Auxiliary device for bilateral common carotid artery operation of experimental animal

Abstract

The invention discloses an auxiliary device for bilateral common carotid artery surgery of an experimental animal, and belongs to the technical field of animal experimental equipment. Each traction plate is provided with a closed end part and a traction end part which are deviated from each other, each closed end part is provided with a connecting piece, and when the two traction plates are connected through the connecting pieces, a clamping and closing area is formed between the opposite closed end parts; the traction end part is provided with a traction hook; the two tail ends of the traction rope are connected with the pad body and the traction plate respectively, and when the traction plate is connected with a wound of the neck of the mouse in a hooked mode, the traction rope pulls the edge of the wound in the opposite direction so that the wound can be continuously opened, and carotid arteries on the two sides can be exposed; when the closed ends move oppositely and clamp the edges of the wounds on the two sides, the connecting pieces are connected with each other, and at the moment, the connecting pieces overcome driving of the traction rope to the traction plate and clamp the wounds in a clamping area. By means of the arrangement, a wound can be opened or clamped in two stages of the operation process.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of animal experiment equipment, and particularly relates to an experimental animal bilateral common carotid artery operation auxiliary device. BACKGROUND

[0002] In neuroscience and cerebrovascular disease research, bilateral common carotid artery stenosis surgery is a common means to establish a chronic hypoperfusion model. The surgery needs to expose the bilateral common carotid artery under anesthesia and perform fine operation. The researcher performs fine operation on the bilateral common carotid artery of the mouse twice, which takes about 45 minutes. The operation time interval of the bilateral common carotid artery is 30 minutes. Since excessive anesthetic drugs can cause multi-system physiological disorders and seriously affect the operation and postoperative recovery, the anesthesia is performed twice. The interval between the two single operations is just enough for the mouse to wake up slightly, so as to judge the state of the mouse and ensure that the next operation can be performed on it.

[0003] During the operation of the mouse, the abdomen is upward, but during the wake-up stage between the two single operations, the mouse with blurred consciousness may turn over and make the wound downward when moving the limbs, so there is a risk of wound contamination and tissue damage in the wound.

[0004] Therefore, a device is designed to open or close the wound in two stages of the operation, which not only meets the operation habit of the researcher when performing common carotid artery stenosis surgery, but also reduces the risk of wound contamination or tissue damage of the mouse during the interval between the bilateral vascular long-time operation. The device is specifically an experimental animal bilateral common carotid artery operation auxiliary device. SUMMARY

[0005] In order to overcome the problems in the background art, the present application adopts the following technical scheme:

[0006] An experimental animal bilateral common carotid artery operation auxiliary device, comprising: a pad; a traction plate having a closed end and a traction end which are away from each other, the closed end is provided with a connecting piece, when two traction plates are connected through the connecting piece, the opposite closed ends form a clamping area; the traction end is provided with a traction hook; a traction rope, two ends of which are connected to the pad and the traction plate respectively, when the traction hook is hooked to the wound of the mouse neck, the traction rope pulls the wound edge in the opposite direction to make the wound continuously open and expose the bilateral carotid artery; when the closed ends move towards each other and clamp the wound edges on both sides, the connecting pieces are connected to each other, at this time, the connecting pieces overcome the driving of the traction rope on the traction plate and clamp the wound in the clamping area.

[0007] Further, the closed end is further connected with a limiting protrusion, when the two traction plates are connected through the connecting piece, the clamping area is between the limiting protrusion and the connecting piece, at this time, the distance between the two limiting protrusions is smaller than the distance between the two closed ends, and the distance between the two limiting protrusions is not greater than 300 μm.

[0008] Further, the pad body has a cavity in the inside, an electric heating element is connected in the cavity, one end of the electric heating element is further connected with a lead wire, the other end of the lead wire is exposed outside through the pad body and is connected with a connector, when the connector is connected with a power supply, the electric heating element works and heats up.

[0009] Further, the pad body has a surgical end, the surgical end is connected with a limiting assembly, the limiting assembly includes a limiting rope and a limiting buckle, one end of the limiting rope is connected with the surgical end, the other end of the limiting rope passes through the limiting buckle twice and forms a constraint ring, and the limiting rope changes the circumference of the constraint ring when the limiting rope and the limiting buckle move relative to each other. The number of limiting assemblies is not less than four, and when the number of limiting assemblies is four, the four limbs of a mouse after anesthesia can be fixed respectively, and by controlling the length of the limiting rope passing through the limiting buckle for the second time, the size of the constraint ring can be controlled, so that the four limbs of the experimental mouse are better adapted.

