A method for extracting soy lecithin from soy phospholipids

Abstract

This invention relates to the field of biotechnology, and more particularly to a method for extracting soybean lecithin from soybean lecithin. The method includes: subjecting a first mixture of soybean lecithin and acetone to a first stirring treatment to obtain a solid crude soybean lecithin extract; subjecting a second mixture of the solid crude soybean lecithin extract and ethanol to a second stirring treatment to obtain a crude soybean lecithin extract solution; concentrating the crude soybean lecithin extract solution to obtain a concentrated solution; enzymatically hydrolyzing the concentrated solution using a complex enzyme, the complex enzyme being composed of phospholipase A2, lipase, and protease, to obtain a concentrated soybean lecithin solution; and subjecting the concentrated soybean lecithin solution to enzyme inactivation treatment and drying treatment to obtain the soybean lecithin. This method can effectively improve the yield and purity of soybean lecithin, and the extracted soybean lecithin has a high moisture retention rate, showing broad application prospects in the food industry.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and more particularly to a method for extracting soybean lecithin from soybean phospholipids. Background Technology

[0002] Lecithin is a group of yellowish-brown oily substances found in animal and plant tissues and egg yolks. Due to its excellent properties and various physiological functions, it has received widespread attention. Soy lecithin, a natural plant-derived lecithin extracted from soybeans, mainly consists of phosphatidylcholine, phosphatidylethanolamine, and small amounts of triglycerides and fatty acids. Because of its good emulsifying, moisturizing, and antioxidant properties, it is used in the food industry as an emulsifier, preservative, and anti-caking agent.

[0003] Currently, the main methods for extracting soybean lecithin include organic solvent extraction, column chromatography, ultrasonic extraction, and supercritical fluid extraction. Organic solvent extraction is the most commonly used, but the purity of the extracted soybean lecithin is relatively low. Column chromatography yields higher purity soybean lecithin, but it suffers from complex separation processes, long extraction times, and large amounts of elution solvent, making industrialization difficult. Ultrasonic extraction utilizes the cavitation effect of ultrasound to accelerate the extraction of soybean lecithin, while supercritical fluid extraction uses supercritical fluid as a solvent. However, both methods require specialized equipment, resulting in high investment costs, complex operations, and high production costs.

[0004] Therefore, how to increase the yield and purity of soybean lecithin while reducing production costs is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] This invention provides a method for extracting soybean lecithin from soybean lecithin. This method can effectively improve the yield and purity of soybean lecithin, and the extracted soybean lecithin has a high moisturizing rate, which has broad application prospects in the food industry. The method is simple to operate, does not require the use of special extraction equipment, can effectively reduce production costs, and is suitable for widespread application.

[0006] This invention provides a method for extracting soybean lecithin from soybean phospholipids, comprising:

[0007] The first mixture of soybean lecithin and acetone was subjected to a first stirring treatment to obtain a solid crude soybean lecithin extract.

[0008] The second mixture of the solid soybean lecithin crude extract and ethanol is subjected to a second stirring treatment to obtain a soybean lecithin crude extract. The soybean lecithin crude extract is then concentrated to a solid content of 45% to 55% to obtain a concentrated solution.

[0009] The concentrate was enzymatically hydrolyzed using a complex enzyme to obtain a soybean lecithin concentrate, wherein the complex enzyme consisted of phospholipase A2, lipase and protease.

[0010] The soybean lecithin concentrate is subjected to enzyme inactivation treatment and drying treatment to obtain the soybean lecithin.

[0011] In the method for extracting soybean lecithin from soybean lecithin as described above, the mass ratio of soybean lecithin to acetone is 1:(1-20).

[0012] In the method for extracting soybean lecithin from soybean lecithin as described above, the mass ratio of the solid soybean lecithin crude extract to ethanol is 1:(1-20).

[0013] In the method for extracting soybean lecithin from soybean phospholipids as described above, the mass ratio of phospholipase A2, lipase, and protease in the complex enzyme is 1:3:2.

[0014] In the method for extracting soybean lecithin from soybean phospholipids as described above, the mass ratio of the complex enzyme to the concentrate is 1:(80-120).

