Application of octuple hyaluronic acid-containing synthetic raw material composition capable of improving sensitive skin in field of biological application

Abstract

The invention discloses application of a synthetic raw material composition containing octuple hyaluronic acid and capable of improving sensitive skin in the field of biological application. The synthetic raw material composition capable of improving sensitive skin comprises the following raw material components: a sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethyl silanol hyaluronate, hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate and hydrolyzed hyaluronic acid, and is prepared by mixing the raw material components according to a certain formula proportion. The synthetic raw material composition containing the octuple hyaluronic acid and capable of improving the sensitive skin is added into a skin care product, and the adding amount accounts for 1%-10% of the total weight of the skin care product; other materials are conventional auxiliary materials in the field of skin care products; the skin care product added with the synthetic raw material composition can deeply repair the skin, stimulate the vitality of the skin, improve redness, solve various problems of the skin for a long time and enhance the resistance of the skin to the environment, and is suitable for moisturizing and repairing various kinds of skin and skin with damaged barriers.

Description

TECHNICAL FIELD

[0001] The present application relates to a skin care composition, belonging to the technical field of daily chemical products, in particular to the application of a synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin in skin care products. BACKGROUND

[0002] Hyaluronic acid (HA) is one of the most recognized star ingredients, and it is the first and most widely recognized champion in terms of moisturizing.

[0003] The effects of hyaluronic acid on the skin include the following aspects: skin physical barrier: hyaluronic acid combines with protein to form a gel-like structure of HA-protein-water, which has water retention, lubricity, film formation, promotion of epithelial regeneration, safety, etc. Antioxidant of skin: preventing the production of enzymes related to the formation of free radicals in cells, reducing the formation of free radicals, and preventing lipid peroxidation to cause aging of the body. Moisturizing effect of sebum film: it has good film forming property, can form a uniform film on the skin surface, reduce the evaporation of water on the skin surface, and thus provide a good moisturizing environment for the skin. Skin wound healing: it can regulate collagen synthesis and promote wound healing, providing a favorable repair environment for the repair of sensitive skin barrier and wound healing.

[0004] The skin barrier is mainly composed of sebum film and stratum corneum. It mainly plays the role of maintaining skin moisture, preventing body water evaporation, and resisting foreign matter invasion from the outside. Excessive and frequent force stripping and removal are equivalent to "forcible demolition" of the skin's natural barrier, which can easily cause skin itching, sensitivity and inflammation.

[0005] However, the existing technology still has few studies on the effect of low molecular weight hyaluronic acid complex composition on sensitive skin. SUMMARY

[0006] In order to overcome the defects existing in the prior art, the purpose of the present application is to provide the application of a synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin in skin care products, which screens 8 kinds of large, medium, small and micro molecules of HA through molecular weight interval control technology, has a honeycomb network type three-dimensional penetration, releases small molecule active ingredients, deeply repairs the skin, stimulates the skin's own vitality, improves redness, and solves various problems of the skin.

[0007] The purpose of the present application and the solution to its technical problems are realized by using the following technical scheme. According to the present application, a synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin is added to skin care products, and the addition amount is 1%-10% of the total weight of the skin care product; other is conventional auxiliary material in the field of skin care products;

[0008] The synthetic raw material composition is composed of the following raw material components by weight:

[0009] Water 68-92 parts, 1,2-pentanediol 0.5-5 parts, 1,2-hexanediol 0.5-5 parts, sodium hyaluronate 0.5-1.5 parts, potassium hyaluronate 0.5-1.5 parts, hyaluronic acid 1-5 parts, hydrolyzed hyaluronic acid 2-5 parts, hydrolyzed sodium hyaluronate 0.5-1.5 parts, acetylated sodium hyaluronate 1-3 parts, sodium hyaluronate cross-linked polymer 1-3 parts, dimethylsilyl hyaluronate 0.5-1.5 parts.

[0010] Further, the application of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in skin care products, the synthetic raw material composition is composed of the following raw material components by weight:

[0011] Water 75-65 parts, 1,2-pentanediol 2-4 parts, 1,2-hexanediol 1-3 parts, sodium hyaluronate 0.8-1.2 parts, potassium hyaluronate 0.8-1.2 parts, hyaluronic acid 2-4 parts, hydrolyzed hyaluronic acid 3-5 parts, hydrolyzed sodium hyaluronate 0.8-1.2 parts, acetylated sodium hyaluronate 1-3 parts, sodium hyaluronate cross-linked polymer 1-3 parts, dimethylsilyl hyaluronate 0.8-1.2 parts.

[0012] Further, the application of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in skin care products, the synthetic raw material composition is composed of the following raw material components by weight:

[0013] Water 80 parts, 1,2-pentanediol 3 parts, 1,2-hexanediol 2 parts, sodium hyaluronate 1 part, potassium hyaluronate 1 part, hyaluronic acid 3 parts, hydrolyzed hyaluronic acid 4 parts, hydrolyzed sodium hyaluronate 1 part, acetylated sodium hyaluronate 2 parts, sodium hyaluronate cross-linked polymer 2 parts, dimethylsilyl hyaluronate 1 part.

[0014] The application provides the application of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in skin care products, the synthetic raw material composition is prepared by the following process steps:

[0015] 1) Stir and mix 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethylsilyl hyaluronate, and water for 10-15 min, and heat while stirring to obtain mixture A.

[0016] 2) Add hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, and hydrolyzed hyaluronic acid into mixture A in turn while stirring, and stir and mix for 10-15 min.

