Stable compositions of dihydroartemisinin

Abstract

The present invention provides a stable composition comprising, based on the total weight of the composition: 0.01-1 wt% of dihydroartemisinin; 0.1-30% by weight of fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof; and an aqueous carrier acceptable in the personal care field. The invention also relates to a personal care product comprising said composition.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of natural pharmaceutical chemistry and the field of cosmetics, in particular to a stable composition formed by coating dihydroartemisinin with fatty alcohol polyoxyethylene ether carboxylic acid or fatty alcohol polyoxyethylene ether carboxylic acid salt. BACKGROUND

[0002] In today's fast-paced era, people are always hoping that anything can be quickly, accurately and effectively solved under the background of work and life pressure, and they do not want to invest a lot of time and effort to harvest the desired effect or other negative effects. In this spirit of pursuit, consumers' demand for functional personal care products is becoming more and more vigorous. Gradually, this demand has extended from stay-on products such as color cosmetics and skin care products to rinse-off products such as personal cleansing and care products. Therefore, cosmetics that take into account both efficacy and safety are increasingly favored by consumers. Among the many functional raw materials, natural sources of functional raw materials have more advantages than a variety of traditional non-natural sources of functional active substances.

[0003] Dihydroartemisinin (DHA) is an extremely important derivative of artemisinin, which has higher bioavailability and easier metabolism than artemisinin, greatly improving its pharmaceutical value. According to research in the medical field, DHA has good effects in terms of anti-malaria, anti-tumor, anti-virus, anti-inflammatory, anti-other parasites, anti-fibrosis, blood pressure reduction, and immune regulation. Existing research has found that dihydroartemisinin not only has the above medical effects, but also has a good inhibitory effect on Malassezia, and can be used as a safe and efficient anti-dandruff ingredient to replace traditional anti-dandruff agents.

[0004] Chinese patent CN109939105A discloses the use of artemisinin and its derivatives in the preparation of drugs and cosmetics for improving the barrier function of the stratum corneum and preventing and treating skin inflammation.

[0005] Chinese patent CN116019734A discloses the anti-dandruff application of dihydroartemisinin. The patent research found that dihydroartemisinin has a clear inhibition zone at a concentration of 5mM and 10mM, and the inhibition zone of 10mM is greater than the positive control OCT, indicating that dihydroartemisinin has a good inhibitory effect on Malassezia.

[0006] However, DHA is poorly soluble in water and other aqueous media, and contains a special peroxide group in the molecule, which is not stable to heat, and is easily decomposed by moisture, heat and reducing substances. In order to improve the water solubility and stability of DHA and reduce its toxicity, the common method in the medical field at present is to encapsulate DHA into drug carriers. For example, Chiara Righeschi et al. prepared two kinds of DHA-loaded liposomes using egg yolk lecithin and cholesterol, which can significantly improve the water solubility of DHA and reduce its toxicity to normal cells. Chen Fangwei et al. used hydroxypropyl-β-cyclodextrin (HP-β-CD) as a carrier to encapsulate DHA, and the feasibility of encapsulation was revealed by molecular simulation. The results showed that the encapsulation of DHA by HP-β-CD was not only feasible but also could significantly improve the water solubility of DHA. The above cases are all from the field of pharmacy, and there is no new report in the field of daily chemical industry. It is of great significance to expand the application field of DHA if it can be applied to cosmetic formulations and stably exert its effect.

[0007] It is unexpectedly found in the present application that the fatty alcohol polyoxyethylene ether carboxylic acid or fatty alcohol polyoxyethylene ether carboxylic acid salt can solubilize DHA, thereby greatly improving the solubility of DHA in aqueous system, and the combination of the two has a stable appearance under high and low temperature and normal temperature conditions. Therefore, the present application provides a stable composition formed by wrapping dihydroartemisinin with fatty alcohol polyoxyethylene ether carboxylic acid or fatty alcohol polyoxyethylene ether carboxylic acid salt. SUMMARY

[0008] In one aspect, the present application provides a stable composition, which comprises, based on the total weight of the composition:

[0009] 0.01-1% by weight of dihydroartemisinin;

[0010] 0.1-30% by weight of fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof; and

[0011] an aqueous carrier acceptable in the field of personal care.

