Fermented product of lavender flowers and camellia seed oil, and preparation method and application thereof

Abstract

The present application relates to the field of biological medicine, and discloses a fermentation product of lavender flowers and camellia seed oil, and a preparation method and application thereof. The preparation method of the fermentation product of the lavender flowers and the camellia seed oil comprises the following steps: fermenting a flower oil mixture by using a bumblebee s. sternai yeast, and taking an oil phase product after fermentation; the flower oil mixture is a mixture of the lavender flowers and the camellia seed oil. The method can efficiently extract essential oil in the lavender flowers, the mixed product of the lavender flowers and the camellia seed oil after fermentation has a synergistic effect in efficacy, has good anti-inflammatory and moisturizing effects, and can be applied in anti-inflammatory products and moisturizing products.

Description

Technical Field

[0001] This invention relates to the field of biomedicine, and more specifically, to fermentation products of lavender flowers and camellia seed oil, their preparation methods, and applications. Background Technology

[0002] Lavender, rich in aromatic essential oils, possesses significant ornamental and practical value. Currently, the main methods for utilizing lavender resources are organic solvent extraction, steam distillation, or supercritical carbon dioxide extraction to extract lavender essential oil from lavender flowers. This oil is widely used as a fragrance ingredient in cosmetics, detergents, and food. According to some reports, lavender essential oil has antibacterial, anti-inflammatory, antioxidant, sebum-inhibiting, tyrosinase-inhibiting, collagen-synthesizing, and hair-growth-promoting effects. However, in currently popular extraction processes, organic solvent extraction requires volatile organic solvents such as hexane, posing risks such as solvent residue and environmental pollution; steam distillation requires high energy consumption and generates large amounts of distillation wastewater; and supercritical carbon dioxide extraction requires significant equipment investment, resulting in high production costs. Finding a green and efficient method for extracting lavender essential oil from lavender flowers remains a challenging task.

[0003] In view of this, the present invention is proposed. Summary of the Invention

[0004] The purpose of this invention is to provide fermentation products of lavender flowers and camellia seed oil, their preparation methods and applications, in order to improve at least one of the problems mentioned in the background art.

[0005] This invention is implemented as follows:

[0006] In a first aspect, the present invention provides a method for preparing a fermentation product of lavender flowers and camellia seed oil, comprising:

[0007] The flower oil mixture was fermented using bumblebee Saccharomyces cerevisiae, and the oil phase product was collected after fermentation; the flower oil mixture was a mixture of lavender flower oil and camellia seed oil.

[0008] In an optional embodiment, the lavender flowers are dried lavender flowers, and the mass ratio of dried lavender flowers to camellia seed oil is 1:5~20.

[0009] In an optional implementation, *Bombyx mori* is classified and named as: *Bombyx mori* RTNY120013 Starmerella bombicola RTNY120013 is deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, China. The deposit date is August 12, 2025, and the accession number is CCTCC NO:M 20251820.

[0010] In an optional implementation, the fermentation method includes:

[0011] OD 600nm The bumblebee *Saccharomyces cerevisiae* fermentation broth (≥50%) was mixed with a flower oil mixture and fermented for 40-56 hours. The volume ratio of the bumblebee *Saccharomyces cerevisiae* fermentation broth to the flower oil mixture was 1-5:1-5.

[0012] In an optional implementation, at least one of the following features (1) and (2) is included:

[0013] (1) OD 600nm Before mixing and fermenting ≥50g of bumblebee Saccharomyces cerevisiae ferment broth with the flower oil mixture, the following steps are also included:

[0014] Bumblebee *Saccharomyces cerevisiae* seed culture was cultured in sterilized YPD medium until OD2000. 600nm ≥10;

[0015] The yeast seed culture was inoculated into sterilized YPD medium and cultured until OD... 600nm ≥50 yields Bumblebee Saccharomyces fermentation broth;

[0016] (2) The volume ratio of bumblebee yeast fermentation liquid to flower oil mixture is 1~4:1~2 or 1:2.

[0017] In an optional implementation, at least one of the following features (1) and (2) is included:

[0018] (1) The lavender flowers in the flower oil mixture are dried lavender flower powder that has passed through a 100-mesh sieve;

[0019] (2) The lavender flowers are dried lavender flowers, and the mass ratio of dried lavender flowers to camellia seed oil is 1:8~15, or 1:10~12, or 1:10.

[0020] Secondly, the present invention provides a fermentation product of lavender flowers and camellia seed oil, which is prepared by any of the preparation methods described in the foregoing embodiments.

