A method for promoting the absorption of calcium elements by pineapple fruits

Abstract

The application provides a method for promoting the absorption of calcium elements by pineapple fruits, which comprises the following steps: three months after the pineapple plant is induced to bloom and four months after the pineapple plant is induced to bloom, gauze is soaked in an amino oxyacetic acid aqueous solution or a pyrazine amide aqueous solution, the soaked gauze is applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is secondly soaked in an indole-3-acetic acid aqueous solution, an indole-3-butyric acid aqueous solution, a phenylacetic acid aqueous solution or an alpha-naphthylacetic acid aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is thirdly soaked in a 6-benzylaminopurine aqueous solution, a kinetin aqueous solution, a thidiazuron aqueous solution or a forchlorfenuron aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The application can inhibit the formation of the pineapple fruit stem delimitation, promote the absorption of calcium by the fruits, reduce the incidence of pineapple water core disease from about 18% to about 0.6%, and significantly improve the income of fruit farmers.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, and in particular relates to a method for promoting calcium absorption in pineapple fruits. Background Technology

[0002] Pineapple is the third most widely cultivated tropical fruit in my country, and the pineapple industry plays a vital role in the agricultural economic development of my country's tropical and subtropical regions. Calcium is an essential component of plant cell walls. Calcium deficiency in pineapple fruits easily induces various diseases, such as watercore. Calcium is transported upwards from the soil through the xylem, then in the fruit stalk, from the xylem to the phloem, and finally through the phloem to the fruit. At the junction of the pineapple fruit and stalk, there is a tissue similar to an abscission layer, composed of 3-5 layers of dense cells. In the later stages of fruit development, the cell walls of the abscission layer degrade, and the phloem and xylem break down, making it difficult for calcium ions to be transported from the stalk to the fruit through the abscission layer, resulting in calcium deficiency in the fruit and leading to pineapple diseases. Summary of the Invention

[0003] To address the aforementioned problems, this invention provides a method for promoting calcium absorption in pineapple fruits. This method can significantly reduce the incidence of watercore disease in pineapples and increase farmers' income.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0005] A method for promoting calcium absorption in pineapple fruit involves soaking gauze in an aqueous solution of aminooxyacetic acid or pyrazinamide for the first time three months and four months after flowering of the pineapple plant. The resulting gauze is then applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours to promote calcium absorption in the pineapple fruit.

[0006] Furthermore, the concentration of aminooxyacetic acid in the aqueous solution of aminooxyacetic acid is 0.1~10mM;

[0007] The concentration of pyrazinamide in aqueous solution is 0.2~4mM.

[0008] Furthermore, the method involves soaking gauze in an aqueous solution of aminooxyacetic acid or pyrazinamide for the first time three months and four months after the pineapple plant is induced to flower. The resulting gauze is then applied to the connection between the pineapple fruit and the fruit stalk for 2-5 hours. The gauze is then soaked a second time and applied to the connection between the pineapple fruit and the fruit stalk for another 2-5 hours to promote the absorption of calcium by the pineapple fruit.

[0009] The solution used for the second soaking of the gauze is an aqueous solution of indole-3-acetic acid, an aqueous solution of indole-3-butyric acid, an aqueous solution of phenylacetic acid, or an aqueous solution of α-naphthaleneacetic acid.

[0010] Furthermore, the concentration of indole-3-acetic acid in the aqueous solution of indole-3-acetic acid is 0.1~50 mg / L;

[0011] The concentration of indole-3-butyric acid in aqueous solution is 500~2000 mg / L;

[0012] The concentration of phenylacetic acid in an aqueous solution is 20~50 mg / L;

[0013] The concentration of α-naphthacetic acid in aqueous solution is 10~50 mg / L.

[0014] Furthermore, the method involves soaking gauze in an aqueous solution of aminooxyacetic acid or pyrazinamide for the first time three months and four months after the pineapple plant is induced to flower. The resulting gauze is then applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The gauze is then soaked a second time and applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours again. Finally, the gauze is soaked a third time and applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours to promote the absorption of calcium by the pineapple fruit.

[0015] The solution used for the second soaking of the gauze is an aqueous solution of indole-3-acetic acid, an aqueous solution of indole-3-butyric acid, an aqueous solution of phenylacetic acid, or an aqueous solution of α-naphthaleneacetic acid.

[0016] The solution used for the third soaking of the gauze is an aqueous solution of 6-benzylaminopurine, kinetin, thiamethoxam, or chlorpyrifos.

[0017] Furthermore, the concentration of 6-benzylaminopurine in the aqueous solution is 0.1~5 mg / L;

[0018] The concentration of serokinin in an aqueous solution is 0.5~2 mg / L;

[0019] The concentration of thiamethoxam in aqueous solution is 0.01~0.5 mg / L;

[0020] The concentration of chlorpyrifos in aqueous solution is 5~20 mg / L.

[0021] Furthermore, the method also includes applying calcium magnesium phosphate fertilizer, calcium nitrate, calcium chloride, chelated calcium, or superphosphate to the roots of the pineapple plant.

