A KASP molecular marker for detecting the rice blast resistance gene Pi65 and its application

By developing KASP molecular markers and primer combinations based on SNP sites, the problem that InDel-1 molecular markers cannot directly identify the rice blast resistance gene Pi65 was solved, achieving efficient and low-cost genotyping and breeding improvement.

CN120924701BActive Publication Date: 2026-05-26HUAZHI RICE BIO TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHI RICE BIO TECH CO LTD
Filing Date
2025-07-28
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

In existing technologies, the InDel-1 molecular marker cannot directly obtain the genotype of the rice blast resistance gene Pi65, resulting in low detection throughput and high cost, making it difficult to efficiently screen for broad-spectrum and durable rice blast resistant varieties.

Method used

Develop KASP molecular markers based on SNP sites, including primer combinations and kits, for detecting the rice blast resistance gene Pi65. Use KASP technology for polymorphism detection and combine with gene chips to achieve high-throughput, low-cost genotyping.

Benefits of technology

It enables rapid and accurate identification of the rice blast resistance gene Pi65, increases detection throughput, reduces costs, minimizes laboratory human error, and is applicable to a wide range of breeding improvements.

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Abstract

This invention discloses a KASP molecular marker for detecting the rice blast resistance gene Pi65 and its application. The KASP molecular marker includes SNP1 and / or SNP2. SNP1 is located at 28,376,936 bp on chromosome 11 of the Nipponbare MSUv7 reference genome, with polymorphism of T or C. SNP2 is located at 28,377,512 bp on chromosome 11 of the Nipponbare MSUv7 reference genome, with polymorphism of A or C. The KASP molecular marker provided by this invention has the characteristics of high genotyping quality, single copy, and high polymorphism. It can rapidly, efficiently, and cost-effectively identify the genotype of the rice blast resistance gene Pi65, and can be used for marker-assisted breeding for rice blast resistance improvement, with wide applicability.
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