Method for extracting soybean lecithin from soybean phospholipid

Abstract

The invention relates to the technical field of biology, in particular to a method for extracting soybean lecithin from soybean phospholipid. The method comprises the following steps: carrying out first stirring treatment on a first mixture obtained by mixing soybean phospholipid and acetone to obtain a solid soybean phospholipid crude extract; carrying out second stirring treatment on a second mixture obtained by mixing the solid soybean phospholipid crude extract and ethanol to obtain a soybean phospholipid crude extract, and concentrating the soybean phospholipid crude extract to obtain a concentrated solution; carrying out enzymolysis on the concentrated solution by using a compound enzyme to obtain a soybean lecithin concentrated solution, the compound enzyme being composed of phospholipase A2, lipase and protease; and carrying out enzyme deactivation treatment and drying treatment on the soybean lecithin concentrated solution to obtain the soybean lecithin. According to the method, the yield and purity of the soybean lecithin can be effectively improved, and the extracted soybean lecithin has a high moisture retention rate and has a wide application prospect in the food industry.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and more particularly to a method for extracting soybean lecithin from soybean phospholipids. Background Technology

[0002] Lecithin is a group of yellowish-brown oily substances found in animal and plant tissues and egg yolks. Due to its excellent properties and various physiological functions, it has received widespread attention. Soy lecithin, a natural plant-derived lecithin extracted from soybeans, mainly consists of phosphatidylcholine, phosphatidylethanolamine, and small amounts of triglycerides and fatty acids. Because of its good emulsifying, moisturizing, and antioxidant properties, it is used in the food industry as an emulsifier, preservative, and anti-caking agent.

[0003] Currently, the main methods for extracting soybean lecithin include organic solvent extraction, column chromatography, ultrasonic extraction, and supercritical fluid extraction. Organic solvent extraction is the most commonly used, but the purity of the extracted soybean lecithin is relatively low. Column chromatography yields higher purity soybean lecithin, but it suffers from complex separation processes, long extraction times, and large amounts of elution solvent, making industrialization difficult. Ultrasonic extraction utilizes the cavitation effect of ultrasound to accelerate the extraction of soybean lecithin, while supercritical fluid extraction uses supercritical fluid as a solvent. However, both methods require specialized equipment, resulting in high investment costs, complex operations, and high production costs.

[0004] Therefore, how to increase the yield and purity of soybean lecithin while reducing production costs is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] This invention provides a method for extracting soybean lecithin from soybean lecithin. This method can effectively improve the yield and purity of soybean lecithin, and the extracted soybean lecithin has a high moisturizing rate, which has broad application prospects in the food industry. The method is simple to operate, does not require the use of special extraction equipment, can effectively reduce production costs, and is suitable for widespread application.

[0006] This invention provides a method for extracting soybean lecithin from soybean phospholipids, comprising:

[0007] The first mixture of soybean lecithin and acetone was subjected to a first stirring treatment to obtain a solid crude soybean lecithin extract.

[0008] The second mixture of the solid soybean lecithin crude extract and ethanol is subjected to a second stirring treatment to obtain a soybean lecithin crude extract. The soybean lecithin crude extract is then concentrated to a solid content of 45% to 55% to obtain a concentrated solution.

[0009] The concentrated solution is subjected to enzymolysis using a complex enzyme, wherein the complex enzyme is composed of phospholipase A2, lipase and protease.

[0010] The soybean lecithin concentrated solution is subjected to enzyme inactivation treatment and drying treatment, and the soybean lecithin is obtained.

[0011] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the mass ratio of the soybean phospholipid to acetone is 1:(1-20).

[0012] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the mass ratio of the solid soybean phospholipid crude extract to ethanol is 1:(1-20).

[0013] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the mass ratio of phospholipase A2, lipase and protease in the complex enzyme is 1:3:2.

[0014] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the mass ratio of the complex enzyme to the concentrated solution is 1:(80-120).

[0015] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the enzymolysis of the concentrated solution using the complex enzyme specifically comprises:

[0016] After the complex enzyme is mixed with the concentrated solution, the enzymolysis is performed at 40-50°C for 50-65 min.

[0017] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the rotating speed of the first stirring treatment is 80-120 r / min, and the time is 20-60 min.

[0018] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the rotating speed of the second stirring treatment is 150-250 r / min, and the time is 20-30 min.