[0010] Further, the limiting buckle includes a shell and an elastic element, at least two through holes are provided in the shell, the elastic element is connected with the inner wall of the shell, and when the elastic element is not subjected to external force, part of the elastic element protrudes from the shell and blocks the through holes. When the elastic element is deformed into an energy storage state under the driving of external force, the through holes are restored to be in communication.

[0011] Further, when the elastic element extends outward from the energy storage state, the limiting rope passing through the through hole is pressed against the inner wall of the shell, and when the elastic element is deformed under force to the energy storage state, the traction rope can move relative to the through hole. When the elastic element is not subjected to external force, the surrounding circumference of the constraint ring formed by the limiting rope is constant, the fastening degree of the limbs of the mouse surrounded by the constraint ring is unchanged, and the mouse can be effectively prevented from sliding off the surgical end of the pad body when the pad body is placed on an inclined plane with an angle.

[0012] Further, the traction rope is connected with the surgical end.

[0013] Further, the number of limiting buckles in each limiting assembly is at least one. When the number of limiting buckles exceeds one, the limiting buckles can play the role of collecting the limiting ropes, and the limiting effect on the limiting ropes is enhanced.

[0014] Further, the pad body further has a contact end, the contact end is away from the surgical end, the contact end faces a plane in contact with the pad body when working, and the contact end is connected with a plurality of suction cups. The plurality of suction cups can stably adsorb the pad body on various planes.

[0015] Further, the width of the limiting protrusion is greater than the width of the traction hook.

[0016] Advantages of the present application:

[0017] The present application is symmetrically provided with traction plates, and the two ends of the traction plates, which are away from each other, are respectively connected with traction hooks, so that the wounds on the necks of mice can be pulled apart to the opposite sides to expose the surgical field; the opposite closed ends of the traction plates are further provided with connecting members, and when the two traction plates are connected through the connecting members, the edges of the wounds can be clamped to be closed, so that the inside and outside of the wounds are temporarily isolated in space during the interval of two operations, preventing the wounds from being pulled by the mice in a state of blurred consciousness, or the blood vessels or tissues in the wounds from being damaged when the mice turn over and crawl. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor. Among them:

[0019] Figure 1 It is one of the overall structure schematic diagrams of the present application when the traction plates are not connected;

[0020] Figure 2 It is one of the overall structure schematic diagrams of the present application when the traction plates are not connected; Figure 1 It is one of the cross-sectional structure schematic diagrams of the present application;

[0021] Figure 3 It is one of the cross-sectional structure schematic diagrams of the present application; Figure 2 It is one of the enlarged local structure schematic diagrams of the present application;

[0022] Figure 4 It is another overall structure schematic diagram of the present application;

[0023] Figure 5 It is an overall structure schematic diagram of the limiting assembly;

[0024] Figure 6 It is one of the cross-sectional structure schematic diagrams of the present application; Figure 5 It is one of the cross-sectional structure schematic diagrams of the present application;

[0025] Figure 7 It is one of the overall structure schematic diagrams of the present application when the traction plates are connected;

[0026] Figure 8 It is one of the cross-sectional structure schematic diagrams of the present application; Figure 7 It is one of the cross-sectional structure schematic diagrams of the present application;

