Scalp care composition and application thereof

Abstract

The invention discloses a scalp care composition and application thereof. Effective components of the scalp care composition are agilawood extract and tetrahydromethylpyrimidine carboxylic acid or derivatives thereof. Through reasonable compounding of the agilawood extract and the tetrahydromethylpyrimidine carboxylic acid or the derivative thereof, secretion of insulin-like growth factor-1 (IGF-1) is effectively promoted, so that the number of hair follicles is increased, the growth period of the hair follicles is prolonged, the hair follicle health is promoted and / or maintained, and the scalp health can be promoted and / or maintained; the effects of improving or / and preventing alopecia, growing hair, increasing hair density, strengthening hair roots, improving head blood circulation and the like are achieved.

Description

Technical Field

[0001] This invention relates to a scalp care composition and its application, specifically to a scalp care composition containing agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives, and its application, belonging to the field of cosmetic technology. Background Technology

[0002] The skin contains two special types of fibroblasts: dermal papillae and dermal sheath. These fibroblasts constitute the main signaling niche of hair follicles, interacting with hair follicle cells by secreting growth factors, cytokines, and other signaling molecules. They regulate cell proliferation, differentiation, and functional expression, influencing the cyclical growth and health of hair. Dermal papillae cells receive oxygen and nutrients from the body via blood circulation and then deliver them to hair cells. Therefore, dermal papillae cells are crucial for hair growth, and their number determines the size and shape of the hair.

[0003] The unique feature of hair follicles is that they form follicle embryos at regular intervals (i.e., the hair follicle cycle) after an individual is born and regenerate repeatedly throughout their life. Healthy hair follicles undergo cyclical transitions between rapid growth phases, regression phases, and relatively resting phases. The growth and development of hair is closely related to the cyclical changes of hair follicles.

[0004] Insulin-like growth factor-1 (IGF-1) plays a crucial role in hair growth. Hair follicles contain abundant IGF-1-activated cells, which can improve scalp blood circulation, regulate the proliferation, migration, and differentiation of dermal and keratinocytes in hair follicles, increase the number of hair follicles, prolong the anagen phase, and awaken dormant hair follicles. Furthermore, IGF-1 can inhibit apoptosis of hair follicle stem cells, thus protecting the hair follicles. In recent years, IGF-1 has been reported to enhance the growth of cultured hair follicles (MPPhilpott, et al. Effects of Insulin and Insulin-Like Growth Factors on Cultured Human Hair Follicles: IGF-1 at Physiologic Concentrations Is an Important Regulator of Hair Follicle Growth In Vitro.[J].The Journal of Investigative Dermatology, 1994, 102:857-861.). Local injection of IGF-1 can increase the number of hair follicles and prolong the hair growth phase during the transition from the anagen phase to the telogen phase. Simultaneously, immunological analysis showed that IGF-1 can also stimulate the proliferation of hair follicle cells in the anagen matrix and downregulate TGF-β1 expression in hair follicles. These results indicate that IGF-1 is a broad-spectrum and effective stimulator of mouse hair follicle development in vivo (Jingjie Li, et al. Exogenous IGF-1 promotes hair growth by stimulating cell proliferation and down regulating TGF-β1 in C57BL / 6 mice in vivo.[J]. Growth Hormone & IGF Research 24 (2014) 89–94.). Summary of the Invention

[0005] The purpose of this invention is to provide a scalp care composition comprising agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives. The combination of these two ingredients can effectively promote IGF-1 secretion and exhibits a synergistic effect in promoting IGF-1 secretion, thereby effectively promoting and / or maintaining hair follicle health, and thus promoting and / or maintaining scalp health. Moreover, the ingredients are natural and safe.

[0006] The specific technical solution of this invention is as follows: One aspect of this application relates to compositions, which are any compositions capable of achieving the effects described in this application. The compositions include, but are not limited to, the simultaneous or sequential use of the components. "Simultaneous use" includes using them together in the same formulation or separately in different formulations. "Sequential use" includes using them sequentially in different formulations, with no restriction on the order of sequential use.

[0007] A scalp care composition comprising agarwood extract and tetrahydromethylpyrimidine carboxylic acid or a derivative thereof, wherein the combination of agarwood extract and tetrahydromethylpyrimidine carboxylic acid or a derivative thereof has a good effect on promoting IGF-1 secretion, and can promote and / or maintain scalp health.