[0021] Thirdly, the present invention provides a moisturizing product comprising the fermented products of lavender flower and camellia seed oil as described in the foregoing embodiments.

[0022] In an optional embodiment, the content of fermentation product is 0.1~3%wt.

[0023] Fourthly, the present invention provides an anti-inflammatory product comprising the fermentation products of lavender flowers and camellia seed oil as described in the foregoing embodiments.

[0024] The present invention has the following beneficial effects:

[0025] In this invention, *Bumblebee Saccharomyces cerevisiae* produces various hydrolytic enzymes that effectively degrade the plant fibers and cell walls of lavender flowers. Simultaneously, it also hydrolyzes the oil (camellia seed oil), increasing its affinity for the plant tissue (lavender flowers). The combination of these two effects promotes the permeability of the oil (camellia seed oil) to the plant cells (lavender flowers), significantly increasing the release rate of lavender essential oil. This invention combines aqueous-phase plant fermentation technology with oil-phase plant extraction technology to obtain a highly efficient method for obtaining a fermented composition of lavender flowers and camellia seed oil. Experiments have verified that the mixed fermentation of lavender flowers and camellia seed oil has a synergistic effect, and the product exhibits good anti-inflammatory and moisturizing effects, making it highly suitable for use in anti-inflammatory or moisturizing products. Attached Figure Description

[0026] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 Comparison of cytotoxicity against RAW264.7 cells in Examples 1-6;

[0028] Figure 2 Comparison of cytotoxicity of Example 1 and Comparative Examples 1-3 on RAW264.7 cells;

[0029] Figure 3 Examples 1-6 compare the ability of immune cells to inhibit NO expression;

[0030] Figure 4 Comparison of the ability of Example 1 and Comparative Examples 1-3 to inhibit NO expression in immune cells;

[0031] Figure 5 Cytotoxicity of HaCaT cells in Examples 1 and Comparative Examples 1-3;

[0032] Figure 6 Comparison of the ability of Example 1 and Comparative Examples 1-3 to promote AQP-3 expression in HaCaT cells;

[0033] Figure 7 The sample's ability to increase the moisture content of the stratum corneum of the skin;

[0034] Figure 8 The sample's ability to reduce skin moisture loss. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0036] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0037] Based on the problems existing in the current technology, the following considerations were made:

[0038] Oils and fats are esters formed from fatty acids and glycerol. They are excellent solvents for natural plant essential oils and are also an important basic ingredient in cosmetics. Using oils and fats, especially plant-derived oils, to extract fragrance plants allows for the organic combination of natural plant essential oils and natural oils, avoiding organic solvent residues, reducing industrial wastewater, and simplifying extraction equipment and processes. However, plant tissues and cell walls are composed of hydrophilic materials such as cellulose, lignin, and pectin, which are incompatible with oils. This results in poor permeability of oils to plant cells and low extraction efficiency of intracellular plant essential oils. Therefore, improving the extraction efficiency of plant essential oils from oils is a key challenge in the essential oil extraction process.

[0039] In view of the above considerations, the present invention is proposed:

[0040] This invention provides a method for preparing fermentation products of lavender flowers and camellia seed oil, comprising:

[0041] The flower oil mixture was fermented using bumblebee Saccharomyces cerevisiae, and the oil phase product was collected after fermentation; the flower oil mixture was a mixture of lavender flower oil and camellia seed oil.

[0042] Plant fermentation technology is a method to efficiently extract plant essential oils from oils. In this invention, *Bumblebee Saccharomyces cerevisiae* produces various hydrolytic enzymes that effectively degrade the plant fibers and cell walls of lavender flowers. Simultaneously, it also has a certain hydrolytic effect on the oil (camellia seed oil), increasing the affinity of the oil (camellia seed oil) for plant tissue (lavender flowers). The combination of these two effects promotes the permeability of the oil (camellia seed oil) to plant cells (lavender flowers), significantly increasing the release rate of lavender essential oil. This invention combines aqueous-phase plant fermentation technology with oil-phase plant extraction technology to obtain a highly efficient method for obtaining a fermented composition of lavender flowers and camellia seed oil. Experiments have verified that the mixed fermentation of lavender flowers and camellia seed oil has a synergistic effect, and the product has good anti-inflammatory and moisturizing effects, making it very suitable for use in anti-inflammatory or moisturizing products.