[0012] In a preferred embodiment, the composition of the present application comprises 0.1-0.5% by weight of dihydroartemisinin.

[0013] In a preferred embodiment, the composition of the present application comprises 1-20% by weight of fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof, more preferably 15% by weight of fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof.

[0014] In a preferred embodiment, the fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof is C 12-22 The fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof, more preferably, the fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof is lauryl polyether-5 carboxylic acid or salt thereof.

[0015] In preferred embodiments, the composition of the present application has a water content greater than 50% by weight.

[0016] In another aspect, the present application also relates to a personal care product comprising the composition of the present application. In preferred embodiments, the personal care product is a shampoo, a body wash, or a facial cleanser. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 Stability photos of 4-week samples of Examples 1-5 are shown.

[0018] Figure 2 Stability photos of 4-week samples of Examples 7-11 are shown.

[0019] Figure 3 Stability comparison photos of 4-week samples of Example 6, Comparative Example 1, and Comparative Example 2 are shown.

[0020] Figure 4 Stability comparison photos of 4-week samples of Example 12 and Comparative Example 3 are shown. DETAILED DESCRIPTION

[0021] The present application provides a stable composition. The composition is formed by a combination of fatty alcohol polyoxyethylene ether carboxylic acid / fatty alcohol polyoxyethylene ether carboxylic acid salt and dihydroartemisinin (DHA), and the composition has a stable appearance and can be used in efficacy-type daily-use products. The fatty alcohol polyoxyethylene ether carboxylic acid and DHA can form a stable white gel-like liquid, and the fatty alcohol polyoxyethylene ether carboxylic acid salt and DHA can form a stable colorless transparent liquid. When the aqueous solution of the fatty alcohol polyoxyethylene ether carboxylic acid is adjusted to a pH greater than 7 with a sodium hydroxide solution, the solution is colorless and transparent.

[0022] When the vesicles or micelles formed by the fatty alcohol polyoxyethylene ether carboxylic acid and the fatty alcohol polyoxyethylene ether carboxylic acid salt in water can encapsulate hydrophobic ingredients, they have a solubilizing effect. At the same time, during cleaning dilution or use, the vesicles or micelles open, and the solubilized ingredients can be slowly released, so they also have a sustained-release effect on the active ingredients.

[0023] The present application takes advantage of the encapsulation, solubilization, and sustained-release effects of the micelles formed by the fatty alcohol polyoxyethylene ether carboxylic acid / fatty alcohol polyoxyethylene ether carboxylic acid salt, not only increasing the water solubility of DHA, but also improving its stability and reducing its cytotoxicity. Therefore, the composition of the present application greatly expands the application scenarios of DHA.

[0024] For the sake of providing a clearer description, some quantitative expressions given herein are not modified with the term "about". It should be understood that whether or not the term "about" is expressly used, every quantity given herein is intended to refer to the actual given value, and also to be approximated by a reasonable amount that one of ordinary skill in the art can deem to be the

[0025] For the sake of providing a clearer description, some quantitative expressions given herein are recited as a range from about X amount to about Y amount. It should be understood that when a range is recited, the range is not limited to the recited upper and lower limits, but includes the entire range of quantities between about X amount and about Y amount or any amount therebetween.

[0026] Dihydroartemisinin

[0027] Dihydroartemisinin (DHA) is one of the most important derivatives of artemisinin, and has the characteristics of higher bioavailability and easier metabolism compared to artemisinin. According to research in the medical field, DHA has good effects in terms of anti-malaria, anti-tumor, anti-virus, anti-inflammatory, anti-other parasites, anti-fibrosis, antihypertensive, and immune regulation. Existing research has found that dihydroartemisinin not only has the above medical effects, but also has a good inhibitory effect on malassezia, and can be used as a safe and efficient anti-dandruff ingredient of natural origin to replace traditional anti-dandruff agents.

