Method for improving breeding efficiency of cows

Abstract

The invention relates to the technical field of livestock breeding, and provides a method for improving the breeding efficiency of cows, which comprises the following steps: 1, gene screening: a, double-fetal gene screening, and b, follicular reserve detection; step 2, hormone regulation and control: a microneedle patch technology is adopted for drug administration to cows, and a patch contains a PMSG / HCG slow release layer; step 3, intelligent monitoring: a necklace type detector is matched with a vagina temperature sensor to monitor the state of the cow, the data transmission time interval is once every 2 minutes, and whether the cow enters an oestrus stage or not is monitored; 4, precise intervention and embryo optimization: carrying out vitrification on the embryo by using an EG + DMSO protective agent combination, and carrying out microscopic embryo segmentation in a 16-32 cell period; 5, artificial insemination, wherein the optimized embryo is subjected to insemination through a three-channel transplantation catheter. The method has the following advantages that the double-fetal probability is increased, and ovarian cyst is avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of livestock breeding, in particular to a method for improving the reproductive efficiency of cows. BACKGROUND

[0002] Cows are monovular animals, and their reproductive cycle is relatively long and their reproductive rate is relatively low in a natural state. Therefore, a method for improving the reproductive efficiency of cows, achieving one birth per year and multiple births per birth, is of great significance to improving the efficiency of cow breeding.

[0003] In the prior art, there are mainly two methods for improving the reproductive efficiency of cows: one is artificial insemination (AI) + embryo transfer (ET) technology, and the other is an exogenous hormone induction method: using gonadotropins such as pregnant mare serum (PMSG), human chorionic gonadotropin (HCG), three-hormone (ITC), and anti-blood gonadotropin serum (anti-PMSG) to induce cows to produce twins.

[0004] Therefore, there are the following disadvantages: the AI+ET technology has a low embryo implantation rate and cannot guarantee multiple births of cows, and the hormone induction method has a low twin rate and a high risk of ovarian cysts. SUMMARY

[0005] The technical problem to be solved by the present application is the low embryo implantation rate of AI+ET technology and the low twin rate and ovarian cyst risk of the hormone induction method.

[0006] To solve the above technical problems, the technical solution provided by the present application is as follows: a method for improving the reproductive efficiency of cows, comprising the following steps:

[0007] Step 1: genetic screening

[0008] a. Twin gene screening: detecting the mutation sites AQP8 and BMPR1B of the bovine FecB gene;

[0009] b. Follicle reserve detection: using follicle reserve detection AMH rapid test paper to analyze and detect 23 SNP site PCR, evaluating ovarian function, and the detection time is 5 minutes;

[0010] Step 2: hormone regulation

[0011] Using a microneedle patch to administer drugs to the cow, the patch containing a PMSG / HCG sustained-release layer for 72 hours of continuous administration;

[0012] Step 3: intelligent monitoring

[0013] Using a collar detector combined with a vaginal temperature sensor to monitor the state of the cow, the data transmission time interval is 2 minutes once, and whether the cow enters the estrus stage is monitored;

[0014] Step four: precise intervention

[0015] Embryo optimization: vitrification of embryos using EG+DMSO protective agent combination, micro-embryo splitting at 16-32 cell stage;

[0016] Step five: artificial insemination

[0017] Optimized embryos are inseminated using a three-channel implantation catheter.

[0018] Further, the twin gene screening in step one uses SNP chip detection reagent kit for detection.

[0019] Further, in step two: the dosage = the base amount x (1+0.2x BMI+0.15x fetal coefficient).

[0020] Further, after step two, β-cyclodextrin-embedded degradation agent is used to degrade PMSG, and to remove hormone residues.

[0021] Further, 48 hours after step five implantation, specific audio is played, with a frequency range of 125-250 Hz, for preventing stress abortion.

[0022] The present application has the following advantages:

[0023] 1. The FecB gene mutation site detection screening of twin potential individuals, combined with AMH test paper rapid evaluation of ovarian function, can effectively avoid the low efficiency problem caused by genetic factors in traditional breeding;

[0024] 2. The technology of microneedle patch slow-release PMSG / HCG avoids the risk of ovarian cysts caused by traditional hormone injection. DETAILED DESCRIPTION

[0025] The present application will be further described in detail below in conjunction with examples.