[0043] Optionally, the bumblebee *Saccharomyces cerevisiae* used for fermentation is classified as: *Saccharomyces cerevisiae* RTNY120013 Starmerella bombicola RTNY120013 is deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, China. The deposit date is August 12, 2025, and the accession number is CCTCC NO:M 20251820.

[0044] Specifically, the preparation method is as follows:

[0045] S1. Bumblebee *Saccharomyces cerevisiae* seed culture was cultured in YPD medium until OD... 600nm ≥10.

[0046] Yeast activation and fermentation medium (YPD medium):

[0047] Add 10 g / L yeast powder, 20 g / L peptone, and 20 g / L glucose to a final volume of 1000 mL with water, set the pH to 6.5, and sterilize at 121°C for 20 min.

[0048] S2. Crush the dried lavender flowers and pass them through a 100-mesh sieve. Then mix them evenly with camellia seed oil at a mass ratio (flower: oil) of 1:5~20. Sterilize at 105℃ for 15 minutes to obtain a sterilized flower-oil mixture.

[0049] Preferably, the ratio of flower oil to plant oil is 1:8 to 15, further 1:10 to 12, and more preferably 1:10.

[0050] S3. Prepare YPD medium and sterilize it at 120℃ for 15 minutes. After cooling to 30℃, inoculate with *Saccharomyces cerevisiae* seed culture at a rate of 5% (v / v). Incubate until OD500. 600nm ≥50, to obtain yeast fermentation broth.

[0051] S4. Add the sterilized flower oil mixture obtained in S2 to the bumblebee strophanthiozoon fermentation broth obtained in S3, and continue fermentation for 40-56 hours, wherein the volume ratio of bumblebee strophanthiozoon fermentation broth to sterilized flower oil mixture is 1-5:1-5.

[0052] Preferably, the volume ratio of bumblebee yeast fermentation broth to sterilized flower oil mixture is 1~4:1~2, more preferably 1:2.

[0053] S5. After fermentation is terminated, the fermentation products are separated, and the upper oil phase is retained.

[0054] S6. The oil phase is filtered to remove impurities and centrifuged to dehydrate, so as to obtain the desired fermentation product of lavender flower and camellia seed oil.

[0055] The method provided by this invention organically combines aqueous-phase plant fermentation technology and oil-phase plant extraction technology to achieve synergistic fermentation of camellia seed oil and lavender flowers, thereby improving the extraction efficiency of lavender essential oil. This extraction process is green and efficient, requiring no large-scale equipment investment or high energy consumption. The product has significant anti-inflammatory and moisturizing effects and can be applied to highly effective moisturizing cosmetics.

[0056] The fermentation product of lavender flowers and camellia seed oil provided in this embodiment of the invention is prepared using the preparation method provided in this invention.

[0057] This invention provides a moisturizing product comprising the fermentation products of lavender flowers and camellia seed oil provided in this invention.

[0058] Optionally, in order to enable moisturizing products to have better moisturizing effects at a lower cost, the content of fermentation products in moisturizing products is 0.1~3%wt.

[0059] This invention provides an anti-inflammatory product comprising the fermentation products of lavender flowers and camellia seed oil provided in this invention.

[0060] Example 1

[0061] 1. Bumblebee *Saccharomyces cerevisiae* seed culture (deposited at the China Center for Type Culture Collection, accession number CCTCC NO: M 20251820) was cultured on YPD medium until OD500. 600nm It is 15;

[0062] 2. Crush dried lavender flowers and pass them through a 100-mesh sieve. Then mix them with camellia seed oil at a mass ratio (flower: oil) of 1:10. Sterilize the mixture at 105℃ for 15 minutes to obtain a sterilized flower-oil mixture.

[0063] 3. Prepare YPD medium and sterilize at 120℃ for 15 minutes. After cooling to 30℃, inoculate with *Saccharomyces cerevisiae* seed culture at an inoculation rate of 5% (v / v) and culture until OD500. 600nm The concentration was 65, and the yeast fermentation broth was obtained.

[0064] 4. Add the sterilized flower oil mixture obtained in step 2 to the bumblebee yeast fermentation broth obtained in step 3, and continue fermentation for 48 hours. The volume ratio of the sterilized flower oil mixture to the yeast fermentation broth is 1:2.

[0065] 5. After fermentation is terminated, the fermentation products are separated, and the upper oil phase is retained;

[0066] 6. The oil phase is filtered to remove impurities and centrifuged to dehydrate, yielding the fermentation product (fermentation composition) of lavender flower and camellia seed oil.

