Fluid three-dimensional mixer, application structure and manufacturing method of fluid three-dimensional mixer

Abstract

The invention discloses a fluid three-dimensional mixer, an application structure and a manufacturing method thereof, the fluid three-dimensional mixer comprises a liquid inlet channel, a vertical laminated mixing assembly and a liquid outlet channel which are communicated in sequence, the liquid inlet channel comprises a liquid inlet branch channel assembly and a liquid inlet mixing flow channel communicated with the liquid inlet branch channel assembly, the liquid inlet mixing flow channel is communicated with an inlet of the vertical laminated mixing assembly, and the liquid outlet channel is communicated with an outlet of the vertical laminated mixing assembly. The liquid inlet branch channel assembly comprises at least two liquid inlet branch channels communicated with the liquid inlet mixing flow channel; the vertical laminated mixing assembly comprises a plurality of laminated annular mixing channels and interlayer connecting channels communicated with the adjacent annular mixing channels, and an outlet of the vertical laminated mixing assembly is communicated with a liquid outlet channel. According to the invention, Dean vortex or secondary flow induction and three-dimensional space disturbance can be realized, chaotic flow is more easily formed under multiple actions, the multiphase microfluid molecule diffusion mixing capability is improved, and the microfluid mixing effect is enhanced; the application structure is expanded through series connection or / and parallel connection of a multi-branch system, and the purpose of complex mixed technological process is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic mixing, and in particular to a fluid three-dimensional mixer, an application structure and a manufacturing method thereof. BACKGROUND

[0002] In the biological, pharmaceutical, chemical, food, beverage, beauty, and other industries, with the improvement of product performance, production process requirements, and other aspects, high polymer microfluidic mixing at the micron or millimeter level has gradually become a bottleneck for technical development. Traditional mixers mostly use spiral stirring, two-dimensional plane disturbance, and other methods. Spiral stirring is also performed on the stirring plane, and the stirring plane is also a two-dimensional plane. Traditional mixers are more suitable for traditional material stirring and mixing, but they are not suitable for millimeter, micron, or even nanometer microfluidic mixing (such as LNP mixing). Traditional mixers will appear laminar flow and are not conducive to molecular diffusion. Research shows that traditional mixers are generally not suitable when the Reynolds number (Re) is less than 1000, and it is difficult to achieve the purpose of microfluidic mixing. The traditional microfluidic mixing has the following technical problems: 1. In the biological, pharmaceutical, chemical, food, beverage, beauty, and other industries, with the improvement of product performance, production process requirements, and other aspects, higher requirements are put forward for continuous mixing under complex conditions. 2. In the field of fluid continuous mixing, the traditional two-dimensional channel dominated mixing technology has significant performance bottlenecks. The problem lies in the flow characteristics limitation of two-dimensional plane structure, laminar flow dominance, and low diffusion efficiency. The flow of the three-dimensional channel is approximately laminar due to geometric constraints, and mixing mainly depends on molecular diffusion, making it difficult to meet the industrial requirements for mixing uniformity. 3. Insufficient shear and disturbance. The shear effect of the two-dimensional channel is limited to the plane direction, and it is difficult to effectively generate three-dimensional vortex flow or secondary flow. 4. Due to the problem of the stagnation boundary layer of the microchannel, the mass transfer efficiency significantly decreases under laminar flow conditions. 5. When facing complex fluid conditions, the two-dimensional channel method cannot solve the problems of high-viscosity fluid stagnation and mixing intensity. SUMMARY

[0003] The purpose of the present application is to provide a fluid three-dimensional mixer for microfluidic mixing, an application structure, and a manufacturing method thereof. The inlet channel achieves the purpose of several phase microfluidic inlet, and the three-dimensional channels of the interlayer connection channel of the vertical stacked mixing assembly, the ring-shaped mixing channel continuously flow, which facilitates the induction of Dean vortex or secondary flow and three-dimensional space disturbance, greatly promotes chaotic convection, increases mixing intensity, more easily forms chaotic flow under multiple actions, improves the molecular diffusion and mixing ability of multi-phase microfluidics, and enhances the microfluidic mixing effect.

[0004] The purpose of the present application is achieved by the following technical solutions: The fluid three-dimensional mixer comprises a liquid inlet channel, a vertical stacked mixing assembly and a liquid outlet channel which are sequentially communicated, the liquid inlet channel comprises a liquid inlet branch assembly and a liquid inlet mixing flow channel which is communicated with the liquid inlet branch assembly, the liquid inlet mixing flow channel is communicated with the inlet of the vertical stacked mixing assembly, the liquid inlet branch assembly comprises at least two liquid inlet branches which are communicated with the liquid inlet mixing flow channel, the vertical stacked mixing assembly comprises a plurality of stacked annular mixing channels and interlayer connecting channels which are communicated with adjacent annular mixing channels, and the outlet of the vertical stacked mixing assembly is communicated with the liquid outlet channel.

[0005] Preferably, the liquid inlet mixing flow channel is divided into laminar flow sub-channels according to the number of liquid inlet branches, and the laminar flow sub-channels are layered according to the flow direction of the liquid inlet mixing flow channel.

