Modular adaptive stable positioning mechanism and separation sheet for plant tissue culture

By using a modular and adaptable stabilizing positioning mechanism, the problems of flexibility of the separator and uniformity of nutrient solution in plant tissue culture are solved, achieving stable fixation of the device and uniform distribution of nutrient solution, thereby improving plant growth consistency and experimental accuracy.

CN121176366APending Publication Date: 2025-12-23HEPUSI (JIANGSU) SCIENCE INSTRUMENT EQUIPMENT CO LTD
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Patent Information

Application Number
CN202511616783.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-06
Publication Date
2025-12-23

AI Technical Summary

Technical Problem

Existing plant tissue culture separation devices cannot flexibly adjust the separation method, resulting in uneven distribution of nutrient solution, affecting the consistency of plant growth and the accuracy of the experiment. In addition, the device is prone to shaking and damaging the root system.

Method used

It adopts a modular and adaptable stable positioning mechanism, which is firmly connected by the mounting bracket and the snap-fit ​​strip. The self-adaptive fixing column adapts to the size of the culture vessel, the separation plate and water-passing strip achieve uniform liquid distribution, and the mixing fan blades are combined to stir the solution.

Benefits of technology

It achieves stable fixation of the device, adapts to different culture vessel sizes, ensures uniform distribution of nutrient solution, avoids root damage, and improves plant growth consistency and experimental accuracy.

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Abstract

The invention relates to a modular adaptive stable positioning mechanism and a separation piece for plant tissue culture. According to the modular adaptive stable positioning mechanism and the separation piece for plant tissue culture, an incubator is separated through stable connection and installation of a mounting frame and a clamping strip, the size of the incubator is adapted through a self-adaptive fixing column, and the device is fixed to avoid damage to plants due to movement; the stable positioning mechanism is used for realizing regional liquid circulation through a separation plate water passing strip to guarantee uniform nutrients; and the separation sheet is used for plant tissue culture. The separation piece for plant tissue culture is characterized by comprising a mounting frame, a clamping strip, a separation plate, a water passing strip and a self-adaptive fixing column, the mounting frame is clamped on a connecting cylinder of the separation piece for plant tissue culture, the clamping strip is fixedly connected with the inner side face of the top end of the mounting frame, the clamping strip is clamped in an inserting strip of the connecting cylinder, and the water passing strip is fixedly connected with the inner side face of the top end of the mounting frame. And one side face of the separating plate is fixedly connected with the outer side face of the mounting frame, the separating plate is arranged on the outer side of the connecting cylinder, a plurality of water passing strips are arranged on the separating plate, and a self-adaptive fixing column is fixedly arranged on the other side face of the separating plate.
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Description

TECHNICAL FIELD

[0001] The modular adaptive stable fixing mechanism and plant tissue culture separation sheet relate to a stable fixing mechanism for fixing plants to reduce shaking, which is installed on a plant tissue culture separation sheet, and belong to the technical field of tissue culture. In particular, the stable fixing mechanism and plant tissue culture separation sheet separate the culture device through stable connection and installation of the mounting frame and the clamping strip, adapt to the size of the culture device through the self-adaptive fixing column, and fix the device to avoid movement and damage to the plants. The separation plate water passage strip realizes regional liquid flow to ensure uniform nutrients, and the stable fixing mechanism and plant tissue culture separation sheet. BACKGROUND

[0002] In the process of plant tissue culture, the roots of different plants are prone to entangle in the limited culture space. During subsequent separation and transplanting operations, the roots are easily damaged, which reduces the survival rate of plants and seriously affects the efficiency and quality of plant tissue culture. At present, the existing technology mainly uses fixed separation plates or separation nets to separate the roots of plants. These separation devices are usually designed in shape and size according to the size of the culture device, and are installed in the culture device in a fixed manner to divide the culture device space into several independent areas, thereby achieving the separation of plant roots. However, this existing plant root separation technology has many defects. On the one hand, due to its fixed structure, the separation method is single, and it is difficult to flexibly adjust the number and method of separation according to the culture needs of different types and different growth stages of plants, and the universality is poor. On the other hand, the existing separation device only has a simple space separation function and does not have a culture liquid mixing function. When new nutrient liquid is added to the culture device, it is difficult to ensure uniform distribution of the culture liquid in each separation area, which leads to differences in the nutrient conditions obtained by plants in different areas of the same culture device, thereby affecting the growth consistency of plants and the accuracy of test results, and it is difficult to meet the requirements of high-precision plant tissue culture tests.