[0067] Example 2

[0068] This embodiment is basically the same as embodiment 1, except that the mass ratio of dried lavender flowers to camellia seed oil is 1:5.

[0069] Example 3

[0070] This embodiment is basically the same as Embodiment 1, except that the mass ratio of dried lavender flowers to camellia seed oil is 1:20.

[0071] Example 4

[0072] This embodiment is basically the same as Embodiment 1, except that the volume ratio of bumblebee yeast fermentation liquid to flower oil mixture is 1:5.

[0073] Example 5

[0074] This embodiment is basically the same as Embodiment 1, except that the volume ratio of bumblebee yeast fermentation liquid to flower oil mixture is 5:1.

[0075] Example 6

[0076] This embodiment is basically the same as that of embodiment 1, except that the bumblebee strophanthis yeast sold by the China General Microbiological Culture Collection Center (CGMCC 2.3667) is used instead of the bumblebee strophanthis yeast in embodiment 1.

[0077] Comparative Example 1

[0078] The camellia seed oil used as a raw material in Example 1 was not fermented.

[0079] Comparative Example 2

[0080] This comparative example is basically the same as Example 1, except that the flower oil mixture in Example 1 is replaced with an equal amount of camellia seed oil.

[0081] Comparative Example 3

[0082] This comparative example is basically the same as Example 1, except that after obtaining the sterilized flower oil mixture, an equal amount of pure water was used instead of YPD medium, and no microorganisms were inoculated. The mixture was stirred continuously for 48 hours.

[0083] Experimental Example 1

[0084] Comparison of anti-inflammatory effects:

[0085] (1) Immunocytotoxicity test

[0086] Mouse monocytes / macrophages (RAW264.7) are commonly used model cells for validating the expression of inflammatory factors in immune cells. Succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT (thiazolyl blue) to water-insoluble blue-purple formazan crystals, which are then deposited in the cells. The lower the cell viability, the worse this function. Therefore, the MTT assay can be used to determine the effect of different drugs on cell viability, thereby characterizing drug toxicity to cells.

[0087] The concentration of RAW264.7 cells in the logarithmic growth phase was adjusted to 10 using DMEM medium. 4 Cells / mL: 100 μL of cell suspension was added to each well of a 96-well plate and cultured for 12 hours. A certain amount of sample solution diluted with DMSO was added to each well, and the plates were cultured for another 12 hours. The culture medium was then completely discarded. Subsequently, culture medium containing 0.5 mg / mL MTT was added to each well, and the cells were cultured for another 3-5 hours until crystals formed. The culture medium was then completely discarded, and 150 μL of DMSO was added to each well. The plates were gently shaken at room temperature for 5 minutes to completely dissolve the crystals. The 96-well plate was then quickly removed, and the absorbance of each well was measured at 490 nm using a microplate reader.

[0088] Experimental results: (e.g.) Figure 1 As shown, by examining Examples 1-6 under different fermentation process conditions, it can be found that each example exhibits essentially no cytotoxicity at concentrations of 0.01% and below, and cell viability can be maintained above 80%. Figure 2 As shown, by examining Example 1 and Comparative Examples 1-3, it can be found that Example 1 and Comparative Examples 1-3 exhibit essentially no cytotoxicity at concentrations of 0.01% and below, and cell viability can be maintained above 80%. Based on the above cytotoxicity test results, tests were conducted to inhibit the expression of inflammatory factors in immune cells.

[0089] (2) Inhibit NO expression in immune cells

[0090] NO is an important inflammatory signaling molecule, and its secretion level can reflect the intensity of inflammation. RAW264.7 macrophages secreted 2 × 10⁻⁶ NO molecules. 4Seeds were planted at a density of 2 mL / well in 6-well plates and incubated for 18 h in a 5% CO2, 37°C biochemical incubator. The culture medium was aspirated and residual medium was washed away with PBS. 2 mL of DMEM was added to the blank group, 1 μg / mL LPS prepared in DMEM was added to the control group, and 1 μg / mL LPS prepared in DMEM and sample solution diluted with DMSO were added to the sample groups. All samples were co-incubated for 24 h. The supernatant was collected, centrifuged at 13000 rpm for 5 min, and the supernatant was collected. Following the manufacturer's instructions, the effect of each sample on LPS-induced inflammatory cytokine expression was determined using a NO detection kit.