[0017] 3) homogenize for 3-5 min, cool to room temperature, to obtain a skin care composition containing octaplex hyaluronic acid that can improve sensitive skin.

[0018] Further, in step 1), the heating temperature is 40-45℃.

[0019] Further, in step 3), the homogenization speed is 50-1000r.

[0020] In addition, the application of the synthetic raw material composition containing octaplex hyaluronic acid that can improve sensitive skin in skin care products, the skin care product is an emulsion containing 5% octaplex hyaluronic acid skin care composition, and the preparation process is as follows:

[0021] A phase: sucrose poly-cottonseed oil acid ester 4 parts, tocopherol acetate 1.5 parts, thyme essential oil 0.5 parts;

[0022] B phase: glycerol 10 parts;

[0023] C phase: butanediol 5 parts, oat beta-glucan 0.05 parts, betaine 2 parts, baicalin 0.2 parts, sophoramine 0.1 parts, salicin 0.3 parts, p-hydroxyacetophenone 0.4 parts, 1,2-hexanediol 0.4 parts, example 1 octaplex hyaluronic acid skin care composition 5 parts, deionized water balance.

[0024] Add the components of A phase together into the reaction pot, stir at 30r / m until completely dissolved, then add the components of B phase, heat to 40-45℃ and keep for about 20 minutes, stir at 30r / m until completely dissolved, then add the components of C phase and stir (30r / m) until completely dissolved, and then cool to room temperature to obtain an octaplex hyaluronic acid emulsion.

[0025] In addition, the application of the synthetic raw material composition containing octaplex hyaluronic acid that can improve sensitive skin in skin care products, the skin care product is an emulsion containing 5% octaplex hyaluronic acid skin care composition, and the preparation process is as follows:

[0026] A component: PEG-40 hydrogenated castor oil 1 part, methyl polytrimethylsiloxane 5 parts, di-octyl carbonate 4 parts;

[0027] B component: glycerol 3 parts, 82.1% water;

[0028] C component: example 1 octaplex hyaluronic acid skin care composition 5 parts, multi-skin containing palmitoyl tri-skin-1 and palmitoyl tetra-skin-7 1 part, glycosphingolipid 1 part.

[0029] (1) Stir the A component at 80rpm to 40-45℃, keep for 10min, and obtain an oil phase raw material;

[0030] (2) Put the B component in a container, stir at 60 rpm, and heat to 40-45℃ for 20 min to prepare a water phase raw material;

[0031] (3) Put the B component raw material into an emulsifying homogenizing device, start stirring, and slowly suck the A component raw material into the emulsifying device at 40-45℃ to obtain a mixed liquid; start homogenizing for 30 s at 5000 rpm;

[0032] (4) After homogenization, keep the stirring speed unchanged, cool to 40℃, add the C component, stir for 15 min, and discharge to obtain an octuple hyaluronic acid essence.

[0033] Further, the application provides use of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in skin care products, wherein the addition amount of the synthetic raw material composition in the skin care product is 5%, the skin care product is a product in the form of water, cream, emulsion, or facial mask, and the conventional auxiliary material is a mixture of one or more of emulsifiers, surfactants, emollients, solubilizers, thickening agents, skin conditioning agents, humectants, pH regulators, preservatives, chelating agents, fragrances, and deionized water.

[0034] By means of the above technical scheme, the skin care product composition containing octuple hyaluronic acid for improving sensitive skin has the following advantages:

[0035] 1) Full molecular weight stratified penetration; improving rough skin, reducing water loss, increasing water content in the stratum corneum, promoting the generation of natural moisturizing factors, activating aquaporin, repairing the barrier function, and improving skin tolerance;

[0036] 2) Stereoscopic efficacy matrix closed loop; converging octuple hyaluronic acid to form a multi-dimensional hyaluronic acid magic cube;

[0037] 3) Strong repair, synergistic moisturizing and antioxidant; resisting cell damage caused by various stimuli, reducing the occurrence of skin inflammation; resisting ultraviolet rays and chemical damage, promoting the repair of the skin barrier, resisting the external environment, and establishing secondary protection;

[0038] 4) Small molecular particle size penetration of the dermis; instantaneous water replenishment, long-lasting water retention, and long-acting water;

[0039] 5) High active content, high efficiency, and synergistic effect; inhibiting the release of inflammatory factors, reducing facial redness, and improving sensitive skin; water locking force in the dermis and nourishing force on the surface;

[0040] 6) High stability, safety, solubility, and compatibility. BRIEF DESCRIPTION OF DRAWINGS

[0041] Figure 1 It is a unique enzyme cutting technology for obtaining a target hyaluronic acid fragment to accurately cut a large molecular hyaluronic acid.

[0042] Figure 2 is a molecular weight (Da) distribution profile of different molecular weight HA;

[0043] Figure 3 is a selectivity profile of low molecular weight HA;

[0044] Figure 4a shows a Raman spectrum of the octapeptide HA-containing skin care composition of Example 1 - depth of penetration into skin;

[0045] Figure 4b shows a Raman spectrum of standard HA - depth of penetration into skin;

[0046] Figure 5a shows a plot of the effect of volatilization on skin water content over time between the octapeptide HA-containing emulsion group, the multi-HA emulsion group, and the blank control group of Application Example 1;

[0047] Figure 5b shows a plot of the effect of volatilization on skin water loss over time between the octapeptide HA-containing emulsion group, the multi-HA emulsion group, and the blank control group of Application Example 1.