[0008] Furthermore, in the above composition, the mass ratio of agarwood extract to tetrahydromethylpyrimidine carboxylic acid or its derivative is 0.1-50:1, for example 0.1:1, 0.2:1, 0.3:1, 0.4:1, 0.5:1, 0.6:1, 0.7:1, 0.8:1, 0.9:1, 1.0:1, 1.1:1, 1.2:1, 1.3:1, 1.4:1, 1.5:1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, etc. 1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 41:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1, preferably 0.1-20:1, more preferably 0.3-20:1.

[0009] Tetrahydromethylpyrimidine carboxylic acid, also known as ectoine, has the corresponding INCI name Ectoine.

[0010] Tetrahydromethylpyrimidine carboxylic acid derivatives include hydroxytetrahydromethylpyrimidine carboxylic acid.

[0011] Furthermore, in the above-mentioned scalp care composition, the agarwood extract is a water-soluble extract of agarwood, which can be prepared at home or purchased through commercial channels.

[0012] Furthermore, in the above-mentioned scalp care composition, the agarwood extract is prepared by steam distillation.

[0013] In a specific embodiment of the present invention, the water-soluble extract of agarwood is obtained by using agarwood as raw material and water as solvent, and extracting the aqueous phase by distillation. The obtained aqueous phase is then purified to obtain the agarwood extract.

[0014] Furthermore, impurity removal can be achieved through operations such as filtration.

[0015] In a specific embodiment of the present invention, the method for obtaining the water-soluble extract of agarwood is as follows: screening agarwood that has formed resin, using water as a solvent, heating and distilling, collecting the distillate, condensing the obtained distillate for water-oil separation, removing impurities from the obtained aqueous phase and filtering to obtain the agarwood extract.

[0016] Furthermore, during distillation, the feeding ratio (mass ratio) of the raw material agarwood and the solvent water is 5:1 to 1:5, for example, it can be 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, preferably 1:1.

[0017] Furthermore, the agarwood can be pre-soaked in water before distillation. The ratio (mass ratio) of the raw agarwood and solvent water during soaking is not limited, for example, it can be 5:1 to 1:5.

[0018] Furthermore, the distillation fraction is a fraction of 100-150℃, for example, it can be 100℃, 110℃, 120℃, 130℃, 140℃, or 150℃.

[0019] In a specific embodiment of the present invention, the method for obtaining the water-soluble extract of agarwood is as follows: screen agarwood that has formed resin, wash it, cut it into thin slices or crush it into small pieces, put it in a container and add purified water with an equal weight of agarwood to soak for 8 hours, then add the soaked agarwood to a distillation kettle, use purified water with an equal weight of agarwood as a solvent, heat and distill, collect the fraction at 110 ℃, condense the obtained fraction to a fractionation tower for water and oil separation, remove impurities from the obtained aqueous phase through a filter screen, filter it, and test the agarwood concentration to ensure it is qualified to obtain the agarwood extract.

[0020] In this invention, agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives are used as active ingredients. In addition to agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives as active ingredients for scalp care, the above-mentioned scalp care composition may also include other active ingredients with different effects, physiologically acceptable media, etc.

[0021] In this invention, the physiologically acceptable medium includes solvents, emollients, thickeners, UV filters, antioxidants, emulsifiers, fillers, colorants, preservatives, fragrances, etc.

[0022] As a solvent, such as an aqueous solvent or an oil-based solvent.

[0023] As emollients, such as squalane, isohexadecane, polydimethylsiloxane, jojoba esters, etc.

[0024] As thickeners, such as xanthan gum, polyacrylic acid compounds, cellulose compounds, bentonite, fatty alcohols, etc.

[0025] As UV filters, they include benzophenone, 4-butylmethoxydibenzoylmethane, octocrylene, ethylhexyl methoxycinnamate, ethylhexyl salicylate, phenylbenzimidazole sulfonic acid, or homosalyl ester.

[0026] As antioxidants, such as tocopherol acetate, p-hydroxyacetophenone, BHT and BHA.

[0027] As emulsifiers, such as anionic emulsifiers, cationic emulsifiers, amphoteric emulsifiers, and nonionic emulsifiers.

[0028] As fillers, such as oxides of silica, mica, kaolin, zinc and titanium from natural or synthetic sources; calcium carbonate, magnesium carbonate, magnesium bicarbonate; zinc stearate, magnesium stearate, lithium stearate, zinc laurate, magnesium myristate, etc.

