Zebra fish juvenile fish fixing device and fixing method thereof

Abstract

The invention relates to the technical field of biological experiments, in particular to a zebra fish juvenile fish fixing device and a fixing method thereof.The zebra fish juvenile fish fixing device comprises an observation plate, a fixing device and a fixing device, the placing seat is arranged in the groove, the bottom of the placing seat abuts against the bottom wall in the groove, a mounting groove is formed in the top of the placing seat, and the interior of the mounting groove is used for placing juvenile zebrafish. According to the zebra fish juvenile fish fixing device, through collaborative design of the magnetic type fixing base, clamping of the adjustable threaded rod and assisting of the controllable adhesive, non-damage rapid fixing of zebra fish juvenile fish is achieved, operation convenience, physiological protectiveness and experimental adaptability are achieved, and the stability and reliability of juvenile fish fixing are remarkably improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological experiments, in particular to a zebrafish larva fixing device and a fixing method thereof. BACKGROUND

[0002] In the fields of biomedical research and developmental biology, zebrafish larvae are widely used as an important model organism in various aspects such as gene expression, disease model construction, drug screening, and developmental process research. Due to their small size, strong activity, and sensitivity to the external environment, it is a big challenge for researchers to effectively fix the larvae and keep them in a specific posture for precise observation and operation while minimizing harm and interference to the larvae. Traditional methods of fixing zebrafish larvae often have many shortcomings. For example, some methods use physical restraint, such as using fine wires or fibers to tie and fix the larvae. Although this method is simple and direct, it can cause mechanical damage to the larvae's body, affect their normal physiological functions, and even cause death, thereby affecting the accuracy and reliability of experimental results. In addition, physical restraint can also limit the natural activity of the larvae, making them in a non-physiological state, which is not conducive to studying the normal behavior and physiological processes of the larvae. Other methods use chemical adhesives to fix the larvae, but common adhesives may have problems such as high toxicity and difficult to control the setting time. High toxicity adhesives can harm the larvae and affect their survival rate and subsequent growth and development; and too long or too short setting time is not conducive to experimental operation, too long can cause the larvae to move position before the adhesive sets, affecting the fixing effect, and too short can make researchers unable to adjust the posture of the larvae before it is fixed, which cannot meet the ideal observation and experimental requirements. SUMMARY

[0003] In view of the above problems, the present application provides a zebrafish larva fixing device and a fixing method thereof to solve at least one of the above problems.

[0004] In the first aspect, the present application provides a zebrafish larva fixing device, comprising: an observation plate, the top of which is provided with a plurality of grooves;

[0005] a placing seat, which is arranged inside the groove, the bottom of the placing seat abuts against the bottom wall inside the groove, and the top of the placing seat is provided with a mounting groove, the inside of which is used to place zebrafish larvae.

[0006] In some embodiments, the transverse cross-section of the observation plate is a rectangular structure, and a plurality of grooves are arranged in an array on the top of the observation plate.

[0007] In some embodiments, a base is provided at the bottom of the groove, the base has a cross-shaped cross section, and a placement groove is provided at the center point of the base.

[0008] In some embodiments, a magnet is disposed inside the placement groove, the outer diameter of the magnet matches the inner diameter of the placement groove, the bottom of the magnet is fixedly connected to the bottom wall inside the placement groove, and the depth of the placement groove is greater than the height of the magnet.

[0009] In some embodiments, the bottom of the placement base is provided with a magnetic suction member that matches the placement slot. The magnetic suction member is fixedly connected to the bottom of the placement base and is used to connect with the magnet.

[0010] In some embodiments, the distance between the magnet and the top of the placement slot is matched with the height of the magnetic attractor.

[0011] In some embodiments, two fixing components are symmetrically arranged inside the mounting slot, the fixing components including:

[0012] A first baffle is disposed at the top of the mounting groove. The first baffle is arranged horizontally, and the side wall of the first baffle is fixedly connected to the inner wall of the mounting groove.

[0013] A second baffle is disposed inside the mounting groove. The second baffle is vertically disposed. The top of the second baffle is fixedly connected to the first baffle, and the bottom of the second baffle is fixedly connected to the bottom wall of the mounting groove. A cavity is formed between the first baffle, the second baffle, and the inner wall of the mounting groove.