[0019] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the temperature of the enzyme inactivation treatment is 80-100°C, and the time is 5-15 min.

[0020] The method for extracting soybean lecithin from soybean phospholipid as described above, wherein the temperature of the drying treatment is 45-50°C, and the time is 80-100 min.

[0021] Compared with the prior art, the present application has at least the following beneficial effects:

[0022] The application provides a method for extracting soybean lecithin from soybean phospholipids, which comprises the following steps: firstly, extracting soybean phospholipids by using acetone to obtain solid soybean phospholipid crude extract; secondly, extracting the solid soybean phospholipid crude extract by using ethanol to obtain a soybean phospholipid crude extract solution; thirdly, concentrating the soybean phospholipid crude extract solution to obtain a concentrated solution; fourthly, performing enzymolysis on the concentrated solution by using a composite enzyme composed of phospholipase A2, lipase and protease to obtain a soybean lecithin concentrated solution; and finally, performing enzyme inactivation treatment and drying treatment on the soybean lecithin concentrated solution to obtain the soybean lecithin. The method can effectively improve the yield and purity of the soybean lecithin, and the extracted soybean lecithin has high moisture retention rate and has a wide application prospect in the food industry. In addition, the method is simple to operate, does not need to use special extraction equipment, and has low production cost, and is suitable for wide application and promotion. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only some embodiments of the application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0024] Figure 1 The liquid chromatogram of the soybean lecithin in Example 1 is shown in the figure, wherein the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min.

[0025] Figure 2 The liquid chromatogram of the soybean lecithin in Example 2 is shown in the figure, wherein the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min.

[0026] Figure 3 The liquid chromatogram of the soybean lecithin in Example 3 is shown in the figure, wherein the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min.

[0027] Figure 4 The liquid chromatogram of the soybean lecithin in Example 4 is shown in the figure, wherein the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min.

[0028] Figure 5 The liquid chromatogram of the soybean lecithin in Example 5 is shown in the figure, wherein the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min.

[0029] Figure 6 The liquid chromatogram of the soybean lecithin in Example 6 is shown in the figure, wherein the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min.

[0030] Figure 7The liquid chromatogram of soybean lecithin in Comparative Example 1, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min;

[0031] Figure 8 The liquid chromatogram of soybean lecithin in Comparative Example 2, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min;

[0032] Figure 9 The liquid chromatogram of soybean lecithin in Comparative Example 3, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min;

[0033] Figure 10 The liquid chromatogram of soybean lecithin in Comparative Example 4, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min;

[0034] Figure 11 The liquid chromatogram of soybean lecithin in Comparative Example 5, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min;

[0035] Figure 12 The liquid chromatogram of soybean lecithin in Comparative Example 6, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min;

[0036] Figure 13 The liquid chromatogram of soybean lecithin in Comparative Example 7, the vertical coordinate is the electric signal intensity / mV, and the horizontal coordinate is the retention time / min. DETAILED DESCRIPTION

[0037] In order to make the objects, technical solutions and advantages of the present application clearer, the following will combine the embodiments of the present application to make a clear and complete description of the technical solutions in the embodiments of the present application. Obviously, the described embodiments are some embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0038] The raw materials and reagents used in the following embodiments can be obtained from commercial channels if no special instructions are given, and the processes used can be conventional processes in the art if no special instructions are given.

[0039] It should be noted that the descriptions of "first", "second" and the like in the present application are used to distinguish similar objects, and are not used to describe a specific order or sequence, and therefore should not be understood as a limitation on the present application.

[0040] In addition, "and / or" in the specification and claims indicates at least one of the connected objects, and the character " / " generally indicates that the front and rear associated objects are in an "or" relationship.

[0041] The present application provides a method for extracting soybean lecithin from soybean phospholipid, comprising:

[0042] The first mixture of the soybean phospholipid mixed with acetone is subjected to a first stirring treatment to obtain a solid soybean phospholipid crude extract;

[0043] The second mixture of the solid soybean phospholipid crude extract mixed with ethanol is subjected to a second stirring treatment to obtain a soybean phospholipid crude extract solution, and the soybean phospholipid crude extract solution is concentrated to a solid content of 45% to 55% to obtain a concentrated solution;

[0044] The concentrated solution is subjected to enzymatic hydrolysis using a composite enzyme to obtain a soybean lecithin concentrated solution, wherein the composite enzyme is composed of phospholipase A2, lipase and protease;

[0045] The soybean lecithin concentrated solution is subjected to enzyme inactivation treatment and drying treatment to obtain the soybean lecithin.

