Micro-nano bubble observation device and observation method

Abstract

The application discloses a kind of micro-nano bubble observation device and observation method, including micro-nano bubble generation module, for observing the microscope observation module of micro-nano bubble, rotatable flow channel module is installed on the microscope observation module, for the communication of micro-nano bubble generation module and rotatable flow channel is carried out synergic control module.The application is set by rotatable flow channel module, so that micro-nano bubble can be in situ dynamic observation, improve the observation precision of micro-nano bubble.

Description

Technical Field

[0001] This invention relates to the field of micro-nano bubble observation technology, specifically to a micro-nano bubble observation device and observation method. Background Technology

[0002] Micro- and nanobubbles are a class of tiny bubbles with diameters in the micrometer and nanometer ranges. Although different scholars may define their diameter range slightly differently, most generally refer to bubbles with a diameter of less than 100 μm as micro- and nanobubbles. Compared with traditional bubbles, micro- and nanobubbles have the characteristics of small volume, large specific surface area, long existence time, good mass transfer efficiency, high surface potential, strong biological activity, and the ability to generate free radicals. Among them, the hydroxyl radicals generated by micro- and nanobubbles have strong oxidizing properties and are non-selective in the removal of pollutants, effectively removing various organic pollutants, recalcitrant pollutants, and pathogenic microorganisms from water.

[0003] In recent years, the actual state and stability mechanism of micro and nanobubbles at the microscale have become a new research hotspot, such as the behavior of nanobubbles in liquid environments and their survival characteristics. Therefore, a method for directly observing the state of nanobubbles in liquid environments is urgently needed.

[0004] Current methods for observing micro and nanobubbles mainly include scanning electron microscopy, atomic force microscopy, and in-situ observation techniques. Each of these methods has its own limitations, as detailed below:

[0005] 1. Non-in-situ and destructive nature of traditional observation methods: Scanning electron microscopy (SEM) in existing technologies may introduce new bubbles during the freezing process, while atomic force microscopy (AFM) requires physical contact with bubbles. Neither of these methods can achieve in-situ non-destructive observation of bubbles in the liquid environment.

[0006] 2. Inability to simultaneously measure concentration and dynamic parameters: Existing optical observation technology can only measure bubble size and cannot quantitatively analyze the dynamic relationship between concentration and its pressure and flow rate;

[0007] 3. Limited observation angle: Traditional microscope observation is limited by a fixed viewing angle, making it difficult to capture the three-dimensional shape of bubbles and unable to measure the true diameter of bubbles;

[0008] 4. Experimental costs and operational complexity: Equipment such as scanning electron microscopes are expensive and require professional personnel to operate.

[0009] To reduce experimental costs and operational difficulties, avoid the influence of the measurement process on the bubbles, and improve the accuracy of bubble diameter measurements, a micro / nano bubble observation device and method are needed to address the aforementioned issues. Summary of the Invention

[0010] The purpose of this invention is to provide a micro / nano bubble observation device and method to solve the problems mentioned in the background art.

[0011] To achieve the above objectives, the present invention provides a micro / nano bubble observation device, including a micro / nano bubble generating module, a microscope observation module for observing micro / nano bubbles, a rotatable flow channel module mounted on the microscope observation module, and a collaborative control module for connecting the micro / nano bubble generating module and the rotatable flow channel.

[0012] Optionally, the micro-nano bubble generating module includes a water storage tank and a micro-nano bubble generator, with an inlet pipe and an outlet pipe installed on the micro-nano bubble generator. Both the inlet and outlet pipes are equipped with control valves and are connected to the water storage tank. The micro-nano bubble generator draws water from the water storage tank through the inlet pipe and mixes it with gas, and then re-exports the mixture into the water storage tank through the outlet pipe.

[0013] Optionally, the microscope observation module includes an optical microscope, a CCD camera mounted on top of the optical microscope, a data transmission line on the CCD camera, and a computer connected to the other end of the data transmission line; wherein, the CCD camera is used in conjunction with the optical microscope to observe micro-nano bubbles, and the results observed by the CCD camera are transmitted to the computer via the data transmission line, and the computer analyzes the observation results.

[0014] Optionally, the rotatable flow channel module includes a rotating structure mounted on the stage of an optical microscope, an observation plate made of transparent material mounted on the rotating structure, and a micro / nano bubble flow channel set on the observation plate. The liquid inlet end of the micro / nano bubble flow channel is connected to a collaborative control module, and the liquid outlet end of the micro / nano bubble flow channel is provided with a return pipe. The other end of the return pipe is connected to a waste liquid pool. The rotating structure can drive the observation plate to rotate horizontally and vertically.

[0015] Optionally, the micro-nano bubble channels are arranged in an S-shape.

[0016] Optionally, the rotating structure is mounted on the optical microscope to drive the observation plate to rotate horizontally and vertically, and the observation plate is mounted on the rotating structure.