[0048] Figure 6a shows a plot of the effect of volatilization on skin water content over time between the octapeptide HA-containing emulsion group, the multi-HA emulsion group, and the blank control group of Application Example 1;

[0049] Figure 6b shows a plot of the effect of volatilization on TEWL values over time between the octapeptide HA-containing emulsion group, the multi-HA emulsion group, and the blank control group of Application Example 1.

[0050] Figure 7a shows a bar graph of the degranulation rate;

[0051] Figure 7b shows a morphological observation of mast cell degranulation.

[0052] Figure 8a shows a plot of NF-κB expression at different concentrations;

[0053] Figure 8b shows a plot of the contents of inflammatory factors IL-1α and TNF-α.

[0054] Figure 9 shows a schematic diagram of morphological changes in the model tissue.

[0055] Figure 10a shows a plot of the changes in the content of protein FLG (filaggrin);

[0056] Figure 10b Figure 7 shows the graph of the change in the content of LOR protein (comucolin).

[0057] Figure 10c Figure 8 shows the graph of the change in the content of CD44 protein (type I transmembrane glycoprotein).

[0058] Figure 11 Figure 9 shows the column graph of the barrier-related protein.

[0059] Figure 12a Figure 10 shows the graph of the change in the scavenging rate of superoxide anion radicals by the skin care composition containing octet hyaluronic acid at different amounts of addition. Figure 12b Figure 11 shows the graph of the change in the scavenging rate of hydroxyl radicals by the skin care composition containing octet hyaluronic acid at different amounts of addition. Figure 12c Figure 12 shows the graph of the change in the scavenging rate of DPPH radicals by the skin care composition containing octet hyaluronic acid at different amounts of addition.

[0060] Figure 13 Figure 13 is a facial effect graph of a volunteer using the 5% octet hyaluronic acid-containing serum of the present application;

[0061] Figure 14 Figure 14 is a facial inflammation graph of a volunteer 14 days after using the 5% octet hyaluronic acid-containing serum;

[0062] Figure 15 Figure 15 is a facial graph of a volunteer using the 5% octet hyaluronic acid-containing serum;

[0063] Figure 16 Figure 16 is a graph of the skin redness condition after 24 hours of using the skin care composition containing octet hyaluronic acid;

[0064] Figure 17 Figure 17 is a graph of the skin condition after 7 days of using the skin care composition containing octet hyaluronic acid; DETAILED DESCRIPTION

[0065] The present application discloses a skin care composition containing octet hyaluronic acid for improving sensitive skin and a preparation method thereof. The raw material components of the skin care composition for improving sensitive skin include sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethylsilanol hyaluronate, hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, and hydrolyzed hyaluronic acid, which are mixed in a certain formula ratio.

[0066] Specifically, the following components are included:

[0067] Water 68-92 parts, 1,2-pentanediol 0.5-5 parts, 1,2-hexanediol 0.5-5 parts, sodium hyaluronate 0.5-1.5 parts, potassium hyaluronate 0.5-1.5 parts, hyaluronic acid 1-5 parts, hydrolyzed hyaluronic acid 2-5 parts, hydrolyzed sodium hyaluronate 0.5-1.5 parts, acetylated sodium hyaluronate 1-3 parts, sodium hyaluronate cross-linked polymer 1-3 parts, dimethylsilyl hyaluronate 0.5-1.5 parts.

[0068] The present application is a three-dimensional matrix containing eight different hyaluronic acids.

[0069] The high-polymer sodium hyaluronate is a material with super-high biological activity obtained by double enzyme cutting technology, cross-linking technology and spray drying process, and smaller complete ultra-small HA is obtained, and the molecular weight is as low as 3000 Dalton, and the repair effect is remarkable.

[0070] Eight kinds of HA with different molecular weights are screened by molecular weight interval control technology, and the HA is in a honeycomb network type three-dimensional penetration, the active ingredients of small molecules are released, the skin is deeply repaired, the skin activity is stimulated, the redness is improved, the various problems of the skin are solved for a long time, the resistance of the skin to the environment is enhanced, and the moisturizing and repair of various skin and barrier damaged skin are suitable.

[0071] As shown in Figure 1 The present application adopts double enzyme cutting technology, uses two restriction enzymes to cut the two ends of the target fragment respectively, and then uses a ligase to connect, so as to obtain the target hyaluronic acid fragment.

[0072] As shown in Figure 2 The unique enzyme cutting technology of the present application accurately cuts the large molecular weight hyaluronic acid, and the molecular weight is as low as 3000 Da.

[0073] Advantages of double enzyme cutting technology:

[0074] 1) The molecular weight is accurately controlled;

[0075] 2) The types of fragments obtained by double enzyme cutting are reduced, the difficulty of screening is reduced, and it is simpler to remove non-target products.

[0076] As shown in Figure 3 The selection of low molecular weight hyaluronic acid, HA has remarkable repair effect, the lower the molecular weight, the more obvious the effect. The present application utilizes double enzyme cutting technology, cross-linking technology and spray drying process to obtain a material with super-high biological activity, and smaller complete ultra-small HA is obtained, and the molecular weight is as low as 3000 Dalton, and the repair effect is remarkable.

[0077] The eight-hydrated hyaluronic acid skin care product composition of the present application adopts the following preparation method:

[0078] 1) 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethylsilanol hyaluronate, water are stirred and mixed for 10-15 min, heated to 40-45 DEG C while stirring, to obtain mixture A.