[0003] Publication No. CN221930951U discloses a plant tissue culture separation sheet, which includes at least two strip-shaped separation sheet bodies. The separation sheet bodies are vertically inserted into the solid culture medium with the side down in use, and can be detachably connected in cross in the state in use. The end of the separation sheet body can be in contact with the inner wall of the culture bottle. The utility model can solve the problem that the roots of plants are prone to entangle after rooting. However, the above-mentioned plant tissue culture separation sheet can only divide the culture device into fixed separation areas, and cannot flexibly adjust the spatial layout according to the culture needs of different plants, which limits the optimization and innovation of the culture scheme to some extent. In addition, when nutrient liquid is injected into the culture device, the nutrient liquid is difficult to uniformly cover each separation area due to the lack of scientific flow guide design, which not only leads to differences in the nutrients obtained by plants at different positions, thereby affecting the accuracy of the test.

[0004] In order to improve the above problems, the applicant has filed another Chinese patent application for a plant tissue culture separation sheet, which can fix and limit the separation assembly through the support assembly, separate the plant roots through the separation assembly, and ensure the uniform plant growth environment by rotating and stirring the solution to uniformly disperse the liquid to each separated area. However, the above-mentioned plant tissue culture separation sheet only relies on the contact between the sliding ball and the bottom surface of the culture device, lacks reliable active fixing structure, and when the culture device is subjected to external force impact, liquid flow impact or accidental touch by the operator, the separation sheet is prone to displacement and shaking, cannot accurately position the separated area, changes the plant root growth space, and even causes root damage due to displacement and extrusion of the separation sheet. SUMMARY

[0005] In order to improve the above situation, the present application provides a stable fixing position mechanism and a plant tissue culture separation sheet, which separates the culture device by stably connecting and installing the installation frame and the clamping strip, fixes the device by adapting the size of the culture device and the self-adaptive fixing column to avoid damage to the plant, and realizes the flow of regional liquid to ensure uniform nutrients by the water passing strip of the separation plate.

[0006] The present application is realized as follows: the stable fixing position mechanism of the present application is composed of an installation frame, a clamping strip, a separation plate, a water passing strip and a self-adaptive fixing column, The installation frame is in U-shaped structure, and the installation frame is clamped on the connecting cylinder of the plant tissue culture separation sheet, Preferably, the two vertical plates of the installation frame are in arc-shaped structure, and the two inner side surfaces are respectively fitted with the outer side surface and the inner side surface of the connecting cylinder, The clamping strip is fixedly connected with the inner side surface of the top end of the installation frame, and the clamping strip is clamped in the insertion strip of the connecting cylinder, One side surface of the separation plate is fixedly connected with the outer side surface of the installation frame, and the separation plate is arranged outside the connecting cylinder, A plurality of water passing strips are formed on the separation plate, and the plurality of water passing strips are equidistantly arranged along the height direction of the separation plate, The other side surface of the separation plate is provided with a self-adaptive fixing column, Preferably, the self-adaptive fixing column is made of rubber material, and the self-adaptive fixing column is in hollow structure, Further, an auxiliary fixing rib is spirally wound in the self-adaptive fixing column, Further, the self-adaptive fixing column is replaced by a gradually changing self-adaptive fixing column, and the gradually changing self-adaptive fixing column gradually reduces in diameter near the bottom end.

