Strain incubator with waste recovery function and working method thereof

Abstract

The invention discloses a strain incubator with a waste recovery function and a working method thereof. The cultivation box comprises a box body and a control system, and a cultivation cavity and a gas passage system connected with the cultivation cavity are arranged in the box body. The gas passage system is composed of a gas inlet pipeline, a circulating pipeline and a gas exhaust pipeline, and each pipeline is provided with a control valve, an air filter, a heater and a circulating fan. A control system is used for switching on and off of a valve, and conversion between a cultivation mode and a processing mode is achieved. In the cultivation mode, sterile fresh air is introduced for strain growth. The treatment mode is converted into closed internal circulation, and the waste is heated by a heater to be subjected to high-temperature aerobic decomposition. The device integrates cultivation and waste treatment functions, realizes full-process automatic closed operation, effectively solves the problem of secondary pollution, greatly improves the treatment efficiency, and is particularly suitable for harmless and resourceful treatment of edible mushroom residues and solid fermentation inoculant carriers.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, and in particular to a microbial culture box with waste recycling function and its working method. Background Technology

[0002] Microbial cultivation technology is widely used in agriculture (such as edible fungi cultivation), environmental protection (such as the preparation of functional microbial agents), food, and biopharmaceutical industries. Currently, common microbial incubation boxes typically consist of a chamber with temperature, humidity, and gas exchange control, and their core function is to provide optimal environmental conditions for the growth of specific microorganisms.

[0003] However, existing microbial incubation boxes have limited functionality and generally suffer from the following technical defects:

[0004] (1) Challenges in handling cultivation waste: After a cultivation cycle, a large amount of solid culture medium waste (such as substrate residue) is generated. This waste is rich in organic matter, and if it is discarded at will, it will cause environmental pollution. If it is to be utilized as a resource (such as composting), the waste needs to be manually removed from the cultivation box and transported to a dedicated composting site or equipment for processing. This process is time-consuming and labor-intensive, increasing labor costs and operational complexity.

[0005] (2) High risk of secondary pollution: During the process of clearing and transferring waste, the cultivated waste is completely exposed to the external environment, which is very easy to breed bacteria, produce odors and emit dust. This not only pollutes the production workshop environment, but may also pose a biosafety threat to the subsequent new round of cultivation process, leading to bacterial contamination.

[0006] (3) Equipment idleness and energy waste: The microbial incubation box is a highly efficient environmental control unit, but it only plays a role in the incubation stage. In the waste treatment stage, additional composting equipment (such as trough turners and fermenters) needs to be started and energy is provided, resulting in repeated consumption of equipment investment and energy, and failing to maximize the utilization of equipment functions.

[0007] (4) Low processing efficiency: Traditional outdoor composting or external equipment processing has a long cycle (usually several weeks to several months), occupies a large area, and the processing effect is greatly affected by external environmental factors, resulting in unstable quality.

[0008] Therefore, there is an urgent need in this field for an integrated equipment that can combine microbial cultivation with in-situ waste treatment to solve the above-mentioned technical pain points and realize fully automated and closed operation from cultivation to waste resource utilization. Summary of the Invention

[0009] Purpose of the invention: The purpose of this invention is to provide a microbial incubation box with waste recycling function and its working method.

[0010] Technical solution:

[0011] A microbial culture box with waste recycling function includes a box body and a control system. The box body is equipped with a culture chamber, and the box body is connected to a gas passage system. The gas passage system is key to enabling the switching between culture and processing modes in this invention. By changing the gas flow path, it meets the differentiated needs for oxygen supply and heat circulation at different stages. The gas passage system includes:

[0012] The air intake pipe has one end connected to the outside air and the other end connected to the chamber. The air intake pipe is equipped with a first control valve and an air filter. The air intake pipe is responsible for supplying fresh air to the chamber during cultivation mode. The first control valve is used for on / off control, while the air filter effectively removes bacteria and dust from the air, preventing contamination during the cultivation process and ensuring pure culture of the bacteria.

[0013] The circulation pipeline, with its inlet and outlet connected to the top and bottom of the housing respectively, forms a closed loop. The circulation pipeline is equipped with a second control valve, a heater, and a circulating fan; it is the core component of the processing mode. The circulating fan drives the air within the chamber to form a closed-loop circulation, ensuring even heat distribution and preventing localized overheating or undercooling. The heater heats the circulating air to maintain the temperature required for high-temperature curing. The second control valve controls the on / off state of this pipeline.