[0017] Optionally, the coordinated control module includes a sampling tube for connecting the water storage tank and the observation plate, a microfluidic pump installed on the sampling tube, and a dynamic pressure feedback actuator located on the sampling tube at the outlet end of the microfluidic pump.

[0018] This invention also discloses a method for observing micro-nano bubbles generated by a micro-nano bubble generation module using a micro-nano bubble observation device, comprising:

[0019] S1. Connect the observation device to the micro / nano bubble generation module;

[0020] S2. Start the micro-nano bubble generation module to generate micro-nano bubble solution 1;

[0021] S3. Micro-nano bubble solution one is adjusted and controlled by the collaborative regulation module to form micro-nano bubble solution two, and micro-nano bubble solution two is transported into the observation plate;

[0022] S4. After the micro-nano bubble solution 2 is filled into the observation plate, the observation plate is rotated by the rotating structure. At the same time, the microscope observation module takes a video of the flow of the micro-nano bubble solution 2 in the observation plate.

[0023] S5. Process the video using software to obtain the observation results;

[0024] S6. The bubble concentration and average bubble diameter in the micro-nano bubble solution II are calculated based on the observation results.

[0025] Optionally, the formula for calculating the total concentration of micro-nanobubbles in the micro-nanobubble solution is:

[0026] (1);

[0027] In equation (1), The total concentration of micro-nano bubbles in the micro-nano bubble solution II, in units of ; The magnification of the optical microscope (21); for Within a second, the number of all bubbles within the same diameter range as the specified bubble (the diameter range is 1 nm in length, and the division method is based on the rounding of the bubble diameter), in units of bubbles; The flow rate of the micro / nano bubble solution entering the observation plate, in units of ; This is the optical detection efficiency factor, with a value range of [value range missing]. ; The actual diameter of the selected bubbles in the micro / nano bubble solution is given in unit . .

[0028] Optionally, the formula for calculating the concentration of bubbles in the micro / nano bubble solution II that are within the same diameter range as the specified bubbles is:

[0029] (2);

[0030] In equation (2), The concentration of bubbles in the nanobubble solution II that are within the same diameter range as the specified bubbles, in units of... ; The magnification of the optical microscope (21); for Within a second, the number of all bubbles within the same diameter range as the specified bubble (the diameter range is 1 nm in length, and the division method is based on the rounding of the bubble diameter), in units of bubbles; The flow rate of the micro / nano bubble solution entering the observation plate, in units of ; This is the optical detection efficiency factor, with a value range of [value range missing]. .

[0031] Optionally, in step S3, the collaborative control module adjusts the pressure and flow rate of the micro / nano bubble solution to make the pressure reach a certain value. Traffic is Micro-nano bubble solution enters the observation plate;

[0032] In step S4, the microscope observation module observes the micro / nano bubble solution II within the observation plate for a duration of [duration missing]. , and Within a given time period, the angle by which the rotating structure causes the observation plate to rotate laterally is... The angle of vertical rotation is .

[0033] Optionally, the observed video is used to establish a three-dimensional coordinate system with the position of the selected bubble at 0 seconds as the origin, using ImageJ's TrackMate property. axis, shaft and The axis, analysis yielded the selected bubble in along the time Displacement components in the axial direction Select bubbles along the time Displacement components in the axial direction Select bubbles along the time Displacement components in the axial direction .

[0034] Optionally, the formula for calculating the actual diameter of a certain micro-nano bubble in the micro-nano bubble solution is as follows:

[0035] (3);

[0036] In equation (3), The actual diameter of the selected bubbles in the micro / nano bubble solution is given in unit . ; The Boltzmann constant for the micro / nano bubble solution is given by a value of [value missing]. ; The temperature of the micro / nano bubble solution is given in units of . ; The viscosity of the micro / nano bubble solution is given by a value of [value to be filled in]. ; To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... .

[0037] Optionally, the formula for calculating the diffusion coefficient of bubbles in the micro / nano bubble solution is selected as follows:

[0038] (4);

[0039] In equation (4), To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... ; For the selected bubbles The actual displacement within the observation plate relative to the origin at 0 seconds within a given time interval, in units of ; This refers to the time taken by the CCD camera in the microscope observation module, in seconds.

[0040] Optionally, the selected bubble is in The formula for calculating the actual displacement distance moved on the observation board within a second is:

[0041] (5);

[0042] In equation (5), For the selected bubbles The actual displacement of the movement on the observation board relative to the origin at 0 seconds within a given time interval, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of .

[0043] Optionally, select the bubble in Within seconds along The formula for calculating the displacement components in the axial direction is:

[0044] (6);

[0045] In equation (6), a three-dimensional coordinate system is established with the position of the selected bubble at 0 seconds as the origin. axis, shaft and axis; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera in the microscope observation module on a two-dimensional plane Observations along the axial direction, in units of ; For the CCD camera in the microscope observation module on a two-dimensional plane Observations along the axial direction, in units of ; The angle of horizontal rotation of the observation plate, in units of .

