A process for simultaneously extracting anticoagulant active substances from leeches

Abstract

The present application relates to the field of living extraction technology, in particular to a leech living body collection and anticoagulant activity substance synchronous extraction process, which comprises the following steps: a large number of living leeches are poured into a shunt box and are shunted by a shunt channel and then fall into a row of a plurality of object cavities; a motor drives a transfer cylinder to rotate intermittently to pick up the living leeches, and the transfer cylinder adsorbs the leeches; when the leeches fall into a plurality of collection cavities of a collection box, the leeches are attracted by a blood-containing artificial membrane and bite, and the saliva is secreted by the current stimulation of an electrode sheet; the saliva flows into a liquid collecting box and is filtered by a filter membrane in the liquid collecting box, the filtered saliva is directly introduced into a low-temperature container through a bottom port connecting pipeline of the liquid collecting box for collection, and the leech anticoagulant is obtained by centrifugal impurity removal. The funnel structure of the shunt box is combined with the flow guide sleeve to increase the single processing capacity, the air pressure adsorption and the intermittent rotation mechanism of the transfer cylinder are used to complete individual transfer, and the electrode sheet and the artificial membrane integrated stimulation system are used to achieve directional saliva collection.

Description

Technical Field

[0001] This invention relates to the field of live extraction technology, specifically a process for the simultaneous harvesting of live leeches and extraction of anticoagulant active substances. Background Technology

[0002] Hirudin, a natural anticoagulant, has significant value in the pharmaceutical field, and its extraction process directly affects the purity and activity of the product. Traditional extraction methods mainly rely on collecting salivary glands from live leeches in water. Operators need to place starved medical leeches on specific sites and stimulate them to secrete saliva through physical stimulation.

[0003] The invention disclosed in application number CN201510956212.1 is a hirudin extraction device and extraction method, including a collection tank, a water pump, a tray, and a feeding sleeve. The tray is suspended in the leech breeding pond. The collection tank is set on one side of the tray. A water pump is set at the bottom of the collection tank. A discharge pipe is connected to the top of the water pump. The discharge pipe is fixed to the outer wall of the collection tank and extends outward to the top of the tray. An inlet pipe is also fixed to the outer wall of the collection tank on the side opposite to the discharge pipe. The tray is fixed below the inlet pipe and the discharge pipe by a fixing frame. The tray is used to support the U-shaped feeding sleeve that connects the discharge pipe and the inlet pipe.

[0004] The aforementioned existing technology induces leeches to suck blood and simultaneously secrete saliva through a circulating blood plasma via a sheep intestine cannula. While this method avoids physical harm to the leeches, it still has the following limitations:

[0005] Relying on manual placement of leeches results in a limited processing capacity per unit time and requires a half-hour feeding cycle, leading to low efficiency. Saliva separation is difficult, and the mixture of hirudin and residual plasma requires secondary freezing dehydration and irradiation sterilization, resulting in a high rate of activity loss. Furthermore, the high degree of manual labor dependence, from leech attraction and cannula coating to the recovery of leaked plasma, all require manual operation, making it difficult to achieve continuous industrial production.

[0006] Furthermore, modern biotechnology is driving innovation in extraction processes and research into gene recombination technology. Hirudin gene fragments are introduced into an *E. coli* expression system, and engineered bacteria are cultured on a large scale in fermenters. This process improves the efficiency of traditional physical stimulation, but it requires strict induction conditions, involving temperature control, concentration adjustment, and dissolved oxygen maintenance to ensure correct protein folding. Clearly, this is not something that most companies can achieve at any cost. The rapid iteration of this technology necessitates a gradual approach requiring companies to keep up. Therefore, until then, physical stimulation of leeches to secrete saliva is the preferred method, mainly because the efficiency of single-collection needs improvement. Summary of the Invention

[0007] To overcome the shortcomings of the existing technology, the present invention aims to provide a process for simultaneous extraction of live leeches and anticoagulant active substances. This process breaks through the bottleneck of large-scale production with a fully automated production line architecture. Through three core modules—physical diversion, air pressure transfer, and targeted electrical stimulation—saliva collection efficiency is improved while ensuring minimal damage to leeches, thereby solving the problems mentioned in the background technology.