[0014] The exhaust pipe, with its inlet end connected to the top of the chamber, is equipped with a third control valve. In cultivation mode, the exhaust pipe removes waste gases such as CO2 and excess moisture produced by the microbial respiration, maintaining a fresh gaseous environment inside the chamber. The third control valve controls the on / off state of this pipe.

[0015] Furthermore, at least one layer of perforated shelf is horizontally arranged within the cultivation chamber, driven to rotate by a drive motor via a rotating shaft. The rotatable perforated shelf is used to hold cultivation containers or solid culture media. Its rotation, driven by the drive motor via a rotating shaft, acts as a stirring mechanism in processing mode, breaking up material clumps and ensuring more uniform contact with air and processing agents, significantly improving composting efficiency and quality.

[0016] Furthermore, the housing also includes a spraying mechanism, which comprises a storage tank, a delivery pump, and an atomizing nozzle. The inlet of the delivery pump is connected to the storage tank via a pipeline, and the outlet is connected to the atomizing nozzle via a pipeline. The atomizing nozzle is located at the top of the cultivation chamber. The delivery pump is electrically connected to the control system. The spraying mechanism is used to precisely control the humidity within the chamber and add treatment agents. The storage tank stores water or liquid bacterial agent, the delivery pump provides the delivery power, and the atomizing nozzle atomizes the liquid and sprays it evenly to ensure uniform humidification or inoculation. All actions are intelligently controlled by the control system.

[0017] Furthermore, the bottom of the cultivation chamber is an inclined guide plate, with a discharge port at its lowest point. An electrically controlled discharge valve is installed at the discharge port and is electrically connected to the control system. The inclined guide plate facilitates the collection of the processed dried material to the discharge port by gravity. The electrically controlled discharge valve is opened by a command from the control system, achieving automated and sealed discharge of the processed material, eliminating the need for manual cleaning, and ensuring high efficiency and hygiene.

[0018] Furthermore, the control system is electrically connected to the first control valve, the second control valve, the third control valve, the heater, and the circulating fan. The incubator also includes a temperature sensor and a humidity sensor installed inside the chamber, which are electrically connected to the control system. The temperature and humidity sensors monitor the environmental conditions inside the chamber in real time and feed the data back to the control system. Based on this, the control system issues commands to precisely control the coordinated operation of various actuators, such as the control valves, heater, and circulating fan, to achieve closed-loop feedback control of the environment and ensure that the process conditions for cultivation and processing are always at their optimal state.

[0019] Furthermore, the control system is configured to execute a breeding mode or a processing mode:

[0020] Cultivation Mode: The first and third control valves are opened, while the second control valve is closed, allowing outside air to enter the chamber after being filtered by an air filter and then discharged through the exhaust pipe. In this mode, the system forms an open path of intake-recirculation-exhaust, continuously providing fresh, sterile, oxygen-rich air for bacterial growth and expelling metabolic waste gases, simulating an optimal growth environment. Gas flow can be driven by positive pressure in the intake pipe or by a fan on the pipe.

[0021] Processing Mode: The first and third control valves are closed, the second control valve is opened, and the heater and circulating fan are activated, causing the gas to circulate within a closed loop formed by the circulation pipeline and the housing. In this mode, the system transforms into a closed internal circulation system. The circulating fan drives the airflow, and the heater continuously heats the air. Heat accumulates within the system, rapidly and uniformly raising the internal temperature of the chamber to the preset high-temperature decomposition temperature, achieving efficient and energy-saving waste treatment.

[0022] This invention also discloses a method for operating a microbial incubation box with waste recycling function, including the following steps:

[0023] S1: Cultivation Stage: The control system executes the cultivation mode and controls the environmental parameters within the cultivation chamber to meet the growth requirements of the strain until cultivation is complete. The core of this stage is to provide the strain with the most suitable growth conditions by controlling parameters such as temperature, humidity, and ventilation to promote the efficient generation of mycelium or target metabolites.

[0024] S2: Mode switching: After cultivation is completed, the control system closes the first and third control valves and opens the second control valve; this step is the trigger point for function switching. By switching the opening and closing status of valves on each pipeline, the gas path is prepared for switching from the growth environment to the decomposition environment.