[0046] Optionally, select the bubble in Within seconds along The formula for calculating the displacement components in the axial direction is:

[0047] (7);

[0048] In equation (7), the position of the selected bubble at 0 seconds is used as the origin of the coordinate system to establish the x-axis, y-axis and z-axis of the three-dimensional coordinate system; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera in the microscope observation module on a two-dimensional plane Observations along the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; The angle of vertical rotation of the observation plate, in units of... ; For the CCD camera in the microscope observation module on a two-dimensional plane Observations along the axial direction, in units of ; The angle of horizontal rotation of the observation plate, in units of .

[0049] Optionally, select the bubble in Within seconds along The formula for calculating the displacement components in the axial direction is:

[0050] (8);

[0051] In equation (8), a three-dimensional coordinate system with the x-axis, y-axis and z-axis is established with the selected position of the bubble at 0 seconds as the origin; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; Reference grid offset, in units of ; This refers to the magnification of the optical microscope in the microscope observation module. The angle of vertical rotation of the observation plate, in units of... .

[0052] Compared with the prior art, the present invention provides a micro / nano bubble observation device and method, which has the following beneficial effects:

[0053] This invention, through the setting of a microscope observation module and in conjunction with a rotatable flow channel module, enables in-situ dynamic observation of the entire micro-nano bubble, thereby improving the observation accuracy of micro-nano bubbles;

[0054] This invention, through the setting of a synergistic control module, can regulate the pressure of micro-nano bubble solution. Combined with the observation of the microscope module, it can quantify the relationship between pressure and micro-nano bubble concentration, providing key parameters for studying the stability and mass transfer efficiency of micro-nano bubbles.

[0055] This invention, through the setting of a rotatable flow channel module, allows the observation plate to rotate at multiple angles. By observing micro- and nano-bubbles from multiple angles, it reduces the error of two-dimensional projection of micro- and nano-bubbles, displays the true three-dimensional morphology of micro- and nano-bubbles, and improves the accuracy of measurement.

[0056] This invention uses a CCD camera in conjunction with an optical microscope to observe micro- and nano-bubbles, reducing equipment costs and allowing for experimental operation without special training. Attached Figure Description

[0057] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0058] Figure 2 This is a schematic diagram of the rotating structure and observation plate of the present invention.

[0059] Figure 3 This is a cross-sectional view of the rotating structure of the present invention.

[0060] Figure 4 This is a schematic diagram showing the observation plate after the rotating structure of the present invention drives it to rotate horizontally and vertically.

[0061] Figure 5 This is a cross-sectional view of the observation plate of the present invention.

[0062] Figure 6 This is a schematic diagram showing the position of the bubble in the initial position of the observation plate of this invention.

[0063] Figure 7 This is a schematic diagram of the final position of the bubble after the observation plate of the present invention has rotated for t seconds.

[0064] Figure 8 This is the present invention. Figure 7 A magnified view of a portion of point A in the middle.

[0065] Figure 9 This is the present invention. Figure 7 Cross-sectional view of the observation plate BB.

[0066] Figure 10 This is a bubble size distribution diagram obtained by the present invention at 0.1 MPa.

[0067] Figure 11 This is a graph showing the changes in bubble concentration and average diameter with pressure according to the present invention.

[0068] The diagram is labeled as follows: 1. Micro / nano bubble generating module; 11. Water storage tank; 12. Micro / nano bubble generator; 13. Inlet pipe; 14. Outlet pipe; 15. Control valve; 2. Microscope observation module; 21. Optical microscope; 22. CCD camera; 23. Data transmission line; 24. Computer; 3. Rotatable flow channel module; 31. Rotating structure; 32. Observation plate; 33. Micro / nano bubble flow channel; 34. Return pipe; 35. Waste liquid tank; 4. Collaborative control module; 41. Sampling tube; 42. Microfluidic pump; 43. Dynamic pressure feedback actuator. Detailed Implementation

[0069] The invention will now be described in detail with reference to the accompanying drawings and specific embodiments. Many specific details are set forth in the following description to provide a thorough understanding of the invention. However, the invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0070] The micro-nano bubble observation device and its observation method disclosed in this application can be applied to situations such as pressure-controlled micro-nano bubble volume and concentration analysis and testing, and can also be used in other similar application scenarios. The following is a detailed description of a micro-nano bubble observation device.

[0071] See appendix Figure 1 — Figure 5 The diagram illustrates a micro / nano bubble observation device according to this application. The device includes a microscope observation module 2, a rotatable flow channel module 3, and a collaborative control module 4. The micro / nano bubble generation module 1 cyclically generates micro / nano bubbles. The microscope observation module 2 is used to observe micro / nano bubbles in a micro / nano bubble solution. The rotatable flow channel module 3 provides a flow channel for the micro / nano bubble solution and allows for horizontal and vertical rotation of the flow channel. The collaborative control module 4 is used to draw and transport the micro / nano bubble solution into the rotatable flow channel module 3, and to regulate and monitor the pressure of the micro / nano bubble solution.