[0022] The beneficial effects of the method for promoting calcium absorption in pineapple fruit according to the present invention are as follows:

[0023] This invention discloses a method for inhibiting the formation of abscission layer in pineapple fruit stalks and promoting calcium absorption in pineapple fruit, which can reduce the incidence of pineapple watercore disease from about 18% to about 0.6%, significantly increasing fruit farmers' income;

[0024] The difficulty in calcium absorption in pineapple stems from the formation of the abscission layer on the fruit stalk. At this abscission layer, the phloem and xylem break, making it difficult for calcium ions to be transported to the fruit. The formation of the abscission layer is caused by increased ethylene synthesis, which induces increased synthesis and activity of endogenous hydrolytic enzymes in the abscission layer, leading to cell wall degradation and subsequent breakage of the phloem and xylem. This invention uses ethylene inhibitors, aminooxyacetic acid (AOA) and pyrazinamide (PZA), to treat the connection between the pineapple fruit and the fruit stalk. This effectively reduces the ethylene content in the pineapple abscission layer, prevents the breakage of the phloem and xylem, and promotes the transport of calcium ions from the fruit stalk to the fruit.

[0025] Meanwhile, since the abscission layer of pineapple fruit stalks is formed by the differentiation of ordinary parenchyma cells, the use of auxins and cytokinins can effectively inhibit the formation of abscission layer cells. The indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), phenylacetic acid (PAA), and α-naphthaleneacetic acid (NAA) used in this invention, along with 6-benzylaminopurine (6-BA), kinetin (KT), thidiazuron (TDZ), and forchlorfenuron (CPPU), can effectively inhibit the formation of the abscission layer of pineapple fruit stalks and promote the absorption of calcium by pineapple fruit. Attached Figure Description

[0026] Figure 1 This is the pineapple stem abscission layer of the blank control group in Example 1 of the present invention;

[0027] Figure 2 This refers to the pineapple stem abscission layer in the experimental group of Example 1 of the present invention;

[0028] Figure 3 This is a comparison diagram of normal pineapple and water-core pineapple in Embodiment 1 of the present invention, wherein the first four in the first row are water-core pineapples, and the rest are normal pineapples. Detailed Implementation

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Example 1: A method for promoting calcium absorption in pineapple fruit

[0031] This embodiment describes a method for promoting calcium absorption in pineapple fruits, and the specific process includes the following steps:

[0032] From September to October, on well-drained sloping land, deep plow and apply 50 kg of superphosphate per acre, mixed with 500-1000 kg of livestock and poultry manure. Create raised beds and cover with ground cover. Place pineapple seedlings upside down in direct sunlight for a week, then soak the roots and core in a 200-fold dilution of 20% chlorpyrifos and 80% thiamethoxam wettable powder for 5 minutes. After another week of sun exposure, plant on a sunny day. From the time the pineapple plants begin to sprout new leaves until they have 4-5 leaves, apply high-nitrogen compound fertilizer three times, no more than 20-30 kg per acre each time. After the mid-seedling stage, apply fertilizer twice. For the first application, apply 20-30 kg of urea and 10-15 kg of potassium sulfate per acre. For the second application, apply 15-20 kg of urea, 20 kg of potassium sulfate, and 50 kg of superphosphate per acre. When the pineapple plant has 30-40 leaves, with leaves longer than 30 cm, apply a 0.5%-1% solution of calcium carbide to the crown on a clear, rainless night, repeating this twice at two- to three-day intervals. After the flowers turn red, apply a mixture of 20 kg of compound fertilizer and 10 kg of potassium sulfate per acre. After the buds emerge, apply a mixture of 20-30 kg of compound fertilizer and 10 kg of potassium sulfate per acre. At the end of the flowering period, spray with 1% potassium dihydrogen phosphate once. 20-30 days later, spray with 1% potassium chloride once. During the fruit development period, spray with 0.1% potassium nitrate 1-2 times per month and 0.1% calcium magnesium nitrate once per month. Also, apply 30 kg of calcium magnesium phosphate fertilizer per acre to the roots using a fertilizer applicator. It should be noted that the above pineapple planting steps are the standard pineapple planting steps.

[0033] Three months after the pineapple plant was induced to flower, gauze was soaked in a 5mM aminooxyacetic acid (AOA) aqueous solution for the first time. The resulting gauze was then applied to the connection between the pineapple fruit and the stem for 2 hours.

[0034] Then, the gauze was soaked a second time in a 30 mg / L indole-3-acetic acid (IAA) aqueous solution, and the resulting second soaked gauze was applied to the connection between the pineapple fruit and the stem for 2 hours.

[0035] Then, soak the gauze for the third time in a 3 mg / L aqueous solution of 6-benzylaminopurine (6-BA). Apply the resulting soaked gauze to the junction of the pineapple fruit and stem for 2 hours. Apply calcium magnesium phosphate fertilizer to the pineapple roots using a fertilizer applicator at a rate of 30 kg per acre. Alternatively, the same amount of calcium nitrate, calcium chloride, chelated calcium, and superphosphate can be applied instead of calcium magnesium phosphate fertilizer.

