Natural plant extract antibacterial mouthwash and preparation method thereof

Abstract

The invention relates to natural plant extract antibacterial mouthwash and a preparation method thereof, and belongs to the technical field of mouthwash. The mouthwash takes water as a solvent and is composed of natural plant extracts and auxiliary functional auxiliaries, the natural plant extracts comprise honeysuckle flower, liquorice and tea leaf extracts, and the mouthwash is natural and safe, can effectively inhibit bacteria and overcomes the defects of traditional chemical mouthwash. Meanwhile, the natural plant extract is modified by the casein phosphopeptide and the tannic acid, the tannic acid is cross-linked with the casein phosphopeptide and the natural plant extract through phenolic hydroxyl groups to form a stable compound, and the casein phosphopeptide is combined with calcium ions in oral mucosa or enamel, so that the residence time of the natural plant extract in the oral cavity is prolonged, and the oral cavity health care effect is improved. Therefore, the antibacterial effect of the mouthwash is improved.

Description

Technical Field

[0001] This invention belongs to the field of mouthwash technology, and relates to a natural plant extract antibacterial mouthwash and its preparation method. Background Technology

[0002] In today's society, people are paying increasing attention to oral hygiene and health. Mouthwash, as an important product for daily oral cleaning and care, is experiencing continuous market demand growth. Traditional mouthwashes typically contain chemical antibacterial agents and organic solvents such as chlorhexidine and alcohol. While these chemicals can effectively inhibit the growth of bacteria in the mouth and achieve good antibacterial and cleaning effects in the short term, long-term use can lead to many problems. For example, chlorhexidine can cause staining of teeth and mucous membranes, disrupt the ecological balance of normal oral flora, and increase the risk of oral fungal infections; alcohol can easily irritate the oral mucosa, causing discomfort such as dry mouth and burning sensations, especially for people with sensitive mouths, resulting in a poor user experience. Summary of the Invention

[0003] The purpose of this invention is to provide a natural plant extract antibacterial mouthwash and its preparation method. The mouthwash of this invention uses natural plant extracts as antibacterial ingredients and has a good antibacterial effect.

[0004] The objective of this invention can be achieved through the following technical solutions: In a first aspect, the present invention provides a natural plant extract antibacterial mouthwash, comprising the following ingredients: natural plant extract, auxiliary functional agent and water; The natural plant extracts include honeysuckle flower extract, licorice extract, and tea extract.

[0005] Preferably, the natural plant extract is modified with casein phosphopeptide and tannic acid.

[0006] Preferably, the modification steps of the natural plant extract are as follows: Honeysuckle flower extract, licorice extract, tea extract and water were mixed, casein phosphopeptide was added, and the mixture was stirred evenly. Then tannic acid was added to form a complex.

[0007] Preferably, the mass ratio of casein phosphopeptide, tannic acid and natural plant extract is 1-5:0.5-2.0:100.

[0008] Preferably, in the modification step of the natural plant extract, after adding tannic acid solution, the pH is controlled to be 4.5-6.0.

[0009] Preferably, in the modification process of the natural plant extract, after adding the tannic acid solution, the temperature is controlled at 40-60℃.

[0010] Preferably, the modified natural plant extract accounts for 0.03-0.3% of the mass percentage of the mouthwash raw material.

[0011] Preferably, the auxiliary functional agent includes one or more of propylene glycol, malic acid, xylitol, menthol, and benzoic acid.

[0012] Preferably, the propylene glycol accounts for 0.01-15% of the mouthwash ingredients by mass, the malic acid accounts for 0.001-0.1% of the mouthwash ingredients by mass, the xylitol accounts for 0.01-3% of the mouthwash ingredients by mass, the menthol accounts for 0.01-0.5% of the mouthwash ingredients by mass, and the benzoic acid accounts for 0.01-0.5% of the mouthwash ingredients by mass.