[0015] The method for extracting soybean lecithin from soybean phospholipids as described above involves enzymatic hydrolysis of the concentrate using a complex enzyme, specifically including:

[0016] After mixing the compound enzyme with the concentrated solution, enzymatic hydrolysis was carried out at 40℃~50℃ for 50min~65min.

[0017] In the method for extracting soybean lecithin from soybean lecithin as described above, the first stirring treatment is performed at a speed of 80 r / min to 120 r / min for a time of 20 min to 60 min.

[0018] In the method for extracting soybean lecithin from soybean lecithin as described above, the second stirring treatment is performed at a speed of 150 r / min to 250 r / min for a time of 20 min to 30 min.

[0019] In the method for extracting soybean lecithin from soybean lecithin as described above, the enzyme inactivation treatment is carried out at a temperature of 80℃ to 100℃ for a time of 5 min to 15 min.

[0020] In the method for extracting soybean lecithin from soybean lecithin as described above, the drying process is carried out at a temperature of 45°C to 50°C for a time of 80 min to 100 min.

[0021] Compared with the prior art, the present invention has at least the following beneficial effects:

[0022] This invention provides a method for extracting soybean lecithin from soybean lecithin. First, soybean lecithin is extracted with acetone to obtain a solid crude soybean lecithin extract. Then, the solid crude soybean lecithin extract is extracted with ethanol to obtain a crude soybean lecithin extract solution. This solution is then concentrated to obtain a concentrated solution. Next, the concentrated solution is enzymatically hydrolyzed using a complex enzyme composed of phospholipase A2, lipase, and protease to obtain a concentrated soybean lecithin solution. Finally, the concentrated soybean lecithin solution is subjected to enzyme inactivation treatment and drying to obtain soybean lecithin. This method effectively improves the yield and purity of soybean lecithin, and the extracted soybean lecithin has a high moisture content, showing broad application prospects in the food industry. Furthermore, this method is simple to operate, requires no specialized extraction equipment, has low production costs, and is suitable for widespread application. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a liquid chromatogram of soybean lecithin in Example 1. The vertical axis represents the electrical signal intensity / mV, and the horizontal axis represents the retention time / min.

[0025] Figure 2 This is a liquid chromatogram of soybean lecithin in Example 2. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0026] Figure 3 This is the liquid chromatogram of soybean lecithin in Example 3. The vertical axis represents the electrical signal intensity / mV, and the horizontal axis represents the retention time / min.

[0027] Figure 4 This is the liquid chromatogram of soybean lecithin in Example 4. The vertical axis represents the electrical signal intensity / mV, and the horizontal axis represents the retention time / min.

[0028] Figure 5 This is the liquid chromatogram of soybean lecithin in Example 5. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0029] Figure 6 This is the liquid chromatogram of soybean lecithin in Example 6. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0030] Figure 7The image shows the liquid chromatogram of soybean lecithin in Comparative Example 1. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0031] Figure 8 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 2. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0032] Figure 9 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 3. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0033] Figure 10 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 4. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0034] Figure 11 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 5. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0035] Figure 12 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 6. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min.

[0036] Figure 13 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 7. The vertical axis represents the electrical signal intensity in mV, and the horizontal axis represents the retention time in min. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions in the embodiments of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0038] Unless otherwise specified, the raw materials and reagents used in the following examples are commercially available; and the processes used are conventional processes in the art.

[0039] It should be noted that the use of terms such as "first" and "second" in this invention is for distinguishing similar objects and not for describing a specific order or sequence, and therefore should not be construed as a limitation of this invention.

[0040] Furthermore, in the specification and claims, "and / or" indicates at least one of the connected objects, and the character " / " generally indicates that the preceding and following related objects are in an "or" relationship.

[0041] This invention provides a method for extracting soybean lecithin from soybean phospholipids, comprising:

[0042] The first mixture of soybean lecithin and acetone was subjected to a first stirring treatment to obtain a solid crude soybean lecithin extract.

