Composition and cosmetic with anti-wrinkle and firming effects

Abstract

The invention discloses a composition with anti-wrinkle and firming effects and a cosmetic. In the recombinant collagen-calanthe extract composition with the anti-wrinkle and firming effects, the recombinant III type collagen and the APG tripeptide have a synergistic effect in the aspects of keeping skin elasticity and resisting wrinkles and firming; the recombinant I-type collagen and the LPG tripeptide have a synergistic effect in the aspects of increasing the thickness of a corium layer of the skin and promoting skin repair; and by combining the recombinant collagen component with the calanthe extract, a higher-level anti-wrinkle and firming effect can be realized, so that a more effective anti-wrinkle and firming product is provided for consumers.

Description

TECHNICAL FIELD

[0001] The present patent application belongs to the field of skin care cosmetics, and in particular, relates to a recombinant collagen-orchid extract composition with good anti-wrinkle and firming effects and a cosmetic using the composition as a raw material. BACKGROUND

[0002] With the improvement of people's living standards, there is an increasing demand for anti-wrinkle and firming effects of cosmetics. Collagen is an important component of the skin, and its reduction will lead to skin relaxation and wrinkle formation. Recombinant collagen, as a new source of collagen, has the advantages of high safety and strong biological activity. In particular, some small molecule peptides from collagen are more easily absorbed by the skin and can more effectively exert their biological activity.

[0003] On the other hand, Calanthe discolor, a rare plant in the Orchidaceae family, has various bioactive components, including phenanthraquinones, alkaloids, and phenolic derivatives, and has antioxidant, anti-inflammatory, and collagen synthesis-promoting effects. Efficacy tests have confirmed that the active ingredients in Calanthe discolor extract can induce adipose-derived stem cells to secrete a secretome rich in growth factors, which can stimulate fibroblasts to synthesize proteins and activate the regenerative capacity of fibroblasts, increasing the synthesis of various growth factors required for communication between fibroblasts and adipose-derived stem cells. Therefore, it can activate the signal communication between adipose-derived stem cells and fibroblasts, increase the synthesis of collagen and elastin, and thus improve skin firmness and elasticity.

[0004] In recent years, with the rapid development of the medical beauty industry, more and more functional recombinant collagens have been developed and applied in various cosmetics. However, how to combine recombinant collagen with other ingredients to better exert its cosmetic and skin care effects has not yet been paid attention to by researchers. SUMMARY

[0005] In view of the above technical problems in the prior art, the purpose of the present application is to provide a recombinant collagen composition that can cooperate with each other to exert anti-wrinkle and firming effects, and a cosmetic using the recombinant collagen composition as a raw material.

[0006] The inventors have conducted in-depth research on the synergies of various ingredients with anti-wrinkle firming efficacy, and found that: 1. A certain proportion of recombinant collagen type III described in Chinese patent application 201310701942.8 and APG tripeptide have synergistic effect in increasing fibroblast growth factor (b-FGF), thereby better maintaining and restoring skin elasticity, playing an anti-wrinkle firming role. 2. It is found for the first time that LPG tripeptide has the functions of increasing the thickness of the dermis layer of the skin and promoting skin repair, and a certain proportion of recombinant collagen type I described in Chinese patent application 201310701767.2 and LPG tripeptide have synergistic effect in increasing the thickness of the dermis layer of the skin and promoting skin repair. 3. By combining the above-mentioned recombinant collagen ingredients with shi jilan extract, a higher level of anti-wrinkle firming efficacy can be achieved, providing more effective anti-wrinkle firming cosmetics for consumers. The inventors have completed the present application based on the above scientific findings.

[0007] That is, the present application comprises:

[0008] 1. A recombinant collagen-shi jilan extract composition with anti-wrinkle firming efficacy, comprising, as active ingredients, recombinant collagen type III, recombinant collagen type I, APG tripeptide, LPG tripeptide and shi jilan extract, and a cosmetically acceptable carrier, wherein,

[0009] In the composition, the weight ratio of the recombinant collagen type III and the APG tripeptide is 250:1-5:1, and / or the weight ratio of the recombinant collagen type I and the LPG tripeptide is 250:1-5:1;

[0010] And the ratio of the sum of the weights of the recombinant collagen type III, the recombinant collagen type I, the APG tripeptide and the LPG tripeptide to the weight of the shi jilan extract is 700:1-35:1, preferably 400:1-50:1;

[0011] The amino acid sequence of the recombinant collagen type III is shown in SEQ ID No: 1;

[0012] The amino acid sequence of the recombinant collagen type I is shown in SEQ ID No: 2.