[0006] In order to better realize the present application, the present application further comprises N extension liquid inlet channels and N extension vertical stacked mixing assemblies, the extension liquid inlet channel comprises a liquid inlet branch assembly and a liquid inlet mixing flow channel which is communicated with the liquid inlet branch assembly, the liquid inlet mixing flow channel is communicated with the inlet of the vertical stacked mixing assembly, the liquid inlet branch assembly comprises at least two liquid inlet branches which are communicated with the liquid inlet mixing flow channel, and the extension vertical stacked mixing assembly has the same structure as the vertical stacked mixing assembly; the N extension liquid inlet channels and the N extension vertical stacked mixing assemblies are sequentially corresponded and grouped to form N groups of extension vertical mixing assembly bodies, the first group of extension vertical mixing assembly bodies in the N groups of extension vertical mixing assembly bodies is communicated with the vertical stacked mixing assembly, and the other extension vertical mixing assembly bodies in the N groups of extension vertical mixing assembly bodies are sequentially communicated, and the liquid outlet channel is arranged on the last group of extension vertical mixing assembly bodies in the N groups of extension vertical mixing assembly bodies.

[0007] Preferably, the interlayer connecting channels which connect adjacent annular mixing channels in the vertical stacked mixing assembly are in the shape of an "I", a "7", a bend or a snake, and the connecting position of the interlayer connecting channel and the annular mixing channel is on the upper end face, the lower end face, the inner side face or the outer side face of the annular mixing channel.

[0008] Preferably, the liquid inlet channel, the vertical stacked mixing assembly and the liquid outlet channel are sequentially and vertically communicated, and all the annular mixing channels in the vertical stacked mixing assembly are sequentially and vertically stacked communicated through the interlayer connecting channels.

[0009] Preferably, the connecting position of the interlayer connecting channel and the annular mixing channel is expanded in volume and is smoothly transitioned in the shape of a round corner, a taper angle, a trapezoidal channel or a C-shaped corner.

[0010] An application structure constituted by a fluid three-dimensional mixer, comprising annular mixing integrated discs corresponding to annular mixing channels, each annular mixing integrated disc is connected in sequence to constitute a vertical stacked mixing assembly, a recess is formed in the middle of the annular mixing integrated disc to form an annular mixing channel corresponding part corresponding to the annular mixing channel, the annular mixing channel corresponding part is connected to the integrated disc corresponding channel with a recess-shaped interlayer connecting channel by a channel in the center of the annular mixing integrated disc, the integrated disc corresponding channels of the interlayer connecting channel of the adjacent two annular mixing integrated discs are connected by the interlayer connecting channel, and the annular mixing channel corresponding part forms a cavity structure annular mixing channel after the adjacent two annular mixing integrated discs are stacked.

[0011] Preferably, the annular mixing integrated disc is provided with a sealing rubber ring around the annular mixing channel corresponding part, a notch-shaped reference position is formed on the side of the annular mixing integrated disc, and a plurality of connecting holes are formed on the annular mixing integrated disc; the interlayer connecting channel of the annular mixing integrated disc is a channel hole provided on the annular mixing integrated disc, and the channel hole connects the integrated disc corresponding channels of the interlayer connecting channels of the adjacent two annular mixing integrated discs.

[0012] Preferably, the annular mixing channel or / and the integrated disc corresponding channel of the interlayer connecting channel of the annular mixing integrated disc has an expansion part with expanded channel space, and the expansion part has a reinforced mixing body.

[0013] A manufacturing method for preparing a fluid three-dimensional mixer application structure, the method comprising: S1, manufacturing a plurality of annular mixing integrated discs corresponding to annular mixing channels, each annular mixing integrated disc is connected in sequence to constitute a vertical stacked mixing assembly, a recess is formed in the middle of the annular mixing integrated disc to form an annular mixing channel corresponding part corresponding to the annular mixing channel, the annular mixing channel corresponding part is connected to the integrated disc corresponding channel with a recess-shaped interlayer connecting channel by a channel in the center of the annular mixing integrated disc, the integrated disc corresponding channels of the interlayer connecting channel of the adjacent two annular mixing integrated discs are connected by the interlayer connecting channel, and the annular mixing channel corresponding part forms a cavity structure annular mixing channel after the adjacent two annular mixing integrated discs are stacked; S2, manufacturing liquid inlet integrated disc corresponding to liquid inlet channel, liquid inlet channel is provided through in liquid inlet integrated disc, liquid inlet channel includes liquid inlet branch channel assembly and liquid inlet mixing flow channel communicated with liquid inlet branch channel assembly, liquid inlet mixing flow channel is communicated with vertical layering mixing assembly inlet of first annular mixing integrated disc of vertical layering mixing assembly, liquid inlet branch channel assembly contains at least two liquid inlet branch channels communicated with liquid inlet mixing flow channel, and the disc surface of liquid inlet integrated disc has liquid inlet branch channel port corresponding to liquid inlet branch channel;Manufacturing liquid outlet integrated disc corresponding to liquid outlet channel, through liquid outlet channel is opened in liquid outlet integrated disc, and the disc surface of liquid outlet channel has liquid outlet channel port corresponding to liquid outlet channel, and the outlet of last annular mixing integrated disc of vertical layering mixing assembly is communicated with liquid outlet channel; S3, liquid inlet integrated disc, vertical layering mixing assembly communicated by a plurality of annular mixing integrated discs and liquid outlet integrated disc are sequentially communicated and connected to be integrated into one structure.

[0014] Preferably, the annular mixing integrated disc is manufactured by methods including 3D printing, machining, etching, lithography and injection molding, each annular mixing integrated disc is combined into vertical layering mixing assembly by layering assembly method, and the structure is applied to mixing of flowing fluid, particle mixing or / and LNP synthesis including biology, medicine, chemical industry, food, beverage and beauty.