[0046] The present application does not particularly limit the specific sources of the raw materials and reagents used in the above method process, which can be purchased from commercial channels.

[0047] Specifically, the soybean phospholipid is mixed with acetone to obtain a first mixture, and then the first mixture is subjected to a first stirring treatment to fully mix and uniformly mix the soybean phospholipid and acetone, and after solid-liquid separation, the oil and fat is preliminarily removed to obtain a solid soybean phospholipid crude extract; then the solid soybean phospholipid crude extract is mixed with ethanol to obtain a second mixture, and the second mixture is subjected to a second stirring treatment to fully mix the solid soybean phospholipid crude extract and ethanol, and after solid-liquid separation, a soybean phospholipid crude extract solution is obtained, and then the soybean phospholipid crude extract solution is concentrated to a solid content of 45% to 55% to obtain a concentrated solution, and the concentration is to improve the concentration of lecithin, which is beneficial to subsequent enzymatic hydrolysis; then the composite enzyme composed of phospholipase A2, lipase and protease is mixed with the concentrated solution for enzymatic hydrolysis to obtain a soybean lecithin concentrated solution, and the enzymatic hydrolysis can purify lecithin and improve hydrophilicity; then the soybean lecithin concentrated solution is subjected to enzyme inactivation treatment to obtain the soybean lecithin concentrated solution after enzyme inactivation, and the enzyme inactivation treatment is to prevent deep hydrolysis of lecithin; finally, the soybean lecithin concentrated solution after enzyme inactivation is subjected to drying treatment to obtain the soybean lecithin, and the purpose of the drying treatment is to prevent oxidation.

[0048] The present application can effectively improve the extraction rate of soybean lecithin by the above method, and the extracted soybean lecithin has the advantages of high purity and high hydrophilicity; the method is simple to operate, does not need to use special extraction equipment, and has low production cost.

[0049] In a specific embodiment, the mass ratio of the above soybean phospholipid to acetone is 1:(1-20).

[0050] When the mass ratio of the soybean phospholipid to the acetone is within the above range, the yield and purity of the soybean lecithin can be improved.

[0051] Exemplarily, the mass ratio of the soybean phospholipid to the acetone can be any one or a range consisting of any two of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20.

[0052] In an embodiment, the mass ratio of the solid soybean phospholipid crude extract to the ethanol is 1:(1-15).

[0053] When the mass ratio of the solid soybean phospholipid crude extract to the ethanol is within the above range, the yield and purity of the soybean lecithin can be improved.

[0054] Exemplarily, the mass ratio of the solid soybean phospholipid crude extract to the ethanol can be any one or a range consisting of any two of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20.

[0055] In an embodiment, the mass ratio of the phospholipase A2, the lipase and the protease in the complex enzyme is 1:3:2.

[0056] The inventors have found that when the complex enzyme used is composed of the phospholipase A2, the lipase and the protease in a mass ratio of 1:3:2, the synergistic effect can be fully exerted, and the yield and purity of the soybean lecithin can be effectively improved.

[0057] In an embodiment, the mass ratio of the complex enzyme to the concentrated solution is 1:(80-120), preferably 1:100.

[0058] In an embodiment, the enzyme hydrolysis of the concentrated solution by the complex enzyme comprises:

[0059] After the complex enzyme is mixed with the concentrated solution, the enzyme hydrolysis is performed at 40-50°C for 50-65 min.

[0060] In an embodiment, the rotation speed of the first stirring treatment is 80-120 r / min, and the time is 20-60 min.

[0061] In a specific embodiment, the rotation speed of the second stirring treatment is 150 r / min to 250 r / min, and the time is 20 min to 30 min.

[0062] In a specific embodiment, the temperature of the enzyme inactivation treatment is 80℃ to 100℃, and the time is 5 min to 15 min.

[0063] In a specific embodiment, the temperature of the drying treatment is 45℃ to 50℃, and the time is 80 min to 100 min.

[0064] The application will be further described below through specific examples.