[0043] The second mixture of solid soybean lecithin crude extract and ethanol is subjected to a second stirring treatment to obtain soybean lecithin crude extract. The soybean lecithin crude extract is then concentrated to a solid content of 45% to 55% to obtain a concentrated solution.

[0044] The concentrate was enzymatically hydrolyzed using a compound enzyme to obtain soybean lecithin concentrate, wherein the compound enzyme consisted of phospholipase A2, lipase and protease.

[0045] Soybean lecithin concentrate is obtained by inactivating enzymes and drying the concentrate.

[0046] The present invention does not impose any particular limitation on the specific source of the raw materials and reagents used in the above method, which can be purchased from commercial channels.

[0047] Specifically, this invention involves mixing soybean lecithin with acetone to obtain a first mixture. The first mixture is then subjected to a first stirring treatment to ensure thorough and uniform mixing of the soybean lecithin and acetone. After solid-liquid separation, the oil is initially removed to obtain a solid crude soybean lecithin extract. Next, the solid crude soybean lecithin extract is mixed with ethanol to obtain a second mixture. This second mixture is then subjected to a second stirring treatment to ensure thorough mixing of the solid crude soybean lecithin extract with ethanol. After solid-liquid separation, a crude soybean lecithin extract solution is obtained. This solution is then concentrated to a solid content of 45%. 55% concentration was used to obtain a concentrated solution. Concentration was used to increase the lecithin concentration, which is beneficial for subsequent enzymatic hydrolysis. Then, a complex enzyme consisting of phospholipase A2, lipase, and protease was mixed with the concentrated solution for enzymatic hydrolysis to obtain a concentrated soybean lecithin solution. Enzymatic hydrolysis can purify lecithin and improve its hydrophilicity. The concentrated soybean lecithin solution was then subjected to enzyme inactivation treatment to obtain an enzyme-inactivated concentrated soybean lecithin solution. Enzyme inactivation treatment is used to prevent deep hydrolysis of lecithin. Finally, the enzyme-inactivated concentrated soybean lecithin solution was dried to obtain soybean lecithin. The purpose of drying treatment is to prevent oxidation.

[0048] The present invention can effectively improve the extraction rate of soybean lecithin through the above method, and the extracted soybean lecithin has the advantages of high purity and high hydrophilicity; the method is simple to operate, does not require the use of special extraction equipment, and has low production cost.

[0049] In one specific embodiment, the mass ratio of the above-mentioned soybean lecithin to acetone is 1:(1-20).

[0050] When the mass ratio of soybean lecithin to acetone is within the above range, it is beneficial to improve the yield and purity of soybean lecithin.

[0051] For example, the mass ratio of soybean lecithin to acetone can be any one or any combination of two of the following: 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20.

[0052] In one specific embodiment, the mass ratio of the above-mentioned solid soybean phospholipid crude extract to ethanol is 1:(1-15).

[0053] When the mass ratio of solid soybean lecithin crude extract to ethanol is within the above range, it is beneficial to improve the yield and purity of soybean lecithin.

[0054] For example, the mass ratio of the above-mentioned solid soybean lecithin crude extract to ethanol can be any one or any combination of two of the following: 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20.

[0055] In one specific embodiment, the mass ratio of phospholipase A2, lipase and protease in the above-mentioned complex enzyme is 1:3:2.

[0056] The inventors' research shows that when the compound enzyme used is composed of phospholipase A2, lipase and protease in a mass ratio of 1:3:2, it can fully exert a synergistic effect and effectively improve the yield and purity of soybean lecithin.

[0057] In one specific embodiment, the mass ratio of the above-mentioned compound enzyme to the concentrate is 1:(80-120), preferably 1:100.

[0058] In one specific embodiment, the above-mentioned concentrate is enzymatically hydrolyzed using a complex enzyme, including:

[0059] After mixing the compound enzyme with the concentrated solution, enzymatic hydrolysis was carried out at 40℃~50℃ for 50min~65min.

[0060] In one specific embodiment, the speed of the first stirring treatment is 80 r / min to 120 r / min and the time is 20 min to 60 min.

[0061] In one specific embodiment, the second stirring treatment is performed at a speed of 150 r / min to 250 r / min for a time of 20 min to 30 min.