[0079] 2) hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, hydrolyzed hyaluronic acid are sequentially added to mixture A while stirring, and stirred and mixed for 10-15 min.

[0080] 3) homogenized at 5000-10000 r for 3-5 min, cooled to room temperature, to obtain an octa-hyaluronic acid-containing skin care product composition capable of improving sensitive skin.

[0081] The modified active further enhances the affinity with the skin and increases the skin permeability of the active. It is water-soluble, preservative-free, EO-free, and ethanol-free; and is suitable for various dosage forms such as water, essence, mask, cream, and emulsion.

[0082] It is recommended to add the product in the low-temperature stage of the formula, control the temperature at 45-50 DEG C, and shear at low speed to stir uniformly.

[0083] The present application is a three-dimensional matrix containing the following eight kinds of hyaluronic acid, which has layered precise action, time-release penetration, skin revitalization and protection, and farewell to delicate skin. It has three-dimensional moisturizing, soothing anti-inflammatory, water-locking repair, and enhanced barrier protection. The effects of the eight kinds of hyaluronic acid raw materials are as follows:

[0084] ① Sodium hyaluronate

[0085] Natural moisturizing factor, hydrates the stratum corneum, forms the outer membrane of the skin, moisturizes the skin, and reduces water loss in the stratum corneum.

[0086] ② Potassium hyaluronate

[0087] Supplementing trace elements of the skin, repairing and anti-inflammatory; attracting internal skin moisture to the skin surface, keeping the skin moist and elastic.

[0088] ③ Hyaluronic acid

[0089] Deep moisturizing, removing free radicals; promoting the synthesis of hyaluronidase and hyaluronic acid; preventing skin peroxidation and delaying aging.

[0090] ④ Hydrolyzed sodium hyaluronate

[0091] Relieving inflammation caused by ultraviolet rays, chemicals, and microbial pollution, relieving skin sensitivity; repairing skin cell damage caused by ultraviolet or chemical stimulation.

[0092] ⑤ Acetylated sodium hyaluronate

[0093] Hydrophilic and lipophilic, embedded in the skin, double moisturizing; antioxidant, anti-inflammatory, repair the skin barrier; improve skin elasticity and other biological activity functions.

[0094] Hydrolyzed hyaluronic acid

[0095] Deep into the skin to increase the internal moisture of the skin, supplement the water of cells and cell matrix; relieve collagen loss and maintain skin moisture.

[0096] Dimethyl silanol hyaluronate

[0097] Moisturizing while eliminating stickiness. Prevent and repair skin damage.

[0098] Sodium hyaluronate cross-linked polymer

[0099] Form a dense three-dimensional network structure, evenly cover the skin surface; enhance the skin barrier function, isolate pollution, resist UV radiation.

[0100] Table 1 below is the formulation of the skin care compositions of Examples 1-3 and Comparative Examples 1-4.

[0101] Table 1 Formulation

[0102]

[0103] Example 1

[0104] A synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin is made from the following raw material components by weight:

[0105] Water 80 parts, 1,2-pentanediol 3 parts, 1,2-hexanediol 2 parts, sodium hyaluronate 1 part, potassium hyaluronate 1 part, hyaluronic acid 3 parts, hydrolyzed hyaluronic acid 4 parts, hydrolyzed sodium hyaluronate 1 part, acetylated sodium hyaluronate 2 parts, sodium hyaluronate cross-linked polymer 2 parts, dimethyl silanol hyaluronate 1 part.

[0106] 1) Stir and mix 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethyl silanol hyaluronate, and water for 10 min, and heat to 40°C while stirring to obtain mixture A.

[0107] 2) Add hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, and hydrolyzed hyaluronic acid to mixture A in turn while stirring, and stir and mix for 10 min.

[0108] 3) Homogenize at 5000 r for 3 min, and cool to room temperature to obtain a skin care composition containing eight hyaluronic acids for improving sensitive skin.

[0109] Example 2

[0110] A synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin is prepared from the following raw material components by weight:

[0111] Water 92 parts, 1,2-pentanediol 0.5 parts, 1,2-hexanediol 0.5 parts, sodium hyaluronate 0.5 parts, potassium hyaluronate 0.5 parts, hyaluronic acid 1 part, hydrolyzed hyaluronic acid 2 parts, hydrolyzed sodium hyaluronate 0.5 parts, acetylated sodium hyaluronate 1 part, sodium hyaluronate cross-linked polymer 1 part, dimethylsilyl hyaluronate 0.5 parts.

[0112] 1) 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethylsilyl hyaluronate, and water are mixed by stirring for 10 minutes, and heated to 40°C while stirring, to obtain a mixture A.

[0113] 2) Hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, and hydrolyzed hyaluronic acid are sequentially added to the mixture A while stirring, and mixed by stirring for 10 minutes.

[0114] 3) Homogenized at 5000 r for 3 minutes, and cooled to room temperature, to obtain a synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin.

[0115] Example 3

[0116] A synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin is prepared from the following raw material components by weight:

[0117] Water 68 parts, 1,2-pentanediol 5 parts, 1,2-hexanediol 5 parts, sodium hyaluronate 1.5 parts, potassium hyaluronate 1.5 parts, hyaluronic acid 5 parts, hydrolyzed hyaluronic acid 5 parts, hydrolyzed sodium hyaluronate 1.5 parts, acetylated sodium hyaluronate 3 parts, sodium hyaluronate cross-linked polymer 3 parts, dimethylsilyl hyaluronate 1.5 parts.