[0072] See appendix Figure 1 — Figure 5 As shown, in this invention, the micro-nano bubble generating module 1 includes a water storage tank 11 and a micro-nano bubble generator 12, with an inlet pipe 13 and an outlet pipe 14 disposed on the micro-nano bubble generator 12; wherein, one end of the inlet pipe 13 is disposed at the water inlet end of the micro-nano bubble generator 12, and one end of the outlet pipe 14 is disposed at the water outlet end of the micro-nano bubble generator 12, and the other ends of the inlet pipe 13 and the outlet pipe 14 are both connected to the water storage tank 11.

[0073] The working process of the micro-nano bubble generating module 1 is as follows: the micro-nano bubble generator 12 draws the solution in the water storage tank 11 into the micro-nano bubble generator 12 through the water inlet pipe 13, generates micro-nano bubble solution in the micro-nano bubble generator 12, and then discharges the micro-nano bubble solution through the water outlet pipe 14 and re-enters the water storage tank 11.

[0074] See appendix Figure 1 As shown, in this invention, the microscope observation module 2 includes an optical microscope 21, a CCD camera 22 mounted on top of the optical microscope 21, a data transmission line 23 mounted on the CCD camera 22, and a computer 24 connected to the other end of the data transmission line 23.

[0075] This invention uses an optical microscope 21 to magnify the micro-nano bubble solution located in the micro-nano bubble channel 33, ensuring that the CCD camera 22 can capture images of the micro-nano bubble solution within the channel 33. The CCD camera 22 is configured to capture images of the micro-nano bubble solution. A data transmission line 23 is used to transmit the video captured by the CCD camera 22 to a computer 24. The computer 24, in conjunction with ImageJ+TrackMate software, analyzes the micro-nano bubbles in the video to obtain data such as the size and movement distance of each micro-nano bubble.

[0076] See appendix Figure 1 — Figure 5 As shown, in this invention, the rotatable flow channel module 3 is mounted on the rotating structure 31 on the stage of the optical microscope 21. An observation plate 32 made of transparent material is mounted on the rotating structure 31. A micro / nano bubble flow channel 33 is disposed on the observation plate 32. The inlet end of the micro / nano bubble flow channel 33 is connected to the sampling tube 41, and the outlet end of the micro / nano bubble flow channel 33 is provided with a return pipe 34. The other end of the return pipe 34 is connected to a waste liquid pool 35. It should be noted that the rotating structure 31 is existing technology and can be a piezoelectric ceramic rotating stage with multi-angle rotation, or a BW-WMG3T electric multi-dimensional rotating stage, or other existing dual-axis or multi-axis rotating stages capable of horizontal or vertical rotation. Therefore, the rotating structure 31 will not be described in detail.

[0077] The present invention uses a rotating structure 31 to drive the observation plate 32 to rotate and move, so that the CCD camera 22 can observe micro- and nano-bubbles; the observation plate 32 is equipped with micro- and nano-bubble channels 33, which allow the micro- and nano-bubble solution to flow; and the return pipe 34 is used to guide the micro- and nano-bubble solution flowing out of the micro- and nano-bubble channels 33 and transport it to the waste liquid pool 35, which, together with the waste liquid pool 35, prevents the micro- and nano-bubble solution after observation from affecting the observation structure.

[0078] See appendix Figure 1 As shown, in this invention, the collaborative control module 4 includes a sampling tube 41 for connecting the water storage tank 11 and the observation plate 32, a microfluidic pump 42 installed on the sampling tube 41, and a dynamic pressure feedback actuator 43 set on the sampling tube 41. The dynamic pressure feedback actuator 43 is located at the outlet end of the microfluidic pump 42.

[0079] This invention utilizes a sampling tube 41, in conjunction with a microfluidic pump 42, to draw and transport the micro / nano bubble solution from the water storage tank 11 into the micro / nano bubble flow channel 33. A dynamic pressure feedback actuator 43 is used to regulate and monitor the pressure of the solution entering the micro / nano bubble flow channel 33. It should be specifically noted that the inlet pipe 13, outlet pipe 14, return pipe 34, and sampling tube 41 are all flexible tubes.

[0080] See appendix Figure 1 As shown in Figure 9, the working process of this observation device is as follows:

[0081] First, micro-nano bubbles are generated in the water storage tank 11 using a micro-nano bubble generator 12. The resulting bubble particle size generally ranges from [missing value]. The concentration of bubbles produced is generally between [a certain range]. Under the control of the microfluidic pump 42, the micro-nano bubble solution in the water storage tank 11 is pumped into the micro-nano bubble channel 33. The pressure of the micro-nano bubble solution is adjusted by the dynamic pressure feedback actuator 43. The morphology and distribution of the bubbles are observed by the optical microscope 21. The angle of observation of the micro-nano bubble solution is changed by rotating the structure 31. By using an appropriate magnification and cooperating with the transmission light source to illuminate the micro-nano bubble solution, the image is ensured to be clear. The CCD camera 22 installed on the optical microscope 21 runs at a speed of 30 frames per second and can capture video files of the movement of micro-nano bubbles in the water. The size, morphology and distribution information of micro-nano bubbles are observed in real time, and the identity of bubbles under different rotation angles is verified. The micro-nano bubble image / video at a certain angle is identified by the image processing software ImageJ+TrackMate, and the displacement of each micro-nano bubble along the three-dimensional coordinate system x-axis, y-axis and z-axis and the diameter of each micro-nano bubble are obtained, thereby calculating the micro-nano bubble concentration and the average diameter of the micro-nano bubbles.

