A kind of in-situ integrated urinary calculus detection device and method based on laser multispectral

Abstract

The application relates to a laser multispectral in-situ integrated urinary stone detection device and method, and relates to the technical field of urinary stone detection. The detection device comprises a sample platform, a microscopic LIBS mechanism comprising a microscopic objective lens and a LIBS collection head, the microscopic objective lens and the LIBS collection head both being oriented towards the sample platform, a composite light path mechanism, a Raman spectrum generation mechanism for generating continuous laser, a Raman spectrum collection mechanism, a Raman spectrum signal sequentially passing through the microscopic objective lens and the composite light path mechanism and then entering the Raman spectrum collection mechanism, a LIBS spectrum generation mechanism for generating pulsed laser, a LIBS spectrum collection mechanism, the LIBS collection head being used for collecting a LIBS spectrum signal and inputting the LIBS spectrum signal into the LIBS spectrum collection mechanism, and further comprising a control analysis mechanism. The application can realize early screening of urinary stones.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of urinary stone detection, and in particular to a urinary stone detection device and method based on laser multispectral in-situ integration. BACKGROUND

[0002] Urinary stones are a common urinary system disease, and the high incidence and recurrence rate have brought heavy economic burden to patients and society.

[0003] At present, computer tomography is often used in clinics to diagnose urinary stones, and computer tomography can screen urinary stones in advance. However, computer tomography lacks specificity in distinguishing stones of different chemical compositions, for example, computer tomography cannot distinguish calcium oxalate stones from infectious stones.

[0004] Since the existing analysis method of urinary stone composition can only be performed after the patient receives surgery and the stone is removed, it cannot accurately detect the composition of the urinary stone before surgery, and it is difficult to provide guidance for the development of a treatment plan before surgery, so the existing analysis of urinary stone composition has obvious hysteresis.

[0005] In view of the above-mentioned defects of the related art, in view of the metabolic richness and ease of collection of urine, detecting urinary stone information by detecting urine biomarkers becomes an attractive non-invasive solution, and at present, the detection of urinary stone composition according to urine is mainly through 24-hour urine detection method, which requires continuous collection of urine within 24 hours to analyze the composition in the 24-hour urine, which results in a long detection time and a lack of timeliness of the detection result. SUMMARY

[0006] The present application provides a urinary stone detection device and method based on laser multispectral in-situ integration, which aims to realize early screening of urinary stones by using urine samples, and timely confirm the chemical composition and element composition of urinary stones, so as to timely determine the type of urinary stones before surgery, thereby providing guidance for the development of a treatment plan before surgery.

[0007] In a first aspect, the urinary stone detection device based on laser multispectral in-situ integration provided by the present application adopts the following technical solution:

[0008] The application discloses a kind of based on laser multispectral in-situ integrated urinary calculus detection device, including sample platform;Microscopic LIBS mechanism, including microscopic objective and LIBS collection head, the microscopic objective and the LIBS collection head are all towards the sample platform;Composite optical path mechanism;Raman spectrum generation mechanism is used to generate continuous laser;Continuous laser is irradiated to the sample platform after sequentially passing through the composite optical path mechanism and the microscopic objective, for generating Raman spectrum signal;Raman spectrum acquisition mechanism, Raman spectrum signal enters the Raman spectrum acquisition mechanism after sequentially passing through the microscopic objective and the composite optical path mechanism;LIBS spectrum generation mechanism is used to generate pulsed laser, pulsed laser is irradiated to the sample platform after sequentially passing through the composite optical path mechanism and the microscopic objective, for generating LIBS spectrum signal;LIBS spectrum acquisition mechanism, the LIBS collection head is used to collect LIBS spectrum signal and input the LIBS spectrum acquisition mechanism;Further including control analysis mechanism, the Raman spectrum generation mechanism, LIBS spectrum generation mechanism, Raman spectrum acquisition mechanism and LIBS spectrum acquisition mechanism are electrically connected with the control analysis mechanism.

[0009] By adopting the above technical scheme, the sample platform loads the sample to be measured, the continuous laser generated by the Raman spectrum generation mechanism is irradiated to the sample to be measured on the sample platform after sequentially passing through the composite optical path mechanism and the microscopic objective, so that the sample to be measured generates Raman spectrum signal, and the Raman spectrum acquisition mechanism collects the Raman spectrum signal to obtain the molecular component information of the sample to be measured;The pulsed laser generated by the LIBS spectrum generation mechanism is also irradiated to the sample to be measured after sequentially passing through the composite optical path mechanism and the microscopic objective, so that the sample to be measured generates plasma and then obtains LIBS spectrum signal, and the LIBS collection head collects the LIBS spectrum signal and inputs the LIBS spectrum acquisition mechanism to obtain the element composition information of the sample to be measured.The control analysis mechanism can analyze the two kinds of collected spectrum signals, and then determine the composition of the sample to be measured.

[0010] This design can obtain the Raman spectrum signal and the LIBS spectrum signal of the sample to be measured, realizes the complementarity of the Raman spectrum signal and the LIBS spectrum signal, makes the molecular component information and the element composition information of the sample to be measured complementary and mutually verified, can realize comprehensive collaborative detection of the element and molecular composition of urinary calculus, and then judges whether the patient has calculus and detects the type of calculus through the urine sample of the patient, realizes non-invasive early screening of urinary calculus.

[0011] Optionally, the microscopic LIBS mechanism further includes a microscope converter, the microscopic objective and the LIBS collection head are both arranged on the microscope converter, and the focusing point of the LIBS collection head coincides with the focusing point position of the microscopic objective.

[0012] By adopting the technical scheme, the focusing point positions of the microscopic objective lens and the LIBS collection head are coincided by arranging the microscopic objective lens and the LIBS collection head on the microscope converter, so that the synchronous focusing of the microscopic objective lens and the LIBS collection head is realized, thereby reducing the focusing difficulty of the detection device and improving the detection efficiency.

[0013] Optionally, the microscopic objective lens is arranged vertically, the sample platform is arranged below the microscopic objective lens, and the lower end of the microscopic objective lens is arranged opposite to the sample platform; the Z-axis driver is arranged on the sample platform and is used to drive the sample platform to move in the vertical direction; and the Z-axis driver is electrically connected with the control analysis mechanism.

[0014] By adopting the technical scheme, the microscopic objective lens is arranged vertically and the lower end of the microscopic objective lens is arranged opposite to the sample platform; the Z-axis driver arranged on the sample platform is electrically connected with the control analysis mechanism; and the control analysis mechanism can drive the Z-axis driver to work and make the sample platform move in the vertical direction. Based on the design, the focusing operation of the microscopic objective lens can be realized by adjusting the distance between the sample platform and the microscopic objective lens.

[0015] Since the position adjustment of the sample platform is realized by the Z-axis driver, the automatic adjustment of the position of the sample platform is realized, the focusing difficulty of the microscopic objective lens is reduced, and the accuracy and efficiency of the focusing of the microscopic objective lens are improved.

[0016] Optionally, the X-axis driver and the Y-axis driver are further arranged, the X-axis driver, the Y-axis driver and the Z-axis driver are sequentially connected, the driving directions of the X-axis driver and the Y-axis driver are arranged perpendicular to each other, the driving directions of the X-axis driver and the Y-axis driver are arranged perpendicular to the driving direction of the Z-axis driver, and the X-axis driver and the Y-axis driver are electrically connected with the control analysis mechanism.

[0017] By adopting the technical scheme, the X-axis driver, the Y-axis driver and the Z-axis driver are sequentially connected and arranged perpendicular to each other in the driving directions, and are electrically connected with the control analysis mechanism; and the control analysis mechanism can control the X-axis driver and the Y-axis driver to drive the sample platform to move in two dimensions.

[0018] On this basis, when the sample to be detected is detected, the position of the sample platform is changed, so that the microscopic objective lens and the LIBS collection head are focused on different positions of the sample to be detected on the sample platform, thereby realizing the multi-point detection of the urine sample.