[0028] In some embodiments, the composition of the present application comprises 0.01-1% by weight of dihydroartemisinin. In preferred embodiments, the composition of the present application comprises 0.01-0.6% by weight of dihydroartemisinin. In more preferred embodiments, the composition of the present application comprises 0.1-0.5% by weight of dihydroartemisinin.

[0029] Fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof

[0030] Fatty alcohol polyoxyethylene ether carboxylate (AEC) is a high performance surfactant, which is modified from non-ionic surfactant and has the characteristics of both anionic and non-ionic surfactants. Because of the oxygen ethylene bond in the molecule, it has the properties of non-ionic surfactant, and its ionic characteristics can be changed by adjusting the pH. AEC is resistant to hard water and has good compatibility with cationic surfactants. At the same time, because the terminal hydrophilic group is an acid group, it is mild to the human body. Therefore, fatty alcohol polyoxyethylene ether carboxylate has good water solubility, chemical stability, wetting property, emulsifying property, hard water resistance, and good foam performance, strong washing power and calcium soap dispersing power, and low skin irritation, and can be used as a cosmetic, a washing product, a crude oil demulsifier, a thick oil viscosity reducer, a sewage treatment agent, a softener, an emulsifier, an antistatic agent, etc. Han Jinying studied the aggregation behavior of fatty alcohol polyoxyethylene ether carboxylate (AEC) acid type product in aqueous solution by transmission electron microscopy and dynamic light scattering method, and found that it can form vesicles due to hydrogen bonding in aqueous solution, thus showing a light blue to milky white turbidity state.

[0031] When the aqueous solution of fatty alcohol polyoxyethylene ether carboxylic acid is adjusted to pH greater than 7 with sodium hydroxide solution, the solution is colorless and transparent.

[0032] When the vesicles or micelles formed by fatty alcohol polyoxyethylene ether carboxylic acid and fatty alcohol polyoxyethylene ether carboxylate in water can wrap hydrophobic ingredients, they have a solubilizing effect. At the same time, during the process of cleaning dilution or use, the vesicles or micelles open, and the solubilized ingredients can be slowly released, so they also have a slow-release effect on active ingredients.

[0033] The present application finds that fatty alcohol polyoxyethylene ether carboxylic acid and fatty alcohol polyoxyethylene ether carboxylate can solubilize DHA, thereby greatly improving its solubility in aqueous systems. The combination of the two has a stable appearance under high and low temperature and room temperature conditions, indicating that DHA is wrapped by the micelles of fatty alcohol polyoxyethylene ether carboxylic acid and fatty alcohol polyoxyethylene ether carboxylate, and the stability is improved. The composition has a good inhibitory effect on malassezia, and can replace traditional anti-dandruff agents and be applied to anti-dandruff products.

[0034] In some embodiments, the fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof used in the composition of the present application is C 12-22 The fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof used in the composition of the present application is C 12-20 The fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof used in the composition of the present application is C 12-18 The fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof used in the composition of the present application is C 12-16fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof. In a specific embodiment, the fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof employed in the compositions of the present application is laureth-5 carboxylic acid or a salt thereof. In a specific embodiment, the present application employs laureth-5 carboxylic acid available under the trade name CED5 from Innospec (Beijing) Co. Ltd. CED5 laureth-5 carboxylic acid available from Innospec (Beijing) Co. Ltd.

[0035] In some embodiments, sodium hydroxide is employed to neutralize the fatty alcohol polyoxyethylene ether carboxylic acid to form a sodium salt of the fatty alcohol polyoxyethylene ether carboxylic acid.

