A rapid detection pen for mycoplasma pneumoniae antibody

Abstract

The application relates to the technical field of detection of mycoplasma pneumoniae, in particular to a rapid detection pen for mycoplasma pneumoniae antibodies, which comprises a box body, one end of the box body is fixedly connected with a drawing cylinder, the other end of the box body is provided with a negative pressure device, a connecting hole is formed through the box body, the two ends of the connecting hole are respectively communicated with the negative pressure device and the drawing cylinder, an installation groove is formed in the box body, a through hole is formed through the installation groove and the connecting hole, a filter layer is fixedly installed in the installation groove, and a detection test paper is fixedly connected to the upper surface of the filter layer. In use, the nasal cavity under different conditions can be sampled by switching modes, and the nasal mucus is drawn into the connecting hole and flows into the installation groove by pressing the negative pressure device, the filter layer removes impurities, optimizes sample concentration, protects target substances and reduces interference, and then flows to the detection test paper for detection, so that the equipment is simple and convenient to use and is convenient to use at home.

Description

Technical Field

[0001] This invention relates to the field of Mycoplasma pneumoniae detection technology, specifically to a rapid detection pen for Mycoplasma pneumoniae antibodies. Background Technology

[0002] Mycoplasma pneumoniae (MP) is a unique microorganism and one of the most common pathogens causing acute respiratory infections in children, as well as a significant cause of acute respiratory infections in humans. It occurs in epidemics approximately every 4-5 years, primarily in the spring. Each year, about 10% of adult pneumonia and 20%-25% of childhood pneumonia are caused by Mycoplasma pneumoniae. Clinical symptoms are diverse. Transmitted through the respiratory tract, fever and cough are prominent symptoms. In severe cases, it can cause multi-organ damage, with reports of complications such as myocardial and liver damage. In addition, Mycoplasma pneumoniae can cause pharyngitis, rhinitis, otitis media, tracheitis, and bronchitis, and may develop into interstitial pneumonia during the infection period. It is often difficult to differentiate from viral, bacterial, and tuberculosis infections based solely on clinical manifestations. Because Mycoplasma pneumoniae is resistant to common drugs, early detection, early treatment, and minimizing complications are urgent problems to be solved.

[0003] Currently, most patients infected with Mycoplasma pneumoniae initially mistake it for a cold. Some patients with mild cold symptoms do not go to the hospital for testing and treatment, which allows the disease to progress rapidly and affects later treatment. Therefore, there is a need for a device that can detect Mycoplasma pneumoniae antibodies at home, and it is necessary to propose a rapid Mycoplasma pneumoniae antibody test pen. Summary of the Invention

[0004] To address the problems in the prior art, this invention provides a rapid detection pen for Mycoplasma pneumoniae antibodies.

[0005] The technical solution adopted by this invention to solve its technical problem is as follows: a rapid detection pen for mycoplasma pneumoniae antibodies, comprising a box body, an extraction tube fixedly connected to one end of the box body, a negative pressure device provided at the other end of the box body, a connecting hole through the box body, the two ends of the connecting hole being connected to the negative pressure device and the extraction tube respectively, an installation groove on the box body, a through hole through the installation groove and the connecting hole, a filter layer fixedly installed in the installation groove, a test strip fixedly connected to the upper surface of the filter layer, an observation window fixedly installed at the opening of the installation groove, a storage tube fixedly installed at one end of the box body located inside the extraction tube, a rotating hole on the box body, a threaded sleeve rotatably connected to the rotating hole via a bearing, a drive wheel fixedly sleeved on the outside of the threaded sleeve, a threaded block threadedly connected inside the threaded sleeve, a rectangular rod fixedly connected to the threaded block, one end of the rectangular rod extending into the storage tube and fixedly connected to a sampling swab, and a rectangular sleeve fixedly installed inside the storage tube.

[0006] Specifically, the negative pressure device includes a fixed groove, which is formed on the box body. Elastic compression plates are fixedly connected to the upper and lower walls of the fixed groove, and an airbag is fixedly installed between the fixed groove and the elastic compression plates.

[0007] Specifically, a grid plate is fixedly installed inside the connecting hole, and the grid plate is located on one side of the through hole.

[0008] Specifically, the inner hole of the rectangular sleeve is rectangular, and the rectangular rod is slidably connected in the inner hole of the rectangular sleeve.