[0019] When the number and density of the detection positions are further increased, the sample to be detected can be scanned in a plane, full-coverage scanning imaging and omnidirectional detection of the sample to be detected are realized, the accuracy and comprehensiveness of the detection result are improved, and the surface information of the solid sample in the sample to be detected can be scanned and imaged, and the distribution of elements and molecules is detected, which can improve the accuracy of the detection result.

[0020] Optionally, the device further comprises an automatic focusing assembly, the automatic focusing assembly, the composite light path mechanism and the microscope objective are sequentially arranged; the Raman spectrum signal sequentially passes through the microscope objective and the composite light path mechanism and enters the automatic focusing assembly and the Raman spectrum acquisition mechanism; the automatic focusing assembly is electrically connected with the control analysis mechanism.

[0021] By adopting the above technical scheme, the automatic focusing assembly, the composite light path mechanism and the microscope objective are sequentially arranged, and the Raman spectrum signal enters the automatic focusing assembly and the Raman spectrum acquisition mechanism after passing through the microscope objective and the composite light path mechanism.

[0022] On this basis, when the Raman spectrum signal passes through the composite light path mechanism, part of the Raman spectrum signal is reflected into the automatic focusing assembly, and the other part of the Raman spectrum signal enters the Raman spectrum acquisition mechanism, which does not affect the normal collection of the Raman spectrum signal and can also provide the Raman spectrum signal for the automatic focusing assembly.

[0023] When part of the Raman spectrum signal is reflected into the automatic focusing assembly, part of the Raman spectrum signal is automatically imaged in the automatic focusing assembly, and the generated image information is transmitted to the control analysis mechanism, the control analysis mechanism processes the image information, determines the focusing condition of the microscope objective, and drives the Z-axis driver according to the image information processing result, so as to adjust the sample platform position, realize the automatic focusing of the microscope objective and the LIBS acquisition head.

[0024] Under the cooperation of the Z-axis driver, the automatic focusing assembly and the control analysis mechanism, the focusing of the microscope objective and the LIBS acquisition head can be automatically realized, so as to greatly improve the detection efficiency and accuracy and avoid errors and time consumption caused by manual focusing.

[0025] Optionally, the composite light path mechanism comprises a trap filter; the Raman spectrum generation mechanism, the trap filter and the microscope objective are sequentially arranged along the transmission direction of the continuous laser, and the trap filter reflects the continuous laser; the Raman spectrum acquisition mechanism, the trap filter and the microscope objective are sequentially arranged along the transmission direction of the Raman spectrum signal, and the Raman spectrum signal passes through the trap filter.

[0026] By adopting the above technical scheme, the composite light path mechanism is provided with the notch filter, the notch filter is arranged between the Raman spectrum generating mechanism and the microscope objective along the transmission direction of the continuous laser, and the notch filter can reflect the continuous laser to the microscope objective; when the Raman spectrum signal is transmitted, the notch filter allows the Raman spectrum signal to pass through and enter the Raman spectrum acquisition mechanism.

[0027] Therefore, the notch filter realizes the reflection of the continuous laser and the penetration of the Raman spectrum signal, so that the Rayleigh scattering noise of the continuous laser can be effectively filtered out, the purity of the Raman spectrum signal can be ensured, and the accuracy of the Raman spectrum signal acquisition can be improved, which can optimize the transmission quality of the continuous laser and the Raman spectrum signal, and the design of the notch filter realizes the partial overlap of the Raman excitation light path and the Raman acquisition light path, so that the space utilization of the light path is improved, and the miniaturization and compactness of the device are further realized.

[0028] Optionally, the composite light path mechanism comprises a dichroic mirror; the Raman spectrum generating mechanism, the dichroic mirror and the microscope objective are sequentially arranged along the transmission direction of the continuous laser, and the dichroic mirror reflects the continuous laser; the LIBS spectrum generating mechanism, the dichroic mirror and the microscope objective are sequentially arranged along the transmission direction of the pulsed laser, and the pulsed laser passes through the dichroic mirror.

[0029] By adopting the above technical scheme, the composite light path mechanism is provided with the notch filter, the notch filter is arranged between the Raman spectrum generating mechanism and the microscope objective along the transmission direction of the continuous laser, and the notch filter can reflect the continuous laser to the microscope objective; when the Raman spectrum signal is transmitted, the notch filter allows the Raman spectrum signal to pass through and enter the Raman spectrum acquisition mechanism.

[0030] Optionally, the Raman spectrum generating mechanism, the Raman spectrum acquisition mechanism and the LIBS spectrum generating mechanism are provided with polarizers between the composite light path mechanism, and the continuous laser, the pulsed laser and the Raman spectrum signal all pass through the corresponding polarizers.

[0031] By adopting the technical scheme, the polarizer is arranged between the Raman spectrum generating mechanism, the Raman spectrum collecting mechanism and the LIBS spectrum generating mechanism and the composite light path mechanism, the continuous laser, the pulsed laser and the Raman spectrum signal all pass through the corresponding polarizer, the polarizer can control and adjust the polarization state of the continuous laser, the pulsed laser and the Raman spectrum signal, reduce the stray light interference, improve the purity and quality of the continuous laser, the pulsed laser and the Raman spectrum signal, and further improve the accuracy and reliability of the detection result of the urinary calculus detection device.

[0032] In a second aspect, the application provides a urinary calculus detection method based on laser multispectrum in-situ integration, which adopts the following technical scheme:

[0033] A urinary calculus detection method based on laser multispectrum in-situ integration, which utilizes the above-mentioned urinary calculus detection device based on laser multispectrum in-situ integration, comprises the following steps:

[0034] S1, collecting a urine sample and preparing a sample to be tested, and placing the sample to be tested on the sample platform; S2, focusing the microscope objective and the LIBS collecting head on the sample to be tested; S3, the Raman spectrum generating mechanism emits continuous laser, the continuous laser is sequentially irradiated to the sample to be tested after passing through the composite light path mechanism and the microscope objective, and the sample to be tested generates Raman spectrum signal; the Raman spectrum signal sequentially passes through the microscope objective and the composite light path mechanism and enters the Raman spectrum collecting mechanism; S4, the LIBS spectrum generating mechanism emits pulsed laser, the pulsed laser is sequentially irradiated to the sample to be tested after passing through the composite light path mechanism and the microscope objective, and the sample to be tested generates LIBS spectrum signal; the LIBS collecting head collects the LIBS spectrum signal and inputs it into the LIBS spectrum collecting mechanism; S5, adjusting the position of the sample platform, and repeating steps S3-S4 to obtain a plurality of groups of Raman spectrum signals and LIBS spectrum signals; S6, the control analysis mechanism acquires a plurality of groups of Raman spectrum signals and LIBS spectrum signals, and analyzes the composition of the sample to be tested according to the plurality of groups of Raman spectrum signals and LIBS spectrum signals; S7, determining whether the sample to be tested contains calculus or the type of calculus according to the composition of the sample to be tested.

[0035] By adopting the above technical scheme, the detection method can obtain the Raman spectrum signal and the LIBS spectrum signal generated at the same position of the sample to be tested, which can analyze the Raman spectrum signal and the LIBS spectrum signal cooperatively, and supplement and verify the analysis results, thereby improving the reliability of the detection result and realizing non-invasive early screening of urinary calculus.

[0036] Optionally, the method further comprises the following steps: S01, using a sample with known composition, performing steps S1-S5 to obtain the Raman spectrum signal and the LIBS spectrum signal of the sample; S02, establishing a dual-spectrum signal and urine composition relationship table and a dual-spectrum signal and urinary system stone type relationship table according to the composition of the sample and the obtained Raman spectrum signal and LIBS spectrum signal; S03, replacing the sample with different composition, repeating steps S01-S02 to increase the number of dual-spectrum signal and urinary system stone type relationship controls; S04, summarizing the dual-spectrum signal and urinary system stone type relationship control model data in step S03 to establish a urinary system stone classification model.