[0008] To achieve the above objectives, this invention provides a process for simultaneous live leech harvesting and extraction of anticoagulant active substances, employing a diversion box to divert a large number of leeches, a transfer group to individually transfer a number of the diverted leeches, and a collection group to individually collect saliva from a number of leeches; including the following steps:

[0009] S1. A large number of live leeches are poured into the diversion box and diverted by the diversion channel, and fall into a row of storage chambers at the top of the transfer cylinder;

[0010] S2. Simultaneously start the air pump and motor. The motor drives the transfer cylinder to rotate intermittently to pick up live leeches. The air pump draws air from inside the transfer cylinder to create a state where leeches are adsorbed.

[0011] S3. When the initial row of several placement chambers of the transfer tube, which is adsorbed with leeches, rotates to the bottom, the suction effect is blocked by the sealing rod, and the leeches fall into several collection chambers of the collection box.

[0012] S4. At this time, the leeches in the collection chamber are attracted and bitten by the blood-stained artificial membrane, which triggers the current stimulation of the electrode plate and causes them to secrete saliva.

[0013] S5. Push the pusher out of the collection box, then replace it with a new collection box and place it under the transfer tube to collect leeches, so that they can be stimulated to secrete saliva through electric current.

[0014] S6. The saliva secreted by the leeches flows into the collection box and is filtered by the filter membrane inside the box. The filtered saliva is then directly introduced into a low-temperature container through the pipe connected to the bottom port of the collection box for collection. After centrifugation to remove impurities, the leeches are collected and transferred to the breeding area.

[0015] As a further improvement to this technical solution, the diversion box is a Y-shaped funnel-shaped box with several diversion plates evenly spaced on its inner wall. A guide sleeve is inserted between each adjacent diversion plate, further dividing the adjacent diversion plates into several diversion channels. The transfer assembly includes a transfer cylinder placed below the bottom of the guide sleeve, an air pump sealed to one end of the transfer cylinder, a motor for driving the rotation of the transfer cylinder, and a sealing rod inserted into the central shaft of the transfer cylinder. The outer wall of the transfer cylinder is provided with several storage compartments by sleeved with annular mesh silicone tubes. The transfer cylinder has a vent pipe on its central axis, and several gas distribution pipes are sleeved between the transfer cylinder and the vent pipe, with each gas distribution pipe corresponding to a number of storage cavities. The collection group includes a collection box placed directly below the transfer cylinder, liquid collection groups on both sides of the collection box, and a pusher for supporting the movement of the collection box. Several collection cavities are provided on the center line of the collection box, which correspond to a row of several storage cavities. The liquid collection group consists of several liquid collection boxes and several electrode plates, and an artificial membrane is inserted into the top of the liquid collection box.

[0016] The above setup takes into account the high difficulty and cost of gene culture technology, which prevents traditional enterprises from making significant leaps in improvement. Therefore, it also starts with the extraction of hirudin through physical stimulation. By using a fully automated production line architecture to overcome the bottleneck of large-scale production, and through three core modules of physical diversion, air pressure transfer, and targeted electrical stimulation, the efficiency of saliva collection is improved and the manual intervention is reduced while ensuring zero damage to leeches. This provides a technical foundation for the large-scale supply of anticoagulant drugs.

[0017] As a further improvement to this technical solution, the bottom of the diversion box is connected to a ground support box, the two ends of the vent pipe are sleeved and rotated with the two ends of the top of the ground support box, the air pump suction end is sleeved and fitted with the vent pipe through a bearing, and the motor is connected to the outer end of the vent pipe through a transmission assembly.

[0018] As a further improvement to this technical solution, the transmission component is a worm gear transmission structure or a pulley belt transmission structure.

