Large-view-field high-resolution microscopic module

Abstract

The invention discloses a large-view-field high-resolution microscopic module which comprises a supporting plate, an imaging assembly located at the top of the supporting plate, a dual-mode imaging switching assembly and a light source output assembly which are located at the bottom of the supporting plate, and an observation module located below the dual-mode imaging switching assembly. The dual-mode imaging switching assembly comprises an adjusting bin, a bright field reflection module and a fluorescence excitation module, wherein the bright field reflection module and the fluorescence excitation module are movably arranged in the adjusting bin. The adjusting bin is provided with an emergent hole communicated with the imaging assembly, a light hole communicated with the light source output assembly and an observation hole communicated with the observation module. According to the arrangement, the bright field reflection module and the fluorescence excitation module are moved to the light path communication position when needed, so that switching of imaging modes can be achieved, the switching structure is simple and easy to operate, and the number of imaging channels can be effectively guaranteed; and the clear aperture of the switching mechanism can be flexibly configured according to the pupil diameter of the objective lens, so that the effects of large view field and high-resolution imaging are achieved, and the application scene is wider.

Description

Technical Field

[0001] This invention relates to the field of microscopic imaging technology, and more specifically to a large field-of-view, high-resolution microscopic module. Background Technology

[0002] The field of view and resolution are two core performance indicators of a microscope, which together determine the microscope's observation capabilities. The field of view refers to the actual area within the circular field of view under a microscope that can accommodate the object being examined; the field number (FN) is an eyepiece parameter calibrated by the microscope manufacturer based on the characteristics of the optical system, representing the diameter (in millimeters) of the central image plane region that can be observed through the eyepiece.

[0003] Microscopic imaging resolution is a core indicator of microscope performance, determining its ability to observe microscopic details. Microscopic imaging resolution refers to the minimum distance between two adjacent points on a sample that a microscope can clearly distinguish; it is also known as resolving power.

[0004] There is an inherent contradiction between the field of view and resolution in traditional microscopes. For example, when a low-power microscope is needed to observe a large area, resolution must be sacrificed; when a high-power microscope is needed to observe details, the field of view will be sacrificed.

[0005] Existing microscopic equipment, such as scanning stitching and multi-camera / lens arrays, while taking into account the field of view and resolution of microscopic equipment, has significant limitations such as slow speed, high complexity, and high cost. Summary of the Invention

[0006] Therefore, the present invention aims to solve the problems of slow speed, high complexity, and high cost of existing microscopy devices that balance field of view and resolution, and thus provides a large field of view, high resolution microscopy module.

[0007] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows: A large field-of-view, high-resolution microscope module includes: A support plate, one end of which has a through hole; An imaging component is disposed on the top of the support plate, and one end of it communicates with the through hole; A dual-mode imaging switching component includes an adjustment chamber connected to the bottom of the support plate, a bright-field reflection module and a fluorescence excitation module movably disposed within the adjustment chamber. The adjustment chamber has an exit hole near the top of the support plate, communicating with the through hole, and an observation hole coaxially arranged with the through hole and the exit hole at its bottom away from the support plate. A light-transmitting hole is provided on the side wall of the adjustment chamber, adapted to be perpendicular to the axis of the observation hole. The bright-field reflection module and the fluorescence excitation module are adapted to move independently to the positions of the observation hole and the light-transmitting hole. Both the bright-field reflection module and the fluorescence excitation module are provided with an incident light path and an emitted light path. A light source output component is disposed at the bottom of the support plate, with one end connected to the light-transmitting hole, which is suitable for providing uniform illumination light; An observation module is located below the adjustment chamber and connected to the observation hole. The observation module is adapted to receive the illumination light from the light source output component and reflect it to the imaging component through the bright field reflection module or the fluorescence excitation module.

[0008] Furthermore, the bright-field reflection module includes a first movable box body movably connected to the adjustment chamber, a semi-transparent and semi-reflective beam splitter movably disposed in the first movable box body, and a first pressure cover connected to the semi-transparent and semi-reflective beam splitter. The semi-transparent and semi-reflective beam splitter is connected to the first movable box body at an angle of 45°.

[0009] Furthermore, the fluorescence excitation module includes a second movable housing movably connected to the adjustment chamber, a multi-channel excitation light filter disposed within the second movable housing, and a multi-channel dichroic mirror movably disposed within the second movable housing, wherein the multi-channel dichroic mirror is connected to the second movable housing at an angle of 45°.