[0037] By using the above technical solution, the Raman spectrum signal and the LIBS spectrum signal are obtained using a sample with known composition, the dual-spectrum signal and urine composition relationship table and the dual-spectrum signal and urinary system stone type relationship table are established, the number of controls is increased by replacing the sample with different composition, and the urinary system stone classification model is established by summarizing the control model data.

[0038] Based on the establishment of the urinary system stone classification model, the urinary system stone classification model can provide a reference for subsequent detection of samples with unknown composition, and the composition, whether the sample contains stones, and the type of stones can be determined through the corresponding Raman spectrum signal and LIBS spectrum signal, thereby improving the accuracy and reliability of urinary stone detection.

[0039] In summary, the present application has the following at least one beneficial technical effect:

[0040] 1. The present application obtains the Raman spectrum signal and the LIBS spectrum signal of the sample at the same position, determines the molecular composition information of the sample according to the Raman spectrum signal, determines the elemental composition information of the sample according to the LIBS spectrum signal, and the molecular composition information and the elemental composition information of the sample are verified with each other, which can improve the reliability of the detection result. This detection method realizes the spatio-temporal unification and deep data fusion of molecular information and elemental information, and this cooperative detection method can cross-verify the molecular fingerprint and elemental composition of trace stone crystals from the complex biological background of urine, greatly improving the signal-to-noise ratio and accuracy of detection.

[0041] 2. The present application designs the positions of the microscope objective and the LIBS collection head so that the microscope objective and the LIBS collection head can be focused synchronously. On this basis, through the cooperation of the Z-axis driver, the automatic focusing assembly and the control analysis mechanism, the full-automatic focusing of the microscope objective and the LIBS collection head can be realized, thereby improving the efficiency and accuracy of detection.

[0042] 3. The application can realize plane scanning of the micro objective lens on the sample to be measured through the cooperation of the X-axis driver, the Y-axis driver and the control analysis mechanism. In this process, the control analysis mechanism can acquire the Raman spectrum signal and the LIBS spectrum signal of different positions of the sample to be measured in real time, realize full coverage scanning imaging and omnidirectional detection of the sample to be measured, which can improve the accuracy and comprehensiveness of the detection result. In addition, the surface information of the solid sample in the sample to be measured can be imaged and scanned, and the element and molecular distribution of the solid sample can be detected, which can improve the accuracy of the detection result of the sample to be measured containing the solid sample.

[0043] 4. The application can realize superposition of multiple light paths through the design of the composite light path mechanism, which optimizes the overall light path layout, reduces the number of optical elements, reduces the difficulty of assembly and adjustment of the detection device, and further realizes miniaturization design of the detection device. BRIEF DESCRIPTION OF DRAWINGS

[0044] Figure 1 is the overall structure schematic diagram of the urinary calculus detection device of the embodiment 1 of the application.

[0045] Figure 2 is the overall structure schematic diagram of the Raman excitation light path of the embodiment 1 of the application.

[0046] Figure 3 is the overall structure schematic diagram of the Raman collection light path of the embodiment 1 of the application.

[0047] Figure 4 is the overall structure schematic diagram of the LIBS excitation light path and the LIBS collection light path of the embodiment 1 of the application.

[0048] Figure 5 is the flowchart of the urinary calculus detection method of the embodiment 1 of the application.

[0049] Figure 6 is the flowchart of the urinary system calculus classification model of the embodiment 1 of the application.

[0050] Figure 7 is the flowchart of the establishment of the urinary system calculus classification model of the embodiment 1 of the application.

[0051] Figure 8 is the overall structure schematic diagram of the urinary calculus detection device of the embodiment 1 of the application.

[0052] In the figure, 1, sample platform; 2, microscopic LIBS mechanism; 21, microscope converter; 22, microscope objective; 23, LIBS collection head; 231, double convex lens; 3, composite light path mechanism; 31, wave filter; 32, dichroic mirror; 33, light splitting prism; 4, Raman spectrum generation mechanism; 41, continuous laser; 42, first beam expander; 5, Raman spectrum collection mechanism; 51, first focusing lens; 52, confocal pinhole; 53, second focusing lens; 54, Raman spectrometer; 6, LIBS spectrum generation mechanism; 61, pulsed laser; 62, second beam expander; 63, plane mirror; 7, LIBS spectrum collection mechanism; 71, LIBS spectrometer; 72, collection optical fiber; 8, control analysis mechanism; 81, computer; 82, digital signal generator; 9, scanning imaging mechanism; 91, automatic focusing assembly; 911, CMOS camera; 912, third focusing lens; 92, three-axis moving assembly; 10, polarizer; 100, Raman excitation light path; 200, Raman collection light path; 300, LIBS excitation light path; 400, LIBS collection light path; 500, automatic focusing light path. DETAILED DESCRIPTION

[0053] The following description will be made in conjunction with the accompanying drawings. Figure 1 - The accompanying drawings illustrate the present application. Figure 8 The present application will be further described in detail.

[0054] Example 1: A laser multispectral in-situ integrated urinary calculus detection device, referring to Figure 1 , comprising a sample platform 1, a microscopic LIBS mechanism 2, a composite light path mechanism 3, a Raman spectrum generation mechanism 4, a Raman spectrum collection mechanism 5, a LIBS spectrum generation mechanism 6, a LIBS spectrum collection mechanism 7, and a control analysis mechanism 8. The Raman spectrum generation mechanism 4, the LIBS spectrum generation mechanism 6, the Raman spectrum collection mechanism 5, and the LIBS spectrum collection mechanism 7 are electrically connected with the control analysis mechanism 8. The microscopic LIBS mechanism 2 is arranged opposite to the sample platform 1.

[0055] In this embodiment, the Raman spectrum is a Raman spectrum. The LIBS spectrum is a laser-induced breakdown spectrum.

[0056] Referring to Figure 1 and Figure 2 , the Raman spectrum generation mechanism 4, the composite light path mechanism 3, the microscopic LIBS mechanism 2, and the sample platform 1 are sequentially arranged to form a Raman excitation light path 100.

[0057] Referring to Figure 1 and Figure 3, the Raman spectrum acquisition mechanism 5, the composite light path mechanism 3, the microscopic LIBS mechanism 2 and the sample platform 1 are sequentially arranged to form a Raman acquisition light path 200.

[0058] With reference to Figure 1 and Figure 4 , the LIBS spectrum generation mechanism 6, the composite light path mechanism 3, the microscopic LIBS mechanism 2 and the sample platform 1 are sequentially arranged to form a LIBS excitation light path 300.

[0059] With reference to Figure 1 and Figure 4 , the LIBS spectrum acquisition mechanism 7, the microscopic LIBS mechanism 2 and the sample platform 1 are sequentially arranged to form a LIBS acquisition light path 400.

[0060] Under the cooperation of the Raman excitation light path 100, the Raman acquisition light path 200, the LIBS excitation light path 300 and the LIBS acquisition light path 400, the Raman spectrum system and the LIBS spectrum system can be combined to realize the detection of the components and elements of the urine sample.

[0061] Specifically, with reference to Figure 1 , the urine sample is arranged on the sample platform 1, the Raman spectrum generation mechanism 4 emits continuous laser, and the LIBS spectrum generation mechanism 6 emits pulsed laser. Both the continuous laser and the pulsed laser sequentially pass through the composite light path mechanism 3 and the microscopic LIBS mechanism 2 and then irradiate the sample platform 1, so that the urine sample generates Raman spectrum signals and LIBS spectrum signals. The microscopic LIBS mechanism 2 acquires the Raman spectrum signals and makes the Raman spectrum signals pass through the composite light path mechanism 3 and then enter the Raman spectrum acquisition mechanism 5, so that the Raman spectrum acquisition mechanism 5 acquires the Raman spectrum signals of the urine sample. Similarly, the microscopic LIBS mechanism 2 acquires the LIBS spectrum signals and makes the LIBS spectrum signals enter the LIBS spectrum acquisition mechanism 7, so that the LIBS spectrum acquisition mechanism 7 acquires the LIBS spectrum signals in the urine sample.