[0019] These two settings address the process requirements of ensuring stable rotation of the transfer cylinder and efficient air pump suction.

[0020] As a further improvement to this technical solution, a flow inlet is provided on the lower side wall of the top of the support box, and a flow inlet plate is fixedly provided at the bottom edge of the flow inlet in a downward-sloping state. A collection trough is suspended below the flow inlet plate; a flow guide frame is fixedly provided on the top of several flow guide sleeves.

[0021] This feature allows leeches that are not sucked in by air to flow out, and then they are collected and poured back into the distribution box.

[0022] As a further improvement to this technical solution, the width of the sealing rod is equal to the outer diameter of the air distribution pipe. One end of the sealing rod is fitted with a round cap, which is inserted into the end face of the ground support box by a pin. The outer diameter of this round cap is larger than the inner diameter of the air pipe. The other end of the sealing rod is fixed with a circular ring, which is fitted with the inner wall of the air pipe port with a gap.

[0023] As a further improvement to this technical solution, the collection box is symmetrically provided with liquid collection chambers on both sides, the electrode plate is symmetrically provided with electrode posts on both sides, the liquid collection box and the electrode posts penetrate the bottom surface of the liquid collection chamber, the central axis of the liquid collection box is provided with an insertion post, the upper port of the liquid collection chamber is snapped with a tray, and the insertion post and the tray are inserted and matched.

[0024] As a further improvement to this technical solution, a number of battery modules are fixedly provided on the bottom surface of the acquisition box, and a number of electrode posts are provided on both sides of the battery modules, with the electrode posts in corresponding contact with the electrode posts.

[0025] These two features, through modular design, integrate the electrode pads and artificial membrane into a stimulation system to achieve targeted saliva collection and impurity isolation.

[0026] As a further improvement to this technical solution, a round shaft is inserted into the center line of the top port of the acquisition cavity, and a flip cover is rotatably connected to the top port of the acquisition cavity. A retaining strip is provided on the center line of the bottom surface of the flip cover. The retaining strip is a C-shaped block and is engaged with the round shaft for rotation.

[0027] As a further improvement to this technical solution, the two ends of the support box are open, and the middle and lower parts of the support box are open, and the bottom corners of the push frame are equipped with casters.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] 1. This process for simultaneous live leech harvesting and extraction of anticoagulant active substances utilizes a funnel structure and guide sleeve combination in the diversion box to achieve zero-damage physical grading of leeches, increasing the single-batch processing capacity while avoiding the operational burden caused by manual sorting. Through the air pressure adsorption and intermittent rotation mechanism of the transfer cylinder, leeches are accurately dropped into the collection chamber in batches, achieving individualized transfer. Through the integrated stimulation system of electrode plates and artificial membranes, saliva is collected in a targeted manner and impurities are isolated. Saliva is directly separated through the built-in filter membrane in the collection box, eliminating the need for secondary purification.

[0030] 2. The process of simultaneously collecting live leeches and extracting anticoagulant active substances uses a modular collection box and a moving pusher design to achieve parallel collection and transfer processes. The liquid collection group can be quickly disassembled and replaced to ensure continuous production. The pusher carries the collection box to shuttle through the production line, which increases the daily processing capacity of a single device compared to traditional processes. Attached Figure Description

[0031] The accompanying drawings described herein are for illustrative purposes only and are not intended to limit the scope of the invention in any way. Furthermore, the shapes and proportions of the components in the drawings are merely illustrative to aid in understanding the invention and do not specifically limit the shapes and proportions of the components. Those skilled in the art, guided by the teachings of this invention, will select various possible shapes and proportions to implement the invention according to specific circumstances.