[0062] In one specific embodiment, the temperature of the enzyme inactivation treatment is 80℃~100℃ and the time is 5min~15min.

[0063] In one specific embodiment, the temperature of the above drying process is 45°C to 50°C and the time is 80 min to 100 min.

[0064] The present invention will be further described below through specific embodiments.

[0065] The raw materials, soybean phospholipids, were purchased from Zhongchuang Engineering Technology Co., Ltd.; acetone from Wuhan Geao Hengsheng Technology Development Co., Ltd.; ethanol from Wuhan Geao Hengsheng Technology Development Co., Ltd.; phospholipase A2 from Shanghai Kanglang Biotechnology Co., Ltd.; lipase from Wuhan Xinhua Yang Biotechnology Co., Ltd.; and protease from Wuhan Xinhua Yang Biotechnology Co., Ltd.

[0066] Example 1

[0067] This embodiment provides a method for producing soybean lecithin, including:

[0068] (1) Mix 1g of soybean lecithin with 10g of acetone to obtain a first mixture. Then, the first mixture is stirred at 100r / min for 30min. After solid-liquid separation, a solid crude soybean lecithin extract is obtained.

[0069] (2) The solid soybean lecithin crude extract in (1) is mixed with ethanol at a mass ratio of 1:10 to obtain a second mixture. The second mixture is subjected to a second stirring treatment at a speed of 200 r / min for 25 min. After solid-liquid separation, the soybean lecithin crude extract is obtained. The soybean lecithin crude extract is concentrated under vacuum to a solid content of 50% to obtain a concentrated liquid.

[0070] (3) After mixing the compound enzyme with the concentrate in (2) at a mass ratio of 1:100, the mixture was enzymatically hydrolyzed at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme was composed of phospholipase A2, lipase and protease at a mass ratio of 1:3:2.

[0071] (4) The soybean lecithin concentrate in (3) was subjected to enzyme inactivation treatment at 85°C for 10 min to obtain enzyme-inactivated soybean lecithin concentrate; finally, the enzyme-inactivated soybean lecithin concentrate was dried at 49°C for 90 min to obtain 0.31 g soybean lecithin (PC).

[0072] Soybean lecithin was detected using a Shimadzu LC-20A liquid chromatograph. Figure 1 As shown. Figure 1 This is a liquid chromatogram of soybean lecithin from Example 1. Figure 1 It can be seen that the purity of soybean lecithin is 70.32%.

[0073] Example 2

[0074] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0075] (1) Replace 1g of soybean lecithin and 10g of acetone with 1g of soybean lecithin and 15g of acetone.

[0076] Using the method of this embodiment, 0.3g of soybean lecithin (PC) was finally obtained.

[0077] Figure 2 This is a liquid chromatogram of soybean lecithin from Example 2. Figure 2 It can be seen that the purity of soybean lecithin (PC purity) is 70%.

[0078] Example 3

[0079] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0080] (1) Replace 1g of soybean lecithin and 10g of acetone with 1g of soybean lecithin and 20g of acetone.

[0081] Using the method of this embodiment, 0.23g of soybean lecithin (PC) can be obtained.

[0082] Figure 3 This is a liquid chromatogram of soybean lecithin from Example 3. Figure 3 It can be seen that the purity of soybean lecithin (PC purity) is 68.44%.

[0083] Example 4

[0084] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0085] (1) Replace 1g of soybean lecithin and 10g of acetone with 1g of soybean lecithin and 1g of acetone.

[0086] Using the method of this embodiment, 0.28g of soybean lecithin (PC) can be obtained.

[0087] Figure 4 This is a liquid chromatogram of soybean lecithin from Example 4. Figure 4It can be seen that the purity of soybean lecithin (PC purity) is 65.66%.

[0088] Example 5

[0089] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0090] (2) Replace the crude extract of solid soybean lecithin with ethanol at a mass ratio of 1:10 with a mass ratio of 1:20.

[0091] Using the method of this embodiment, 0.22 g of soybean lecithin (PC) can be obtained.

[0092] Figure 5 This is a liquid chromatogram of soybean lecithin from Example 5. Figure 5 It can be seen that the purity of soybean lecithin (PC purity) is 67.91%.