[0062] With reference to Figure 1 , on this basis, the control analysis mechanism 8 can acquire the Raman spectrum signals and the LIBS spectrum signals at the same position of the urine sample, and analyze the to-be-detected element information and the molecular information in the urine sample according to the Raman spectrum signals and the LIBS spectrum signals, so as to determine whether the urine sample contains stones, and also to realize the confirmation of the type of the urinary system stones.

[0063] In this embodiment, the continuous laser excites the urine sample to obtain the Raman spectrum signal, and the pulse laser excites the urine sample to obtain the LIBS spectrum signal. The specific parameters of the continuous laser and the pulse laser are known to those skilled in the art and are not described in detail here.

[0064] With reference to Figure 1 The micro-LIBS mechanism 2 includes a microscope converter 21, a microscope objective 22, and a LIBS collection head 23. The microscope objective 22 and the LIBS collection head 23 are both installed on the microscope converter 21. The microscope objective 22 is vertically arranged, the sample platform 1 is located below the microscope objective 22, and the lower end of the microscope objective 22 is arranged opposite to the sample platform 1. The LIBS collection head 23 is located on one side of the microscope objective 22, and a double convex lens 231 is arranged on the LIBS collection head 23. The focal point of the double convex lens 231 coincides with the focal point of the microscope objective 22.

[0065] With reference to Figure 1 The micro-LIBS mechanism 2 integrates the microscope objective 22 and the LIBS collection head 23 on the microscope converter 21, which makes the positions of the microscope objective 22 and the LIBS collection head 23 relatively fixed. The focal point of the double convex lens 231 on the LIBS collection head 23 coincides with the focal point of the microscope objective 22. Therefore, when the microscope objective 22 is focused, after the microscope objective 22 is focused on the urine sample on the sample platform 1, the LIBS collection head 23 is also simultaneously focused on the same position on the urine sample on the sample platform 1. This reduces the focusing difficulty of the micro-LIBS mechanism 2, improves the detection efficiency of the urine sample, and facilitates the acquisition of the LIBS spectrum signal and the Raman spectrum signal of the same position of the sample to be detected.

[0066] In this embodiment, the adjustment method for the coincidence of the focal points of the microscope objective 22 and the LIBS collection head 23 is a known technical solution to those skilled in the art, and is not described in detail here.

[0067] With reference to Figure 2 The Raman spectrum generation mechanism 4 includes a continuous laser 41 and a first beam expander 42. The continuous laser 41, the first beam expander 42, the composite light path mechanism 3, and the microscope objective 22 are sequentially arranged, and the continuous laser emitted by the continuous laser 41 sequentially passes through the first beam expander 42, the composite light path mechanism 3, and the microscope objective 22 and then irradiates onto the sample platform 1.

[0068] When the continuous laser passes through the microscope objective 22, the microscope objective 22 focuses the continuous laser onto the sample platform 1, so that the urine sample generates a Raman spectrum signal.

[0069] In the embodiment, when the continuous laser emitted by the continuous laser 41 passes through the first expansion mirror 42, the first expansion mirror 42 will increase the diameter of the continuous laser and collimate it. The first expansion mirror 42 will expand the diameter of the continuous laser to the optimal receiving range of the objective lens 22, and eliminate the divergence angle of the continuous laser through collimation to ensure the diameter of the focused spot and improve the Raman spectrum signal excitation efficiency.

[0070] With reference to Figure 3 , the Raman spectrum acquisition mechanism 5 includes a first focusing lens 51, a confocal pinhole 52, a second focusing lens 53, and a Raman spectrometer 54. The Raman spectrometer 54, the second focusing lens 53, the confocal pinhole 52, the first focusing lens 51, the composite light path mechanism 3, and the objective lens 22 are sequentially arranged, and the Raman spectrum signal generated by the urine sample sequentially passes through the objective lens 22, the composite light path mechanism 3, the first focusing lens 51, the confocal pinhole 52, and the second focusing lens 53 and then enters the Raman spectrometer 54.

[0071] With reference to Figure 1 and Figure 3 , since the continuous laser emitted by the Raman spectrum generation mechanism 4 causes the urine sample to generate a Raman spectrum signal, the Raman spectrum signal collected by the objective lens 22 is collected by the Raman spectrometer 54 in the Raman spectrum acquisition mechanism 5, which can determine the Raman molecular information in the urine sample according to the Raman spectrum signal.

[0072] In the embodiment, with reference to Figure 3 , after the Raman spectrum signal passes through the second focusing lens 53, the second focusing lens 53 directly couples the Raman spectrum signal into the Raman spectrometer 54 through spatial light, thereby ensuring that the Raman spectrometer 54 can receive the Raman spectrum signal of the urine sample.

[0073] Specifically, with reference to Figure 3 , the first focusing lens 51 and the second focusing lens 53 in the embodiment are aspherical plano-convex lenses provided with a laser anti-reflection film, the wavelength is selected to be 200nm-8000nm, the diameter is selected to be 25.4mm, and the focal length is selected to be 75mm.

[0074] With reference to Figure 1 and Figure 2 , in the embodiment, the composite light path mechanism 3 includes a trap filter 31, and the trap filter 31 is located between the first expansion mirror 42 and the objective lens 22 along the transmission direction of the continuous laser.

[0075] With reference to Figure 1 and Figure 3 , the trap filter 31 is located between the objective lens 22 and the first focusing lens 51 along the transmission direction of the Raman spectrum signal.

[0076] With reference to Figure 2 and Figure 3 , the continuous laser generated by the continuous laser 41 is incident on the wave filter 31 after passing through the first beam expander 42, and the wave filter 31 is incident on the microscope objective 22 after the continuous laser is incident on the wave filter 31. The Raman spectrum signal is incident on the wave filter 31 after passing through the microscope objective 22, and the Raman spectrum signal is incident on the first focusing lens 51 after passing through the wave filter 31.

[0077] The composite light path mechanism 3 is provided with the wave filter 31, so that the Raman excitation light path 100 and the Raman collection light path 200 partially overlap. The wave filter 31 sets a high reflection band for the wavelength of the continuous laser, effectively filters out the Rayleigh scattering noise of the continuous laser, while allowing the Raman spectrum signal to pass through, which ensures the purity of the molecular fingerprint information.

[0078] Specifically, with reference to Figure 2 and Figure 3 , the wave filter 31 in this embodiment is isolated from the scattered light of the continuous laser, and the transmittance outside the bandwidth is 90%, and the diameter is 25.4mm.

[0079] With reference to Figure 1 and Figure 4 , the LIBS spectrum generating mechanism 6 includes a pulsed laser 61, a second beam expander 62 and a plane mirror 63, the pulsed laser 61, the second beam expander 62, the plane mirror 63, the composite light path mechanism 3 and the microscope objective 22 are sequentially arranged, and the pulsed laser emitted by the pulsed laser 61 is incident on the plane mirror 63 after passing through the pulsed laser 61. The plane mirror 63 reflects the pulsed laser to the sample platform 1 after sequentially passing through the composite light path mechanism 3 and the microscope objective 22.

[0080] The LIBS spectrum generating mechanism 6 can generate pulsed laser through the setting of the pulsed laser 61, and under the design of the second beam expander 62, the plane mirror 63 and the composite light path mechanism 3, the pulsed laser can pass through the microscope objective 22 and be focused on the sample platform 1 by the microscope objective 22, so that the plasma is generated on the surface of the urine sample, so that the LIBS spectrum signal can be generated.

[0081] With reference to Figure 4 , in this embodiment, the plane mirror 63 is a flat concave mirror coated with ultraviolet enhancement aluminum film, with a diameter of 25.4mm and a focal length of 150mm. This makes the plane mirror 63 able to accurately deflect the pulsed laser by 90° and reflect it out, maintaining the stability of the LIBS excitation energy. The second beam expander 62 has the same function as the first beam expander 42.