[0010] Furthermore, both the first and second movable boxes are cuboid structures. The first movable box has three side walls respectively provided with a first circular hole suitable for connecting to the through hole, a second circular hole suitable for connecting to the light-transmitting hole, and a third circular hole suitable for connecting to the observation hole. The second movable box has three side walls respectively provided with a fourth circular hole suitable for connecting to the through hole, a fifth circular hole suitable for connecting to the light-transmitting hole, and a sixth circular hole suitable for connecting to the observation hole. The multi-channel excitation filter is fixedly installed in the fifth circular hole through a filter lens mount. A filter retaining ring covering the multi-channel excitation filter is also provided on the side of the fifth circular hole away from the interior of the second movable box.

[0011] Furthermore, the bottom of the adjustment chamber is provided with guide rails located on opposite sides of the observation hole, and the bright-field reflection module and the fluorescence excitation module are both slidably connected to the guide rails; a first pull rod extending out of the adjustment chamber is provided on the side wall of the bright-field reflection module away from the fluorescence excitation module, and a second pull rod extending out of the adjustment chamber is provided on the side wall of the fluorescence excitation module away from the bright-field reflection module.

[0012] Furthermore, both the bright-field reflection adjustment module and the fluorescence excitation module are connected to the guide rail via sliders; a spring plunger is provided on the side of the slider near the inner wall of the adjustment chamber, and a positioning groove adapted to the spring plunger is provided on the inner wall of the adjustment chamber.

[0013] Furthermore, the light source output component includes a beam shaping module connected to the light-transmitting aperture and an optical fiber connector disposed at the end of the beam shaping module away from the light-transmitting aperture. The beam shaping module is adapted to convert the light beam generated by the light source into uniform illumination light, and the optical fiber connector is electrically connected to a multi-channel LED light source.

[0014] Furthermore, a reflective component is provided on the through hole to connect to the imaging component. The reflective component includes a mirror mount fixedly connected to the support plate and a mirror connected to the mirror mount. The first side of the mirror mount is connected to the through hole, and the vertically adjacent second side is connected to the imaging component. The mirror is connected at an angle of 45° to the first side and the second side of the mirror mount.

[0015] Furthermore, the imaging assembly includes a tube mirror, a light shield, and a large target camera connected in sequence, with the tube mirror connected to the mirror mount; an adjustment platform is provided at one end of the support plate away from the through hole, the large target camera is threadedly connected to the adjustment platform, and the adjustment platform is adapted to extend along the length direction of the support plate.

[0016] Furthermore, the observation module is a large field-of-view mesoscopic objective lens that is threadedly connected to the observation hole.

[0017] The technical solution of this invention has the following advantages: 1. The large field-of-view, high-resolution microscope module provided by this invention forms an observation channel by opening an observation hole at the bottom of the adjustment chamber to connect with the observation module; a dual-mode imaging switching component is placed between the light source output module, the observation module, and the imaging component; an optical path is formed by opening a light-transmitting hole in the side wall of the adjustment chamber to connect with the light source output module; an imaging channel is formed by opening a through hole at the end of the support plate to connect the adjustment chamber and the imaging component; since the bright-field reflection module and the fluorescence excitation module are movably disposed within the adjustment chamber and can both be moved to the positions of the observation hole and the light-transmitting hole; when bright-field imaging is required, the bright-field reflection module is moved to the position of the observation hole, and the required light beam is provided through the light source output module, with the light beam passing through the optical path. Upon entering the bright-field reflection module, the light beam, after passing through the observation module, forms illumination light on the sample under test. The observation module then transmits the observed image data through the bright-field reflection module and out through the exit aperture to the imaging component. The imaging component receives the image data and transmits it to the computer to form a digital image. When fluorescence imaging is required, the fluorescence excitation module is moved to the position of the observation aperture, and the required light beam is provided by the light source output module. The light beam enters the fluorescence excitation module through the optical path, and after passing through the observation module, forms illumination light on the sample under test. The observation module then transmits the observed image data through the fluorescence excitation module and out through the exit aperture to the imaging component. The imaging component receives the image data and transmits it to the computer to form a digital image. This configuration, with movable bright-field reflection and fluorescence excitation modules located within the adjustment chamber, allows for switching of imaging modes by moving them to positions where the optical path is connected when needed. The switching structure is simple and easy to operate, effectively ensuring the number of imaging channels. Furthermore, the aperture of the switching mechanism can be flexibly configured according to the objective lens pupil diameter, achieving both a large field of view and high-resolution imaging, thus broadening the application scenarios.