[0007] The application discloses a separation sheet for plant tissue culture and a use method thereof. The separation sheet is characterized in that the separation components are clamped on the support component, the support component supports the multiple separation components and can mix the culture solution in the culture device, and the separation components separate the space in the culture device to avoid the plant roots from winding, The support component is composed of a support base, a placing groove, a sliding ball, a connecting cylinder, a second water through hole, an insertion strip and a stirring fan blade. The support base is in a disc structure. The placing groove is fixedly connected to the bottom surface of the support base and is arranged close to the side surface of the support base, the placing groove is in a spherical segment structure and is open at the bottom end, and a plurality of placing grooves are arranged at equal intervals in the circumferential direction of the support base. The sliding ball is connected to the placing groove in a ball-and-socket manner, and the placing groove is in one-to-one correspondence with the sliding ball. Preferably, the surface of the sliding ball is uniformly distributed with micron-level protrusions, the height of the protrusions is 10-50 microns, and the material of the protrusions is a biocompatible polymer material. The connecting cylinder is fixedly connected to the top surface of the support base, and the connecting cylinder is coaxially arranged on the support base. A plurality of second water through holes are uniformly distributed on the side surface of the connecting cylinder. A plurality of insertion strips are arranged close to the top end of the connecting cylinder, and the insertion strips are arranged at equal intervals in the circumferential direction of the connecting cylinder. The stirring fan blade is fixedly arranged on the inner side surface of the connecting cylinder, one side of the stirring fan blade is fixedly connected to the inner side surface of the connecting cylinder, extends to the other side along the central axis of the connecting cylinder, a plurality of stirring fan blades are arranged at equal intervals in the circumferential direction of the connecting cylinder, and the stirring fan blade does not intersect with the insertion strip. The separation component is composed of a separation blade and a first water through hole. The separation blade is in a square plate structure, a notch extending upward from the lower end is arranged close to one side surface of the separation blade, and the separation blade is plug-connected to the connecting cylinder through the notch and the insertion strip on the connecting cylinder. A plurality of first water through holes are uniformly distributed on the separation blade. Preferably, the first water through hole is a variable-diameter hole, the inlet diameter of the variable-diameter hole is 2-3 mm, the outlet diameter of the variable-diameter hole is 1-1.5 mm, and a hydrophilic coating is arranged on the hole wall. Further, a separation net is fixedly arranged on each side surface of the separation blade, so that the separation blade is convenient to clean. Further, a horizontal drag-reducing strip is arranged on the side of the stirring fan blade which does not contact the connecting cylinder, each stirring fan blade corresponds to a plurality of drag-reducing strips, and the drag-reducing strips are arranged at equal intervals in the height direction of the stirring fan blade.

[0008] The application also relates to a method for using a separation sheet for plant tissue culture, which comprises the following steps: (1) placing the support assembly in a culture device, with the disc-shaped support base stably contacting the bottom of the culture device to provide stable support for the whole device, and with the sliding ball in the ball socket being in contact with the bottom surface of the culture device, so that the friction between the sliding ball and the bottom surface of the culture device is reduced due to the micron-level protrusions uniformly distributed on the surface of the sliding ball, and the support assembly can be flexibly moved and adjusted in the culture device; (2) according to the size and quantity of the cultured plants, the separation leaf is inserted into the culture device through the side gap and the insertion strip at the top end of the connecting cylinder, so that the separation leaf is clamped on the connecting cylinder, and the space in the culture device is divided into multiple independent areas, and the roots of the plants are placed between two adjacent separation leaves, and the separation leaf plays a blocking role to avoid the roots of the plants from being intertwined; (3) when other nutrient liquid needs to be injected into the culture liquid, the nutrient liquid is poured into the connecting cylinder, the separation leaf is manually rotated, and the support assembly is rotated as a whole in the culture device, at this time, the stirring fan blade fixed on the inner side of the connecting cylinder is rotated, the nutrient liquid in the connecting cylinder and the original culture liquid are stirred, and the two are fully mixed, and due to the first water hole and the second water hole, the mixed liquid passes through the second water hole and the first water hole in turn and is uniformly dispersed into each area in the culture device, so that the liquid composition and concentration soaked by all the plants in the culture device are the same, a consistent nutrient environment is provided for the growth of the plants, and the accuracy of the plant tissue culture test is ensured. Advantages

[0009] I. The self-adaptive fixing column closely contacts the inner wall of the culture device, effectively prevents the device from shaking and moving during the culture process, and provides a stable growth environment for the plant tissue.

[0010] II. The device can adapt to small changes in the size of the culture device, and the number of installation racks can be increased or decreased to adjust the partition according to the plant culture requirements, thereby improving the versatility and use flexibility of the device. DETAILED DESCRIPTION