[0036] In some embodiments, the compositions of the present application comprise 0.1-30 wt.% of the fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof. In a preferred embodiment, the compositions of the present application comprise 1-20 wt.% of the fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof. In a specific embodiment, the compositions of the present application comprise 15 wt.% of the fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof.

[0037] Stable composition

[0038] The present application provides a multifunctional stabilizing composition that greatly improves the solubility and stability of DHA in aqueous systems, which can be applied in efficacy daily-use products. The present technology can greatly expand the application of the active ingredient of natural origin-dihydroartemisinin in other fields.

[0039] In addition to the above components, the compositions of the present application also contain a balance of water. Thus, the compositions of the present application can also comprise water or other aqueous carriers.

[0040] In a preferred embodiment, the compositions of the present application have a water content of at least 50 wt.%. For example, the compositions of the present application have a water content of greater than 50 wt.%, greater than 55 wt.%, greater than 60 wt.%, greater than 65 wt.%, greater than 70 wt.%, greater than 75 wt.%, or greater than 80 wt.%. In some embodiments, the compositions of the present application have a water content of 50-95 wt.%, preferably 60-90 wt.%, 60-88 wt.%, 65-88 wt.%, 70-88 wt.%, or 75-88 wt.%.

[0041] Personal care product

[0042] The present application also relates to personal care products, which can comprise the compositions of the present application. In some specific embodiments, such personal care products can be in the form of facial cleansers, body washes, shampoos, etc. In a preferred embodiment, the personal care product is a shampoo product.

[0043] The product can be applied to the skin or hair of a user in any desired manner. In some aspects, the product can be applied directly by hand, or a device such as a towel, sponge, or other apparatus can be used to apply the product. The composition is advantageously applied to wetted hair or skin to facilitate application. The composition can be left on the area of application for a desired level of time, such as from about 5 seconds to about 5 minutes, and then rinsed off with water to remove from the area of application.

[0044] EMBODIMENT

[0045] The present application is further described in connection with the following specific examples. It is to be understood that the examples are merely illustrative of specific types of embodiments of the application and do not limit the scope of the application. One skilled in the art can effect non-essential changes and modifications of the application in view of these examples without departing from the scope of the application. Unless otherwise noted, the test methods used in the following examples were conducted under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise indicated, all percentages and parts are by weight.

[0046] The experimental materials used in the embodiments of the present application are as follows:

[0047] Dihydroartemisinin, purity > 90%, provided by Institute of Chinese Medicine, Chinese Academy of Chinese Medical Sciences;

[0048] Lauryl alcohol polyether-5 carboxylic acid, trade name CED5, active content 100%, purchased from Innochem (Beijing) Co., Ltd;

[0049] Sodium hydroxide, trade name Sodium Hydroxide, purity ≥ 98%, purchased from Tianjin Bocai Chemical Co., Ltd;

[0050] Sodium lauroamphoacetate, trade name MIRANOL ULTRA L-32, active content 30%, purchased from Solvay Investment Co., Ltd;

[0051] Sodium cocamphoacetate, trade name MIRANOL ULTRA C-32, active content 30%, purchased from Solvay Investment Co., Ltd;

[0052] Disodium lauryl alcohol polyether sulfosuccinate, trade name Ucefactant MES L30, active content 30%, purchased from Guangzhou Xingye Science and Technology Co., Ltd.

[0053] Example 1: Preparation of a stable composition of dihydroartemisinin coated with fatty alcohol polyoxyethylene ether carboxylic acid

[0054] Take 15 parts by mass of lauryl alcohol polyether-5 carboxylic acid, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until evenly dispersed; take 0.01 parts by mass of dihydroartemisinin and place it in the beaker, mix and stir at 200 rpm until dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0055] Example 2: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylic acid

[0056] Take 15 parts by mass of lauryl alcohol polyether-5 carboxylic acid, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until evenly dispersed; take 0.3 parts by mass of dihydroartemisinin and place it in the beaker, mix and stir at 200 rpm until dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0057] Example 3: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylic acid