[0015] Compared with the prior art, the present application has the following advantages and beneficial effects: (1) the liquid inlet channel of the present application realizes the purpose of several phase microfluids, the three-dimensional channels of the interlayer connection channels of the vertical layering mixing assembly and the annular mixing channel continuously flow, which facilitates the induction of Dean vortex or secondary flow and three-dimensional space disturbance, greatly promotes chaotic convection, increases mixing intensity, more easily forms chaotic flow under multiple actions, improves the diffusion and mixing capacity of multi-phase microfluids and enhances the microfluid mixing effect.

[0016] (2) the present application realizes the mixing of front and rear microfluids in turn and then mixing, and realizes the mixing operation of multiple microfluids after mixing according to process requirements, so that the purpose of complex mixing process flow of biological medicine, food and chemical industry can be realized. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the structure schematic view of the first fluid three-dimensional mixer in the embodiment; Figure 2 It is the structure schematic view of the second fluid three-dimensional mixer in the embodiment; Figure 3 It is the local sectional structure schematic view of the fluid three-dimensional mixer in the embodiment; Figure 4Here are two examples of simulation diagrams illustrating the inlet fluid in the inlet channel for microfluidics. Figure 5 This is a schematic diagram of the structure of the third type of three-dimensional fluid mixer in the embodiment; Figure 6 This is a schematic diagram of the fourth type of three-dimensional fluid mixer in the embodiments; Figure 7 This is a schematic diagram of the fifth type of three-dimensional fluid mixer in the embodiments; Figure 8 This is a schematic diagram of the circumferential mixing integrated disk structure in the fluid three-dimensional mixer application structure in the embodiment; Figure 9 These are schematic diagrams of four structures used to change local flow resistance in the embodiments; Figure 10 In order to be in Figure 9 The middle part is a schematic diagram of the principle after changing the local flow resistance application combination; Figure 11 These are four schematic diagrams showing the connection points between the interlayer connection channel and the circumferential mixing channel in the embodiments; Figure 12 for Figure 11 A schematic diagram illustrating the usage state of a fluid three-dimensional mixer. Figure 13 for Figure 5 A schematic diagram of the assembly of a medium-fluid three-dimensional mixer; Figure 14 The following is a schematic diagram illustrating the structure of a vertically stacked hybrid component consisting of three circumferentially oriented hybrid integrated disks, as exemplified in this embodiment. Figure 15 An exploded view of the structure of the first type of three-dimensional fluid mixer application, as exemplified in the embodiments; Figure 16 for Figure 15 A schematic diagram of the assembled structure; Figure 17 for Figure 15 A schematic diagram of the formation of the test sample; Figure 18 This is a test diagram of the synthesis effect in the LNP mixing experiment in the example; Figure 19 This is a schematic diagram of a circumferential hybrid integrated disk extension structure in one embodiment; Figure 20 An exploded view of the structure of a second type of three-dimensional fluid mixer application, as exemplified in the embodiments; Figure 21 The following is a schematic diagram illustrating the principle of two mixers assembled for an example application; Figure 22 This is a microfluidic mixing simulation diagram of one layout configuration in the embodiment.

[0018] The names corresponding to the reference numerals in the attached figures are as follows: 1 - Inlet channel, 11 - Inlet branch A, 12 - Inlet branch B, 13 - Inlet branch C, 111 First type of microfluidic, 112 Second type of microfluidic, 2 - Inlet mixing channel, 3 - Vertical stacked mixing assembly, 4 - Circumferential mixing channel, 41 - Bifurcation loop A, 42 - Bifurcation loop B, 5 - Interlayer connection channel, 51 - Interlayer connection channel A, 52 - Interlayer connection channel B, 53 - Interlayer connection channel C, 6 - Outlet channel, 7 - Expansion section, 71 - Reinforced hybrid body, 8 - Extended vertical stacked hybrid assembly, 10 - Circumferential hybrid integration disk, 101 - Reference position, 102 - Sealing ring, 103 - Connection hole, 40 - Corresponding part of circumferential hybrid channel, 50 - Corresponding channel of interlayer connection channel integration disk, 1001 Rounded corner, 1002 C-shaped corner, 1021 Expansion section A at the connection position, 1003 Taper angle, 1031 Expansion section B at the connection position, 1004 Trapezoidal channel. Detailed Implementation

[0019] The present invention will be further described in detail below with reference to embodiments: Example 1 like Figure 1 As shown, a three-dimensional fluid mixer includes an inlet channel 1, a vertically stacked mixing assembly 3, and an outlet channel 6 connected in sequence. The inlet channel 1 includes an inlet branch assembly and an inlet mixing channel 2 connected to the inlet branch assembly. The inlet mixing channel 2 is connected to the inlet of the vertically stacked mixing assembly 3 (the vertically stacked mixing assembly 3 is a mixing assembly, see [reference]). Figure 1 The vertically stacked mixing component 3 (with the top as the inlet and the bottom as the outlet) is connected, and the liquid inlet branch assembly includes at least two liquid inlet branches connected to the liquid inlet mixing channel 2. Taking an inlet branch assembly with two liquid inlet branches as an example, see [reference needed]. Figure 1 The two inlet branches of the inlet branch assembly and the inlet mixing channel 2 form a "Y" shape; see also Figure 2 The two inlet branches of the inlet branch assembly and the inlet mixing channel 2 form a "T" shape; see [link / reference]. Figure 5 The two inlet branches of the inlet branch assembly are arranged in a "U" shape, and then the inlet mixing channel 2 is connected to the middle of the inlet branch assembly. Figure 6 The inlet branch assembly is Figure 5 The changing structural forms.