[0011] Figure 1 FIG. 1 is a perspective view of a separation sheet for plant tissue culture according to the present application; Figure 2 FIG. 2 is a perspective view of a separation sheet for plant tissue culture according to the present application; Figure 3 FIG. 3 is a perspective view of a separation sheet for plant tissue culture according to the present application, which is an embodiment 2; Figure 4 FIG. 4 is a perspective view of a separation sheet for plant tissue culture according to the present application, which is an embodiment 3; Figure 5It is a modular adaptive stable fixing position mechanism and a three-dimensional structure diagram of a separation sheet for plant tissue culture; Figure 6 It is a three-dimensional structure diagram of a modular adaptive stable fixing position mechanism and a separation sheet for plant tissue culture, which only shows the structure of the mounting frame; Figure 7 It is a three-dimensional structure diagram of a modular adaptive stable fixing position mechanism and a separation sheet for plant tissue culture, which only shows the structure of the adaptive fixing column; Figure 8 It is a three-dimensional structure diagram of a modular adaptive stable fixing position mechanism and a separation sheet for plant tissue culture. DRAWINGS

[0012] Among them are: support base (1), placing groove (2), sliding ball (3), separation blade (4), first water hole (5), connecting cylinder (6), insertion strip (7), second water hole (8), stirring fan blade (9), separation net (10), drag reduction strip (11), adaptive fixing column (12), separation plate (13), water passing strip (14), mounting frame (15), clamping strip (16), auxiliary fixing rib (17), gradually changing adaptive fixing column (18). DETAILED DESCRIPTION Example 1

[0013] The modular adaptive stable fixing position mechanism of the application is composed of a mounting frame (15), a clamping strip (16), a separation plate (13), a water passing strip (14) and an adaptive fixing column (12), The mounting frame (15) is in a U-shaped structure, and the mounting frame (15) is clamped on the connecting cylinder (6), Preferably, the two vertical plates of the mounting frame (15) are in an arc-shaped structure, and the two inner sides are respectively fitted with the outer side and the inner side of the connecting cylinder (6), The clamping strip (16) is fixedly connected with the inner side of the top end of the mounting frame (15), and the clamping strip (16) is clamped in the insertion strip (7) of the connecting cylinder (6), The separation plate (13) is fixedly connected with the outer side of the mounting frame (15), and the separation plate (13) is arranged outside the connecting cylinder (6), A plurality of water passing strips (14) are formed on the separation plate (13), and the plurality of water passing strips (14) are equidistantly arranged along the height direction of the separation plate (13), The other side of the separation plate (13) is provided with an adaptive fixing column (12), Preferably, the adaptive fixing column (12) is made of rubber, and the adaptive fixing column (12) is in a hollow structure, In use, the connecting cylinder (6) is placed in the culture device, the mounting frame (15) can be tightly clamped on the connecting cylinder (6), and the inner sides of the two vertical plates are respectively attached to the outer side and the inner side of the connecting cylinder (6) to form a stable initial mounting form. During the clamping of the mounting frame (15) to the connecting cylinder (6), the clamping strip (16) will be accurately clamped into the insertion strip (7) of the connecting cylinder (6). When placing a proper number of mounting frames (15) according to the size and number of plants to be cultured, the multiple separation plates (13) will divide the culture device into multiple independent areas, facilitating the partitioned culture of different types or different growth stages of plant tissues. After the device is installed, the self-adaptive fixing column (12) will be attached to the inner side of the culture device. Due to the good elasticity of the rubber material and the hollow structure which provides certain deformation ability, the self-adaptive fixing column (12) can adapt to the small changes in the size of the culture device. Through the extrusion force generated by the elastic deformation of the self-adaptive fixing column (12), the entire device is stably fixed in the culture device, avoiding the shaking of the device during the culture process and preventing the accidental injury of plant tissues caused by the shaking of the device. Example 2

[0014] The difference between this embodiment and Example 1 is that the self-adaptive fixing column (12) is internally spirally wound with an auxiliary fixing rib (17). In use, the auxiliary fixing rib (17) provides continuous and stable support for the self-adaptive fixing column (12). Even in the case of large deformation of the self-adaptive fixing column (12), the auxiliary fixing rib (17) can effectively prevent it from over-deforming or rebounding, ensuring that the self-adaptive fixing column (12) is always tightly attached to the side of the culture device, stably fixing the entire device and avoiding device shaking caused by insufficient support of the self-adaptive fixing column (12), thereby providing reliable structural protection for plant tissue culture. Example 3