[0029] As colorants, such as iron oxide, carbon black, titanium dioxide, zinc oxide, carmine, carotene, chlorophyll, etc.

[0030] As preservatives, such as phenoxyethanol, benzyl alcohol, benzoic acid, sorbic acid, imidazolidinyl urea, etc.

[0031] As a fragrance ingredient, it can be used in various ways, such as citronellol and tricyclodecenyl acetate.

[0032] In this invention, the other active ingredients with different effects include active ingredients with moisturizing effects, active ingredients with anti-aging effects, active ingredients with whitening effects, etc.

[0033] Active ingredients that provide moisturizing benefits include mannitol, sodium hyaluronate, lactic acid, glycerin, allantoin, and propylene glycol.

[0034] Active ingredients that have anti-aging effects include retinol and its derivatives, peptides, phytosterols, milk thistle extract, etc.

[0035] Active ingredients that have skin-whitening effects include niacinamide, arbutin, phenylethyl resorcinol, ascorbic acid and its derivatives.

[0036] Furthermore, the present invention also provides a method for preparing the above-mentioned scalp care composition. This method is simple, requiring only the physical mixing of the components. For example, when the scalp care composition contains agarwood extract, tetrahydromethylpyrimidine carboxylic acid or its derivatives, and water as a solvent, these three can be directly mixed and stirred until completely dissolved to obtain the scalp care composition. There are no special requirements regarding the mixing order of the components; they can be mixed in pairs or all three together.

[0037] This invention also provides the application of the above-mentioned scalp care composition in the preparation of scalp care products. This composition can promote the expression of IGF-1 in hair follicles, increase the number of hair follicles, prolong the hair follicle growth phase, awaken dormant hair follicles, and inhibit the apoptosis of hair follicle stem cells, thereby protecting hair follicles, promoting and / or maintaining hair follicle health, and consequently promoting and / or maintaining scalp health, achieving effects such as improving and / or preventing hair loss, promoting hair growth, increasing hair density, strengthening hair roots, and improving scalp blood circulation.

[0038] The present invention also provides a scalp care product, which is made of at least the above-described scalp care composition.

[0039] Furthermore, the content of agarwood extract in the scalp care composition is 0.01-5 wt%, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.10%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 3.0%, 4.0%, and 5.0%.

[0040] Furthermore, the content of tetrahydromethylpyrimidine carboxylic acid or its derivatives in the scalp care composition is 0.01-2 wt%, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.10%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%.

[0041] Furthermore, the scalp care product is a topical skin product.

[0042] Furthermore, the scalp care product is a daily necessity or a medicine, the daily necessity is a hair care product, the hair care product is a shampoo-free and / or leave-on hair care product, or a shampoo-free and / or leave-on hair washing product.

[0043] Furthermore, the daily necessities or medicines can be formulated into various suitable dosage forms, including but not limited to sprays, lotions, liquids, gels, ointments, creams, and lotions.

[0044] The present invention has the following beneficial effects: This invention utilizes a rational combination of agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives to effectively promote IGF-1 secretion, thereby increasing the number of hair follicles, prolonging the hair follicle growth phase, promoting and / or maintaining hair follicle health, and further promoting and / or maintaining scalp health, thus achieving the effects of improving and / or preventing hair loss, promoting hair growth, increasing hair density, strengthening hair roots, and improving scalp blood circulation.

[0045] Furthermore, this invention, through the rational combination of agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives, exhibits a synergistic effect in promoting IGF-1 secretion, thereby further enhancing the nourishing effect on the scalp. Attached Figure Description

[0046] Figure 1 The figures show the experimental results for different samples. Detailed Implementation

[0047] The present invention will be further described below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present invention, and are not intended to limit the present invention.

[0048] Unless otherwise defined, the technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions in this specification shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not restrictive.

[0049] Unless otherwise specified, all materials and reagents used in the following examples and comparative examples are mass percentages and are commercially available. Tetrahydromethylpyrimidine carboxylic acid was obtained from Bloomage Biotechnology Co., Ltd., and the agarwood extract, a water-soluble extract of agarwood prepared by steam distillation, was obtained from Zhenjiang Tanyuan Essential Oil Trading Co., Ltd.