[0027] In the figure, 1, pad body; 11, cavity; 111, electric heating element; 112, lead wire; 113, joint; 12, surgical end; 13, contact end; 131, suction cup; 2, traction plate; 21, closed end; 211, connecting piece; 212, clamping area; 213, limiting protrusion; 22, traction end; 221, traction hook; 3, traction rope; 4, limiting assembly; 41, limiting rope; 411, restraint ring; 42, limiting buckle; 421, shell; 422, through hole; 423, elastic piece; 4231, button part; 4232, elastic part. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present application are clearly and completely described below through specific specific embodiments. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in the present application. The present application can also be implemented or applied by other different specific embodiments. In the case of no conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0029] An experimental animal bilateral common carotid artery surgical auxiliary device, such as Figures 1-8As shown, the device comprises: a pad 1; a traction plate 2, having mutually opposite closed ends 21 and traction ends 22, the closed ends 21 are provided with connecting pieces 211, when the two traction plates 2 are connected through the connecting pieces 211, a clamping area 212 is formed between the opposite closed ends 21; the traction ends 22 are provided with traction hooks 221; a traction rope 3, having two ends connected with the pad 1 and the traction plate 2 respectively. When the traction hooks 221 are hooked with the wound of the mouse neck, the traction rope 3 pulls the wound edges in opposite directions to keep the wound continuously open and expose the bilateral carotid arteries; when the closed ends 21 move towards each other and clamp the edges of the bilateral wounds, the connecting pieces 211 are connected with each other, at this time, the connecting pieces 211 overcome the driving force of the traction rope 3 on the traction plate 2 and clamp the wound in the clamping area 212. The traction rope 3 has elasticity; or, the length of the part of the traction rope 3 between the pad 1 and the traction plate 2 can be adjusted; or, the traction rope 3 is detachably connected with the pad 1, and when the two traction plates 2 are connected, the connection between one of the traction ropes 3 and the pad 1 is released. During the operation, the wound is pulled open in the opposite direction by the traction hooks 221 to obtain the operation field; in the interval between two unilateral operations, the fixation of the mouse limbs is released, and the wound is clamped and closed by the closed ends 21 of the traction plate 2, so that even if the mouse wakes up when the anesthetic effect is weakened, it can also move its limbs so that the medical staff can observe its physiological state, and the movement of the mouse will not cause the blood vessels or other tissues in the wound to come into contact with the external environment. At the same time, under the restriction of at least one traction rope 3, the mouse can also move within a certain range, effectively avoiding the complete restriction of movement which makes it produce fear or anxiety and other emotions that are not conducive to subsequent operation and postoperative recovery.

[0030] In some embodiments of the present application, as shown, Figures 1-8 As shown, the closed end 21 is also connected with a limiting protrusion 213, when the two traction plates 2 are connected through the connecting pieces 211, the clamping area 212 is between the limiting protrusion 213 and the connecting piece 211, at this time, the distance between the two limiting protrusions 213 is less than the distance between the two closed ends 21. The thickness of the mouse epidermis is in the range of 150-350 μm, according to the standard thickness of the C57BL / 6 mouse neck skin, the neck thickness is 150-180 μm, therefore, the distance between the two limiting protrusions 213 is not greater than 300 μm, which can extrude the edges of the wound inward when the closed ends 21 are closed. The distance between the two closed ends 21 is not greater than 400 μm, which can accommodate the edges of the wound without extruding them. Therefore, the purpose of the limiting protrusion 213 is to clamp the edges of the wound after they enter the clamping area 212, which is different from the clamping area 212: the limiting protrusion 213 is arranged in a continuous or dot matrix linear arrangement on the closed end 21, which reduces the contact area to increase the contact pressure, thereby improving the clamping effect on the wound; while the closed ends 21 on both sides of the clamping area 212 are parallel to each other, the purpose of their arrangement is to enable the part of the wound edges passing through the limiting protrusion 213 to maintain a relatively close shape.

[0031] In some embodiments of the present application, as shown in Figures 1-8 the inner part of the pad 1 has a cavity 11, an electric heating element 111 is connected in the cavity 11, and one end of a lead wire 112 is also connected to the electric heating element 111. The other end of the lead wire 112 is exposed outside the pad 1 and connected to a connector 113, which is specifically one of a type-C connector 113, a USB connector 113, a three-pin plug, or a two-pin plug. When the connector 113 is connected to a power source, the electric heating element 111 works and heats up. After the mouse is anesthetized, its body temperature regulation function is inhibited, and its body temperature will gradually decrease during the operation. Shaving and cleaning before the operation will make the heat loss situation worse. By setting the electric heating element 111 and the pad 1 capable of conducting heat, the mouse can be provided with a warming effect to slow down the loss of body temperature. The heating temperature of the pad 1 is not greater than 40°C.