[0025] S3: Waste Treatment Stage: The control system activates the heater and circulating fan to raise and maintain the temperature within the cultivation chamber according to a preset waste treatment process curve, performing high-temperature aerobic composting on the cultivated waste. This stage is the core of waste resource utilization. The system utilizes its own equipment to perform high-temperature aerobic fermentation on the waste, killing pathogens and weed seeds, degrading easily decomposable organic matter, and rapidly stabilizing and rendering it harmless, transforming it into safe organic fertilizer.

[0026] S4: Discharge Stage: After waste processing is completed, the control system opens the electrically controlled discharge valve to discharge the processed material. This step completes the fully automated closed-loop process from feeding to discharging. The processed, stable product is discharged, and the equipment is ready for the next production cycle.

[0027] Furthermore, in step S3, the control system also activates the delivery pump of the spraying mechanism to spray water or composting agent into the cultivation chamber; and controls the drive motor to rotate or swing the perforated shelf. This optimized step significantly improves the processing effect. Spraying water can adjust the humidity of the compost pile to the optimal range of approximately 50% to 60% for composting microorganisms; spraying a special composting agent can accelerate the decomposition process. Driving the shelf to rotate enables automatic turning of the compost pile, solving the problem of overheating in the center and insufficient temperature on the periphery in static composting, making the material compost faster and more uniform.

[0028] Furthermore, the waste treatment process curve includes raising the temperature inside the cultivation chamber from room temperature to 60-75°C within 3-5 hours and maintaining this temperature for 24-72 hours. This process curve represents optimized, highly efficient composting parameters. Rapid heating quickly inhibits unwanted bacteria and activates the activity of thermophilic microorganisms. Maintaining a high temperature of 60-75°C is sufficient to kill most pathogens and weed seeds, while ensuring vigorous metabolism of thermophilic aerobic microorganisms, thereby achieving thorough composting and harmlessness of the waste in a relatively short time.

[0029] Furthermore, the strain is an edible fungal strain or a solid-state fermentation functional microbial agent strain. This invention is particularly suitable for treating the large amount of substrate waste generated after the cultivation of edible fungi such as shiitake and oyster mushrooms, or the carrier waste containing mycelia after the solid-state fermentation production of functional microbial agents such as Trichoderma and Bacillus, realizing on-site and value-added treatment of agricultural and bio-industrial waste.

[0030] Beneficial effects:

[0031] 1. Achieves integrated and automated cultivation and processing: This invention, through an innovative gas passage system design, enables the same equipment to intelligently switch between "cultivation mode" and "processing mode" by controlling the opening and closing of valves on different pipelines. After cultivation, the waste processing program can be automatically started without moving the materials, achieving seamless closed-loop operation, greatly reducing manual intervention and improving production efficiency.

[0032] 2. Eliminate secondary pollution and ensure biosafety: The entire cultivation and waste treatment process is completed in a closed cultivation chamber, which eliminates the pollution of the environment and operating area by bacteria, dust and odor caused by waste during the transfer process. It is especially suitable for industrial production scenarios with high cleanliness requirements.

[0033] 3. Energy saving and consumption reduction, improving equipment utilization: This invention makes full use of the existing heater, circulating fan and environmental control system of the incubator. In the processing mode, the air is internally heated and circulated through the circulation pipeline, which greatly reduces energy consumption, avoids the cost of adding additional composting equipment, and realizes the reuse of equipment functions and maximizes its value.

[0034] 4. Improve waste treatment efficiency and quality: The programmable control system precisely controls the temperature curve of the high-temperature composting process (e.g., maintaining 60-75℃ for 24-72 hours), creating the best conditions for high-temperature aerobic fermentation. It can complete the composting process that traditional composting takes several weeks in a short time (within a few days), with extremely high treatment efficiency and stable and thoroughly harmless products.

[0035] 5. Strong functional expandability and better treatment effect: By integrating a spraying mechanism and a rotatable hollow shelf, water or microbial agents can be automatically added during the treatment process, and uniform mixing can be carried out to ensure the uniformity and efficiency of waste decomposition, further improving the quality of the final product.

[0036] 6. Specific application: This invention is particularly suitable for the treatment of substrate after edible fungi cultivation and the treatment of carriers after the production of solid-state fermentation functional microbial agents (such as Trichoderma and Bacillus). It is highly targeted, solves practical problems in these specific fields, and has broad market application prospects. Attached Figure Description

[0037] Figure 1 This is a perspective view of the present invention.