[0018] 2. The large field-of-view, high-resolution microscope module provided by this invention includes a bright-field reflection module comprising a first movable housing movably connected to the adjustment chamber, a semi-transparent, semi-reflective beam splitter movably disposed within the first movable housing, and a first pressure cover connected to the semi-transparent, semi-reflective beam splitter. The semi-transparent, semi-reflective beam splitter is connected to the first movable housing at a 45° angle. This configuration allows for precise light distribution and optimized image quality through the semi-transparent, semi-reflective beam splitter, preventing direct illumination from interfering with observation results. The pressure cover isolates stray light when using the bright-field reflection module.

[0019] 3. The large field-of-view, high-resolution microscope module provided by this invention includes a fluorescence excitation module comprising a second movable housing movably connected to the adjustment chamber, a multi-channel excitation light filter disposed within the second movable housing, and a multi-channel dichroic mirror movably disposed within the second movable housing, the multi-channel dichroic mirror being obliquely connected to the second movable housing at a 45° angle. This configuration allows for the selection of the desired wavelength of excitation light via the multi-channel excitation light filter and the selection of the desired wavelength band via the multi-channel dichroic mirror, thereby acquiring a multi-channel fluorescence image; the pressure cap can isolate stray light when using the fluorescence excitation module.

[0020] 4. The large field-of-view, high-resolution microscope module provided by this invention has guide rails located on opposite sides of the observation aperture at the bottom of the adjustment chamber. Both the bright-field reflection module and the fluorescence excitation module are slidably connected to the guide rails. A first pull rod extending out of the adjustment chamber is provided on the side wall of the bright-field reflection module away from the fluorescence excitation module, and a second pull rod extending out of the adjustment chamber is provided on the side wall of the fluorescence excitation module away from the bright-field reflection module. This configuration, by using guide rails, improves the smoothness of movement and adjustment speed of the bright-field reflection module and the fluorescence excitation module; by using pull rods, the positions of the bright-field reflection module and the fluorescence excitation module can be adjusted more quickly and conveniently.

[0021] 5. The large field-of-view, high-resolution microscope module provided by this invention has both a bright-field reflection module and a fluorescence excitation module connected to a guide rail via sliders. A spring plunger is provided on the side of the slider near the inner wall of the adjustment chamber, and a positioning groove adapted to the spring plunger is provided on the inner wall of the adjustment chamber. This configuration allows the bright-field reflection module or fluorescence excitation module to be positioned after it has been moved into place, and then positioned by the cooperation of the spring plunger and the positioning groove. The cooperation of the spring plunger and the positioning groove effectively ensures the accuracy of the alignment of the bright-field reflection module or fluorescence excitation module with the optical path during adjustment, thereby ensuring the accuracy of the subsequent observation and imaging data.

[0022] 6. The large field-of-view, high-resolution microscopy module provided by this invention includes a light source output component comprising a beam shaping module connected to a light-transmitting aperture and an optical fiber connector disposed at the end of the beam shaping module away from the light-transmitting aperture. The beam shaping module is adapted to convert the light beam generated by the light source into uniform illumination light, and the optical fiber connector is electrically connected to a multi-channel LED light source. This configuration can effectively reduce the cost of using the light source.

[0023] 7. The large field-of-view, high-resolution microscope module provided by this invention includes an imaging component comprising a tube mirror, a light-shielding tube, and a large target-surface camera connected in sequence. The tube mirror is connected to a mirror mount. An adjustment platform is provided at the end of the support plate away from the through hole, and the large target-surface camera is threadedly connected to the adjustment platform. The adjustment platform is adapted to extend along the length of the support plate. This arrangement facilitates the adjustment of the target surface of the large-surface camera to a preset position via the adjustment platform, thereby better receiving the light signal from the tube mirror and converting it into a digital signal for transmission to the computer to form an accurate digital image.

[0024] 8. The large field-of-view, high-resolution microscopy module provided by this invention has an observation module that is a large field-of-view mesoscopic objective lens threadedly connected to the observation aperture. This configuration allows for the rapid acquisition of large-area images through the large field-of-view mesoscopic objective lens, simultaneously achieving high-resolution and ultra-large imaging field of view microscopy, thereby significantly improving work efficiency. Attached Figure Description