[0013] The shi jilan extract may, for example, be from Orchistem TM (Provital product).

[0014] 2. The recombinant collagen-shi jilan extract composition according to item 1, wherein the weight ratio of the recombinant collagen type III and the APG tripeptide is 25:1-5:1, and / or the weight ratio of the recombinant collagen type I and the LPG tripeptide is 25:1-5:1.

[0015] 3. A cosmetic comprising the recombinant collagen- Rhizoma Gastrodiae extract composition according to item 1 or 2, and one or more selected from a solvent, a humectant, a skin conditioner, an emulsifier, an emollient, a thickening agent, a pH adjuster.

[0016] 4. The cosmetic according to item 3, wherein the solvent is purified water.

[0017] 5. The cosmetic according to item 3, wherein the humectant is a combination of one or more selected from glycerin, 1,2-pentanediol, caprylyl glycol, and panthenol.

[0018] 6. The cosmetic according to item 3, wherein the skin conditioner is a combination of one or more selected from maltodextrin, sodium acetylated hyaluronate, Chlorella vulgaris extract, hydroxypropyl methylcellulose, and sunflower seed oil.

[0019] 7. The cosmetic according to item 3, wherein the emulsifier is one or more selected from cetostearyl alcohol, sorbitan oleate, arachidyl glucoside, and polysorbate-80.

[0020] 8. The cosmetic according to item 3, wherein the emollient is one or more selected from caprylic / capric triglyceride, dimethicone, butyrospermum parkii (shea) butter, squalane, and vaccinium myrtillus seed butter.

[0021] 9. The cosmetic according to item 3, wherein the thickening agent is one or more selected from carbomer, acrylates / C10-30 alkyl acrylate crosspolymer, cetostearyl alcohol, and chondrus crispus extract.

[0022] 10. The cosmetic according to item 3, wherein the pH adjuster is one or more selected from aminomethyl propanol, citric acid, sodium phosphate monobasic, and sodium phosphate dibasic.

[0023] 11. The cosmetic according to item 3, which is prepared in a form suitable for application to the skin. For example, it can be an aqueous solution, an emulsion, a cream, a pack, etc.

[0024] 12. A method for preparing the cosmetic according to item 3, comprising the following steps:

[0025] (1) adding a humectant, a thickening agent, collagen, an emollient, an emulsifier, and purified water to a main container, heating to dissolve at 80-85°C, and emulsifying until the material is uniform;

[0026] (2) starting to cool down to 40-45°C, adding a pH adjuster and part of a skin conditioner to the main container, and stirring until uniform;

[0027] (3) under stirring, the extract of Anoectochilus roxburghii (I) and the remaining skin conditioner are added into the main container in turn, and stirred uniformly; and

[0028] (4) cooled to 30℃ or below, and stirred uniformly, thus obtaining the cosmetic containing the recombinant collagen-Anoectochilus roxburghii extract composition.

[0029] 13. Use of the recombinant collagen-Anoectochilus roxburghii extract composition according to item 1 or 2 in the preparation of a cosmetic or a drug for anti-wrinkle firming, which is prepared in a form suitable for application to the skin.

[0030] 14. Use according to item 13, wherein the cosmetic is prepared in the form of a water agent, an emulsion, a cream or a mask.

[0031] Inventive Effects

[0032] In the recombinant collagen composition with anti-wrinkle firming effect described above, the recombinant collagen type III and the APG tripeptide have a synergistic effect in maintaining skin elasticity and anti-wrinkle firming; the recombinant collagen type I and the LPG tripeptide have a synergistic effect in increasing the thickness of the dermis layer of the skin and promoting skin repair; the addition of the extract of Anoectochilus roxburghii can achieve a higher level of anti-wrinkle firming effect; thus providing a more effective anti-wrinkle firming product for consumers.

[0033] DETAILED DESCRIPTION Embodiments

[0034] The embodiments given below are for the purpose of facilitating the understanding of the present application and do not in any way limit the scope of the claims of the present application.

