themselves can regulate the secretion of inflammatory factors, working in conjunction with the anti-inflammatory effects of EPA / DHA in fish oil, ultimately achieving a three-dimensional, multi-dimensional synergistic anti-aging effect of "antioxidant-anti-inflammatory-membrane repair".

[0010] Optionally, the weight ratio of the pyrroloquinoline quinone to the soybean lecithin is 1:(4-5).

[0011] Specifically, by limiting the weight ratio of pyrroloquinoline quinone to soybean phospholipids, the stability of the liposome membrane structure is improved. The hydrophobic tail of soybean phospholipids and the quinone ring of PQQ are tightly bound together through π-π stacking to form a stable liposome bilayer structure. The ratio of phospholipids to PQQ determines the membrane thickness and fluidity of the bilayer liposomes. If the ratio is too low, the phospholipids are insufficient to form a continuous and stable bilayer membrane, which easily leads to membrane rupture and leakage of contents. If the ratio is too high, the excess phospholipids result in an excessively thick membrane, increasing shear instability during the preparation process and making the particle size larger. Within this range, phospholipid molecules can form a uniform bilayer membrane with moderate fluidity, ensuring the interfacial tension balance between the inner and outer phases of the membrane while also taking into account the controllability of the preparation process.

[0012] On the other hand, liposomes prepared at this ratio have a stable particle size within a defined range, enabling them to be rapidly taken up into intestinal epithelial cells, reducing the risk of aggregation in intestinal fluid or retention by mucus. Furthermore, the membrane structure is neither easily degraded prematurely by enzymes nor completely passivated in acidic gastric juice.

[0013] Optionally, the method for preparing the nanoliposomes includes the following steps: a. Dissolve soybean lecithin in chloroform and pyrroloquinoline quinone in phosphate buffer; b. Under magnetic stirring, pyrroloquinoline quinone buffer solution was added dropwise to soybean lecithin chloroform solution, and stirring was continued to obtain colostrum; c. Transfer the colostrum to a rotary evaporator and remove chloroform by rotary evaporation, stirring continuously until a translucent nanoliposome solution is obtained; d. The nanoliposome solution is repeatedly ultrafiltered through an ultrafiltration membrane to remove unencapsulated material until the particle size is 20-200 nm, thus obtaining the nanoliposomes.

[0014] Specifically, in step c, the stirring time is 40 min, in step d, an ultrafiltration membrane of 0.45 μm is used for ultrafiltration, the pH of the phosphate buffer solution is 7.4, the ratio of chloroform to soybean lecithin is 3:1, and pyrroloquinoline quinone is dissolved in phosphate buffer at a concentration of 8 mg / mL.

[0015] In this application, PQQ molecules fully expand in the aqueous phase, while soybean phospholipid molecules are in a freely diffused state in the organic solvent. During subsequent dropwise addition, the two molecules can directly meet at the interface and spontaneously form a microemulsion precursor, avoiding the encapsulation loss caused by the traditional method of coarse dispersion followed by fine dispersion. Constant-rate dropwise addition and continuous stirring ensure that each microdroplet of the aqueous phase is rapidly encapsulated around the phospholipid membrane, forming nanoscale proemulsion microdroplets with a narrow particle size distribution, laying the foundation for subsequent nano-sizing. Chloroform is gradually evaporated under medium-low temperature and reduced pressure conditions to prevent the phospholipid bilayer from being destroyed by high temperature or severe shear, maintaining the integrity of the liposome vesicle structure. Repeated filtration using a 0.45µm ultrafiltration membrane removes unencapsulated PQQ, free phospholipid fragments, and large particulate impurities. Ultrafiltration not only efficiently retains free small molecules and large particles but also slightly modulates the surface charge and morphology of vesicles through shearing, resulting in more uniform liposome dispersion and preventing aggregation and sedimentation.

[0016] Optionally, in step b, the stirring rate is 500-800 rpm; the dropping rate is 0.5-1 mL / s; and the stirring time is 30-40 min.

[0017] Optionally, the rotary evaporation conditions in step c are: temperature 40-45℃, vacuum degree 0.08-0.10MPa, and rotation speed 50-100rpm.