[0014] A telescopic assembly is disposed inside the chamber, and the telescopic end of the telescopic assembly extends through the second baffle into the interior of the mounting groove.

[0015] In some embodiments, the telescopic component includes:

[0016] A first threaded rod is vertically disposed inside the chamber, the top of the first threaded rod extends through the first baffle to the top of the first baffle, and the first threaded rod is rotatably connected to the first baffle;

[0017] The rotating seat has its bottom fixedly connected to the bottom wall inside the mounting groove, and the bottom of the first threaded rod is rotatably connected to the top of the rotating seat.

[0018] In some embodiments, the telescopic component further includes:

[0019] The second threaded rod is laterally disposed inside the cavity. A baffle is fixedly connected to the left end of the second threaded rod, and the right end of the second threaded rod extends through the second baffle to the outside of the cavity. The second threaded rod is engaged with the first threaded rod and rotatably connected to the second baffle.

[0020] A fixing plate is vertically disposed inside the mounting groove, and one end of the fixing plate is fixedly connected to the right end of the second threaded rod.

[0021] Secondly, the present invention provides a method for fixing juvenile zebrafish, comprising the following steps:

[0022] S1. Place the fish anesthetic and adhesive into the installation tank, and then place the zebrafish fry into the installation tank.

[0023] S2. Rotate the two first threaded rods to adjust the distance between the two fixing plates to fix the zebrafish fry;

[0024] S3. Observe the zebrafish juveniles after the adhesive has solidified.

[0025] Compared with existing technologies, the advantages of this invention are as follows: The magnetic connection between the placement seat and the base avoids the potential strangulation injuries that can occur when binding juvenile fish with thin threads. When fixing the juvenile fish, the distance between the two fixing plates is adjusted using two first threaded rods, resulting in a relatively gentle fixation method that avoids excessive compression or cutting damage to the juvenile fish's body. The overall design of the device takes into account the physiological characteristics of juvenile fish, minimizing direct contact and force during fixation, thus helping to maintain the integrity and normal physiological state of the juvenile fish. Although an adhesive is used in the fixation method, by reasonably controlling its dosage and selecting a suitable adhesive, combined with the use of an anesthetic, the harm to the juvenile fish can be reduced while ensuring the fixation effect. Furthermore, the device is designed so that the juvenile fish's posture can be adjusted by adjusting the fixing plates before the adhesive solidifies, avoiding situations where the juvenile fish are fixed in an unfavorable posture or suffer excessive chemical damage due to the difficulty in controlling the adhesive's solidification time. Adjusting the distance between the fixing plates by rotating the two first threaded rods allows for convenient fixation and posture adjustment of the juvenile fish. Researchers can precisely control the position and posture of juvenile fish according to the needs of experimental observation, so as to put them in the best observation state, which helps to improve the accuracy and reliability of the experiment.

[0026] The above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit this disclosure.

[0027] Other features and aspects of this disclosure will become clearer from the following detailed description of exemplary embodiments with reference to the accompanying drawings. Attached Figure Description

[0028] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0029] Figure 1 This is a top view of the zebrafish juvenile restraint device provided in an embodiment of the present invention;

[0030] Figure 2 This is a partial enlarged view of the zebrafish juvenile restraint device provided in an embodiment of the present invention;

[0031] Figure 3 This is a partial enlarged view of the zebrafish juvenile restraint device provided in an embodiment of the present invention;

[0032] Figure 4 This is a cross-sectional view of the zebrafish juvenile restraint device provided in an embodiment of the present invention.

[0033] The components are: 1. Observation plate; 2. Groove; 3. Placement seat; 4. Mounting slot; 5. Base; 6. Placement slot; 7. Magnet; 8. Magnetic suction component; 9. First baffle; 10. Second baffle; 11. Chamber; 12. First threaded rod; 13. Rotating seat; 14. Second threaded rod; 15. Baffle; 16. Fixing plate. Detailed Implementation

[0034] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0035] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0036] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0037] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0038] See Figures 1-4 As shown in the first embodiment, a zebrafish juvenile restraint device according to an embodiment of this application includes:

[0039] The observation plate 1 has multiple grooves 2 on its top;

[0040] Placement seat 3 is disposed inside the groove 2. The bottom of placement seat 3 abuts against the bottom wall inside the groove 2. The top of placement seat 3 is provided with mounting groove 4. The interior of mounting groove 4 is used to place zebrafish fry.