[0082] This invention also provides an observation method based on a micro / nano bubble observation device. It includes:

[0083] S1. Connect the observation device to the micro / nano bubble generating module 1;

[0084] S2. Start the micro-nano bubble generation module 1 to generate micro-nano bubble solution 1;

[0085] S3. Micro-nano bubble solution one is adjusted and controlled by the collaborative regulation module 4 to form micro-nano bubble solution two, and micro-nano bubble solution two is transported into the observation plate 32;

[0086] S4. After the micro-nano bubble solution 2 fills the observation plate 32, the observation plate 32 is rotated by the rotating structure 31. At the same time, the microscope observation module 2 takes a video of the flow of the micro-nano bubble solution 2 in the observation plate 32.

[0087] S5. Process the video using image processing software ImageJ+TrackMate to obtain the observation results;

[0088] S6. The concentration of micro-nano bubbles and the average diameter of micro-nano bubbles in the micro-nano bubble solution were calculated based on the observation results.

[0089] Specifically:

[0090] In step S6, the formula for calculating the concentration of micro-nanobubbles in the micro-nanobubble solution is:

[0091] (1);

[0092] In equation (1), The bubble concentration in the micro / nano bubble solution is given in units of... ; This refers to the magnification of the optical microscope 21. for Within a second, the number of all bubbles within the same diameter range as the specified bubble (the diameter range is 1 nm in length, and the division method is based on the rounding of the bubble diameter), in units of bubbles; The flow rate of the micro / nano bubble solution entering the observation plate 32 is expressed in units of... ; This is the optical detection efficiency factor, with a value range of [value range missing]. ; The actual diameter of the selected bubbles in the micro / nano bubble solution is given in unit . .

[0093] In the above formula, the formula for calculating the concentration of bubbles in the micro / nano bubble solution II that are within the same diameter range as the specified bubbles is:

[0094] (2);

[0095] In equation (2), The concentration of bubbles in the nanobubble solution II that are within the same diameter range as the specified bubbles, in units of... ; for Within a second, the number of all bubbles within the same diameter range as the specified bubble (the diameter range is 1 nm in length, and the division method is based on the rounding of the bubble diameter), in units of bubbles; The flow rate of the micro / nano bubble solution entering the observation plate 32 is expressed in units of... ; This is the optical detection efficiency factor, with a value range of [value range missing]. .

[0096] In equation (1) , This is determined through the collaborative control module 4, specifically the pressure exerted by the collaborative control module 4 on the micro / nano bubble solution. and traffic Adjust the pressure to make it... Traffic is The micro-nano bubble solution enters the observation plate 32.

[0097] In equation (1) , and This is determined by the microscope observation module 2 and the rotatable flow channel module 3, specifically, the duration for which the microscope observation module 2 observes the micro / nano bubble solution 2 within the observation plate 32 is [duration missing]. , and Within a given time period, the rotating structure 31 causes the observation plate 32 to rotate laterally by an angle of _____. The angle of vertical rotation is The rotation speed of the rotating structure 31 is set manually, and the lateral rotation angle is... and vertical rotation angle Automatically recorded by the rotating structure 31, simply put, by assuming a set rotation angle, the position is determined. Horizontal rotation angle within seconds and vertical rotation angle The specific value.

[0098] The formula for calculating the diffusion coefficient of selected bubbles in micro / nano bubble solution II is as follows:

[0099] (4);

[0100] In equation (4), To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... ; For the selected bubbles The actual displacement of the movement on observation board 32 relative to the origin at 0 seconds within a time interval, in units of ; The time taken by the CCD camera 22 in the microscope observation module 2 is in seconds.

[0101] Selected bubbles in The formula for calculating the actual displacement distance moved on observation plate 32 within a second is:

[0102] (5);

[0103] In equation (5), For the selected bubbles The actual displacement of the movement on observation board 32 relative to the origin at 0 seconds within a time interval, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of .

[0104] Selected bubble Within seconds along The formula for calculating the displacement components in the axial direction is:

[0105] (6);

[0106] In equation (6), a three-dimensional coordinate system is established with the position of the selected bubble at 0 seconds as the origin. axis, shaft and axis; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; The angle of horizontal rotation of the observation plate 32 is expressed in units of... .

[0107] Selected bubble Within seconds along The formula for calculating the displacement components in the axial direction is:

[0108] (7);

[0109] In equation (7), the position of the selected bubble at 0 seconds is used as the origin of the coordinate system to establish the x-axis, y-axis and z-axis of the three-dimensional coordinate system; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; The angle of vertical rotation of the observation plate is 32, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; The angle of horizontal rotation of the observation plate 32 is expressed in units of... .