[0036] Four months after forcing the flowers to bloom, repeat the above steps once. Then cover the flower with a yellow paper bag.

[0037] The peel changes from green to grass green, and the fruit is harvested when one-quarter of the small fruit turns yellow. The pineapple group treated with the above method is the experimental group.

[0038] The harvested pineapples were longitudinally cut open, and the calcium content of the pulp was measured. The incidence of watercore disease was also statistically analyzed. The experimental group of pineapples had a pulp calcium content of 1.75±0.03 mg / g·DW, a watercore disease incidence rate of 0.8%, and a ethylene content in the pedicel abscission layer of 0.03±0.02 mg / m³. 3 .

[0039] In the specific implementation of the above method, gauze was soaked in clean water for the first to third soakings, and pineapples were treated accordingly. This group served as a blank control group. The calcium content of the pineapple pulp was 1.08±0.04 mg / g·DW, the incidence of watercore was 15%, and the ethylene content of the fruit stalk abscission layer was 0.21±0.02 mg / m³. 3 ;

[0040] Pineapples were treated by soaking gauze in clean water for the first and second times, and then in a 3 mg / L 6-benzylaminopurine aqueous solution for the third time. This group served as the control group. The resulting pineapples had a calcium content of 1.25 ± 0.03 mg / g·DW in the pulp, a watercore incidence rate of 10%, and an ethylene content of 0.16 ± 0.02 mg / m³ in the fruit stalk abscission layer. 3 ;

[0041] Pineapples were treated by first soaking gauze in clean water, then in a 30 mg / L indole-3-acetic acid aqueous solution, and finally in a 3 mg / L 6-benzylaminopurine aqueous solution. This group served as the control group. The resulting pineapples had a calcium content of 1.57 ± 0.03 mg / g·DW in the pulp, a watercore incidence rate of 7%, and an ethylene content of 0.08 ± 0.02 mg / m³ in the fruit stalk abscission layer. 3 .

[0042] Pineapples were treated by first soaking gauze in a 5mM aminooxyacetic acid aqueous solution, and then soaking it in clean water for the second and third times. This group served as the control group. The resulting pineapples had a calcium content of 1.22±0.04 mg / g·DW in the pulp, a watercore incidence rate of 11%, and an ethylene content of 0.16±0.02 mg / m³ in the fruit stalk abscission layer. 3 .

[0043] Pineapples were treated by first soaking gauze in a 5mM aminooxyacetic acid aqueous solution, then in a 30mg / L indole-3-acetic acid aqueous solution, and finally in clean water. This group served as the control group. The resulting pineapples had a calcium content of 1.51±0.03mg / g·DW in the pulp, a watercore incidence rate of 8%, and an ethylene content of 0.09±0.02mg / m³ in the fruit stalk abscission layer. 3 .

[0044] Pineapples were treated by first soaking gauze in a 5mM aminooxyacetic acid aqueous solution, then in clean water, and finally in a 3mg / L 6-benzylaminopurine aqueous solution. This group served as the control group. The resulting pineapples had a calcium content of 1.54±0.03mg / g·DW in the pulp, a watercore incidence rate of 7%, and an ethylene content of 0.08±0.02mg / m³ in the fruit stalk abscission layer. 3 .

[0045] Pineapples were treated by soaking gauze in clean water for the first and third times, and then in a 30 mg / L indole-3-acetic acid aqueous solution for the second time. This group served as the control group. The resulting pineapples had a calcium content of 1.22 ± 0.04 mg / g·DW in the pulp, a watercore incidence rate of 11%, and an ethylene content of 0.16 ± 0.01 mg / m³ in the fruit stalk abscission layer. 3 .

[0046] The comparison between the blank control group and the experimental group showed the following effect: Figure 1 and Figure 2 As shown, where Figure 1 The pineapple pedicel abscission layer, which serves as the blank control group, shows that the xylem and phloem of the pineapple pedicel abscission layer are broken. Figure 2 The image shows the abscission layer of the pineapple stem in the experimental group, indicating that the xylem and phloem of the pineapple stem are intact. This demonstrates that the method of the present invention can effectively reduce the ethylene content in the pineapple stem abscission layer, prevent the breakage of the phloem and xylem in the pineapple stem abscission layer, and promote the transport of calcium ions from the stem to the fruit.

[0047] The comparison chart of normal and watery pineapple fruits between the blank control group and the experimental group is shown below. Figure 3 As shown, the first four fruits in the first row are water-core fruits, and the rest are normal fruits.