[0041] In some specific embodiments, the transverse cross-section of the observation plate 1 is a rectangular structure, and a plurality of the grooves 2 are arrayed on the top of the observation plate 1.

[0042] In some specific embodiments, a base 5 is provided at the bottom of the groove 2, the cross section of the base 5 is a cross-shaped structure, and a placement groove 6 is provided at the center point of the base 5.

[0043] In some specific embodiments, a magnet 7 is provided inside the placement groove 6, the outer diameter of the magnet 7 matches the inner diameter of the placement groove 6, the bottom of the magnet 7 is fixedly connected to the bottom wall inside the placement groove 6, and the depth of the placement groove 6 is greater than the height of the magnet 7.

[0044] In some specific embodiments, the bottom of the placement base 3 is provided with a magnetic suction member 8 that matches the placement groove 6. The magnetic suction member 8 is fixedly connected to the bottom of the placement base 3 and is used to connect with the magnet 7.

[0045] In some specific embodiments, the distance between the magnet 7 and the top of the placement slot 6 is matched with the height of the magnetic attractor 8.

[0046] It should be understood that the bottom of the groove 2 of the observation plate 1 is provided with a cross-shaped base 5, and the center of the base is provided with a placement groove 6, in which a magnet 7 is fixed. The bottom of the placement seat 3 is provided with a magnetic suction piece 8 that matches the placement groove 6. Through the magnetic attraction between the magnet 7 and the magnetic suction piece 8, the placement seat 3 is fixed to the base 5. The distance between the magnet 7 and the top of the placement groove 6 is designed to match the height of the magnetic suction piece 8, ensuring that the magnetic suction piece 8 can completely fit the magnet 7 and form a stable connection. The magnetic connection does not require complicated operation. Simply align the placement seat 3 with the magnetic suction piece 8 and gently place it on the base 5, and it will automatically attract and fix itself. After the experiment, the magnetic suction piece 8 and the magnet 7 can be separated by external force (such as gentle pulling) to achieve quick disassembly of the placement seat 3, facilitating the transfer of juvenile fish without damage.

[0047] The base 5 has a cross-shaped cross section, which enhances the connection stability between the base and the observation plate 1 and prevents the base from shifting due to magnetic force or external vibration. The cross-shaped structure also provides multi-directional support for the placement seat 3, preventing it from tilting or rotating under magnetic force. By replacing the placement seat 3 with different sizes (such as adjusting the size of the mounting slot 4), it can be adapted to zebrafish juveniles at different developmental stages or in different body sizes.

[0048] The magnetic design enables "instant placement and fixation," eliminating the need for complex procedures (such as tying lines or adjusting adhesive), thus saving experimental time. The quick-release function facilitates the non-destructive transfer of juvenile fish after the experiment, preventing injury caused by adhesion or excessive tightness of the fixation device. By replacing the placement base 3 with different sizes, it can easily adapt to juvenile fish at different developmental stages or with varying body sizes, improving the device's versatility and applicability. The standardized design of the magnetic component 8 and the magnet 7 ensures compatibility with different placement bases 3, facilitating modular replacement and maintenance.

[0049] In some specific embodiments, two fixing components are symmetrically arranged inside the mounting slot 4, and the fixing components include:

[0050] A first baffle 9 is disposed at the top of the mounting groove 4. The first baffle 9 is arranged horizontally, and the side wall of the first baffle 9 is fixedly connected to the inner wall of the mounting groove 4.

[0051] The second baffle 10 is disposed inside the mounting groove 4. The second baffle 10 is vertically disposed. The top of the second baffle 10 is fixedly connected to the first baffle 9, and the bottom of the second baffle 10 is fixedly connected to the bottom wall of the mounting groove 4. A cavity 11 is formed between the first baffle 9, the second baffle 10, and the inner wall of the mounting groove 4.

[0052] A telescopic assembly is disposed inside the chamber 11, and the telescopic end of the telescopic assembly extends through the second baffle 10 into the interior of the mounting groove 4.