[0013] Secondly, the present invention provides a method for preparing a natural plant extract antibacterial mouthwash as described above, comprising the following steps: Add auxiliary functional agents and modified natural plant extracts to water step by step, stir and mix to obtain the finished mouthwash.

[0014] The beneficial effects of this invention are: 1. This invention's mouthwash uses natural plant extracts such as honeysuckle, licorice, and tea as its core antibacterial ingredients. It abandons the chemical antibacterial agents and organic solvents commonly used in traditional chemical mouthwashes, such as chlorhexidine and alcohol. This avoids the potential side effects of chemical substances, such as tooth and mucosal staining, imbalance of normal oral flora, and mucosal irritation. It is gentle and non-irritating to the oral cavity, and is especially suitable for people with sensitive mouths. The safety and comfort of use are significantly improved.

[0015] 2. Natural plant extracts are modified with casein phosphopeptides and tannic acid. Tannic acid cross-links with casein phosphopeptides and natural plant extracts through phenolic hydroxyl groups to form a stable complex, thus achieving a composite of casein phosphopeptides and natural plant extracts. Casein phosphopeptides can bind to calcium ions in the oral mucosa or tooth enamel, thereby prolonging the retention time of natural plant extracts in the oral cavity. This allows the antibacterial components to exert their effects more persistently and fully, significantly improving the antibacterial effect of the mouthwash, effectively inhibiting bacterial growth in the oral cavity, and maintaining a healthy oral environment. Detailed Implementation

[0016] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0017] The honeysuckle flower extracts used in the embodiments and comparative examples of this invention were purchased from Sanyang Lianfeng Biotechnology Co., Ltd.

[0018] The licorice extracts used in the embodiments and comparative examples of this invention were purchased from Xi'an An'ao Biotechnology Co., Ltd.

[0019] The tea extracts used in the embodiments and comparative examples of this invention are selected from green tea extracts and purchased from Nanjing Tongying Biotechnology Co., Ltd.

[0020] Example 1 A natural plant extract antibacterial mouthwash comprises the following ingredients by weight percentage, as shown in Table 1.

[0021] Table 1 The natural plant extracts include honeysuckle flower extract, licorice extract, and tea extract, with a mass ratio of 1:1:1.

[0022] Natural plant extracts are modified with casein phosphopeptides and tannic acid.

[0023] The specific steps for modifying natural plant extracts are as follows: Weigh out the following ingredients in the specified proportions: honeysuckle flower extract, licorice extract, tea extract, casein phosphopeptide, and tannic acid. Add a portion of the water from the antibacterial mouthwash ingredients. The mass ratio of casein phosphopeptide, tannic acid, and natural plant extracts is 1:0.5:100, and the amount of water added is 90% of the total mass of the modified natural plant extract reaction system. Place the honeysuckle flower extract, licorice extract, tea extract, and water in a clean container and mix thoroughly. Add the casein phosphopeptide and stir with a magnetic stirrer at 200 rpm for 10 minutes to ensure thorough mixing with the natural plant extracts. Next, add tannic acid and adjust the pH of the system to 4.5 (using a 0.1 mol / L hydrochloric acid solution). Finally, place the reaction vessel in a 40°C constant temperature water bath and react at 150 rpm for 2 hours to form a complex. Cool to room temperature for later use.

[0024] The specific preparation method of a natural plant extract antibacterial mouthwash is as follows: Weigh the raw materials according to the proportions, and reserve some water as needed for the modification of natural plant extracts. Then, add water, xylitol, and propylene glycol to the emulsifier / mixing tank in the correct proportions. Turn on the emulsifier / mixing tank, stir at 25 r / min, heat to 85℃±5℃, keep warm for 20 minutes, and then stir to cool down to 40±5℃. Next, after modifying the natural plant extracts, add the modified natural plant extracts to the emulsifier / mixing tank and stir at 25 r / min for 10 minutes. Then add propylene glycol, menthol, and benzoic acid and stir at 25 r / min for 10 minutes to obtain the antibacterial mouthwash made from natural plant extracts.