[0020] like Figures 1-6As shown, the vertical stacked mixing assembly 3 includes several stacked ring-shaped mixing channels 4 and interlayer connecting channels 5 connecting adjacent ring-shaped mixing channels 4, and the vertical stacked mixing assembly 3 is connected with a liquid outlet channel 6 at the outlet. The interlayer connecting channels 5 connecting adjacent ring-shaped mixing channels 4 in the vertical stacked mixing assembly 3 are in the shape of "I", "7", bending or serpentine, and the connecting position of the interlayer connecting channels 5 and the ring-shaped mixing channels 4 is at the upper end face or lower end face or inner side face or outer side face of the ring-shaped mixing channels 4. Figures 1-6 Various application change schematic diagrams of different liquid inlet branch assembly, interlayer connecting channel 5 structure and connecting position are respectively shown. Referring to Figure 6 , the liquid inlet branch assembly of the liquid inlet channel 1 includes a liquid inlet branch A11 and a liquid inlet branch B12, the liquid inlet branch A11 flows in the first microfluid 111, and the liquid inlet branch B12 flows in the second microfluid 112. In some embodiments, the ring-shaped mixing channel 4 of the present application can adopt the structure form of Figure 9 , the ring-shaped mixing channel 4 enters the two-phase microfluid and molecular mixing through the interlayer connecting channel 5 (corresponding channel 50 of the interlayer connecting channel integrated disc in Figure 9 ), and the two-phase microfluid flows in the bifurcated ring channel A41 and the bifurcated ring channel B42 respectively while being molecularly mixed, and the bifurcated ring channel A41 and the bifurcated ring channel B42 of the ring-shaped mixing channel 4 flow to the ring-shaped mixing channel 4 of the next layer at the other end or the other side (see Figure 6 ), and the ring-shaped mixing channel 4 occurs again at this position, which is more conducive to inducing Dean vortex or secondary flow, greatly promoting chaotic convection, increasing the mixing strength, and the interlayer connecting channel 5 of the ring-shaped mixing channel 4 flowing to the ring-shaped mixing channel 4 of the next layer further promotes the induction of Dean vortex or secondary flow, so that the two-phase microfluid can maintain molecular diffusion interaction at any time, greatly enhancing the three-dimensional spatial disturbance effect and enhancing the microfluid mixing effect.

[0021] In some embodiments, the liquid inlet mixing flow channel 2 is divided into layer flow channels according to the number of liquid inlet branches, and the layer flow channels are layered according to the flow direction of the liquid inlet mixing flow channel 2; as shown in Figure 4 , taking the case that the liquid inlet branch assembly includes two liquid inlet branches (i.e., including a liquid inlet branch A and a liquid inlet branch B), Figure 4The two liquid inlet branch channels of the liquid inlet branch channel assembly are arranged in a "Y" shape, the first kind of microfluid 111 flows into the liquid inlet branch channel A, and the second kind of microfluid 112 flows into the liquid inlet branch channel B. The first kind of microfluid 111 and the second kind of microfluid 112 are left-right symmetrical in the plane where the liquid inlet branch channel assembly is located. The liquid inlet mixing flow channel 2 is in the shape of "L" as a whole. The first kind of microfluid 111 and the second kind of microfluid 112 are mostly layered in the vertical direction (partially molecular communication) in the liquid inlet mixing flow channel 2. The first kind of microfluid 111 and the second kind of microfluid 112 are mostly layered in the vertical direction (partially molecular communication) in the first ring-shaped mixing channel 4 of the vertical layering mixing assembly 3. Then, the three-dimensional channels of the interlayer connecting channel 5 and the ring-shaped mixing channel 4 of the vertical layering mixing assembly 3 continuously flow (including translation, rotation, tumbling and other multidirectional movements, which facilitates the induction of Dean vortex or secondary flow). The three-dimensional space disturbance (compared with the traditional mixer, the present application forms a multidirectional movement disturbance including horizontal and vertical movements in a limited space, breaks the symmetry of laminar flow, greatly promotes chaotic convection, and increases the mixing intensity). Under the action of multiple factors, chaotic flow is more easily formed, so that the two-phase microfluid can maintain molecular diffusion interaction at any time, and the microfluid mixing effect is enhanced.

[0022] Referring to Figures 1-13 , the liquid inlet channel 1, the vertical layering mixing assembly 3 and the liquid outlet channel 6 are sequentially and vertically connected. All the ring-shaped mixing channels 4 in the vertical layering mixing assembly 3 are sequentially and vertically connected through the interlayer connecting channel 5. The adjacent two ring-shaped mixing channels 4 are connected through the interlayer connecting channel 5.

[0023] In some embodiments, as shown in Figure 10 , the volume of the channel at the connection position of the interlayer connecting channel 5 and the ring-shaped mixing channel 4 is expanded and smoothly transitions through a round corner, a taper angle, a trapezoidal channel or a C-shaped corner. As shown in Figure 11 , in the a diagram of Figure 11 , the volume of the channel at the connection position is expanded and smoothly transitions through a round corner 1001 at the connection position. In the b diagram of Figure 11 , the volume of the channel at the connection position is expanded and smoothly transitions through a C-shaped corner 1002 at the connection position. The C-shaped corner 1002 is designed to be convex and increase the volume of the channel to expand the channel. In the c diagram of Figure 11 , the volume of the channel at the connection position is expanded and smoothly transitions through a taper angle 1003 at the connection position. The taper angle 1003 is designed to be convex and increase the volume of the channel to expand the channel. In the d diagram of Figure 11 , the volume of the channel at the connection position is expanded.