[0048] Example 2: A method for promoting calcium absorption in pineapple fruit

[0049] This embodiment describes a method for promoting calcium absorption in pineapple fruits, and the specific process includes the following steps:

[0050] From September to October, on well-drained sloping land, deep plow and apply 50 kg of superphosphate per acre, mixed with 500-1000 kg of livestock and poultry manure. Create raised beds and cover with ground cover. Place pineapple seedlings upside down in direct sunlight for a week, then soak the roots and core in a 200-fold dilution of 20% chlorpyrifos and 80% thiamethoxam wettable powder for 5 minutes. After another week of sun exposure, plant on a sunny day. From the time the pineapple plants begin to sprout new leaves until they have 4-5 leaves, apply high-nitrogen compound fertilizer three times, no more than 20-30 kg per acre each time. After the mid-seedling stage, apply fertilizer twice. For the first application, apply 20-30 kg of urea and 10-15 kg of potassium sulfate per acre. For the second application, apply 15-20 kg of urea, 20 kg of potassium sulfate, and 50 kg of superphosphate per acre. When the pineapple plant has 30-40 leaves, with leaves longer than 30 cm, apply a 0.5%-1% solution of calcium carbide to the crown on a clear, rainless night, repeating this twice at two- to three-day intervals. After the flowers turn red, apply a mixture of 20 kg of compound fertilizer and 10 kg of potassium sulfate per acre. After budding, apply a mixture of 20-30 kg of compound fertilizer and 10 kg of potassium sulfate per acre. At the end of the flowering period, spray with 1% potassium dihydrogen phosphate once. 20-30 days later, spray with 1% potassium chloride once. During fruit development, spray with 0.1% potassium nitrate once or twice a month, and 0.1% calcium magnesium nitrate once a month. Also, apply 30 kg of calcium magnesium phosphate fertilizer per acre to the roots of the pineapple using a fertilizer applicator.

[0051] Three months after the pineapple plant was induced to flower, gauze was soaked in a 3mM pyrazinamide (PZA) aqueous solution for the first time. The resulting gauze was then applied to the connection between the pineapple fruit and the stem for 2 hours.

[0052] Then, the gauze was soaked a second time in a 1000 mg / L indole-3-butyric acid (IBA) aqueous solution, and the resulting second soaked gauze was applied to the connection between the pineapple fruit and the stem for 2 hours.

[0053] Then, soak the gauze for the third time in a 1 mg / L kinetin (KT) aqueous solution. Apply the resulting soaked gauze to the junction of the pineapple fruit and stem for 2 hours. Apply calcium magnesium phosphate fertilizer at a rate of 30 kg per acre to the pineapple roots using a fertilizer applicator.

[0054] Repeat the above procedure once 4 months after the flower-forcing process.

[0055] Then, cover the fruit with a yellow paper bag. The peel changes from green to grass green, and the fruit is harvested when one-quarter of the fruit has turned yellow. The pineapple group treated in the above way serves as the experimental group.

[0056] The harvested pineapples were longitudinally cut open, and the calcium content of the pulp was measured. The incidence of watercore disease was also statistically analyzed. The experimental group of pineapples had a pulp calcium content of 1.77±0.03 mg / g·DW, a watercore disease incidence rate of 0.9%, and a ethylene content in the pedicel abscission layer of 0.02±0.01 mg / m³. 3 .

[0057] In the specific implementation of the above method, gauze was soaked in clean water for the first to third soakings, and pineapples were treated accordingly. This group served as a blank control group. The calcium content of the pineapple pulp was 1.03±0.04 mg / g·DW, the incidence of watercore was 16%, and the ethylene content in the abscission layer of the fruit stalk was 0.21±0.01 mg / m³. 3 ;

[0058] Pineapples were treated by soaking gauze in clean water for the first and second times, and then in a 1 mg / L kinetin aqueous solution for the third time. This group served as the control group. The resulting pineapples had a calcium content of 1.23 ± 0.04 mg / g·DW in the pulp, a watercore incidence rate of 11%, and an ethylene content of 0.16 ± 0.01 mg / m³ in the fruit stalk abscission layer. 3 ;

[0059] Pineapples were treated by first soaking gauze in clean water, then in a 1000 mg / L indole-3-butyric acid aqueous solution, and finally in a 1 mg / L kinetin aqueous solution. This group served as the control group. The resulting pineapples had a calcium content of 1.55 ± 0.05 mg / g·DW in the pulp, a watercore incidence rate of 8%, and an ethylene content of 0.10 ± 0.02 mg / m³ in the fruit stalk abscission layer. 3 ;

[0060] Pineapples were treated by first soaking gauze in a 3mM pyrazinamide aqueous solution, and then soaking it in clean water for the second and third times. This group served as the control group. The resulting pineapples had a calcium content of 1.22±0.03 mg / g·DW in the pulp, a watercore incidence rate of 10%, and an ethylene content of 0.16±0.02 mg / m³ in the fruit stalk abscission layer. 3 .

[0061] Pineapples were treated by first soaking gauze in a 3mM pyrazinamide aqueous solution, then in a 1000mg / L indole-3-butyric acid aqueous solution, and finally in clean water. This group served as the control group. The resulting pineapples had a calcium content of 1.51±0.05mg / g·DW in the pulp, a watercore incidence rate of 7%, and an ethylene content of 0.09±0.02mg / m³ in the fruit stalk abscission layer. 3 .