[0018] Optionally, the fish oil contains at least 60% ω-3 polyunsaturated fatty acids.

[0019] Preferably, the anthocyanin extract contains no less than 95% proanthocyanidins.

[0020] According to one aspect of this application, a method for preparing the above-mentioned anti-aging soft capsules is provided, comprising the following steps: (1) Weigh fish oil and beeswax, heat to dissolve, and cool to room temperature to form an oil-wax liquid; (2) After filtering and heat preservation, gelatin, glycerin and hydrated gelatin are added; (3) Mix the oil and wax liquid obtained in step (1) with the glue liquid obtained in step (2), anthocyanin extract, and PQQ-soybean phospholipid nanoliposomes evenly, let stand, and then press into pellets to obtain the anti-aging soft capsules.

[0021] Optionally, the heating temperature in step (1) is 65℃-75℃, and the sol temperature in step (2) is 60℃-80℃ after the gel is dissolved and filtered through a 100-mesh filter.

[0022] Optionally, in step (3), the anthocyanin extract and PQQ-soybean phospholipid nanoliposomes are passed through an 80-mesh sieve and then mixed with the oil wax liquid and the glue liquid.

[0023] The beneficial effects of this application include, but are not limited to: 1. The anti-aging soft capsule of this application uses PQQ and soybean phospholipids to prepare specific nanoliposomes, which can be efficiently endocytosed and transported by epithelial cells in the intestine. The prepared nanoliposomes can enable PQQ to rapidly reach an effective peak concentration in a short period of time, effectively improve mitochondrial membrane potential, and enhance mitochondrial energy metabolism efficiency.

[0024] 2. The anti-aging soft capsules of this application incorporate multiple auxiliary ingredients: utilizing the potent free radical scavenging ability of anthocyanin extract, it can directly capture ·OH and O2. - • Reactive oxygen species; the high content of ω-3 in fish oil further inhibits the inflammatory cascade by regulating cell membrane fluidity and the secretion of inflammatory mediators; soybean phospholipids not only constitute liposome carriers but also replenish phosphatidylcholine in cell membranes, repairing oxidatively damaged membrane structures. These four components are delivered simultaneously within the nanoliposome carrier, exhibiting a multi-dimensional synergistic effect across the entire chain of "antioxidant-anti-inflammatory-mitochondrial regulation-membrane repair," thus meeting multi-level anti-aging needs.

[0025] 3. The preparation method of the anti-aging soft capsules of this application can obtain uniform and controllable particle size nanoliposomes in four steps: phase separation (chloroform / aqueous phase) - constant rate dropwise addition of colostrum - low temperature vacuum rotary evaporation - ultrafiltration purification. Combined with the oil and wax pre-emulsification and gelatin / glycerol soft capsule shell integrated pelleting process, there is no need for high pressure homogenization or multi-stage ultrasound, which significantly reduces equipment investment and energy consumption. Moreover, the process parameters (dropping rate, temperature / vacuum, ultrafiltration pressure difference) can be monitored online to ensure batch-to-batch consistency and good product stability. Detailed Implementation

[0026] The present application is described in detail below with reference to the embodiments, but the present application is not limited to these embodiments.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of skill in the art. The reagents and raw materials used in this invention are readily available through conventional means, and unless otherwise specified, they shall be used in accordance with conventional methods in the art or as per the product instructions. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to the methods of this invention. The preferred embodiments and materials described in this patent are for illustrative purposes only.

[0028] The sources and types of raw materials used in this application are as follows: Anthocyanin extract: Hypericum St. John's Wort Extract (purity ≥95%), Shaanxi Huike Plant Development Co., Ltd.; Pyrroloquinoline quinone (PQQ): BioPQQ ® FL, Mitsubishi Gas Chemical Co., Ltd., Japan; Soybean lecithin: Phospholipon 90G (PC≥95%), Lipoid GmbH, Germany; Fish oil: EPAX 6000 TG, Aker BioMarine ASA, Norway; Beeswax: Apis mellifera Beeswax, Shandong Huakang Bee Industry Co., Ltd., China; Gelatin: Gelita Type B, Gelita Group, Germany; Glycerin: USP Pharmaceutical Grade (purity ≥99.5%), Dow Chemical Company, USA.