[0118] 1) 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethylsilyl hyaluronate, and water are mixed by stirring for 15 minutes, and heated to 45°C while stirring, to obtain a mixture A.

[0119] 2) Hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, and hydrolyzed hyaluronic acid are sequentially added to the mixture A while stirring, and mixed by stirring for 15 minutes.

[0120] 3) Homogenized at 5000-10000 r for 5 minutes, and cooled to room temperature, to obtain a synthetic raw material composition containing eight hyaluronic acids for improving sensitive skin.

[0121] Comparative Example 1

[0122] The formulation is shown in Table 1. The preparation method is as follows: except that the sodium hyaluronate is not added, the rest is carried out according to the process of Example 1.

[0123] Comparative Example 2

[0124] The formulation is shown in Table 1. The preparation method is as follows: except that the sodium hyaluronate is not added, the rest is carried out according to the process of Example 1.

[0125] Comparative Example 3

[0126] The formulation is shown in Table 1. The preparation method is as follows: except that the sodium hyaluronate is not added, the rest is carried out according to the process of Example 1.

[0127] Comparative Example 4

[0128] The formulation is shown in Table 1. The preparation method is as follows: except that the sodium hyaluronate is not added, the rest is carried out according to the process of Example 1.

[0129] Application Example 1

[0130] The skin care emulsion of the synthetic raw material composition containing 5% octa-hyaluronate is prepared as follows:

[0131] Phase A: sucrose polycottonseed oil acid ester 4 parts, tocopheryl acetate 1.5 parts, thyme essential oil 0.5 parts;

[0132] Phase B: glycerol 10 parts;

[0133] Phase C: butanediol 5 parts, oat beta-glucan 0.05 parts, betaine 2 parts, baicalin 0.2 parts, sophoramine 0.1 parts, salicin 0.3 parts, p-hydroxyacetophenone 0.4 parts, 1,2-hexanediol 0.4 parts, Example 1 octa-hyaluronate-containing skin care composition 5 parts, deionized water balance.

[0134] The components of phase A are added together into a reaction kettle, stirred at 30 r / m until completely dissolved, then the components of phase B are added, heated to 40-45°C and kept for about 20 minutes, then the components of phase C are added and stirred (30 r / m) until completely dissolved, and then cooled to room temperature to obtain an octa-hyaluronate-containing emulsion.

[0135] Application Example 2

[0136] The skin care serum of the synthetic raw material composition containing 5% octa-hyaluronate is prepared as follows:

[0137] A component: PEG-40 hydrogenated jojoba 1 part, methyl polysilicone 5 parts, dioctyl carbonate 4 parts;

[0138] B component: glycerin 3 parts, 82.1% water;

[0139] C component: Example 1 eightfold hyaluronic acid-containing skin care product composition 5 parts, skin-1 and skin-7 containing palmitoyl tetrahyaluronate 1 part, sugar sphingolipid 1 part.

[0140] (1) The A component was stirred at 80 rpm and warmed to 40-45°C for 10 min, and then kept at 40-45°C for 10 min to obtain the oil phase raw material;

[0141] (2) The B component was placed in a container, stirred at 60 rpm, and warmed to 40-45°C for 20 min, and then kept at 40-45°C for 20 min to obtain the water phase raw material;

[0142] (3) The B component raw material was added to the emulsification homogenization device, and the stirring was started. The A component raw material was slowly sucked into the emulsification device at 40-45°C to obtain a mixed solution. The homogenization was started for 30 s at 5000 rpm;

[0143] (4) After homogenization, the stirring speed was kept unchanged, and the temperature was cooled to 40°C. The C component was added, and stirred for 15 min. The product was discharged to obtain the eightfold hyaluronic acid-containing essence.

[0144] The following are the contents of the efficacy test examples.

[0145] Efficacy Test Example

[0146] I. In vitro efficacy test

[0147] 1. Skin permeability test (Raman spectrum-penetrating skin depth)

[0148] Raman can penetrate the skin layer with transdermal preparations. The active ingredients in the transdermal preparation need to pass through the skin barrier to subcutaneous or blood. When administered on the skin surface, the drug enters the body circulation at a constant rate (or close to a constant rate) through the skin to produce systemic or local therapeutic effect. Raman spectrum can penetrate the skin layer to obtain real-time drug distribution in the skin. The experimental method is the same as the conventional technology.

[0149] Figure 4a The Raman spectrum-penetrating skin depth display of the eightfold hyaluronic acid-containing skin care product composition of Example 1 is shown;

[0150] Figure 4b The Raman spectrum-penetrating skin depth display of the standard hyaluronic acid is shown.

[0151] Conclusion: As Figure 4aAs shown in the b contrast, the results show that the eightfold hyaluronic acid-containing skin care product composition of Example 1 can penetrate the skin deeper than the standard hyaluronic acid and store in different skin layers.

[0152] 2. Short-term moisturizing efficacy test

[0153] Moisturizing cosmetics mainly play a moisturizing role on the skin through moisture absorption and water retention. Moisture absorption is that the moisturizing agents (such as glycerol, hyaluronic acid, oat polypeptide, etc.) in the cosmetics absorb water molecules in the air to keep the stratum corneum of the skin moist; water retention is that the cosmetics form a closed lubricating film on the skin surface to limit the loss of water on the skin surface in the environment. The experimental method is the same as the conventional technology.