[0082] With reference to Figure 1 andFigure 4 In the embodiment, the composite optical path mechanism 3 further comprises a dichroic mirror 32, the dichroic mirror 32 is arranged in parallel and spaced apart with the plane mirror 63, the pulsed laser 61, the second beam expander 62, the plane mirror 63, the dichroic mirror 32 and the microscope objective 22 are sequentially arranged along the transmission path of the pulsed laser, and the pulsed laser penetrates the dichroic mirror 32 when passing through the dichroic mirror 32.

[0083] Referring to Figure 1 and Figure 2 , the continuous laser 41, the first beam expander 42, the notch filter 31, the dichroic mirror 32 and the microscope objective 22 are sequentially arranged along the transmission path of the continuous laser, and the dichroic mirror 32 reflects the continuous laser when the continuous laser passes through the dichroic mirror 32.

[0084] Referring to Figure 1 and Figure 3 , the Raman spectrometer 54, the second focusing lens 53, the confocal pinhole 52, the first focusing lens 51, the notch filter 31, the dichroic mirror 32 and the microscope objective 22 are sequentially arranged along the transmission path of the Raman spectrum signal, and the dichroic mirror 32 reflects the Raman spectrum signal when the Raman spectrum signal passes through the dichroic mirror 32.

[0085] Therefore, the composite optical path mechanism 3 makes the Raman excitation light path 100, the Raman collection light path 200 and the LIBS excitation light path 300 partially overlap through the design of the dichroic mirror 32.

[0086] Referring to Figure 1 and Figure 4 , the dichroic mirror 32 has high reflectivity to the continuous laser and high transmissivity to the pulsed laser. On this basis, the dichroic mirror 32 is designed to be inclined at 45°, which makes the continuous laser and the pulsed laser coaxially fuse after passing through the dichroic mirror 32, so that the continuous laser and the pulsed laser can be focused to the same point of the urine sample after passing through the microscope objective 22, thereby generating the Raman spectrum signal and the LIBS spectrum signal at the same position of the urine sample.

[0087] Specifically, the dichroic mirror 32 in the embodiment adopts a long-wave dichroic mirror, which has high reflectivity in the short-wave region and high transmissivity in the long-wave region, and is also used to reflect stray light of incident laser.

[0088] Referring to Figure 1 and Figure 4 , the LIBS spectrum collection mechanism 7 comprises a LIBS spectrometer 71 and a collection optical fiber 72, one end of the collection optical fiber 72 is connected with the LIBS collection head 23, and the other end is connected with the LIBS spectrometer 71.

[0089] Based on the cooperation design of the LIBS spectrometer 71 and the collection optical fiber 72, the urine sample generates a LIBS spectrum signal due to the pulsed laser emitted by the LIBS spectrum generating mechanism 6, the LIBS collection head 23 can collect the LIBS spectrum signal through the built-in double convex lens 231, and the LIBS spectrum signal is transmitted into the collection optical fiber 72, and the LIBS spectrum signal is coupled into the LIBS spectrometer 71 through the collection optical fiber 72, so that the LIBS spectrum signal collection can be realized, and then the LIBS spectrum element information in the urine sample can be determined according to the LIBS spectrum signal.

[0090] Specifically, in the embodiment, the incident end face of the collection optical fiber 72 is composed of 19 200 μm optical fibers arranged in a circular shape, and the exit end face is a long strip shape arranged in 1x19, which can effectively enhance the signal strength collected by the collection optical fiber 72.

[0091] Referring to Figure 1 and Figure 5 , the urinary calculus detection device further comprises a scanning imaging mechanism 9, the scanning imaging mechanism 9 comprises an automatic focusing assembly 91 and a three-axis moving assembly 92, the sample platform 1 is arranged on the three-axis moving assembly 92, and the automatic focusing assembly 91 and the three-axis moving assembly 92 are electrically connected with the control analysis mechanism 8. The automatic focusing assembly 91, the composite light path mechanism 3, the microscope objective 22 and the sample platform 1 are sequentially arranged to form an automatic focusing light path 500.

[0092] Based on the formation of the automatic focusing light path 500, the Raman spectrum signal enters the automatic focusing assembly 91 after sequentially passing through the microscope objective 22 and the composite light path mechanism 3, and the Raman spectrum signal is automatically imaged after entering the automatic focusing assembly 91. The control analysis mechanism 8 performs image processing, and controls the three-axis moving assembly 92 according to the image processing result, so as to realize the adjustment of the position of the sample platform 1, thereby realizing the automatic focusing of the microscope objective 22 and the LIBS collection head 23.

[0093] Referring to Figure 5 , in the embodiment, the three-axis moving assembly 92 comprises an X-axis driver, a Y-axis driver and a Z-axis driver, the X-axis driver, the Y-axis driver and the Z-axis driver are sequentially connected, the sample platform 1 is arranged on the Z-axis driver, the driving direction of the Z-axis driver is arranged along the vertical direction, the driving directions of the X-axis driver, the Y-axis driver and the Z-axis driver are arranged perpendicular to each other, and the X-axis driver, the Y-axis driver and the Z-axis driver are electrically connected with the control analysis mechanism 8.

[0094] The triaxial moving assembly 92 realizes accurate regulation of the sample platform 1 through the cooperative action of the X-axis driver, the Y-axis driver and the Z-axis driver. The Z-axis driver drives the sample platform 1 to move in the vertical direction, so as to adjust the distance between the sample platform 1 and the microscope objective 22, thereby facilitating the auto-focusing of the microscope objective 22 and the LIBS collection head 23. The cooperation of the X-axis driver and the Y-axis driver enables the microscope objective 22 and the LIBS collection head 23 to perform planar scanning on the sample platform 1, thereby realizing multi-point detection of the urine sample.

[0095] Specifically, in the embodiment, the Z-axis driver adopts a piezoelectric ceramic actuator or a linear motor driver, and the X-axis driver and the Y-axis driver both adopt linear motor drivers.

[0096] With reference to Figure 1 and Figure 5 In the embodiment, the auto-focusing assembly 91 comprises a CMOS camera 911 and a third focusing lens 912. The CMOS camera 911 is electrically connected to the control analysis mechanism 8. The CMOS camera 911, the third focusing lens 912, the composite light path mechanism 3 and the microscope objective 22 are sequentially arranged, and the Raman spectrum signal enters the CMOS camera 911 after sequentially passing through the microscope objective 22, the composite light path mechanism 3 and the third focusing lens 912.

[0097] Therefore, the CMOS camera 911 can acquire part of the Raman spectrum signal for imaging and transmit the image information to the control analysis mechanism 8.

[0098] With reference to Figure 1 and Figure 5 In the embodiment, the composite light path mechanism 3 further comprises a light splitting prism 33. The CMOS camera 911, the third focusing lens 912, the light splitting prism 33, the dichroic mirror 32 and the microscope objective 22 are sequentially arranged. The Raman spectrum signal is reflected to the light splitting prism 33 after sequentially passing through the microscope objective 22 and the dichroic mirror 32. The light splitting prism 33 splits the Raman spectrum signal. Part of the Raman spectrum signal is split and reflected to the third focusing lens 912 after passing through the light splitting prism 33, and finally enters the CMOS camera 911 for imaging.

[0099] With reference to Figure 3 and Figure 5 When the Raman spectrum signal passes through the light splitting prism 33, the light splitting prism 33 splits the Raman spectrum signal. Part of the Raman spectrum signal is split and reflected to the third focusing lens 912 after passing through the light splitting prism 33, and another part of the Raman spectrum signal penetrates the light splitting prism 33 and finally enters the Raman spectrometer 54.

[0100] Therefore, the composite optical path mechanism 3 partially overlaps the Raman collection optical path 200 and the autofocus optical path 500 through the design of the light splitting prism 33, so that part of the Raman spectrum signal can be transmitted into the Raman spectrometer 54, thereby realizing the acquisition of the Raman spectrum signal, and part of the Raman spectrum signal is introduced into the CMOS camera 911 for imaging, which can trigger the function of the autofocus assembly 91, and then realize the autofocus function of the microscope objective 22.