[0009] Specifically, the threaded sleeve is open at one end, and the open end of the threaded sleeve extends into the receiving tube and abuts against the rectangular sleeve.

[0010] Specifically, the sampling swab is fitted inside the storage tube and abuts against the inner wall of the storage tube.

[0011] Specifically, the storage tube is fitted inside the extraction tube, and the opening of the connecting hole is opened in the gap between the storage tube and the extraction tube.

[0012] The beneficial effects of the present invention: The rapid detection pen for Mycoplasma pneumoniae antibodies described in this invention, when in use, creates negative pressure by pressing the negative pressure device, then inserts the extraction tube into the nasal cavity to collect nasal mucus, or pushes out the sampling swab and collects a sample in the nasal cavity, and then retracts the sampling swab. When the sampling swab comes into contact with the opening of the storage tube, it scrapes off the nasal mucus. The negative pressure device can be released and the extraction tube can be used to collect nasal mucus. The nasal mucus is drawn into the connection hole and flows into the installation groove, where it undergoes impurity removal, sample concentration optimization, protection of target substances, and reduction of interference, before flowing to the test strip for detection. This makes the device simple, convenient, and easy to use at home. Attached Figure Description

[0013] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0014] Figure 1 This is a schematic diagram of the external structure of a rapid detection pen for Mycoplasma pneumoniae antibodies in the shallow sampling state, provided by the present invention.

[0015] Figure 2 This is a schematic diagram of the external structure of a rapid detection pen for Mycoplasma pneumoniae antibodies in a deep sampling state, provided by the present invention.

[0016] Figure 3 A top view of the internal structure of a rapid detection pen for Mycoplasma pneumoniae antibodies in the shallow sampling state, provided by the present invention.

[0017] Figure 4 A top view of the internal structure of a rapid detection pen for Mycoplasma pneumoniae antibodies in deep sampling state, provided by the present invention.

[0018] Figure 5 A side view of the internal structure of a rapid detection pen for Mycoplasma pneumoniae antibodies in the shallow sampling state, provided by the present invention.

[0019] Figure 6 A schematic diagram of the internal structure of a sampling swab for a rapid detection pen for Mycoplasma pneumoniae antibodies provided by the present invention;

[0020] Figure 7 This is a schematic diagram of the external structure of the sampling swab for a rapid detection pen for Mycoplasma pneumoniae antibodies provided by the present invention.

[0021] In the diagram: 1. Box body; 2. Extraction tube; 3. Connection hole; 4. Mounting slot; 5. Through hole; 6. Filter layer; 7. Test paper; 8. Observation window; 9. Storage tube; 10. Rotation hole; 11. Threaded sleeve; 12. Drive wheel; 13. Threaded block; 14. Rectangular rod; 15. Sampling swab; 16. Rectangular sleeve; 17. Fixing slot; 18. Elastic compression plate; 19. Airbag; 20. Grid plate. Detailed Implementation

[0022] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0023] like Figures 1-7 As shown, the present invention provides the following technical solution:

[0024] Example 1: A rapid test pen for Mycoplasma pneumoniae antibodies includes a housing 1, with an extraction tube 2 fixedly connected to one end of the housing 1 and a negative pressure device at the other end. A connection hole 3 is formed through the housing 1, with both ends of the connection hole 3 communicating with the negative pressure device and the extraction tube 2, respectively. An installation groove 4 is formed on the housing 1, with a through hole 5 between the installation groove 4 and the connection hole 3. A filter layer 6 is fixedly installed within the installation groove 4. The filter layer 6 includes sterile gauze soaked in phosphate buffer and a sponge layer soaked in sample diluent, and an isolation layer is provided between it and a test strip 7. A test strip 7, a colloidal gold test strip, is fixedly connected to the upper surface of the filter layer 6. The test strip 7 has a detection line (T line) on the side closer to the negative pressure device and a quality control line (C line) on the side closer to the extraction tube 2. The groove opening of the installation groove 4... An observation window 8 is fixedly installed. A storage tube 9 is fixedly installed at one end of the box body 1 inside the extraction tube 2. A rotating hole 10 is opened on the box body 1. A threaded sleeve 11 is rotatably connected to the rotating hole 10 through a bearing. A drive wheel 12 is fixedly sleeved on the outside of the threaded sleeve 11. A threaded block 13 is threadedly connected inside the threaded sleeve 11. A rectangular rod 14 is fixedly connected to the threaded block 13. One end of the rectangular rod 14 extends into the storage tube 9 and is fixedly connected to a sampling swab 15. A rectangular sleeve 16 is fixedly installed inside the storage tube 9. The negative pressure device includes a fixed groove 17. The fixed groove 17 is opened on the box body 1. An elastic extrusion plate 18 is fixedly connected to the upper and lower groove walls of the fixed groove 17. An airbag 19 is fixedly installed between the fixed groove 17 and the elastic extrusion plate 18. A grid plate 20 is fixedly installed in the connection hole 3. The grid plate 20 is set on one side of the through hole 5.