[0035] The main materials used in the following embodiments include:

[0036] Recombinant collagen type III: prepared and purified according to the method disclosed in the specification of Chinese patent application 201310701942.8, and the amino acid sequence thereof is shown in SEQ ID No: 1.

[0037] SEQ ID No: 1

[0038]

[0039] Recombinant collagen type I: prepared and purified according to the method disclosed in the specification of Chinese patent application 201310701767.2, and its amino acid sequence is shown as SEQ ID No: 2.

[0040] SEQ ID No: 2

[0041]

[0042] Mini small molecule peptide-1: APG tripeptide (Ala-Pro-Gly), which was synthesized by a commercial company.

[0043] Mini small molecule peptide-2: LPG tripeptide (Leu-Pro-Gly), which was synthesized by a commercial company.

[0044] Example 1: Effect of each single component and composition on the amount of mouse fibroblast growth factor

[0045] The following reagents were prepared: type III recombinant collagen solution (25 mg / mL, aqueous solution), APG small molecule peptide solutions 1-5 (0.01 mg / mL, 0.1 mg / mL, 1 mg / mL, 5 mg / mL, 10 mg / mL, aqueous solution), compositions 1-5 (type III recombinant collagen 25 mg / mL + APG small molecule peptide 0.01 mg / mL, 0.1 mg / mL, 1 mg / mL, 5 mg / mL, 10 mg / mL, aqueous solution), commercially available recombinant collagen solution (25 mg / mL, aqueous solution), and purified water 100 mL.

[0046] The above solutions were used to group and apply to mice, and the specific steps were as follows:

[0047] After the mice were acclimated and fed for one week, 110 mice weighing 25-30 g were selected, and the remaining hair on the back of the mice was removed in an area of 1.0 x 1.0 cm. The mice were randomly divided into 11 groups, with 10 mice in each group. The purified water group served as the normal group, and the commercially available recombinant collagen solution served as the positive control group. The remaining groups were randomly divided into type III recombinant collagen group, APG small molecule collagen peptide-1 group (0.01 mg / mL), APG small molecule collagen peptide-2 group (0.1 mg / mL), APG small molecule collagen peptide-3 group (1 mg / mL), APG small molecule collagen peptide-4 group (5 mg / mL), APG small molecule collagen peptide-5 group (10 mg / mL), and composition 1-5 group. The mice were applied with the drugs twice a day, with a dosage of 0.2 mL / 10 g, for 4 weeks. Two hours after the last administration, the mice were sacrificed, and the back skin was cut and selected for tissue homogenate to perform ELISA detection to detect the amount of fibroblast growth factor (b-FGF). The b-FGF growth rate of each administration group was calculated.

[0048] b-FGF growth rate = b-FGF amount of administration group / b-FGF amount of normal group

[0049] The combined drug index CI = (A x B) / AB was calculated simultaneously, wherein A and B are the b-FGF growth rates of the type III recombinant collagen group and the APG small molecule collagen peptide 1-5 group respectively, and AB is the b-FGF growth rate of the composition 1-5 group. When CI < 1, it indicates that the combination has a synergistic effect; when CI ≤ 0.9, it indicates that the synergistic effect is significant. The effects of each single component and the composition on the amount of mouse fibroblast growth factor and the combined drug index are shown in Table 1.

[0050] Table 1

[0051] Group b-FGF (pg / mL) b-FGF growth rate CI Normal group 8.3±0.3 - / Positive control group 9.6±0.2 - / Type III recombinant collagen group 10.3±0.4 1.24 / APG-1 group 9.1±0.1 1.10 / APG-2 group 10.9±0.3 1.31 / APG-3 group 15.2±0.4 1.83 / APG-4 group 21.2±0.3 2.55 / APG-5 group 29.6±0.1 3.57 / Composition 1 group 10.5±0.4 1.27 1.08 Composition 2 group 14.7±0.1 1.77 0.92 Composition 3 group 23.6±0.1 2.84 0.80 Composition 4 group 30.4±0.6 3.66 0.87 Composition 5 group 36.1±0.3 4.35 1.02