[0024] Embodiment Two The embodiment one mainly introduces a liquid inlet channel 1, a vertical stacked mixing assembly 3 and a liquid outlet channel 6 to constitute a fluid three-dimensional mixing single system (to realize the mixing of the liquid inlet channel 1 into multiple microfluids in the single system), the embodiment includes the fluid three-dimensional mixing single system of the embodiment one, and further includes N groups of expanded vertical mixing assembly bodies (each group of the expanded vertical mixing assembly body constitutes a fluid three-dimensional mixing branch system, the fluid three-dimensional mixing branch system realizes the mixing of multiple microfluids in a branch, and then is connected in series with the fluid three-dimensional mixing single system, the fluid three-dimensional mixing single system and the N groups of the expanded vertical mixing assembly bodies are connected in series in turn, the microfluids are mixed separately and then are mixed in turn, and then the multiple microfluids are mixed according to the process requirements, so that the complex mixing process flow purposes of biological medical treatment, food and chemical industry and the like can be realized). Compared with the embodiment one, specifically, the fluid three-dimensional mixer further includes N expanded liquid inlet channels and N expanded vertical stacked mixing assemblies 8, the expanded liquid inlet channel includes a liquid inlet branch assembly and a liquid inlet mixing flow channel 2 connected with the liquid inlet branch assembly, the liquid inlet mixing flow channel 2 is connected with the inlet of the vertical stacked mixing assembly 3, the liquid inlet branch assembly includes at least two liquid inlet branches connected with the liquid inlet mixing flow channel 2, and the expanded vertical stacked mixing assembly 8 has the same structure as the vertical stacked mixing assembly 3 (the expanded vertical stacked mixing assembly 8 also includes a plurality of stacked ring-shaped mixing channels 4 and interlayer connecting channels 5 connecting adjacent ring-shaped mixing channels 4). The expanded liquid inlet channel can have the same structure as the liquid inlet channel 1 of the fluid three-dimensional mixing single system, so as to realize the liquid inlet and mixing of multiple microfluids (such as another two-phase microfluid or only one-phase microfluid), if only one-phase microfluid, only the microfluid inlet is performed, if multiple microfluids (such as another two-phase microfluid), the liquid inlet and mixing (preliminary molecular mixing) of the multiple microfluids are realized. The N expanded liquid inlet channels and the N expanded vertical stacked mixing assemblies 8 are sequentially corresponded and connected to constitute N groups of expanded vertical mixing assembly bodies (one group of the expanded vertical mixing assembly body constitutes a fluid three-dimensional mixing branch system), the first group of the expanded vertical mixing assembly body in the N groups of the expanded vertical mixing assembly bodies is connected with the vertical stacked mixing assembly 3, and the other expanded vertical mixing assembly bodies in the N groups of the expanded vertical mixing assembly bodies are sequentially connected (preferably, the fluid three-dimensional mixing single system and the N groups of the expanded vertical mixing assembly bodies are sequentially connected in series; of course, according to the process requirements, the fluid three-dimensional mixing single system and the N groups of the expanded vertical mixing assembly bodies can be connected in series or / and in parallel, and the mixing operation can be performed according to the complex process requirements, and the parallel connection is simply shown as follows: one component of a plurality of fluid three-dimensional mixing branch systems is connected in parallel with another component of a plurality of fluid three-dimensional mixing branch systems, and then is connected in series or in parallel with a third component of a plurality of fluid three-dimensional mixing branch systems), and the liquid outlet channel 6 is arranged on the last group of the expanded vertical mixing assembly body in the N groups of the expanded vertical mixing assembly bodies. Referring to Figure 7 ,Figure 7 For a principle example, the inlet channel 1 communicates with the vertical stacked mixing assembly 3 to form a fluid three-dimensional mixing single system, and the example only includes one fluid three-dimensional mixing branch system (which does not carry out mixing and relies on the inlet branch channel C13 to enter the third microfluid), the inlet branch channel assembly of the inlet channel 1 includes the inlet branch channel A11 and the inlet branch channel B12, the first microfluid 111 flows into the inlet branch channel A11, and the second microfluid 112 flows into the inlet branch channel B12. The first microfluid 111 and the second microfluid 112 carry out three-dimensional microfluid mixing in the fluid three-dimensional mixing single system, and then carry out three-dimensional microfluid mixing with the third microfluid in the expanded vertical stacked mixing assembly 8, and then the mixed microfluid mixture after mixing is discharged through the outlet channel 6. Referring to Figure 12 , the embodiment adopts the combined structure of the inlet branch channel assembly and the interlayer connecting channel to carry out two-phase microfluid mixing, and for example, two kinds of microfluid are mixed, which have reached a complete mixing state (green represents that the two-phase microfluid is in a mixing state) when the fluid enters the third ring (i.e. the ring-shaped mixing channel).