[0062] Pineapples were treated by first soaking gauze in a 3mM pyrazinamide aqueous solution, then in clean water, and finally in a 1mg / L kinetin aqueous solution. This group served as a control group. The resulting pineapples had a calcium content of 1.48±0.03mg / g·DW in the pulp, a watercore incidence rate of 6%, and an ethylene content of 0.08±0.01mg / m³ in the fruit stalk abscission layer. 3 .

[0063] Pineapples were treated by soaking gauze in clean water for the first and third times, and then in a 1000 mg / L indole-3-butyric acid aqueous solution for the second time. This group served as the control group. The resulting pineapples had a calcium content of 1.19 ± 0.03 mg / g·DW in the pulp, a watercore incidence rate of 10%, and an ethylene content of 0.16 ± 0.01 mg / m³ in the fruit stalk abscission layer. 3 .

[0064] Example 3: A method for promoting calcium absorption in pineapple fruit

[0065] This embodiment describes a method for promoting calcium absorption in pineapple fruits, and the specific process includes the following steps:

[0066] From September to October, on well-drained sloping land, deep plow and apply 50 kg of superphosphate per acre, mixed with 500-1000 kg of livestock and poultry manure. Create raised beds and cover with ground cover. Place pineapple seedlings upside down in direct sunlight for a week, then soak the roots and core in a 200-fold dilution of 20% chlorpyrifos and 80% thiamethoxam wettable powder for 5 minutes. After another week of sun exposure, plant on a sunny day. From the time the pineapple plants begin to sprout new leaves until they have 4-5 leaves, apply high-nitrogen compound fertilizer three times, no more than 20-30 kg per acre each time. After the mid-seedling stage, apply fertilizer twice. For the first application, apply 20-30 kg of urea and 10-15 kg of potassium sulfate per acre. For the second application, apply 15-20 kg of urea, 20 kg of potassium sulfate, and 50 kg of superphosphate per acre. When the pineapple plant has 30-40 leaves, with leaves longer than 30 cm, apply a 0.5%-1% solution of calcium carbide to the crown on a clear, rainless night, repeating this twice at two- to three-day intervals. After the flowers turn red, apply a mixture of 20 kg of compound fertilizer and 10 kg of potassium sulfate per acre. After budding, apply a mixture of 20-30 kg of compound fertilizer and 10 kg of potassium sulfate per acre. At the end of the flowering period, spray with 1% potassium dihydrogen phosphate once. 20-30 days later, spray with 1% potassium chloride once. During fruit development, spray with 0.1% potassium nitrate once or twice a month, and 0.1% calcium magnesium nitrate once a month. Also, apply 30 kg of calcium magnesium phosphate fertilizer per acre to the roots of the pineapple using a fertilizer applicator.

[0067] Three months after the pineapple plant was induced to flower, gauze was soaked in a 5mM aminooxyacetic acid (AOA) aqueous solution for the first time. The resulting gauze was then applied to the connection between the pineapple fruit and the stem for 2 hours.

[0068] Then, the gauze was soaked a second time in a 30 mg / L phenylacetic acid (PAA) aqueous solution, and the resulting second soaked gauze was applied to the connection between the pineapple fruit and the stem for 2 hours.

[0069] Then, soak the gauze for the third time in a 0.3 mg / L thiadiazuron (TDZ) aqueous solution. Apply the resulting gauze, after the third soaking, to the junction of the pineapple fruit and the stem for 2 hours. Apply calcium magnesium phosphate fertilizer at a rate of 30 kg per acre to the pineapple roots using a fertilizer applicator.

[0070] Repeat the above procedure once 4 months after the flower-forcing process.

[0071] Then, cover the fruit with a yellow paper bag. The peel changes from green to grass green, and the fruit is harvested when one-quarter of the fruit has turned yellow. The pineapple group treated in the above way serves as the experimental group.

[0072] The harvested pineapples were longitudinally cut open, and the calcium content of the pulp was measured. The incidence of watercore disease was also statistically analyzed. The experimental group of pineapples had a pulp calcium content of 1.81±0.04 mg / g·DW, a watercore disease incidence of 0.7%, and an ethylene content of 0.04±0.01 mg / m³ in the pedicel abscission layer. 3 .