[0032] Figure 1 This is a schematic diagram of the overall assembly structure of the present invention;

[0033] Figure 2 For the present invention Figure 1 Side view;

[0034] Figure 3 This is a schematic diagram of the overall internal assembly structure of the present invention;

[0035] Figure 4 For the present invention Figure 3 The main view;

[0036] Figure 5 This is a schematic diagram of the flow divider structure of the present invention;

[0037] Figure 6 This is a breakdown diagram of the transfer assembly of the present invention;

[0038] Figure 7 This is a schematic diagram of the data acquisition group assembly structure of the present invention;

[0039] Figure 8 This is a split view of the data collection box of the present invention;

[0040] Figure 9 This is a split diagram of the liquid collection assembly of the present invention;

[0041] Figure 10 This is a front view of the air guide frame of the present invention;

[0042] Figure 11 This is a front view of the transfer assembly of the present invention;

[0043] Figure 12 This is a front view of the liquid collection assembly of the present invention;

[0044] The meanings of the labels in the diagram are as follows:

[0045] 100. Diverter box; 101. Diverter plate; 102. Support box; 103. Inlet; 110. Flow guide frame; 111. Flow guide sleeve; 120. Flow guide plate; 130. Collection tank;

[0046] 200. Transfer assembly; 210. Transfer cylinder; 211. Storage chamber; 212. Air distribution pipe; 213. Ventilation pipe; 220. Air pump; 230. Motor; 240. Sealing rod;

[0047] 300. Collection group; 310. Collection box; 311. Collection chamber; 312. Flip cover; 3121. Locking strip; 313. Liquid collection chamber; 314. Tray; 320. Liquid collection group; 321. Liquid collection box; 3211. Insertion post; 322. Electrode plate; 3221. Electrode post; 323. Battery module; 324. Connection post; 330. Push frame. Detailed Implementation

[0048] The details of the present invention can be more clearly understood by referring to the accompanying drawings and the description of specific embodiments. However, the specific embodiments of the present invention described herein are for illustrative purposes only and should not be construed as limiting the invention in any way. Under the teachings of this invention, those skilled in the art will conceive of any possible variations of the invention, all of which should be considered within the scope of the invention. The terms "installation" and "connection" should be interpreted broadly, referring to direct connection as well as indirect connection through an intermediate medium.

[0049] The terms "central axis," "vertical," "horizontal," "front," "rear," "upper," "lower," "left," "right," "top," "bottom," "inner," and "outer" used herein to indicate orientation or positional relationships are based on the orientation or positional relationships shown in the accompanying drawings and are used only for the convenience of describing the invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, in the description of the invention, "a number" means two or more, unless otherwise explicitly specified.

[0050] Please see Figures 1-12 As shown, this invention provides a process for simultaneous extraction of live leeches and anticoagulant active substances. It employs a diversion box 100 to divert a large number of leeches, a transfer group 200 to individually transfer a number of the diverted leeches, and a collection group 300 to individually collect saliva from a number of leeches. This achieves industrial-scale production, enabling large-scale production and providing reliable raw materials for the development of anticoagulant drugs. The diversion box 100 is a Y-shaped funnel with several diversion plates 101 evenly spaced on its inner wall. A guide sleeve 111 is inserted between each adjacent diversion plate 101, further dividing the adjacent diversion plates 101 into several diversion channels. First, a large number of leeches are diverted through the diversion plates 101, and then a small number of leeches are further subdivided through the diversion channels, preparing for subsequent individual saliva collection.

[0051] The transfer assembly 200 includes a transfer cylinder 210 located below the bottom of the guide sleeve 111, an air pump 220 sealed to one end of the transfer cylinder 210, a motor 230 for driving the rotation of the transfer cylinder 210, and a sealing rod 240 inserted into the central shaft of the transfer cylinder 210. The outer wall of the transfer cylinder 210 is provided with several storage cavities 211 by sleeved with an annular mesh-like silicone tube. This material is soft and durable, providing a cushioning effect so that leeches can fall in without injury; and so that the... The diverted leeches are separated and transferred again; a vent pipe 213 is provided on the central axis of the transfer cylinder 210, and several air distribution pipes 212 are sleeved between the transfer cylinder 210 and the vent pipe 213. The air distribution pipes 212 correspond one-to-one with several storage cavities 211, so that the leeches falling into the storage cavity 211 are sucked in by air, while the leeches that are not sucked in by air slide out of the storage cavity 211 during the downward tilting of the transfer cylinder 210, so that they can be collected and poured into the diversion box 100 for further diversion.