[0065] The raw material soybean phospholipid was purchased from Zhongchuang Engineering Technology Co., Ltd.; acetone was purchased from Wuhan Gao Hengsheng Technology Development Co., Ltd.; ethanol was purchased from Wuhan Gao Hengsheng Technology Development Co., Ltd.; phospholipase A2 was purchased from Shanghai Kanglang Biological Technology Co., Ltd.; lipase was purchased from Wuhan Xinhua Yang Biological Co., Ltd.; and protease was purchased from Wuhan Xinhua Yang Biological Co., Ltd.

[0066] Example 1

[0067] The present embodiment provides a method for preparing soybean lecithin, which comprises the following steps:

[0068] (1) 1 g of soybean phospholipid is mixed with 10 g of acetone to obtain a first mixture, and then the first mixture is subjected to a first stirring treatment at a rotation speed of 100 r / min for 30 min, and after solid-liquid separation, a solid soybean phospholipid crude extract is obtained;

[0069] (2) The solid soybean phospholipid crude extract in (1) is mixed with ethanol according to a mass ratio of 1:10 to obtain a second mixture, and the second mixture is subjected to a second stirring treatment at a rotation speed of 200 r / min for 25 min, and after solid-liquid separation, a soybean phospholipid crude extract is obtained; the soybean phospholipid crude extract is vacuum concentrated to a solid content of 50% to obtain a concentrated solution;

[0070] (3) The concentrated solution in (2) is mixed with a compound enzyme according to a mass ratio of 1:100, and then subjected to enzymolysis at 45℃ for 60 min to obtain a soybean lecithin concentrated solution; the compound enzyme is composed of phospholipase A2, lipase and protease according to a mass ratio of 1:3:2;

[0071] (4) The soybean lecithin concentrated solution in (3) is subjected to enzyme inactivation treatment at 85℃ for 10 min to obtain an enzyme-inactivated soybean lecithin concentrated solution; finally, the enzyme-inactivated soybean lecithin concentrated solution is subjected to drying treatment at 49℃ for 90 min to obtain 0.31 g of soybean lecithin (PC).

[0072] The soybean lecithin was detected by using a Shimadzu liquid chromatograph LC-20A, as shown in Figure 1 Figure 1 The liquid chromatogram of the soybean lecithin in Example 1 is shown in Figure 1 It can be seen that the purity of the soybean lecithin is 70.32%.

[0073] Example 2

[0074] The method for preparing the soybean lecithin in this example is basically the same as that in Example 1, except that:

[0075] (1) 1 g of soybean phospholipid and 10 g of acetone are replaced by 1 g of soybean phospholipid and 15 g of acetone.

[0076] The method in this example finally obtains 0.3 g of soybean lecithin (PC) in mass.

[0077] Figure 2 The liquid chromatogram of the soybean lecithin in Example 2 is shown in Figure 2 It can be seen that the purity of the soybean lecithin (PC purity) is 70%.

[0078] Example 3

[0079] The method for preparing the soybean lecithin in this example is basically the same as that in Example 1, except that:

[0080] (1) 1 g of soybean phospholipid and 10 g of acetone are replaced by 1 g of soybean phospholipid and 20 g of acetone.

[0081] The method in this example finally obtains 0.23 g of soybean lecithin (PC) in mass.

[0082] Figure 3 The liquid chromatogram of the soybean lecithin in Example 3 is shown in Figure 3 It can be seen that the purity of the soybean lecithin (PC purity) is 68.44%.

[0083] Example 4

[0084] The method for preparing the soybean lecithin in this example is basically the same as that in Example 1, except that:

[0085] (1) 1 g of soybean phospholipid and 10 g of acetone are replaced by 1 g of soybean phospholipid and 1 g of acetone.

[0086] The method in this example finally obtains 0.28 g of soybean lecithin (PC) in mass.

[0087] Figure 4 The liquid chromatogram of the soybean lecithin in Example 4 is shown in Figure 4 ​It can be seen that the purity of soybean lecithin (PC purity) is 65.66%.

[0088] Example 5

[0089] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0090] (2) Replace the crude extract of solid soybean lecithin with ethanol at a mass ratio of 1:10 with a mass ratio of 1:20.

[0091] Using the method of this embodiment, 0.22 g of soybean lecithin (PC) can be obtained.