[0025] Embodiment three As shown in Figures 8-10 and Figures 14-21 , an application structure formed or made according to the fluid three-dimensional mixer, the application structure of the embodiment is creatively developed under the structure principle of the fluid three-dimensional mixer of the embodiment one or the embodiment two (the application structure is a creative structure under the structure idea of the fluid three-dimensional mixer, and belongs to the same inventive concept), and the application structure of the fluid three-dimensional mixer includes the ring-shaped mixing integrated disc 10 corresponding to the ring-shaped mixing channel 4, each ring-shaped mixing integrated disc 10 is connected in sequence to form the vertical stacked mixing assembly 3, the middle groove of the ring-shaped mixing integrated disc 10 forms the ring-shaped mixing channel corresponding part 40 corresponding to the ring-shaped mixing channel 4 (the ring-shaped mixing integrated disc 10 is an innovative part corresponding to the ring-shaped mixing channel 4, and the ring-shaped mixing channel corresponding part 40 corresponds to the channel inside the ring-shaped mixing channel 4), the ring-shaped mixing channel corresponding part 40 is connected to the groove-shaped interlayer connecting channel integrated disc corresponding channel 50 towards the center of the ring-shaped mixing integrated disc 10 (the interlayer connecting channel integrated disc corresponding channel 50 is a microfluid inflow or outflow channel of the ring-shaped mixing channel corresponding part 40), and the interlayer connecting channel integrated disc corresponding channels 50 of the adjacent two ring-shaped mixing integrated discs 10 are connected through the interlayer connecting channel 5 (the ring-shaped mixing channel corresponding parts 40 of the adjacent two ring-shaped mixing integrated discs 10 are also connected through the interlayer connecting channel 5; the ring-shaped mixing channel corresponding part 40 of the ring-shaped mixing integrated disc 10 is connected to the interlayer connecting channel integrated disc corresponding channel 50 through the interlayer connecting channel 5, and the interlayer connecting channel integrated disc corresponding channel 50 is connected to the ring-shaped mixing channel corresponding part 40 of the next ring-shaped mixing integrated disc 10 through the interlayer connecting channel 5, and so on, and the ring-shaped mixing channel corresponding part 40 of the last ring-shaped mixing integrated disc 10 is connected to the outlet channel 6 through the interlayer connecting channel 5). Figure 8 For a simple example, the microfluid in the ring-shaped mixing integrated disc 10 of the last layer flows into the interlayer connecting channel integrated disc corresponding channel 50 through the interlayer connecting channel 5, and the interlayer connecting channel integrated disc corresponding channel 50 is connected to the outlet channel 6 through the interlayer connecting channel 5. Figure 9The flow is split to form a chaotic flow, enabling the two-phase microfluidics to maintain molecular diffusion interactions at all times; the interlayer connection channel 5 of the circumferential mixing integrated disk 10 is a channel hole disposed in the circumferential mixing integrated disk 10, which is not shown in the figure. The interlayer connection channel integrated disk corresponds to the channel 50 according to... Figure 9 After being split, the flow converges to form a chaotic flow and is transported through the channel hole to the next layer of interlayer connecting channel integration disk corresponding to channel 50 or circumferential mixing channel corresponding part 40. After two adjacent circumferential mixing integration disks 10 are stacked, the circumferential mixing channel corresponding part 40 forms a cavity structure circumferential mixing channel 4. In some embodiments, such as Figure 15 As shown, the liquid inlet channel 1 is integrated on the cover plate. The liquid inlet branches A11 and B12 of the liquid inlet channel 1 correspond to Luer connectors (pipe connectors) respectively. The channel upper plate, channel plate, and matching screws serve as mounting fittings. Each modular component is a circumferential mixing integrated disc 10, and the liquid inlet is the liquid outlet channel 6. Each modular component and liquid inlet can be installed in the mounting block. This assembly forms a compact and novel three-dimensional fluid mixer application structure. Figure 16 The left image shows the product's application structure after assembly and molding. Figure 16 The figure on the right is the corresponding structural sectional view; Figure 17 According to this embodiment Figure 16 The prepared sample was used in a synthesis test in an LNP mixing experiment, and the results were as follows: Figure 18 As shown. Similarly, following the structural concept of the fluid three-dimensional mixer application of this invention, a [structure / engineering] can be manufactured. Figure 20 The illustrated fluid three-dimensional mixer application structure product. In some embodiments, during actual use, the circumferential mixing integrated disk 10 ( Figure 19 (Left image) can be paired with a cover plate ( Figure 19 (See right figure) to achieve the formation of the closed microfluidic channel corresponding to the circumferential mixing channel 4.

[0026] In some embodiments, a sealing ring 102 is installed around the corresponding portion 40 of the circumferential mixing channel of the circumferential mixing integrated disk 10, and a notch-shaped reference position 101 is opened on the side of the circumferential mixing integrated disk 10 (see 14). Figure 14 For example, consider three circumferential hybrid integrated disks 10, each with a reference position 101. The three circumferential hybrid integrated disks 10 are aligned and installed using the reference positions 101. Several connection holes 103 are formed on each circumferential hybrid integrated disk 10. The interlayer connection channel 5 of the circumferential hybrid integrated disk 10 is a channel hole provided in the circumferential hybrid integrated disk 10 (the channel hole is not shown in the figure; the channel hole serves to connect the corresponding circumferential hybrid channel 40 of adjacent circumferential hybrid integrated disks 10). The channel hole connects the corresponding channel 50 of the interlayer connection channel integrated disks of two adjacent circumferential hybrid integrated disks 10.

[0027] In some embodiments, such asFigure 10 As shown, the annular mixing channel 4 of the annular mixing integrated disc 10 or / and the corresponding channel 50 of the interlayer connection channel integrated disc has an expansion part 7 with increased channel space expansion, and the expansion part 7 has a reinforced mixing body 71.

[0028] This embodiment mainly introduces a liquid inlet channel 1, a set of vertical layer-stacked mixing assemblies 3, and a liquid outlet channel 6 to form a fluid three-dimensional mixing single system (to realize the mixing of the liquid inlet channel 1 into a plurality of microfluidic single systems), and a plurality of fluid three-dimensional mixing branch systems (i.e., N groups of expanded vertical mixing assembly bodies) can also be constructed according to Embodiment 2, see Figure 21 According to the application, the fluid three-dimensional mixer structure is applied to structure a plurality of fluid three-dimensional mixing branch systems for multiphase microfluidic molecular mixing processing.

[0029] A manufacturing method of an application structure of a fluid three-dimensional mixer, which is manufactured by methods including 3D printing, machining, etching, photolithography, and injection molding mold opening, and each annular mixing integrated disc is combined into a vertical layer-stacked mixing assembly by a layer-stacking assembly method, and the application structure is used for mixing of flowing fluids, particle mixing, or / and LNP synthesis in biological, medical, chemical, food, beverage, and beauty industries.