[0015] The difference between this embodiment and Example 1 is that the self-adaptive fixing column (12) is replaced by a gradually changing self-adaptive fixing column (18). The gradually changing self-adaptive fixing column (18) gradually decreases in diameter near the bottom end. In use, its pointed end shape is easier to insert into the interior of the culture device, making it easy to place the fixing column in the designated position of the culture device during installation, greatly reducing the installation difficulty and saving labor and time costs. The mounting frame (15) is designed in a U shape with the two vertical plates being arc-shaped structures, which can be tightly clamped on the connecting cylinder (6), and the inner sides of the two vertical plates are respectively attached to the outer side and the inner side of the connecting cylinder (6) to form a stable initial mounting form, greatly enhancing the installation stability of the entire auxiliary fixing structure on the connecting cylinder (6) and effectively reducing the risk of the mounting frame (15) falling off or shifting from the connecting cylinder (6) due to external impact or improper operation during the culture process. The design of the plurality of water passing strips (14) arranged equidistantly along the height direction on the separation plate (13) can ensure the liquid circulation between the regions in the culture device during the culture process, and the nutrient-rich culture solution and the oxygen and other gases dissolved therein can be uniformly distributed to each culture region through the water passing strips (14), so that the liquid circulation is not poor due to the region separation, and each culture region can be in a balanced nutrition and good gas exchange environment, promoting the healthy growth of the plant tissue and reducing the problems of poor growth, slow development and even death caused by unbalanced nutrition or oxygen deficiency; The self-adaptive fixing column (12) is made of rubber material and has a hollow structure, so that the device can be stably fixed in the culture device through the extrusion force generated by the elastic deformation, and the shaking of the device during the culture process is effectively avoided. The device can be stably connected and installed on the culture device through the mounting frame (15) and the clamping strip (16), the size of the culture device can be adapted through the self-adaptive fixing column (12), the device is fixed to avoid movement and damage to the plant, and the liquid circulation in the region is realized through the water passing strip (14) of the separation plate (13) to ensure uniform nutrition.

[0016] It should be noted that the above-mentioned auxiliary fixing structure needs to be installed on the following plant tissue culture separation sheet. The plant tissue culture separation sheet and the using method thereof are realized as follows: The separation assembly is clamped on the support assembly, the support assembly supports the plurality of separation assemblies, and can mix the culture solution in the culture device, and the separation assembly separates the space in the culture device to avoid the entanglement of the plant roots. The support assembly is composed of a support base (1), a placing groove (2), a sliding ball (3), a connecting barrel (6), a second water passing hole (8), an insertion strip (7) and a stirring fan blade (9). The support base (1) has a disc-shaped structure. The placing groove (2) is fixedly connected with the bottom surface of the support base (1) and is arranged close to the side surface of the support base (1), the placing groove (2) has a spherical segment-shaped structure and an open structure at the bottom end, and a plurality of placing grooves (2) are arranged equidistantly along the circumference of the support base (1). The sliding ball (3) is connected with the placing groove (2) in a ball-and-socket manner, and the placing groove (2) corresponds to the sliding ball (3) one by one. Preferably, the surface of the sliding ball (3) is uniformly distributed with micron-sized protrusions, the height of which is 10-50 μm, and the material is a biocompatible polymer. The connecting cylinder (6) is fixedly connected to the top surface of the support base (1), and the connecting cylinder (6) and the support base (1) are coaxially arranged. The connecting cylinder (6) has multiple second water passage holes (8) evenly distributed on its side. The connecting cylinder (6) has multiple insert strips (7) near its top end, and the insert strips (7) are arranged at equal intervals along the circumference of the connecting cylinder (6). The stirring blades (9) are fixedly placed on the inner side of the connecting cylinder (6). One side of the stirring blades (9) is fixedly connected to the inner side of the connecting cylinder (6) and extends to the other side along the central axis of the connecting cylinder (6). Multiple stirring blades (9) are arranged equidistantly along the circumference of the connecting cylinder (6). The stirring blades (9) do not intersect with the insert strips (7). The separation assembly consists of a separating blade (4) and a first water passage (5). The separator blade (4) has a square plate-like structure. Near one side of the separator blade (4), there is a notch extending upwards from the bottom. The separator blade (4) is connected to the connecting cylinder (6) by inserting and pulling through the notch with the insert strip (7) on the connecting cylinder (6). The separator blade (4) has multiple first water passage holes (5) evenly distributed on it. Preferably, the first water passage (5) is a variable diameter hole with an inlet diameter of 2-3 mm and an outlet diameter of 1-1.5 mm, and the hole wall is provided with a hydrophilic coating. This invention also relates to a method of using a separation slide for plant tissue culture, comprising the following steps: (1) Place the support assembly in the incubator. The disc-shaped support base (1) makes stable contact with the bottom of the incubator, providing stable support for the entire device. In the placement groove (2) on the bottom surface of the support base (1), the sliding ball (3) connected by the ball socket contacts the bottom surface of the incubator. Due to the micron-level protrusions evenly distributed on its surface, the friction with the bottom surface of the incubator is reduced, allowing the support assembly to move flexibly and adjust its position within the incubator. (2) According to the size and number of the plants to be cultivated, the dividing leaf (4) is inserted through the side notch along the insertion strip (7) at the top of the connecting tube (6) so that the dividing leaf (4) is stuck on the connecting tube (6), thereby dividing the space inside the culture vessel into multiple independent areas. The plant roots are placed between two adjacent dividing leaves (4). The dividing leaf (4) acts as a barrier to prevent the plant roots from tangling together. (3) When other nutrient liquid needs to be injected into the culture solution, pour the nutrient liquid into the connecting cylinder (6), manually rotate the separation blade (4), and drive the support assembly to rotate as a whole in the culture device. At this time, the stirring fan blade (9) fixed on the inner side of the connecting cylinder (6) rotates with it, and the nutrient liquid in the connecting cylinder (6) and the original culture solution are stirred and mixed, so that the two are fully mixed. Due to the arrangement of the first water hole (5) and the second water hole (8), the mixed liquid passes through the second water hole (8) and the first water hole (5) in turn, and is uniformly dispersed into each separated area of the culture device, ensuring that the liquid composition and concentration soaked by all plants in the culture device are the same, providing a consistent nutrient environment for plant growth, and ensuring the accuracy of plant tissue culture test; Example 2