[0032] In some embodiments of the present application, as shown in Figures 1-8 the pad 1 has an operation end 12, and the operation end 12 is connected to a limiting assembly 4, which includes a limiting rope 41 and a limiting buckle 42. One end of the limiting rope 41 is connected to the operation end 12, and the other end of the limiting rope 41 passes through the limiting buckle 42 twice and forms a constraint ring 411. When the limiting rope 41 and the limiting buckle 42 move relative to each other, the circumference of the constraint ring 411 changes. The traction rope 3 is connected to the operation end 12. The number of limiting assemblies 4 is not less than four. When the number of limiting assemblies 4 is four, they can be used to fix the four limbs of the mouse after it is anesthetized. By controlling the length of the part of the limiting rope 41 that passes through the limiting buckle 42 the second time, the size of the constraint ring 411 can be controlled, so that it can better adapt to the limbs of the experimental mouse. By controlling the length of the part of the limiting rope 41 that passes through the limiting buckle 42 the first time, the positional relationship between the limb of the mouse limited by the constraint ring 411 and the other end of the limiting rope 41 can be adjusted, so that the experimental mouse can be stably limited at the operation end 12 of the pad 1 according to its size. The number of limiting buckles 42 in each limiting assembly 4 is at least one. When the number of limiting buckles 42 exceeds one, they can play a role in collecting the limiting rope 41 and improving the limiting effect of the limiting rope 41.

[0033] In some embodiments of the present application, as shown in Figures 1-8As shown, the limiting buckle 42 comprises an outer shell 421 and an elastic member 423. The outer shell 421 is provided with at least two through holes 422. The elastic member 423 is connected to the inner wall of the outer shell 421. When the elastic member 423 is not subjected to external force, part of the elastic member 423 extends out of the outer shell 421 and blocks the through holes 422. When the elastic member 423 is deformed into an energy storage state under the driving of external force, the through holes 422 are restored to be in communication. The elastic member 423 has a button part 4231 and an elastic part 4232 connected to each other. When the elastic part 4232 is stretched, the button part 4231 extends out of the outer shell 421. The elastic part 4232 is a spring. The part of the button part 4231 in the outer shell 421 is the structure for locking and positioning the limiting rope 41 passing through the through holes 422.

[0034] In some embodiments of the present application, as shown in Figures 1-8 When the elastic member 423 extends outwards from the energy storage state, the limiting rope 41 passing through the through holes 422 is pressed against the inner wall of the outer shell 421. When the elastic member 423 is deformed under force to the energy storage state, the traction rope 3 can move relative to the through holes 422. At this time, the operator can pull the traction rope 3 and adjust the length of the part of the traction rope 3 passing through the through holes 422, so as to adjust the stretching degree of the traction rope 3 on the limbs of the mouse when fixing mice of different sizes. When the elastic member 423 is not subjected to external force, the circumference of the restraint ring 411 formed by the limiting rope 41 is constant, so that the fastening degree of the limbs of the mouse being surrounded by the restraint ring is unchanged, which can effectively prevent the mouse from slipping off from the surgical end 12 of the pad 1 when the pad 1 is placed on a slope with an angle.

[0035] In some embodiments of the present application, as shown in Figures 1-8 As shown, the pad 1 also has a contact end 13, which is away from the surgical end 12. The contact end 13 faces the plane in contact with the pad 1 when in operation. The contact end 13 is connected with a plurality of suction cups 131. The suction cups 131 are fixedly connected with the contact end 13. When the suction cups 131 are adsorbed on the surface of the environment, the pad 1 is parallel to the surface of the environment. At the same time, the distance between the contact end 13 and the surface of the environment is fixed, so as to ensure the stability of the connection between the pad 1 and the surface of the environment. When the medical staff performs fine operation on the arterial vessels of the mouse, the accuracy of the operation needs to be ensured. Finding an appropriate operation posture is an important factor to ensure the accuracy of the operation in a long time operation. The current mouse operation device is mainly placed on an operation table or an operating table and is relatively bulky, which is not conducive to being fixed on a plane with an angle and is not convenient for the medical staff to adjust the position according to the habitual posture during operation. By arranging the suction cups 131 on the contact end 13 of the pad 1, the pad 1 can be stably adsorbed on various planes. This is conducive to helping the medical staff to find the best operation posture by connecting the pad 1 to different planes.