[0092] Figure 5 This is a liquid chromatogram of soybean lecithin from Example 5. Figure 5 It can be seen that the purity of soybean lecithin (PC purity) is 67.91%.

[0093] Example 6

[0094] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0095] (2) Replace the crude extract of solid soybean lecithin with ethanol at a mass ratio of 1:10 with the crude extract of solid soybean lecithin with ethanol at a mass ratio of 1:1.

[0096] Using the method of this embodiment, 0.19 g of soybean lecithin (PC) can be obtained.

[0097] Figure 6 This is a liquid chromatogram of soybean lecithin from Example 6. Figure 6 It can be seen that the purity of soybean lecithin (PC purity) is 68.21%.

[0098] Comparative Example 1 (using only phospholipase A2)

[0099] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0100] (3) After mixing phospholipase A2 with the concentrate in (2) at a mass ratio of 1:100, the mixture was enzymatically hydrolyzed at 45°C for 60 min to obtain soybean lecithin concentrate.

[0101] Using the method in this comparative example, 0.25 g of soybean lecithin (PC) can be obtained.

[0102] Figure 7 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 1. Figure 7 It can be seen that the purity of soybean lecithin (PC purity) is 50.21%.

[0103] Comparative Example 2 (use of only lipase)

[0104] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0105] (3) The lipase and the concentrated solution in (2) were mixed at a mass ratio of 1:100, and then enzymolysis was performed at 45°C for 60 min to obtain a soybean lecithin concentrate.

[0106] Using the method of this comparative example, 0.19 g of soybean lecithin (PC) can finally be obtained.

[0107] Figure 8 The liquid chromatogram of the soybean lecithin in Comparative Example 2 is shown in FIG. 2. As can be seen from FIG. 2, Figure 8 the purity of the soybean lecithin (PC purity) was 44.69%.

[0108] Comparative Example 3 (use of only protease)

[0109] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0110] (3) The protease and the concentrated solution in (2) were mixed at a mass ratio of 1:100, and then enzymolysis was performed at 45°C for 60 min to obtain a soybean lecithin concentrate.

[0111] Using the method of this comparative example, 0.17 g of soybean lecithin (PC) can finally be obtained.

[0112] Figure 9 The liquid chromatogram of the soybean lecithin in Comparative Example 3 is shown in FIG. 3. As can be seen from FIG. 3, Figure 9 the purity of the soybean lecithin (PC purity) was 46.36%.

[0113] Comparative Example 4 (use of a complex enzyme composed of phospholipase A2 and lipase)

[0114] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0115] (3) The complex enzyme and the concentrated solution in (2) were mixed at a mass ratio of 1:100, and then enzymolysis was performed at 45°C for 60 min to obtain a soybean lecithin concentrate; the complex enzyme was composed of phospholipase A2 and lipase at a mass ratio of 1:3.

[0116] Using the method of this comparative example, 0.23 g of soybean lecithin (PC) can finally be obtained.

[0117] Figure 10 The liquid chromatogram of the soybean lecithin in Comparative Example 4 is shown in FIG. 4. As can be seen from FIG. 4,Figure 10 It can be seen that the purity of soybean lecithin (PC purity) is 56.87%.

[0118] Comparative Example 5 (using a complex enzyme composed of phospholipase A2 and protease)

[0119] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0120] (3) Mix the compound enzyme with the concentrate in (2) at a mass ratio of 1:100 and then enzymatically hydrolyze it at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme consists of phospholipase A2 and protease at a mass ratio of 1:2.

[0121] Using the method in this comparative example, 0.21 g of soybean lecithin (PC) can be obtained.

[0122] Figure 11 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 5. Figure 11 It can be seen that the purity of soybean lecithin (PC purity) is 59.13%.

[0123] Comparative Example 6 (using a complex enzyme composed of lipase and protease)

[0124] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0125] (3) Mix the compound enzyme with the concentrate in (2) at a mass ratio of 1:100 and then enzymatically hydrolyze it at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme is composed of lipase and protease at a mass ratio of 3:2.

[0126] Using the method in this comparative example, 0.18 g of soybean lecithin (PC) can be obtained.

[0127] Figure 12 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 6. Figure 12 It can be seen that the purity of soybean lecithin (PC purity) is 51.39%.

