Detection method for improving detection of terpene substances in yeast for making hard liquor

By combining anhydrous ethanol water bath rotary evaporation and nitrogen purging with multi-stage cold trap concentration technology, the problems of matrix complexity and trace amounts in the detection of terpenes in Daqu (a type of Chinese liquor) have been solved, achieving efficient and accurate detection of terpenes.

CN120971592APending Publication Date: 2025-11-18JIANGNAN UNIV +2
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Patent Information

Application Number
CN202510991224.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-18
Publication Date
2025-11-18

AI Technical Summary

Technical Problem

Existing technologies for detecting terpenoids in Daqu (a type of Chinese liquor) suffer from problems such as complex matrices, trace amounts, difficulty in distinguishing isomers, and pretreatment losses, resulting in inefficient and inaccurate detection methods.

Method used

The Daqu samples were pretreated and volatile components were enriched by using anhydrous ethanol rotary evaporation in a water bath combined with nitrogen purging and multi-stage cold trap concentration techniques, and then analyzed by gas chromatography-mass spectrometry.

Benefits of technology

It significantly improves the detection rate and accuracy of terpenoids, enabling the detection of 12 terpenoids in one go, simplifying the sample processing process and reducing the influence of interfering substances.

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Abstract

The invention provides a detection method for improving detection of terpene substances in yeast for making hard liquor, and relates to the field of flavor analysis of fermented food. The method comprises the following steps: 1) performing crushing pretreatment on a target yeast block to obtain yeast powder; 2) taking absolute ethyl alcohol as a solvent, performing water bath rotary evaporation on the yeast powder at 40-60 DEG C, filtering after rotary evaporation, retaining solid-phase yeast powder, and performing vacuum drying to obtain a dried yeast powder sample; (3) collecting a gas-phase sample with stable components volatilized from the yeast powder sample under the conditions of water bath heating and nitrogen purging; (4) pre-concentrating the gas-phase sample by adopting a multi-stage cold trap principle, wherein the enrichment multiple of the gas-phase sample is not less than 1000 in the pre-concentration; 5) collecting the pre-concentrated gas phase sample, inputting the pre-concentrated gas phase sample into a gas chromatograph-mass spectrometer for gas chromatography-mass spectrometry, and determining the terpene substances in the yeast for making hard liquor through standard gas matching. Compared with the prior art, the method has the advantages that 12 terpene substances can be simply and efficiently detected at a time, and the detection rate of the terpene substances is remarkably increased.
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