[0029] Example 1: Anti-aging soft capsule #1 Anti-aging soft capsule #1 is made from the following ingredients: 45 parts anthocyanin extract, 16 parts pyrroloquinoline quinone, 80 parts soybean lecithin, 825 parts fish oil, 25 parts beeswax, 110 parts gelatin, 55 parts glycerin, and 110 parts water. In this process, pyrroloquinoline quinone and soybean lecithin are nano-sized to form liposomes. The liposomes have a particle size of 100 nm, an encapsulation efficiency of 90%, and a drug loading of 20%. The weight ratio of pyrroloquinoline quinone to soybean lecithin is 1:5. The preparation method of the liposomes includes the following steps: a. Dissolve soybean lecithin in chloroform and pyrroloquinoline quinone in phosphate buffer; b. Under magnetic stirring, pyrroloquinoline quinone buffer solution was added dropwise to soybean lecithin chloroform solution, and stirring was continued to obtain colostrum; c. Transfer the colostrum to a rotary evaporator and remove chloroform by rotary evaporation, stirring continuously until a translucent nanoliposome solution is obtained; d. Repeatedly ultrafilter the nanoliposome solution through an ultrafiltration membrane to remove unencapsulated material until the particle size is 100 nm to obtain nanoliposomes.

[0030] In step b, the stirring rate was 600 rpm; the dropping rate was 0.8 mL / s; and the stirring time was 35 min. In step c, the rotary evaporation conditions were: temperature 42℃, vacuum degree 0.09 MPa, and rotation speed 80 rpm. The fish oil contained 80% ω-3 polyunsaturated fatty acids.

[0031] The preparation method of anti-aging soft capsule 1# includes the following steps: (1) Weigh fish oil and beeswax, heat to dissolve, and cool to room temperature to form an oil-wax liquid; (2) After filtering and heat preservation, gelatin, glycerin and hydrated gelatin are added; (3) Mix the oil and wax liquid obtained in step (1) with the glue liquid obtained in step (2), anthocyanin extract, and PQQ-soybean phospholipid nanoliposomes evenly, let stand, and then press into pellets to obtain anti-aging soft capsule 1#.

[0032] In step (1), the heating temperature is 70℃. In step (2), the gel is filtered through a 100-mesh sieve and the sol temperature is 70℃. In step (3), the anthocyanin extract and PQQ-soybean phospholipid nanoliposomes are sieved through an 80-mesh sieve and then mixed with the oil wax liquid and the gel liquid.

[0033] Example 2 Anti-aging soft capsule #2 Anti-aging soft capsule #2 is made from the following ingredients: 40 parts anthocyanin extract, 18 parts pyrroloquinoline quinone, 81 parts soybean lecithin, 820 parts fish oil, 20 parts beeswax, 100 parts gelatin, 50 parts glycerin, and 100 parts water. In this process, pyrroloquinoline quinone and soybean lecithin are nano-sized to form liposomes. The liposomes have a particle size of 20 nm, an encapsulation efficiency of 87%, and a drug loading of 18%. The weight ratio of pyrroloquinoline quinone to soybean lecithin is 1:4.5. The preparation method of the liposomes includes the following steps: a. Dissolve soybean lecithin in chloroform and pyrroloquinoline quinone in phosphate buffer; b. Under magnetic stirring, pyrroloquinoline quinone buffer solution was added dropwise to soybean lecithin chloroform solution, and stirring was continued to obtain colostrum; c. Transfer the colostrum to a rotary evaporator and remove chloroform by rotary evaporation, stirring continuously until a translucent nanoliposome solution is obtained; d. The nanoliposome solution is repeatedly ultrafiltered through an ultrafiltration membrane to remove unencapsulated material until the particle size is 20 nm, thus obtaining nanoliposomes.

[0034] In step b, the stirring rate was 500 rpm; the dropping rate was 0.5 L / s; and the stirring time was 30 min. In step c, the rotary evaporation conditions were: temperature 45℃, vacuum degree 0.10 MPa, and rotation speed 100 rpm. The fish oil contained 75% ω-3 polyunsaturated fatty acids.