[0052] Furthermore, the bottom of the diversion box 100 is connected to a support box 102. The two ends of the vent pipe 213 are sleeved and rotated with the two ends of the top of the support box 102. The top of the support box 102 has round holes at both ends, allowing the transfer cylinder 210 to pass through. A round cover is fixedly connected to the round hole by bolts to support the rotation of the transfer cylinder 210. The suction end of the air pump 220 is sleeved with the vent pipe 213 through a bearing to prevent the rotation of the transfer cylinder 210 from interfering with the stable operation of the air pump 220. The motor 230 is connected to the outer end of the vent pipe 213 through a transmission assembly. The transmission assembly is a worm gear transmission structure or a pulley belt transmission structure, which is existing technology and will not be described in detail here. This drives the transfer cylinder 210 to rotate intermittently at a fixed angle, while continuously cooperating in the process of collecting and releasing leeches.

[0053] Furthermore, a drainage port 103 is provided on the lower side wall of the top of the support box 102. The drainage port 103 is located in the lower middle part of the transfer cylinder 210 so that leeches that are not sucked out of the storage cavity 211 can slide out. A drainage plate 120 is fixedly provided at the bottom edge of the drainage port 103 in a downward tilting state. A collection trough 130 is suspended below the drainage plate 120 to guide the leeches to slide into the collection trough 130 for temporary collection. A guide frame 110 is fixedly provided on the top of several guide sleeves 111. The guide frame 110 is engaged with the top port of the diversion box 100 so as to be picked up and put down as a whole.

[0054] Furthermore, the width of the sealing rod 240 is equal to the outer diameter of the air distribution pipe 212, so as to seal the air distribution pipe 212 channel at the bottom of the transfer cylinder 210, so that the leeches in the storage cavity 211 lose their suction and fall down; one end of the sealing rod 240 is fitted with a round cover, and the round cover is inserted into the end face of the support box 102 by a pin to prevent the sealing rod 240 from rotating and shifting; the outer diameter of this round cover is larger than the inner diameter of the vent pipe 213, so as to achieve the sealing effect; the other end of the sealing rod 240 is fixedly fitted with a ring, and the ring is fitted with the inner wall of the vent pipe 213 port with a gap, so that the sealing rod 240 is stably supported and does not rotate with the transfer cylinder 210.

[0055] Specifically, such as Figures 7-9 As shown, the collection group 300 includes a collection box 310 placed directly below the transfer cylinder 210, a liquid collection group 320 arranged on both sides of the collection box 310, and a pusher 330 for supporting the movement of the collection box 310; the collection box 310 has a number of collection chambers 311 on its center line, which correspond one-to-one with a row of a number of placement chambers 211, so as to collect leeches falling from the placement chambers 211.

[0056] The collection assembly 320 consists of several collection boxes 321 and several electrode plates 322. An artificial membrane is inserted into the top of the collection box 321. The collection box 310 has symmetrical collection chambers 313 on both sides, and electrode posts 3221 are symmetrically arranged on both sides of the electrode plates 322. The collection box 321 and the electrode posts 3221 penetrate the bottom surface of the collection chamber 313. The central axis of the collection box 321 has an insertion post 3211. Several battery modules 323 are fixedly arranged on the bottom surface of the collection box 310. Several docking posts 324 are arranged on both sides of the battery modules 323. The electrode posts 3221 are in contact with the docking posts 324, so that current can pass through the electrode plates 322. When the leech bites the artificial membrane, it is stimulated by the current of the electrode plates 322 and secretes saliva.

[0057] Furthermore, a tray 314 is snapped onto the upper port of the liquid collection chamber 313, and the insertion post 3211 is inserted into the tray 314 to facilitate the overall placement and removal of the liquid collection assembly 320, thereby enabling modular operation.