[0093] Example 6

[0094] The method for soybean lecithin provided in this embodiment is basically the same as that in Example 1, except that:

[0095] (2) Replace the crude extract of solid soybean lecithin with ethanol at a mass ratio of 1:10 with the crude extract of solid soybean lecithin with ethanol at a mass ratio of 1:1.

[0096] Using the method of this embodiment, 0.19 g of soybean lecithin (PC) can be obtained.

[0097] Figure 6 This is a liquid chromatogram of soybean lecithin from Example 6. Figure 6 It can be seen that the purity of soybean lecithin (PC purity) is 68.21%.

[0098] Comparative Example 1 (using only phospholipase A2)

[0099] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0100] (3) After mixing phospholipase A2 with the concentrate in (2) at a mass ratio of 1:100, the mixture was enzymatically hydrolyzed at 45°C for 60 min to obtain soybean lecithin concentrate.

[0101] Using the method in this comparative example, 0.25 g of soybean lecithin (PC) can be obtained.

[0102] Figure 7 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 1. Figure 7 It can be seen that the purity of soybean lecithin (PC purity) is 50.21%.

[0103] Comparative Example 2 (using only lipase)

[0104] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0105] (3) After mixing the lipase with the concentrate in (2) at a mass ratio of 1:100, the mixture was enzymatically hydrolyzed at 45°C for 60 min to obtain soybean lecithin concentrate.

[0106] Using the method of this comparative example, 0.19 g of soybean lecithin (PC) can be obtained.

[0107] Figure 8 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 2. Figure 8 It can be seen that the purity of soybean lecithin (PC purity) is 44.69%.

[0108] Comparative Example 3 (using only protease)

[0109] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0110] (3) Mix the protease with the concentrate in (2) at a mass ratio of 1:100 and then perform enzymatic hydrolysis at 45°C for 60 min to obtain soybean lecithin concentrate.

[0111] Using the method in this comparative example, 0.17 g of soybean lecithin (PC) can be obtained.

[0112] Figure 9 The image shows the liquid chromatogram of soybean lecithin in Comparative Example 3. Figure 9 It can be seen that the purity of soybean lecithin (PC purity) is 46.36%.

[0113] Comparative Example 4 (using a complex enzyme composed of phospholipase A2 and lipase)

[0114] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0115] (3) Mix the compound enzyme with the concentrate in (2) at a mass ratio of 1:100 and then enzymatically hydrolyze it at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme is composed of phospholipase A2 and lipase at a mass ratio of 1:3.

[0116] Using the method in this comparative example, 0.23 g of soybean lecithin (PC) can be obtained.

[0117] Figure 10 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 4. Figure 10 It can be seen that the purity of soybean lecithin (PC purity) is 56.87%.

[0118] Comparative Example 5 (using a complex enzyme composed of phospholipase A2 and protease)

[0119] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0120] (3) Mix the compound enzyme with the concentrate in (2) at a mass ratio of 1:100 and then enzymatically hydrolyze it at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme is composed of phospholipase A2 and protease at a mass ratio of 1:2.

[0121] Using the method in this comparative example, 0.21 g of soybean lecithin (PC) can be obtained.

[0122] Figure 11 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 5. Figure 11 It can be seen that the purity of soybean lecithin (PC purity) is 59.13%.

[0123] Comparative Example 6 (using a complex enzyme composed of lipase and protease)

[0124] The method for providing soybean lecithin in this comparative example is basically the same as that in Example 1, except that:

[0125] (3) Mix the compound enzyme with the concentrate in (2) at a mass ratio of 1:100 and then enzymatically hydrolyze it at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme is composed of lipase and protease at a mass ratio of 3:2.

[0126] Using the method in this comparative example, 0.18 g of soybean lecithin (PC) can be obtained.

[0127] Figure 12 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 6. Figure 12 It can be seen that the purity of soybean lecithin (PC purity) is 51.39%.