[0025] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0026] Figure 1 A three-dimensional structural diagram of the large field-of-view, high-resolution microscope module provided by the present invention; Figure 2 A three-dimensional structural diagram of the large field-of-view, high-resolution microscope module provided by the present invention; Figure 3 This is a three-dimensional structural diagram of the dual-mode imaging switching component in this invention; Figure 4 This is a three-dimensional structural diagram of the bright-field reflection module in this invention; Figure 5 This is an exploded view of the bright-field reflection module in this invention; Figure 6 This is a three-dimensional structural diagram of the fluorescence excitation module in this invention; Figure 7 This is an exploded view of the fluorescence excitation module in this invention; Explanation of reference numerals in the attached figures: 1. Support plate; 11. Positioning slot; 2. Adjustment chamber; 21. Spring plunger; 22. Light transmission hole; 23. Observation hole; 24. Guide rail; 25. Base plate; 26. Side plate; 3. Bright-field reflection module; 31. First movable housing; 32. Semi-transparent and semi-reflective beam splitter; 33. First pressure cover; 34. First mirror mount; 35. First circular hole; 36. Second circular hole; 37. First pull rod; 4. Fluorescence excitation module; 41. Second movable housing; 42. Multi-channel dichroic mirror; 43. Second pressure cover; 44. 45. Second mirror mount; 46. Multi-channel excitation filter; 47. Filter mount; 48. Filter retainer ring; 49. Fourth circular hole; 40. Fifth circular hole; 410. Second pull rod; 5. Imaging assembly; 51. Tube mirror; 52. Light shield; 53. Large target camera; 54. Adjustment stage; 6. Light source output assembly; 61. Beam shaping module; 62. Fiber optic connector; 63. Multi-channel LED light source; 7. Observation module; 8. Reflection assembly; 81. Reflector mount; 82. Reflector; 9. Slider. Detailed Implementation

[0027] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0029] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0030] Furthermore, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0031] like Figures 1-7The large field-of-view, high-resolution microscope module shown includes a support plate 1, an imaging component 5 mounted on the top of the support plate 1, a dual-mode imaging switching component and a light source output component 6 mounted on the bottom of the support plate 1, and an observation module 7 mounted on the bottom of the dual-mode imaging switching component. Specifically, the support plate 1 has a through hole at one end; the imaging component 5 is located on the top of the support plate 1, with one end communicating with the through hole; the dual-mode imaging switching component includes an adjustment chamber 2 connected to the bottom of the support plate 1, a bright-field reflection module 3 and a fluorescence excitation module 4 movably disposed within the adjustment chamber 2, an exit hole communicating with the through hole being opened on the top of the adjustment chamber 2 near the support plate 1, and an observation hole 23 coaxially arranged with the through hole and the exit hole being opened on the bottom away from the support plate 1; a light-transmitting hole 22 suitable for perpendicularly intersecting the axis of the observation hole 23 is opened on the side wall of the adjustment chamber 2. The bright-field reflection module 3 and the fluorescence excitation module 4 are adapted to be moved independently to the positions of the observation hole 23 and the light transmission hole 22. Both the bright-field reflection module 3 and the fluorescence excitation module 4 are provided with an incident light path and an emitted light path. The light source output component 6 is located at the bottom of the support plate 1, with one end connected to the light transmission hole 22, and is adapted to provide uniform illumination light. The observation module 7 is located below the adjustment chamber 2 and is connected to the observation hole 23. The observation module 7 is adapted to receive the illumination light from the light source output component 6 and reflect it to the imaging component 5 through the bright-field reflection module 3 or the fluorescence excitation module 4.

[0032] This large field-of-view, high-resolution microscope module can form an observation channel by opening an observation hole 23 at the bottom of the adjustment chamber 2 to connect with the observation module 7; a dual-mode imaging switching component can be placed between the light source output module, the observation module 7, and the imaging component 5, and an optical path can be formed by opening a light-transmitting hole 22 on the side wall of the adjustment chamber 2 to connect with the light source output module; an imaging channel can be formed by opening a through hole at the end of the support plate 1 to connect the adjustment chamber 2 and the imaging component 5; since the bright-field reflection module 3 and the fluorescence excitation module 4 are movably disposed within the adjustment chamber 2 and can both be moved to the positions of the observation hole 23 and the light-transmitting hole 22; when bright-field imaging is required, by moving the bright-field reflection module 3 to the position of the observation hole 23, the required beam is provided through the light source output module, and the beam passes through the optical path Upon entering the bright-field reflection module 3, the light beam, after passing through the observation module 7, forms illumination light to irradiate the sample under test. The observation module 7 then transmits the observed image data through the bright-field reflection module 3 and then through the exit aperture to the imaging component 5. The imaging component 5 receives the image data and transmits it to the computer to form a digital image. When fluorescence imaging is required, the fluorescence excitation module 4 is moved to the position of the observation aperture 23, and the required light beam is provided through the light source output module. The light beam enters the fluorescence excitation module 4 through the optical path, and after passing through the observation module 7, it forms illumination light to irradiate the sample under test. The observation module 7 then transmits the observed image data through the fluorescence excitation module 4 and then through the exit aperture to the imaging component 5. The imaging component 5 receives the image data and transmits it to the computer to form a digital image. With this configuration, the movable bright-field reflection module 3 and fluorescence excitation module 4, located within the adjustment chamber 2, can be moved to a position where the optical path is connected when needed to switch imaging modes. The switching structure is simple and easy to operate, effectively ensuring the number of imaging channels. Furthermore, the aperture of the switching mechanism can be flexibly configured according to the diameter of the objective lens pupil, achieving both a large field of view and high-resolution imaging, thus broadening the application scenarios.