[0110] Selected bubble Within seconds along The formula for calculating the displacement components in the axial direction is:

[0111] (8);

[0112] In equation (8), a three-dimensional coordinate system with the x-axis, y-axis and z-axis is established with the selected position of the bubble at 0 seconds as the origin; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; Reference grid offset, in units of ; The magnification of the optical microscope 21 in the microscope observation module 2; The angle of vertical rotation of the observation plate is 32, in units of .

[0113] Application examples

[0114] Set the magnification of the optical microscope Video recording time Horizontal rotation angle of observation plate 32 The vertical rotation angle of observation plate 32 Sample flow rate after passing through the coordinated control module 4 The gas source is air, and the temperature of the micro / nano bubble solution is... ,pressure Gas solubility coefficient In the video, the smallest diameter of the micro-nano bubble in the identified micro-nano bubble solution is [missing information]. The largest diameter of the micro-nano bubbles is The total number of valid bubbles identified in the video. The observed displacement of micro-nano bubbles with a certain particle size was identified as follows: , , Particle size distribution curve as shown Figure 10 As shown;

[0115] The particle size of micro- and nano-bubbles follows a log-normal distribution, with a mean of Standard deviation Particle size range is Total number of effective bubbles Under a normal distribution, the distribution of bubbles in each particle size range is as follows:

[0116] : accounting for approximately 15.1%, corresponding to indivual;

[0117] : accounting for approximately 19.7%, corresponding to indivual;

[0118] : accounting for approximately 21.0%, corresponding to indivual;

[0119] : accounting for approximately 19.0%, corresponding to indivual;

[0120] : accounting for approximately 14.0%, corresponding to indivual;

[0121] : accounting for approximately 7.0%, corresponding to indivual;

[0122] : accounting for approximately 3.0%, corresponding to indivual;

[0123] : accounting for approximately 1.0%, corresponding to indivual;

[0124] : accounting for approximately 0.3%, corresponding to indivual;

[0125] The percentage is approximately 0.1%, corresponding to indivual.

[0126] Calculate the actual displacement of a selected bubble in each direction using the formula:

[0127] The bubble is Within seconds along The formula for calculating the displacement components in the axial direction is:

[0128] (6);

[0129] In equation (6), a three-dimensional coordinate system is established with the position of the selected bubble at 0 seconds as the origin. axis, shaft and axis; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; The angle of horizontal rotation of the observation plate 32 is expressed in units of... ;Calculation obtained .

[0130] The bubble is Within seconds along The formula for calculating the displacement components in the axial direction is:

[0131] (8);

[0132] In equation (8), a three-dimensional coordinate system with the x-axis, y-axis and z-axis is established with the selected position of the bubble at 0 seconds as the origin; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; Reference grid offset, in units of ; The magnification of the optical microscope 21 in the microscope observation module 2; The angle of vertical rotation of the observation plate is 32, in units of ;Calculation obtained .

[0133] The bubble is Within seconds along The formula for calculating the displacement components in the axial direction is:

[0134] (7);

[0135] In equation (7), the position of the selected bubble at 0 seconds is used as the origin of the coordinate system to establish the x-axis, y-axis and z-axis of the three-dimensional coordinate system; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; The angle of vertical rotation of the observation plate is 32, in units of ; For the CCD camera 22 in microscope observation module 2 on a two-dimensional plane Observations along the axial direction, in units of ; The angle of horizontal rotation of the observation plate 32 is expressed in units of... ;Calculation obtained .

[0136] The bubble is The actual displacement moved on observation plate 32 within seconds:

[0137] (5);

[0138] In equation (5), For the selected bubbles The actual displacement of the movement on observation board 32 relative to the origin at 0 seconds within a time interval, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ;Calculation obtained .

[0139] The diffusion coefficient of this bubble in the micro / nano bubble solution is:

[0140] (4);

[0141] In equation (4), To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... ; For the selected bubbles The actual displacement of the observation plate within 32 seconds relative to the origin at 0 seconds, in units of ; The time taken by the CCD camera 22 in microscope observation module 2, in seconds; calculated as follows .

[0142] The actual diameter of the bubble is:

[0143] (3);

[0144] In equation (3), This is the actual diameter of the bubble, in units of... ; The Boltzmann constant for the micro / nano bubble solution is given by a value of [value missing]. ; The temperature of the micro / nano bubble solution is given in units of . ; The viscosity of the micro / nano bubble solution is given by a value of [value to be filled in]. ; To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... ;Calculation obtained ;

[0145] The concentration of bubbles within the same diameter range as this bubble is:

[0146] (2);

[0147] In equation (2), The concentration of bubbles in the nanobubble solution II that are within the same diameter range as the specified bubbles, in units of... ; This refers to the magnification of the optical microscope 21. for Within a second, the number of all bubbles within the same diameter range as the specified bubble (the diameter range is 1 nm in length, and the division method is based on the rounding of the bubble diameter), in units of bubbles; The flow rate of the micro / nano bubble solution entering the observation plate 32 is expressed in units of... ; The optical detection efficiency factor is set to 1.0 based on the reflection suppression algorithm; the calculated value is... .