[0026] Example 1

[0027] A method for improving the reproductive efficiency of cows, comprising the following steps:

[0028] Step one: genetic screening

[0029] a. Twin gene screening: using SNP chip reagent kit to detect the mutation sites AQP8 and BMPR1B of bovine FecB gene, and when the mutation frequency of AQP8 site is ≥0.7, it is determined as a high reproductive potential individual;

[0030] b.Follicle reserve detection: use follicle reserve detection AMH rapid test paper to analyze and detect 23 SNP site PCR, evaluate ovarian function, detection time is 5 min, AMH≥4.5ng / mL is high-quality ovarian reserve;

[0031] Its operation process is: tail vein blood sampling→test paper infiltration→smart card reader analysis;

[0032] Step two: hormone regulation

[0033] The micro-needle patch technology is used for drug administration to cows, the micro-needle patch needs to be stored at-20℃ in the dark, and the patch contains a PMSG / HCG slow-release layer. The patch structure is a three-layer composite film, which is an isolation layer, a PMSG-HCG slow-release layer, and an adhesive layer, for 72 hours of continuous drug administration.

[0034] Among them, the dosage = basic amount x (1+0.2x BMI+0.15x parity coefficient);

[0035] Further, after hormone regulation, β-cyclodextrin-embedded degrading agent is used to degrade PMSG to remove hormone residues. The concentration of β-cyclodextrin-embedded degrading agent is 12mmol / L, and the residual hormones are removed within 6 hours.

[0036] Step three: intelligent monitoring

[0037] The collar detector is used in combination with a vaginal temperature sensor to monitor the state of the cow, with a data transmission interval of 2 minutes once, to monitor whether the cow enters the estrus stage.

[0038] Step four: precise intervention

[0039] Embryo optimization: use EG+DMSO protectant combination to vitrify the embryo, and perform microscopic embryo splitting at the 16-32 cell stage.

[0040] Step five: artificial insemination

[0041] The optimized embryo is used to complete insemination using a three-channel implantation catheter.

[0042] Further, 48 hours after implantation, specific audio is played, with a frequency range of 125-250Hz, to prevent stress abortion, and the audio intervention needs to avoid the feeding period.

[0043] In specific implementation, the twin potential individual is screened through the FecB gene mutation site detection, and the ovary function is quickly evaluated by combining the AMH test paper, so that the problem of low efficiency caused by genetic factors in traditional breeding can be effectively avoided, and the detection of 23 SNP sites by PCR technology significantly improves the screening efficiency; the technology of the microneedle patch slow release PMSG / HCG avoids the risk of ovarian cyst caused by traditional hormone injection, and 72 hours of continuous drug delivery is more in line with the physiological rhythm of the cow.

[0044] Although the present application has been described in detail with general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of protection required by the present application.

Claims

1. A method of improving the reproductive efficiency of a cow, characterized by: It includes the following steps: Step one: genetic screening a. Twin genetic screening: detect the mutation sites AQP8 and BMPR1B of the bovine FecB gene; b. Follicle reserve detection: use the AMH rapid detection test paper to analyze and detect the PCR of 23 SNP sites, evaluate the ovarian function, and the detection time is 5 minutes; Step two: hormone regulation Use microneedle patch technology to give medicine to the cow, the patch contains PMSG / HCG sustained-release layer, and give medicine for 72 hours continuously; Step three: intelligent monitoring Use the collar detector combined with the vaginal temperature sensor to monitor the state of the cow, the data transmission interval is 2 minutes once, and monitor whether the cow enters the estrus stage; Step four: precise intervention Embryo optimization: use EG+DMSO protectant combination to vitrify the embryo, and perform microscopic embryo splitting at 16-32 cell stage; Step five: artificial insemination Use the three-channel implantation catheter to complete the insemination of the optimized embryo.

2. A method of improving the reproductive efficiency of a cow as claimed in claim 1, wherein: The twin genetic screening in step one is detected by SNP chip detection kit.

3. A method of improving reproductive efficiency in a cow as claimed in claim 1, wherein: In step two: the dose = the basic amount × (1+0.2×BMI+0.15×parity coefficient).

4. A method of improving reproductive efficiency in a cow as claimed in claim 1, wherein: After step two, use β-cyclodextrin to embed the degradation agent to degrade PMSG and remove hormone residues.

5. A method of improving reproductive efficiency in a cow as claimed in claim 1, wherein: Play specific audio 48 hours after step five implantation, the audio frequency range is 125-250Hz, which is used to prevent stress abortion.