[0017] The difference between this embodiment and example 1 is that a separation net (10) is fixed on each side of the separation blade (4). During use, the separation net (10) can effectively block the plant roots from passing through the first water hole (5), avoid the entanglement of plant roots in different areas, provide independent growth space for plant roots, and ensure the healthy growth of plants. At the same time, it can intercept impurities and avoid their adsorption on the separation blade (4), significantly reducing the cleaning difficulty of the separation blade (4); Example 3

[0018] The difference between this embodiment and example 1 is that the stirring fan blade (9) is provided with a horizontal resistance-reducing strip (11) on the side not in contact with the connecting cylinder (6). Each stirring fan blade (9) corresponds to multiple resistance-reducing strips (11), and multiple resistance-reducing strips (11) are equidistantly arranged along the height direction of the stirring fan blade (9). During use, when the separation piece rotates as a whole, the resistance-reducing strip (11) cuts the liquid when the fan blade rotates, forming a micro-scale turbulent flow, breaking the laminar flow state, and making the nutrient substances and the culture solution more fully mixed; The surface of the sliding ball (3) is uniformly distributed with micron-level protrusions with a height of 10-50 μm. The design of the biocompatible polymer material can reduce the contact area between the sliding ball (3) and the bottom surface of the culture device, reduce friction, and prevent microorganisms from attaching and breeding on the surface of the sliding ball, avoiding pollution to the culture solution; The first water hole (5) is a variable-diameter hole with an inlet diameter of 2-3 mm and an outlet diameter of 1-1.5 mm, and the hole wall is provided with a hydrophilic coating. The variable-diameter hole structure increases the flow rate when the liquid passes through, forming a jet effect, promoting the exchange of culture solution between the separated areas, and the hydrophilic coating can prevent organic matter in the culture solution from adhering to the hole wall, avoiding the blockage of the water hole; The stirring fan blades (9) are fixed on the inner side of the connecting cylinder (6), one side of the stirring fan blades (9) is fixedly connected with the inner side of the connecting cylinder (6), extends to the other side along the central axis of the connecting cylinder (6), and a plurality of the stirring fan blades (9) are arranged at equal intervals along the circumference of the connecting cylinder (6). When the stirring fan blades (9) are rotated by the stirring blade (4), the fan blades at different positions can apply force to the liquid from various directions, so that the nutrient liquid and the original culture solution in the connecting cylinder (6) are fully stirred, the stirring dead angle is avoided, the uniformity of the liquid mixing is greatly improved, and a more balanced nutrient environment is provided for the plants. The separation assembly can separate the roots of the plants, and the combination of the supporting assembly and the separation assembly can rotate and stir the solution to uniformly disperse the liquid to each separation area, so that the growth environment of the plants is consistent.