[0036] In some embodiments of the present application, as shown inFigures 1-8 As shown, the width of the limiting protrusion 213 is greater than the width of the traction hook 221, and the mouse epidermis is pressed and deformed when the limiting protrusion 213 extrudes the wound edge; and the width of the limiting protrusion 213 is greater than the length of the wound, so that the wound edge can be completely clamped by the clamping area 212, and then the two sides of the wound are cut off. The traction hook 221 needs to partially extend into the wound during work, so the width of the traction hook 221 is less than the length of the wound. Specifically, the connecting piece 211 is a magnetic attraction structure, and the magnetic attraction structure is a dysprosium-iron-boron magnet symmetrically embedded in the outer wall of the two closed end portions 21; or, the connecting piece 211 is a clamping groove and a clamping block connected to the two closed end portions 21, which can be clamped when the two closed end portions 21 move towards each other, and at the same time, the limiting protrusion 213 can clamp the skin of the wound edge.

Claims

1. A surgical aid device for bilateral common carotid artery surgery in experimental animals, characterized in that, include, Cushion; A traction plate has a closed end and a traction end that are opposite to each other. The closed end is provided with a connector. When two traction plates are connected by the connector, a clamping area is formed between the opposite closed ends. The traction end is provided with a traction hook. The traction rope has two ends connected to the pad and the traction plate, respectively. When the traction hook is connected to the wound on the mouse's neck, the traction rope pulls the edge of the wound in the opposite direction to keep the wound open and expose the bilateral carotid arteries. When the closed ends move towards each other and clamp the edges of the wound on both sides, the connectors are connected to each other. At this time, the connectors overcome the drive of the traction rope on the traction plate and clamp the wound in the clamping area.

2. The experimental animal bilateral common carotid artery surgical aid device according to claim 1, characterized in that, The closed end is also connected to a limiting protrusion. When the two traction plates are connected by a connector, the clamping area is between the limiting protrusion and the connector. At this time, the distance between the limiting protrusions on both sides is less than the distance between the closed ends on both sides, and the distance between the limiting protrusions on both sides is no more than 5μm.

3. The experimental animal bilateral common carotid artery surgical aid device according to claim 1, characterized in that, The pad has an internal cavity, and an electric heating element is connected inside the cavity. One end of the electric heating element is also connected to a lead wire. The other end of the surgical lead wire passes through the pad, is exposed to the outside, and is connected to a connector. When the connector is connected to a power source, the electric heating element operates and heats up.

4. The experimental animal bilateral common carotid artery surgical aid device according to claim 3, characterized in that, The pad has a surgical end, which is connected to a limiting component, including a limiting rope and a limiting buckle. One end of the limiting rope is connected to the surgical end, and the other end of the limiting rope passes through the limiting buckle twice to form a constraint ring. When the limiting rope and the limiting buckle move relative to each other, the circumference of the constraint ring changes.

5. The experimental animal bilateral common carotid artery surgical aid device according to claim 4, characterized in that, The limiting buckle includes a shell and an elastic element. The shell has at least two through holes. The elastic element is connected to the inner wall of the shell. When the elastic element is not subjected to external force, it partially extends out of the shell and blocks the through holes. When the elastic element is driven by external force to deform into the shell to a storage state, the through holes are restored to open.

6. The experimental animal bilateral common carotid artery surgical aid device according to claim 5, characterized in that, When the elastic element extends outward from the energy storage state, it presses the limiting rope passing through the through hole against the inner wall of the outer shell. When the elastic element is deformed to the energy storage state, the traction rope can move relative to the through hole.

7. The experimental animal bilateral common carotid artery surgical aid device according to claim 3, characterized in that, The traction rope is connected to the surgical end.

8. The experimental animal bilateral common carotid artery surgical aid device according to claim 4, characterized in that, The number of limit buckles in each group of limit components is at least one.

9. The experimental animal bilateral common carotid artery surgical aid device according to claim 3, characterized in that, The pad also has a contact end, which is opposite to the surgical end. When in operation, the contact end faces the plane that contacts the pad, and the contact end is connected to several suction cups.

10. The experimental animal bilateral common carotid artery surgical aid device according to claim 2, characterized in that, The width of the limiting protrusion is greater than the width of the traction hook.

Images