[0148] The bubble concentration in the remaining intervals is calculated in the same manner, specifically as follows:

[0149] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0150] ;

[0151] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0152] ;

[0153] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0154] ;

[0155] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0156] ;

[0157] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0158] ;

[0159] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0160] ;

[0161] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0162] ;

[0163] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0164] ;

[0165] When the bubble diameter is Within the specified interval, the bubble concentration in the nanobubble solution 2 was calculated. for:

[0166] ;

[0167] Table 1 shows the data on bubble concentration, average diameter, and main peak position as a function of pressure.

[0168] Table 1. Effects of pressure changes on bubble concentration, average diameter, and main peak position.

[0169]

[0170] The curves showing the changes in bubble concentration and average diameter with pressure are as follows: Figure 11 As shown:

[0171] The relationship between bubble concentration and pressure is as follows:

[0172] ;

[0173] The relationship between the average diameter and pressure is as follows:

[0174] ;

[0175] Table 2 shows a comparison of the measurement results of this device with those of the widely recognized NTA (nanoparticle tracking analysis) technology under the same conditions under standard conditions.

[0176] Table 2 Comparison of measurement results from this device and NTA technology.

[0177]

[0178] The above embodiments are illustrative of this application and are not intended to limit this application. Any simple modifications to this application are within the protection scope of this application.

Claims

1. A micro / nano bubble observation device, wherein the observation device is connected to a micro / nano bubble generating module (1), characterized in that, It includes a microscope observation module (2) connected to the micro-nano bubble generating module (1), a rotatable flow channel module (3) installed in the observation area of ​​the microscope observation module (2), and a collaborative control module (4) connecting the micro-nano bubble generating module (1) and the rotatable flow channel module (3). The collaborative control module (4) is used to regulate and control the pressure and flow rate of the micro-nano bubble solution entering the observation plate (32). The rotatable flow channel module (3) includes an observation plate (32) located in the observation area of ​​the microscope observation module (2) and a rotating structure (31) for driving the observation plate (32) to rotate horizontally and vertically. The micro-nano bubble generating module (1) generates micro-nano bubbles, and the collaborative control module (4) transports the generated micro-nano bubble solution into the observation plate (32). After the micro-nano bubble solution fills the observation plate (32), the rotating structure (31) drives the observation plate (32) to rotate, and at the same time, the microscope observation module (2) observes the micro-nano bubble solution flowing in the observation plate (32).

2. The micro / nano bubble observation device according to claim 1, characterized in that, The micro-nano bubble generating module (1) includes a water storage tank (11) and a micro-nano bubble generator (12). The micro-nano bubble generator (12) has an inlet pipe (13) and an outlet pipe (14). Both the outlet pipe (14) and the inlet pipe (13) are equipped with control valves (15). Both the inlet pipe (13) and the outlet pipe (14) are connected to the water storage tank (11). The micro-nano bubble generator (12) generates micro-nano bubbles in the water storage tank (11).

3. The micro / nano bubble observation device according to claim 1, characterized in that, The microscope observation module (2) includes an optical microscope (21), a CCD camera (22) mounted on top of the optical microscope (21), a data transmission line (23) set on the CCD camera (22), and a computer (24) connected to the other end of the data transmission line (23).

4. The micro / nano bubble observation device according to claim 1, characterized in that, The rotatable flow channel module (3) also includes a micro-nano bubble flow channel (33) set on the observation plate (32). The liquid inlet end of the micro-nano bubble flow channel (33) is connected to the collaborative control module (4). The liquid outlet end of the micro-nano bubble flow channel (33) is provided with a return pipe (34). The other end of the return pipe (34) is connected to a waste liquid pool (35).

5. The micro / nano bubble observation device according to claim 4, characterized in that, The micro-nano bubble flow channel (33) is S-shaped; The rotating structure (31) is mounted on the optical microscope (21) and drives the observation plate (32) to rotate horizontally and vertically. The observation plate (32) is mounted on the rotating structure (31).

6. The micro / nano bubble observation device according to claim 1, characterized in that, The coordinated control module (4) includes a sampling tube (41) for connecting the water storage tank (11) and the observation plate (32), a microfluidic pump (42) installed on the sampling tube (41), and a dynamic pressure feedback actuator (43) set on the sampling tube (41), which is located at the outlet end of the microfluidic pump (42).