[0128] Comparative Example 7 (without acetone)

[0129] This comparative example provides a method for producing soybean lecithin, comprising:

[0130] (1) Mix 1g of soybean lecithin with 10g of ethanol to obtain a mixture. Stir the mixture at 200r / min for 25min. After solid-liquid separation, obtain a crude soybean lecithin extract. Concentrate the crude soybean lecithin extract under vacuum until the weight percentage of solute is 50% to obtain a concentrated solution.

[0131] (2) The complex enzyme is mixed with the concentrated solution according to a mass ratio of 1:100, and then enzymolysis is carried out at 45°C for 60 min to obtain the soybean lecithin concentrate; the complex enzyme is composed of phospholipase A2, lipase and protease according to a mass ratio of 1:3:2;

[0132] (3) The soybean lecithin concentrate is subjected to enzyme inactivation treatment at 85°C for 10 min to obtain the enzyme-inactivated soybean lecithin concentrate; the enzyme-inactivated soybean lecithin concentrate is subjected to drying treatment at 49°C for 90 min to obtain the soybean lecithin.

[0133] The method of the present comparative example can finally obtain 0.19 g of soybean lecithin (PC).

[0134] Figure 13 The liquid chromatogram of the soybean lecithin in Comparative Example 7 is shown in FIG. 1. Figure 13 It can be seen that the purity of the soybean lecithin (PC purity) is 31.83%.

[0135] Performance test

[0136] 1. Moisture retention rate test

[0137] The soybean lecithin in Examples 1-5 and Comparative Examples 1-9 is subjected to moisture retention tests, and the test results are shown in Table 1. The specific test method is as follows: 5 g of the soybean lecithin in Examples 1-6 and Comparative Examples 1-7 is placed in a weighing bottle, and is placed in an environment with a relative humidity of 40% and 80% at room temperature for 48 h, respectively. The mass of the soybean lecithin after being placed for 48 h is weighed, and the moisture retention rate is calculated according to the following formula:

[0138] Moisture retention rate = (mass of soybean lecithin after being placed for 48 h / mass of lecithin before being placed) x 100%.

[0139] Table 1. Test results

[0140]

[0141]

[0142] As shown in Table 1, the soybean lecithin prepared in Examples 1-6 of the present application has a high moisture retention rate, which can be as high as 91.4%, indicating that the soybean lecithin provided by the present application has excellent moisturizing effect.

[0143] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and are not intended to limit the present application; although the present application has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions recorded in the above embodiments can be modified, or some or all of the technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for extracting soybean lecithin from soybean lecithin, characterized in that, include: The first mixture of soybean lecithin and acetone was subjected to a first stirring treatment to obtain a solid crude soybean lecithin extract. The second mixture of the solid soybean lecithin crude extract and ethanol is subjected to a second stirring treatment to obtain a soybean lecithin crude extract. The soybean lecithin crude extract is then concentrated to a solid content of 45% to 55% to obtain a concentrated solution. The concentrate was enzymatically hydrolyzed using a complex enzyme to obtain a soybean lecithin concentrate, wherein the complex enzyme consisted of phospholipase A2, lipase and protease. The soybean lecithin concentrate is subjected to enzyme inactivation treatment and drying treatment to obtain the soybean lecithin.

2. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The mass ratio of soybean lecithin to acetone is 1:(1-20).

3. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The mass ratio of the solid soybean phospholipid crude extract to ethanol is 1:(1-20).

4. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, In the complex enzyme, the mass ratio of phospholipase A2, lipase and protease is 1:3:

2.

5. The method for extracting soybean lecithin from soybean phospholipids according to claim 4, characterized in that, The mass ratio of the compound enzyme to the concentrate is 1:(80-120).

6. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The concentrate is enzymatically hydrolyzed using a complex enzyme, specifically including: After mixing the compound enzyme with the concentrated solution, enzymatic hydrolysis was carried out at 40℃~50℃ for 50min~65min.

7. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The first stirring treatment has a rotation speed of 80 r / min to 120 r / min and a time of 20 min to 60 min.

8. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The second stirring treatment is performed at a speed of 150 r / min to 250 r / min for a time of 20 min to 30 min.

9. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The enzyme inactivation treatment is performed at a temperature of 80℃ to 100℃ for a time of 5 min to 15 min.

10. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The drying process is carried out at a temperature of 45℃ to 50℃ for a time of 80 min to 100 min.

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