[0030] As shown in Figures 1-22As shown, this embodiment uses the fluid three-dimensional mixer of the present application to mix LNP (i.e., Lipid Nanoparticle) as an example, and the following is an example formula: Organic phase formula (ethanol system), lipid composition: cationic lipid DLin-MC3-DMA: 50 mol%; auxiliary lipid DSPC: 10 mol%; cholesterol: 38.5 mol%; PEG lipid DMG-PEG 2000: 1.5 mol%; solvent: anhydrous ethanol; total lipid concentration: 6-8 mM. Water phase formula (buffer system), buffer: 10 mM sodium citrate buffer; salt concentration: 50 mM NaCl; additive: 0.01% Pluronic F-68. The flow rate ratio of this embodiment is as follows: water phase: organic phase = 3:1; test flow rate: 50 mL / min. The detection instrument used is a nanoparticle size potential analyzer, and the detection parameters are: temperature: 25°C, detection angle: 173° (back scattering mode); measurement times: 5 repetitions. Connect the syringe (syringe corresponding to each inlet branch of the inlet branch assembly) to the mixer; set the water phase and lipid phase ratio to 3:1 in the syringe pump, and test at a total flow rate of 50 ml / min. During the test, the first 5 seconds of liquid are discarded, and then 10 milliliters of liquid are collected using a core tube. Use a pipette to add the mixed liquid to a cuvette. Run 3 independent measurements, each for 60 seconds, and record the PDI and particle size distribution curve. The particle size and PDI distribution measured by this method are: average particle size: 93.55 nm (industry generally within the specification range of 50-150 nm), PDI average 0.052 (industry generally PDI <0.2 is qualified, this embodiment can achieve 0.052, the effect is very significant).

[0031] Example Four A manufacturing method for manufacturing a fluid three-dimensional mixer application structure, the method comprising: S1, manufacturing a plurality of ring-shaped mixing integrated discs 10 corresponding to the ring-shaped mixing channels 4, each ring-shaped mixing integrated disc 10 is sequentially stacked and connected to form a vertical stacked mixing assembly 3, a middle groove in the ring-shaped mixing integrated disc 10 forms a ring-shaped mixing channel corresponding part 40 corresponding to the ring-shaped mixing channel 4, the ring-shaped mixing channel corresponding part 40 is connected to the groove-shaped interlayer connection channel integrated disc corresponding channel 50 in the center of the ring-shaped mixing integrated disc 10, the interlayer connection channel integrated disc corresponding channel 50 of the adjacent two ring-shaped mixing integrated discs 10 is connected through the interlayer connection channel 5, and the ring-shaped mixing channel corresponding part 40 of the adjacent two ring-shaped mixing integrated discs 10 forms a cavity structure ring-shaped mixing channel 4 after being stacked.

[0032] S2, manufacturing the liquid inlet integrated disc corresponding to the liquid inlet channel 1, the liquid inlet integrated disc is internally provided with the liquid inlet channel 1, the liquid inlet channel 1 comprises a liquid inlet branch assembly and a liquid inlet mixing flow channel 2 in communication with the liquid inlet branch assembly, the liquid inlet mixing flow channel 2 is in communication with the inlet of the vertical laminated mixing assembly 3 of the first annular mixing integrated disc 10, the liquid inlet branch assembly comprises at least two liquid inlet branches in communication with the liquid inlet mixing flow channel 2, the liquid inlet integrated disc is provided with liquid inlet branch ports corresponding to the liquid inlet branches on the disc surface. Manufacturing the liquid outlet integrated disc corresponding to the liquid outlet channel 6, the liquid outlet integrated disc is internally provided with the liquid outlet channel 6, the disc surface of the liquid outlet channel 6 is provided with liquid outlet channel ports corresponding to the liquid outlet channel 6, the outlet of the last annular mixing integrated disc 10 of the vertical laminated mixing assembly 3 is in communication with the liquid outlet channel 6.

[0033] S3, sequentially connecting the liquid inlet integrated disc, the vertical laminated mixing assembly 3 composed of a plurality of annular mixing integrated discs 10 in communication and the liquid outlet integrated disc to form an integrated structure.

[0034] The manufacturing method according to the present application can manufacture the fluid three-dimensional mixer application structure of a fluid three-dimensional mixing single system, and can also manufacture the fluid three-dimensional mixer application structure comprising a plurality of fluid three-dimensional mixing branch systems (namely N groups of expanded vertical mixing assembly bodies).

[0035] The above only describes the preferred embodiments of the present application and is not used to limit the present application, any modification, equivalent replacement and improvement within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A three-dimensional fluid mixer, characterized in that: The device includes a liquid inlet channel, a vertically stacked mixing assembly, and a liquid outlet channel connected in sequence. The liquid inlet channel includes a liquid inlet branch assembly and a liquid inlet mixing channel connected to the liquid inlet branch assembly. The liquid inlet mixing channel is connected to the inlet of the vertically stacked mixing assembly. The liquid inlet branch assembly includes at least two liquid inlet branches connected to the liquid inlet mixing channel. The vertically stacked mixing assembly includes several stacked circumferential mixing channels and interlayer connecting channels connecting adjacent circumferential mixing channels. The outlet of the vertically stacked mixing assembly is connected to the liquid outlet channel.

