Preparation method of cordyceps medium using smilax bracteata as culture medium

By enzymatic hydrolysis and microencapsulation of Polygonatum yunnanense, combined with 3D printing to construct a layered Cordyceps culture medium, the problems of insufficient release of active ingredients and inadequate culture medium structure in existing technologies have been solved, achieving efficient Cordyceps cultivation and improved product quality.

CN120982351BActive Publication Date: 2026-07-24INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510992841.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-07-18
Publication Date
2026-07-24
Estimated Expiration
2045-07-18

AI Technical Summary

Technical Problem

Existing cordyceps culture media, when using complex plant materials such as Polygonatum yunnanense, have difficulty releasing active ingredients as needed, and the culture medium structure is difficult to meet the needs of different growth stages of cordyceps, resulting in insufficient functional design and integration.

Method used

By enzymatically hydrolyzing Polygonatum yunnanense, a hydrolysate rich in small molecule nutrients was prepared. The active components were then microencapsulated, and a layered Cordyceps culture medium was constructed using 3D printing technology to achieve targeted release and gradient nutrient supply.

Benefits of technology

It improved the cultivation efficiency and yield of Cordyceps, optimized the cultivation environment, shortened the cultivation cycle, increased the cordycepin content, and made the culture medium preparation process more standardized and controllable.

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Abstract

The application discloses a cordyceps culture medium preparation method using yunnan rhizoma polygonati as the culture medium, and relates to the technical field of culture medium preparation.The method comprises the following steps: subjecting yunnan rhizoma polygonati raw materials to enzymolysis treatment to obtain an enzymolysis solution rich in small-molecule nutrients; extracting active components of yunnan rhizoma polygonati to prepare microcapsules with a response release characteristic; respectively using the enzymolysis solution and components containing the microcapsules and yunnan rhizoma polygonati materials without enzymolysis to prepare at least two 3D bioprinting inks; and layer-by-layer constructing a three-dimensional culture medium with a layered or gradient structure by using a 3D printing technology, and performing solidification.The culture medium prepared by the method can realize gradient distribution of nutrients and programmed release of active components, and can provide an optimized microenvironment for growth of cordyceps, so that the culture efficiency, yield and quality of the cordyceps are effectively improved.
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Citation Information

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