[0035] The preparation method of anti-aging soft capsule #2 includes the following steps: (1) Weigh fish oil and beeswax, heat to dissolve, and cool to room temperature to form an oil-wax liquid; (2) After filtering and heat preservation, gelatin, glycerin and hydrated gelatin are added; (3) Mix the oil and wax liquid obtained in step (1) with the glue liquid obtained in step (2), anthocyanin extract, and PQQ-soybean phospholipid nanoliposomes evenly, let stand, and then press into pellets to obtain anti-aging soft capsule 2#.

[0036] In step (1), the heating temperature is 65℃. In step (2), the gel is filtered through a 100-mesh sieve and the sol temperature is 60℃. In step (3), the anthocyanin extract and PQQ-soybean phospholipid nanoliposomes are sieved through an 80-mesh sieve and then mixed with the oil wax liquid and the gel liquid.

[0037] Example 3 Anti-aging soft capsules #3 Anti-aging soft capsule #3 is made from the following ingredients: 50 parts anthocyanin extract, 20 parts pyrroloquinoline quinone, 80 parts soybean lecithin, 830 parts fish oil, 30 parts beeswax, 120 parts gelatin, 60 parts glycerin, and 120 parts water. In this process, pyrroloquinoline quinone and soybean lecithin are nano-sized to form liposomes. The liposomes have a particle size of 200 nm, an encapsulation efficiency of 85%, and a drug loading of 15%. The weight ratio of pyrroloquinoline quinone to soybean lecithin is 1:4. The preparation method of the liposomes includes the following steps: a. Dissolve soybean lecithin in chloroform and pyrroloquinoline quinone in phosphate buffer; b. Under magnetic stirring, pyrroloquinoline quinone buffer solution was added dropwise to soybean lecithin chloroform solution, and stirring was continued to obtain colostrum; c. Transfer the colostrum to a rotary evaporator and remove chloroform by rotary evaporation, stirring continuously until a translucent nanoliposome solution is obtained; d. Repeatedly ultrafilter the nanoliposome solution through an ultrafiltration membrane to remove unencapsulated material until the particle size is 20-200 nm to obtain nanoliposomes.

[0038] In step b, the stirring rate was 800 rpm; the dropping rate was 1 mL / s; and the stirring time was 40 min. In step c, the rotary evaporation conditions were: temperature 40℃, vacuum degree 0.08 MPa, and rotation speed 50 rpm. The fish oil contained 85% ω-3 polyunsaturated fatty acids.

[0039] The preparation method of anti-aging soft capsule #3 includes the following steps: (1) Weigh fish oil and beeswax, heat to dissolve, and cool to room temperature to form an oil-wax liquid; (2) After filtering and heat preservation, gelatin, glycerin and hydrated gelatin are added; (3) Mix the oil and wax liquid obtained in step (1) with the glue liquid obtained in step (2), anthocyanin extract, and PQQ-soybean phospholipid nanoliposomes evenly, let stand, and then press into pellets to obtain anti-aging soft capsule 3#.

[0040] In step (1), the heating temperature is 75℃. In step (2), the gel is filtered through a 100-mesh sieve and the sol temperature is 80℃. In step (3), the anthocyanin extract and PQQ-soybean phospholipid nanoliposomes are sieved through an 80-mesh sieve and then mixed with the oil wax liquid and the gel liquid.

[0041] Example 4 Anti-aging soft capsule #4 The difference between Example 4 and Example 1 is that the particle size of the nanoliposomes in Example 4 is 350 nm.

[0042] Example 5: Anti-aging soft capsule #5 The difference between Example 5 and Example 1 is that the weight ratio of pyrroloquinoline quinone to soybean lecithin in Example 5 is 1:7.

[0043] Comparative Example 1 vs. Anti-aging Soft Capsules #1 The difference between Comparative Example 1 and Example 1 is that soybean lecithin is not used in Comparative Example 1, but zein is used instead.

[0044] Comparative Example 2 vs. Anti-aging Soft Capsules 2# The difference between Comparative Example 2 and Example 1 is that no nanoliposomes were prepared in Comparative Example 2.