[0154] Figure 5a The figure shows the volatilization effect of skin water content over time between the eightfold hyaluronic acid-containing emulsion group, the multiple HA emulsion group and the blank control group of Application Example 1;

[0155] Figure 5b The figure shows the volatilization effect of skin water loss over time between the eightfold hyaluronic acid-containing emulsion group, the multiple HA emulsion group and the blank control group of Application Example 1.

[0156] As shown in Figure 5, the eightfold hyaluronic acid-containing skin care product composition of the application can instantly play a moisturizing role, continuously supplement water to the skin, improve the internal water accumulation of the skin, and make the skin full of water and revive, soft and elastic. Compared with 0h, the skin water content increased by 37% at 8h, and the skin water loss decreased by 27%.

[0157] 3. Long-term moisturizing efficacy test

[0158] Although the trans-epidermal water loss cannot directly reflect the water content of the stratum corneum, the trans-epidermal water loss value is an important parameter of the barrier function of the stratum corneum. The higher the trans-epidermal water loss value, the more water is lost, and the worse the barrier function of the stratum corneum. The experimental method is the same as the conventional technology.

[0159] Figure 6a The figure shows the volatilization effect of skin water content over time between the eightfold hyaluronic acid-containing emulsion group, the multiple HA emulsion group and the blank control group of Application Example 1;

[0160] Figure 6b The figure shows the TEWL value change over time between the eightfold hyaluronic acid-containing emulsion group, the multiple HA emulsion group and the blank control group of Application Example 1.

[0161] As shown in Figure 6, the skin moisture content of the 5% octasodium hyaluronate emulsion of Example 1 of the present application was 12% higher than that of the blank emulsion group after 28 days of continuous use, and the difference between the groups was significant. The amount of transdermal water loss was lower than that of the blank emulsion group during the 28 days of use, and in particular, the amount of transdermal water loss of the octasodium hyaluronate emulsion group was 15% lower than that of the blank emulsion group after 14 days of use.

[0162] 4. Anti-allergic efficacy test

[0163] The anti-allergic efficacy of the sample was evaluated by morphological observation of mast cell degranulation and degranulation rate using a C48 / 80 stimulated mast cell model.

[0164] Figure 7a A bar chart showing the degranulation rate is shown.

[0165] Figure 7b A morphological observation chart of mast cell degranulation is shown.

[0166] The results of Figure 7 show that the mast cell degranulation rate of the 1% octasodium hyaluronate skin care product composition group of Example 1 decreased significantly compared to the NC group, with a decrease of 25%, indicating anti-allergic efficacy.

[0167] 5. Anti-inflammatory efficacy test

[0168] An in vitro model of skin inflammatory damage was established by stimulating keratinocytes with UVB, and the anti-inflammatory efficacy of the sample was evaluated by detecting the expression of NF-κB and the expression of inflammatory factors TNFα and IL-1α at different concentrations.

[0169] Figure 8a A graph showing the expression of NF-κB at different concentrations is shown.

[0170] Figure 8b A statistical chart showing the content of inflammatory factors IL-1α and TNF-α is shown.

[0171] The results of Figure 8 show that the expression of NF-κB in the octasodium hyaluronate skin care product composition group decreased significantly, and the inflammatory factor IL-1α decreased by 50% compared to the NC group, and the average concentration of TNF-α decreased significantly, with a decrease of 17%, indicating anti-inflammatory efficacy.

[0172] 6. Barrier damage repair efficacy - histomorphological changes

[0173] A 3D skin model was stimulated with SLS Histomorphological changes were observed;

[0174] Figure 9 A schematic diagram showing the histomorphological changes of the model is shown.

[0175] The sample containing eightfold hyaluronic acid skin care product composition group model four-layer structure boundary is clear, and the damaged condition of living cell layer is significantly improved. The phenomenon of keratin layer loosening and thickening is also significantly alleviated. The sample has repairing effect on the morphological damage of 3D epidermis model caused by SLS.

[0176] 7. Barrier damage repairing effect - barrier-related proteins

[0177] The barrier function of the skin mainly relies on the keratin layer of the epidermis, which is composed of protein-rich keratinocytes and intercellular lipids. Keratinocytes are derived from basal stem cells and synthesize keratin and cornified envelope proteins. Keratin and filaggrin are the main structural proteins in the epidermis, and keratin has a unique structure, thus having specificity in function. The experimental method is the same as the conventional technique.

[0178] Figure 10a The figure shows the change in the content of protein FLG (filaggrin);

[0179] Figure 10b The figure shows the change in the content of LOR protein (loricrin);

[0180] Figure 10c The figure shows the change in the content of CD44 protein (type I transmembrane glycoprotein).

[0181] Figure 11 The figure shows the column chart of barrier-related proteins.

[0182] Compared with the NC group, the contents of FLG (filaggrin), LOR (loricrin), and CD44 protein (type I transmembrane glycoprotein) in the sample increased significantly by 21%, 23%, and 17%, respectively, indicating that the sample has repairing effect on the barrier damage of 3D epidermis model caused by SLS.

[0183] Based on the experimental results, the sample has obvious soothing, anti-inflammatory, and skin barrier repairing effects.