[0033] In this embodiment, the bright-field reflection module 3 includes a first movable housing 31 movably connected to the adjustment chamber 2, a semi-transparent and semi-reflective beam splitter 32 movably disposed within the first movable housing 31, and a first pressure cover 33 connected to the semi-transparent and semi-reflective beam splitter 32. The semi-transparent and semi-reflective beam splitter 32 is connected to the first movable housing 31 at a 45° angle. This configuration allows for precise light distribution and optimized image quality through the semi-transparent and semi-reflective beam splitter 32, while avoiding interference from direct illumination by the light source.

[0034] In this embodiment, the fluorescence excitation module 4 includes a second movable housing 41 movably connected to the adjustment chamber 2, a multi-channel excitation light filter disposed within the second movable housing 41, and a multi-channel dichroic mirror 42 movably disposed within the second movable housing 41. The multi-channel dichroic mirror 42 is connected to the second movable housing 41 at a 45° angle. With this configuration, the desired wavelength of excitation light is selected by the multi-channel excitation light filter, and the desired wavelength band is selected by the multi-channel dichroic mirror 42, thereby acquiring a multi-channel fluorescence image.

[0035] In this embodiment, both the first movable box 31 and the second movable box 41 are cuboid structures. The three side walls of the first movable box 31 are respectively provided with a first circular hole 35 suitable for connecting to the through hole, a second circular hole 36 suitable for connecting to the light-transmitting hole 22, and a third circular hole suitable for connecting to the observation hole 23. The three side walls of the second movable box 41 are respectively provided with a fourth circular hole 48 suitable for connecting to the through hole, a fifth circular hole 49 suitable for connecting to the light-transmitting hole 22, and a sixth circular hole suitable for connecting to the observation hole 23. The multi-channel excitation filter 45 is fixedly installed in the fifth circular hole 49 through the filter lens mount 46. A filter retaining ring 47 covering the multi-channel excitation filter 45 is also provided on the side of the fifth circular hole 49 away from the interior of the second movable box 41.

[0036] In this embodiment, the first movable housing 31 has 45° inclined grooves on both opposite side walls. A first pressure cover 33 is provided on one side of the inclined groove, and a frame-shaped first mirror mount 34 is movably disposed on the other side of the inclined groove. A semi-transparent and semi-reflective beam splitter 32 is fixedly connected to the first mirror mount 34. Specifically, the second movable housing 41 has 45° inclined grooves on both opposite side walls. A second pressure cover 43 is fixedly disposed on one side of the inclined groove, and a frame-shaped second mirror mount 44 is movably disposed on the other side of the inclined groove. A multi-channel dichroic mirror 42 is fixedly connected to the second mirror mount 44. With this configuration, the first pressure cover 33 can isolate stray light when using the bright field reflection module 3, and the second pressure cover 43 can isolate stray light when using the fluorescence excitation module 4.

[0037] In this embodiment, the adjustment chamber 2 includes a base plate 25 and side plates 26 connected to opposite sides of the base plate 25, with the opposite ends of the adjustment chamber 2 being through-through. Guide rails 24 are provided on the inner side of the base plate 25, located on opposite sides of the observation hole 23. Both the bright-field reflection module 3 and the fluorescence excitation module 4 are slidably connected to the guide rails 24. A first pull rod 37 extending out of the adjustment chamber 2 is provided on the side wall of the bright-field reflection module 3 away from the fluorescence excitation module 4, and a second pull rod 410 extending out of the adjustment chamber 2 is provided on the side wall of the fluorescence excitation module 4 away from the bright-field reflection module 3. This configuration improves the smoothness of movement and adjustment speed of the bright-field reflection module 3 and the fluorescence excitation module 4 by using the guide rails 24; and allows for faster and more convenient adjustment of the positions of the bright-field reflection module 3 and the fluorescence excitation module 4 by using the pull rods.