2. The three-dimensional fluid mixer according to claim 1, characterized in that: It also includes N extended liquid inlet channels and N extended vertical stacked mixing components. The extended liquid inlet channel includes a liquid inlet branch component and a liquid inlet mixing channel connected to the liquid inlet branch component. The liquid inlet mixing channel is connected to the inlet of the vertical stacked mixing component. The liquid inlet branch component includes at least two liquid inlet branches connected to the liquid inlet mixing channel. The extended vertical stacked mixing component has the same structure as the vertical stacked mixing component. The N extended liquid inlet channels and the N extended vertical stacked mixing components are sequentially grouped and connected to form N groups of extended vertical mixing component bodies. The first group of extended vertical mixing component bodies in the N groups of extended vertical mixing component bodies is connected to the vertical stacked mixing component. The other extended vertical mixing component bodies in the N groups of extended vertical mixing component bodies are sequentially connected. The liquid outlet channel is connected and disposed on the last group of extended vertical mixing component bodies in the N groups of extended vertical mixing component bodies.

3. The three-dimensional fluid mixer according to claim 1 or 2, characterized in that: The interlayer connection channel connecting adjacent circumferential mixing channels in the vertical stacked mixing assembly is in the shape of an "I", a "7", a bend, or a snake. The connection position between the interlayer connection channel and the circumferential mixing channel is on the upper end face, lower end face, inner side, or outer side of the circumferential mixing channel.

4. The three-dimensional fluid mixer according to claim 1, characterized in that: The liquid inlet channel, the vertically stacked mixing component, and the liquid outlet channel are arranged vertically in sequence. All circumferential mixing channels in the vertically stacked mixing component are connected vertically in sequence through interlayer connecting channels.

5. The three-dimensional fluid mixer according to claim 1, characterized in that: The interlayer connecting channel and the circumferential mixing channel are connected at the connection point with the channel volume expanding and smooth transition using rounded corners, tapered angles, trapezoidal channel shapes or C-shaped angles.

6. An application structure comprising a three-dimensional fluid mixer according to any one of claims 1 to 5, characterized in that: The assembly includes a circumferential mixing integrated disk corresponding to a circumferential mixing channel. Each circumferential mixing integrated disk is sequentially stacked to form the vertical stacked mixing component. The groove in the center of the circumferential mixing integrated disk forms a corresponding part of the circumferential mixing channel. The corresponding part of the circumferential mixing channel is connected to the corresponding channel of the interlayer connecting channel integrated disk with a groove shape towards the center of the circumferential mixing integrated disk. The corresponding channels of the interlayer connecting channel integrated disks of two adjacent circumferential mixing integrated disks are connected through the interlayer connecting channel. After two adjacent circumferential mixing integrated disks are stacked, the corresponding part of the circumferential mixing channel forms a cavity structure circumferential mixing channel.

7. The application structure according to claim 6, characterized in that: The circumferential mixing integrated disk is equipped with a sealing ring corresponding to the circumferential mixing channel. The circumferential mixing integrated disk has a notch-shaped reference position on its side and several connection holes. The interlayer connection channel of the circumferential mixing integrated disk is a channel hole set in the circumferential mixing integrated disk, and the channel hole connects the corresponding channel of the interlayer connection channel of two adjacent circumferential mixing integrated disks.

8. The application structure according to claim 6, characterized in that: The circumferential mixing channel and / or interlayer connection channel of the circumferential mixing integrated disk have an expansion portion with increased channel space, and the expansion portion contains a reinforcing mixing body.

9. A method for manufacturing an application structure for the three-dimensional fluid mixer of claim 6, characterized in that: The methods include: S1. Manufacture a plurality of annular mixing integrated disks corresponding to the annular mixing channels. Each annular mixing integrated disk is stacked and connected in sequence to form the vertical stacked mixing assembly. The groove in the middle of the annular mixing integrated disk forms an annular mixing channel corresponding part corresponding to the annular mixing channel. The annular mixing channel corresponding part is connected to the groove-shaped interlayer connecting channel integrated disk corresponding channel towards the center of the annular mixing integrated disk. The interlayer connecting channel integrated disk corresponding channels of two adjacent annular mixing integrated disks are connected through the interlayer connecting channel. After two adjacent annular mixing integrated disks are stacked, the annular mixing channel corresponding part forms an annular mixing channel with a cavity structure. S2. Manufacture an inlet integrated plate corresponding to the inlet channel. The inlet integrated plate has an inlet channel running through it. The inlet channel includes an inlet branch assembly and an inlet mixing channel connected to the inlet branch assembly. The inlet mixing channel is connected to the inlet of the first circumferential mixing integrated plate of the vertical stacked mixing assembly. The inlet branch assembly includes at least two inlet branches connected to the inlet mixing channel. The surface of the inlet integrated plate has inlet branch openings corresponding to the inlet branches. Manufacture an outlet integrated plate corresponding to the outlet channel. The outlet integrated plate has an outlet channel running through it. The surface of the outlet channel has an outlet channel opening corresponding to the outlet channel. The outlet of the last circumferential mixing integrated plate of the vertical stacked mixing assembly is connected to the outlet channel. S3. The vertically stacked mixing component consisting of an inlet integrated plate, several circumferential mixing integrated plates, and an outlet integrated plate are sequentially connected and integrated into a single structure.

10. A method for manufacturing an application structure for the three-dimensional fluid mixer of claim 6, characterized in that: The circumferential hybrid integrated disks are manufactured using methods including 3D printing, machining, etching, photolithography, and injection molding. Each circumferential hybrid integrated disk is assembled into a vertically stacked hybrid component using a stacking assembly method. The application structure is used for fluid mixing, particle mixing, and / or LNP synthesis in fields including biology, medicine, chemicals, food, beverages, and beauty.

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