[0058] A round shaft is inserted into the center line of the top port of the collection cavity 311. A flip cover 312 is rotatably connected to the top port of the collection cavity 311. A retaining strip 3121 is provided on the center line of the bottom surface of the flip cover 312. The retaining strip 3121 is a C-shaped block and is engaged with the round shaft for rotation. This allows leeches falling onto the flip cover 312 to open the gap due to imbalance, and the leeches will slide into the collection cavity 311, thus receiving a buffering effect.

[0059] The support box 102 has open ends and is located in the middle and slightly below. The bottom corner of the pusher 330 is equipped with casters, which allow the pusher 330 to carry the collection group 300 under the transfer tube 210 to complete the work of alternately collecting leeches and collecting saliva.

[0060] The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances of the present invention includes the following steps:

[0061] S1. A large number of live leeches are poured into the diversion box 100 and diverted by the diversion channel, and fall into a row of storage cavities 211 at the top of the transfer cylinder 210;

[0062] S2. Simultaneously start the air pump 220 and the motor 230. The motor 230 drives the transfer cylinder 210 to rotate intermittently to pick up live leeches. The air pump 220 draws air from inside the transfer cylinder 210 to create a state where leeches are adsorbed. This causes the leeches to be sucked in by the air and not fall off when the transfer cylinder 210 tilts down. The leeches that are not sucked in by the air slide out from the side and are collected and poured back into the top of the diversion box 100 to continue diversion.

[0063] S3. When the initial row of several placement chambers 211 of the transfer tube 210, which adsorbs leeches, rotates to the bottom, the suction effect is blocked by the sealing rod 240, and the leeches fall into several collection chambers 311 of the collection box 310; thus, each placement chamber 211 releases only one leech to collect saliva.

[0064] S4. At this time, the leech in the collection chamber 311 is attracted and bitten by the blood-stained artificial membrane, which triggers the current stimulation of the electrode 322 to secrete saliva. The nerves around the leech's mouthparts are briefly stimulated by a low-intensity current, usually a DC or pulsed current of 1-5V. This current simulates the temperature or vibration signal of the host's skin when the leech naturally sucks blood, triggering the reflexive secretion of its salivary glands.

[0065] S5, then push the pusher 330 out of the collection box 310, and then replace it with a new collection box 310 and place it under the transfer tube 210 to collect leeches, so that they can secrete saliva through electric current stimulation; thus continuously collecting leeches in each row of several placement chambers 211 of the transfer tube 210 to secrete saliva.

[0066] S6. The saliva secreted by the leeches flows into the collection box 321 and is filtered by the filter membrane inside the box. The filtered saliva is directly introduced into a low-temperature container for collection through the pipe connected to the bottom port of the collection box 321. After centrifugation to remove impurities, hirudin, an anticoagulant active substance, is obtained. Subsequently, the leeches are removed from the collection box 310 and transferred to the breeding area.