[0052] As can be seen from Table 1, the compositions 1-5 of the present application can increase the amount of mouse fibroblast growth factor. At the same time, the combined drug indexes of the compositions 2-4 are all less than 1, indicating that the ratio of recombinant type III collagen to APG small molecule collagen peptide is 250:1-5:1, which has a synergistic effect. In particular, the combined drug index of the composition 3 is less than or equal to 0.8, indicating that the ratio of recombinant type III collagen to APG small molecule collagen peptide is 25:1-5:1, which has a significant synergistic effect. In the skin, type III recombinant collagen can promote the growth of fibroblasts and the secretion of collagen by fibroblasts, thereby maintaining and restoring skin elasticity and playing an anti-wrinkle and firming role. APG small molecule collagen peptide mainly acts as a type of elastin molecule and can promote the growth of dermal fibroblasts. Therefore, the combined effect of the two can greatly increase the number of dermal fibroblasts in the skin, achieving the effect of increasing skin elasticity and anti-wrinkle and firming.

[0053] Example 2: Effects of each single component and the composition on the thickness of mouse dermal layer

[0054] The following reagents were prepared: recombinant type I collagen solution (25 mg / mL, aqueous solution), LPG small molecule peptide solutions 1-5 (0.01 mg / mL, 0.1 mg / mL, 1 mg / mL, 5 mg / mL, 10 mg / mL, aqueous solution), compositions 1-5 (recombinant type I collagen 25 mg / mL + LPG small molecule peptide 0.01 mg / mL, 0.1 mg / mL, 1 mg / mL, 5 mg / mL, 10 mg / mL, aqueous solution), and purified water 100 mL.

[0055] The above solutions were used to group and administer the mice, and the specific steps were as follows:

[0056] After the mice were adapted to the feeding for one week, 130 mice with a weight of 25-30 g were selected, and the residual hair on the back of the mice was shaved in a region with a size of 1.0*1.0 cm. The mice were randomly divided into 13 groups, with 10 mice in each group. Purified water was used as the normal group, and 10% sodium lactate was used as the model group. The rest of the mice were randomly divided into the recombinant type I collagen group, the LPG small molecule collagen peptide-1 group (0.01 mg / mL), the LPG small molecule collagen peptide-2 group (0.1 mg / mL), the LPG small molecule collagen peptide-3 group (1 mg / mL), the LPG small molecule collagen peptide-4 group (5 mg / mL), the LPG small molecule collagen peptide-5 group (10 mg / mL), and the composition 1-5 groups after being treated with 10% sodium lactate for 7 days. The mice were administered with the drugs twice a day, and the administration amount was 0.2 mL / 10 g, which lasted for 4 weeks. Two hours after the last administration, the mice were sacrificed, and the skin on the back was cut and selected to prepare tissue sections. The dermal layer thickness was measured, and the dermal layer recovery coefficient of each administration group was calculated.

[0057] Dermal layer recovery coefficient = dermal layer thickness of the administration group / dermal layer thickness of the model group

[0058] The combination index CI = (A x B) / AB was also calculated, where A and B were the dermal layer recovery coefficients of the type I recombinant collagen group and the LPG small molecule peptide-1-5 group, respectively, and AB was the dermal layer recovery coefficient of the composition 1-5 group. When CI < 1, it indicated that the combination had a synergistic effect. When CI ≤ 0.9, it indicated that the synergistic effect was significant. The dermal layer recovery coefficients of each single component and the composition 1-5 and the combination index are shown in Table 2.

[0059] Table 2

[0060] Group Dermal layer thickness (μm) Dermal layer recovery coefficient CI Normal group 456±18 / / Model group 156±12 / / Recombinant type I collagen group 207±24 1.33 / LPG-1 group 169±7 1.08 / LPG-2 group 195±19 1.25 / LPG-3 group 242±34 1.55 / LPG-4 group 265±22 1.70 / LPG-5 group 331±27 2.12 / Composition 1 group 222±18 1.42 1.01 Composition 2 group 283±17 1.81 0.91 Composition 3 group 375±23 2.40 0.86 Composition 4 group 411±25 2.63 0.86 Composition 5 group 426±33 2.73 1.03