[0038] Figure 2 This is a perspective view of the invention from another angle.

[0039] Figure 3 This is the three-dimensional form of the sectional state of the present invention. Figure 1 ;

[0040] Figure 4This is the three-dimensional form of the sectional state of the present invention. Figure 2 ;

[0041] Figure 5 This is a flowchart of the working method of the present invention. Detailed Implementation

[0042] To make the technical solution of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0043] Example 1

[0044] like Figure 1-4 As shown, a microbial culture box with waste recycling function includes a box body 1 and a control system. A culture chamber 2 is provided inside the box body. The box body 1 is connected to a gas passage system 3. The gas passage system 3 is key to the switching between culture and processing modes in this invention. By changing the gas flow path, it meets the differentiated needs for oxygen supply and heat circulation at different stages. The gas passage system 3 includes:

[0045] An air intake pipe 31 is connected to the outside air at one end and to the chamber 1 at the other end. The air intake pipe 31 is equipped with a first control valve 311 and an air filter 312. The air intake pipe 31 is responsible for supplying fresh air to the chamber 1 during the cultivation mode. The first control valve 311 is used for on / off control, while the air filter 312 effectively removes bacteria and dust from the air, preventing contamination during the cultivation process and ensuring pure culture of the bacteria.

[0046] The circulation pipe 32, with its inlet and outlet ends connected to the top and bottom of the housing 1 respectively, forms a closed loop. The circulation pipe is equipped with a second control valve 321, a heater 322, and a circulating fan 323; the circulation pipe 32 is the core component of the processing mode. The circulating fan 323 drives the air within the chamber to form a closed-loop circulation, ensuring uniform heat distribution and preventing localized overheating or undercooling. The heater 322 heats the circulating air to maintain the temperature required for high-temperature curing. The second control valve 321 controls the on / off state of this pipe.

[0047] The exhaust pipe 33 has its inlet end connected to the top of the chamber 1, and a third control valve 331 is installed on it. In cultivation mode, the exhaust pipe 33 discharges waste gases such as CO2 and excess water vapor generated by the respiration of the bacteria outside the chamber, maintaining a fresh gaseous environment inside the chamber. The third control valve 331 is used to control the opening and closing of this pipe.

[0048] Furthermore, at least one layer of perforated shelf 13, driven to rotate by a drive motor 11 via a rotating shaft 12, is horizontally arranged inside the cultivation chamber 2. The rotatable perforated shelf 13 is used to hold cultivation containers or solid culture media. Its rotation function is driven by the drive motor 11 via the rotating shaft 12, and in the processing mode, it can play a stirring role, breaking up material clumps, making it more uniformly contacted with air and processing agents, and significantly improving the ripening efficiency and quality.

[0049] Furthermore, the incubation chamber also includes a spraying mechanism 4, which comprises a storage tank 41, a delivery pump 42, and an atomizing nozzle 43. The inlet of the delivery pump 42 is connected to the storage tank 41 via a pipeline, and the outlet is connected to the atomizing nozzle 43 via a pipeline. The atomizing nozzle 43 is located at the top of the incubation chamber 2. The delivery pump 42 is electrically connected to the control system. The spraying mechanism 4 is used to precisely control the humidity within the chamber and add treatment agents. The storage tank 41 stores water or liquid bacterial agent, the delivery pump 42 provides the delivery power, and the atomizing nozzle 43 atomizes the liquid and sprays it evenly to ensure uniform humidification or inoculation. All actions are intelligently controlled by the control system.

[0050] Furthermore, the bottom of the cultivation chamber 2 is an inclined guide plate 21, with a discharge port 22 at its lowest point. An electrically controlled discharge valve 23 is installed at the discharge port 22 and is electrically connected to the control system. The inclined guide plate 21 facilitates the concentration of the processed dried material to the discharge port 22 by gravity. The electrically controlled discharge valve 23 is opened by the control system, achieving automated and sealed discharge of the processed material, eliminating the need for manual cleaning, thus ensuring high efficiency and hygiene.

[0051] Furthermore, the cultivation chamber and the drive motor are both fixedly mounted on the base. The base can slide and be fixed along the direction of the chamber's entry and exit by means of the bottom sliding groove and the slider at the bottom of the chamber, which facilitates loading and unloading.