[0058] Take 15 parts by mass of lauryl alcohol polyether-5 carboxylic acid, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until evenly dispersed; take 0.4 parts by mass of dihydroartemisinin and place it in the beaker, mix and stir at 200 rpm until dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0059] Example 4: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylic acid

[0060] Take 15 parts by mass of lauryl alcohol polyether-5 carboxylic acid, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until evenly dispersed; take 0.9 parts by mass of dihydroartemisinin and place it in the beaker, mix and stir at 200 rpm until dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0061] Example 5: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylic acid

[0062] Take 15 parts by mass of lauryl alcohol polyether-5 carboxylic acid, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until evenly dispersed; take 1 part by mass of dihydroartemisinin and place it in a beaker, mix and stir at 200 rpm until dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0063] Example 6: Preparation of a stable composition of dihydroartemisinin coated with fatty alcohol polyoxyethylene ether carboxylic acid

[0064] Take 15 parts by mass of lauryl alcohol polyether-5 carboxylic acid, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until evenly dispersed; take 0.5 parts by mass of dihydroartemisinin and place it in a beaker, mix and stir at 200 rpm until dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0065] Test Example 1: Stability test

[0066] Test the appearance of Examples 1-6, and investigate their stability.

[0067] The stability evaluation method is as follows: place the compositions prepared in Examples 1-5 in constant temperature ovens at high temperature (40°C), low temperature (4°C), and room temperature (25°C), and investigate for 30 days. Observe the samples every week for the presence of solid particles, jellification, layering, color change, etc. If the sample shows no change before and after a certain time within 30 days, it is considered to have "passed"; the presence of solid particles is considered to have "precipitated"; the presence of a sticky, viscoelastic gel that is not easy to flow is considered to have "jellified"; the presence of two layers of liquid is considered to have "layered"; and the presence of a color change is considered to have "changed color". The results are shown in the following table.

[0068] Table 1: Stability investigation of different prepared compositions

[0069]

[0070] As can be seen from Table 1, when the amount of dihydroartemisinin added is between 0.01 and 1 parts, it can all be completely dissolved in an aqueous solution containing 15 parts of lauryl alcohol polyether-5 carboxylic acid, and the formed composition can maintain a stable white liquid appearance at high temperature (40°C), low temperature (4°C), and room temperature (25°C) for 1-4 weeks.

[0071] Example 7: Preparation of a stable composition of dihydroartemisinin coated with fatty alcohol polyoxyethylene ether carboxylic acid salt

[0072] Take 0.5 parts by mass of sodium hydroxide, add to a beaker containing 50 parts by mass of deionized water, mix and stir at a speed of 200 rpm at room temperature until completely dissolved; take 15 parts by mass of lauryl alcohol polyoxyethylene-5 carboxylic acid into a beaker, fully stir at 200 rpm until the solution is clear and transparent; take 0.01 parts by mass of dihydroartemisinin into the beaker, fully stir and mix at 200 rpm until the dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0073] Example 8: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylate

[0074] Take 0.5 parts by mass of sodium hydroxide, add to a beaker containing 50 parts by mass of deionized water, mix and stir at a speed of 200 rpm at room temperature until completely dissolved; take 15 parts by mass of lauryl alcohol polyoxyethylene-5 carboxylic acid into a beaker, fully stir at 200 rpm until the solution is clear and transparent; take 0.3 parts by mass of dihydroartemisinin into the beaker, fully stir and mix at 200 rpm until the dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0075] Example 9: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylate

[0076] Take 0.5 parts by mass of sodium hydroxide, add to a beaker containing 50 parts by mass of deionized water, mix and stir at a speed of 200 rpm at room temperature until completely dissolved; take 15 parts by mass of lauryl alcohol polyoxyethylene-5 carboxylic acid into a beaker, fully stir at 200 rpm until the solution is clear and transparent; take 0.4 parts by mass of dihydroartemisinin into the beaker, fully stir and mix at 200 rpm until the dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0077] Example 10: Preparation of a stable composition of dihydroartemisinin wrapped by fatty alcohol polyoxyethylene ether carboxylate