[0061] As can be seen from Table 2, compared with the 10% lactic acid damage group, the compositions 1-5 of the present application can restore the dermis layer thickness of the mice. Meanwhile, the combination index of the compositions 2-4 is less than 1, indicating that the recombinant collagen type I and the LPG small molecule peptide have synergistic effect in restoring the dermis layer thickness when the ratio is 250:1-5:1. In particular, the combination index of the compositions 3 and 4 is less than 0.9, indicating that the recombinant collagen type I and the LPG small molecule peptide have significant synergistic effect in restoring the dermis layer thickness when the ratio is 25:1-5:1. In the skin, the recombinant collagen type I mainly plays a role in the form of collagen fibers, which not only endows the tissue with flexibility and tear resistance, but also promotes the growth of epithelial cells and promotes skin repair. On the other hand, no report is found about the function of the LPG small molecule peptide, and the present application first discovers that the LPG small molecule peptide has the functions of increasing the dermis layer thickness of the skin and promoting skin repair. Therefore, the present application also provides the use of the LPG small molecule peptide in the preparation of cosmetics for increasing the dermis layer thickness of the skin and promoting skin repair. The change of the dermis structure is the main internal physiological reason for skin aging. Studies have shown that the dermis thickness becomes thinner after skin aging. The above study of the present application shows that the combination of the recombinant collagen type I and the LPG small molecule peptide can synergistically increase the dermis layer thickness of the skin, thereby playing an anti-wrinkle and firming effect.

[0062] Example 3 Product Efficacy Test One

[0063] As an example of raw materials, a recombinant collagen composition was prepared, which comprises: the recombinant collagen type I, the recombinant collagen type III and the Mini small molecule recombinant collagen peptides APG and LPG, wherein the weight ratio of the recombinant collagen type III to the Mini small molecule recombinant collagen peptide APG is in the range of 10:1-100:1, and the weight ratio of the recombinant collagen type I to the Mini small molecule recombinant collagen peptide LPG is in the range of 10:1-100:1.

[0064] As another example of raw materials, on the basis of the above-prepared recombinant collagen composition, an orchistem extract composition (Orchistem TM , a product of Provital Company) was added, so that the ratio of the sum of the weights of the recombinant collagen type III, the recombinant collagen type I, the APG tripeptide and the LPG tripeptide to the weight of the orchistem extract is 400:1-50:1, thereby preparing a recombinant collagen-orchistem extract composition. The same amount of the above-mentioned recombinant collagen composition raw material, the above-mentioned recombinant collagen-orchistem extract composition raw material and a commercially available collagen composition were respectively used as the only efficacy component, and the same production process was used to make a face cream, which was used in the following product efficacy test.

[0065] The volunteers were applied with the above cream for test, and the specific steps were as follows:

[0066] Forty-three volunteers aged 25-45 years old were recruited, and the initial dermal density and elastic fiber content of the left and right faces were tested after cleansing as the test results of day 0. Three 3x3 cm areas were selected on the left and right faces, respectively. The left face was applied with the above prepared cream containing the commercial collagen composition as the control group, and the right face was applied with the above prepared cream containing the raw material of the recombinant collagen composition or the raw material of the recombinant collagen-extract of Phrymaceae composition as experimental group 1 or experimental group 2, respectively. After two times a day, no other skin care products were used, and the test period lasted for 28 days. The amount used each time was 2 mL.

[0067] The skin ultrasonic diagnostic instrument (model: Ultrascan UC22) produced by Germany CK Company was used to determine the operation according to the instructions of its product manual, and the dermal density improvement rate, skin elastic fiber improvement rate, collagen fiber harmonic intensity improvement rate and aging index improvement rate on the 14th day and the 28th day were calculated based on day 0. The results are shown in Tables 3-6.

[0068]

[0069] Among the above indexes, the higher the dermal density value, the higher the content of collagen and elastic fiber in the dermal layer. The higher the elastic fiber fluorescence intensity value, the better the skin elasticity. The higher the collagen fiber harmonic intensity value, the better the skin condition. The lower the aging index ELCOR value, the younger the skin condition.

[0070] With the increase of age, the collagen and elastic fiber of the skin will gradually be lost, leading to skin relaxation, sagging, no longer tight, and appearance of aging performance. The product of the application can promote the generation of collagen and elastic fiber, increase the density of the dermal layer, make the skin keep tight and more elastic, and thus present a more young state.