[0025] When using it, the following steps are included:

[0026] First, check the amount of nasal mucus in your nasal cavity, then press the elastic squeezing plate 18. The elastic squeezing plate 18 will collapse into the fixing groove 17 and squeeze the airbag 19, while the air in the airbag 19 will be discharged through the connecting hole 3.

[0027] The second step is to insert the extraction tube 2 directly into the nostril when there is a lot of nasal mucus. Then you can release the elastic squeezing plate 18. The elastic squeezing plate 18 will reset itself under its own elasticity. When the elastic squeezing plate 18 resets, it will pull the upper and lower ends of the airbag, causing the airbag 19 to open and form negative pressure. The nasal mucus in the extraction hole 2 will be drawn into the connection hole 3 through the connection hole 3.

[0028] Thirdly, or if there is little nasal discharge, rotate the drive wheel 12. The drive wheel 12 drives the threaded sleeve 11 to rotate, while the rectangular sleeve 16 limits the rectangular rod 14, preventing the threaded block 13, the rectangular rod 14, and the sampling swab 15 from rotating. As the threaded sleeve 11 rotates, it drives the threaded block 13, the rectangular rod 14, and the sampling swab 15 to move outward, extending them beyond the extraction tube 2. Gently insert the sampling swab 15 into the nasal cavity 1-2 cm (0.5-1 cm for children), rotate clockwise 2-3 times, hold for 5 seconds, and then suction. After collecting the secretions, pull out the sampling swab 15 and then rotate the drive wheel 12 in the opposite direction to pull the sampling swab 15 back into the storage tube 9. When the sampling swab 15 enters the storage tube 9, it will scrape against the edge of the opening of the storage tube 9, thereby scraping off the collected nasal mucus and letting it fall between the storage tube 9 and the extraction tube 2. The elastic squeezing plate 18 can be released, and the elastic squeezing plate 18 will reset itself under its own elasticity. When the elastic squeezing plate 18 resets, it will pull the upper and lower ends of the airbag, causing the airbag 10 to open and form a negative pressure, and then draw the nasal mucus in the extraction hole 2 into the connection hole 3 through the connection hole 3.

[0029] Fourth step: First, place the box 1 vertically for one minute, allowing the mucus in the connection hole 3 to flow downwards. Under the obstruction of the grid plate 20, most of the mucus will flow through the through hole 5 into the filter layer 6 in the mounting slot 4. When the mucus enters, it will first pass through sterile gauze soaked in phosphate buffer to filter out impurities and mucus, obtaining a clear liquid. Then, place the box 1 horizontally with the observation window 8 facing upwards for ten minutes. The clear liquid will flow to the bottom of the mounting slot 4 and enter the sponge layer soaked in the sample diluent. The diluent (containing Tris-HCl, with a pH value mostly maintained at 7.2-7.4 to maintain the antibody survival environment and prevent antibody denaturation; also containing bovine serum albumin to reduce non-specific adsorption; and sodium chloride to adjust the ionic strength of the sample and reduce the interference of mucus proteins, enzymes, and other components in mucus and saliva on the detection) can adjust the pH value and ionic strength of the clear liquid, inhibit interfering substances, and improve the accuracy and reliability of antibody detection.

[0030] Fifth step: After ten minutes, the clear liquid will mix with the diluent and precipitate the supernatant. Then, invert the box 1 and place the box 1 in a negative pressure downward tilt position for 30 seconds, so that the supernatant will flow to the test strip 7 first, and the test strip 7 will detect the antibodies in the supernatant.