[0128] Comparative Example 7 (without acetone)

[0129] This comparative example provides a method for producing soybean lecithin, comprising:

[0130] (1) Mix 1g of soybean lecithin with 10g of ethanol to obtain a mixture. Stir the mixture at 200r / min for 25min. After solid-liquid separation, obtain a crude soybean lecithin extract. Concentrate the crude soybean lecithin extract under vacuum until the weight percentage of solute is 50% to obtain a concentrated solution.

[0131] (2) After mixing the compound enzyme with the concentrate at a mass ratio of 1:100, the mixture was enzymatically hydrolyzed at 45°C for 60 min to obtain soybean lecithin concentrate; the compound enzyme was composed of phospholipase A2, lipase and protease at a mass ratio of 1:3:2.

[0132] (3) The soybean lecithin concentrate was subjected to enzyme inactivation treatment at 85℃ for 10 min to obtain the enzyme-inactivated soybean lecithin concentrate; the enzyme-inactivated soybean lecithin concentrate was dried at 49℃ for 90 min to obtain soybean lecithin.

[0133] Using the method of this comparative example, 0.19 g of soybean lecithin (PC) can be obtained.

[0134] Figure 13 The image shows the liquid chromatogram of soybean lecithin from Comparative Example 7. Figure 13 It can be seen that the purity of soybean lecithin (PC purity) is 31.83%.

[0135] Performance testing

[0136] 1. Moisturizing rate test

[0137] Moisturizing properties were tested on the soybean lecithin from Examples 1-5 and Comparative Examples 1-9, and the results are shown in Table 1. The specific testing method is as follows: 5g of soybean lecithin from Examples 1-6 and Comparative Examples 1-7 were weighed and placed in weighing bottles. The bottles were then placed at room temperature in environments with relative humidity of 40% and 80% for 48 hours, respectively. The mass of the soybean lecithin after 48 hours was weighed, and the moisturizing rate was calculated using the following formula:

[0138] Moisture retention rate = (mass of soybean lecithin after 48 hours of storage / mass of lecithin before storage) × 100%.

[0139] Table 1 Test Results

[0140]

[0141]

[0142] As shown in Table 1, the soybean lecithin prepared in Examples 1-6 of the present invention has a high moisturizing rate, up to 91.4%, which indicates that the soybean lecithin provided by the present invention has an excellent moisturizing effect.

[0143] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for extracting soybean lecithin from soybean lecithin, characterized in that, include: The first mixture of soybean lecithin and acetone was subjected to a first stirring treatment to obtain a solid crude soybean lecithin extract. The second mixture of the solid soybean lecithin crude extract and ethanol is subjected to a second stirring treatment to obtain a soybean lecithin crude extract. The soybean lecithin crude extract is then concentrated to a solid content of 45% to 55% to obtain a concentrated solution. The concentrate was enzymatically hydrolyzed using a complex enzyme to obtain a soybean lecithin concentrate, wherein the complex enzyme consisted of phospholipase A2, lipase and protease. The soybean lecithin concentrate is subjected to enzyme inactivation treatment and drying treatment to obtain the soybean lecithin. The mass ratio of soybean phospholipid to acetone is 1:(1-20); the mass ratio of the crude solid soybean phospholipid extract to ethanol is 1:(1-20); and the mass ratio of phospholipase A2, lipase, and protease in the complex enzyme is 1:3:

2.

2. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The mass ratio of the compound enzyme to the concentrate is 1:(80-120).

3. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The concentrate is enzymatically hydrolyzed using a complex enzyme, specifically including: After mixing the compound enzyme with the concentrated solution, enzymatic hydrolysis was carried out at 40℃~50℃ for 50min~65min.

4. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The first stirring treatment has a rotation speed of 80 r / min to 120 r / min and a time of 20 min to 60 min.

5. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The second stirring treatment is performed at a speed of 150 r / min to 250 r / min for a time of 20 min to 30 min.

6. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The enzyme inactivation treatment is performed at a temperature of 80℃ to 100℃ for a time of 5 min to 15 min.

7. The method for extracting soybean lecithin from soybean phospholipids according to claim 1, characterized in that, The drying process is carried out at a temperature of 45℃ to 50℃ for a time of 80 min to 100 min.

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