[0184] 8. Antioxidant effect test

[0185] Figure 12a The figure shows the change in the clearance rate of superoxide anion free radicals with different amounts of eightfold hyaluronic acid-containing skin care product composition;

[0186] Figure 12bThe figure shows the change of the scavenging rate of hydroxyl radicals with different amounts of eightfold hyaluronic acid-containing skin care product composition; antioxidant is to scavenge these excess free radicals, or indirectly consume substances prone to generate free radicals, thereby playing a certain anti-aging effect. For example, by detecting the ability of cosmetic raw materials to inhibit cell scavenging of superoxide anion radicals, hydroxyl radicals, and DPPH radicals, etc., the antioxidant effect of the raw material is evaluated, etc. The experimental method is the same as the conventional technology.

[0187] Figure 12c The figure shows the change of the scavenging rate of DPPH radicals with different amounts of eightfold hyaluronic acid-containing skin care product composition.

[0188] As can be seen from Figure 12, in vitro, eightfold hyaluronic acid-containing skin care product composition can effectively scavenge superoxide anion radicals, hydroxyl radicals and DPPH radicals; the above data show that the eightfold hyaluronic acid-containing skin care product composition has good antioxidant performance.

[0189] 9. Safety test - skin patch test

[0190] Test method: refer to the human skin patch test method in Chinese "Cosmetic Safety Technology Standard" 2015 version.

[0191] Testers age: 18-60 years old;

[0192] Number of test subjects: 30;

[0193] Test sample: 5% and 10% eightfold hyaluronic acid-containing skin care product composition aqueous solution

[0194] Table 2 Results of human patch test

[0195]

[0196] Test results: 30 cases of skin closed patch test showed that the eightfold hyaluronic acid-containing skin care product composition is a safe cosmetic raw material.

[0197] II. Clinical human efficacy test

[0198] 1. Clinical test scheme:

[0199] Volunteer: female, 30 years old;

[0200] Test sample: 5% Example 1 essence (application example 2 eightfold hyaluronic acid-containing skin care product composition essence), 5% Example 2, 5% Example 3 essence; 5% Comparative Examples 1-4 essence;

[0201] Method of use: once a day in the morning and evening, evenly applied to the face;

[0202] Trial period: 14 days.

[0203] Test results:

[0204] Table 3 Skin improvement distribution table

[0205] Grouping Effective (human) Effective (human) Ineffective (human) Example 1 10 0 0 Example 2 7 3 0 Example 3 10 0 0 Comparative Example 1 6 4 0 Comparative Example 2 4 6 0 Comparative Example 3 6 4 0 Comparative Example 4 5 5 0

[0206] The improvement effect is shown in Table 3. After the inventive volunteers used the 5% octet hyaluronic acid-containing skin care product composition essence for 14 days, the facial inflammation was improved, and the skin color was visibly improved.

[0207] As shown in Figure 13 , after the inventive volunteers used the 5% octet hyaluronic acid-containing essence for 14 days, the facial inflammation was improved, and the skin color was visibly improved.

[0208] 2. Clinical test two

[0209] Volunteer: female, 35 years old;

[0210] Test sample: application example 2 5% octet hyaluronic acid-containing essence;

[0211] Method of use: once a day in the morning and evening, evenly applied to the face;

[0212] Trial period: 14 days;

[0213] As shown in Figure 14 , the higher the facial brightness, the more inflammatory factors accumulate, and after the volunteers used the 5% octet hyaluronic acid-containing essence for 14 days, the facial inflammation was improved, and the uniformity of the skin color was improved.

[0214] 3. Clinical test three

[0215] Test sample: application example 2 5% octet hyaluronic acid-containing essence;

[0216] Method of use: once a day in the morning and evening, evenly applied to the face;

[0217] Trial period: 14 days;

[0218] As shown in Figure 15 , after the volunteers used the 5% octet hyaluronic acid-containing essence for 14 days, the facial redness was significantly improved.

[0219] 4. Clinical test four (clinical trial - redness and desquamation)

[0220] As shown in Figure 16 , after using the octet hyaluronic acid-containing skin care product composition for 24 hours, the skin redness was significantly improved; after using it for 28 days, 95% of the feedback effective people.

[0221] As shown in Figure 17As shown, after 7 days of using the skin care product composition containing octasaccharide hyaluronic acid, the dry and flaky skin condition is obviously improved; after 28 days of use, the feedback effective number is 100%.

[0222] In summary, the skin care product of the present application is suitable for daily & sensitive skin care; reduces product irritation; promotes barrier protein synthesis, soothes and anti-inflammatory, barrier repair; resists UV damage, repairs damaged skin; the stereoscopic matrix composed of 8 HA and its derivatives precisely acts in layers; water replenishment, water locking, penetration release, deep repair, and healthy skin activation.

[0223] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Any simple modification, equivalent change and modification of the above embodiment without departing from the technical solution content of the present application, according to the technical essence of the present application, all still belong to the scope of the technical solution of the present application.

Claims

1. Use of a synthetic raw material composition containing octasaccharide hyaluronic acid for improving sensitive skin in a skin care product, characterized in that: The synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin is added to the skin care product in an amount of 1-10% of the total weight of the skin care product. The others are conventional auxiliaries in the field of skin care products. The synthetic raw material composition is composed of the following raw material components by weight: water 68-92 parts, 1,2-pentanediol 0.5-5 parts, 1,2-hexanediol 0.5-5 parts, sodium hyaluronate 0.5-1.5 parts, potassium hyaluronate 0.5-1.5 parts, hyaluronic acid 1-5 parts, hydrolyzed hyaluronic acid 2-5 parts, hydrolyzed sodium hyaluronate 0.5-1.5 parts, acetylated sodium hyaluronate 1-3 parts, sodium hyaluronate cross-linked polymer 1-3 parts, dimethylsilyl hyaluronate 0.5-1.5 parts.