[0052] Furthermore, the control system is electrically connected to the first control valve 311, the second control valve 321, the third control valve 331, the heater 322, and the circulating fan 323. The incubator also includes a temperature sensor 51 and a humidity sensor 52 disposed within the incubation chamber 2, which are electrically connected to the control system. The temperature sensor 51 and the humidity sensor 52 monitor the environmental conditions within the chamber in real time and feed the data back to the control system. Based on this, the control system issues commands to precisely control the coordinated operation of various actuators, such as the control valves, the heater 322, and the circulating fan 323, to achieve closed-loop feedback control of the environment and ensure that the process conditions for incubation and processing are always at their optimal state.

[0053] Furthermore, the control system is configured to execute a breeding mode or a processing mode:

[0054] Cultivation mode: The first control valve 311 and the third control valve 331 are opened, the second control valve 321 is closed, and the circulating fan 323 is started, so that the outside air enters the box 1 after being filtered by the air filter 312 and is discharged through the exhaust pipe 33. In this mode, the system forms an open path of air intake-circulation-exhaust, continuously providing fresh, sterile, oxygen-rich air for the growth of bacteria and discharging metabolic waste gas, simulating the optimal growth environment.

[0055] Processing Mode: The first control valve 311 and the third control valve 331 are closed, the second control valve 321 is opened, and the heater 322 and the circulating fan 323 are activated, causing the gas to circulate within the closed loop formed by the circulating pipeline 32 and the housing 1. In this mode, the system transforms into a closed internal circulation system. The circulating fan 323 drives the airflow, and the heater 322 continuously heats the air. Heat accumulates within the system, rapidly and uniformly raising the temperature inside the chamber to the preset high-temperature decomposition temperature, achieving efficient and energy-saving waste treatment.

[0056] Example 2

[0057] like Figure 5 As shown, the present invention also discloses a method for operating the above-mentioned microbial incubation box, including the following steps:

[0058] S1: Cultivation Stage: The control system executes the cultivation mode and controls the environmental parameters within the cultivation chamber 2 to meet the growth requirements of the strain until cultivation is complete. The core of this stage is to provide the strain with the most suitable growth conditions by controlling parameters such as temperature, humidity, and ventilation to promote the efficient generation of mycelium or target metabolites.

[0059] S2: Mode switching: After cultivation is completed, the control system closes the first control valve 311 and the third control valve 331 and opens the second control valve 321. This step is the trigger point for function switching. By switching the opening and closing status of the valves on each pipeline, the gas path is prepared for switching from the growth environment to the maturation environment.

[0060] S3: Waste Treatment Stage: The control system activates heater 322 and circulating fan 323 to raise and maintain the temperature inside the cultivation chamber 2 according to a preset waste treatment process curve, performing high-temperature aerobic composting treatment on the cultivated waste; this stage is the core of waste resource utilization. The system utilizes its own equipment to perform high-temperature aerobic fermentation on the waste, killing pathogens and weed seeds, degrading easily decomposable organic matter, and rapidly stabilizing and rendering it harmless, transforming it into safe organic fertilizer.

[0061] S4: Discharge Stage: After waste processing is completed, the control system opens the electrically controlled discharge valve 23 to discharge the processed material. This step completes the fully automated closed loop from feeding to discharging. The processed stable product is discharged, and the equipment is ready for the next production cycle. In this step, a waste collection box can be set at the bottom of the box to collect the waste directly. Alternatively, as in Example 1, the cultivation chamber and drive motor can be fixedly installed on the base. The base can slide and be fixed along the box's inlet / outlet direction via a bottom groove in conjunction with a slider at the bottom of the box. The cultivation chamber 2 can be moved outside the box for unloading by moving the base.

[0062] Furthermore, in step S3, the control system also activates the delivery pump 42 of the spraying mechanism 4 to spray water or composting agent into the cultivation chamber 2; and controls the drive motor 11 to operate, driving the hollowed-out shelf 13 to rotate or swing. This optimized step can significantly improve the processing effect. Spraying water can adjust the humidity of the compost pile to the optimal range of approximately 50% to 60% for composting microorganisms; spraying a special composting agent can accelerate the decomposition process. Driving the shelf 13 to rotate enables automatic turning of the compost pile, solving the problem of overheating in the center and insufficient temperature on the periphery in static composting, making the material compost faster and more uniform.