[0071] Example 4 Human wrinkle resistance efficacy test

[0072] Test item: wrinkle area (crow's feet, nasolabial folds, fish tail wrinkle)

[0073] Test sample: cream product containing recombinant collagen-extract of Phrymaceae composition, commercial cream product

[0074] The cream product containing recombinant collagen-orchid extract composition for testing includes the following components: by mass percentage, 0.35% of recombinant type III collagen, 0.19% of recombinant type I collagen, 0.07% of anti-wrinkle functional peptide APG, 0.027% of firming functional peptide LPG, 0.0123% of orchid (CALANTHE DISCOLOR) extract, 2.8% of glycerol, 0.25% of sodium hyaluronate, 4% of isononyl isononanoate, 3% of panthenol, 2.8% of octisalate, 2% of squalane, 0.5% of xanthan gum, 0.8% of cetyl stearyl alcohol, 0.42% of hydroxypropyl methyl cellulose, 0.22% of Rhus succedanea fruit wax, 2% of polysorbate-80, the pH value is adjusted to 7 with an aqueous solution of aminomethyl propanol, and the balance is purified water.

[0075] The preparation method of the cream product containing recombinant collagen-orchid extract composition for testing is as follows:

[0076] (1) Accurately weigh glycerol, panthenol, octisalate, sodium hyaluronate, recombinant collagen composition, and xanthan gum, and mix them evenly with part of purified water in a main pot, stir evenly, heat, and keep at 80±2°C for 30 min;

[0077] (2) Accurately weigh cetyl stearyl alcohol, squalane, and hydroxypropyl methyl cellulose, heat and dissolve, and keep at 80-85°C for 10 min;

[0078] (3) After the temperature keeping is completed, the raw materials in (2) are added to the main pot, and stirred and homogenized until the material is uniform, and isononyl isononanoate and polysorbate-80, which are dispersed in advance, are added to the main pot, and emulsified and stirred until the material is uniform;

[0079] (4) Cool to 45-50°C, add the dissolved orchid (CALANTHE DISCOLOR) extract and Rhus succedanea fruit wax to the main pot, stir evenly, and cool to below 30°C to obtain the product.

[0080] Test instrument: Visia Image-Pro Plus

[0081] Test object: Select healthy male and female volunteers aged 25-55 years old, with facial wrinkle crow's feet 1-3 levels, marionette lines 1-3 levels, and fish tail wrinkles 2-5 levels, and the number of people is 35.

[0082] Test method: T / ZHCA006-2019 Cosmetics Anti-wrinkle Effect Test Method.

[0083] Method of use: Take an appropriate amount of the product on the palm, evenly apply it to the face, gently massage until absorbed, and use it in the morning and evening.

[0084] Test standard: The smaller the wrinkle area, the better the improvement of skin wrinkles.

[0085] Environmental conditions: The test environment conditions during the whole test process were controlled as follows: temperature 21±1℃, humidity: 50±10% RH.

[0086]

[0087] Test result explanation:

[0088] The cream product of the present application significantly decreased by 2.6% after 7 days of use compared with before use; the commercially available cream product significantly decreased by 1.5% after 7 days of use compared with before use;

[0089] Measurement: The smaller the wrinkle area, the better the improvement of skin wrinkles.

[0090] The test results of the forehead wrinkle area are shown in Table 4-2:

[0091]

[0092] Test result explanation:

[0093] The cream product of the present application significantly decreased by 2.0% after 7 days of use compared with before use; the commercially available cream product significantly decreased by 1.5% after 7 days of use compared with before use;

[0094] Measurement: The smaller the wrinkle area, the better the improvement of skin wrinkles.

[0095] The test results of the fish tail wrinkle area are shown in Table 4-3:

[0096]

[0097] Test result explanation:

[0098] The cream product of the present application significantly decreased by 2.2% after 7 days of use compared with before use; the commercially available cream product significantly decreased by 1.9% after 7 days of use compared with before use; Measurement: The smaller the wrinkle area, the better the improvement of skin wrinkles.

[0099] Example 5 Human firming efficacy test

[0100] Test item: Skin elasticity (R2, F4) numerical test

[0101] Test sample: Same as Example 4

[0102] Test instrument: Cutometer MPA 580

[0103] Test subjects: 35 healthy male and female volunteers aged 25-55 years old were selected.

[0104] Test method: T / ZHCA 005-2019 cosmetic influence on skin elasticity test method.

[0105] Method of use: Take an appropriate amount of the product on the palm, evenly apply it to the face, gently massage until absorbed, and use it in the morning and evening.