[0078] Take 0.5 parts by mass of sodium hydroxide, add to a beaker containing 50 parts by mass of deionized water, mix and stir at a speed of 200 rpm at room temperature until completely dissolved; take 15 parts by mass of lauryl alcohol polyoxyethylene-5 carboxylic acid into a beaker, fully stir at 200 rpm until the solution is clear and transparent; take 0.9 parts by mass of dihydroartemisinin into the beaker, fully stir and mix at 200 rpm until the dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0079] Example 11: Preparation of stable composition of dihydroartemisinin coated with fatty alcohol polyoxyethylene ether carboxylate

[0080] Take 0.5 parts by mass of sodium hydroxide, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until completely dissolved; take 15 parts by mass of lauryl polyether-5 carboxylic acid and place it in a beaker, fully stir at 200 rpm until the solution is clear and transparent; take 1 part by mass of dihydroartemisinin and place it in a beaker, fully stir and mix at 200 rpm until the dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0081] Example 12: Preparation of stable composition of dihydroartemisinin coated with fatty alcohol polyoxyethylene ether carboxylate

[0082] Take 0.5 parts by mass of sodium hydroxide, add to a beaker containing 50 parts by mass of deionized water, mix and stir at room temperature at a speed of 200 rpm until completely dissolved; take 15 parts by mass of lauryl polyether-5 carboxylic acid and place it in a beaker, fully stir at 200 rpm until the solution is clear and transparent; take 0.5 parts by mass of dihydroartemisinin and place it in a beaker, fully stir and mix at 200 rpm until the dihydroartemisinin is completely dissolved, then make up to 100 parts with deionized water, continue to stir at a stirring speed of 200 rpm for 10 minutes until completely dispersed and uniform.

[0083] Test Example 2: Stability test

[0084] The appearance of test examples 7-12 was observed for stability.

[0085] The stability evaluation method is as follows: the transparent composition prepared in examples 7-12 was placed in a constant temperature oven at high temperature (40°C), low temperature (4°C) and room temperature (25°C) for 30 days. The samples were observed every week for the presence of solid particles, jellification, layering, color change, etc. If the sample showed no change before and after a certain time within 30 days, it was considered to have "passed"; the presence of solid particles was considered to have "precipitated"; the presence of a viscous gel that was not easy to flow was considered to have "jellified"; the presence of two layers of liquid was considered to have "layered"; and the presence of color change was considered to have "changed color". The results are shown in the following table.

[0086] Table 2: Stability of different prepared compositions

[0087]

[0088]

[0089] As can be seen from Table 2, when the amount of dihydroartemisinin added is between 0.01 and 1 parts, it can be completely dissolved in the aqueous solution containing 15 parts of sodium lauryl polyether-5 carboxylate to form a colorless transparent non-viscous aqueous type liquid, and the combination of the two can maintain stable colorless transparent appearance for 1 to 4 weeks under high and low temperature and normal temperature conditions.

[0090] Preparation of sodium lauroamphoacetate and dihydroartemisinin combination

[0091] 50 parts by mass of sodium lauroamphoacetate was placed in a beaker, 0.5 parts by mass of dihydroartemisinin was slowly added under stirring at a speed of 200 rpm, and after fully stirring and mixing until dihydroartemisinin was completely dissolved, deionized water was added to make up to 100 parts, and stirring was continued at a speed of 200 rpm for 10 minutes until complete dispersion.

[0092] Preparation of sodium lauroamphoacetate and dihydroartemisinin combination

[0093] 50 parts by mass of sodium lauroamphoacetate was placed in a beaker, 0.5 parts by mass of dihydroartemisinin was slowly added under stirring at a speed of 200 rpm, and after fully stirring and mixing until dihydroartemisinin was completely dissolved, deionized water was added to make up to 100 parts, and stirring was continued at a speed of 200 rpm for 10 minutes until complete dispersion.