[0053] In some specific embodiments, the telescopic component includes:

[0054] The first threaded rod 12 is vertically disposed inside the chamber 11. The top of the first threaded rod 12 extends through the first baffle 9 to the top of the first baffle 9. The first threaded rod 12 is rotatably connected to the first baffle 9.

[0055] The bottom of the rotating seat 13 is fixedly connected to the bottom wall inside the mounting groove 4, and the bottom of the first threaded rod 12 is rotatably connected to the top of the rotating seat 13.

[0056] In some specific embodiments, the telescopic component further includes:

[0057] The second threaded rod 14 is laterally disposed inside the chamber 11. A baffle 15 is fixedly connected to the left end of the second threaded rod 14. The right end of the second threaded rod 14 extends through the second baffle 10 to the outside of the chamber 11. The second threaded rod 14 is engaged with the first threaded rod 12. The second threaded rod 14 is rotatably connected to the second baffle 10.

[0058] A fixing plate 16 is vertically disposed inside the mounting groove 4, and one end of the fixing plate 16 is fixedly connected to the right end of the second threaded rod 14.

[0059] It should be understood that the first threaded rod 12 is vertically positioned, with its top passing through the first baffle 9 and rotatable, and its bottom connected to the bottom wall of the mounting groove 4 via a rotating seat 13. Rotating the first threaded rod 12 causes it to rotate. The second threaded rod 14 is horizontally positioned, with its left end fixed to the baffle 15 and its right end passing through the second baffle 10 and extending to the outside of the chamber, engaging with the first threaded rod 12. When the first threaded rod 12 rotates, the second threaded rod 14 moves horizontally due to the threaded engagement. The fixing plate 16 is vertically positioned within the mounting groove 4, with one end fixedly connected to the right end of the second threaded rod 14. When the second threaded rod 14 moves horizontally, it causes the fixing plate 16 to move horizontally, adjusting the distance between the two fixing plates 16. After placing the zebrafish fry into the mounting slot 4, rotate the first threaded rod 12 to drive the second threaded rod 14 to move laterally, causing the two fixing plates 16 to move closer together and gradually clamp the fry. The vertical design of the fixing plates 16 can limit the vertical displacement of the fry in the mounting slot 4. Combined with the stable support of the magnetic base, the fry can be fixed in all directions.

[0060] By rotating the first threaded rod 12 to drive the second threaded rod 14, the movement distance of the fixing plate 16 can be precisely controlled, achieving gentle clamping of the juvenile fish. The threaded structure has self-locking properties, making it difficult to loosen after fixing, ensuring that the juvenile fish maintain a stable posture during experiments. The spacing of the fixing plates 16 is adjustable to accommodate zebrafish juveniles at different developmental stages or in different body sizes. For example, smaller juveniles can be fixed with a smaller spacing, while larger juveniles can be fixed with a larger spacing to avoid being too tight or too loose. The vertical fixing plates 16 can restrict the vertical movement of the juvenile fish, and together with the lateral fixation of the magnetic base, form a three-dimensional constraint, making it more versatile. The fixing plates 16 adopt a smooth vertical clamping method, avoiding the marks or localized pressure that may be caused to the juvenile fish's body by traditional binding or clamping. The slow clamping process driven by the threads can prevent injury to the juvenile fish caused by excessive force, protecting their physiological state.

[0061] The moving of the fixing plate 16 is achieved by rotating the first threaded rod 12, making the operation simple and quick without the need for complex tools or auxiliary equipment. The symmetrically designed fixing components allow simultaneous adjustment of both fixing plates 16, ensuring the juvenile fish are centered and fixed, thus improving operational efficiency. The fixing components (such as the threaded rod, baffle, and fixing plate) are all designed as independent parts, facilitating disassembly, replacement, or maintenance, reducing maintenance costs. The closed structure of the chamber 11 prevents experimental liquids (such as anesthetics and adhesives) from entering the threaded mechanism, extending the lifespan of the device. The vertical clamping of the fixing plate 16 combined with the lateral fixation of the magnetic base forms a double constraint, effectively preventing the juvenile fish from shifting due to struggling or external vibrations during the experiment.

[0062] A second embodiment of a zebrafish juvenile fixation method according to an embodiment of this application includes the following steps:

[0063] S1. Place the fish anesthetic and adhesive into the installation tank, and then place the zebrafish fry into the installation tank.