[0038] In this embodiment, both the bright-field reflection module 3 and the fluorescence excitation module 4 are connected to the guide rail 24 via a slider 9. A spring plunger 21 is provided on the side of the slider 9 near the inner wall of the adjustment chamber 2, and a positioning groove 11 adapted to the spring plunger 21 is provided on the inner wall of the adjustment chamber 2. With this configuration, after the bright-field reflection module 3 or the fluorescence excitation module 4 is moved into position, it is positioned by the cooperation of the spring plunger 21 and the positioning groove 11. The cooperation of the spring plunger 21 and the positioning groove 11 can effectively ensure the accuracy of the alignment of the bright-field reflection module 3 or the fluorescence excitation module 4 with the optical path during adjustment, thereby ensuring the accuracy of the subsequent observation and imaging data.

[0039] In this embodiment, the light source output component 6 includes a beam shaping module 61 connected to the light-transmitting aperture 22 and an optical fiber connector 62 disposed at the end of the beam shaping module 61 away from the light-transmitting aperture 22. The beam shaping module 61 is adapted to convert the light beam generated by the light source into uniform illumination light, and the optical fiber connector 62 is electrically connected to a multi-channel LED light source 63. This configuration can effectively reduce the cost of using the light source.

[0040] In this embodiment, a reflective component 8 connected to the imaging component 5 is provided on the through hole. The reflective component 8 includes a mirror mount 81 fixedly connected to the support plate 1 and a mirror 82 connected to the mirror mount 81. The first side of the mirror mount 81 is connected to the through hole, and the vertically adjacent second side is connected to the imaging component 5. The mirror 82 is obliquely connected at 45° to the first and second sides of the mirror mount 81. Specifically, the mirror 82 is movably connected to the mirror mount 81, and the mirror 82 can be adjusted in two dimensions, tilt and pitch, through the mirror mount 81.

[0041] In this embodiment, the imaging assembly 5 includes a tube mirror 51, a light-shielding tube 52, and a large target-surface camera 53 connected in sequence. The tube mirror 51 is connected to the mirror mount 81. An adjustment platform 54 is provided at the end of the support plate 1 away from the through hole. The large target-surface camera 53 is threadedly connected to the adjustment platform 54, which is adapted to extend along the length of the support plate 1. With this configuration, the tube mirror 51 receives the collimated beam from the observation module 7 and the mirror 82 and focuses it onto the large target-surface camera 53. The target surface of the large target-surface camera is adjusted to a preset position by the adjustment platform 54, which can better receive the light signal from the tube mirror 51 and convert it into a digital signal before transmitting it to the computer to form an accurate digital image. Specifically, the focal length of the tube mirror 51 is 400mm, and the large target-surface camera has the following parameters: Target dimensions: 52.43 × 29.44 mm (60.13 mm diagonally); Resolution: 16384×9200 pixels; Pixel size: 3.2×3.2um; Imaging frame rate: 16fps@16384×9200pixels.

[0042] In this embodiment, the observation module 7 is a large field-of-view mesoscopic objective lens threadedly connected to the observation hole 23. This configuration allows for the rapid acquisition of large-area images through the large field-of-view mesoscopic objective lens, while simultaneously achieving high-resolution and ultra-large imaging field of view microscopic imaging, thereby significantly improving work efficiency.

[0043] Specifically, a large field-of-view mesoscopic objective lens is used to transmit an illumination beam while simultaneously collecting scattered light or fluorescence and converting it into a collimated beam. In one feasible embodiment, the large field-of-view mesoscopic objective lens has the following parameters: With a numerical aperture (NA) of 0.5, it is suitable for general microscopic observation and provides moderate resolution and light throughput. The object-side field of view (FOV) is Φ6mm, and a single observation can cover a circular area with a diameter of 6mm, which is suitable for observing larger sample areas; The focal length (f) is 40mm; The operating wavelength (λ) is 400~800nm, which covers the entire visible light spectrum (violet light 400nm to red light 800nm).

[0044] The above parameters can effectively balance the requirements of resolution and multicolor imaging with a large field-of-view mesoscopic objective lens.

[0045] In one feasible embodiment, multi-channel fluorescence images can be acquired by setting the following parameters:

[0046] In this embodiment, multi-channel bright-field grayscale images can be obtained by setting the parameters as described in the table above.

[0047] Specifically, image fusion algorithms can be used to combine bright-field grayscale images from the R / G / B channels into a bright-field color image.