[0101] Specifically, referring to Figure 1 and Figure 5 , the transmission and reflection ratios of the light splitting prism 33 in the embodiment are 5:5.

[0102] Referring to Figure 1 and Figure 2 , the notch filter 31, the light splitting prism 33 and the dichroic mirror 32 are arranged in sequence along the transmission path of the continuous laser, and the continuous laser passes through the notch filter 31, the light splitting prism 33 and the dichroic mirror 32 in sequence, and the continuous laser passes through the light splitting prism 33.

[0103] Referring to Figure 1 and Figure 3 , the Raman spectrum signal passes through the dichroic mirror 32, the light splitting prism 33 and the notch filter 31 in sequence, and part of the Raman spectrum signal penetrates the light splitting prism 33 when the Raman spectrum signal passes through the light splitting prism 33.

[0104] Referring to Figure 1 and Figure 5 , part of the Raman spectrum signal penetrates the light splitting prism 33 when the Raman spectrum signal passes through the light splitting prism 33.

[0105] Therefore, the composite optical path mechanism 3 partially overlaps the Raman collection optical path 200, the autofocus optical path 500 and the Raman excitation optical path 100 through the design of the light splitting prism 33.

[0106] Referring to Figure 1 , in the embodiment, the composite optical path mechanism 3 realizes the overlapping and sharing of multiple optical paths through the cooperative design of the notch filter 31, the light splitting prism 33 and the dichroic mirror 32, which can optimize the overall optical path layout inside the urinary stone detection device, and also realizes the multifunctional reuse of a single element, thereby reducing the number of optical elements, reducing the assembly and adjustment difficulty of the urinary stone detection device, and fundamentally compressing the physical space of the device, realizing the miniaturization and compactness of the device. Therefore, the complex dual-spectrum system is integrated on a simple and efficient optical platform, effectively solving the defects of complex optical path structure and large volume in the prior art.

[0107] Referring to Figure 1The control analysis mechanism 8 comprises a computer 81 and a digital signal generator 82, and the computer 81 is electrically connected with the digital signal generator 82.

[0108] Referring to FIG. 1 and Figure 2 The continuous laser 41 is electrically connected with the digital signal generator 82, so that the digital signal generator 82 can be parameterized by the computer 81 to drive the continuous laser 41 to work, so that the continuous laser 41 generates continuous laser.

[0109] Similarly, referring to FIG. 1 and Figure 1 and Figure 4 The pulsed laser 61 is electrically connected with the digital signal generator 82, so that the pulsed laser 61 generates pulsed laser.

[0110] In the embodiment, the digital signal generator 82 is a DG645 digital delay pulse transmitter, which is used to control the time delay between the pulsed laser and the continuous laser to realize the time-sharing excitation function. The continuous laser 41 is a single longitudinal mode continuous laser 41, and the pulsed laser 61 is a Nd:YAG nanosecond pulse Q-switched laser.

[0111] Similarly, referring to FIG. 1 and Figure 1 and Figure 3 The Raman spectrometer 54 is electrically connected with the computer 81, so that the computer 81 can read and analyze the Raman spectrum signal.

[0112] Similarly, referring to FIG. 1 and Figure 1 and Figure 4 The LIBS spectrometer 71 is electrically connected with the computer 81, so that the computer 81 can read and analyze the LIBS spectrum signal.

[0113] Referring to FIG. 1 and Figure 1 and Figure 5 The CMOS camera 911, the X-axis driver, the Y-axis driver and the Z-axis driver are electrically connected with the computer 81, the computer 81 can obtain the image information generated in the CMOS camera 911, the computer 81 can process the image information, and according to the processing result, the Z-axis driver is adjusted, so that the automatic focusing function is realized. At the same time, the computer 81 can also drive the X-axis driver and the Y-axis driver to realize the sample platform position adjustment, so that the microscopic objective 22 and the LIBS collection head 23 can be focused to different positions of the urine sample, so that the multi-point collection of the urine sample is realized.

[0114] On this basis, the X-axis driver and the Y-axis driver cooperate to drive the sample platform 1 to move, which enables the microscopic objective 22 and the LIBS collection head 23 to scan the urine sample on the sample platform 1. In this process, the computer 81 can acquire the Raman spectrum signal and the LIBS spectrum signal of the urine sample at different positions in real time, which can realize full-coverage scanning imaging of the urine sample and achieve omnidirectional detection of the urine sample. In particular, when there is a solid sample in the urine sample, for example, there is a combined crystal, the urinary stone detection device can image and scan the surface information of the solid sample and detect the distribution of elements and molecules, so the urinary stone detection device can realize detection of the urine sample and composition analysis of the solid sample.

[0115] The implementation principle of the embodiment of the present application is that, under the cooperation of the Raman spectrum generating mechanism 4, the Raman spectrum acquisition mechanism 5, the composite light path mechanism 3, the microscopic objective 22 and the sample platform 1, the continuous laser emitted by the Raman spectrum generating mechanism 4 irradiates the to-be-detected sample on the sample platform 1 in sequence through the composite light path mechanism 3 and the microscopic objective 22, and makes the to-be-detected sample generate a Raman spectrum signal, and the Raman spectrum acquisition mechanism 5 can acquire the Raman spectrum signal of the to-be-detected sample, and the molecular composition information of the to-be-detected sample can be acquired according to the Raman spectrum signal of the to-be-detected sample.

[0116] Under the cooperation of the LIBS spectrum generating mechanism 6, the LIBS spectrum acquisition mechanism 7, the composite light path mechanism 3, the microscopic objective 22, the sample platform 1 and the LIBS collection head 23, the pulsed laser emitted by the LIBS spectrum generating mechanism 6 irradiates the to-be-detected sample on the sample platform 1 in sequence through the composite light path mechanism 3 and the microscopic objective 22, so as to laser-ablate the to-be-detected sample, make the to-be-detected sample generate plasma, and then the plasma generates a LIBS spectrum signal. The LIBS spectrum acquisition mechanism 7 and the LIBS collection head 23 can cooperate to acquire the LIBS spectrum signal of the to-be-detected sample, and the element composition information of the to-be-detected sample can be acquired according to the LIBS spectrum signal of the to-be-detected sample.

[0117] On this basis, since the Raman spectrum signal and the LIBS spectrum signal are acquired at the same position of the to-be-detected sample, the Raman spectrum signal and the LIBS spectrum signal can be verified with each other, so that the acquired molecular composition information and element composition information of the to-be-detected sample are complementary and verified with each other, which can realize comprehensive collaborative detection of the element and molecular composition of the urinary stone, which can determine whether the patient has a stone through the urine sample of the patient, and can also detect the type of urinary system stone of the patient, which can realize non-invasive early screening of urinary stones.

[0118] The embodiment also discloses a laser multispectral in-situ integrated urinary stone detection method, referring to Figure 6 , comprising the following steps:

[0119] S1, sample preparation: collecting a urine sample and preparing a to-be-tested sample, and placing the to-be-tested sample on a sample platform 1.

[0120] Specifically, a urine sample of a stone patient is collected, 10 uL of the urine sample is dropped on a glass slide to prepare a to-be-tested sample, and then the prepared to-be-tested sample is loaded onto the sample platform 1.

[0121] S2, focusing: focusing the microscopic objective 22 and the LIBS collection head 23 on the to-be-tested sample.

[0122] Specifically, in the embodiment, the microscopic objective 22 and the LIBS collection head 23 are automatically focused on the to-be-tested sample, and the automatic focusing step is as follows:

[0123] S21, the Raman spectrum generating mechanism 4 emits continuous laser, and the continuous laser is sequentially irradiated to the to-be-tested sample on the sample platform 1 after passing through the wave filter 31, the light splitting prism 33, the dichroic mirror 32 and the microscopic objective 22, the continuous laser excites the to-be-tested sample and generates a Raman spectrum signal.