[0094] Preparation of sodium lauroamphoacetate and dihydroartemisinin combination

[0095] 50 parts by mass of sodium lauroamphoacetate was placed in a beaker, 0.5 parts by mass of dihydroartemisinin was slowly added under stirring at a speed of 200 rpm, and after fully stirring and mixing until dihydroartemisinin was completely dissolved, deionized water was added to make up to 100 parts, and stirring was continued at a speed of 200 rpm for 10 minutes until complete dispersion.

[0096] Test Example 3: Stability test

[0097] The appearance of Test Comparative Examples 1-3 was tested for stability.

[0098] The stability evaluation method is as follows: the transparent combination prepared in Test Comparative Examples 1-3 was placed in a constant temperature oven at high temperature (40°C), low temperature (4°C) and normal temperature (25°C), and was observed for 30 days to observe whether solid particles precipitated, whether it became a jelly, whether it was layered, whether it changed color, etc. If the sample showed no change before and after a certain time within 30 days, it was considered to have "passed"; if solid particles appeared, it was considered to have "precipitated"; if a viscous gel that was not easy to flow appeared, it was considered to have "jellied"; if the upper and lower layers of the liquid appeared, it was considered to have "layered"; and if the color changed, it was considered to have "changed color". The results are shown in the following table.

[0099] Table 3: Stability investigation of compositions prepared in Comparative Examples 1-3

[0100]

[0101] From the results of Comparative Example 1, Comparative Example 2 and Example 6, it can be seen that the effective content of surfactant is 15%, and the content of dihydroartemisinin is 0.5%, the composition of sodium lauroamphoacetate, sodium cocamphoacetate and dihydroartemisinin in Comparative Example 1 appears color change at room temperature and high temperature, and the appearance is unstable, while the lauryl alcohol polyether-5 carboxylic acid in Example 6 can form a stable white liquid with dihydroartemisinin, which shows that the vesicular structure formed by lauryl alcohol polyether-5 carboxylic acid in aqueous solution can wrap dihydroartemisinin, thereby increasing its stability. From the results of Comparative Example 3 and Example 12, it can be seen that the effective content of surfactant is 15%, and the content of dihydroartemisinin is 0.5%, the composition of lauryl alcohol polyether sulfosuccinate disodium and dihydroartemisinin in Comparative Example 3 appears color change at room temperature and high temperature, and the appearance is unstable, while the sodium lauryl alcohol polyether-5 carboxylate obtained by neutralizing lauryl alcohol polyether-5 carboxylic acid with sodium hydroxide in Example 12 can form a stable colorless transparent liquid with dihydroartemisinin, which shows that the micelles formed by lauryl alcohol polyether-5 carboxylate in aqueous solution can wrap dihydroartemisinin, thereby increasing its stability.

Claims

1. A stable composition comprising, based on the total weight of the composition: 0.01-1 wt.% of dihydroartemisinin; 0.1-30 wt.% of a fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof; and an aqueous carrier acceptable in the personal care field.

2. The composition of claim 1, wherein The composition comprises 0.1-0.5 wt.% of dihydroartemisinin.

3. The composition of claim 1, wherein The composition comprises 1-20 wt.% of a fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof.

4. The composition of claim 3, wherein The composition comprises 15 wt.% of a fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof.

5. The composition of claim 1, wherein The fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof is C 12-22 The fatty alcohol polyoxyethylene ether carboxylic acid or salt thereof is C 6. The composition of claim 6, wherein The fatty alcohol polyoxyethylene ether carboxylic acid or a salt thereof is laureth-5 carboxylic acid or a salt thereof.

7. The composition of claim 1, wherein The composition has a water content greater than 50 wt.%.

8. A personal care product comprising the composition of any one of claims 1-7.

9. The personal care product of claim 8, which is a shampoo product, a body wash, or a facial cleanser.

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