[0073] In the specific implementation of the above method, gauze was soaked in clean water for the first to third soakings, and pineapples were treated accordingly. This group served as a blank control group. The calcium content of the pineapple pulp was 1.01±0.03 mg / g·DW, the incidence of watercore was 18%, and the ethylene content in the abscission layer of the fruit stalk was 0.21±0.02 mg / m³. 3 ;

[0074] Pineapples were treated by soaking gauze in clean water for the first and second times, and then in a 0.3 mg / L thiamethoxam aqueous solution for the third time. This group served as a control group. The resulting pineapples had a calcium content of 1.21 ± 0.04 mg / g·DW in the pulp, a watercore incidence rate of 12%, and an ethylene content of 0.16 ± 0.02 mg / m³ in the abscission layer of the fruit stalk. 3 ;

[0075] Pineapples were treated by soaking gauze in water, then in a 30 mg / L phenylacetic acid solution, and finally in a 0.3 mg / L thidiazuron solution. This group served as the control group. The resulting pineapples had a calcium content of 1.51 ± 0.03 mg / g·DW in the pulp, a watercore incidence rate of 6%, and an ethylene content of 0.09 ± 0.01 mg / m³ in the fruit stalk abscission layer. 3 ;

[0076] Pineapples were treated by first soaking gauze in a 5mM aminooxyacetic acid aqueous solution, and then soaking it in clean water for the second and third times. This group served as the control group. The resulting pineapples had a calcium content of 1.19±0.03 mg / g·DW in the pulp, a watercore incidence rate of 11%, and an ethylene content of 0.16±0.02 mg / m³ in the fruit stalk abscission layer. 3 .

[0077] Pineapples were treated by first soaking gauze in a 5mM aminooxyacetic acid aqueous solution, then in a 30mg / L phenylacetic acid aqueous solution, and finally in clean water. This group served as the control group. The resulting pineapples had a calcium content of 1.56±0.03mg / g·DW in the pulp, a watercore incidence rate of 5%, and an ethylene content of 0.09±0.02mg / m³ in the fruit stalk abscission layer. 3 .

[0078] Pineapples were treated by first soaking gauze in a 5mM aminooxyacetic acid aqueous solution, then in clean water, and finally in a 0.3mg / L thidiazuron aqueous solution. This group served as the control group. The resulting pineapples had a calcium content of 1.54±0.04mg / g·DW in the pulp, a watercore incidence rate of 7%, and an ethylene content of 0.10±0.02mg / m³ in the fruit stalk abscission layer. 3 .

[0079] Pineapples were treated by soaking gauze in clean water for the first and third times, and then in a 30 mg / L phenylacetic acid aqueous solution for the second time. This group served as the control group. The resulting pineapples had a calcium content of 1.19 ± 0.03 mg / g·DW in the pulp, a watercore incidence rate of 13%, and an ethylene content of 0.15 ± 0.02 mg / m³ in the fruit stalk abscission layer. 3 .

[0080] Example 4: A method for promoting calcium absorption in pineapple fruit

[0081] This embodiment describes a method for promoting calcium absorption in pineapple fruits, and the specific process includes the following steps:

[0082] From September to October, on well-drained sloping land, deep plow and apply 50 kg of superphosphate per acre, mixed with 500-1000 kg of livestock and poultry manure. Create raised beds and cover with ground cover. Place pineapple seedlings upside down in direct sunlight for a week, then soak the roots and core in a 200-fold dilution of 20% chlorpyrifos and 80% thiamethoxam wettable powder for 5 minutes. After another week of sun exposure, plant on a sunny day. From the time the pineapple plants begin to sprout new leaves until they have 4-5 leaves, apply high-nitrogen compound fertilizer three times, no more than 20-30 kg per acre each time. After the mid-seedling stage, apply fertilizer twice. For the first application, apply 20-30 kg of urea and 10-15 kg of potassium sulfate per acre. For the second application, apply 15-20 kg of urea, 20 kg of potassium sulfate, and 50 kg of superphosphate per acre. When the pineapple plant has 30-40 leaves, with leaves longer than 30 cm, apply a 0.5%-1% solution of calcium carbide to the crown on a clear, rainless night, repeating this twice at two- to three-day intervals. After the flowers turn red, apply a mixture of 20 kg of compound fertilizer and 10 kg of potassium sulfate per acre. After budding, apply a mixture of 20-30 kg of compound fertilizer and 10 kg of potassium sulfate per acre. At the end of the flowering period, spray with 1% potassium dihydrogen phosphate once. 20-30 days later, spray with 1% potassium chloride once. During fruit development, spray with 0.1% potassium nitrate once or twice a month, and 0.1% calcium magnesium nitrate once a month. Also, apply 30 kg of calcium magnesium phosphate fertilizer per acre to the roots of the pineapple using a fertilizer applicator.

[0083] Three months after the pineapple plant was induced to flower, gauze was soaked in a 3mM pyrazinamide (PZA) aqueous solution for the first time. The resulting gauze was then applied to the connection between the pineapple fruit and the stem for 2 hours.

[0084] Then, the gauze was soaked a second time in a 30 mg / L aqueous solution of α-naphthaleneacetic acid (NAA). The resulting gauze, after the second soaking, was then applied to the connection between the pineapple fruit and the stem for 2 hours.

[0085] Then, soak the gauze for the third time in a 10 mg / L forchlorfenuron (CPPU) aqueous solution. Apply the resulting soaked gauze to the junction of the pineapple fruit and stem for 2 hours. Apply calcium magnesium phosphate fertilizer at a rate of 30 kg per acre to the pineapple roots using a fertilizer applicator.