[0045] Comparative Example 3 vs. Anti-aging Soft Capsules #3 The difference between Comparative Example 3 and Example 1 is that no anthocyanin extract was used in Comparative Example 3.

[0046] Experimental Example The contents of the anti-aging soft capsules prepared in Examples 1-5 and Comparative Examples 1-3 were used to conduct experiments on DPPH free radical scavenging rate and mouse liver SOD activity.

[0047] Experimental methods: I. DPPH Free Radical Scavenging Rate Determination Experiment Reference standard: GB / T 5009.171-2003 "Determination of superoxide dismutase (SOD) activity in health food"; The experiment adopted a randomized block design, with 3 parallel samples set in each group of Examples 1-5 and Comparative Examples 1-3, and the data are expressed as mean ± standard deviation.

[0048] Experimental steps: Reagent preparation: DPPH solution: Accurately weigh 0.006 g DPPH (Sigma-Aldrich), dissolve in anhydrous ethanol and bring the volume to 100 mL, store protected from light. Ethanol solution: Analytical grade anhydrous ethanol (chromatographic grade).

[0049] 1. Sample preparation Take 100 mg of the sample to be tested (soft capsule contents), add 10 mL of anhydrous ethanol, extract by sonication for 30 min (power 300 W, frequency 40 kHz), centrifuge (10000 rpm, 10 min), and take the supernatant for later use.

[0050] 2. Measurement Method Blank group: 1 mL anhydrous ethanol + 3 mL DPPH solution; Control group: 1 mL sample solution + 3 mL anhydrous ethanol; Experimental group: 1 mL sample solution + 3 mL DPPH solution.

[0051] After mixing, react in the dark for 30 min, and then measure the absorbance at a wavelength of 517 nm (UV-Vis spectrophotometer, Agilent Cary 60).

[0052] 3. Calculation formula:

[0053] II. Mouse Liver SOD Activity Assay Reagent kit source: Nanjing Jiancheng Bioengineering Institute (Catalog No.: A001-3); Reference standard: Reagent kit instruction manual. The experiment used a randomized block design, with three replicates for each of Examples 1-5 and Comparative Examples 1-3. Data are expressed as mean ± standard deviation.

[0054] Experimental steps 1. Sample preparation Animal model: Male ICR mice (20±2g, SPF grade) were euthanized after 7 days of acclimatization and their livers were harvested.

[0055] Liver homogenization: Under ice bath conditions, homogenize at 10,000 rpm for 30 seconds using an IKA T10 Basic homogenizer, centrifuge at 12,000 rpm for 15 minutes at 4°C, and collect the supernatant.

[0056] 2. Measurement Procedure Prepare the chromogenic reagent, substrate solution, and enzyme working solution according to the kit instructions.

[0057] Add the following sequentially to the 96-well plate: Well 1: 20 μL sample + 140 μL substrate solution, incubate at 37°C for 20 min; Well 2: 20 μL sample + 60 μL chromogenic reagent + 60 μL enzyme working solution, incubate at 37°C for 40 min.

[0058] Terminate the reaction: Add 50 μL of stop solution and immediately measure the absorbance at 450 nm. Measure the absorbance using a microplate reader (BioTek Synergy H1).

[0059] 3. Calculation formula

[0060] Note: Unit: U / mL, one activity unit is defined as the inhibition of 50% of free radicals.

[0061] The experimental results are shown in Table 1.

[0062] Table 1. DPPH free radical scavenging rate and mouse liver SOD activity

[0063] *Note: Compared with Example 1, *p<0.05, **p<0.01 (t-test).

[0064] In summary, the anti-aging capsules prepared by the method and raw materials specified in this application can improve the absorption efficiency of PQQ, and when combined with other effective ingredients, can improve the DPPH free radical scavenging rate and SOD activity.