[0025] Example 2 A natural plant extract antibacterial mouthwash comprises the following ingredients by weight percentage, as shown in Table 2.

[0026] Table 2 The natural plant extracts include honeysuckle flower extract, licorice extract, and tea extract, with a mass ratio of 1:1:1.

[0027] Natural plant extracts are modified with casein phosphopeptides and tannic acid.

[0028] The specific steps for modifying natural plant extracts are as follows: Weigh out the following ingredients in the specified proportions: honeysuckle flower extract, licorice extract, tea extract, casein phosphopeptide, and tannic acid. Add a portion of the water from the antibacterial mouthwash raw material. The mass ratio of casein phosphopeptide, tannic acid, and natural plant extracts is 4:1:100, and the amount of water added is 90% of the total mass of the modified natural plant extract reaction system. Place the honeysuckle flower extract, licorice extract, tea extract, and water in a clean container and mix thoroughly. Add casein phosphopeptide and stir with a magnetic stirrer at 200 rpm for 10 minutes to ensure thorough mixing with the natural plant extracts. Next, add tannic acid and adjust the pH of the system to 5 (using a 0.1 mol / L citric acid solution). Finally, place the reaction vessel in a 50°C constant temperature water bath and react at 150 rpm for 1.8 hours to form a complex. Cool to room temperature for later use.

[0029] The specific preparation method of a natural plant extract antibacterial mouthwash is as follows: Weigh the raw materials according to the proportions, and reserve some water as needed for the modification of natural plant extracts. Then, add water, xylitol, and propylene glycol to the emulsifier / mixing tank in the correct proportions. Turn on the emulsifier / mixing tank, stir at 25 r / min, heat to 85℃±5℃, keep warm for 20 minutes, and then stir to cool down to 40±5℃. Next, after modifying the natural plant extracts, add the modified natural plant extracts to the emulsifier / mixing tank and stir at 25 r / min for 10 minutes. Then add propylene glycol, menthol, and benzoic acid and stir at 25 r / min for 10 minutes to obtain the antibacterial mouthwash made from natural plant extracts.

[0030] Example 3 A natural plant extract antibacterial mouthwash comprises the following ingredients by weight percentage, as shown in Table 3.

[0031] Table 3 The natural plant extracts include honeysuckle flower extract, licorice extract, and tea extract, with a mass ratio of 1:1:1.

[0032] Natural plant extracts are modified with casein phosphopeptides and tannic acid.

[0033] The specific steps for modifying natural plant extracts are as follows: Weigh out the following ingredients in the specified proportions: honeysuckle flower extract, licorice extract, tea extract, casein phosphopeptide, and tannic acid. Add a portion of the water from the antibacterial mouthwash ingredients. The mass ratio of casein phosphopeptide, tannic acid, and natural plant extracts is 5:2:100, and the amount of water added is 90% of the total mass of the modified natural plant extract reaction system. Place the honeysuckle flower extract, licorice extract, tea extract, and water in a clean container and mix thoroughly. Add the casein phosphopeptide and stir with a magnetic stirrer at 200 rpm for 10 minutes to ensure thorough mixing with the natural plant extracts. Next, add tannic acid and adjust the pH of the system to 6 (using a 0.1 mol / L sodium hydroxide solution). Finally, place the reaction vessel in a 60°C constant temperature water bath and react at 150 rpm for 1.5 hours to form a complex. Cool to room temperature for later use.

[0034] The specific preparation method of a natural plant extract antibacterial mouthwash is as follows: Weigh the raw materials according to the proportions, and reserve some water as needed for the modification of natural plant extracts. Then, add water, xylitol, and propylene glycol to the emulsifier / mixing tank in the correct proportions. Turn on the emulsifier / mixing tank, stir at 25 r / min, heat to 85℃±5℃, keep warm for 20 minutes, and then stir to cool down to 40±5℃. Next, after modifying the natural plant extracts, add the modified natural plant extracts to the emulsifier / mixing tank and stir at 25 r / min for 10 minutes. Then add propylene glycol, menthol, and benzoic acid and stir at 25 r / min for 10 minutes to obtain the antibacterial mouthwash made from natural plant extracts.