[0048] In this embodiment, the large field-of-view, high-resolution microscope module has the following performance parameters: Imaging field of view (non-stitched): 5.24×2.94mm (6.0mm diagonally); that is, forming an observation area of ​​5.24mm horizontally and 2.94mm vertically, and the complete field of view can be obtained in a single exposure without the need for image stitching technology; Spatial resolution: 0.64 μm; this resolution allows for clear observation of mesoscale features of the sample. Magnification: 10X; Imaging speed: 16fps@16384×9200 pixels; This imaging speed allows for capturing detailed images of moving samples at high speeds. Imaging mode: Fluorescence imaging / Bright field imaging; Imaging channel count: ≥4; can simultaneously observe at least 4 different fluorescently labeled samples, suitable for multicolor experiments.

[0049] The large field-of-view, high-resolution microscope module of this application significantly improves performance parameters such as imaging field of view, spatial resolution, and imaging speed, achieving both large field of view and high-resolution rapid imaging. It also offers more imaging modes and channels, broadening its application scenarios. Compared to traditional rotary fluorescence switching modules, the dual-mode imaging switching component provided in this application uses only one set of fluorescence excitation modules 4 and one set of bright-field reflection modules 3 to achieve both fluorescence and bright-field imaging modes and multi-channel imaging. Furthermore, the use of the reflector 82 simplifies and compacts the overall structure of the large field-of-view, high-resolution microscope module, allowing for easier integration into other instruments.

[0050] In summary, this large field-of-view, high-resolution microscope module can form an observation channel by opening an observation hole 23 at the bottom of the adjustment chamber 2 to connect with the observation module 7; by placing the dual-mode imaging switching component between the light source output module, the observation module 7, and the imaging component 5, and by opening a light-transmitting hole 22 on the side wall of the adjustment chamber 2 to connect with the light source output module, an optical path can be formed; and by opening a through hole at the end of the support plate 1 to connect the adjustment chamber 2 and the imaging component 5, an imaging channel can be formed. Since the bright-field reflection module 3 and the fluorescence excitation module 4 are movably disposed within the adjustment chamber 2 and can both be moved to the positions of the observation hole 23 and the light-transmitting hole 22, when bright-field imaging is required, the bright-field reflection module 3 is moved to the position of the observation hole 23, and the required beam is provided through the light source output module. The light beam enters the bright-field reflection module 3 through the optical path. After passing through the observation module 7, the light beam forms illumination light to illuminate the sample under test. The observation module 7 then transmits the observed image data through the bright-field reflection module 3 and then through the exit aperture to the imaging component 5. The imaging component 5 receives the image data and transmits it to the computer to form a digital image. When fluorescence imaging is required, the fluorescence excitation module 4 is moved to the position of the observation aperture 23, and the required light beam is provided through the light source output module. The light beam enters the fluorescence excitation module 4 through the optical path. After passing through the observation module 7, the light beam forms illumination light to illuminate the sample under test. The observation module 7 then transmits the observed image data through the fluorescence excitation module 4 and then through the exit aperture to the imaging component 5. The imaging component 5 receives the image data and transmits it to the computer to form a digital image. With this configuration, the movable bright-field reflection module 3 and fluorescence excitation module 4, located within the adjustment chamber 2, can be moved to a position where the optical path is connected when needed to switch imaging modes. The switching structure is simple and easy to operate, effectively ensuring the number of imaging channels. Furthermore, the aperture of the switching mechanism can be flexibly configured according to the diameter of the objective lens pupil, achieving both a large field of view and high-resolution imaging, thus broadening the application scenarios.

[0051] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A large field-of-view, high-resolution microscope module, characterized in that, include: The support plate (1) has a through hole at one end; An imaging component (5) is disposed on the top of the support plate (1), and one end of it is connected to the through hole; The dual-mode imaging switching assembly includes an adjustment chamber (2) connected to the bottom of the support plate (1), a bright-field reflection module (3) and a fluorescence excitation module (4) movably disposed within the adjustment chamber (2). The adjustment chamber (2) has an exit hole connected to the through hole at its top near the support plate (1) and an observation hole (23) coaxially disposed with the through hole and the exit hole at its bottom away from the support plate (1). The side wall of the adjustment chamber (2) has a light-transmitting hole (22) adapted to be perpendicular to the axis of the observation hole (23). The bright-field reflection module (3) and the fluorescence excitation module (4) are adapted to be moved independently to the positions of the observation hole (23) and the light-transmitting hole (22). Both the bright-field reflection module (3) and the fluorescence excitation module (4) are provided with an incident light path and an emitted light path. A light source output component (6) is disposed at the bottom of the support plate (1), with one end connected to the light-transmitting hole (22), and is suitable for providing uniform illumination light; An observation module (7) is located below the adjustment chamber (2) and connected to the observation hole (23). The observation module (7) is adapted to receive the illumination light from the light source output component (6) and reflect it to the imaging component (5) through the bright field reflection module (3) or the fluorescence excitation module (4).