[0086] Repeat the above procedure once 4 months after the flower-forcing process.

[0087] Then, cover the fruit with a yellow paper bag. The peel changes from green to grass green, and the fruit is harvested when one-quarter of the fruit has turned yellow. The pineapple group treated in the above way serves as the experimental group.

[0088] The harvested pineapples were longitudinally cut open, and the calcium content of the pulp was measured. The incidence of watercore disease was also statistically analyzed. The experimental group's pineapples had a pulp calcium content of 1.85±0.04 mg / g·DW, a watercore disease incidence of 0.6%, and an ethylene content of 0.02±0.01 mg / m³ in the pedicel abscission layer. 3 .

[0089] In the specific implementation of the above method, gauze was soaked in clean water for the first to third soakings, and pineapples were treated accordingly. This group served as a blank control group. The calcium content of the pineapple pulp was 1.01±0.04 mg / g·DW, the incidence of watercore was 17%, and the ethylene content of the fruit stalk abscission layer was 0.20±0.01 mg / m³. 3 ;

[0090] Pineapples were treated by soaking gauze in clean water for the first and second times, and then in a 10 mg / L chlorpyrifos aqueous solution for the third time. This group served as the control group. The resulting pineapples had a calcium content of 1.18 ± 0.03 mg / g·DW in the pulp, a watercore incidence rate of 11%, and an ethylene content of 0.16 ± 0.01 mg / m³ in the fruit stalk abscission layer. 3 ;

[0091] Pineapples were treated by soaking gauze in clean water for the first time, then in a 30 mg / L α-naphthaleneacetic acid aqueous solution for the second time, and finally in a 10 mg / L chlorpyrifos aqueous solution. This group served as the control group. The resulting pineapples had a calcium content of 1.59 ± 0.04 mg / g·DW in the pulp, a watercore incidence rate of 5%, and an ethylene content of 0.09 ± 0.02 mg / m³ in the fruit stalk abscission layer. 3 ;

[0092] Pineapples were treated by first soaking gauze in a 3mM pyrazinamide aqueous solution, and then soaking it in clean water for the second and third times. This group served as the control group. The resulting pineapples had a calcium content of 1.20±0.03 mg / g·DW in the pulp, a watercore incidence rate of 12%, and an ethylene content of 0.15±0.02 mg / m³ in the fruit stalk abscission layer. 3 .

[0093] Pineapples were treated by first soaking gauze in a 3mM pyrazinamide aqueous solution, then in a 30mg / L α-naphthaleneacetic acid aqueous solution, and finally in clean water. This group served as the control group. The resulting pineapples had a calcium content of 1.62±0.04mg / g·DW in the pulp, a watercore incidence rate of 5%, and an ethylene content of 0.10±0.02mg / m³ in the fruit stalk abscission layer. 3 .

[0094] Pineapples were treated by first soaking gauze in a 3mM pyrazinamide aqueous solution, then in clean water, and finally in a 10mg / L chlorpyrifos aqueous solution. This group served as the control group. The resulting pineapples had a calcium content of 1.63±0.03mg / g·DW in the pulp, a watercore incidence rate of 4%, and an ethylene content of 0.08±0.01mg / m³ in the fruit stalk abscission layer. 3 .

[0095] Pineapples were treated by soaking gauze in clean water for the first and third times, and then in a 30 mg / L α-naphthaleneacetic acid aqueous solution for the second time. This group served as the control group. The resulting pineapples had a calcium content of 1.16 ± 0.05 mg / g·DW in the pulp, a watercore incidence rate of 12%, and an ethylene content of 0.15 ± 0.01 mg / m³ in the fruit stalk abscission layer. 3 .

[0096] Examples 5-6: Methods to promote calcium absorption in pineapple fruit

[0097] Examples 5 and 6 are methods for promoting calcium absorption in pineapple fruits. Their preparation steps are basically the same as in Example 1, differing only in the proportions of the raw materials. See Table 1 for details.

[0098] Table 1. Summary of process parameters in Examples 5 and 6

[0099]

[0100] The contents of Examples 5 and 6 are the same as those of the experimental group in Example 1.

[0101] Examples 7-8: Methods to promote calcium absorption in pineapple fruit

[0102] Examples 7 and 8 are methods for promoting calcium absorption in pineapple fruits. Their preparation steps are basically the same as in Example 2, differing only in the proportions of the raw materials. See Table 2 for details.

[0103] Table 2. Summary of process parameters in Examples 7 and 8

[0104]

[0105] The contents of Examples 7 and 8 are the same as those of the experimental group in Example 2.

[0106] Examples 9-10: Methods to promote calcium absorption in pineapple fruit

[0107] Examples 9 and 10 are methods for promoting calcium absorption in pineapple fruits. Their preparation steps are basically the same as in Example 3, differing only in the proportions of the raw materials. See Table 3 for details.

[0108] Table 3. Summary of process parameters in Examples 9 and 10

[0109]

[0110] The contents of Examples 9 and 10 are the same as those of the experimental group in Example 3.