[0124] S22, the Raman spectrum signal is sequentially irradiated to the light splitting prism 33 after passing through the microscopic objective 22 and the dichroic mirror 32, at this time, the light splitting prism 33 reflects part of the Raman spectrum signal to pass through the third focusing lens 912 and finally enters the CMOS camera 911.

[0125] S23, the CMOS camera 911 images the part of the Raman spectrum signal, so as to realize image acquisition of the to-be-tested sample, and transmits the acquired image information to the computer 81, and the computer 81 processes and analyzes the image.

[0126] S24, the computer 81 drives the Z-axis driver according to the image processing result, so that the sample platform 1 moves in the vertical direction.

[0127] Specifically, a gradient-based image sharpness evaluation algorithm is preferably adopted, for example, a Tenengrad function. The computer 81 calculates the Tenengrad value of the image in real time, and controls the Z-axis driver to scan in a preset range to find the Z-axis position at which the Tenengrad function value reaches the maximum, which is the best focal plane.

[0128] S25, repeat the above steps S21-S24 until the computer 81 no longer drives the Z-axis driver in step S24, at this time, the microscopic objective 22 and the LIBS collection head 23 are focused on the to-be-tested sample.

[0129] S3, Raman spectrum signal excitation and collection: the Raman spectrum generating mechanism 4 emits continuous laser, the continuous laser sequentially passes through the composite light path mechanism 3 and the microscope objective 22 and then irradiates on the sample to be measured, and the sample to be measured generates Raman spectrum signal; the Raman spectrum signal sequentially passes through the microscope objective 22 and the composite light path mechanism 3 and then enters the Raman spectrum collection mechanism 5.

[0130] Specifically, when the Raman spectrum signal is generated, the continuous laser 41 generates continuous laser, and the continuous laser sequentially passes through the first expansion mirror 42, the notch filter 31, the beam splitter prism 33, the dichroic mirror 32, enters the microscope objective 22 and is finally focused on the surface of the sample to be measured. When the Raman spectrum signal is collected, the Raman spectrum signal sequentially passes through the microscope objective 22, the dichroic mirror 32, the beam splitter prism 33, the notch filter 31, the first focusing lens 51, the confocal pinhole 52, the second focusing lens 53 and enters the Raman spectrometer 54.

[0131] In this process, when the Raman spectrum signal passes through the beam splitter prism 33, part of the Raman spectrum signal passes through the beam splitter prism 33, and part of the Raman spectrum signal is reflected to the CMOS camera 911 by the beam splitter prism 33.

[0132] S4, LIBS spectrum signal excitation and collection: the LIBS spectrum generating mechanism 6 emits pulsed laser, the pulsed laser sequentially passes through the composite light path mechanism 3 and the microscope objective 22 and then irradiates on the sample to be measured, and the sample to be measured generates LIBS spectrum signal; the LIBS collection head 23 collects the LIBS spectrum signal and inputs it into the LIBS spectrum collection mechanism 7.

[0133] Specifically, when the LIBS spectrum signal is generated, the pulsed laser 61 generates pulsed laser, and the pulsed laser sequentially passes through the second expansion mirror 62, the plane mirror 63, the dichroic mirror 32 and the microscope objective 22 and is focused on the surface of the sample to be measured. When the LIBS spectrum signal is collected, the LIBS spectrum signal is focused after passing through the double-convex lens 231 and then enters the core end face of the collection optical fiber 72, and then is transmitted to the LIBS spectrometer 71 through the collection optical fiber 72.

[0134] In this embodiment, the Raman spectrum signal obtained in step S3 and the LIBS spectrum signal obtained in step S4 come from the same position of the sample to be measured, which enables the Raman spectrum signal and the LIBS spectrum signal to be analyzed in coordination with each other.

[0135] S5, multi-point data spectrum signal collection: adjust the position of the sample platform 1, and repeat the above steps S3-S4 to obtain several groups of Raman spectrum signals and LIBS spectrum signals.

[0136] Specifically, the computer 81 drives the X-axis driver and the Y-axis driver to change the position of the sample platform 1, so as to focus the objective lens and the LIBS collection head 23 to different positions of the sample to be detected. In this case, repeating the steps S3-S4 can realize the detection function of different positions of the sample to be detected.

[0137] Further, when the number and density of the detection positions of the sample to be detected are continuously increased, the microscopic objective lens 22 and the LIBS collection head 23 can perform plane scanning on the sample to be detected. In this process, the computer 81 can acquire the Raman spectrum signal and the LIBS spectrum signal of different positions of the sample to be detected in real time, which can realize full-coverage scanning imaging of the sample to be detected and realize omnidirectional detection of the sample to be detected. When there is a solid sample in the sample to be detected, for example, there is a combined crystal, the urinary stone detection device can image and scan the surface information of the solid sample and detect the distribution of elements and molecules. Therefore, the urinary stone detection device can realize both detection of the sample to be detected and analysis of the solid sample.

[0138] S6, spectrum signal analysis: the control analysis mechanism 8 acquires a plurality of groups of Raman spectrum signals and LIBS spectrum signals, and analyzes the composition of the sample to be detected according to the plurality of groups of Raman spectrum signals and LIBS spectrum signals.

[0139] Specifically, the computer 81 acquires the Raman spectrum signal and the LIBS spectrum signal of different positions of the sample to be detected, and determines the molecular information of the corresponding position of the sample to be detected according to the Raman spectrum signal and determines the element information of the corresponding position of the sample to be detected according to the LIBS spectrum signal. Therefore, under the cooperation of a plurality of groups of Raman spectrum signals and LIBS spectrum signals, the composition of the sample to be detected can be quickly determined.

[0140] Specifically, after the computer 81 acquires a plurality of groups of Raman spectrum signals and LIBS spectrum signals, first, the original spectrum data is preprocessed, including baseline correction by polynomial fitting or asymmetric least squares method, and noise filtering by Savitzky-Golay smoothing algorithm. Second, the preprocessed Raman spectrum is compared with the preconfigured standard Raman spectrum library of stone composition to preliminarily identify the molecular species by using the correlation coefficient method or the peak matching method; for the LIBS spectrum, the NIST atomic spectrum database is queried to identify the characteristic spectral lines of elements such as Ca, Mg, P, C, N related to the stone. Finally, the molecular information identified by the Raman spectrum and the element information identified by the LIBS spectrum are jointly judged, for example, when the Raman spectrum identifies the characteristic peak of calcium oxalate and the LIBS spectrum detects a significant Ca element signal, it is confirmed that the calcium oxalate composition is cooperated, so as to complete the composition analysis of the sample to be detected.

[0141] S7, detection result output: determining whether the sample contains stones or the type of stones according to the composition of the sample to be detected.

[0142] Specifically, after determining the composition of the sample to be detected, it is determined whether the sample to be detected contains stones according to the pre-established urinary stone classification model, and the type of stones in the sample to be detected is also determined.

[0143] In order to establish the urinary stone classification model, refer to Figure 7 , the following steps are also included:

[0144] S01, using the sample to be detected with a clear composition, performing the above steps S1-S5 to obtain the Raman spectrum signal and the LIBS spectrum signal of the sample to be detected.

[0145] S02, according to the composition of the sample to be detected in step S01 and the obtained Raman spectrum signal and LIBS spectrum signal, a dual-spectrum signal and urine composition relationship table is established, and a dual-spectrum signal and urinary stone type relationship table is also established.

[0146] S03, replace the sample to be detected with different composition, repeat the above steps S01-S02, increase the number of dual-spectrum signal and urinary stone type relationship table.

[0147] S04, summarize the dual-spectrum signal and urinary stone type relationship table data in the above step S03 to establish a urinary stone classification model.