[0067] It should be noted that the fixed connections and fixing methods of the present invention are achieved using conventional fixing means such as bolt connections or welding. The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and should not be used to limit the scope of protection of the present invention. All equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A process for simultaneous extraction of live leeches and anticoagulant active substances, comprising using a diversion box to divert a large number of leeches, using a transfer group to individually transfer a number of leeches after diversion, and using a collection group to individually collect saliva from a number of leeches; characterized in that, Includes the following steps: S1. A large number of live leeches are poured into the diversion box and diverted by the diversion channel, and fall into a row of storage chambers at the top of the transfer cylinder; S2. Simultaneously start the air pump and motor. The motor drives the transfer cylinder to rotate intermittently to pick up live leeches. The air pump draws air from inside the transfer cylinder to create a state where leeches are adsorbed. S3. When the initial row of several placement chambers of the transfer tube, which is adsorbed with leeches, rotates to the bottom, the suction effect is blocked by the sealing rod, and the leeches fall into several collection chambers of the collection box. S4. At this time, the leeches in the collection chamber are attracted and bitten by the blood-stained artificial membrane, which triggers the current stimulation of the electrode plate and causes them to secrete saliva. S5. Push the pusher out of the collection box, then replace it with a new collection box and place it under the transfer tube to collect leeches, so that they can be stimulated to secrete saliva through electric current. S6. The saliva secreted by the leeches flows into the collection box and is filtered by the filter membrane inside the box. The filtered saliva is directly introduced into a low-temperature container through the pipe at the bottom port of the collection box for collection. It is then centrifuged to remove impurities and obtain hirudin. Afterwards, the leeches are removed from the collection box and transferred to the breeding area. The diversion box is a Y-shaped funnel-shaped box with several diversion plates evenly spaced on its inner wall. A guide sleeve is inserted between each adjacent diversion plate, further dividing the adjacent diversion plates into several diversion channels. The transfer group includes a transfer cylinder placed below the bottom of the guide sleeve, an air pump sealed to one end of the transfer cylinder, a motor for driving the rotation of the transfer cylinder, and a sealing rod inserted into the central axis of the transfer cylinder. The outer wall of the transfer cylinder has several storage cavities through a ring-shaped mesh silicone tube. A vent pipe is provided on the central axis of the transfer cylinder. Several gas distribution pipes are sleeved between the transfer cylinder and the vent pipe, and the gas distribution pipes correspond one-to-one with several storage cavities. The collection group includes a collection box placed directly below the transfer cylinder, liquid collection groups on both sides of the collection box, and a pusher for supporting the movement of the collection box. Several collection cavities are provided on the center line of the collection box, which correspond one-to-one with a row of several storage cavities. The liquid collection group consists of several liquid collection boxes and several electrode plates. An artificial membrane is inserted through the top of the liquid collection box. The collection box has symmetrical liquid collection chambers on both sides, and the electrode plates have symmetrical electrode posts on both sides. The liquid collection box and the electrode posts penetrate the bottom surface of the liquid collection chamber. The central axis of the liquid collection box has an insertion post, and the upper port of the liquid collection chamber is snapped with a tray. The insertion post and the tray are inserted into each other. The bottom surface of the acquisition box is fixedly provided with several battery modules, and several docking posts are provided on both sides of the battery modules, with the electrode posts in corresponding contact with the docking posts.

2. The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances according to claim 1, characterized in that: The bottom of the diversion box is connected to a ground support box. The two ends of the vent pipe are sleeved and rotated with the two ends of the top of the ground support box. The air pump suction end is sleeved and fitted with the vent pipe through a bearing. The motor is connected to the outer end of the vent pipe through a transmission assembly.

3. The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances according to claim 2, characterized in that: The transmission component is a worm gear transmission structure or a pulley belt transmission structure.

4. The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances according to claim 3, characterized in that: The bottom side wall of the support box is provided with a flow outlet, and a flow guide plate is fixedly installed at the bottom edge of the flow outlet in a downward tilting state. A collection trough is suspended below the flow guide plate; a flow guide frame is fixedly installed on the top of several flow guide sleeves.

5. The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances according to claim 4, characterized in that: The width of the sealing rod is equal to the outer diameter of the air distribution pipe. One end of the sealing rod is fitted with a round cap, which is inserted into the end face of the support box by a pin. The outer diameter of this round cap is larger than the inner diameter of the air pipe. The other end of the sealing rod is fixed with a circular ring, which is fitted with the inner wall of the air pipe port with a gap.

6. The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances according to claim 5, characterized in that: A round shaft is inserted into the center line of the top port of the acquisition cavity. A flip cover is rotatably connected to the top port of the acquisition cavity. A retaining strip is provided on the center line of the bottom surface of the flip cover. The retaining strip is a C-shaped block and is engaged with the round shaft for rotation.

7. The process for simultaneous harvesting of live leeches and extraction of anticoagulant active substances according to claim 6, characterized in that: The two ends of the ground support box are open, and the middle and lower parts of the box are open. The bottom corners of the push frame are equipped with casters.

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