[0063] Furthermore, the waste treatment process curve includes raising the temperature inside the cultivation chamber 2 from room temperature to 60-75°C within 3-5 hours and maintaining this temperature for 24-72 hours. This process curve represents optimized, highly efficient composting parameters. Rapid heating quickly inhibits unwanted bacteria and activates the activity of thermophilic microorganisms. Maintaining a high temperature of 60-75°C is sufficient to kill most pathogens and weed seeds, while ensuring vigorous metabolism of thermophilic aerobic microorganisms, thereby achieving thorough composting and harmlessness of the waste in a relatively short time.

[0064] Furthermore, the strain is an edible fungal strain or a solid-state fermentation functional microbial agent strain. This invention is particularly suitable for treating the large amount of substrate waste generated after the cultivation of edible fungi such as shiitake and oyster mushrooms, or the carrier waste containing mycelia after the solid-state fermentation production of functional microbial agents such as Trichoderma and Bacillus, realizing on-site and value-added treatment of agricultural and bio-industrial waste.

[0065] One of the core innovations of this invention is that it is not a general waste treatment device, but is deeply adapted to the characteristics of waste generated by two specific processes: edible fungi cultivation and solid-state fermentation of functional microbial agents. This solves the industry pain point that existing technologies cannot efficiently, economically, and harmlessly treat such waste.

[0066] Example 3: Deep adaptation for edible mushroom substrate waste

[0067] The substrate residue produced after the cultivation of edible fungi such as shiitake, oyster, and enoki mushrooms is mainly composed of incompletely utilized lignocellulose matrix (such as sawdust, straw, and cottonseed hulls), abundant mycelial protein (mycelium), and metabolic products such as polysaccharides. It is characterized by its large volume, dense texture, high C / N ratio, and slow natural degradation.

[0068] Traditional fermentation methods involve a long fermentation cycle of 2-4 months, occupy a large amount of space, and are prone to the growth of miscellaneous bacteria and pests, causing environmental pollution. The high-value components (microbial protein, polysaccharides) in the fermented substrate are not effectively utilized.

[0069] The targeted solution of this invention:

[0070] Physical solution: The built-in rotatable perforated shelf 13 rotates at low speed in processing mode, effectively breaking up the clumps of compost and disrupting its dense structure. This greatly increases the contact area between the material and oxygen and the inoculant, creating the prerequisites for rapid composting. This is something that static composting cannot achieve.

[0071] Highly efficient composting: The gas passage system 3 rapidly raises the temperature to 60-75℃ and maintains it. This temperature range effectively kills residual edible fungal pathogens and weed seeds in the substrate, rendering them harmless. At the same time, the high temperature significantly accelerates the degradation rate of lignocellulose, shortening the traditional cycle of several months to less than 72 hours.

[0072] Resource value-added: The processed product is no longer "waste", but a stabilized and harmless high-quality organic fertilizer or bio-organic fertilizer substrate, realizing the resource value-added of waste.

[0073] Example 4: Deep adaptation of solid-state fermentation functional microbial agent carrier waste

[0074] Functional microbial agents, such as Trichoderma, Bacillus, and Aspergillus, typically use wheat bran, soybean meal, and rice husks as solid-state fermentation carriers. After fermentation, the carrier is rich in high concentrations of functional bacterial cells, unconsumed nutrients, and target metabolites (such as enzymes and antibiotics). This type of waste may have high salt content and pH levels; improper handling can easily lead to the spread of bacterial strains and pose ecological risks.

[0075] This type of waste is rich in live bacteria and cannot be disposed of indiscriminately. High-temperature sterilization followed by landfilling is costly and does not allow for resource recovery. Simple composting cannot ensure the complete inactivation of residual functional bacteria, posing environmental regulatory risks.

[0076] The targeted solution of this invention:

[0077] Absolutely harmless: The high-temperature composting mode of this system can ensure that the temperature penetrates the core of the material evenly, completely inactivating the residual functional strains (such as Trichoderma and Bacillus) in the carrier, completely eliminating the risk of environmental leakage of genetically engineered bacteria or dominant functional strains, and meeting the most stringent biosafety and environmental protection requirements.

[0078] Closed-loop treatment: The entire treatment process is completed within a completely sealed enclosure 1, with no leakage of any waste gas, dust, or live bacteria. It is particularly suitable for GMP workshops with high biosafety requirements or areas with strict environmental regulations.