[0019] It should be noted that, unless otherwise specified and limited, the terms "placed", "connected", and "connected" should be understood broadly, for example, it can be a fixed connection mode such as folding connection, rivet connection, pin connection, bonding connection and welding connection, or a detachable connection mode such as threaded connection, buckle connection and hinge connection, or integral connection, or electrical connection, or direct connection, or indirect connection through an intermediate medium, or communication between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0020] It should be further pointed out that, in the description of the above specific embodiments, only the differences between them and other embodiments are described for simplicity and clarity, but those skilled in the art should know that the above specific embodiments themselves are also independent technical solutions.

Claims

1. A modular, adaptable, and stable positioning mechanism, comprising a mounting frame, a snap-fit ​​strip, a separation plate, a water-passing strip, and an adaptive fixing column, wherein the mounting frame is snapped onto the connecting cylinder of the plant tissue culture separation plate, the snap-fit ​​strip is fixedly connected to the inner side of the top of the mounting frame, the snap-fit ​​strip is snapped into the insertion strip of the connecting cylinder, one side of the separation plate is fixedly connected to the outer side of the mounting frame, the separation plate is located on the outside of the connecting cylinder, the separation plate has multiple water-passing strips, and an adaptive fixing column is fixedly placed on the other side of the separation plate.

2. The modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The adaptive fixing column is spirally wound with auxiliary fixing ribs.

3. The modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The adaptive fixing column is replaced with a gradient adaptive fixing column, the diameter of which gradually decreases near the bottom.

4. The modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The mounting bracket has a U-shaped structure.

5. A modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The mounting bracket has two arc-shaped vertical plates.

6. A modular adaptable stabilizing positioning mechanism according to claim 5, characterized in that... The two inner sides of the mounting bracket are respectively attached to the outer and inner sides of the connecting cylinder.

7. A modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The multiple water-passing strips are arranged at equal intervals along the height direction of the separation plate.

8. The modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The adaptive fixing column is made of rubber.

9. A modular adaptable stabilizing positioning mechanism according to claim 8, characterized in that... The adaptive fixed column is a hollow structure.

10. A modular adaptable stabilizing positioning mechanism according to claim 1, characterized in that... The plant tissue culture separation slide includes a support assembly and multiple separation components. The separation components are mounted on the support assembly, which supports the multiple separation components and simultaneously mixes the culture medium in the culture vessel. The separation components divide the space in the culture vessel to prevent plant roots from tangling. The support assembly consists of a support base, placement slots, sliding balls, a connecting cylinder, a second water passage, insertion strips, and stirring blades. The support base has a disc-shaped structure. The placement slots are fixedly connected to the bottom surface of the support base and are located near the side of the support base. The placement slots have a spherical notch structure with an open bottom. Multiple placement slots are arranged equidistantly along the circumference of the support base. The sliding balls are connected to the notches in the placement slots, with each placement slot corresponding to a sliding ball. The surface of each sliding ball has uniformly distributed micron-sized protrusions, 10-50 μm in height, made of a biocompatible polymer material. The connecting cylinder is fixedly connected to the top surface of the support base. The connecting cylinder and the supporting base are coaxially arranged. Multiple second water passages are evenly distributed on the side of the connecting cylinder. Multiple insertion strips are opened near the top of the connecting cylinder. The insertion strips are equidistantly arranged along the circumference of the connecting cylinder. The stirring blades are fixedly placed on the inner side of the connecting cylinder. One side of the stirring blade is fixedly connected to the inner side of the connecting cylinder and extends towards the central axis of the connecting cylinder to the other side. Multiple stirring blades are equidistantly arranged along the circumference of the connecting cylinder. The stirring blades and the insertion strips do not intersect. The separation component consists of a separating blade and a first water passage. The separating blade has a square plate structure. A notch is opened near one side of the separating blade, extending upward from the bottom. The separating blade is plugged into and pulled into the connecting cylinder through the notch and the insertion strip on the connecting cylinder. Multiple first water passages are evenly distributed on the separating blade. The first water passage is a variable diameter hole with an inlet diameter of 2-3 mm and an outlet diameter of 1-1.5 mm. The hole wall is provided with a hydrophilic coating.

Citation Information

Patent Citations

  • Separating piece for plant tissue culture

    CN221930951U