[0065] The DPPH scavenging rate and SOD activity of Examples 1-3 were significantly higher than those of Comparative Example 2, indicating that nanotechnology enhances antioxidant efficacy by improving the bioavailability of PQQ and the stability of liposomes. The DPPH scavenging rate of Example 4 decreased by 27.3% (*p<0.05), which was attributed to liposome aggregation caused by particle size exceeding the specified range, reducing the exposure efficiency of active sites. The SOD activity of Example 5 decreased by 19.8% (*p<0.05), which was attributed to the disruption of the liposome bilayer structure by excessive phospholipids, weakening encapsulation efficiency and mitochondrial targeting. The SOD activity of Comparative Example 3 was only 25.1 U / mL, a decrease of 48.3% compared to Example 1 (**p<0.01), indicating that anthocyanins and PQQ have a good complementary effect in free radical scavenging and mitochondrial repair.

[0066] The various embodiments in this specification are described in a progressive manner. Similar or identical parts between embodiments can be referred to interchangeably. Each embodiment focuses on describing the differences from other embodiments. In particular, the system embodiments are basically similar to the method embodiments, so the description is relatively simple; relevant parts can be referred to the descriptions in the method embodiments.

[0067] The above description is merely an embodiment of this application and is not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. An anti-aging soft capsule, characterized in that, It is prepared from the following raw materials: 40-50 parts anthocyanin extract, 15-20 parts pyrroloquinoline quinone, 80-85 parts soybean lecithin, 820-830 parts fish oil, 20-30 parts beeswax, 100-120 parts gelatin, 50-60 parts glycerin and 100-120 parts water; The pyrroloquinoline quinone and soybean phospholipids are nano-sized to form nanoliposomes.

2. The anti-aging soft capsule according to claim 1, characterized in that, The nanoliposomes have a particle size of 20-200 nm, an encapsulation efficiency of not less than 85%, and a drug loading of not less than 15%.

3. The anti-aging soft capsule according to claim 1, characterized in that, The weight ratio of the pyrroloquinoline quinone to the soybean lecithin is 1:(4-5).

4. The anti-aging soft capsule according to claim 1, characterized in that, The method for preparing the nanoliposomes includes the following steps: a. Dissolve soybean lecithin in chloroform and pyrroloquinoline quinone in phosphate buffer; b. Under magnetic stirring, pyrroloquinoline quinone buffer solution was added dropwise to soybean lecithin chloroform solution, and stirring was continued to obtain colostrum; c. Transfer the colostrum to a rotary evaporator and remove chloroform by rotary evaporation, stirring continuously until a translucent nanoliposome solution is obtained; d. The nanoliposome solution is repeatedly ultrafiltered through an ultrafiltration membrane to remove unencapsulated material until the particle size is 20-200 nm, thus obtaining the nanoliposomes.

5. The anti-aging soft capsule according to claim 4, characterized in that, In step b, the stirring speed is 500-800 rpm; the dropping rate is 0.5-1 mL / s; and the stirring time is 30-40 min.

6. The anti-aging soft capsule according to claim 4, characterized in that, The rotary evaporation conditions in step c are: temperature 40-45℃, vacuum degree 0.08-0.10MPa, and rotation speed 50-100rpm.

7. The anti-aging soft capsule according to claim 1, characterized in that, The fish oil contains no less than 60% ω-3 polyunsaturated fatty acids.

8. A method for preparing an anti-aging soft capsule as described in any one of claims 1-7, characterized in that, Includes the following steps: (1) Weigh fish oil and beeswax, heat to dissolve, and cool to room temperature to form an oil-wax liquid; (2) After filtering and heat preservation, gelatin, glycerin and hydrated gelatin are added; (3) Mix the oil and wax liquid obtained in step (1) with the glue liquid obtained in step (2), anthocyanin extract, and PQQ-soybean phospholipid nanoliposomes evenly, let stand, and then press into pellets to obtain the anti-aging soft capsules.

9. The preparation method according to claim 8, characterized in that, In step (1), the heating temperature is 65℃-75℃. In step (2), after the gel is dissolved, it is filtered through a 100-mesh filter and the sol temperature is 60℃-80℃.

10. The preparation method according to claim 8, characterized in that, In step (3), the anthocyanin extract and PQQ-soybean phospholipid nanoliposomes are sieved through an 80-mesh sieve and then mixed with oil wax liquid and glue liquid.

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