[0106] Test standard: The larger the R2 measurement value, the greater the skin elasticity; the smaller the F4 measurement value, the greater the skin elasticity. Environmental conditions: The test environment conditions during the entire test process are controlled: temperature 21±1℃, humidity: 50±10%RH. ① The skin elasticity R2 test results are shown in Table 5-1:

[0107]

[0108] Test result explanation:

[0109] The cream product of the present application significantly increased by 8.2% after 7 days of use compared with before use of the product; the commercially available cream product significantly increased by 6.3% after 7 days of use compared with before use of the product;

[0110] Measurement: The higher the test value R2, the better the skin elasticity.

[0111] ② The skin elasticity F4 test results are shown in Table 5-2:

[0112]

[0113] Test result explanation:

[0114] The cream product of the present application significantly decreased by 17.6% after 7 days of use compared with before use of the product; the commercially available cream product significantly decreased by 15.8% after 7 days of use compared with before use of the product; Measurement: The lower the test value F4, the better the skin firmness.

Claims

1. A recombinant collagen-Cymbidium goeringii extract composition with anti-wrinkle and firming effects, comprising recombinant type III collagen, recombinant type I collagen, APG tripeptide, LPG tripeptide, and Cymbidium goeringii extract as active ingredients, and a cosmetically acceptable carrier, wherein, In this composition, the weight ratio of recombinant type III collagen to APG tripeptide is 250:1 to 5:1, and / or the weight ratio of recombinant type I collagen to LPG tripeptide is 250:1 to 5:

1. The weight ratio of the sum of the recombinant type III collagen, recombinant type I collagen, APG tripeptide and LPG tripeptide to the weight of the *Symplocos edulis* extract is 700:1 to 35:

1. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID No:1; The amino acid sequence of the recombinant type I collagen is shown in SEQ ID No:

2.

2. The recombinant collagen-shrimp extract composition according to claim 1, wherein, The weight ratio of the recombinant type III collagen to APG tripeptide is 25:1 to 5:1, and / or the weight ratio of the recombinant type I collagen to LPG tripeptide is 25:1 to 5:

1.

3. A cosmetic product comprising the recombinant collagen-shrimp extract composition as described in claim 1 or 2, and one or more selected from solvents, moisturizers, skin conditioning agents, emulsifiers, skin conditioning agents, thickeners, and pH adjusters; Preferably, the solvent is purified water; Preferably, the moisturizer is one or a combination selected from glycerin, 1,2-pentanediol, caprylyl glycol and panthenol; Preferably, the skin conditioning agent is one or more selected from maltodextrin, acetylated sodium hyaluronate, chlorella extract, hydroxypropyl methylcellulose and sunflower seed oil; Preferably, the emulsifier is one or more selected from cetearyl alcohol, sorbitan oleate, arachidonic acid glucoside, and polysorbate-80; Preferably, the emollient is one or more selected from caprylic / capric triglyceride, polydimethylsiloxane, shea butter, squalane, and lacquer wax; Preferably, the thickener is one or more selected from carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, cetearyl alcohol, and Carrageenan extract; Preferably, the pH adjuster is one or more selected from aminomethylpropanol, citric acid, sodium dihydrogen phosphate, and disodium hydrogen phosphate.

4. The cosmetic according to claim 3, wherein it is formulated in a form suitable for application to the skin; for example, it may be an aqueous solution, lotion, cream, mask, etc.

5. The method for preparing the cosmetic according to claim 3, comprising the following steps: (1) Add the moisturizer, thickener, collagen, emollient, emulsifier and purified water into the main container, heat and dissolve at 80℃-85℃, and emulsify until the material is uniform; (2) Start cooling down to 40℃-45℃, add pH adjuster and some skin conditioning agent to the main container and stir well; (3) Under stirring conditions, add the *Cymbidium goeringii* extract and the remaining skin conditioning agent sequentially to the main container and stir until homogeneous; and (4) Cool to below 30°C and stir evenly to obtain the cosmetic containing the recombinant collagen-shrimp extract composition.

6. Use of the recombinant collagen-shrimp extract composition according to claim 1 or 2 in the preparation of a cosmetic or pharmaceutical product for anti-wrinkle and firming purposes, wherein the cosmetic or pharmaceutical product is formulated in a form suitable for application to the skin.

7. The use according to claim 6, wherein, The cosmetics are formulated as liquids, lotions, creams, or masks.

Images