[0064] S2. Rotate the two first threaded rods to adjust the distance between the two fixing plates to fix the zebrafish fry;

[0065] S3. Observe the zebrafish juveniles after the adhesive has solidified.

[0066] It should be understood that anesthetics can reduce the struggling and stress response of juvenile fish during the fixation process, avoiding damage caused by mechanical pressure or friction. Adhesives are used to assist in fixation, reducing the direct binding force on the juvenile fish's body and lowering the risk of mechanical damage. Fixation is achieved through adjusting the spacing of the fixation plates and the solidification of the adhesive, avoiding the marks or local ischemia that may be caused to the juvenile fish's body surface by traditional binding or clamping.

[0067] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A device for securing juvenile zebrafish, characterized in that, include: The observation plate has multiple grooves on its top. A placement seat is provided inside the groove, with the bottom of the placement seat abutting against the bottom wall inside the groove, and the top of the placement seat is provided with an installation groove, the interior of which is used to place zebrafish fry.

2. The zebrafish juvenile restraint device according to claim 1, characterized in that, The observation plate has a rectangular cross-section, and multiple groove arrays are arranged on the top of the observation plate.

3. The zebrafish juvenile fixation device according to claim 2, characterized in that, The bottom of the groove is provided with a base, the cross section of the base is a cross-shaped structure, and the center point of the base is provided with a placement groove.

4. The zebrafish juvenile fixation device according to claim 3, characterized in that, A magnet is installed inside the placement groove. The outer diameter of the magnet matches the inner diameter of the placement groove. The bottom of the magnet is fixedly connected to the bottom wall inside the placement groove. The depth of the placement groove is greater than the height of the magnet.

5. A zebrafish juvenile restraint device according to claim 4, characterized in that, The bottom of the placement base is provided with a magnetic suction member that matches the placement slot. The magnetic suction member is fixedly connected to the bottom of the placement base and is used to connect with the magnet.

6. A zebrafish juvenile restraint device according to claim 5, characterized in that, The distance between the magnet and the top of the placement slot is matched with the height of the magnetic attractor.

7. A zebrafish juvenile restraint device according to claim 6, characterized in that, Two fixing components are symmetrically arranged inside the mounting slot. The fixing components include: A first baffle is disposed at the top of the mounting groove. The first baffle is arranged horizontally, and the side wall of the first baffle is fixedly connected to the inner wall of the mounting groove. A second baffle is disposed inside the mounting groove. The second baffle is vertically disposed. The top of the second baffle is fixedly connected to the first baffle, and the bottom of the second baffle is fixedly connected to the bottom wall of the mounting groove. A cavity is formed between the first baffle, the second baffle, and the inner wall of the mounting groove. A telescopic assembly is disposed inside the chamber, and the telescopic end of the telescopic assembly extends through the second baffle into the interior of the mounting groove.

8. A zebrafish juvenile restraint device according to claim 7, characterized in that, The telescopic component includes: A first threaded rod is vertically disposed inside the chamber, the top of the first threaded rod extends through the first baffle to the top of the first baffle, and the first threaded rod is rotatably connected to the first baffle; The rotating seat has its bottom fixedly connected to the bottom wall inside the mounting groove, and the bottom of the first threaded rod is rotatably connected to the top of the rotating seat.

9. A zebrafish juvenile restraint device according to claim 8, characterized in that, The telescopic component also includes: The second threaded rod is laterally disposed inside the cavity. A baffle is fixedly connected to the left end of the second threaded rod, and the right end of the second threaded rod extends through the second baffle to the outside of the cavity. The second threaded rod is engaged with the first threaded rod and rotatably connected to the second baffle. A fixing plate is vertically disposed inside the mounting groove, and one end of the fixing plate is fixedly connected to the right end of the second threaded rod.

10. A method for fixing juvenile zebrafish, characterized in that, The zebrafish juvenile restraint device according to any one of claims 1 to 9 comprises the following steps: S1. Place the fish anesthetic and adhesive into the installation tank, and then place the zebrafish fry into the installation tank. S2. Rotate the two first threaded rods to adjust the distance between the two fixing plates to fix the zebrafish fry; S3. Observe the zebrafish juveniles after the adhesive has solidified.

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