[0148] Specifically, in step S04, after summarizing the dual-spectrum signal and urinary stone type relationship table data in step S03, a machine learning algorithm such as support vector machine or random forest is used to establish a urinary stone classification model. The input features of the model are the feature vectors of the preprocessed Raman spectrum data and LIBS spectrum data, and the output is the classification of stones. The model is trained and evaluated by cross-validation method until the classification accuracy, sensitivity and specificity of the model reach the preset clinical diagnosis requirements.

[0149] In this embodiment, after steps S01-S04, a urinary stone classification model can be established. Therefore, when detecting a sample to be detected with an unclear composition, the Raman spectrum signal and the LIBS spectrum signal of the sample to be detected can be determined after the above steps S1-S7, and at this time, the composition of the sample to be detected, whether it contains stones and the type of stones can be determined according to the urinary stone classification model and the corresponding Raman spectrum signal and LIBS spectrum signal.

[0150] The implementation principle of the embodiment of the present application is that the above detection method can realize the function of automatic focusing after loading the sample to be detected, and can further realize automatic acquisition of Raman spectrum signals and LIBS spectrum signals and automatic determination of whether the sample to be detected contains stones or the types of stones, which can realize full-automatic detection of the sample to be detected, thereby improving the detection efficiency. Since the above detection method can acquire the Raman spectrum signals and the LIBS spectrum signals generated at the same position of the sample to be detected, the Raman spectrum signals and the LIBS spectrum signals can be analyzed cooperatively, and the analysis results can be mutually supplemented and verified, thereby improving the reliability of the detection results.

[0151] Embodiment 2: A laser multispectral in-situ integrated urinary stone detection device, referring to Figure 8 The difference between the present embodiment and embodiment 1 is that the polarizer 10 is arranged on the Raman excitation light path 100, the Raman collection light path 200 and the LIBS excitation light path 300.

[0152] Specifically, the polarizer 10 on the Raman excitation light path 100 is located between the first beam expander 42 and the wave filter 31, and the continuous laser passes through the first beam expander 42 and the corresponding polarizer 10 in turn and then irradiates on the wave filter 31. The polarizer 10 of the Raman collection light path 200 is located between the wave filter 31 and the first focusing lens 51, and the Raman signal passes through the wave filter 31, the corresponding polarizer 10 and the first focusing lens 51 in turn. The polarizer 10 of the LIBS excitation light path 300 is located between the plane mirror 63 and the dichroic mirror 32, and the pulsed laser is reflected by the plane mirror 63 and then passes through the corresponding polarizer 10 and the corresponding dichroic mirror 32 once.

[0153] The implementation principle of the embodiment of the present application is that the polarizer 10 is arranged on the Raman excitation light path 100, the Raman collection light path 200 and the LIBS excitation light path 300, so that the continuous laser, the pulsed laser and the Raman spectrum signal all pass through the corresponding polarizer 10. The polarizer 10 can control and adjust the polarization state of the corresponding continuous laser, pulsed laser and Raman spectrum signal, reduce stray light interference, improve the purity and quality of the continuous laser, pulsed laser and Raman spectrum signal, and further improve the accuracy and reliability of the detection results of the urinary stone detection device.

[0154] The embodiments of the present application are preferred embodiments of the present application, and are not limited to the protection scope of the present application. The same parts are indicated by the same reference numerals. Therefore, equivalent changes made according to the structure, shape and principle of the present application should be covered by the protection scope of the present application.

Claims

1. A laser-based multispectral in-situ integrated urinary stone detection device, characterized in that, The utility model relates to a kind of micro-LIBS platform, including: Sample platform (1);Micro-LIBS mechanism (2), including micro-objective (22) and LIBS collection head (23), the micro-objective (22) and the LIBS collection head (23) are all towards the sample platform (1);Composite light path mechanism (3);Raman spectrum generation mechanism (4) is used to generate continuous laser;Continuous laser is irradiated to the sample platform (1) after sequentially passing through the composite light path mechanism (3) and the micro-objective (22), for generating Raman spectrum signal;Raman spectrum acquisition mechanism (5), Raman spectrum signal enters the Raman spectrum acquisition mechanism (5) after sequentially passing through the micro-objective (22) and the composite light path mechanism (3);LIBS spectrum generation mechanism (6) is used to generate pulsed laser, pulsed laser is irradiated to the sample platform (1) after sequentially passing through the composite light path mechanism (3) and the micro-objective (22), for generating LIBS spectrum signal;LIBS spectrum acquisition mechanism (7), the LIBS collection head (23) is used to collect LIBS spectrum signal and input the LIBS spectrum acquisition mechanism (7);Still including control analysis mechanism (8), the Raman spectrum generation mechanism (4), LIBS spectrum generation mechanism (6), Raman spectrum acquisition mechanism (5) and LIBS spectrum acquisition mechanism (7) are electrically connected with the control analysis mechanism (8); The micro-LIBS mechanism (2) further includes microscope converter (21), the micro-objective (22) and the LIBS collection head (23) are both arranged on the microscope converter (21), and the focus point of the LIBS collection head (23) coincides with the focus point position of the micro-objective (22).

2. The device according to claim 1, wherein the device is a laser-based multispectral in-situ integrated urinary stone detection device. The micro-objective (22) is vertically arranged, the sample platform (1) is arranged below the micro-objective (22), and the lower end of the micro-objective (22) is arranged opposite to the sample platform (1);A Z-axis driver is arranged on the sample platform (1), the Z-axis driver is used to drive the sample platform (1) to move in vertical direction, and the Z-axis driver is electrically connected with the control analysis mechanism (8).

3. The device according to claim 2, wherein the device is a laser-based multispectral in-situ integrated urinary stone detection device. Further including X-axis driver and Y-axis driver, the X-axis driver, Y-axis driver and Z-axis driver are sequentially connected, and the driving direction of the X-axis driver and the Y-axis driver is arranged perpendicular to each other, the driving direction of the X-axis driver and the Y-axis driver is arranged perpendicular to the driving direction of the Z-axis driver, and the X-axis driver and the Y-axis driver are electrically connected with the control analysis mechanism (8).

4. The device according to claim 2, wherein the device is a laser-based multispectral in-situ integrated urinary stone detection device. Further comprising an auto-focusing assembly (91), the auto-focusing assembly (91), the composite light path mechanism (3) and the microscope objective (22) are sequentially arranged; the Raman spectrum signal enters the auto-focusing assembly (91) and the Raman spectrum acquisition mechanism (5) after sequentially passing through the microscope objective (22) and the composite light path mechanism (3); the auto-focusing assembly (91) is electrically connected with the control analysis mechanism (8).

5. The device according to claim 1, wherein the device is a laser-based multispectral in-situ integrated urinary stone detection device. The composite light path mechanism (3) comprises a trap filter (31); the Raman spectrum generating mechanism (4), the trap filter (31) and the microscope objective (22) are sequentially arranged along the transmission direction of continuous laser, the trap filter (31) reflects continuous laser; the Raman spectrum acquisition mechanism (5), the trap filter (31) and the microscope objective (22) are sequentially arranged along the transmission direction of Raman spectrum signal, the Raman spectrum signal passes through the trap filter (31).

6. The device according to claim 1, wherein the device is a laser-based multispectral in-situ integrated urinary stone detection device. The composite light path mechanism (3) comprises a dichroic mirror (32); the Raman spectrum generating mechanism (4), the dichroic mirror (32) and the microscope objective (22) are sequentially arranged along the transmission direction of continuous laser, the dichroic mirror (32) reflects continuous laser; the LIBS spectrum generating mechanism (6), the dichroic mirror (32) and the microscope objective (22) are sequentially arranged along the transmission direction of pulsed laser, the pulsed laser passes through the dichroic mirror (32).

7. The device according to claim 1, wherein the device is a laser-based multispectral in-situ integrated urinary stone detection device. The Raman spectrum generating mechanism (4), the Raman spectrum acquisition mechanism (5) and the LIBS spectrum generating mechanism (6) are provided with a polaroid (10) between the composite light path mechanism (3), the continuous laser, the pulsed laser and the Raman spectrum signal all pass through the corresponding polaroid (10).

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