[0031] Step 6: Observe the test strip 7 through the observation window 8, and the test result can be obtained through the following information about the test strip 7;

[0032] 1) Positive, both the test line (T line) and the control line (C line) show color, indicating the presence of Mycoplasma pneumoniae antibodies;

[0033] 2) Negative: Only the control line (C line) shows color, the test line (T line) shows no color.

[0034] 3) Invalid: The control line (C line) does not develop color and needs to be retested.

[0035] Example 2: The technical solutions in this example that differ from Example 1 include:

[0036] The rectangular sleeve 16 has a rectangular inner hole, and the rectangular rod 14 is slidably connected in the inner hole of the rectangular sleeve 16. The threaded sleeve 11 is open at one end, and the open end of the threaded sleeve 11 extends into the storage tube 9 and abuts against the rectangular sleeve 16. The sampling swab 15 is fitted inside the storage tube 9 and abuts against the inner wall of the storage tube 9. The storage tube 9 is fitted inside the extraction tube 2, and the opening of the connecting hole 3 is opened in the gap between the storage tube 9 and the extraction tube 2. The rectangular sleeve 16 passes through the inner hole. The rectangular hole in the part limits the rectangular rod 14, so that the threaded block 13, the rectangular rod 14 and the sampling swab 15 cannot rotate. Then, when the threaded sleeve 11 rotates, it will drive the threaded block 13, the rectangular rod 14 and the sampling swab 15 to move outward. When the sampling swab 15 is retrieved, it will abut against the opening of the collection tube 9, thereby scraping off the collected nasal mucus and letting it fall between the collection tube 9 and the extraction tube 2, which is convenient for collecting deeper nasal mucus.

[0037] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of protection claimed by the present invention. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A rapid detection pen for Mycoplasma pneumoniae antibodies, comprising a housing (1), characterized in that, One end of the box body (1) is fixedly connected to an extraction cylinder (2), and the other end of the box body (1) is provided with a negative pressure device. A connecting hole (3) is opened through the box body (1), and the two ends of the connecting hole (3) are respectively connected to the negative pressure device and the extraction cylinder (2). An installation groove (4) is opened on the box body (1), and a through hole (5) is opened through the installation groove (4) and the connecting hole (3). A filter layer (6) is fixedly installed in the installation groove (4), and a test paper (7) is fixedly connected to the upper surface of the filter layer (6). An observation window (8) is fixedly installed at the opening of the installation groove (4). The box body (1) is fixedly installed with a storage tube (9) at one end inside the extraction tube (2). The box body (1) has a rotating hole (10). A threaded sleeve (11) is rotatably connected to the rotating hole (10) through a bearing. A drive wheel (12) is fixedly sleeved on the outside of the threaded sleeve (11). A threaded block (13) is threadedly connected inside the threaded sleeve (11). A rectangular rod (14) is fixedly connected to the threaded block (13). One end of the rectangular rod (14) extends into the storage tube (9) and is fixedly connected to a sampling swab (15). A rectangular sleeve (16) is fixedly installed inside the storage tube (9). The negative pressure device includes a fixed groove (17), which is opened on the box body (1). An elastic compression plate (18) is fixedly connected to the upper and lower groove walls of the fixed groove (17). An airbag (19) is fixedly installed between the fixed groove (17) and the elastic compression plate (18). The threaded sleeve (11) is open at one end, and the open end of the threaded sleeve (11) extends into the receiving tube (9) and abuts against the rectangular sleeve (16). The sampling swab (15) is fitted inside the storage tube (9) and abuts against the inner wall of the storage tube (9).

2. The rapid detection pen for Mycoplasma pneumoniae antibodies according to claim 1, characterized in that: A grid plate (20) is fixedly installed inside the connecting hole (3), and the grid plate (20) is located on one side of the through hole (5).

3. The rapid detection pen for Mycoplasma pneumoniae antibodies according to claim 1, characterized in that: The inner hole of the rectangular sleeve (16) is rectangular, and the rectangular rod (14) is slidably connected in the inner hole of the rectangular sleeve (16).

4. The rapid detection pen for Mycoplasma pneumoniae antibodies according to claim 1, characterized in that: The storage tube (9) is fitted inside the extraction tube (2), and the opening of the connecting hole (3) is opened in the gap between the storage tube (9) and the extraction tube (2).

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