2. The use of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in a skin care product according to claim 1, characterized in that: The synthetic raw material composition is composed of the following raw material components by weight: water 75-65 parts, 1,2-pentanediol 2-4 parts, 1,2-hexanediol 1-3 parts, sodium hyaluronate 0.8-1.2 parts, potassium hyaluronate 0.8-1.2 parts, hyaluronic acid 2-4 parts, hydrolyzed hyaluronic acid 3-5 parts, hydrolyzed sodium hyaluronate 0.8-1.2 parts, acetylated sodium hyaluronate 1-3 parts, sodium hyaluronate cross-linked polymer 1-3 parts, dimethylsilyl hyaluronate 0.8-1.2 parts.

3. The use of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in a skin care product according to claim 1, characterized in that: The synthetic raw material composition is composed of the following raw material components by weight: water 80 parts, 1,2-pentanediol 3 parts, 1,2-hexanediol 2 parts, sodium hyaluronate 1 part, potassium hyaluronate 1 part, hyaluronic acid 3 parts, hydrolyzed hyaluronic acid 4 parts, hydrolyzed sodium hyaluronate 1 part, acetylated sodium hyaluronate 2 parts, sodium hyaluronate cross-linked polymer 2 parts, dimethylsilyl hyaluronate 1 part.

4. The use of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in a skin care product according to any one of claims 1-3, characterized in that: The synthetic raw material composition is prepared by the following process steps: 1) Stir and mix 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate cross-linked polymer, sodium hyaluronate, potassium hyaluronate, dimethylsilyl hyaluronate, and water for 10-15 min, and heat while stirring to obtain mixture A. 2) Add hyaluronic acid, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, and hydrolyzed hyaluronic acid to mixture A in sequence while stirring, and stir and mix for 10-15 min. 3) Homogenize for 3-5 min, cool to room temperature, and obtain the octuple hyaluronic acid-containing skin care product composition for improving sensitive skin.

5. The use of the synthetic raw material composition for improving sensitive skin containing octasaccharide hyaluronic acid according to claim 4 in a skin care product, characterized in that, In step 1), the heating temperature is 40-45°C.

6. Use of the synthetic raw material composition for improving sensitive skin containing octasaccharide hyaluronic acid according to claim 4 in a skin care product, characterized in that, In step 3), the homogenization speed is 50-1000 r.

7. The use of the synthetic raw material composition containing octuple hyaluronic acid for improving sensitive skin in a skin care product according to claim 4, characterized in that: The skin care product is an emulsion of a skin care product composition containing 5% octuple hyaluronic acid, and the preparation process is as follows: Phase A: sucrose poly-cottonseed oil acid ester 4 parts, tocopherol acetate 1.5 parts, thyme essential oil 0.5 parts; Phase B: glycerol 10 parts; Phase C: butanediol 5 parts, oat beta-glucan 0.05 parts, betaine 2 parts, baicalin 0.2 parts, sophoramine 0.1 parts, salicin 0.3 parts, p-hydroxyacetophenone 0.4 parts, 1,2-hexanediol 0.4 parts, the octuple hyaluronic acid-containing synthetic raw material composition of claim 1 5 parts, deionized water in residual amount; The components of phase A are added together into a reaction kettle, stirred at 30 r / m until completely dissolved, then the components of phase B are added, heated to 40-45°C and kept for about 20 minutes, stirred at 30 r / m until completely dissolved, then the components of phase C are added, and stirred (30 r / m) until completely dissolved, and then cooled to room temperature to obtain an octuple hyaluronic acid-containing emulsion.

8. The use of the octuple hyaluronic acid-containing synthetic raw material composition for improving sensitive skin according to claim 4 in skin care products, characterized in that, The skin care product is an essence containing 5% octuple hyaluronic acid, and the preparation process is as follows: Component A: PEG-40 hydrogenated castor oil 1 part, methyl polytrimethylsiloxane 5 parts, di-octyl carbonate 4 parts; Component B: glycerol 3 parts, 82.1% water; Component C: the octuple hyaluronic acid-containing synthetic raw material composition of claim 1 5 parts, multi-skin containing palmitoyl tri-skin-1 and palmitoyl tetra-skin-7 1 part, glycosphingolipid 1 part; (1) Stir the components of phase A at 80 rpm to 40-45°C, keep for 10 min, and reserve as oil phase raw material; (2) Put the components of phase B in a container, stir at 60 rpm to 40-45°C, keep for 20 min, and reserve as water phase raw material; (3) Add the water phase raw material into the emulsification homogenization device, start stirring, slowly suck the oil phase raw material into the emulsification device at 40-45°C, and obtain a mixed solution; start homogenization for 30 s at 5000 rpm; (4) After homogenization, keep the stirring speed unchanged, cool to 40°C, add component C, stir for 15 min, and discharge to obtain an octuple hyaluronic acid-containing essence.

9. The use of the octuple hyaluronic acid-containing synthetic raw material composition for improving sensitive skin according to claim 4 in skin care products, characterized in that: The addition amount of the synthetic raw material composition in the skin care product is 5%; the skin care product is a product in the form of water, cream, emulsion, and facial mask; The conventional adjuvants are selected from one or more of the mixtures of emulsifiers, surfactants, emollients, solubilizers, thickening agents, skin conditioning agents, humectants, pH regulators, preservatives, chelating agents, fragrances, and deionized water.

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