[0050] Example 1 0.156g of agarwood extract and 0.1g of tetrahydromethylpyrimidine carboxylic acid were added to purified water and stirred until completely dissolved. The volume was then adjusted to 100ml with purified water to obtain the scalp care composition. The contents of agarwood extract and tetrahydromethylpyrimidine carboxylic acid are shown in Table 1.

[0051] Examples 2-5 Following the preparation method of Example 1, agarwood extract and tetrahydromethylpyrimidine carboxylic acid were added to purified water, stirred until completely dissolved, and then diluted with purified water to obtain scalp care compositions with different contents of agarwood extract and tetrahydromethylpyrimidine carboxylic acid as shown in Table 1.

[0052] Comparative Examples 1-4 Following the preparation method of Example 1, agarwood extract or tetrahydromethylpyrimidine carboxylic acid was added to purified water, stirred until completely dissolved, and then diluted with purified water to obtain the scalp care composition shown in Table 1.

[0053] Table 1 Test case: Insulin-like growth factor-1 test The cells used in this experiment were 2D immortalized human dermal papilla cells (KNU-201).

[0054] (1) Test reagents (2) Test instruments (3) Test method 1) Cell seeding: Cells in the logarithmic growth phase were cultured in Prigrow III medium containing 10% FBS (fetal bovine serum). When the cell density reached approximately 80%, the cells were digested with 0.05% trypsin, centrifuged at 1000 rpm for 5 min, resuspended and counted, and seeded into 24-well plates. The cells were then cultured in an incubator at 37°C and 5% CO2.

[0055] 2) Sample processing: After 24 hours of incubation, the culture medium was removed, and 1 ml of each of the scalp care compositions from the examples and comparative examples was added. The control group was incubated with 1 ml of Prigrow III medium containing 10% FBS. The plates were then incubated for another 24 hours. After exposure, the supernatant was collected, centrifuged, and IGF-1 secretion was detected using an ELISA kit.

[0056] 3) Testing: Remove the ELISA kit and the sample (cell culture supernatant) and allow them to warm to room temperature for 30 minutes before performing the detection.

[0057] 4) Data Analysis: Calculate the concentration of IGF-1 after sample treatment based on the standard curve; Standard curve: y = 0.0081x + 0.0118 (R²) 2 =0.9997), where y is the IGF-1 concentration and x is the absorbance value.

[0058] Relative IGF-1 secretion level = (IGF-1 concentration in sample group / IGF-1 concentration in control group) * 100% The t-test was used to compare with the control group. *P<0.05 indicates a statistically significant difference, **P<0.01 indicates a statistically significant difference, and ***P<0.001 indicates an extremely statistically significant difference.

[0059] (4) Test results Test results are as follows Figure 1 As shown. Comparative Examples 1-4 showed no significant difference from the control group, indicating that agarwood extract or tetrahydromethylpyrimidine carboxylic acid itself does not promote IGF-1 secretion. However, Examples 1-5, which combined agarwood extract and tetrahydromethylpyrimidine carboxylic acid in a certain proportion, showed a significant difference from the control group, indicating that they could significantly promote IGF-1 secretion, thereby effectively promoting and / or maintaining hair follicle health, and thus promoting and / or maintaining scalp health.

Claims

1. A scalp care composition, characterized in that, Including agarwood extract and tetrahydromethylpyrimidine carboxylic acid or its derivatives.

2. The composition according to claim 1, characterized in that, The mass ratio of agarwood extract to tetrahydromethylpyrimidine carboxylic acid or its derivatives is 0.1-50:

1.

3. The composition according to claim 1, characterized in that, The mass ratio of agarwood extract to tetrahydromethylpyrimidine carboxylic acid or its derivatives is 0.1-20:

1.

4. The composition according to any one of claims 1-3, characterized in that, The agarwood extract is a water-soluble extract of agarwood.

5. The composition according to claim 1, characterized in that, It also includes media that are physiologically acceptable to the human body.

6. Use of the scalp care composition according to any one of claims 1-5 in the preparation of scalp care articles.

7. The application according to claim 6, characterized in that, The composition promotes the expression of IGF-1 in hair follicles.

8. A scalp care product, characterized in that, It is made from at least the scalp care composition according to any one of claims 1-5.

9. The article of claim 8, characterized in that, The content of agarwood extract in the product is 0.01-5 wt%, and the content of tetrahydromethylpyrimidine carboxylic acid or its derivatives in the product is 0.01-2 wt%.

10. The article of claim 9, characterized in that, The product in question is a topical skin product.

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