7. A method for observing micro-nano bubbles using the micro-nano bubble observation device according to any one of claims 1-6, characterized in that, include: S1. Connect the observation device to the micro-nano bubble generation module (1); S2. Start the micro-nano bubble generation module (1) to generate micro-nano bubble solution 1; S3. After the micro-nano bubble solution one is regulated and controlled by the collaborative regulation module (4), it forms micro-nano bubble solution two, and then the micro-nano bubble solution two is transported into the observation plate (32); S4. After the micro-nano bubble solution 2 fills the observation plate (32), the observation plate (32) is rotated by the rotating structure (31). At the same time, the microscope observation module (2) takes a video of the flow of the micro-nano bubble solution 2 in the observation plate (32). S5. Process the video using software to obtain the observation results; S6. The bubble concentration and average diameter of the micro-nano bubble solution were calculated based on the observation results.

8. The observation method according to claim 7, characterized in that, The formula for calculating the total concentration of micro-nano bubbles in the micro-nano bubble solution is as follows: （1）； In equation (1), The total concentration of micro-nano bubbles in the micro-nano bubble solution II, in units of ; The magnification of the optical microscope (21); for The number of all bubbles within the same diameter range as the specified bubble within a given time interval, expressed in units of individual bubbles; The flow rate of the micro / nano bubble solution entering the observation plate (32) is expressed in units of... ; This is the optical detection efficiency factor, with a value range of [value range missing]. ; The actual diameter of the selected bubbles in the micro / nano bubble solution is given in unit . .

9. The observation method according to claim 8, characterized in that, The formula for calculating the concentration of bubbles within the same diameter range as the specified bubbles in the micro / nano bubble solution is as follows: （2）； In equation (2), The concentration of bubbles in the nanobubble solution II that are within the same diameter range as the specified bubbles, in units of... ; The magnification of the optical microscope (21); for The number of all bubbles within the same diameter range as the specified bubble within a given time interval, expressed in units of individual bubbles; The flow rate of the micro / nano bubble solution entering the observation plate (32) is expressed in units of... ; This is the optical detection efficiency factor, with a value range of [value range missing]. .

10. The observation method according to claim 7, characterized in that, In step S3, the collaborative control module (4) regulates the pressure and flow rate of the micro / nano bubble solution, making the pressure as follows: Traffic is Micro-nano bubble solution enters the observation plate (32); In step S4, the duration for which the microscope observation module (2) observes the micro / nano bubble solution II within the observation plate (32) is [duration missing]. , and Within a given time, the rotating structure (31) causes the observation plate (32) to rotate laterally by an angle of _____. The angle of vertical rotation is ; The observation video was used to establish a three-dimensional coordinate system with the selected bubble's position at 0 seconds as the origin. axis, shaft and The axis, analysis yielded the selected bubble in along the time Displacement components in the axial direction Select bubbles along the time Displacement components in the axial direction Select bubbles along the time Displacement components in the axial direction ; The formula for calculating the actual diameter of the selected micro / nano bubble in the micro / nano bubble solution is as follows: （3）； In equation (3), The actual diameter of the selected bubbles in the micro / nano bubble solution is given in unit . ; The Boltzmann constant for the micro / nano bubble solution is given by a value of [value missing]. ; The temperature of the micro / nano bubble solution is given in units of . ; The viscosity of the micro / nano bubble solution is given by a value of [value to be filled in]. ; To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... ; The formula for calculating the diffusion coefficient of selected bubbles in micro / nano bubble solution II is as follows: （4）； In equation (4), To select the diffusion coefficient of bubbles in micro / nano bubble solution II, the unit is... ; For the selected bubbles in The actual displacement within the observation plate (32) relative to the origin at 0 seconds within a second, in units of ; The time taken by the CCD camera (22) in the microscope observation module (2) is in seconds; Selected bubbles in The formula for calculating the actual displacement distance moved on the observation plate (32) within a second is: （5）； In equation (5), For the selected bubbles The actual displacement of the movement on the observation board (32) relative to the origin at 0 seconds within a second, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; Selected bubble Within seconds along The formula for calculating the displacement components in the axial direction is: （6）； In equation (6), a three-dimensional coordinate system is established with the position of the selected bubble at 0 seconds as the origin. axis, shaft and axis; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; For the CCD camera (22) in the microscope observation module (2) on the two-dimensional plane Observations along the axis, in units of ; For the CCD camera (22) in the microscope observation module (2) on the two-dimensional plane Observations along the axis, in units of ; The angle of horizontal rotation of the observation plate (32) is expressed in units of 1. ; Selected bubble Within seconds along The formula for calculating the displacement components in the axial direction is: （7）； In equation (7), the position of the selected bubble at 0 seconds is used as the origin of the coordinate system to establish the x-axis, y-axis and z-axis of the three-dimensional coordinate system; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; The angle of vertical rotation of the observation plate (32) is expressed in units of . ; Selected bubble Within seconds along The formula for calculating the displacement components in the axial direction is: （8）； In equation (8), a three-dimensional coordinate system with the x-axis, y-axis and z-axis is established with the selected position of the bubble at 0 seconds as the origin; To select bubbles in Within seconds along Displacement components in the axial direction, in units of ; Reference grid offset, in units of ; The magnification of the optical microscope (21) in the microscope observation module (2); The angle of vertical rotation of the observation plate (32) is expressed in units of . .

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