[0079] Value Preservation and Transformation: While inactivating the bacteria, the treatment process retains the organic nutrients such as bacterial proteins and enzymes in the carrier to the greatest extent, transforming them into high-quality organic fertilizer rich in biological activity, thus achieving a remarkable transformation from "waste" to "product".

[0080] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A microbial incubation box with waste recycling function, characterized in that, Includes a housing (1) and a control system. The housing contains a cultivation chamber (2). The housing (1) is connected to a gas passage system (3), which includes: An air intake pipe (31) is connected to the outside air at one end and to the housing (1) at the other end. A first control valve (311) and an air filter (312) are provided on the air intake pipe (31). The circulation pipeline (32) has its inlet end and outlet end connected to the top and bottom of the box (1) respectively, forming a closed loop. The circulation pipeline is equipped with a second control valve (321), a heater (322) and a circulation fan (323); the exhaust pipeline (33) has its inlet end connected to the top of the box (1) and is equipped with a third control valve (331).

2. The microbial incubation box with waste recycling function according to claim 1, characterized in that, The cultivation chamber (2) is horizontally provided with at least one hollow shelf (13) that is driven to rotate by a drive motor (11) through a rotating shaft (12).

3. The microbial incubation box with waste recycling function according to claim 1, characterized in that, The incubation chamber also includes a spraying mechanism (4), which includes a liquid storage tank (41), a delivery pump (42), and an atomizing nozzle (43). The inlet of the delivery pump (42) is connected to the liquid storage tank (41) through a pipeline, and the outlet is connected to the atomizing nozzle (43) through a pipeline. The atomizing nozzle (43) is located on the top of the incubation chamber (2). The delivery pump (42) is electrically connected to the control system.

4. The microbial incubation box with waste recycling function according to claim 1, characterized in that, The bottom of the cultivation chamber (2) is an inclined guide plate (21). A discharge port (22) is opened at the lowest point of the guide plate (21). An electrically controlled discharge valve (23) is provided at the discharge port (22). The electrically controlled discharge valve (23) is electrically connected to the control system.

5. A microbial incubation box with waste recycling function according to claim 1, characterized in that, The control system is electrically connected to the first control valve (311), the second control valve (321), the third control valve (331), the heater (322), and the circulating fan (323); the incubator also includes a temperature sensor (51) and a humidity sensor (52) disposed in the chamber (1), and the temperature sensor (51) and the humidity sensor (52) are electrically connected to the control system.

6. The integrated intelligent equipment according to claim 5, characterized in that, The control system is configured to execute either a breeding mode or a processing mode: Cultivation mode: The first control valve (311) and the third control valve (331) are opened, and the second control valve (321) is closed, so that the outside air enters the box (1) after being filtered by the air filter (312) and is discharged through the exhaust pipe (33); Processing mode: Control the first control valve (311) and the third control valve (331) to close, the second control valve (321) to open, and start the heater (322) and the circulating fan (323) to make the gas circulate in the closed loop formed by the circulating pipeline (32) and the box (1).

7. A method for operating a microbial incubation box with waste recycling function, characterized in that, Includes the following steps: S1: Cultivation stage: The control system executes the cultivation mode and controls the environmental parameters in the cultivation chamber (2) to meet the growth requirements of the strain until cultivation is completed; S2: Mode switching: After cultivation is completed, the control system closes the first control valve (311) and the third control valve (331) and opens the second control valve (321); S3: Waste treatment stage: The control system starts the heater (322) and the circulating fan (323) to raise and maintain the temperature inside the box (1) according to a preset waste treatment process curve, and to carry out high-temperature aerobic composting treatment on the cultivated waste. S4: Discharge stage: After the waste is processed, the control system opens the electrically controlled discharge valve (23) to discharge the processed material.

8. The working method of a microbial incubation box with waste recycling function according to claim 7, characterized in that, In step S3, the control system also starts the delivery pump (42) of the spraying mechanism (4) to spray water or composting agent into the cultivation chamber (2); controls the drive motor (11) to run, driving the hollow shelf (13) to rotate or swing.

9. The working method of a microbial incubation box with waste recycling function according to claim 7, characterized in that, The waste treatment process curve includes: raising the temperature inside the cultivation chamber (2) from room temperature to 60-75°C within 3-5 hours, and maintaining it at that temperature for 24-72 hours.

10. The working method according to claim 7, characterized in that, The strains are edible fungi strains or solid-state fermentation functional microbial agents.

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