[0035] Example 4 A natural plant extract antibacterial mouthwash comprises the following ingredients by weight percentage, as shown in Table 4.

[0036] Table 4 The natural plant extracts include honeysuckle flower extract, licorice extract, and tea extract. The mass ratio of honeysuckle flower extract, licorice extract, and tea extract is 1:1:1.

[0037] Natural plant extracts are modified with casein phosphopeptides and tannic acid.

[0038] The specific steps for modifying natural plant extracts are as follows: Weigh out the following ingredients in the specified proportions: honeysuckle flower extract, licorice extract, tea extract, casein phosphopeptide, and tannic acid. Add a portion of the water from the antibacterial mouthwash ingredients. The mass ratio of casein phosphopeptide, tannic acid, and natural plant extracts is 1:1:100, and the amount of water added is 90% of the total mass of the modified natural plant extract reaction system. Place the honeysuckle flower extract, licorice extract, tea extract, and water in a clean container and mix thoroughly. Add casein phosphopeptide and stir with a magnetic stirrer at 200 rpm for 10 minutes to ensure thorough mixing with the natural plant extracts. Next, add tannic acid and adjust the pH of the system to 4.8 (using a 0.1 mol / L citric acid solution). Finally, place the reaction vessel in a 40°C constant temperature water bath and react at 150 rpm for 2 hours to form a complex. Cool to room temperature for later use.

[0039] The specific preparation method of a natural plant extract antibacterial mouthwash is as follows: Weigh the raw materials according to the proportions, and reserve some water as needed for the modification of natural plant extracts. Then, add water, xylitol, and propylene glycol to the emulsifier / mixing tank in the correct proportions. Turn on the emulsifier / mixing tank, stir at 25 r / min, heat to 85℃±5℃, keep warm for 20 minutes, and then stir to cool down to 40±5℃. Next, after modifying the natural plant extracts, add the modified natural plant extracts to the emulsifier / mixing tank and stir at 25 r / min for 10 minutes. Then add propylene glycol, menthol, and benzoic acid and stir at 25 r / min for 10 minutes to obtain the antibacterial mouthwash made from natural plant extracts.

[0040] Comparative Example 1 The difference from Example 3 is that the natural plant extract was not modified.

[0041] Comparative Example 2 The difference from Example 3 is that in the specific steps of the modification treatment of natural plant extracts, the prepared tannic acid aqueous solution is slowly added to mixture c, and the pH of the system is adjusted to 7.0.

[0042] test Subjects: Sixty volunteers were selected. Volunteers were required to meet the following criteria: healthy adults aged 20-25 years, with no oral diseases, no history of drug allergies, and who fasted, abstained from water, smoking, alcohol, and fluoride toothpaste for one hour prior to the test. After screening, the volunteers were randomly divided into six groups: A, B, C, D, E, and F, with 10 volunteers in each group.

[0043] Test steps: 1. Each volunteer in groups A, B, C, D, E, and F used the mouthwash prepared in Examples 1-4, Comparative Examples 1 and 2, with a specific dosage of 30 ml. Participants were required to strictly follow the group assignment instructions, rinsing their mouths for 2 minutes with the designated mouthwash and spitting it out immediately after rinsing. 2. Sample collection should begin immediately after rinsing. For saliva collection, subjects should spit naturally secreted saliva into a sterile container, with the sampling volume strictly controlled to 3 mL. For tooth / mucosal sampling, sterile cotton swabs should be used to gently scrape away residue from the tooth surface (buccal and lingual sides) and mucosa (buccal and hard palate), using a separate cotton swab for each sampling site. After scraping, the cotton swab should be placed in the corresponding sterile tube, allowing the swab tip to soak in the corresponding saliva. 5 mL of phosphate buffer should be added, and the mixture should be stirred 50 times in the same direction to ensure that the residue on the cotton swab is fully dissolved in the buffer. The cotton swab should then be removed, and the sterile tube sealed.