[0091] Experimental results: such as Figure 3 As shown, compared to the control group, Example 1 significantly inhibited the expression of the inflammatory factor NO in cells within the concentration range of 0.001-0.01%, demonstrating anti-inflammatory efficacy. At the same concentration (0.01%), the anti-inflammatory efficacy of Example 1 was superior to that of Examples 2-6, especially Example 1, which was superior to Example 6. This indicates that the novel *Saccharomyces simonii* strain deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 20251820 exhibits better anti-inflammatory effects than the products obtained from fermentation of existing *Saccharomyces simonii* strains. Figure 4 As shown, at the same concentration (0.00001%), the anti-inflammatory efficacy of Example 1 was superior to that of Comparative Examples 1-3.

[0092] Experiment Example 2

[0093] Comparison of moisturizing effects:

[0094] (1) Keratinocyte toxicity test

[0095] Keratinocytes (HaCaT) are commonly used model cells for validating skin efficacy. Succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT (thiazolyl blue) to water-insoluble blue-violet formazan crystals, which are then deposited in the cells. The lower the cell viability, the worse this function. Therefore, the MTT assay can be used to determine the effect of different drugs on cell viability, thereby characterizing drug toxicity to cells.

[0096] The concentration of HaCaT cells in the logarithmic growth phase was adjusted to 10 using DMEM medium. 4Cells / mL: 100 μL of cell suspension was added to each well of a 96-well plate and cultured for 12 hours. A certain amount of sample solution diluted with DMSO was added to each well, and the plates were cultured for another 12 hours. The culture medium was then completely discarded. Subsequently, culture medium containing 0.5 mg / mL MTT was added to each well, and the cells were cultured for another 3-5 hours until crystals formed. The culture medium was then completely discarded, and 150 μL of DMSO was added to each well. The plates were gently shaken at room temperature for 5 minutes to completely dissolve the crystals. The 96-well plate was then quickly removed, and the absorbance of each well was measured at 490 nm using a microplate reader.

[0097] Experimental results: such as Figure 5 As shown, Examples 1 and Comparative Examples 1-3 exhibited essentially no cytotoxicity at concentrations of 0.01% and below, maintaining cell viability above 80%. Based on the above cytotoxicity test results, the expression of AQP-3 in HaCaT cells was tested.

[0098] (2) Promotes AQP-3 expression in keratinocytes

[0099] Aquaporin 3 (AQP-3) is a recognized functional protein that maintains skin's moisturizing ability. Belonging to the water-glycerol channel protein subfamily, it functions to transport small molecules such as water, glycerol, and urea across the membrane. Promoting the expression of AQP-3 in skin cells can achieve effective skin hydration.

[0100] Lysis buffer preparation: Add 10 μL PMSF to 1 mL of strong RIPA lysis buffer and mix thoroughly.

[0101] HaCaT cells were grown in complete culture medium (high glucose DMEM medium containing 10% FBS and 1% penicillin antibiotics). Cells were digested with trypsin for 6 min (depending on trypsin activity, digestion continued until visible cell detachment was observed), at a rate of 2 × 10⁶ cells / min. 5 Two cells were seeded per well in a 6-well plate, 2 mL per well, and incubated at 37 °C in a 5% CO2 incubator for 18 h. The culture medium was discarded, and the cells were washed three times with PBS. 2 mL of 25 μg / mL SDS aqueous solution was added (this treatment was not performed on the blank group), and the cells were incubated for 4 h, followed by three more washes with PBS. Subsequently, 2 mL of DMEM medium was added to the control group, and 2 mL of sample solution diluted with DMSO and DMEM medium was added to the sample groups. The cells were incubated for 24 h. Finally, the culture medium was removed, and the cells were washed 2–3 times with pre-cooled PBS. 100 μL of lysis buffer was added to each well, and the cells were lysed on ice, ensuring the lysis buffer was in full contact with the cells for 5–10 seconds. The lysis buffer was collected, centrifuged at 10,000 rpm for 10 min, and the supernatant was used to detect AQP-3 levels using an ELISA assay.

[0102] Experimental results: such as Figure 6 As shown, compared with the blank group, the expression level of AQP-3 in HaCaT cells after SDS damage in the control group was significantly reduced. The sample from Example 1 significantly restored and enhanced the expression of AQP-3 in HaCaT cells, exhibiting a dose-dependent relationship. Furthermore, at the same addition amount (0.01%), Example 1's ability to promote AQP-3 expression in cells was significantly superior to that of Comparative Examples 1-3.