[0111] Examples 11-12: Methods to promote calcium absorption in pineapple fruit

[0112] Examples 11 and 12 are methods for promoting calcium absorption in pineapple fruits. Their preparation steps are basically the same as in Example 4, differing only in the proportions of the raw materials. See Table 4 for details.

[0113] Table 4. Summary of process parameters in Examples 11-12

[0114]

[0115] The contents of Examples 11 and 12 are the same as those of the experimental group in Example 4.

[0116] Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A method for promoting calcium absorption in pineapple fruit, characterized in that, The method involves soaking gauze in a 0.1-10 mM aminooxyacetic acid aqueous solution or a 0.2-4 mM pyrazinamide aqueous solution three months after the pineapple plant is induced to flower. The resulting gauze is then applied to the connection between the pineapple fruit and the fruit stalk for 2-5 hours to promote the absorption of calcium by the pineapple fruit. Four months after the flowering is induced, the gauze is soaked for the first time in an aqueous solution of aminooxyacetic acid with a concentration of 0.1~10mM or an aqueous solution of pyrazinamide with a concentration of 0.2~4mM. The resulting gauze is then applied to the connection between the pineapple fruit and the fruit stem for 2~5 hours to promote the absorption of calcium by the pineapple fruit.

2. The method for promoting calcium absorption in pineapple fruit according to claim 1, characterized in that, The method involves soaking gauze in an aqueous solution of aminooxyacetic acid or pyrazinamide three months after the pineapple plant has been induced to flower. The resulting gauze is then applied to the connection between the pineapple fruit and the stem for 2-5 hours. The gauze is then soaked a second time and applied to the connection between the pineapple fruit and the stem for another 2-5 hours to promote calcium absorption by the pineapple fruit. Four months after the flowering is induced, the gauze is soaked in an aqueous solution of aminooxyacetic acid or pyrazinamide for the first time. The resulting gauze is then applied to the connection between the pineapple fruit and the stem for 2-5 hours. The gauze is then soaked a second time and applied to the connection between the pineapple fruit and the stem for another 2-5 hours to promote the absorption of calcium by the pineapple fruit. The solution used for the second soaking of the gauze is an aqueous solution of indole-3-acetic acid, an aqueous solution of indole-3-butyric acid, an aqueous solution of phenylacetic acid, or an aqueous solution of α-naphthaleneacetic acid.

3. The method for promoting calcium absorption in pineapple fruit according to claim 2, characterized in that, The concentration of indole-3-acetic acid in aqueous solution is 0.1~50 mg / L; The concentration of indole-3-butyric acid in aqueous solution is 500~2000 mg / L; The concentration of phenylacetic acid in an aqueous solution is 20~50 mg / L; The concentration of α-naphthacetic acid in aqueous solution is 10~50 mg / L.

4. The method for promoting calcium absorption in pineapple fruit according to claim 1, characterized in that, The method involves soaking gauze in an aqueous solution of aminooxyacetic acid or pyrazinamide three months after the pineapple plant has been induced to flower. The resulting gauze is then applied to the connection between the pineapple fruit and the stem for 2-5 hours. The gauze is then soaked a second time and applied to the connection between the pineapple fruit and the stem for another 2-5 hours. Finally, the gauze is soaked a third time and applied to the connection between the pineapple fruit and the stem for another 2-5 hours to promote calcium absorption by the pineapple fruit. Four months after the flowering is induced, the gauze is soaked in an aqueous solution of aminooxyacetic acid or pyrazinamide for the first time. The resulting gauze is then applied to the connection between the pineapple fruit and the stem for 2-5 hours. The gauze is then soaked a second time and applied to the connection between the pineapple fruit and the stem for 2-5 hours. The gauze is then soaked a third time and applied to the connection between the pineapple fruit and the stem for 2-5 hours to promote the absorption of calcium by the pineapple fruit. The solution used for the second soaking of the gauze is an aqueous solution of indole-3-acetic acid, an aqueous solution of indole-3-butyric acid, an aqueous solution of phenylacetic acid, or an aqueous solution of α-naphthaleneacetic acid. The solution used for the third soaking of the gauze is an aqueous solution of 6-benzylaminopurine, kinetin, thiamethoxam, or chlorpyrifos.

5. The method for promoting calcium absorption in pineapple fruit according to claim 4, characterized in that, The concentration of 6-benzylaminopurine in aqueous solution is 0.1~5 mg / L; The concentration of serokinin in an aqueous solution is 0.5~2 mg / L; The concentration of thiamethoxam in aqueous solution is 0.01~0.5 mg / L; The concentration of chlorpyrifos in aqueous solution is 5~20 mg / L.

6. The method for promoting calcium absorption in pineapple fruit according to any one of claims 1-5, characterized in that, The method also includes applying calcium magnesium phosphate fertilizer, calcium nitrate, calcium chloride, chelated calcium, or superphosphate to the roots of pineapple plants.

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