[0044] Sample analysis: The concentrations of residual honeysuckle flower extract (chlorogenic acid), licorice extract (glycyrrhizic acid), and tea extract (catechins) in the oral cavity after rinsing were determined by high performance liquid chromatography (HPLC), and the mean and standard deviation of each test group were calculated. The data from the above tests are shown in Table 5.

[0045] Table 5 As shown in Table 5, in Examples 1-4, the residual amounts of honeysuckle extract, licorice extract, and tea extract in the oral cavity after rinsing were significantly higher than those in Comparative Examples 1 and 2. In Comparative Example 1, the natural plant extracts were not modified, and their residual amounts in the oral cavity were significantly lower than in the other examples. This indicates that the modification treatment with casein phosphopeptides and tannins can effectively prolong the retention time of natural plant extracts in the oral cavity. In Comparative Example 2, the pH of the system was adjusted to 7.0 during the modification treatment. Compared with the examples with a suitable pH range (4.5-6.0), its component residues were lower, indicating that suitable pH conditions have an important influence on the modification reaction and component retention effect. In summary, this invention modifies natural plant extracts by using casein phosphopeptides and tannic acid, thereby increasing the retention time of the natural plant extracts in the oral cavity. This ensures that the antibacterial components can exert their effects more persistently and fully, effectively inhibiting the growth of bacteria in the oral cavity. While ensuring gentleness and non-irritation, it achieves good antibacterial effects and has high application value and market prospects.

[0046] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A natural plant extract antibacterial mouthwash, characterized in that, Including the following ingredients: natural plant extracts, auxiliary functional agents, and water; The natural plant extracts include honeysuckle flower extract, licorice extract, and tea extract.

2. The antibacterial mouthwash made from natural plant extracts according to claim 1, characterized in that, The natural plant extract is modified with casein phosphopeptide and tannic acid.

3. The antibacterial mouthwash made from natural plant extracts according to claim 2, characterized in that, The modification steps of the natural plant extract are as follows: Honeysuckle flower extract, licorice extract, tea extract and water were mixed, casein phosphopeptide was added, and the mixture was stirred evenly. Then tannic acid was added to form a complex.

4. The antibacterial mouthwash made from natural plant extracts according to claim 3, characterized in that, The mass ratio of casein phosphopeptide, tannic acid and natural plant extract is 1-5:0.5-2.0:

100.

5. The antibacterial mouthwash made from natural plant extracts according to claim 3, characterized in that, In the modification process of the natural plant extract, after adding tannic acid solution, the pH is controlled to be 4.5-6.

0.

6. The antibacterial mouthwash made from natural plant extracts according to claim 3, characterized in that, In the modification process of the natural plant extract, after adding tannic acid solution, the temperature is controlled at 40-60℃.

7. The antibacterial mouthwash made from natural plant extracts according to claim 2, characterized in that, The modified natural plant extracts constitute 0.03-0.3% of the mouthwash ingredients by mass.

8. The antibacterial mouthwash made from natural plant extracts according to claim 1, characterized in that, The auxiliary functional additives include one or more of propylene glycol, malic acid, xylitol, menthol, and benzoic acid.

9. The antibacterial mouthwash made from natural plant extracts according to claim 8, characterized in that, The propylene glycol accounts for 0.01-15% of the mouthwash ingredients by mass, the malic acid accounts for 0.001-0.1% of the mouthwash ingredients by mass, the xylitol accounts for 0.01-3% of the mouthwash ingredients by mass, the menthol accounts for 0.01-0.5% of the mouthwash ingredients by mass, and the benzoic acid accounts for 0.01-0.5% of the mouthwash ingredients by mass.

10. A method for preparing a natural plant extract antibacterial mouthwash according to any one of claims 2-9, characterized in that, Includes the following steps: Add auxiliary functional agents and modified natural plant extracts to water step by step, stir and mix to obtain the finished mouthwash.

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