[0103] Experimental Example 3

[0104] (1) A moisturizing cream was formulated using water (blank group), fermented composition (Example 1), camellia seed oil (Comparative Example 1), fermented camellia seed oil (Comparative Example 2), and camellia seed oil extract of lavender flower (Comparative Example 3) as active ingredients for human body moisturizing efficacy testing. The moisturizing cream formula is shown in Table 1.

[0105] Table 1 Moisturizing Cream Formula

[0106]

[0107] (2) Moisturizing effect of a moisturizing cream containing a combination of fermented lavender flower and camellia seed oil.

[0108] The moisturizing effect of cosmetics on the human body can be verified by testing the water content of the stratum corneum and the transepidermal water loss.

[0109] The measurement area should be marked on the inner side of the subject's forearm. Before the formal test, the subject should sit quietly in a standard room for at least 30 minutes with the forearm exposed and placed in the testing position, remaining relaxed. The inner forearm should be marked with a 3×3cm area. 2 In the experimental area, multiple areas could be marked simultaneously on the same arm, with a 1cm interval between areas. Subsequently, approximately 0.02 g of a moisturizing cream formulation containing 2% water, a combination of fermented lavender flower and camellia seed oil (Example 1), fermented camellia seed oil (Comparative Example 2), unfermented camellia seed oil (Comparative Example 1), and camellia seed oil extract of lavender flower (Comparative Example 3) was applied to different areas of the inner forearm. The sample was evenly applied to the experimental area using a latex finger cot. The stratum corneum moisture content and transepidermal water loss were measured using a multifunctional skin analyzer before application (0h), 0.5h after application, 2h after application, and 4h after application.

[0110] Experimental results: such as Figure 7 and Figure 8 As shown, compared with the water-added formulation, the moisturizing cream with added oil has a significantly improved moisturizing ability. Among them, the moisturizing cream containing a combination of fermented lavender flower and camellia seed oil (Example 1) has the best effect on increasing the moisture content of the stratum corneum, and also has the greatest ability to inhibit skin moisture loss.

[0111] In summary, the preparation method provided in this embodiment of the invention involves mixing lavender flowers and camellia seed oil and fermenting them with bumblebee Saccharomyces cerevisiae. The mixed fermentation of lavender flowers and camellia seed oil has a synergistic effect, and the product has good anti-inflammatory and moisturizing effects, which can be used in anti-inflammatory or moisturizing products.

[0112] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing a fermentation product of lavender flowers and camellia seed oil, characterized in that, include: The flower oil mixture was fermented using bumblebee strophanthiozyme, and the oil phase product was collected after fermentation. The flower oil mixture is a mixture of lavender flower oil and camellia seed oil; the lavender flowers are dried lavender flowers, and the mass ratio of the dried lavender flowers to the camellia seed oil is 1:10; the bumblebee *Saccharomyces cerevisiae* is classified and named as: *Saccharomyces cerevisiae* RTNY120013. Starmerella bombicola RTNY120013, deposited at the China Center for Type Culture Collection, accession number CCTCC NO:M 20251820; Fermentation methods include: OD 600nm The bumblebee *Saccharomyces cerevisiae* fermentation broth with a concentration of ≥50% is mixed with the flower oil mixture and fermented for 40-56 hours, wherein the volume ratio of the bumblebee *Saccharomyces cerevisiae* fermentation broth to the flower oil mixture is 2:

1.

2. The preparation method according to claim 1, characterized in that, OD 600nm Prior to fermentation of ≥50g of bumblebee Saccharomyces cerevisiae ferment broth with the flower oil mixture, the following were also included: Bumblebee *Saccharomyces cerevisiae* seed culture was cultured in sterilized YPD medium until OD2000. 600nm ≥10; The yeast seed culture was inoculated into sterilized YPD medium and cultured until OD... 600nm The bumblebee yeast fermentation broth was obtained by ≥50.

3. The preparation method according to claim 1, characterized in that, The lavender flowers in the flower oil mixture are dried lavender flower powder that has passed through a 100-mesh sieve.

4. A fermentation product of lavender flowers and camellia seed oil, characterized in that, It is prepared by the preparation method described in any one of claims 1 to 3.

5. A moisturizing product, characterized in that, This includes the fermentation products of lavender flowers and camellia seed oil as described in claim 4.

6. The moisturizing product according to claim 5, characterized in that, The content of the fermentation product is 0.1~3%wt.

7. An anti-inflammatory product, characterized in that, This includes the fermentation products of lavender flowers and camellia seed oil as described in claim 4.

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