2. The large field-of-view, high-resolution microscope module according to claim 1, characterized in that, The bright field reflection module (3) includes a first movable box (31) movably connected to the adjustment chamber (2), a semi-transparent and semi-reflective beam splitter (32) movably disposed in the first movable box (31), and a first pressure cover (33) connected to the semi-transparent and semi-reflective beam splitter (32). The semi-transparent and semi-reflective beam splitter (32) is connected to the first movable box (31) at an angle of 45°.

3. The large field-of-view, high-resolution microscope module according to claim 2, characterized in that, The fluorescence excitation module (4) includes a second movable box (41) movably connected to the adjustment chamber (2), a multi-channel excitation light filter disposed in the second movable box (41), and a multi-channel dichroic mirror (42) movably disposed in the second movable box (41). The multi-channel dichroic mirror (42) is connected to the second movable box (41) at an angle of 45°.

4. The large field-of-view, high-resolution microscope module according to claim 3, characterized in that, Both the first movable box (31) and the second movable box (41) are cuboid structures. The first movable box (31) has three side walls respectively provided with a first circular hole (35) suitable for connecting to the through hole, a second circular hole (36) suitable for connecting to the light-transmitting hole (22), and a third circular hole suitable for connecting to the observation hole (23). The second movable box (41) has three side walls respectively provided with a fourth circular hole (48) suitable for connecting to the through hole, a fifth circular hole (49) suitable for connecting to the light-transmitting hole (22), and a sixth circular hole suitable for connecting to the observation hole (23). The multi-channel excitation filter (45) is fixedly installed in the fifth circular hole (49) through the filter lens mount (46). The side of the fifth circular hole (49) away from the interior of the second movable box (41) is also provided with a filter retaining ring (47) covering the multi-channel excitation filter (45).

5. The large field-of-view, high-resolution microscope module according to claim 1, characterized in that, The bottom of the adjustment chamber (2) is provided with guide rails (24) located on opposite sides of the observation hole (23). The bright field reflection module (3) and the fluorescence excitation module (4) are slidably connected to the guide rails (24). A first pull rod (37) extending out of the adjustment chamber (2) is provided on the side wall of the bright field reflection module (3) away from the fluorescence excitation module (4). A second pull rod (410) extending out of the adjustment chamber (2) is provided on the side wall of the fluorescence excitation module (4) away from the bright field reflection module (3).

6. The large field-of-view, high-resolution microscope module according to claim 5, characterized in that, The bright field reflection module (3) and the fluorescence excitation module (4) are both connected to the guide rail (24) via a slider (9); a spring plunger (21) is provided on the side of the slider (9) near the inner wall of the adjustment chamber (2), and a positioning groove (11) adapted to the spring plunger (21) is provided on the inner wall of the adjustment chamber (2).

7. The large field-of-view, high-resolution microscope module according to claim 1, characterized in that, The light source output component (6) includes a beam shaping module (61) connected to the light-transmitting hole (22) and an optical fiber connector (62) disposed at the end of the beam shaping module (61) away from the light-transmitting hole (22). The beam shaping module (61) is adapted to convert the light beam generated by the light source into uniform illumination light. The optical fiber connector (62) is electrically connected to a multi-channel LED light source (63).

8. The large field-of-view, high-resolution microscope module according to claim 1, characterized in that, A reflective component (8) is provided on the through hole to connect to the imaging component (5). The reflective component (8) includes a mirror mount (81) fixedly connected to the support plate (1) and a mirror (82) connected to the mirror mount (81). The first side of the mirror mount (81) is connected to the through hole, and the second side, which is perpendicularly adjacent to it, is connected to the imaging component (5). The mirror (82) is connected at an angle of 45° to the first side and the second side of the mirror mount (81).

9. The large field-of-view, high-resolution microscope module according to claim 8, characterized in that, The imaging assembly (5) includes a tube mirror (51), a light shield (52), and a large target camera (53) connected in sequence. The tube mirror (51) is connected to the mirror mount (81). An adjustment platform (54) is provided on one end of the support plate (1) away from the through hole. The large target camera (53) is threadedly connected to the adjustment platform (54). The adjustment platform (54) is adapted to extend along the length direction of the support plate (1).

10. The large field-of-view, high-resolution microscope module according to claim 1, characterized in that, The observation module (7) is a large field-of-view mesoscopic objective lens that is threadedly connected to the observation hole (23).

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